MD10G1100600.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0009185
Physical Location & Seq
Forward (+)
16310490 .. 16312249
1760 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1100600.v1.1.491

Sequence Viewer

Length: 792 bp
ATGGCTAGGCACCTTCCAAGTAGCAGTATTAGGTTGTTCTCTTTGGTTATTTTGGTGATGGTGGTACTGGAAGCTGGAGAAGTTTGGTGCCTAGACGTGAACTTATCATCACCGTCAGCCGGTGGCGGTGGCGGTGGCGGTGGGAGGAGATTGTTGTCAGAAACGGTGCCTGCCATGTTTATATTTGGAGACTCTCTAATTGACAATGGGAATAACAACGACCTTCCGTCATTTGCCAAGGCTAACTATTTCCCTTATGGCATTGATTTCAATGGCGGTCCTACTGGCCGTTTTTCTAATGGTTACACCATGGTTGATGAAATAGCTGAACTGCTAGGACTTCCCTTGATTCCTGCTCACTCTGAAGCTTCTGGTGATCAAATGCGCCATGGAGTCAATTATGCATCTGCAGCTGCTGGAATCCTTGATGACACTGGCGGAAACTTTGTGGGAAGAATACCTTTTAGTGAACAAATAAGGAACTTTCAGAATACACTTGATCAGATAACAGACAATCTTGGTGCTGATGATGCTGCTCGATCGATTGCCAAGTGCATCTACTTCAATGGGTTGCATTCCAAGTATTTTGGCCCAAATCCAAGTGGAACCTGCTCGGATGAAGTGAATCGCCTTGTTCTACCTTTTAATACAAATGTGAAGACAATGATCAACAATCTTAATACCAACTTACCAGGCTCAAAGTTCATTTACGTAGACATTGCTCGTATGTTCGAAGACATGCTCCTCAATTCTAGATCCTATGGTATGCTTGCTACTGTATATTTAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

264

Amino Acids

28.31

Weight (kDa)

4.91

Isoelectric Point (pI)

