MD15G1400200.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0009185
Physical Location & Seq
Reverse (-)
50069868 .. 50072273
2406 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1400200.v1.1.491

Sequence Viewer

Length: 1176 bp
ATGGCTAGGCACCTTCCAAGTAGCAGTATTAGGTTGTTCTCTTTGGTTATTTTGGTGATGGTGGTACTGGAAGCTGGAGAAGTTTGGTGCCTAGACGTGAACTTATCATCACCGTCAGCCGGTGGCGGTGGCGGTGGCGGTGGGAGGAGATTGTTGTCAGAAACGGTGCCTGCCATGTTTATATTTGGAGACTCTCTAATTGACAATGGGAATAACAACGACCTTCCGTCATTTGCCAAGGCTAACTATTTCCCTTATGGCATTGATTTCAATGGCGGTCCTACTGGCCGTTTTTCTAATGGTTACACCATGGTTGATGAAATAGCTGAACTGCTAGGACTTCCCTTGATTCCTGCTCACTCTGAAGCTTCTGGTGATCAAATGCGCCATGGAGTCAATTATGCATCTGCAGCTGCTGGAATCCTTGATGACACTGGCGGAAACTTTGTGGGAAGAATACCTTTTAGTGAACAAATAAGGAACTTTCAGAATACACTTGATCAGATAACAGACAATCTTGGTGCTGATGATGCTGCTCGATCGATTGCCAAGTGCATCTACTTCGTTGGAATGGGCAGCAATGACTACCTAAACAATTACCTTATGCCCAACTATCAAACTAGGAATCAGTACAATGCCCAACAATATGCTGATCTCTTGGCTCAAGAATATACACAGCAGCTCACTAGACTTTACAATCTTGGTGCCCGAAAATTTGTCATTGCTGGATTAGGGAGAATGGGTTGCATTCCAAGTATTTTGGCCCAAAATCCAAGTGGAACCTGCTCGGATGAAGTGAATCGCCTTGTTCTACCTTTTAATACAAATGTGAAGACAATGATCAACAATCTTAATACCAACTTACCAGGCTCAAAGTTCATTTACGTAGACATTGCTCGTATGTTCGAAGACATGCTCCTCAATTCTAGATCCTATGGATTCAGCATTGCAAATCGTGGATGCTGCGGTATTGGGCGTAACAGAGGCCAAGTTACATGTCTTCCTTTCCAAACACCATGCCCAAATCGAGACCAATATATCTTCTGGGATGCATTCCACCCGACATCAGCGGTGAACATCATAATTGGGAGGAAAGCTTTCAGCGGGGACCGTACCGAGGTTTATCCCATGAACATACAGCAGCTTGCCACTCTTGACATTGAAAGCAACCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

392

Amino Acids

42.79

Weight (kDa)

5.29

Isoelectric Point (pI)

