MD08G1190400.v1.1

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0013874
Physical Location & Seq
Forward (+)
24079319 .. 24080353
1035 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1190400.v1.1.491

Sequence Viewer

Length: 1035 bp
ATGAATTTCCTCAACACTAAAACCTCTTTAATGGCAGTCATCAAACACAGAAAGCTCTTCCCATCTGCACCAATTTCCACAAACCAAACTGATTGTCCAGACTTTTGTGGCCCCGATTGCACGTACGACTGTTACCCTTATGCTGATTTCTACACACCACCACCACCACCAACTTCAGTTATCGATCAAACCAACCAAAACCACCATATCTCACCTTATGTGGTGCTTTTAGTCTCTTTGCTAGCCAGTTTTTTCCTTCTTATTGGGTACTATATTATTGTGGTGAAGTCTTGCACAAGTTGGTGCAGTCACAGAAGTATTGGATCTTCACCCTCTCAATCTACCGGCACGGATGAAGAGTTACTTGCAGATCAAGTAGACCATCCAATCTGGTTCATCTCCTCTGTTGGTTTGCAACAGTCAATCATAAATTCAATTTCAGTTTGTAAGTACAGGAAAGACGAGGGCTTGATCGAAGGGACCGAGTGTTCGGTTTGTTTGAATGAGTTTCGAGAAGATGACACTCTAAGGCTGTTGCCAAAGTGCAATCATGCTTTCCACATTCCTTGTATTGACACTTGGTTGAGGTCTCACACGAATTGCCCGATATGTCGCGCTAATATTGTGAGAGAGACTCAGATCAGTGCTTCCACAGACACAAATGGGGAACCCCAGATGGAGAATTCTGAGAGTGAAGATGAAATAGGTGCAAATAATCAAGTGGGATATCAAGACGTTTGTGAAAACAGAGCTGGGGTGGATAATGAAGGTGAAAATATGCCAAAGCAGGAAATAAATTCAAATGAAAATTGGGTTCTTGAAAGAAGGTCTATCTCAATTATTTCCTCAATGGCTCCAACAAAGTTAGTCATCAAAGTGCAGAATGCTGAGAAAATGCTTTCGGACCTTGCTCGAAAGCGTCGTTCTTCGTTTAGGCAATGTCTACATATAAGCCCTATTTTGATGAAAAGGTCTTTGAGACGTAAGTGGAGCAAAATTTCAGTTGTTCCCCGTAAAGTTTGTTTAATTGTGTGA

Protein Analysis

345

Amino Acids

38.84

Weight (kDa)

6.32

Isoelectric Point (pI)

