Rroxscaffold_3G00229740

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0009185
Physical Location & Seq
Reverse (-)
14284311 .. 14287110
2800 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00229740.1

Sequence Viewer

Length: 1122 bp
ATGGCTAAGCACTACTTTAGGCTATTTTCTTCTGCTTTGCTTGTTTTGTCCTTCATGGTGCTGCAGGCTGAAGTAGTTTGGGGTCAAGGTGGTACTGAAAGGAGGAAAGAAATGGTGCCCGCAATGTTCATATTCGGAGACTCTCTGATTGACAATGGCAACAACAACAACCTCCCTTCATTTGCCAAGGCCAACTATCCCCCCTATGGAATCGATTTCAATGGCGGACCAACTGGTCGTTTTAGCAATGGTTACACCATGGTTGATGAAATAGCTGAATTGCTAGGACTTCCTTTAATTCCTGCGTACTCTGAAGCTTCTGGAGATCAAGTGCTTCATGGTGTCAATTATGCATCTGCAGCCGCTGGAATCCTTGATATCACTGGCAGAAACTTTGTGGGTCGCATACCCTTTGGTCAACAAATAAGCAACTTCCAGACTACAGTTGATCAGATAACGGAGACTCTGGGTGCAGATGATGTTGCCCGGGCCATTGCAAAGTGCATATTCTTTGTTGGAATGGGCAGCAATGACTACCTAAACAATTACCTTATGCCCAACTATAACACCAAGAATCAATACAATGCTCAACAATTTGCTGATCTCTTGGCTCAACAATACACTCAGCAACTCACTAGGCTCTACAATCTTGGTGCTCGAAGATTTGTTATTGCTGGAGTTGGGAGGATGGGTTGCATACCAAGTATCTTAGCCCAAAGTCCAAATGGAAGCTGCTCAGAGGACGTCAATCGCCTTGTTCTACCCTTCAACGCAAATGTGAGGACAATGATCAACAATCTTAATACCAACTTACCTGGTTCGAGGTTCATTTACATTGACATTGCTCGAATGTTTGAAGATGTCGTCACCAATGCTAGAGCTTATGGATTCAGTGTTGCGAATCGTGGATGCTGTGGTATTGGGCGTAACAGAGGGCAAATTACATGTCTTCCAATGCAAACACCGTGCCCAAATCGTGACCAGTACGTGTTCTGGGATGCATATCACCCAACTGCAGCTGTGAACATCTTAATTGGCAGGAAAGCTTTCAGTGGAGACCCTAGCCAGGTTTTCCCCATGAACATACAGCAGCTTGCCACTCTTAACATTGAAACCAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

373

Amino Acids

41.08

Weight (kDa)

6.82

Isoelectric Point (pI)