31.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 60 - 172 5.3e-08 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 617
AccB1I GGYRCC 3 cut(s) 9, 87, 166
AccI GTMKAC 1 cut(s) 714
AciI CCGC 5 cut(s) 126, 132, 138, 276, 438
AclWI GGATC 1 cut(s) 750
AcoI YGGCCR 1 cut(s) 286
AcuI CTGAAG 1 cut(s) 384
AfaI GTAC 1 cut(s) 66
AfiI CCNNNNNNNGG 1 cut(s) 119
AgsI TTSAA 2 cut(s) 271, 565
AhdI GACNNNNNGTC 1 cut(s) 226
AjiI CACGTC 1 cut(s) 97
AjnI CCWGG 1 cut(s) 691
AluBI AGCT 5 cut(s) 74, 326, 368, 413, 789
AluI AGCT 5 cut(s) 74, 326, 368, 413, 789
Alw26I GTCTC 1 cut(s) 183
AlwI GGATC 1 cut(s) 750
AlwNI CAGNNNCTG 1 cut(s) 416
AoxI GGCC 2 cut(s) 286, 589
ApeKI GCWGC 3 cut(s) 410, 413, 533
AspLEI GCGC 1 cut(s) 387
AspS9I GGNCC 2 cut(s) 278, 590
AsuHPI GGTGA 3 cut(s) 67, 102, 386
AsuII TTCGAA 1 cut(s) 732
AvaII GGWCC 1 cut(s) 278
BanI GGYRCC 3 cut(s) 9, 87, 166
BbsI GAAGAC 2 cut(s) 665, 741
BbvI GCAGC 3 cut(s) 400, 422, 520
BccI CCATC 1 cut(s) 52
BceAI ACGGC 1 cut(s) 273
BciT130I CCWGG 1 cut(s) 693
BclI TGATCA 3 cut(s) 376, 499, 666
BcoDI GTCTC 1 cut(s) 183
BfaI CTAG 4 cut(s) 6, 92, 335, 753
BfmI CTRYAG 1 cut(s) 408
BfuAI ACCTGC 1 cut(s) 617
BisI GCNGC 3 cut(s) 411, 414, 534
BlsI GCNGC 3 cut(s) 412, 415, 535
Bme1390I CCNGG 1 cut(s) 693
Bme18I GGWCC 1 cut(s) 278
BmeRI GACNNNNNGTC 1 cut(s) 226
BmgBI CACGTC 1 cut(s) 97
BmgT120I GGNCC 2 cut(s) 278, 590
BmiI GGNNCC 4 cut(s) 11, 89, 168, 607
BmrFI CCNGG 1 cut(s) 693
BmsI GCATC 3 cut(s) 413, 520, 564
BpiI GAAGAC 2 cut(s) 665, 741
BpmI CTGGAG 1 cut(s) 96
Bpu14I TTCGAA 1 cut(s) 732
Bsa29I ATCGAT 1 cut(s) 542
BsaAI YACGTR 1 cut(s) 712
BsaJI CCNNGG 3 cut(s) 237, 309, 388
Bsc4I CCNNNNNNNGG 1 cut(s) 119
Bse118I RCCGGY 1 cut(s) 119
Bse1I ACTGG 3 cut(s) 72, 289, 439
Bse3DI GCAATG 1 cut(s) 717
BseBI CCWGG 1 cut(s) 693
BseCI ATCGAT 1 cut(s) 542
BseDI CCNNGG 3 cut(s) 237, 309, 388
BseGI GGATG 1 cut(s) 622
BseLI CCNNNNNNNGG 1 cut(s) 119
BseMI GCAATG 1 cut(s) 717
BseNI ACTGG 3 cut(s) 72, 289, 439
BseRI GAGGAG 2 cut(s) 160, 734
BseXI GCAGC 3 cut(s) 400, 422, 520
Bsh1285I CGRYCG 1 cut(s) 542
BshFI GGCC 2 cut(s) 288, 591
BshNI GGYRCC 3 cut(s) 9, 87, 166
BshVI ATCGAT 1 cut(s) 542
BsiEI CGRYCG 1 cut(s) 542
BsiSI CCGG 1 cut(s) 120
BslI CCNNNNNNNGG 1 cut(s) 119
BsmAI GTCTC 1 cut(s) 183
BsmI GAATGC 1 cut(s) 574
BsnI GGCC 2 cut(s) 288, 591
Bsp119I TTCGAA 1 cut(s) 732
Bsp143I GATC 5 cut(s) 376, 499, 539, 666, 755
Bsp19I CCATGG 2 cut(s) 309, 388
BspACI CCGC 5 cut(s) 126, 132, 138, 276, 438
BspANI GGCC 2 cut(s) 288, 591
BspDI ATCGAT 1 cut(s) 542
BspLI GGNNCC 4 cut(s) 11, 89, 168, 607
BspMAI CTGCAG 1 cut(s) 412
BspMI ACCTGC 1 cut(s) 617
BspPI GGATC 1 cut(s) 750
BspT104I TTCGAA 1 cut(s) 732
BspT107I GGYRCC 3 cut(s) 9, 87, 166
BsrDI GCAATG 1 cut(s) 717