55.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 162 - 205 8.8e-14 Ring finger domain
zf-RING_11 PF17123 162 - 191 1.3e-10 RING-like zinc finger
zf-C3HC4_2 PF13923 162 - 204 5.7e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 163 - 204 1.7e-07 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 165 - 205 1.4e-08 RING-H2 zinc finger domain
zf-ANAPC11 PF12861 169 - 208 6.8e-06 Anaphase-promoting complex subunit 11 RING-H2 finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013874)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G72220
fragaria_vesca FvH4_5g36252
malus_domestica MD08G1190400.v1.1 MD15G1377700.v1.1
prunus_persica Prupe.1G524400_v2.0.a1
pyrus_communis pycom08g16360 pycom15g33840
rosa_chinensis RchiOBHm_Chr7g0238151
rosa_laevigata RLG00000000938
rosa_multiflora Rmu_sc0008459.1_g000001
rosa_roxburghii Rroxscaffold_3G00223320
rosa_rugosa Rorug07G0310600
rosa_samantha Rh7AG466300 Rh7BG436800 Rh7CG484100 Rh7DG452500
rosa_wichuraiana Rw7G038610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 378, 943
AccII CGCG 1 cut(s) 615
AclWI GGATC 1 cut(s) 331
AcsI RAATTY 5 cut(s) 4, 430, 682, 796, 996
AcuI CTGAAG 1 cut(s) 159
AfaI GTAC 3 cut(s) 125, 269, 452
AgsI TTSAA 4 cut(s) 435, 502, 801, 821
AloI GAACNNNNNNTCC 2 cut(s) 472, 504
AluBI AGCT 2 cut(s) 55, 752
AluI AGCT 2 cut(s) 55, 752
Alw26I GTCTC 4 cut(s) 238, 594, 626, 973
AlwI GGATC 1 cut(s) 331
AoxI GGCC 1 cut(s) 109
ApoI RAATTY 5 cut(s) 4, 430, 682, 796, 996
ArsI GACNNNNNNTTYG 4 cut(s) 79, 111, 472, 504
AspLEI GCGC 1 cut(s) 617
AspS9I GGNCC 3 cut(s) 110, 480, 904
AsuHPI GGTGA 4 cut(s) 204, 295, 321, 782
AsuNHI GCTAGC 1 cut(s) 241
AvaII GGWCC 2 cut(s) 480, 904
BarI GAAGNNNNNNTAC 2 cut(s) 310, 342
BccI CCATC 3 cut(s) 70, 390, 670
BcoDI GTCTC 4 cut(s) 238, 594, 626, 973
BfaI CTAG 1 cut(s) 242
Bme18I GGWCC 2 cut(s) 480, 904
BmgT120I GGNCC 3 cut(s) 110, 480, 904
BmiI GGNNCC 4 cut(s) 112, 481, 669, 855
BmtI GCTAGC 1 cut(s) 245
BplI GAGNNNNNCTC 2 cut(s) 619, 651
Bsa29I ATCGAT 1 cut(s) 183
BsaAI YACGTR 1 cut(s) 123
BsaI GGTCTC 1 cut(s) 594
Bse118I RCCGGY 1 cut(s) 344
Bse1I ACTGG 1 cut(s) 246
Bse3DI GCAATG 1 cut(s) 944
BseCI ATCGAT 1 cut(s) 183
BseGI GGATG 2 cut(s) 358, 382
BseMI GCAATG 1 cut(s) 944
BseMII CTCAG 3 cut(s) 650, 678, 879
BseNI ACTGG 1 cut(s) 246
BseRI GAGGAG 1 cut(s) 391
BseYI CCCAGC 1 cut(s) 752
BsgI GTGCAG 3 cut(s) 51, 325, 899
Bsh1236I CGCG 1 cut(s) 615
BshFI GGCC 1 cut(s) 111
BshVI ATCGAT 1 cut(s) 183
BsiSI CCGG 1 cut(s) 345
BsiWI CGTACG 1 cut(s) 123
BslFI GGGAC 1 cut(s) 493
BsmAI GTCTC 4 cut(s) 238, 594, 626, 973
