pycom15g35880

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0009185
Physical Location & Seq
Reverse (-)
35948201 .. 35950239
2039 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g35880.1

Sequence Viewer

Length: 1170 bp
ATGGCTAGGCACCTTCCAAGTAGCAGTATTAGGTTGTTCTGTGTGGTTGTTTTGGTGATGGTGGTTCTGGAAGCTGGAGTAGTTTGGTGCCAACATGTGAACTCATCATCACCGTCAGCCGGTGGCGGTGGCGGTGGGAGGAGATTGTTGTCAGAAACGGTGCCTGCCATGTTTATATTTGGAGACTCTCTAATTGACAATGGGAATAACAACGACATTCCGTCATTTGCCAAGGCTAACTATTTCCCTTATGGCATTGATTTCAACGGCGGTCCTACTGGCCGTTTTTCCAATGGCTACACCATGGTTGATGAAATATCTGAACTGCTAGGACTTCCCTTGATTCCTGCTCACTCTGAAGCTTCTGGTGATCAAATGCGTCATGGAGTCAATTATGCATCTGCAGCTGCTGGAATCCTTGATGACACTGGCAGAAACTTCGTGGGAAGAATACCTTTTAGTGAACAAATAAGAAACTTTCAGAATACACTTGATCAGATAACAGGCAATCTTGGTGCTGATGATGCTGCTCGATCTATTGCCAAGTGCATCTTCTTCGTTGGAATGGGAAGCAATGACTACCTGAATAATTACCTAATGCCCAACTATCCAACTAGGAATCAGTACAATGCCCAACAATATGCTGATCTCTTGGCTCAAGAATATACACAGCAGCTCACTAGACTTTACAATCTTGGTGCCCGAAAATTTGTTATTGCTGGATTAGGAAGAATGGGTTGCATTCCAAGTATTTTGGCCCAAAATCCAAGTGGAACCTGCTCGGACGGAGTTAATCGCCTTGTTCTACCTTTTAATACAAATGTGAAGACAATGATCAACAATCTTAATGCCAACTTACCGAGCTCAAAGTTCATTTACATAGACATCGCTCGTATGTTCGAAGACATGCTCCTCAATTCTAGATCCTATGGATTCAGCGTTGCAAATCGTGGATGCTGCGGTATTGGGCGTAACAGAGGCCAAGTTACATGTCTTCCTTTCCAAACACCATGCCCAAATCGAGACCAATATATCTTCTGGGATGCATTCCACCCGACATCAGCGGTGAACATCATAATTGGGAGGAAAGCTTTCAGCGGGGACCGTACCGAGGTTTATCCCATGAACATACAGCAGCTTGCCACTCTTGACATTGAAAGGAACCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

390

Amino Acids

42.66

Weight (kDa)

6.94

Isoelectric Point (pI)

