pycom08g16360

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0013874
Physical Location & Seq
Forward (+)
16270388 .. 16271089
702 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g16360.1

Sequence Viewer

Length: 702 bp
ATGGATCGATCTTCACCCTCTCAATCTACCGGCACGGATGAAGAGTTACTTGAAGATCAAGTAGACCATCCAATCTGGTTCATCTCCTCCGTTGGTTTGCAACAGTCAATCATAAATTCAATTTCAGTTTGTAAGTACAGGAAAGACGAGGGCTTGATCGAAGGGACTGAGTGTTCGGTTTGTTTGAATGAGTTTCGAGAAGATGACACCCTAAGGCTGTTGCCAAAGTGCAATCATGCTTTCCACATTCCTTGTATTGACACTTGGTTGAGGTCCCACACGAATTGCCCGATATGCCGCGCTAATATTATGAGAGAGACTCAGATCAGTACTTCCACAGACACAAATGGAGAACCCCAGATGGAGAATTCTGAGAGTGAAGGTGAAATAGGTGCAAATAATCAAGTGGGATATCAAGACGTTTGCGAAAACAGAGCTGAGGTGGATGATGACGGTGAAAATATGCCAAAGCAGGAAATAAATTCAAATGAAAATTGGGCTCTTGAAAGAAGGTCTATCTCAATTATTTCCTCATTGGCTCCAACAAAGTTAGTCATCAAAGTGCAGAATGCTGACAAAATGCATTCGGACCTTGCTCGAAAGCGTCATTCTCCCATTAGGCAATGTCTACATATAAGCCCTATTTTGATGAAACGGTCTTTGAGACGTAAGTGGAGCAAAATTTCTGTTGTTCCCCTGTAA

Protein Analysis

234

Amino Acids

26.46

Weight (kDa)

5.42

Isoelectric Point (pI)