32.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 43 - 346 3.4e-35 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 234
AatII GACGTC 1 cut(s) 747
AccB1I GGYRCC 1 cut(s) 115
AciI CCGC 3 cut(s) 120, 225, 363
AcuI CTGAAG 2 cut(s) 90, 333
AcyI GRCGYC 1 cut(s) 744
AfaI GTAC 3 cut(s) 94, 308, 986
AfiI CCNNNNNNNGG 2 cut(s) 206, 1066
AflIII ACRYGT 2 cut(s) 944, 987
AgsI TTSAA 4 cut(s) 220, 769, 857, 1112
AjnI CCWGG 2 cut(s) 814, 1065
AluBI AGCT 7 cut(s) 275, 317, 732, 881, 1019, 1046, 1093
AluI AGCT 7 cut(s) 275, 317, 732, 881, 1019, 1046, 1093
Alw21I GWGCWC 1 cut(s) 658
Alw26I GTCTC 3 cut(s) 132, 455, 1050
AlwNI CAGNNNCTG 1 cut(s) 365
Ama87I CYCGRG 1 cut(s) 486
AoxI GGCC 2 cut(s) 189, 489
ApeKI GCWGC 6 cut(s) 61, 359, 525, 732, 1016, 1090
ArsI GACNNNNNNTTYG 2 cut(s) 931, 963
Asp700I GAANNNNTTC 1 cut(s) 1046
AspS9I GGNCC 2 cut(s) 227, 489
AsuC2I CCSGG 2 cut(s) 487, 488
AsuHPI GGTGA 2 cut(s) 859, 998
AvaI CYCGRG 1 cut(s) 486
AvaII GGWCC 1 cut(s) 227
BaeGI GKGCMC 2 cut(s) 120, 971
BanI GGYRCC 1 cut(s) 115
BbsI GAAGAC 1 cut(s) 941
Bbv12I GWGCWC 1 cut(s) 658
BbvI GCAGC 6 cut(s) 48, 371, 537, 719, 1028, 1102
BccI CCATC 1 cut(s) 682
BciT130I CCWGG 2 cut(s) 816, 1067
BclI TGATCA 2 cut(s) 448, 789
BcnI CCSGG 2 cut(s) 487, 488
BcoDI GTCTC 3 cut(s) 132, 455, 1050
BfaI CTAG 4 cut(s) 284, 636, 876, 1062
BfmI CTRYAG 4 cut(s) 62, 357, 441, 1014
BisI GCNGC 7 cut(s) 62, 360, 363, 526, 733, 1017, 1091
BlpI GCTNAGC 1 cut(s) 6
BlsI GCNGC 7 cut(s) 63, 361, 364, 527, 734, 1018, 1092
Bme1390I CCNGG 4 cut(s) 487, 488, 816, 1067
Bme18I GGWCC 1 cut(s) 227
BmeT110I CYCGRG 1 cut(s) 486
BmgT120I GGNCC 2 cut(s) 227, 489
BmiI GGNNCC 1 cut(s) 117
BmrFI CCNGG 4 cut(s) 487, 488, 816, 1067
BmsI GCATC 3 cut(s) 362, 899, 988
BpiI GAAGAC 1 cut(s) 941
BpmI CTGGAG 2 cut(s) 342, 696
Bpu1102I GCTNAGC 1 cut(s) 6
BpuMI CCSGG 2 cut(s) 487, 488
Bsa29I ATCGAT 1 cut(s) 213
BsaAI YACGTR 1 cut(s) 988
BsaBI GATNNNNATC 1 cut(s) 1002
BsaHI GRCGYC 1 cut(s) 744
BsaI GGTCTC 1 cut(s) 1050
BsaJI CCNNGG 3 cut(s) 186, 258, 486
BsaXI ACNNNNNCTCC 4 cut(s) 315, 345, 676, 706
Bsc4I CCNNNNNNNGG 2 cut(s) 206, 1066
Bse1I ACTGG 3 cut(s) 238, 388, 982
Bse3DI GCAATG 5 cut(s) 129, 253, 492, 535, 840
Bse8I GATNNNNATC 1 cut(s) 1002
BseBI CCWGG 2 cut(s) 816, 1067
BseCI ATCGAT 1 cut(s) 213
BseDI CCNNGG 3 cut(s) 186, 258, 486
BseGI GGATG 3 cut(s) 693, 914, 1003
BseJI GATNNNNATC 1 cut(s) 1002
BseLI CCNNNNNNNGG 2 cut(s) 206, 1066