33.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 58 - 362 1.8e-35 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 785
AccB1I GGYRCC 4 cut(s) 9, 87, 160, 698
AciI CCGC 6 cut(s) 126, 132, 270, 960, 1064, 1098
AclWI GGATC 1 cut(s) 918
AcoI YGGCCR 1 cut(s) 280
AcsI RAATTY 1 cut(s) 707
AcuI CTGAAG 1 cut(s) 378
AfaI GTAC 2 cut(s) 626, 1108
AfiI CCNNNNNNNGG 2 cut(s) 119, 1111
AflIII ACRYGT 2 cut(s) 94, 989
AgsI TTSAA 2 cut(s) 265, 1157
AhdI GACNNNNNGTC 1 cut(s) 220
AjuI GAANNNNNNNTTGG 2 cut(s) 536, 568
AluBI AGCT 7 cut(s) 74, 362, 407, 676, 864, 1091, 1138
AluI AGCT 7 cut(s) 74, 362, 407, 676, 864, 1091, 1138
Alw21I GWGCWC 1 cut(s) 866
Alw26I GTCTC 2 cut(s) 177, 1017
AlwI GGATC 1 cut(s) 918
AlwNI CAGNNNCTG 1 cut(s) 410
AoxI GGCC 3 cut(s) 280, 756, 979
ApeKI GCWGC 6 cut(s) 404, 407, 527, 673, 957, 1135
ApoI RAATTY 1 cut(s) 707
Asp700I GAANNNNTTC 1 cut(s) 1091
AspS9I GGNCC 3 cut(s) 272, 757, 1102
AsuHPI GGTGA 4 cut(s) 67, 102, 380, 1078
AsuII TTCGAA 1 cut(s) 900
AvaII GGWCC 2 cut(s) 272, 1102
BaeGI GKGCMC 1 cut(s) 703
BanI GGYRCC 4 cut(s) 9, 87, 160, 698
BanII GRGCYC 1 cut(s) 866
BbsI GAAGAC 3 cut(s) 833, 909, 986
Bbv12I GWGCWC 1 cut(s) 866
BbvI GCAGC 6 cut(s) 394, 416, 514, 685, 944, 1147
BccI CCATC 1 cut(s) 52
BceAI ACGGC 2 cut(s) 267, 283
BcgI CGANNNNNNTGC 4 cut(s) 421, 455, 538, 572
BclI TGATCA 3 cut(s) 370, 493, 834
BcoDI GTCTC 2 cut(s) 177, 1017
BfaI CTAG 5 cut(s) 6, 329, 615, 681, 921
BfmI CTRYAG 1 cut(s) 402
BfuAI ACCTGC 1 cut(s) 785
BisI GCNGC 6 cut(s) 405, 408, 528, 674, 958, 1136
BlsI GCNGC 6 cut(s) 406, 409, 529, 675, 959, 1137
Bme18I GGWCC 2 cut(s) 272, 1102
BmeRI GACNNNNNGTC 1 cut(s) 220
BmgT120I GGNCC 3 cut(s) 272, 757, 1102
BmiI GGNNCC 7 cut(s) 11, 89, 162, 700, 775, 1103, 1163
BmsI GCATC 5 cut(s) 407, 514, 558, 944, 1033
BpiI GAAGAC 3 cut(s) 833, 909, 986
BpmI CTGGAG 1 cut(s) 96
Bpu14I TTCGAA 1 cut(s) 900
BpuEI CTTGAG 1 cut(s) 642
BsaI GGTCTC 1 cut(s) 1017
BsaJI CCNNGG 3 cut(s) 231, 303, 1110
Bsc4I CCNNNNNNNGG 2 cut(s) 119, 1111
Bse118I RCCGGY 1 cut(s) 119
Bse1I ACTGG 3 cut(s) 283, 433, 1165
Bse3DI GCAATG 1 cut(s) 580
BseDI CCNNGG 3 cut(s) 231, 303, 1110
BseGI GGATG 2 cut(s) 959, 1048
BseLI CCNNNNNNNGG 2 cut(s) 119, 1111
BseMI GCAATG 1 cut(s) 580
BseNI ACTGG 3 cut(s) 283, 433, 1165
BseRI GAGGAG 2 cut(s) 154, 902
BseSI GKGCMC 1 cut(s) 703