BsmBI CGTCTC 1 cut(s) 973
BsmFI GGGAC 1 cut(s) 493
BsmI GAATGC 1 cut(s) 889
BsnI GGCC 1 cut(s) 111
Bso31I GGTCTC 1 cut(s) 594
Bsp143I GATC 5 cut(s) 184, 323, 370, 471, 639
BspANI GGCC 1 cut(s) 111
BspCNI CTCAG 3 cut(s) 649, 679, 880
BspDI ATCGAT 1 cut(s) 183
BspFNI CGCG 1 cut(s) 615
BspLI GGNNCC 4 cut(s) 112, 481, 669, 855
BspOI GCTAGC 1 cut(s) 245
BspPI GGATC 1 cut(s) 331
BspQI GCTCTTC 1 cut(s) 62
BspTNI GGTCTC 1 cut(s) 594
BsrDI GCAATG 1 cut(s) 944
BsrFI RCCGGY 1 cut(s) 344
BsrI ACTGG 1 cut(s) 246
BssAI RCCGGY 1 cut(s) 344
BssMI GATC 5 cut(s) 184, 323, 370, 471, 639
Bst4CI ACNGT 2 cut(s) 131, 420
Bst6I CTCTTC 2 cut(s) 62, 351
BstBAI YACGTR 1 cut(s) 123
BstC8I GCNNGC 1 cut(s) 243
BstDEI CTNAG 4 cut(s) 527, 636, 687, 888
BstF5I GGATG 2 cut(s) 358, 382
BstFNI CGCG 1 cut(s) 615
BstHHI GCGC 1 cut(s) 617
BstKTI GATC 5 cut(s) 187, 326, 373, 474, 642
BstMAI GTCTC 4 cut(s) 238, 594, 626, 973
BstMBI GATC 5 cut(s) 184, 323, 370, 471, 639
BstMWI GCNNNNNNNGC 1 cut(s) 117
BstUI CGCG 1 cut(s) 615
BstX2I RGATCY 1 cut(s) 323
BstYI RGATCY 1 cut(s) 323
Bsu15I ATCGAT 1 cut(s) 183
BsuRI GGCC 1 cut(s) 111
BsuTUI ATCGAT 1 cut(s) 183
BtsCI GGATG 2 cut(s) 358, 382
BtsIMutI CAGTG 1 cut(s) 649
Cac8I GCNNGC 1 cut(s) 243
CfoI GCGC 1 cut(s) 617
Cfr10I RCCGGY 1 cut(s) 344
Cfr13I GGNCC 3 cut(s) 110, 480, 904
ClaI ATCGAT 1 cut(s) 183
CseI GACGC 1 cut(s) 908
Csp6I GTAC 3 cut(s) 124, 268, 451
CspCI CAANNNNNGTGG 2 cut(s) 548, 583
CviAII CATG 1 cut(s) 551
CviJI RGCY 8 cut(s) 55, 111, 245, 468, 532, 752, 854, 954
CviKI_1 RGCY 8 cut(s) 55, 111, 245, 468, 532, 752, 854, 954
CviQI GTAC 3 cut(s) 124, 268, 451
DdeI CTNAG 4 cut(s) 527, 636, 687, 888
DpnI GATC 5 cut(s) 186, 325, 372, 473, 641
DpnII GATC 5 cut(s) 184, 323, 370, 471, 639
Eam1104I CTCTTC 2 cut(s) 62, 351
EarI CTCTTC 2 cut(s) 62, 351
Eco31I GGTCTC 1 cut(s) 594
Eco32I GATATC 1 cut(s) 728
Eco47I GGWCC 2 cut(s) 480, 904
Eco57I CTGAAG 1 cut(s) 159
EcoRI GAATTC 1 cut(s) 682
EcoRV GATATC 1 cut(s) 728
Esp3I CGTCTC 1 cut(s) 973
FaeI CATG 1 cut(s) 554
FalI AAGNNNNNCTT 2 cut(s) 348, 380
FaqI GGGAC 1 cut(s) 493
FatI CATG 1 cut(s) 550
FblI GTMKAC 2 cut(s) 378, 943
FokI GGATG 2 cut(s) 365, 369
FspBI CTAG 1 cut(s) 242
GlaI GCGC 1 cut(s) 616
GsaI CCCAGC 1 cut(s) 756
HaeIII GGCC 1 cut(s) 111
HapII CCGG 1 cut(s) 345
HgaI GACGC 1 cut(s) 908
HhaI GCGC 1 cut(s) 617
Hin1II CATG 1 cut(s) 554
Hin6I GCGC 1 cut(s) 615
HinP1I GCGC 1 cut(s) 615
HinfI GANTC 1 cut(s) 634
HpaII CCGG 1 cut(s) 345
HphI GGTGA 4 cut(s) 204, 295, 321, 782
Hpy166II GTNNAC 2 cut(s) 379, 944
Hpy188I TCNGA 3 cut(s) 639, 688, 904