BseMI GCAATG 5 cut(s) 129, 253, 492, 535, 840
BseMII CTCAG 2 cut(s) 638, 750
BseNI ACTGG 3 cut(s) 238, 388, 982
BseSI GKGCMC 2 cut(s) 120, 971
BseXI GCAGC 6 cut(s) 48, 371, 537, 719, 1028, 1102
BsgI GTGCAG 1 cut(s) 492
BshFI GGCC 2 cut(s) 191, 491
BshNI GGYRCC 1 cut(s) 115
BshVI ATCGAT 1 cut(s) 213
BsiHKAI GWGCWC 1 cut(s) 658
BsiHKCI CYCGRG 1 cut(s) 486
BsiSI CCGG 1 cut(s) 487
BslI CCNNNNNNNGG 2 cut(s) 206, 1066
BsmAI GTCTC 3 cut(s) 132, 455, 1050
BsnI GGCC 2 cut(s) 191, 491
Bso31I GGTCTC 1 cut(s) 1050
BsoBI CYCGRG 1 cut(s) 486
Bsp1286I GDGCHC 3 cut(s) 120, 658, 971
Bsp143I GATC 4 cut(s) 325, 448, 601, 789
Bsp1720I GCTNAGC 1 cut(s) 6
Bsp19I CCATGG 1 cut(s) 258
BspACI CCGC 3 cut(s) 120, 225, 363
BspANI GGCC 2 cut(s) 191, 491
BspCNI CTCAG 2 cut(s) 637, 749
BspDI ATCGAT 1 cut(s) 213
BspLI GGNNCC 1 cut(s) 117
BspMAI CTGCAG 3 cut(s) 66, 361, 1018
BspT107I GGYRCC 1 cut(s) 115
BspTNI GGTCTC 1 cut(s) 1050
BsrDI GCAATG 5 cut(s) 129, 253, 492, 535, 840
BsrI ACTGG 3 cut(s) 238, 388, 982
BssECI CCNNGG 3 cut(s) 186, 258, 486
BssMI GATC 4 cut(s) 325, 448, 601, 789
BssNI GRCGYC 1 cut(s) 744
BssT1I CCWWGG 2 cut(s) 186, 258
Bst2UI CCWGG 2 cut(s) 816, 1067
Bst4CI ACNGT 2 cut(s) 445, 966
BstACI GRCGYC 1 cut(s) 744
BstBAI YACGTR 1 cut(s) 988
BstC8I GCNNGC 3 cut(s) 66, 120, 1095
BstDEI CTNAG 4 cut(s) 6, 624, 709, 736
BstDSI CCRYGG 1 cut(s) 258
BstF5I GGATG 3 cut(s) 693, 914, 1003
BstKTI GATC 4 cut(s) 328, 451, 604, 792
BstMAI GTCTC 3 cut(s) 132, 455, 1050
BstMBI GATC 4 cut(s) 325, 448, 601, 789
BstMWI GCNNNNNNNGC 1 cut(s) 359
BstNI CCWGG 2 cut(s) 816, 1067
BstNSI RCATGY 1 cut(s) 948
BstSCI CCNGG 4 cut(s) 485, 486, 814, 1065
BstSFI CTRYAG 4 cut(s) 62, 357, 441, 1014
BstSLI GKGCMC 2 cut(s) 120, 971
BstV1I GCAGC 6 cut(s) 48, 371, 537, 719, 1028, 1102
BstV2I GAAGAC 1 cut(s) 941
Bsu15I ATCGAT 1 cut(s) 213
BsuRI GGCC 2 cut(s) 191, 491
BsuTUI ATCGAT 1 cut(s) 213
BtgI CCRYGG 1 cut(s) 258
BtsCI GGATG 3 cut(s) 693, 914, 1003
BtsIMutI CAGTG 3 cut(s) 381, 898, 1057
Cac8I GCNNGC 3 cut(s) 66, 120, 1095
CaiI CAGNNNCTG 1 cut(s) 365
Cfr13I GGNCC 2 cut(s) 227, 489
Cfr9I CCCGGG 1 cut(s) 486
ClaI ATCGAT 1 cut(s) 213
CsiI ACCWGGT 1 cut(s) 814
Csp6I GTAC 3 cut(s) 93, 307, 985
CviAII CATG 5 cut(s) 55, 259, 338, 945, 1078
CviQI GTAC 3 cut(s) 93, 307, 985
DdeI CTNAG 4 cut(s) 6, 624, 709, 736
DpnI GATC 4 cut(s) 327, 450, 603, 791
DpnII GATC 4 cut(s) 325, 448, 601, 789
DrdI GACNNNNNNGTC 1 cut(s) 234