BseXI GCAGC 6 cut(s) 394, 416, 514, 685, 944, 1147
BshFI GGCC 3 cut(s) 282, 758, 981
BshNI GGYRCC 4 cut(s) 9, 87, 160, 698
BsiHKAI GWGCWC 1 cut(s) 866
BsiSI CCGG 1 cut(s) 120
BslFI GGGAC 1 cut(s) 1115
BslI CCNNNNNNNGG 2 cut(s) 119, 1111
BsmAI GTCTC 2 cut(s) 177, 1017
BsmFI GGGAC 1 cut(s) 1115
BsmI GAATGC 2 cut(s) 741, 1046
BsnI GGCC 3 cut(s) 282, 758, 981
Bso31I GGTCTC 1 cut(s) 1017
Bsp119I TTCGAA 1 cut(s) 900
Bsp1286I GDGCHC 2 cut(s) 703, 866
Bsp143I GATC 6 cut(s) 370, 493, 533, 646, 834, 923
Bsp19I CCATGG 1 cut(s) 303
BspACI CCGC 6 cut(s) 126, 132, 270, 960, 1064, 1098
BspANI GGCC 3 cut(s) 282, 758, 981
BspLI GGNNCC 7 cut(s) 11, 89, 162, 700, 775, 1103, 1163
BspMAI CTGCAG 1 cut(s) 406
BspMI ACCTGC 1 cut(s) 785
BspPI GGATC 1 cut(s) 918
BspT104I TTCGAA 1 cut(s) 900
BspT107I GGYRCC 4 cut(s) 9, 87, 160, 698
BspTNI GGTCTC 1 cut(s) 1017
BsrDI GCAATG 1 cut(s) 580
BsrFI RCCGGY 1 cut(s) 119
BsrI ACTGG 3 cut(s) 283, 433, 1165
BssAI RCCGGY 1 cut(s) 119
BssECI CCNNGG 3 cut(s) 231, 303, 1110
BssMI GATC 6 cut(s) 370, 493, 533, 646, 834, 923
BssT1I CCWWGG 2 cut(s) 231, 303
Bst4CI ACNGT 3 cut(s) 114, 160, 1106
BstBI TTCGAA 1 cut(s) 900
BstC8I GCNNGC 2 cut(s) 165, 1140
BstDSI CCRYGG 1 cut(s) 303
BstF5I GGATG 2 cut(s) 959, 1048
BstKTI GATC 6 cut(s) 373, 496, 536, 649, 837, 926
BstMAI GTCTC 2 cut(s) 177, 1017
BstMBI GATC 6 cut(s) 370, 493, 533, 646, 834, 923
BstMWI GCNNNNNNNGC 2 cut(s) 404, 524
BstNSI RCATGY 3 cut(s) 98, 910, 993
BstSFI CTRYAG 1 cut(s) 402
BstSLI GKGCMC 1 cut(s) 703
BstV1I GCAGC 6 cut(s) 394, 416, 514, 685, 944, 1147
BstV2I GAAGAC 3 cut(s) 833, 909, 986
BstX2I RGATCY 1 cut(s) 923
BstYI RGATCY 1 cut(s) 923
BsuRI GGCC 3 cut(s) 282, 758, 981
BtgI CCRYGG 1 cut(s) 303
BtgZI GCGATG 1 cut(s) 871
BtsCI GGATG 2 cut(s) 959, 1048
BtsIMutI CAGTG 1 cut(s) 426
BveI ACCTGC 1 cut(s) 785
Cac8I GCNNGC 2 cut(s) 165, 1140
CaiI CAGNNNCTG 1 cut(s) 410
Cfr10I RCCGGY 1 cut(s) 119
Cfr13I GGNCC 3 cut(s) 272, 757, 1102
CseI GACGC 1 cut(s) 368
Csp6I GTAC 2 cut(s) 625, 1107
CviAII CATG 8 cut(s) 95, 169, 304, 383, 907, 990, 1011, 1123
CviQI GTAC 2 cut(s) 625, 1107
DpnI GATC 6 cut(s) 372, 495, 535, 648, 836, 925
DpnII GATC 6 cut(s) 370, 493, 533, 646, 834, 923
DriI GACNNNNNGTC 1 cut(s) 220
EaeI YGGCCR 1 cut(s) 280
Eam1105I GACNNNNNGTC 1 cut(s) 220
Ecl136II GAGCTC 1 cut(s) 864