Hpy188III TCNNGA 4 cut(s) 98, 512, 731, 818
Hpy8I GTNNAC 2 cut(s) 379, 944
Hpy99I CGWCG 1 cut(s) 924
HpyAV CCTTC 4 cut(s) 266, 470, 761, 819
HpyCH4III ACNGT 2 cut(s) 131, 420
HpyCH4IV ACGT 3 cut(s) 122, 735, 982
HpyCH4V TGCA 9 cut(s) 68, 120, 294, 306, 368, 415, 546, 710, 880
HpyF10VI GCNNNNNNNGC 1 cut(s) 117
HpyF3I CTNAG 4 cut(s) 527, 636, 687, 888
HpySE526I ACGT 3 cut(s) 122, 735, 982
Hsp92II CATG 1 cut(s) 554
HspAI GCGC 1 cut(s) 615
Kzo9I GATC 5 cut(s) 184, 323, 370, 471, 639
LguI GCTCTTC 1 cut(s) 62
LmnI GCTCC 2 cut(s) 859, 990
LpnPI CCDG 8 cut(s) 111, 259, 358, 376, 439, 686, 738, 773
MaeI CTAG 1 cut(s) 242
MaeII ACGT 3 cut(s) 122, 735, 982
MaeIII GTNAC 3 cut(s) 131, 308, 360
MalI GATC 5 cut(s) 186, 325, 372, 473, 641
MboI GATC 5 cut(s) 184, 323, 370, 471, 639
MboII GAAGA 6 cut(s) 49, 318, 368, 527, 707, 918
MflI RGATCY 1 cut(s) 323
MlyI GAGTC 1 cut(s) 628
MmeI TCCRAC 1 cut(s) 881
MnlI CCTC 7 cut(s) 20, 34, 343, 412, 457, 579, 856
MseI TTAA 2 cut(s) 29, 1025
MslI CAYNNNNRTG 1 cut(s) 875
MspI CCGG 1 cut(s) 345
Mva1269I GAATGC 1 cut(s) 889
MvnI CGCG 1 cut(s) 615
MwoI GCNNNNNNNGC 1 cut(s) 117
NdeII GATC 5 cut(s) 184, 323, 370, 471, 639
NheI GCTAGC 1 cut(s) 241
NlaIII CATG 1 cut(s) 554
NlaIV GGNNCC 4 cut(s) 112, 481, 669, 855
NmuCI GTSAC 1 cut(s) 308
PciSI GCTCTTC 1 cut(s) 62
PcsI WCGNNNNNNNCGW 3 cut(s) 480, 602, 919
PctI GAATGC 1 cut(s) 889
Pfl23II CGTACG 1 cut(s) 123
PleI GAGTC 1 cut(s) 628
PpsI GAGTC 1 cut(s) 628
Ppu21I YACGTR 1 cut(s) 123
PspFI CCCAGC 1 cut(s) 752
PspLI CGTACG 1 cut(s) 123
PspN4I GGNNCC 4 cut(s) 112, 481, 669, 855
PspPI GGNCC 3 cut(s) 110, 480, 904
PsuI RGATCY 1 cut(s) 323
RsaI GTAC 3 cut(s) 125, 269, 452
RsaNI GTAC 3 cut(s) 124, 268, 451
RseI CAYNNNNRTG 1 cut(s) 875
SapI GCTCTTC 1 cut(s) 62
SaqAI TTAA 2 cut(s) 29, 1025
Sau3AI GATC 5 cut(s) 184, 323, 370, 471, 639
Sau96I GGNCC 3 cut(s) 110, 480, 904
SchI GAGTC 1 cut(s) 628
SinI GGWCC 2 cut(s) 480, 904
SmiMI CAYNNNNRTG 1 cut(s) 875
SspI AATATT 1 cut(s) 622
SspMI CTAG 1 cut(s) 242
TaaI ACNGT 2 cut(s) 131, 420
TaiI ACGT 3 cut(s) 125, 738, 985
TaqI TCGA 4 cut(s) 183, 474, 511, 913
TaqII GACCGA 1 cut(s) 497
TatI WGTACW 1 cut(s) 450
Tru1I TTAA 2 cut(s) 29, 1025
Tru9I TTAA 2 cut(s) 29, 1025
TscAI CASTG 1 cut(s) 649
TseFI GTSAC 1 cut(s) 308
Tsp45I GTSAC 1 cut(s) 308
TspDTI ATGAA 7 cut(s) 17, 369, 385, 714, 780, 819, 980
TspGWI ACGGA 1 cut(s) 365
TspRI CASTG 1 cut(s) 649
VpaK11BI GGWCC 2 cut(s) 480, 904
XapI RAATTY 5 cut(s) 4, 430, 682, 796, 996
XmiI GTMKAC 2 cut(s) 378, 943
XspI CTAG 1 cut(s) 242
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.