62.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 57 - 100 4.8e-14 Ring finger domain
zf-RING_11 PF17123 57 - 86 7.6e-11 RING-like zinc finger
zf-C3HC4_2 PF13923 57 - 99 3.2e-08 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 58 - 100 1.2e-08 RING-H2 zinc finger domain
zf-C3HC4 PF00097 58 - 99 9.3e-08 Zinc finger, C3HC4 type (RING finger)
zf-ANAPC11 PF12861 64 - 103 4.1e-06 Anaphase-promoting complex subunit 11 RING-H2 finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013874)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G72220
fragaria_vesca FvH4_5g36252
malus_domestica MD08G1190400.v1.1 MD15G1377700.v1.1
prunus_persica Prupe.1G524400_v2.0.a1
pyrus_communis pycom08g16360 pycom15g33840
rosa_chinensis RchiOBHm_Chr7g0238151
rosa_laevigata RLG00000000938
rosa_multiflora Rmu_sc0008459.1_g000001
rosa_roxburghii Rroxscaffold_3G00223320
rosa_rugosa Rorug07G0310600
rosa_samantha Rh7AG466300 Rh7BG436800 Rh7CG484100 Rh7DG452500
rosa_wichuraiana Rw7G038610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 63, 628
AccII CGCG 1 cut(s) 300
AciI CCGC 1 cut(s) 298
AclWI GGATC 1 cut(s) 12
AcsI RAATTY 4 cut(s) 115, 367, 481, 681
AfaI GTAC 2 cut(s) 137, 331
AgsI TTSAA 5 cut(s) 53, 120, 187, 486, 506
AloI GAACNNNNNNTCC 2 cut(s) 157, 189
AluBI AGCT 1 cut(s) 437
AluI AGCT 1 cut(s) 437
Alw26I GTCTC 2 cut(s) 311, 658
AlwI GGATC 1 cut(s) 12
ApoI RAATTY 4 cut(s) 115, 367, 481, 681
ArsI GACNNNNNNTTYG 2 cut(s) 157, 189
AspLEI GCGC 1 cut(s) 302
AspS9I GGNCC 2 cut(s) 273, 589
AsuHPI GGTGA 3 cut(s) 6, 395, 467
AvaII GGWCC 2 cut(s) 273, 589
AxyI CCTNAGG 1 cut(s) 212
BanII GRGCYC 1 cut(s) 502
BbvCI CCTCAGC 1 cut(s) 438
BccI CCATC 2 cut(s) 75, 355
BcoDI GTCTC 2 cut(s) 311, 658
BisI GCNGC 1 cut(s) 298
BlsI GCNGC 1 cut(s) 299
BmcAI AGTACT 1 cut(s) 331
Bme18I GGWCC 2 cut(s) 273, 589
BmgT120I GGNCC 2 cut(s) 273, 589
BmiI GGNNCC 2 cut(s) 275, 540
BplI GAGNNNNNCTC 2 cut(s) 304, 336
Bpu10I CCTNAGC 1 cut(s) 438
Bsa29I ATCGAT 1 cut(s) 7
Bse118I RCCGGY 1 cut(s) 29
Bse21I CCTNAGG 1 cut(s) 212
Bse3DI GCAATG 1 cut(s) 629
BseCI ATCGAT 1 cut(s) 7
BseGI GGATG 3 cut(s) 43, 67, 451
BseMI GCAATG 1 cut(s) 629
BseMII CTCAG 4 cut(s) 159, 335, 363, 429
BseRI GAGGAG 1 cut(s) 76
BsgI GTGCAG 1 cut(s) 584
Bsh1236I CGCG 1 cut(s) 300
BshVI ATCGAT 1 cut(s) 7
BsiSI CCGG 1 cut(s) 30
BslFI GGGAC 2 cut(s) 178, 259
BsmAI GTCTC 2 cut(s) 311, 658
BsmBI CGTCTC 1 cut(s) 658
BsmFI GGGAC 2 cut(s) 178, 259
BsmI GAATGC 2 cut(s) 574, 583
Bsp1286I GDGCHC 1 cut(s) 502
Bsp143I GATC 5 cut(s) 4, 8, 55, 156, 324
BspACI CCGC 1 cut(s) 298
BspCNI CTCAG 4 cut(s) 160, 334, 364, 430
BspDI ATCGAT 1 cut(s) 7
BspFNI CGCG 1 cut(s) 300
BspLI GGNNCC 2 cut(s) 275, 540
BspPI GGATC 1 cut(s) 12
BsrDI GCAATG 1 cut(s) 629
BsrFI RCCGGY 1 cut(s) 29
BssAI RCCGGY 1 cut(s) 29
BssMI GATC 5 cut(s) 4, 8, 55, 156, 324
Bst4CI ACNGT 3 cut(s) 105, 455, 657
Bst6I CTCTTC 1 cut(s) 36
BstDEI CTNAG 5 cut(s) 168, 212, 321, 372, 438
BstF5I GGATG 3 cut(s) 43, 67, 451
BstFNI CGCG 1 cut(s) 300
BstHHI GCGC 1 cut(s) 302
BstKTI GATC 5 cut(s) 7, 11, 58, 159, 327
BstMAI GTCTC 2 cut(s) 311, 658
BstMBI GATC 5 cut(s) 4, 8, 55, 156, 324
BstMWI GCNNNNNNNGC 1 cut(s) 294
BstUI CGCG 1 cut(s) 300
Bsu15I ATCGAT 1 cut(s) 7
Bsu36I CCTNAGG 1 cut(s) 212
BsuTUI ATCGAT 1 cut(s) 7
BtsCI GGATG 3 cut(s) 43, 67, 451
CfoI GCGC 1 cut(s) 302
Cfr10I RCCGGY 1 cut(s) 29
Cfr13I GGNCC 2 cut(s) 273, 589
ClaI ATCGAT 1 cut(s) 7
CseI GACGC 1 cut(s) 593
Csp6I GTAC 2 cut(s) 136, 330
CspCI CAANNNNNGTGG 4 cut(s) 233, 266, 268, 301
CviAII CATG 1 cut(s) 236
CviJI RGCY 6 cut(s) 153, 217, 437, 500, 539, 639