BsrFI RCCGGY 1 cut(s) 119
BsrI ACTGG 3 cut(s) 72, 289, 439
BssAI RCCGGY 1 cut(s) 119
BssECI CCNNGG 3 cut(s) 237, 309, 388
BssMI GATC 5 cut(s) 376, 499, 539, 666, 755
BssT1I CCWWGG 3 cut(s) 237, 309, 388
Bst2UI CCWGG 1 cut(s) 693
Bst4CI ACNGT 3 cut(s) 114, 166, 778
BstBAI YACGTR 1 cut(s) 712
BstBI TTCGAA 1 cut(s) 732
BstC8I GCNNGC 2 cut(s) 171, 771
BstDSI CCRYGG 2 cut(s) 309, 388
BstF5I GGATG 1 cut(s) 622
BstHHI GCGC 1 cut(s) 387
BstKTI GATC 5 cut(s) 379, 502, 542, 669, 758
BstMAI GTCTC 1 cut(s) 183
BstMBI GATC 5 cut(s) 376, 499, 539, 666, 755
BstMCI CGRYCG 1 cut(s) 542
BstMWI GCNNNNNNNGC 2 cut(s) 410, 530
BstNI CCWGG 1 cut(s) 693
BstNSI RCATGY 1 cut(s) 742
BstSCI CCNGG 1 cut(s) 691
BstSFI CTRYAG 1 cut(s) 408
BstSNI TACGTA 1 cut(s) 712
BstV1I GCAGC 3 cut(s) 400, 422, 520
BstV2I GAAGAC 2 cut(s) 665, 741
BstX2I RGATCY 1 cut(s) 755
BstYI RGATCY 1 cut(s) 755
Bsu15I ATCGAT 1 cut(s) 542
BsuRI GGCC 2 cut(s) 288, 591
BsuTUI ATCGAT 1 cut(s) 542
BtgI CCRYGG 2 cut(s) 309, 388
BtrI CACGTC 1 cut(s) 97
BtsCI GGATG 1 cut(s) 622
BtsIMutI CAGTG 1 cut(s) 432
BveI ACCTGC 1 cut(s) 617
Cac8I GCNNGC 2 cut(s) 171, 771
CaiI CAGNNNCTG 1 cut(s) 416
CfoI GCGC 1 cut(s) 387
Cfr10I RCCGGY 1 cut(s) 119
Cfr13I GGNCC 2 cut(s) 278, 590
ClaI ATCGAT 1 cut(s) 542
Csp6I GTAC 1 cut(s) 65
CviAII CATG 4 cut(s) 175, 310, 389, 739
CviQI GTAC 1 cut(s) 65
DpnI GATC 5 cut(s) 378, 501, 541, 668, 757
DpnII GATC 5 cut(s) 376, 499, 539, 666, 755
DriI GACNNNNNGTC 1 cut(s) 226
EaeI YGGCCR 1 cut(s) 286
Eam1105I GACNNNNNGTC 1 cut(s) 226
EciI GGCGGA 1 cut(s) 453
Eco105I TACGTA 1 cut(s) 712
Eco130I CCWWGG 3 cut(s) 237, 309, 388
Eco47I GGWCC 1 cut(s) 278
Eco57I CTGAAG 1 cut(s) 384
EcoRII CCWGG 1 cut(s) 691
EcoT14I CCWWGG 3 cut(s) 237, 309, 388
EcoT22I ATGCAT 1 cut(s) 406
ErhI CCWWGG 3 cut(s) 237, 309, 388
FaeI CATG 4 cut(s) 178, 313, 392, 742
FalI AAGNNNNNCTT 2 cut(s) 445, 477
FatI CATG 4 cut(s) 174, 309, 388, 738
FbaI TGATCA 3 cut(s) 376, 499, 666
FblI GTMKAC 1 cut(s) 714
Fnu4HI GCNGC 3 cut(s) 411, 414, 534
FokI GGATG 1 cut(s) 629
Fsp4HI GCNGC 3 cut(s) 411, 414, 534
FspBI CTAG 4 cut(s) 6, 92, 335, 753
GlaI GCGC 1 cut(s) 386
GluI GCNGC 3 cut(s) 411, 414, 534
GsuI CTGGAG 1 cut(s) 96
HaeIII GGCC 2 cut(s) 288, 591
HapII CCGG 1 cut(s) 120
HhaI GCGC 1 cut(s) 387
Hin1II CATG 4 cut(s) 178, 313, 392, 742
Hin6I GCGC 1 cut(s) 385
HinP1I GCGC 1 cut(s) 385
HindIII AAGCTT 1 cut(s) 366
HinfI GANTC 5 cut(s) 191, 349, 393, 420, 625
HpaII CCGG 1 cut(s) 120
HphI GGTGA 3 cut(s) 67, 102, 386
Hpy166II GTNNAC 3 cut(s) 100, 470, 715
Hpy188I TCNGA 5 cut(s) 160, 364, 489, 504, 616
Hpy188III TCNNGA 1 cut(s) 753
Hpy8I GTNNAC 3 cut(s) 100, 470, 715
HpyAV CCTTC 2 cut(s) 23, 233
HpyCH4III ACNGT 3 cut(s) 114, 166, 778
HpyCH4IV ACGT 2 cut(s) 96, 711
