FvH4_5g36252

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0013874
Physical Location & Seq
Reverse (-)
26600360 .. 26601409
1050 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g36252.t1

Sequence Viewer

Length: 1050 bp
ATGGCGGTGATGAAACACAGGAAACTCTTCCCAGCACCATTTTCCTCAAACCAAACTGACTGTGCAGACTGCCCAGATTGCACGTATGATTGTTACCCTTATACAGATTTCTATATTCCCCCACCACCACCATTTCCAGCCATTGAACAAAGTGATCAGAACAGCCATATCTCACCATATGTGATTCTGTTCCTCTGTTTGCTAGCTGGTCTTTTCCTTCTCATTGCCTACTATGTCATTATAGTGAAGTCCTGTGCAAGTTTCTGCAGTCCTAATAGGAACAATGGATCTCCAGAGTCCGAGTCAAACAGCGGAGATGGAGAATTTTTTGCAGAGAATCAAGTTGATCACCCAATATGGTTCATCACCACCGCTGGCTTGCAGCAATCCATCATAAACTCGATCACGGTATGTAAGTACAAGAAATGTGATGGCTTGATTGAAGGGACAGAGTGCTCTGTTTGTTTAAATGAGTTTCATGAAGATGAGATGCTTAGATTATTGCCTAAATGCAGTCATGCTTTTCATATAAATTGTATTGATACTTGGTTGAGCTCTCACACCAATTGCCCCCTATGCCGTGCCTATATTACTTCTGAGACTCTCGATAATTCACACTTTGCGAGGCTTGATTTGGGTTCAGGGGATCGAAATGGGGATGGTGTAGAAACAAATGGAGGTTCCCTCATGGAGATTTCTGAGATTGAAATTGAAGTAGATGTTAATAATCAAGTGAGGAATGATAATCAGGTTTGTGAAAACAGAGCTGGAACAGAGGATGAAGGTGAAGAATACCAGGTTGGTAATAGAACTACATCAAAGGAGGTAATAACTTTGAATGAAGTTGCATTAAGAAGGTCTATTTCGATGGATTCTTCATTGGCTGAAACCAAGTTACATTCAGATATTGCTCGAAATCGTCAGTCTTCAATCGAGCAGTGTCTTCATATAAGTCCTGTTTTGATGAAAAGGTCTTTTTCTTGTGGTGGAAGGTCGAGATTGTTGTCTTTGAGACATAAATGGGGGATGAATACAGTTGTGCCCCTCTGA

Protein Analysis

350

Amino Acids

39.0

Weight (kDa)

5.03

Isoelectric Point (pI)