DseDI GACNNNNNNGTC 1 cut(s) 234
EciI GGCGGA 1 cut(s) 240
Eco130I CCWWGG 2 cut(s) 186, 258
Eco31I GGTCTC 1 cut(s) 1050
Eco32I GATATC 1 cut(s) 379
Eco47I GGWCC 1 cut(s) 227
Eco57I CTGAAG 2 cut(s) 90, 333
Eco88I CYCGRG 1 cut(s) 486
EcoRII CCWGG 2 cut(s) 814, 1065
EcoRV GATATC 1 cut(s) 379
EcoT14I CCWWGG 2 cut(s) 186, 258
EcoT22I ATGCAT 2 cut(s) 355, 1003
ErhI CCWWGG 2 cut(s) 186, 258
FaeI CATG 5 cut(s) 58, 262, 341, 948, 1081
FalI AAGNNNNNCTT 1 cut(s) 31
FatI CATG 5 cut(s) 54, 258, 337, 944, 1077
FauI CCCGC 1 cut(s) 127
FbaI TGATCA 2 cut(s) 448, 789
Fnu4HI GCNGC 7 cut(s) 62, 360, 363, 526, 733, 1017, 1091
FokI GGATG 3 cut(s) 700, 921, 1010
Fsp4HI GCNGC 7 cut(s) 62, 360, 363, 526, 733, 1017, 1091
FspBI CTAG 4 cut(s) 284, 636, 876, 1062
GluI GCNGC 7 cut(s) 62, 360, 363, 526, 733, 1017, 1091
GsuI CTGGAG 2 cut(s) 342, 696
HaeIII GGCC 2 cut(s) 191, 491
HapII CCGG 1 cut(s) 487
Hin1I GRCGYC 1 cut(s) 744
Hin1II CATG 5 cut(s) 58, 262, 341, 948, 1081
HincII GTYRAC 1 cut(s) 419
HindII GTYRAC 1 cut(s) 419
HindIII AAGCTT 2 cut(s) 315, 1044
HinfI GANTC 7 cut(s) 140, 210, 369, 463, 574, 888, 901
HpaII CCGG 1 cut(s) 487
HphI GGTGA 2 cut(s) 859, 998
Hpy166II GTNNAC 2 cut(s) 419, 1024
Hpy188I TCNGA 5 cut(s) 137, 147, 313, 453, 739
Hpy188III TCNNGA 3 cut(s) 321, 436, 977
Hpy8I GTNNAC 2 cut(s) 419, 1024
HpyAV CCTTC 3 cut(s) 61, 186, 775
HpyCH4III ACNGT 2 cut(s) 445, 966
HpyCH4IV ACGT 2 cut(s) 744, 987
HpyF10VI GCNNNNNNNGC 1 cut(s) 359
HpyF3I CTNAG 4 cut(s) 6, 624, 709, 736
HpySE526I ACGT 2 cut(s) 744, 987
Hsp92I GRCGYC 1 cut(s) 744
Hsp92II CATG 5 cut(s) 58, 262, 341, 948, 1081
Ksp22I TGATCA 2 cut(s) 448, 789
Kzo9I GATC 4 cut(s) 325, 448, 601, 789
Lsp1109I GCAGC 6 cut(s) 48, 371, 537, 719, 1028, 1102
LweI GCATC 3 cut(s) 362, 899, 988
MabI ACCWGGT 1 cut(s) 814
MaeI CTAG 4 cut(s) 284, 636, 876, 1062
MaeII ACGT 2 cut(s) 744, 987
MaeIII GTNAC 4 cut(s) 251, 865, 926, 977
MalI GATC 4 cut(s) 327, 450, 603, 791
MboI GATC 4 cut(s) 325, 448, 601, 789
MboII GAAGA 4 cut(s) 21, 672, 869, 941
MhlI GDGCHC 3 cut(s) 120, 658, 971
MluCI AATT 8 cut(s) 278, 297, 346, 544, 593, 939, 1032, 1117
MlyI GAGTC 2 cut(s) 134, 457
MmeI TCCRAC 1 cut(s) 496
MnlI CCTC 7 cut(s) 96, 182, 678, 733, 774, 816, 926
Mph1103I ATGCAT 2 cut(s) 355, 1003
MroXI GAANNNNTTC 1 cut(s) 1046
MseI TTAA 4 cut(s) 296, 801, 1031, 1104
MspA1I CMGCKG 2 cut(s) 365, 1019
MspI CCGG 1 cut(s) 487
MspR9I CCNGG 4 cut(s) 487, 488, 816, 1067
