Rh7DG452500

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0013874
Physical Location & Seq
Reverse (-)
64457522 .. 64459362
1841 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG452500.1

Sequence Viewer

Length: 1050 bp
ATGGCGGTGATCAGACACAGGAAACTCTTCCCAGCACCATTTTCCTCAAACCAAACTGACTGCCCAGACTGCCCAGATTGCACATATGATTGTTACCCCTATACAGATTTCTATATTCCCCCACCACCACCATTTCCTGCCATTGATCAAAATGACCAAAACCACCATATCTCACCTTATGTGATTCTGTTCCTCTCTTTGCTAGCTGGGCTTTTCCTTCTCATTGCCTACTATGTCATTATAGTAAAGTCCTGTACAAGTTTCTGCAGTCCTAATAGAAACAATGGATCTCCACAGTCCGAATCAACCAGCGGAGATGAGGAATTTCTTGCAGAGAATCAAGTTGATCACCCAATATGGTTCATCACCACCGTTGGCTTGCAGCAATCTATCATAAATTCTATCACAGTTTGTAAGTACAAGAAATGTGAGGGCTTGATTGAAGGGACAGAGTGCTCTGTTTGTTTAAATGAGTTCCATGAAGATGAGATGCTTAGATTATTGCCCAAGTGTAGTCATGCTTTTCATATGAATTGTATTGATACTTGGTTGAGGTCTCACACAAATTGCCCTCTATGCCGTGCTTATATTACTTCTGAGACTCTCGGTAATTCAAACATTGTGAGGCTTGATTTGGGTTCCGGAGATCAAAATGGGGATGGTATAGACACAAATGGAGATACCCTGATGGAGATTTCTGAGATTGAAAGTGAATTGGATGTGAATATTCAAGTGGGATATGACAATCAGGTGTGTGAAAACAGAGCTGGAACAGAGGATGAAGGTGAAGAACACCAGTTTGGCAATGGAACTACATCAAAAGAGGTAATAACTCTTAATGAAGTTCCATTAAGAAGGTCTATTTCAATGGATTCTTCGTCGGCTGAAACCAAGTTCCATTCAGATGTTGCTCGAAATCGTCAGTCTTCAATTCAGCAATGTCTTCATATAAGTCCTGTTTTGATGAAAAGGTCTTTTTCTTGTGGTGGAAGGTCGAGATTGTTGTCTTTGAGACATAAATGGGGGATGAATTCAGTTGTTCCCCTCTGA

Protein Analysis

349

Amino Acids

39.09

Weight (kDa)

5.0

Isoelectric Point (pI)