33.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 60 - 364 5.1e-37 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 791
AccB1I GGYRCC 4 cut(s) 9, 87, 166, 704
AccI GTMKAC 1 cut(s) 888
AciI CCGC 8 cut(s) 126, 132, 138, 276, 438, 966, 1070, 1104
AclWI GGATC 1 cut(s) 924
AcoI YGGCCR 1 cut(s) 286
AcsI RAATTY 1 cut(s) 713
AcuI CTGAAG 1 cut(s) 384
AfaI GTAC 3 cut(s) 66, 632, 1114
AfiI CCNNNNNNNGG 2 cut(s) 119, 1117
AflIII ACRYGT 1 cut(s) 995
AgsI TTSAA 2 cut(s) 271, 1163
AhdI GACNNNNNGTC 1 cut(s) 226
AjiI CACGTC 1 cut(s) 97
AjnI CCWGG 1 cut(s) 865
AluBI AGCT 7 cut(s) 74, 326, 368, 413, 682, 1097, 1144
AluI AGCT 7 cut(s) 74, 326, 368, 413, 682, 1097, 1144
Alw26I GTCTC 2 cut(s) 183, 1023
AlwI GGATC 1 cut(s) 924
AlwNI CAGNNNCTG 1 cut(s) 416
AoxI GGCC 3 cut(s) 286, 762, 985
ApeKI GCWGC 7 cut(s) 410, 413, 533, 576, 679, 963, 1141
ApoI RAATTY 1 cut(s) 713
Asp700I GAANNNNTTC 1 cut(s) 1097
AspLEI GCGC 1 cut(s) 387
AspS9I GGNCC 3 cut(s) 278, 763, 1108
AsuHPI GGTGA 4 cut(s) 67, 102, 386, 1084
AsuII TTCGAA 1 cut(s) 906
AvaII GGWCC 2 cut(s) 278, 1108
BaeGI GKGCMC 1 cut(s) 709
BanI GGYRCC 4 cut(s) 9, 87, 166, 704
BbsI GAAGAC 3 cut(s) 839, 915, 992
BbvI GCAGC 7 cut(s) 400, 422, 520, 588, 691, 950, 1153
BccI CCATC 1 cut(s) 52
BceAI ACGGC 1 cut(s) 273
BcgI CGANNNNNNTGC 2 cut(s) 544, 578
BciT130I CCWGG 1 cut(s) 867
BclI TGATCA 3 cut(s) 376, 499, 840
BcoDI GTCTC 2 cut(s) 183, 1023
BfaI CTAG 6 cut(s) 6, 92, 335, 621, 687, 927
BfmI CTRYAG 1 cut(s) 408
BfuAI ACCTGC 1 cut(s) 791
BisI GCNGC 7 cut(s) 411, 414, 534, 577, 680, 964, 1142
BlsI GCNGC 7 cut(s) 412, 415, 535, 578, 681, 965, 1143
Bme1390I CCNGG 1 cut(s) 867
Bme18I GGWCC 2 cut(s) 278, 1108
BmeRI GACNNNNNGTC 1 cut(s) 226
BmgBI CACGTC 1 cut(s) 97
BmgT120I GGNCC 3 cut(s) 278, 763, 1108
BmiI GGNNCC 6 cut(s) 11, 89, 168, 706, 781, 1109
BmrFI CCNGG 1 cut(s) 867
BmsI GCATC 5 cut(s) 413, 520, 564, 950, 1039
BpiI GAAGAC 3 cut(s) 839, 915, 992
BpmI CTGGAG 1 cut(s) 96
Bpu14I TTCGAA 1 cut(s) 906
BpuEI CTTGAG 1 cut(s) 648
Bsa29I ATCGAT 1 cut(s) 542
BsaAI YACGTR 1 cut(s) 886
BsaI GGTCTC 1 cut(s) 1023
BsaJI CCNNGG 4 cut(s) 237, 309, 388, 1116
BsaXI ACNNNNNCTCC 2 cut(s) 727, 757
Bsc4I CCNNNNNNNGG 2 cut(s) 119, 1117
Bse118I RCCGGY 1 cut(s) 119
Bse1I ACTGG 4 cut(s) 72, 289, 439, 1171
Bse3DI GCAATG 4 cut(s) 586, 720, 891, 945
BseBI CCWGG 1 cut(s) 867
BseCI ATCGAT 1 cut(s) 542
BseDI CCNNGG 4 cut(s) 237, 309, 388, 1116
BseGI GGATG 3 cut(s) 796, 965, 1054
BseLI CCNNNNNNNGG 2 cut(s) 119, 1117
BseMI GCAATG 4 cut(s) 586, 720, 891, 945
BseNI ACTGG 4 cut(s) 72, 289, 439, 1171
BseRI GAGGAG 2 cut(s) 160, 908