CviKI_1 RGCY 6 cut(s) 153, 217, 437, 500, 539, 639
CviQI GTAC 2 cut(s) 136, 330
DdeI CTNAG 5 cut(s) 168, 212, 321, 372, 438
DpnI GATC 5 cut(s) 6, 10, 57, 158, 326
DpnII GATC 5 cut(s) 4, 8, 55, 156, 324
Eam1104I CTCTTC 1 cut(s) 36
EarI CTCTTC 1 cut(s) 36
Eco24I GRGCYC 1 cut(s) 502
Eco32I GATATC 1 cut(s) 413
Eco47I GGWCC 2 cut(s) 273, 589
Eco81I CCTNAGG 1 cut(s) 212
EcoO109I RGGNCCY 1 cut(s) 273
EcoRI GAATTC 1 cut(s) 367
EcoRV GATATC 1 cut(s) 413
EcoT22I ATGCAT 1 cut(s) 585
EcoT38I GRGCYC 1 cut(s) 502
Esp3I CGTCTC 1 cut(s) 658
FaeI CATG 1 cut(s) 239
FaiI YATR 7 cut(s) 113, 237, 295, 311, 464, 633, 635
FalI AAGNNNNNCTT 2 cut(s) 33, 65
FaqI GGGAC 2 cut(s) 178, 259
FatI CATG 1 cut(s) 235
FblI GTMKAC 2 cut(s) 63, 628
Fnu4HI GCNGC 1 cut(s) 298
FokI GGATG 3 cut(s) 50, 54, 458
FriOI GRGCYC 1 cut(s) 502
Fsp4HI GCNGC 1 cut(s) 298
GlaI GCGC 1 cut(s) 301
GluI GCNGC 1 cut(s) 298
HapII CCGG 1 cut(s) 30
HgaI GACGC 1 cut(s) 593
HhaI GCGC 1 cut(s) 302
Hin1II CATG 1 cut(s) 239
Hin6I GCGC 1 cut(s) 300
HinP1I GCGC 1 cut(s) 300
HinfI GANTC 1 cut(s) 319
HpaII CCGG 1 cut(s) 30
HphI GGTGA 3 cut(s) 6, 395, 467
Hpy166II GTNNAC 2 cut(s) 64, 629
Hpy188I TCNGA 3 cut(s) 324, 373, 589
Hpy188III TCNNGA 3 cut(s) 197, 416, 503
Hpy8I GTNNAC 2 cut(s) 64, 629
HpyAV CCTTC 3 cut(s) 155, 374, 504
HpyCH4III ACNGT 3 cut(s) 105, 455, 657
HpyCH4IV ACGT 2 cut(s) 420, 667
HpyCH4V TGCA 5 cut(s) 100, 231, 395, 565, 583
HpyF10VI GCNNNNNNNGC 1 cut(s) 294
HpyF3I CTNAG 5 cut(s) 168, 212, 321, 372, 438
HpySE526I ACGT 2 cut(s) 420, 667
Hsp92II CATG 1 cut(s) 239
HspAI GCGC 1 cut(s) 300
Kzo9I GATC 5 cut(s) 4, 8, 55, 156, 324
LmnI GCTCC 2 cut(s) 544, 675
LpnPI CCDG 5 cut(s) 43, 61, 124, 371, 458
MaeII ACGT 2 cut(s) 420, 667
MaeIII GTNAC 1 cut(s) 45
MalI GATC 5 cut(s) 6, 10, 57, 158, 326
MboI GATC 5 cut(s) 4, 8, 55, 156, 324
MboII GAAGA 4 cut(s) 3, 53, 65, 212
MhlI GDGCHC 1 cut(s) 502
MluCI AATT 8 cut(s) 115, 120, 283, 367, 481, 493, 522, 681
MlyI GAGTC 1 cut(s) 313
MmeI TCCRAC 1 cut(s) 566
MnlI CCTC 6 cut(s) 28, 97, 142, 264, 433, 541
Mph1103I ATGCAT 1 cut(s) 585
MslI CAYNNNNRTG 1 cut(s) 560
MspI CCGG 1 cut(s) 30
Mva1269I GAATGC 2 cut(s) 574, 583
MvnI CGCG 1 cut(s) 300
MwoI GCNNNNNNNGC 1 cut(s) 294
NdeII GATC 5 cut(s) 4, 8, 55, 156, 324
NlaIII CATG 1 cut(s) 239
NlaIV GGNNCC 2 cut(s) 275, 540
NsiI ATGCAT 1 cut(s) 585
PcsI WCGNNNNNNNCGW 1 cut(s) 287
PctI GAATGC 2 cut(s) 574, 583
PkrI GCNGC 1 cut(s) 299
PleI GAGTC 1 cut(s) 313
PpsI GAGTC 1 cut(s) 313
PpuMI RGGWCCY 1 cut(s) 273
Psp5II RGGWCCY 1 cut(s) 273
PspN4I GGNNCC 2 cut(s) 275, 540
PspPI GGNCC 2 cut(s) 273, 589
PspPPI RGGWCCY 1 cut(s) 273
RsaI GTAC 2 cut(s) 137, 331
RsaNI GTAC 2 cut(s) 136, 330
RseI CAYNNNNRTG 1 cut(s) 560
SatI GCNGC 1 cut(s) 298
Sau3AI GATC 5 cut(s) 4, 8, 55, 156, 324
Sau96I GGNCC 2 cut(s) 273, 589
ScaI AGTACT 1 cut(s) 331
SchI GAGTC 1 cut(s) 313
SduI GDGCHC 1 cut(s) 502
SetI ASST 9 cut(s) 275, 385, 394, 423, 439, 444, 515, 594, 670
SinI GGWCC 2 cut(s) 273, 589
SmiMI CAYNNNNRTG 1 cut(s) 560
Sse9I AATT 8 cut(s) 115, 120, 283, 367, 481, 493, 522, 681
SsiI CCGC 1 cut(s) 298
SspI AATATT 1 cut(s) 307
TaaI ACNGT 3 cut(s) 105, 455, 657
TaiI ACGT 2 cut(s) 423, 670
TaqI TCGA 4 cut(s) 7, 159, 196, 598
TasI AATT 8 cut(s) 115, 120, 283, 367, 481, 493, 522, 681
TatI WGTACW 2 cut(s) 135, 329
TauI GCSGC 1 cut(s) 300
TspDTI ATGAA 4 cut(s) 54, 70, 504, 665
TspGWI ACGGA 2 cut(s) 50, 79
VpaK11BI GGWCC 2 cut(s) 273, 589
XapI RAATTY 4 cut(s) 115, 367, 481, 681
XmiI GTMKAC 2 cut(s) 63, 628
ZrmI AGTACT 1 cut(s) 331
Zsp2I ATGCAT 1 cut(s) 585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.