62.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 151 - 194 6e-14 Ring finger domain
zf-RING_11 PF17123 151 - 180 2.4e-10 RING-like zinc finger
zf-C3HC4_2 PF13923 151 - 193 9.6e-08 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 152 - 194 2.6e-09 RING-H2 zinc finger domain
zf-C3HC4 PF00097 152 - 193 1.1e-06 Zinc finger, C3HC4 type (RING finger)
zf-ANAPC11 PF12861 158 - 197 8.3e-06 Anaphase-promoting complex subunit 11 RING-H2 finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013874)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G72220
fragaria_vesca FvH4_5g36252
malus_domestica MD08G1190400.v1.1 MD15G1377700.v1.1
prunus_persica Prupe.1G524400_v2.0.a1
pyrus_communis pycom08g16360 pycom15g33840
rosa_chinensis RchiOBHm_Chr7g0238151
rosa_laevigata RLG00000000938
rosa_multiflora Rmu_sc0008459.1_g000001
rosa_roxburghii Rroxscaffold_3G00223320
rosa_rugosa Rorug07G0310600
rosa_samantha Rh7AG466300 Rh7BG436800 Rh7CG484100 Rh7DG452500
rosa_wichuraiana Rw7G038610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 641
AciI CCGC 2 cut(s) 5, 312
AclWI GGATC 1 cut(s) 295
AcsI RAATTY 3 cut(s) 323, 397, 1030
AfaI GTAC 2 cut(s) 256, 419
AgsI TTSAA 6 cut(s) 443, 615, 707, 731, 867, 930
AjuI GAANNNNNNNTTGG 2 cut(s) 783, 815
AluBI AGCT 2 cut(s) 206, 767
AluI AGCT 2 cut(s) 206, 767
Alw21I GWGCWC 1 cut(s) 458
Alw26I GTCTC 3 cut(s) 561, 593, 1006
AlwI GGATC 1 cut(s) 295
Aor13HI TCCGGA 1 cut(s) 641
ApeKI GCWGC 1 cut(s) 382
ApoI RAATTY 3 cut(s) 323, 397, 1030
Asp700I GAANNNNTTC 1 cut(s) 26
AsuHPI GGTGA 5 cut(s) 19, 165, 341, 358, 797
AsuNHI GCTAGC 1 cut(s) 202
BbsI GAAGAC 2 cut(s) 918, 935
Bbv12I GWGCWC 1 cut(s) 458
BbvI GCAGC 1 cut(s) 394
BccI CCATC 2 cut(s) 653, 682
BceAI ACGGC 1 cut(s) 564
BclI TGATCA 3 cut(s) 9, 145, 346
BcoDI GTCTC 3 cut(s) 561, 593, 1006
BfaI CTAG 1 cut(s) 203
BfmI CTRYAG 1 cut(s) 265
BisI GCNGC 1 cut(s) 383
BlsI GCNGC 1 cut(s) 384
BmiI GGNNCC 1 cut(s) 640
BmsI GCATC 1 cut(s) 480
BmtI GCTAGC 1 cut(s) 206
BpiI GAAGAC 2 cut(s) 918, 935
BsaI GGTCTC 1 cut(s) 561
BsaWI WCCGGW 1 cut(s) 641
Bse1I ACTGG 1 cut(s) 796
Bse3DI GCAATG 3 cut(s) 222, 811, 944
BseAI TCCGGA 1 cut(s) 641
BseGI GGATG 4 cut(s) 664, 724, 784, 1032
BseMI GCAATG 3 cut(s) 222, 811, 944
BseMII CTCAG 2 cut(s) 588, 690
BseNI ACTGG 1 cut(s) 796
BseXI GCAGC 1 cut(s) 394
BseYI CCCAGC 2 cut(s) 31, 206
BsiHKAI GWGCWC 1 cut(s) 458
BsiSI CCGG 1 cut(s) 642
BslFI GGGAC 1 cut(s) 460
BsmAI GTCTC 3 cut(s) 561, 593, 1006
BsmFI GGGAC 1 cut(s) 460
Bso31I GGTCTC 1 cut(s) 561
Bsp1286I GDGCHC 1 cut(s) 458
Bsp13I TCCGGA 1 cut(s) 641
Bsp1407I TGTACA 1 cut(s) 254
Bsp143I GATC 5 cut(s) 9, 145, 287, 346, 646
BspACI CCGC 2 cut(s) 5, 312
BspCNI CTCAG 2 cut(s) 589, 691
BspEI TCCGGA 1 cut(s) 641
BspLI GGNNCC 1 cut(s) 640
BspMAI CTGCAG 1 cut(s) 269
BspOI GCTAGC 1 cut(s) 206
BspPI GGATC 1 cut(s) 295
BspTNI GGTCTC 1 cut(s) 561
BsrDI GCAATG 3 cut(s) 222, 811, 944
BsrGI TGTACA 1 cut(s) 254
BsrI ACTGG 1 cut(s) 796
BssMI GATC 5 cut(s) 9, 145, 287, 346, 646
Bst4CI ACNGT 3 cut(s) 297, 373, 409
Bst6I CTCTTC 1 cut(s) 32
BstAUI TGTACA 1 cut(s) 254
BstC8I GCNNGC 2 cut(s) 204, 380
BstDEI CTNAG 3 cut(s) 494, 597, 699
BstF5I GGATG 4 cut(s) 664, 724, 784, 1032
BstKTI GATC 5 cut(s) 12, 148, 290, 349, 649
BstMAI GTCTC 3 cut(s) 561, 593, 1006
BstMBI GATC 5 cut(s) 9, 145, 287, 346, 646
BstMWI GCNNNNNNNGC 4 cut(s) 69, 78, 208, 576
BstSFI CTRYAG 1 cut(s) 265
BstV1I GCAGC 1 cut(s) 394
BstV2I GAAGAC 2 cut(s) 918, 935
BstX2I RGATCY 1 cut(s) 287
BstYI RGATCY 1 cut(s) 287