BseSI GKGCMC 1 cut(s) 709
BseXI GCAGC 7 cut(s) 400, 422, 520, 588, 691, 950, 1153
Bsh1285I CGRYCG 1 cut(s) 542
BshFI GGCC 3 cut(s) 288, 764, 987
BshNI GGYRCC 4 cut(s) 9, 87, 166, 704
BshVI ATCGAT 1 cut(s) 542
BsiEI CGRYCG 1 cut(s) 542
BsiSI CCGG 1 cut(s) 120
BslFI GGGAC 1 cut(s) 1121
BslI CCNNNNNNNGG 2 cut(s) 119, 1117
BsmAI GTCTC 2 cut(s) 183, 1023
BsmFI GGGAC 1 cut(s) 1121
BsmI GAATGC 2 cut(s) 747, 1052
BsnI GGCC 3 cut(s) 288, 764, 987
Bso31I GGTCTC 1 cut(s) 1023
Bsp119I TTCGAA 1 cut(s) 906
Bsp1286I GDGCHC 1 cut(s) 709
Bsp143I GATC 6 cut(s) 376, 499, 539, 652, 840, 929
Bsp19I CCATGG 2 cut(s) 309, 388
BspACI CCGC 8 cut(s) 126, 132, 138, 276, 438, 966, 1070, 1104
BspANI GGCC 3 cut(s) 288, 764, 987
BspDI ATCGAT 1 cut(s) 542
BspLI GGNNCC 6 cut(s) 11, 89, 168, 706, 781, 1109
BspMAI CTGCAG 1 cut(s) 412
BspMI ACCTGC 1 cut(s) 791
BspPI GGATC 1 cut(s) 924
BspT104I TTCGAA 1 cut(s) 906
BspT107I GGYRCC 4 cut(s) 9, 87, 166, 704
BspTNI GGTCTC 1 cut(s) 1023
BsrDI GCAATG 4 cut(s) 586, 720, 891, 945
BsrFI RCCGGY 1 cut(s) 119
BsrI ACTGG 4 cut(s) 72, 289, 439, 1171
BssAI RCCGGY 1 cut(s) 119
BssECI CCNNGG 4 cut(s) 237, 309, 388, 1116
BssMI GATC 6 cut(s) 376, 499, 539, 652, 840, 929
BssT1I CCWWGG 3 cut(s) 237, 309, 388
Bst2UI CCWGG 1 cut(s) 867
Bst4CI ACNGT 3 cut(s) 114, 166, 1112
BstBAI YACGTR 1 cut(s) 886
BstBI TTCGAA 1 cut(s) 906
BstC8I GCNNGC 2 cut(s) 171, 1146
BstDSI CCRYGG 2 cut(s) 309, 388
BstF5I GGATG 3 cut(s) 796, 965, 1054
BstHHI GCGC 1 cut(s) 387
BstKTI GATC 6 cut(s) 379, 502, 542, 655, 843, 932
BstMAI GTCTC 2 cut(s) 183, 1023
BstMBI GATC 6 cut(s) 376, 499, 539, 652, 840, 929
BstMCI CGRYCG 1 cut(s) 542
BstMWI GCNNNNNNNGC 2 cut(s) 410, 530
BstNI CCWGG 1 cut(s) 867
BstNSI RCATGY 2 cut(s) 916, 999
BstSCI CCNGG 1 cut(s) 865
BstSFI CTRYAG 1 cut(s) 408
BstSLI GKGCMC 1 cut(s) 709
BstSNI TACGTA 1 cut(s) 886
BstV1I GCAGC 7 cut(s) 400, 422, 520, 588, 691, 950, 1153
BstV2I GAAGAC 3 cut(s) 839, 915, 992
BstX2I RGATCY 1 cut(s) 929
BstYI RGATCY 1 cut(s) 929
Bsu15I ATCGAT 1 cut(s) 542
BsuRI GGCC 3 cut(s) 288, 764, 987
BsuTUI ATCGAT 1 cut(s) 542
BtgI CCRYGG 2 cut(s) 309, 388
BtrI CACGTC 1 cut(s) 97
BtsCI GGATG 3 cut(s) 796, 965, 1054
BtsIMutI CAGTG 1 cut(s) 432
BveI ACCTGC 1 cut(s) 791
Cac8I GCNNGC 2 cut(s) 171, 1146
CaiI CAGNNNCTG 1 cut(s) 416
CfoI GCGC 1 cut(s) 387
Cfr10I RCCGGY 1 cut(s) 119
Cfr13I GGNCC 3 cut(s) 278, 763, 1108
ClaI ATCGAT 1 cut(s) 542
Csp6I GTAC 3 cut(s) 65, 631, 1113
CviAII CATG 7 cut(s) 175, 310, 389, 913, 996, 1017, 1129
CviQI GTAC 3 cut(s) 65, 631, 1113
DpnI GATC 6 cut(s) 378, 501, 541, 654, 842, 931
