Rh7BG436800

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0013874
Physical Location & Seq
Reverse (-)
50213255 .. 50220456
7202 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG436800.1

Sequence Viewer

Length: 1113 bp
ATGGCGGTGATCAGACACAGGAAACTCTTCCCAGCACCATTTTCCTCAAACCAAACTGACTGCCCAGACTGCCCAGATTGCACATACGATTGTTACCCCTATACAGATTTCTATATTCCCCCACCACCACCATTTCCTGCCATTGATCAAAGTGACCAAAACCACCATATCTCACCTTATGTGATTCTGTTCCTCTCTTTGCTAGCTGGGCTTTTCCTTCTCATTGCCTACTATGTCATTATAGTAAAGTCCTGTACAAGTTTCTGCAGTCCTAATAGAAACAATGGATCTCCACAGTCCGAATCAACCAGCGGAGATGAGGAATTTCTTGCAGAGAATCAAGTTGATCACCCAATATGGTTCATCACCACCGTTGGATTGCAGCAATCTATCATAAATTCTATCACAGTTTGTAAGTATAAGAAATGTGAGGGCTTGATTGAAGGGACAGAGTGCTCTGTTTGTTTAAATGAGTTTCATGAAGATGAGATGCTTAGATTATTGCCCAAGTGTAGTCATGCTTTTCATATGAACTGTATTGATACTTGGTTGAGGTCTCACACAAATTGCCCTCTATGCCGTGCCTATATTACTTCTGAGACTCTCGGTAATTCGAACATTGTGGGGCTTGATTTGGGTTCCGGAGATCAAAATGGGGATGGCATAGACACAAATGGAGATACCCTGATGGAGATTTCTGAGATTGAAAGTGAATTGGATGTGAATAATCAAGTGGGATATGACAATCAGGTGTGTGAAAACAGAGCTGGAACAGAGGATGACGGTGAAGAACACCAGTTTGGCAATGGAACTACATCAAAAGAGGTAATAACTCTTAATGAAGTTCCATTAAGAAGGTCTATTTCGATGGATTCTTCGTCGGCTGAAACCAAGTTCCATTCAGACGTTGCTCGAAATCGGAAGCCTTCGTTGTTTCCTTCAATCGTCATGTGTGCAAGGAATCTTGATGATTACTGTTCACCGCCGCGAGTCATGGGCTCCATTTTGGGCTCTCGCGGGAATCCCCATTTGGTTTCCCTCGCGGCGCGAGGCGAGTCCTTTCCAGCTTGGGCCGCTAGTTCAGCCCATGGTGCTTGTCCTTCTTGGACCTAG

Protein Analysis

370

Amino Acids

40.84

Weight (kDa)

4.75

Isoelectric Point (pI)

