FvH4_5g38470

Pentatricopeptide repeat-containing protein At1g19720-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0012631
Physical Location & Seq
Forward (+)
28442825 .. 28446071
3247 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g38470.t1

Sequence Viewer

Length: 1797 bp
ATGCCTGAGAGAACTGTACCCACTTATGCGGCTTTGATTAGGGCGTATTGTCGGTCCGAGCAGTGGGAGGACTTGTTTGTGGTGTTTGGGTTGATGGTTGAGGAGGGAATGGTGCCGGATATGTATGTTGTGCCTACATTGTTGAAGGCATGTGCGCTGACGAAGATGGTGGGGATTGGGAGGATGATTCATGGGTTTGTGATAAGGAAGGAGATGGATTTGGATGTTTTTGTGGGGAACTCGCTTGTAGATTTTTATGCCAACTGTGGGGATTTGGGGTTTGCAGTGAGTGTGTTTGGTGCAATGAGGGAAAGAGATGTGGTCTCGTGGACTGCACTTGTTTCGGCTTATATGAATGAGGGTCTTTGTGAGGAAGCTTTGGAGGTTTTCAGTTTGATGAAGGTGAATGGGGTGAAGAGGGATTTGATATCTTGGAATGTGCTTGTTTCGGGGTTTGCAAAGAATGGGGAGATTGAAATGGCTCTGCAGTATTTGGAGGCGATGCAAGAGGAAGGGTTAAGGCCGAGAGTTAATTCTTGGAATGGAGTCATTTCGGGGTGTATTCAGAATGAATATTTTGAGGATGCTTTGGATGCATTTTATAGAATGTTGTGTTTCCTCGAGGAGCCAAATTTTGTTACAATTGCAAGCATTTTACCAGCTTGTGCAGGCTTGAAAGATCTGAACTTGGGCAGGGCTATTCATGGATTTATTCTGAAGCGTCAGCTTTGCGATAACACTCATGTGGAAGGTTCATTGATTGGTATGTACTCAAAATGTGGGTCGAAAGATTATGCAGAGAAATTTTTTTCCATGACCAAAACCAAAAGCATTGGAATGTGGAATGAGATGATTGCAGCTTATGTGAATTCAGGAGAAACAGAGAAGGGAATAGAGCTTCTAAGGGTGATGCATCATCATGGCTTAAAACCTGATGTGGTATCCTATAATACAATATTGGCTGGCCATGCAAGAAATGGTCAAAAGAATGAGGCATATGAATTGCTTCATGAGTTGGTCCAGATGGATTTGAAACCAAATATTGTCTCTTTCAACGTCTTAATTTCTGGTTTTCAACAATTTGGGCTTAGTTTTGAAGCTTTGAAATTGTTCTGGACCATGCAATCCCCATGTGATGGTTGCTTTGCTGATTTTGTGCTCCCTGAGTCGATCCAACCAAATTCCATTACGGTTGCTGGTGCTCTTGCAGCTTGTGCTGATCTCAAACTGCTATCCCAGGGGAAGGAAATCCATGGATATACGCTGAGGTGTGGGTTTGAGCGTAATGTCTATATCTCAAGTGCATTGGTTGACATGTATTCGAAATGTCTGGATATTGACTCAGCTACGGAAGTATTTAGAAGAACTGAGGACAGAAACACAATCTGTTGGAATTCTATGATTGCAGGCCATGTCAACAACATGCAGCTGAACTTAGCTCTTGAACTTTTCTGCGATATGCTAGAAGAAGGTGTTGAACCAAGCTCAATAACTCTCATGATACTTCTCCTCACTTGTGGTAATATGACAGCTTTGAGATTTGGAAGAGCATTACATGGCTATATCATAAAGACTCAGCTTGATGAGTCTGATAGTACCCTTGCAAGTGCCTTAGTAGGCATGTTTGCTAAGTGTGGTAGTATCAAGGAAGCTAAATCAGTGTTTGACTTTGAAGTTAAAAGAGATGCTTTGCTGTGGAATGCCATGCTATCTGCTTATTCAACTAATGGAATGCTCAACAATGCCCTTGCCTTGGTTGGAGAAATGGAGTTAGTGGGCGTCGTGCCTGAGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2 PPR_3

Protein Analysis

599

Amino Acids

66.48

Weight (kDa)

4.98

Isoelectric Point (pI)