62.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 151 - 194 3.1e-13 Ring finger domain
zf-RING_11 PF17123 151 - 180 4.3e-10 RING-like zinc finger
zf-C3HC4_2 PF13923 151 - 193 3.4e-07 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 152 - 194 1.1e-08 RING-H2 zinc finger domain
zf-C3HC4 PF00097 152 - 193 7.3e-07 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013874)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G72220
fragaria_vesca FvH4_5g36252
malus_domestica MD08G1190400.v1.1 MD15G1377700.v1.1
prunus_persica Prupe.1G524400_v2.0.a1
pyrus_communis pycom08g16360 pycom15g33840
rosa_chinensis RchiOBHm_Chr7g0238151
rosa_laevigata RLG00000000938
rosa_multiflora Rmu_sc0008459.1_g000001
rosa_roxburghii Rroxscaffold_3G00223320
rosa_rugosa Rorug07G0310600
rosa_samantha Rh7AG466300 Rh7BG436800 Rh7CG484100 Rh7DG452500
rosa_wichuraiana Rw7G038610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 5, 312, 372
AclWI GGATC 2 cut(s) 295, 654
AcsI RAATTY 1 cut(s) 323
AfaI GTAC 1 cut(s) 419
AgsI TTSAA 6 cut(s) 146, 443, 707, 713, 838, 930
AjnI CCWGG 1 cut(s) 795
AjuI GAANNNNNNNTTGG 2 cut(s) 783, 815
AluBI AGCT 3 cut(s) 206, 555, 767
AluI AGCT 3 cut(s) 206, 555, 767
Alw21I GWGCWC 2 cut(s) 458, 557
Alw26I GTCTC 2 cut(s) 593, 1006
AlwI GGATC 2 cut(s) 295, 654
ApeKI GCWGC 1 cut(s) 382
ApoI RAATTY 1 cut(s) 323
Asp700I GAANNNNTTC 1 cut(s) 26
AsuHPI GGTGA 5 cut(s) 19, 165, 341, 358, 797
AsuNHI GCTAGC 1 cut(s) 202
BaeGI GKGCMC 1 cut(s) 1044
BanII GRGCYC 1 cut(s) 557
BbsI GAAGAC 2 cut(s) 918, 935
Bbv12I GWGCWC 2 cut(s) 458, 557
BbvI GCAGC 1 cut(s) 394
BccI CCATC 5 cut(s) 311, 398, 425, 653, 862
BceAI ACGGC 1 cut(s) 564
BciT130I CCWGG 1 cut(s) 797
BclI TGATCA 2 cut(s) 154, 346
BcoDI GTCTC 2 cut(s) 593, 1006
BfaI CTAG 1 cut(s) 203
BfmI CTRYAG 1 cut(s) 265
BisI GCNGC 1 cut(s) 383
BlsI GCNGC 1 cut(s) 384
Bme1390I CCNGG 1 cut(s) 797
BmiI GGNNCC 1 cut(s) 682
BmrFI CCNGG 1 cut(s) 797
BmsI GCATC 1 cut(s) 480
BmtI GCTAGC 1 cut(s) 206
BpiI GAAGAC 2 cut(s) 918, 935
BplI GAGNNNNNCTC 2 cut(s) 669, 701
BpmI CTGGAG 1 cut(s) 276
BsaAI YACGTR 1 cut(s) 84
Bse3DI GCAATG 1 cut(s) 222
BseBI CCWGG 1 cut(s) 797
BseGI GGATG 3 cut(s) 664, 784, 1032
BseMI GCAATG 1 cut(s) 222
BseMII CTCAG 2 cut(s) 588, 690
BseSI GKGCMC 1 cut(s) 1044
BseXI GCAGC 1 cut(s) 394
BseYI CCCAGC 1 cut(s) 31
BsgI GTGCAG 1 cut(s) 84
BsiHKAI GWGCWC 2 cut(s) 458, 557
BslFI GGGAC 1 cut(s) 460
BsmAI GTCTC 2 cut(s) 593, 1006
BsmFI GGGAC 1 cut(s) 460
Bsp1286I GDGCHC 3 cut(s) 458, 557, 1044