52.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 151 - 194 6.6e-14 Ring finger domain
zf-RING_11 PF17123 151 - 180 2.5e-10 RING-like zinc finger
zf-C3HC4_2 PF13923 151 - 193 1e-07 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 152 - 194 3.1e-09 RING-H2 zinc finger domain
zf-C3HC4 PF00097 152 - 193 1.2e-06 Zinc finger, C3HC4 type (RING finger)
zf-ANAPC11 PF12861 158 - 197 9.1e-06 Anaphase-promoting complex subunit 11 RING-H2 finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013874)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G72220
fragaria_vesca FvH4_5g36252
malus_domestica MD08G1190400.v1.1 MD15G1377700.v1.1
prunus_persica Prupe.1G524400_v2.0.a1
pyrus_communis pycom08g16360 pycom15g33840
rosa_chinensis RchiOBHm_Chr7g0238151
rosa_laevigata RLG00000000938
rosa_multiflora Rmu_sc0008459.1_g000001
rosa_roxburghii Rroxscaffold_3G00223320
rosa_rugosa Rorug07G0310600
rosa_samantha Rh7AG466300 Rh7BG436800 Rh7CG484100 Rh7DG452500
rosa_wichuraiana Rw7G038610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 4 cut(s) 988, 1017, 1043, 1048
AccIII TCCGGA 1 cut(s) 641
AciI CCGC 7 cut(s) 5, 312, 983, 986, 1017, 1043, 1074
AclWI GGATC 1 cut(s) 295
AcsI RAATTY 2 cut(s) 323, 397
AfaI GTAC 1 cut(s) 256
AgsI TTSAA 3 cut(s) 443, 707, 942
AjuI GAANNNNNNNTTGG 4 cut(s) 783, 815, 1013, 1045
AluBI AGCT 3 cut(s) 206, 767, 1067
AluI AGCT 3 cut(s) 206, 767, 1067
Alw21I GWGCWC 1 cut(s) 458
Alw26I GTCTC 2 cut(s) 561, 593
AlwI GGATC 1 cut(s) 295
Aor13HI TCCGGA 1 cut(s) 641
AoxI GGCC 1 cut(s) 1071
ApeKI GCWGC 1 cut(s) 382
ApoI RAATTY 2 cut(s) 323, 397
Asp700I GAANNNNTTC 1 cut(s) 26
AspLEI GCGC 1 cut(s) 1048
AspS9I GGNCC 2 cut(s) 1071, 1107
AsuHPI GGTGA 6 cut(s) 19, 165, 341, 358, 797, 972
AsuII TTCGAA 1 cut(s) 614
AsuNHI GCTAGC 1 cut(s) 202
AvaII GGWCC 1 cut(s) 1107
BanII GRGCYC 2 cut(s) 1001, 1013
Bbv12I GWGCWC 1 cut(s) 458
BbvI GCAGC 1 cut(s) 394
BccI CCATC 3 cut(s) 653, 682, 862
BceAI ACGGC 1 cut(s) 564
BclI TGATCA 3 cut(s) 9, 145, 346
BcoDI GTCTC 2 cut(s) 561, 593
BfaI CTAG 3 cut(s) 203, 1077, 1111
BfmI CTRYAG 1 cut(s) 265
BisI GCNGC 4 cut(s) 383, 986, 1044, 1074
BlsI GCNGC 4 cut(s) 384, 987, 1045, 1075
Bme18I GGWCC 1 cut(s) 1107
BmgT120I GGNCC 2 cut(s) 1071, 1107
BmiI GGNNCC 2 cut(s) 640, 1000
BmsI GCATC 1 cut(s) 480