Eco130I CCWWGG 2 cut(s) 231, 303
Eco24I GRGCYC 1 cut(s) 866
Eco31I GGTCTC 1 cut(s) 1017
Eco47I GGWCC 2 cut(s) 272, 1102
Eco53kI GAGCTC 1 cut(s) 864
Eco57I CTGAAG 1 cut(s) 378
EcoICRI GAGCTC 1 cut(s) 864
EcoT14I CCWWGG 2 cut(s) 231, 303
EcoT22I ATGCAT 2 cut(s) 400, 1048
EcoT38I GRGCYC 1 cut(s) 866
ErhI CCWWGG 2 cut(s) 231, 303
FaeI CATG 8 cut(s) 98, 172, 307, 386, 910, 993, 1014, 1126
FalI AAGNNNNNCTT 4 cut(s) 439, 471, 536, 568
FaqI GGGAC 1 cut(s) 1115
FatI CATG 8 cut(s) 94, 168, 303, 382, 906, 989, 1010, 1122
FauI CCCGC 1 cut(s) 1091
FbaI TGATCA 3 cut(s) 370, 493, 834
Fnu4HI GCNGC 6 cut(s) 405, 408, 528, 674, 958, 1136
FokI GGATG 2 cut(s) 966, 1055
FriOI GRGCYC 1 cut(s) 866
Fsp4HI GCNGC 6 cut(s) 405, 408, 528, 674, 958, 1136
FspBI CTAG 5 cut(s) 6, 329, 615, 681, 921
GluI GCNGC 6 cut(s) 405, 408, 528, 674, 958, 1136
GsuI CTGGAG 1 cut(s) 96
HaeIII GGCC 3 cut(s) 282, 758, 981
HapII CCGG 1 cut(s) 120
HgaI GACGC 1 cut(s) 368
Hin1II CATG 8 cut(s) 98, 172, 307, 386, 910, 993, 1014, 1126
HindIII AAGCTT 2 cut(s) 360, 1089
HinfI GANTC 6 cut(s) 185, 343, 387, 414, 619, 933
HpaII CCGG 1 cut(s) 120
HphI GGTGA 4 cut(s) 67, 102, 380, 1078
Hpy166II GTNNAC 3 cut(s) 100, 464, 1069
Hpy188I TCNGA 6 cut(s) 154, 322, 358, 483, 498, 784
Hpy188III TCNNGA 5 cut(s) 68, 659, 921, 1022, 1148
Hpy8I GTNNAC 3 cut(s) 100, 464, 1069
HpyAV CCTTC 1 cut(s) 23
HpyCH4III ACNGT 3 cut(s) 114, 160, 1106
HpyCH4V TGCA 6 cut(s) 398, 404, 549, 741, 944, 1046
HpyF10VI GCNNNNNNNGC 2 cut(s) 404, 524
Hsp92II CATG 8 cut(s) 98, 172, 307, 386, 910, 993, 1014, 1126
Ksp22I TGATCA 3 cut(s) 370, 493, 834
Kzo9I GATC 6 cut(s) 370, 493, 533, 646, 834, 923
LmnI GCTCC 1 cut(s) 915
Lsp1109I GCAGC 6 cut(s) 394, 416, 514, 685, 944, 1147
LweI GCATC 5 cut(s) 407, 514, 558, 944, 1033
MaeI CTAG 5 cut(s) 6, 329, 615, 681, 921
MaeIII GTNAC 2 cut(s) 971, 985
MalI GATC 6 cut(s) 372, 495, 535, 648, 836, 925
MboI GATC 6 cut(s) 370, 493, 533, 646, 834, 923
MboII GAAGA 8 cut(s) 459, 544, 547, 741, 838, 914, 986, 1027
MflI RGATCY 1 cut(s) 923
MhlI GDGCHC 2 cut(s) 703, 866
MluCI AATT 6 cut(s) 192, 391, 589, 707, 916, 1077
MlyI GAGTC 2 cut(s) 179, 396
MmeI TCCRAC 2 cut(s) 541, 635
MnlI CCTC 5 cut(s) 132, 923, 971, 1077, 1105
Mph1103I ATGCAT 2 cut(s) 400, 1048
MroXI GAANNNNTTC 1 cut(s) 1091
MseI TTAA 3 cut(s) 792, 813, 846
MspA1I CMGCKG 3 cut(s) 407, 1064, 1098