Bsp143I GATC 5 cut(s) 154, 287, 346, 402, 646
BspACI CCGC 3 cut(s) 5, 312, 372
BspCNI CTCAG 2 cut(s) 589, 691
BspHI TCATGA 1 cut(s) 478
BspLI GGNNCC 1 cut(s) 682
BspMAI CTGCAG 1 cut(s) 269
BspOI GCTAGC 1 cut(s) 206
BspPI GGATC 2 cut(s) 295, 654
BsrDI GCAATG 1 cut(s) 222
BssMI GATC 5 cut(s) 154, 287, 346, 402, 646
Bst2UI CCWGG 1 cut(s) 797
Bst4CI ACNGT 3 cut(s) 62, 409, 1036
Bst6I CTCTTC 1 cut(s) 32
BstBAI YACGTR 1 cut(s) 84
BstC8I GCNNGC 3 cut(s) 204, 376, 380
BstDEI CTNAG 3 cut(s) 494, 597, 699
BstF5I GGATG 3 cut(s) 664, 784, 1032
BstKTI GATC 5 cut(s) 157, 290, 349, 405, 649
BstMAI GTCTC 2 cut(s) 593, 1006
BstMBI GATC 5 cut(s) 154, 287, 346, 402, 646
BstMWI GCNNNNNNNGC 2 cut(s) 78, 576
BstNI CCWGG 1 cut(s) 797
BstSCI CCNGG 1 cut(s) 795
BstSFI CTRYAG 1 cut(s) 265
BstSLI GKGCMC 1 cut(s) 1044
BstV1I GCAGC 1 cut(s) 394
BstV2I GAAGAC 2 cut(s) 918, 935
BstX2I RGATCY 1 cut(s) 287
BstYI RGATCY 1 cut(s) 287
BtsCI GGATG 3 cut(s) 664, 784, 1032
BtsI GCAGTG 1 cut(s) 944
BtsIMutI CAGTG 1 cut(s) 944
Cac8I GCNNGC 3 cut(s) 204, 376, 380
CciI TCATGA 1 cut(s) 478
CsiI ACCWGGT 1 cut(s) 795
Csp6I GTAC 1 cut(s) 418
CviAII CATG 3 cut(s) 479, 518, 688
CviJI RGCY 9 cut(s) 140, 165, 206, 378, 435, 555, 628, 767, 884
CviKI_1 RGCY 9 cut(s) 140, 165, 206, 378, 435, 555, 628, 767, 884
CviQI GTAC 1 cut(s) 418
DdeI CTNAG 3 cut(s) 494, 597, 699
DpnI GATC 5 cut(s) 156, 289, 348, 404, 648
DpnII GATC 5 cut(s) 154, 287, 346, 402, 646
DraI TTTAAA 1 cut(s) 468
Eam1104I CTCTTC 1 cut(s) 32
EarI CTCTTC 1 cut(s) 32
Ecl136II GAGCTC 1 cut(s) 555
Eco24I GRGCYC 1 cut(s) 557
Eco53kI GAGCTC 1 cut(s) 555
EcoICRI GAGCTC 1 cut(s) 555
EcoRII CCWGG 1 cut(s) 795
EcoT38I GRGCYC 1 cut(s) 557
FaeI CATG 3 cut(s) 482, 521, 691
FaqI GGGAC 1 cut(s) 460
FatI CATG 3 cut(s) 478, 517, 687
FauNDI CATATG 1 cut(s) 178
FbaI TGATCA 2 cut(s) 154, 346
Fnu4HI GCNGC 1 cut(s) 383
FokI GGATG 3 cut(s) 671, 791, 1039
FriOI GRGCYC 1 cut(s) 557
Fsp4HI GCNGC 1 cut(s) 383
FspBI CTAG 1 cut(s) 203
GluI GCNGC 1 cut(s) 383
GsaI CCCAGC 1 cut(s) 35
GsuI CTGGAG 1 cut(s) 276
Hin1II CATG 3 cut(s) 482, 521, 691
HinfI GANTC 6 cut(s) 184, 296, 302, 337, 601, 872
HphI GGTGA 5 cut(s) 19, 165, 341, 358, 797
Hpy188I TCNGA 6 cut(s) 159, 301, 598, 700, 904, 1049
Hpy188III TCNNGA 4 cut(s) 293, 479, 605, 996
HpyAV CCTTC 5 cut(s) 227, 437, 776, 849, 984
HpyCH4III ACNGT 3 cut(s) 62, 409, 1036
HpyCH4IV ACGT 1 cut(s) 83
HpyCH4V TGCA 8 cut(s) 65, 81, 257, 267, 332, 382, 513, 848
HpyF10VI GCNNNNNNNGC 2 cut(s) 78, 576
HpyF3I CTNAG 3 cut(s) 494, 597, 699