DpnII GATC 6 cut(s) 376, 499, 539, 652, 840, 929
DriI GACNNNNNGTC 1 cut(s) 226
EaeI YGGCCR 1 cut(s) 286
Eam1105I GACNNNNNGTC 1 cut(s) 226
EciI GGCGGA 1 cut(s) 453
Eco105I TACGTA 1 cut(s) 886
Eco130I CCWWGG 3 cut(s) 237, 309, 388
Eco31I GGTCTC 1 cut(s) 1023
Eco47I GGWCC 2 cut(s) 278, 1108
Eco57I CTGAAG 1 cut(s) 384
EcoRII CCWGG 1 cut(s) 865
EcoT14I CCWWGG 3 cut(s) 237, 309, 388
EcoT22I ATGCAT 2 cut(s) 406, 1054
ErhI CCWWGG 3 cut(s) 237, 309, 388
FaeI CATG 7 cut(s) 178, 313, 392, 916, 999, 1020, 1132
FalI AAGNNNNNCTT 2 cut(s) 445, 477
FaqI GGGAC 1 cut(s) 1121
FatI CATG 7 cut(s) 174, 309, 388, 912, 995, 1016, 1128
FauI CCCGC 1 cut(s) 1097
FbaI TGATCA 3 cut(s) 376, 499, 840
FblI GTMKAC 1 cut(s) 888
Fnu4HI GCNGC 7 cut(s) 411, 414, 534, 577, 680, 964, 1142
FokI GGATG 3 cut(s) 803, 972, 1061
Fsp4HI GCNGC 7 cut(s) 411, 414, 534, 577, 680, 964, 1142
FspBI CTAG 6 cut(s) 6, 92, 335, 621, 687, 927
GlaI GCGC 1 cut(s) 386
GluI GCNGC 7 cut(s) 411, 414, 534, 577, 680, 964, 1142
GsuI CTGGAG 1 cut(s) 96
HaeIII GGCC 3 cut(s) 288, 764, 987
HapII CCGG 1 cut(s) 120
HhaI GCGC 1 cut(s) 387
Hin1II CATG 7 cut(s) 178, 313, 392, 916, 999, 1020, 1132
Hin6I GCGC 1 cut(s) 385
HinP1I GCGC 1 cut(s) 385
HindIII AAGCTT 2 cut(s) 366, 1095
HinfI GANTC 7 cut(s) 191, 349, 393, 420, 625, 799, 939
HpaII CCGG 1 cut(s) 120
HphI GGTGA 4 cut(s) 67, 102, 386, 1084
Hpy166II GTNNAC 4 cut(s) 100, 470, 889, 1075
Hpy188I TCNGA 5 cut(s) 160, 364, 489, 504, 790
Hpy188III TCNNGA 4 cut(s) 665, 927, 1028, 1154
Hpy8I GTNNAC 4 cut(s) 100, 470, 889, 1075
HpyAV CCTTC 2 cut(s) 23, 233
HpyCH4III ACNGT 3 cut(s) 114, 166, 1112
HpyCH4IV ACGT 2 cut(s) 96, 885
HpyCH4V TGCA 6 cut(s) 404, 410, 555, 747, 950, 1052
HpyF10VI GCNNNNNNNGC 2 cut(s) 410, 530
HpySE526I ACGT 2 cut(s) 96, 885
Hsp92II CATG 7 cut(s) 178, 313, 392, 916, 999, 1020, 1132
HspAI GCGC 1 cut(s) 385
Ksp22I TGATCA 3 cut(s) 376, 499, 840
Kzo9I GATC 6 cut(s) 376, 499, 539, 652, 840, 929
LmnI GCTCC 1 cut(s) 921
Lsp1109I GCAGC 7 cut(s) 400, 422, 520, 588, 691, 950, 1153
LweI GCATC 5 cut(s) 413, 520, 564, 950, 1039
MaeI CTAG 6 cut(s) 6, 92, 335, 621, 687, 927
MaeII ACGT 2 cut(s) 96, 885
MaeIII GTNAC 3 cut(s) 302, 977, 991
MalI GATC 6 cut(s) 378, 501, 541, 654, 842, 931
MboI GATC 6 cut(s) 376, 499, 539, 652, 840, 929
MboII GAAGA 5 cut(s) 465, 844, 920, 992, 1033
MflI RGATCY 1 cut(s) 929
MhlI GDGCHC 1 cut(s) 709
MluCI AATT 6 cut(s) 198, 397, 595, 713, 922, 1083
MlyI GAGTC 2 cut(s) 185, 402
MmeI TCCRAC 1 cut(s) 547
MnlI CCTC 5 cut(s) 138, 929, 977, 1083, 1111
Mph1103I ATGCAT 2 cut(s) 406, 1054
MroXI GAANNNNTTC 1 cut(s) 1097
MseI TTAA 2 cut(s) 819, 852