BmtI GCTAGC 1 cut(s) 206
Bpu14I TTCGAA 1 cut(s) 614
BsaI GGTCTC 1 cut(s) 561
BsaJI CCNNGG 1 cut(s) 1087
BsaWI WCCGGW 1 cut(s) 641
Bse1I ACTGG 1 cut(s) 796
Bse3DI GCAATG 2 cut(s) 222, 811
BseAI TCCGGA 1 cut(s) 641
BseDI CCNNGG 1 cut(s) 1087
BseGI GGATG 3 cut(s) 664, 724, 784
BseMI GCAATG 2 cut(s) 222, 811
BseMII CTCAG 2 cut(s) 588, 690
BseNI ACTGG 1 cut(s) 796
BseXI GCAGC 1 cut(s) 394
BseYI CCCAGC 2 cut(s) 31, 206
Bsh1236I CGCG 4 cut(s) 988, 1017, 1043, 1048
BshFI GGCC 1 cut(s) 1073
BsiHKAI GWGCWC 1 cut(s) 458
BsiSI CCGG 1 cut(s) 642
BslFI GGGAC 1 cut(s) 460
BsmAI GTCTC 2 cut(s) 561, 593
BsmFI GGGAC 1 cut(s) 460
BsnI GGCC 1 cut(s) 1073
Bso31I GGTCTC 1 cut(s) 561
Bsp119I TTCGAA 1 cut(s) 614
Bsp1286I GDGCHC 3 cut(s) 458, 1001, 1013
Bsp13I TCCGGA 1 cut(s) 641
Bsp1407I TGTACA 1 cut(s) 254
Bsp143I GATC 5 cut(s) 9, 145, 287, 346, 646
Bsp19I CCATGG 1 cut(s) 1087
BspACI CCGC 7 cut(s) 5, 312, 983, 986, 1017, 1043, 1074
BspANI GGCC 1 cut(s) 1073
BspCNI CTCAG 2 cut(s) 589, 691
BspEI TCCGGA 1 cut(s) 641
BspFNI CGCG 4 cut(s) 988, 1017, 1043, 1048
BspHI TCATGA 1 cut(s) 478
BspLI GGNNCC 2 cut(s) 640, 1000
BspMAI CTGCAG 1 cut(s) 269
BspOI GCTAGC 1 cut(s) 206
BspPI GGATC 1 cut(s) 295
BspT104I TTCGAA 1 cut(s) 614
BspTNI GGTCTC 1 cut(s) 561
BsrDI GCAATG 2 cut(s) 222, 811
BsrGI TGTACA 1 cut(s) 254
BsrI ACTGG 1 cut(s) 796
BssECI CCNNGG 1 cut(s) 1087
BssMI GATC 5 cut(s) 9, 145, 287, 346, 646
BssT1I CCWWGG 1 cut(s) 1087
Bst4CI ACNGT 6 cut(s) 297, 373, 409, 536, 785, 977
Bst6I CTCTTC 1 cut(s) 32
BstAUI TGTACA 1 cut(s) 254
BstBI TTCGAA 1 cut(s) 614
BstC8I GCNNGC 1 cut(s) 204
BstDEI CTNAG 3 cut(s) 494, 597, 699
BstDSI CCRYGG 1 cut(s) 1087
BstF5I GGATG 3 cut(s) 664, 724, 784
BstFNI CGCG 4 cut(s) 988, 1017, 1043, 1048
BstHHI GCGC 1 cut(s) 1048
BstKTI GATC 5 cut(s) 12, 148, 290, 349, 649
BstMAI GTCTC 2 cut(s) 561, 593
BstMBI GATC 5 cut(s) 9, 145, 287, 346, 646
BstMWI GCNNNNNNNGC 7 cut(s) 69, 78, 208, 576, 1073, 1082, 1091
BstSFI CTRYAG 1 cut(s) 265
BstUI CGCG 4 cut(s) 988, 1017, 1043, 1048
BstV1I GCAGC 1 cut(s) 394
BstX2I RGATCY 1 cut(s) 287
BstYI RGATCY 1 cut(s) 287
BsuRI GGCC 1 cut(s) 1073
BtgI CCRYGG 1 cut(s) 1087
BtsCI GGATG 3 cut(s) 664, 724, 784
Cac8I GCNNGC 1 cut(s) 204
CciI TCATGA 1 cut(s) 478
CfoI GCGC 1 cut(s) 1048
Cfr13I GGNCC 2 cut(s) 1071, 1107