HpySE526I ACGT 1 cut(s) 83
Hsp92II CATG 3 cut(s) 482, 521, 691
Ksp22I TGATCA 2 cut(s) 154, 346
Kzo9I GATC 5 cut(s) 154, 287, 346, 402, 646
Lsp1109I GCAGC 1 cut(s) 394
LweI GCATC 1 cut(s) 480
MabI ACCWGGT 1 cut(s) 795
MaeI CTAG 1 cut(s) 203
MaeII ACGT 1 cut(s) 83
MaeIII GTNAC 2 cut(s) 92, 894
MalI GATC 5 cut(s) 156, 289, 348, 404, 648
MboI GATC 5 cut(s) 154, 287, 346, 402, 646
MboII GAAGA 6 cut(s) 19, 494, 800, 867, 918, 935
MfeI CAATTG 1 cut(s) 565
MflI RGATCY 1 cut(s) 287
MhlI GDGCHC 3 cut(s) 458, 557, 1044
MluCI AATT 5 cut(s) 323, 532, 565, 610, 708
MlyI GAGTC 3 cut(s) 305, 311, 595
MnlI CCTC 8 cut(s) 55, 203, 618, 671, 695, 729, 769, 817
MroXI GAANNNNTTC 1 cut(s) 26
MseI TTAA 3 cut(s) 467, 723, 851
MslI CAYNNNNRTG 2 cut(s) 242, 483
MspA1I CMGCKG 2 cut(s) 312, 374
MspR9I CCNGG 1 cut(s) 797
MunI CAATTG 1 cut(s) 565
MvaI CCWGG 1 cut(s) 797
MwoI GCNNNNNNNGC 2 cut(s) 78, 576
NdeI CATATG 1 cut(s) 178
NdeII GATC 5 cut(s) 154, 287, 346, 402, 646
NheI GCTAGC 1 cut(s) 202
NlaIII CATG 3 cut(s) 482, 521, 691
NlaIV GGNNCC 1 cut(s) 682
PagI TCATGA 1 cut(s) 478
PdmI GAANNNNTTC 1 cut(s) 26
PfeI GAWTC 3 cut(s) 184, 337, 872
PkrI GCNGC 1 cut(s) 384
PleI GAGTC 3 cut(s) 304, 310, 595
PpsI GAGTC 3 cut(s) 304, 310, 595
Ppu21I YACGTR 1 cut(s) 84
Psp124BI GAGCTC 1 cut(s) 557
Psp6I CCWGG 1 cut(s) 795
PspFI CCCAGC 1 cut(s) 31
PspGI CCWGG 1 cut(s) 795
PspN4I GGNNCC 1 cut(s) 682
PstI CTGCAG 1 cut(s) 269
PsuI RGATCY 1 cut(s) 287
RsaI GTAC 1 cut(s) 419
RsaNI GTAC 1 cut(s) 418
RseI CAYNNNNRTG 2 cut(s) 242, 483
SacI GAGCTC 1 cut(s) 557
SaqAI TTAA 3 cut(s) 467, 723, 851
SatI GCNGC 1 cut(s) 383
Sau3AI GATC 5 cut(s) 154, 287, 346, 402, 646
SchI GAGTC 3 cut(s) 305, 311, 595
ScrFI CCNGG 1 cut(s) 797
SduI GDGCHC 3 cut(s) 458, 557, 1044
SexAI ACCWGGT 1 cut(s) 795
SfaNI GCATC 1 cut(s) 480
SfcI CTRYAG 1 cut(s) 265
SmiMI CAYNNNNRTG 2 cut(s) 242, 483
Sse9I AATT 5 cut(s) 323, 532, 565, 610, 708
SsiI CCGC 3 cut(s) 5, 312, 372
SspMI CTAG 1 cut(s) 203
SstI GAGCTC 1 cut(s) 557
StyD4I CCNGG 1 cut(s) 795
TaaI ACNGT 3 cut(s) 62, 409, 1036
TaiI ACGT 1 cut(s) 86
TaqI TCGA 7 cut(s) 401, 606, 649, 866, 913, 933, 995
TasI AATT 5 cut(s) 323, 532, 565, 610, 708
TatI WGTACW 1 cut(s) 417
TfiI GAWTC 3 cut(s) 184, 337, 872
Tru1I TTAA 3 cut(s) 467, 723, 851
Tru9I TTAA 3 cut(s) 467, 723, 851
TscAI CASTG 1 cut(s) 944
TseI GCWGC 1 cut(s) 382
TspRI CASTG 1 cut(s) 944
XapI RAATTY 1 cut(s) 323
XmnI GAANNNNTTC 1 cut(s) 26
XspI CTAG 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.