MspA1I CMGCKG 3 cut(s) 413, 1070, 1104
MspI CCGG 1 cut(s) 120
MspR9I CCNGG 1 cut(s) 867
Mva1269I GAATGC 2 cut(s) 747, 1052
MvaI CCWGG 1 cut(s) 867
MwoI GCNNNNNNNGC 2 cut(s) 410, 530
NcoI CCATGG 2 cut(s) 309, 388
NdeII GATC 6 cut(s) 376, 499, 539, 652, 840, 929
NlaIII CATG 7 cut(s) 178, 313, 392, 916, 999, 1020, 1132
NlaIV GGNNCC 6 cut(s) 11, 89, 168, 706, 781, 1109
NsiI ATGCAT 2 cut(s) 406, 1054
NspI RCATGY 2 cut(s) 916, 999
NspV TTCGAA 1 cut(s) 906
PciI ACATGT 1 cut(s) 995
PctI GAATGC 2 cut(s) 747, 1052
PdmI GAANNNNTTC 1 cut(s) 1097
PfeI GAWTC 5 cut(s) 349, 420, 625, 799, 939
PkrI GCNGC 7 cut(s) 412, 415, 535, 578, 681, 965, 1143
Ple19I CGATCG 1 cut(s) 542
PleI GAGTC 2 cut(s) 185, 401
PpsI GAGTC 2 cut(s) 185, 401
Ppu21I YACGTR 1 cut(s) 886
PscI ACATGT 1 cut(s) 995
Psp6I CCWGG 1 cut(s) 865
PspGI CCWGG 1 cut(s) 865
PspN4I GGNNCC 6 cut(s) 11, 89, 168, 706, 781, 1109
PspPI GGNCC 3 cut(s) 278, 763, 1108
PstI CTGCAG 1 cut(s) 412
PstNI CAGNNNCTG 1 cut(s) 416
PsuI RGATCY 1 cut(s) 929
PvuI CGATCG 1 cut(s) 542
PvuII CAGCTG 1 cut(s) 413
RsaI GTAC 3 cut(s) 66, 632, 1114
RsaNI GTAC 3 cut(s) 65, 631, 1113
SaqAI TTAA 2 cut(s) 819, 852
SatI GCNGC 7 cut(s) 411, 414, 534, 577, 680, 964, 1142
Sau3AI GATC 6 cut(s) 376, 499, 539, 652, 840, 929
Sau96I GGNCC 3 cut(s) 278, 763, 1108
SchI GAGTC 2 cut(s) 185, 402
ScrFI CCNGG 1 cut(s) 867
SduI GDGCHC 1 cut(s) 709
SfaNI GCATC 5 cut(s) 413, 520, 564, 950, 1039
SfcI CTRYAG 1 cut(s) 408
SfuI TTCGAA 1 cut(s) 906
SinI GGWCC 2 cut(s) 278, 1108
SmlI CTYRAG 1 cut(s) 663
SmoI CTYRAG 1 cut(s) 663
SnaBI TACGTA 1 cut(s) 886
Sse9I AATT 6 cut(s) 198, 397, 595, 713, 922, 1083
SsiI CCGC 8 cut(s) 126, 132, 138, 276, 438, 966, 1070, 1104
SspMI CTAG 6 cut(s) 6, 92, 335, 621, 687, 927
StyD4I CCNGG 1 cut(s) 865
StyI CCWWGG 3 cut(s) 237, 309, 388
TaaI ACNGT 3 cut(s) 114, 166, 1112
TaiI ACGT 2 cut(s) 99, 888
TaqI TCGA 4 cut(s) 538, 542, 906, 1027
TasI AATT 6 cut(s) 198, 397, 595, 713, 922, 1083
TatI WGTACW 1 cut(s) 630
TfiI GAWTC 5 cut(s) 349, 420, 625, 799, 939
Tru1I TTAA 2 cut(s) 819, 852
Tru9I TTAA 2 cut(s) 819, 852
TscAI CASTG 1 cut(s) 439
TseI GCWGC 7 cut(s) 410, 413, 533, 576, 679, 963, 1141
TspDTI ATGAA 4 cut(s) 333, 807, 868, 1145
TspGWI ACGGA 1 cut(s) 216
TspRI CASTG 1 cut(s) 439
VpaK11BI GGWCC 2 cut(s) 278, 1108
XapI RAATTY 1 cut(s) 713
XbaI TCTAGA 1 cut(s) 926
XceI RCATGY 2 cut(s) 916, 999
XcmI CCANNNNNNNNNTGG 1 cut(s) 773
XmiI GTMKAC 1 cut(s) 888
XmnI GAANNNNTTC 1 cut(s) 1097
XspI CTAG 6 cut(s) 6, 92, 335, 621, 687, 927
Zsp2I ATGCAT 2 cut(s) 406, 1054
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.