MspI CCGG 1 cut(s) 120
Mva1269I GAATGC 2 cut(s) 741, 1046
MwoI GCNNNNNNNGC 2 cut(s) 404, 524
NcoI CCATGG 1 cut(s) 303
NdeII GATC 6 cut(s) 370, 493, 533, 646, 834, 923
NlaIII CATG 8 cut(s) 98, 172, 307, 386, 910, 993, 1014, 1126
NlaIV GGNNCC 7 cut(s) 11, 89, 162, 700, 775, 1103, 1163
NsiI ATGCAT 2 cut(s) 400, 1048
NspI RCATGY 3 cut(s) 98, 910, 993
NspV TTCGAA 1 cut(s) 900
PciI ACATGT 2 cut(s) 94, 989
PctI GAATGC 2 cut(s) 741, 1046
PdmI GAANNNNTTC 1 cut(s) 1091
PfeI GAWTC 4 cut(s) 343, 414, 619, 933
PkrI GCNGC 6 cut(s) 406, 409, 529, 675, 959, 1137
PleI GAGTC 2 cut(s) 179, 395
PpsI GAGTC 2 cut(s) 179, 395
PscI ACATGT 2 cut(s) 94, 989
Psp124BI GAGCTC 1 cut(s) 866
PspN4I GGNNCC 7 cut(s) 11, 89, 162, 700, 775, 1103, 1163
PspPI GGNCC 3 cut(s) 272, 757, 1102
PstI CTGCAG 1 cut(s) 406
PstNI CAGNNNCTG 1 cut(s) 410
PsuI RGATCY 1 cut(s) 923
PvuII CAGCTG 1 cut(s) 407
RsaI GTAC 2 cut(s) 626, 1108
RsaNI GTAC 2 cut(s) 625, 1107
SacI GAGCTC 1 cut(s) 866
SaqAI TTAA 3 cut(s) 792, 813, 846
SatI GCNGC 6 cut(s) 405, 408, 528, 674, 958, 1136
Sau3AI GATC 6 cut(s) 370, 493, 533, 646, 834, 923
Sau96I GGNCC 3 cut(s) 272, 757, 1102
SchI GAGTC 2 cut(s) 179, 396
SduI GDGCHC 2 cut(s) 703, 866
SfaNI GCATC 5 cut(s) 407, 514, 558, 944, 1033
SfcI CTRYAG 1 cut(s) 402
SfuI TTCGAA 1 cut(s) 900
SinI GGWCC 2 cut(s) 272, 1102
SmlI CTYRAG 1 cut(s) 657
SmoI CTYRAG 1 cut(s) 657
Sse9I AATT 6 cut(s) 192, 391, 589, 707, 916, 1077
SsiI CCGC 6 cut(s) 126, 132, 270, 960, 1064, 1098
SspMI CTAG 5 cut(s) 6, 329, 615, 681, 921
SstI GAGCTC 1 cut(s) 866
StyI CCWWGG 2 cut(s) 231, 303
TaaI ACNGT 3 cut(s) 114, 160, 1106
TaqI TCGA 3 cut(s) 532, 900, 1021
TasI AATT 6 cut(s) 192, 391, 589, 707, 916, 1077
TatI WGTACW 1 cut(s) 624
TfiI GAWTC 4 cut(s) 343, 414, 619, 933
Tru1I TTAA 3 cut(s) 792, 813, 846
Tru9I TTAA 3 cut(s) 792, 813, 846
TscAI CASTG 1 cut(s) 433
TseI GCWGC 6 cut(s) 404, 407, 527, 673, 957, 1135
TspDTI ATGAA 3 cut(s) 327, 862, 1139
TspGWI ACGGA 2 cut(s) 210, 801
TspRI CASTG 1 cut(s) 433
VpaK11BI GGWCC 2 cut(s) 272, 1102
XapI RAATTY 1 cut(s) 707
XbaI TCTAGA 1 cut(s) 920
XceI RCATGY 3 cut(s) 98, 910, 993
XcmI CCANNNNNNNNNTGG 1 cut(s) 767
XmnI GAANNNNTTC 1 cut(s) 1091
XspI CTAG 5 cut(s) 6, 329, 615, 681, 921
Zsp2I ATGCAT 2 cut(s) 400, 1048
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.