Csp6I GTAC 1 cut(s) 255
CviAII CATG 5 cut(s) 479, 518, 949, 994, 1088
CviQI GTAC 1 cut(s) 255
DdeI CTNAG 3 cut(s) 494, 597, 699
DpnI GATC 5 cut(s) 11, 147, 289, 348, 648
DpnII GATC 5 cut(s) 9, 145, 287, 346, 646
DraI TTTAAA 1 cut(s) 468
Eam1104I CTCTTC 1 cut(s) 32
EarI CTCTTC 1 cut(s) 32
Eco130I CCWWGG 1 cut(s) 1087
Eco24I GRGCYC 2 cut(s) 1001, 1013
Eco31I GGTCTC 1 cut(s) 561
Eco47I GGWCC 1 cut(s) 1107
EcoT14I CCWWGG 1 cut(s) 1087
EcoT38I GRGCYC 2 cut(s) 1001, 1013
ErhI CCWWGG 1 cut(s) 1087
FaeI CATG 5 cut(s) 482, 521, 952, 997, 1091
FaqI GGGAC 1 cut(s) 460
FatI CATG 5 cut(s) 478, 517, 948, 993, 1087
FauI CCCGC 1 cut(s) 1010
FauNDI CATATG 1 cut(s) 528
FbaI TGATCA 3 cut(s) 9, 145, 346
Fnu4HI GCNGC 4 cut(s) 383, 986, 1044, 1074
FokI GGATG 3 cut(s) 671, 731, 791
FriOI GRGCYC 2 cut(s) 1001, 1013
Fsp4HI GCNGC 4 cut(s) 383, 986, 1044, 1074
FspBI CTAG 3 cut(s) 203, 1077, 1111
GlaI GCGC 1 cut(s) 1047
GluI GCNGC 4 cut(s) 383, 986, 1044, 1074
GsaI CCCAGC 2 cut(s) 35, 210
HaeIII GGCC 1 cut(s) 1073
HapII CCGG 1 cut(s) 642
HhaI GCGC 1 cut(s) 1048
Hin1II CATG 5 cut(s) 482, 521, 952, 997, 1091
Hin6I GCGC 1 cut(s) 1046
HinP1I GCGC 1 cut(s) 1046
HinfI GANTC 9 cut(s) 184, 302, 337, 601, 872, 961, 990, 1021, 1055
HpaII CCGG 1 cut(s) 642
HphI GGTGA 6 cut(s) 19, 165, 341, 358, 797, 972
Hpy166II GTNNAC 1 cut(s) 980
Hpy188I TCNGA 6 cut(s) 14, 301, 598, 700, 904, 921
Hpy188III TCNNGA 3 cut(s) 479, 642, 965
Hpy8I GTNNAC 1 cut(s) 980
Hpy99I CGWCG 1 cut(s) 883
HpyAV CCTTC 6 cut(s) 227, 437, 849, 936, 948, 1110
HpyCH4III ACNGT 6 cut(s) 297, 373, 409, 536, 785, 977
HpyCH4IV ACGT 1 cut(s) 906
HpyCH4V TGCA 5 cut(s) 81, 267, 332, 382, 956
HpyF10VI GCNNNNNNNGC 7 cut(s) 69, 78, 208, 576, 1073, 1082, 1091
HpyF3I CTNAG 3 cut(s) 494, 597, 699
HpySE526I ACGT 1 cut(s) 906
Hsp92II CATG 5 cut(s) 482, 521, 952, 997, 1091
HspAI GCGC 1 cut(s) 1046
Kpn2I TCCGGA 1 cut(s) 641
Ksp22I TGATCA 3 cut(s) 9, 145, 346
Kzo9I GATC 5 cut(s) 9, 145, 287, 346, 646
LmnI GCTCC 1 cut(s) 1004
Lsp1109I GCAGC 1 cut(s) 394
LweI GCATC 1 cut(s) 480
MaeI CTAG 3 cut(s) 203, 1077, 1111
MaeII ACGT 1 cut(s) 906
MaeIII GTNAC 2 cut(s) 92, 152
MalI GATC 5 cut(s) 11, 147, 289, 348, 648
MboI GATC 5 cut(s) 9, 145, 287, 346, 646
MboII GAAGA 4 cut(s) 19, 494, 800, 867
MflI RGATCY 1 cut(s) 287