HpyCH4V TGCA 4 cut(s) 404, 410, 555, 574
HpyF10VI GCNNNNNNNGC 2 cut(s) 410, 530
HpySE526I ACGT 2 cut(s) 96, 711
Hsp92II CATG 4 cut(s) 178, 313, 392, 742
HspAI GCGC 1 cut(s) 385
Ksp22I TGATCA 3 cut(s) 376, 499, 666
Kzo9I GATC 5 cut(s) 376, 499, 539, 666, 755
LmnI GCTCC 1 cut(s) 747
Lsp1109I GCAGC 3 cut(s) 400, 422, 520
LweI GCATC 3 cut(s) 413, 520, 564
MaeI CTAG 4 cut(s) 6, 92, 335, 753
MaeII ACGT 2 cut(s) 96, 711
MaeIII GTNAC 1 cut(s) 302
MalI GATC 5 cut(s) 378, 501, 541, 668, 757
MboI GATC 5 cut(s) 376, 499, 539, 666, 755
MboII GAAGA 3 cut(s) 465, 670, 746
MflI RGATCY 1 cut(s) 755
MluCI AATT 3 cut(s) 198, 397, 748
MlyI GAGTC 2 cut(s) 185, 402
MnlI CCTC 2 cut(s) 138, 755
Mph1103I ATGCAT 1 cut(s) 406
MseI TTAA 3 cut(s) 645, 678, 785
MspA1I CMGCKG 1 cut(s) 413
MspI CCGG 1 cut(s) 120
MspR9I CCNGG 1 cut(s) 693
Mva1269I GAATGC 1 cut(s) 574
MvaI CCWGG 1 cut(s) 693
MwoI GCNNNNNNNGC 2 cut(s) 410, 530
NcoI CCATGG 2 cut(s) 309, 388
NdeII GATC 5 cut(s) 376, 499, 539, 666, 755
NlaIII CATG 4 cut(s) 178, 313, 392, 742
NlaIV GGNNCC 4 cut(s) 11, 89, 168, 607
NsiI ATGCAT 1 cut(s) 406
NspI RCATGY 1 cut(s) 742
NspV TTCGAA 1 cut(s) 732
PctI GAATGC 1 cut(s) 574
PfeI GAWTC 3 cut(s) 349, 420, 625
PkrI GCNGC 3 cut(s) 412, 415, 535
Ple19I CGATCG 1 cut(s) 542
PleI GAGTC 2 cut(s) 185, 401
PpsI GAGTC 2 cut(s) 185, 401
Ppu21I YACGTR 1 cut(s) 712
Psp6I CCWGG 1 cut(s) 691
PspGI CCWGG 1 cut(s) 691
PspN4I GGNNCC 4 cut(s) 11, 89, 168, 607
PspPI GGNCC 2 cut(s) 278, 590
PstI CTGCAG 1 cut(s) 412
PstNI CAGNNNCTG 1 cut(s) 416
PsuI RGATCY 1 cut(s) 755
PvuI CGATCG 1 cut(s) 542
PvuII CAGCTG 1 cut(s) 413
RsaI GTAC 1 cut(s) 66
RsaNI GTAC 1 cut(s) 65
SaqAI TTAA 3 cut(s) 645, 678, 785
SatI GCNGC 3 cut(s) 411, 414, 534
Sau3AI GATC 5 cut(s) 376, 499, 539, 666, 755
Sau96I GGNCC 2 cut(s) 278, 590
SchI GAGTC 2 cut(s) 185, 402
ScrFI CCNGG 1 cut(s) 693
SfaNI GCATC 3 cut(s) 413, 520, 564
SfcI CTRYAG 1 cut(s) 408
SfuI TTCGAA 1 cut(s) 732
SinI GGWCC 1 cut(s) 278
SnaBI TACGTA 1 cut(s) 712
Sse9I AATT 3 cut(s) 198, 397, 748
SsiI CCGC 5 cut(s) 126, 132, 138, 276, 438
SspMI CTAG 4 cut(s) 6, 92, 335, 753
StyD4I CCNGG 1 cut(s) 691
StyI CCWWGG 3 cut(s) 237, 309, 388
TaaI ACNGT 3 cut(s) 114, 166, 778
TaiI ACGT 2 cut(s) 99, 714
TaqI TCGA 3 cut(s) 538, 542, 732
TasI AATT 3 cut(s) 198, 397, 748
TfiI GAWTC 3 cut(s) 349, 420, 625
Tru1I TTAA 3 cut(s) 645, 678, 785
Tru9I TTAA 3 cut(s) 645, 678, 785
TscAI CASTG 1 cut(s) 439
TseI GCWGC 3 cut(s) 410, 413, 533
TspDTI ATGAA 3 cut(s) 333, 633, 694
TspGWI ACGGA 1 cut(s) 216
TspRI CASTG 1 cut(s) 439
VpaK11BI GGWCC 1 cut(s) 278
XbaI TCTAGA 1 cut(s) 752
XceI RCATGY 1 cut(s) 742
XmiI GTMKAC 1 cut(s) 714
XspI CTAG 4 cut(s) 6, 92, 335, 753
Zsp2I ATGCAT 1 cut(s) 406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.