BtsCI GGATG 4 cut(s) 664, 724, 784, 1032
Cac8I GCNNGC 2 cut(s) 204, 380
Csp6I GTAC 2 cut(s) 255, 418
CviAII CATG 2 cut(s) 479, 518
CviJI RGCY 7 cut(s) 206, 211, 378, 435, 628, 767, 884
CviKI_1 RGCY 7 cut(s) 206, 211, 378, 435, 628, 767, 884
CviQI GTAC 2 cut(s) 255, 418
DdeI CTNAG 3 cut(s) 494, 597, 699
DpnI GATC 5 cut(s) 11, 147, 289, 348, 648
DpnII GATC 5 cut(s) 9, 145, 287, 346, 646
DraI TTTAAA 1 cut(s) 468
Eam1104I CTCTTC 1 cut(s) 32
EarI CTCTTC 1 cut(s) 32
Eco31I GGTCTC 1 cut(s) 561
EcoRI GAATTC 1 cut(s) 1030
FaeI CATG 2 cut(s) 482, 521
FaqI GGGAC 1 cut(s) 460
FatI CATG 2 cut(s) 478, 517
FauNDI CATATG 2 cut(s) 85, 528
FbaI TGATCA 3 cut(s) 9, 145, 346
Fnu4HI GCNGC 1 cut(s) 383
FokI GGATG 4 cut(s) 671, 731, 791, 1039
Fsp4HI GCNGC 1 cut(s) 383
FspBI CTAG 1 cut(s) 203
GluI GCNGC 1 cut(s) 383
GsaI CCCAGC 2 cut(s) 35, 210
HapII CCGG 1 cut(s) 642
Hin1II CATG 2 cut(s) 482, 521
HinfI GANTC 5 cut(s) 184, 302, 337, 601, 872
HpaII CCGG 1 cut(s) 642
HphI GGTGA 5 cut(s) 19, 165, 341, 358, 797
Hpy188I TCNGA 6 cut(s) 14, 301, 598, 700, 904, 1049
Hpy188III TCNNGA 2 cut(s) 642, 996
Hpy99I CGWCG 1 cut(s) 883
HpyAV CCTTC 5 cut(s) 227, 437, 776, 849, 984
HpyCH4III ACNGT 3 cut(s) 297, 373, 409
HpyCH4V TGCA 4 cut(s) 81, 267, 332, 382
HpyF10VI GCNNNNNNNGC 4 cut(s) 69, 78, 208, 576
HpyF3I CTNAG 3 cut(s) 494, 597, 699
Hsp92II CATG 2 cut(s) 482, 521
Kpn2I TCCGGA 1 cut(s) 641
Ksp22I TGATCA 3 cut(s) 9, 145, 346
Kzo9I GATC 5 cut(s) 9, 145, 287, 346, 646
Lsp1109I GCAGC 1 cut(s) 394
LweI GCATC 1 cut(s) 480
MaeI CTAG 1 cut(s) 203
MaeIII GTNAC 1 cut(s) 92
MalI GATC 5 cut(s) 11, 147, 289, 348, 648
MboI GATC 5 cut(s) 9, 145, 287, 346, 646
MboII GAAGA 6 cut(s) 19, 494, 800, 867, 918, 935
MflI RGATCY 1 cut(s) 287
MhlI GDGCHC 1 cut(s) 458
MluCI AATT 8 cut(s) 323, 397, 532, 565, 610, 713, 930, 1030
MlyI GAGTC 1 cut(s) 595
MnlI CCTC 9 cut(s) 55, 203, 313, 424, 546, 582, 618, 769, 817
MroI TCCGGA 1 cut(s) 641
MroXI GAANNNNTTC 1 cut(s) 26
MseI TTAA 3 cut(s) 467, 837, 851
MslI CAYNNNNRTG 2 cut(s) 483, 903
MspA1I CMGCKG 1 cut(s) 312
MspI CCGG 1 cut(s) 642
MwoI GCNNNNNNNGC 4 cut(s) 69, 78, 208, 576
NdeI CATATG 2 cut(s) 85, 528
NdeII GATC 5 cut(s) 9, 145, 287, 346, 646
NheI GCTAGC 1 cut(s) 202
NlaIII CATG 2 cut(s) 482, 521
NlaIV GGNNCC 1 cut(s) 640
PdmI GAANNNNTTC 1 cut(s) 26
PfeI GAWTC 4 cut(s) 184, 302, 337, 872
PkrI GCNGC 1 cut(s) 384
PleI GAGTC 1 cut(s) 595
PpsI GAGTC 1 cut(s) 595
PspFI CCCAGC 2 cut(s) 31, 206
PspN4I GGNNCC 1 cut(s) 640
PstI CTGCAG 1 cut(s) 269
PsuI RGATCY 1 cut(s) 287
RsaI GTAC 2 cut(s) 256, 419
RsaNI GTAC 2 cut(s) 255, 418
RseI CAYNNNNRTG 2 cut(s) 483, 903
SaqAI TTAA 3 cut(s) 467, 837, 851
SatI GCNGC 1 cut(s) 383
Sau3AI GATC 5 cut(s) 9, 145, 287, 346, 646
SchI GAGTC 1 cut(s) 595
SduI GDGCHC 1 cut(s) 458
SfaNI GCATC 1 cut(s) 480
SfcI CTRYAG 1 cut(s) 265
SmiMI CAYNNNNRTG 2 cut(s) 483, 903
Sse9I AATT 8 cut(s) 323, 397, 532, 565, 610, 713, 930, 1030
SsiI CCGC 2 cut(s) 5, 312
SspI AATATT 1 cut(s) 727
SspMI CTAG 1 cut(s) 203
TaaI ACNGT 3 cut(s) 297, 373, 409
TaqI TCGA 2 cut(s) 913, 995
TasI AATT 8 cut(s) 323, 397, 532, 565, 610, 713, 930, 1030
TatI WGTACW 2 cut(s) 254, 417
TfiI GAWTC 4 cut(s) 184, 302, 337, 872
Tru1I TTAA 3 cut(s) 467, 837, 851
Tru9I TTAA 3 cut(s) 467, 837, 851
TseI GCWGC 1 cut(s) 382
TspDTI ATGAA 9 cut(s) 352, 495, 515, 545, 795, 855, 935, 980, 1043
XapI RAATTY 3 cut(s) 323, 397, 1030
XcmI CCANNNNNNNNNTGG 1 cut(s) 803
XmnI GAANNNNTTC 1 cut(s) 26
XspI CTAG 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.