MhlI GDGCHC 3 cut(s) 458, 1001, 1013
MluCI AATT 5 cut(s) 323, 397, 565, 610, 713
MlyI GAGTC 3 cut(s) 595, 999, 1064
MmeI TCCRAC 1 cut(s) 355
MroI TCCGGA 1 cut(s) 641
MroXI GAANNNNTTC 1 cut(s) 26
MseI TTAA 3 cut(s) 467, 837, 851
MslI CAYNNNNRTG 1 cut(s) 483
MspA1I CMGCKG 1 cut(s) 312
MspI CCGG 1 cut(s) 642
MvnI CGCG 4 cut(s) 988, 1017, 1043, 1048
MwoI GCNNNNNNNGC 7 cut(s) 69, 78, 208, 576, 1073, 1082, 1091
NcoI CCATGG 1 cut(s) 1087
NdeI CATATG 1 cut(s) 528
NdeII GATC 5 cut(s) 9, 145, 287, 346, 646
NheI GCTAGC 1 cut(s) 202
NlaIII CATG 5 cut(s) 482, 521, 952, 997, 1091
NlaIV GGNNCC 2 cut(s) 640, 1000
NmuCI GTSAC 1 cut(s) 152
NspV TTCGAA 1 cut(s) 614
PagI TCATGA 1 cut(s) 478
PdmI GAANNNNTTC 1 cut(s) 26
PfeI GAWTC 6 cut(s) 184, 302, 337, 872, 961, 1021
PkrI GCNGC 4 cut(s) 384, 987, 1045, 1075
PleI GAGTC 3 cut(s) 595, 998, 1063
PpsI GAGTC 3 cut(s) 595, 998, 1063
PspFI CCCAGC 2 cut(s) 31, 206
PspN4I GGNNCC 2 cut(s) 640, 1000
PspPI GGNCC 2 cut(s) 1071, 1107
PstI CTGCAG 1 cut(s) 269
PsuI RGATCY 1 cut(s) 287
RsaI GTAC 1 cut(s) 256
RsaNI GTAC 1 cut(s) 255
RseI CAYNNNNRTG 1 cut(s) 483
SaqAI TTAA 3 cut(s) 467, 837, 851
SatI GCNGC 4 cut(s) 383, 986, 1044, 1074
Sau3AI GATC 5 cut(s) 9, 145, 287, 346, 646
Sau96I GGNCC 2 cut(s) 1071, 1107
SchI GAGTC 3 cut(s) 595, 999, 1064
SduI GDGCHC 3 cut(s) 458, 1001, 1013
SfaNI GCATC 1 cut(s) 480
SfcI CTRYAG 1 cut(s) 265
SfuI TTCGAA 1 cut(s) 614
SinI GGWCC 1 cut(s) 1107
SmiMI CAYNNNNRTG 1 cut(s) 483
Sse9I AATT 5 cut(s) 323, 397, 565, 610, 713
SsiI CCGC 7 cut(s) 5, 312, 983, 986, 1017, 1043, 1074
SspMI CTAG 3 cut(s) 203, 1077, 1111
StyI CCWWGG 1 cut(s) 1087
TaaI ACNGT 6 cut(s) 297, 373, 409, 536, 785, 977
TaiI ACGT 1 cut(s) 909
TaqI TCGA 3 cut(s) 614, 866, 913
TasI AATT 5 cut(s) 323, 397, 565, 610, 713
TatI WGTACW 1 cut(s) 254
TauI GCSGC 3 cut(s) 988, 1046, 1076
TfiI GAWTC 6 cut(s) 184, 302, 337, 872, 961, 1021
Tru1I TTAA 3 cut(s) 467, 837, 851
Tru9I TTAA 3 cut(s) 467, 837, 851
TseFI GTSAC 1 cut(s) 152
TseI GCWGC 1 cut(s) 382
Tsp45I GTSAC 1 cut(s) 152
TspDTI ATGAA 6 cut(s) 352, 467, 495, 515, 545, 855
VpaK11BI GGWCC 1 cut(s) 1107
XapI RAATTY 2 cut(s) 323, 397
XcmI CCANNNNNNNNNTGG 1 cut(s) 803
XmnI GAANNNNTTC 1 cut(s) 26
XspI CTAG 3 cut(s) 203, 1077, 1111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.