MvaI CCWGG 2 cut(s) 816, 1067
MwoI GCNNNNNNNGC 1 cut(s) 359
NciI CCSGG 2 cut(s) 487, 488
NcoI CCATGG 1 cut(s) 258
NdeII GATC 4 cut(s) 325, 448, 601, 789
NlaIII CATG 5 cut(s) 58, 262, 341, 948, 1081
NlaIV GGNNCC 1 cut(s) 117
NmuCI GTSAC 2 cut(s) 865, 977
NsiI ATGCAT 2 cut(s) 355, 1003
NspI RCATGY 1 cut(s) 948
PciI ACATGT 1 cut(s) 944
PdmI GAANNNNTTC 1 cut(s) 1046
PfeI GAWTC 5 cut(s) 210, 369, 574, 888, 901
PkrI GCNGC 7 cut(s) 63, 361, 364, 527, 734, 1018, 1092
PleI GAGTC 2 cut(s) 134, 457
PpsI GAGTC 2 cut(s) 134, 457
Ppu21I YACGTR 1 cut(s) 988
PscI ACATGT 1 cut(s) 944
Psp6I CCWGG 2 cut(s) 814, 1065
PspGI CCWGG 2 cut(s) 814, 1065
PspN4I GGNNCC 1 cut(s) 117
PspPI GGNCC 2 cut(s) 227, 489
PstI CTGCAG 3 cut(s) 66, 361, 1018
PstNI CAGNNNCTG 1 cut(s) 365
PvuII CAGCTG 1 cut(s) 1019
RsaI GTAC 3 cut(s) 94, 308, 986
RsaNI GTAC 3 cut(s) 93, 307, 985
SaqAI TTAA 4 cut(s) 296, 801, 1031, 1104
SatI GCNGC 7 cut(s) 62, 360, 363, 526, 733, 1017, 1091
Sau3AI GATC 4 cut(s) 325, 448, 601, 789
Sau96I GGNCC 2 cut(s) 227, 489
SchI GAGTC 2 cut(s) 134, 457
ScrFI CCNGG 4 cut(s) 487, 488, 816, 1067
SduI GDGCHC 3 cut(s) 120, 658, 971
SexAI ACCWGGT 1 cut(s) 814
SfaNI GCATC 3 cut(s) 362, 899, 988
SfcI CTRYAG 4 cut(s) 62, 357, 441, 1014
SinI GGWCC 1 cut(s) 227
SmaI CCCGGG 1 cut(s) 488
SrfI GCCCGGGC 1 cut(s) 488
Sse9I AATT 8 cut(s) 278, 297, 346, 544, 593, 939, 1032, 1117
SsiI CCGC 3 cut(s) 120, 225, 363
SspMI CTAG 4 cut(s) 284, 636, 876, 1062
StyD4I CCNGG 4 cut(s) 485, 486, 814, 1065
StyI CCWWGG 2 cut(s) 186, 258
TaaI ACNGT 2 cut(s) 445, 966
TaiI ACGT 2 cut(s) 747, 990
TaqI TCGA 4 cut(s) 213, 658, 821, 847
TasI AATT 8 cut(s) 278, 297, 346, 544, 593, 939, 1032, 1117
TauI GCSGC 1 cut(s) 365
TfiI GAWTC 5 cut(s) 210, 369, 574, 888, 901
Tru1I TTAA 4 cut(s) 296, 801, 1031, 1104
Tru9I TTAA 4 cut(s) 296, 801, 1031, 1104
TscAI CASTG 3 cut(s) 388, 898, 1057
TseFI GTSAC 2 cut(s) 865, 977
TseI GCWGC 6 cut(s) 61, 359, 525, 732, 1016, 1090
Tsp45I GTSAC 2 cut(s) 865, 977
TspDTI ATGAA 7 cut(s) 43, 118, 168, 282, 326, 817, 1094
TspGWI ACGGA 1 cut(s) 473
TspMI CCCGGG 1 cut(s) 486
TspRI CASTG 3 cut(s) 388, 898, 1057
VpaK11BI GGWCC 1 cut(s) 227
XceI RCATGY 1 cut(s) 948
XcmI CCANNNNNNNNNTGG 1 cut(s) 722
XmaI CCCGGG 1 cut(s) 486
XmnI GAANNNNTTC 1 cut(s) 1046
XspI CTAG 4 cut(s) 284, 636, 876, 1062
ZraI GACGTC 1 cut(s) 745
Zsp2I ATGCAT 2 cut(s) 355, 1003
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.