35.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 5 - 52 1.1e-06 PPR repeat family
PPR_2 PF13041 105 - 141 9.7e-07 PPR repeat family
PPR_3 PF13812 106 - 150 7.9e-06 Pentatricopeptide repeat domain
PPR PF01535 108 - 138 2.6e-07 PPR repeat
PPR_3 PF13812 133 - 187 6.1e-06 Pentatricopeptide repeat domain
PPR PF01535 143 - 173 6.2e-07 PPR repeat
PPR_2 PF13041 278 - 318 1.8e-07 PPR repeat family
TPR_24 PF23276 282 - 371 3.3e-06 Fungal tetratrico peptide repeats
PPR_3 PF13812 300 - 356 8e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 308 - 338 8.7e-08 PPR repeat
PPR_2 PF13041 311 - 358 1.3e-13 PPR repeat family
PPR_2 PF13041 460 - 505 1.5e-10 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 620
AccB1I GGYRCC 1 cut(s) 112
AccB7I CCANNNNNTGG 1 cut(s) 1136
AciI CCGC 1 cut(s) 29
AclWI GGATC 1 cut(s) 1165
AcoI YGGCCR 1 cut(s) 964
AcsI RAATTY 5 cut(s) 631, 803, 868, 1180, 1393
AcuI CTGAAG 1 cut(s) 737
AcyI GRCGYC 1 cut(s) 1779
AfaI GTAC 3 cut(s) 18, 770, 1597
AfiI CCNNNNNNNGG 5 cut(s) 63, 267, 1136, 1243, 1753
AflIII ACRYGT 1 cut(s) 1314
AjnI CCWGG 1 cut(s) 1236
AleI CACNNNNGTG 1 cut(s) 743
Alw21I GWGCWC 2 cut(s) 1161, 1204
Alw26I GTCTC 2 cut(s) 328, 1051
AlwI GGATC 1 cut(s) 1165
Ama87I CYCGRG 1 cut(s) 620
AoxI GGCC 3 cut(s) 521, 964, 1408
ApeKI GCWGC 3 cut(s) 857, 1208, 1426
ApoI RAATTY 5 cut(s) 631, 803, 868, 1180, 1393
ArsI GACNNNNNNTTYG 2 cut(s) 767, 799
Asp700I GAANNNNTTC 2 cut(s) 1005, 1109
AspLEI GCGC 1 cut(s) 157
AspS9I GGNCC 3 cut(s) 54, 1018, 1116
AsuHPI GGTGA 3 cut(s) 415, 424, 919
AsuII TTCGAA 1 cut(s) 1322
AvaI CYCGRG 1 cut(s) 620
AvaII GGWCC 3 cut(s) 54, 1018, 1116
BalI TGGCCA 1 cut(s) 966
BanI GGYRCC 1 cut(s) 112
BauI CACGAG 1 cut(s) 325
Bbv12I GWGCWC 2 cut(s) 1161, 1204
BbvCI CCTCAGC 1 cut(s) 1265
BbvI GCAGC 3 cut(s) 869, 1220, 1438
BccI CCATC 5 cut(s) 88, 160, 208, 1018, 1130
BcgI CGANNNNNNTGC 2 cut(s) 324, 358
BciT130I CCWGG 1 cut(s) 1238
BciVI GTATCC 1 cut(s) 952
BcoDI GTCTC 2 cut(s) 328, 1051
BfaI CTAG 1 cut(s) 1463
BfmI CTRYAG 1 cut(s) 485
BfuI GTATCC 1 cut(s) 952
BglII AGATCT 1 cut(s) 679
BisI GCNGC 4 cut(s) 30, 858, 1209, 1427
BlsI GCNGC 4 cut(s) 31, 859, 1210, 1428
Bme1390I CCNGG 1 cut(s) 1238
Bme18I GGWCC 3 cut(s) 54, 1018, 1116
BmeT110I CYCGRG 1 cut(s) 620
BmgT120I GGNCC 3 cut(s) 54, 1018, 1116
BmiI GGNNCC 2 cut(s) 114, 627
BmrFI CCNGG 1 cut(s) 1238
BmsI GCATC 6 cut(s) 492, 574, 583, 900, 922, 1675
Bpu10I CCTNAGC 1 cut(s) 1265
Bpu14I TTCGAA 1 cut(s) 1322
BpuEI CTTGAG 1 cut(s) 1282
BsaHI GRCGYC 1 cut(s) 1779
BsaI GGTCTC 1 cut(s) 328
BsaJI CCNNGG 4 cut(s) 1236, 1237, 1252, 1752
BsaXI ACNNNNNCTCC 2 cut(s) 59, 89
Bsc4I CCNNNNNNNGG 5 cut(s) 63, 267, 1136, 1243, 1753
Bse3DI GCAATG 1 cut(s) 309
BseBI CCWGG 1 cut(s) 1238
BseDI CCNNGG 4 cut(s) 1236, 1237, 1252, 1752
BseGI GGATG 4 cut(s) 189, 229, 589, 598
BseLI CCNNNNNNNGG 5 cut(s) 63, 267, 1136, 1243, 1753
BseMI GCAATG 1 cut(s) 309
BseMII CTCAG 6 cut(s) 1155, 1256, 1356, 1359, 1589, 1779
BseRI GAGGAG 3 cut(s) 116, 638, 1499
BseXI GCAGC 3 cut(s) 869, 1220, 1438
BsgI GTGCAG 2 cut(s) 318, 687
BshFI GGCC 3 cut(s) 523, 966, 1410
BshNI GGYRCC 1 cut(s) 112
BsiHKAI GWGCWC 2 cut(s) 1161, 1204
BsiHKCI CYCGRG 1 cut(s) 620
BsiSI CCGG 1 cut(s) 116
BslI CCNNNNNNNGG 5 cut(s) 63, 267, 1136, 1243, 1753
BsmAI GTCTC 2 cut(s) 328, 1051
BsmI GAATGC 2 cut(s) 1705, 1737
BsnI GGCC 3 cut(s) 523, 966, 1410
Bso31I GGTCTC 1 cut(s) 328
BsoBI CYCGRG 1 cut(s) 620
Bsp119I TTCGAA 1 cut(s) 1322
Bsp1286I GDGCHC 2 cut(s) 1161, 1204
Bsp143I GATC 3 cut(s) 679, 1170, 1219
Bsp19I CCATGG 1 cut(s) 1252
BspACI CCGC 1 cut(s) 29
BspANI GGCC 3 cut(s) 523, 966, 1410
BspCNI CTCAG 6 cut(s) 1156, 1257, 1355, 1360, 1588, 1780
BspHI TCATGA 2 cut(s) 1009, 1497
BspLI GGNNCC 2 cut(s) 114, 627
BspMAI CTGCAG 1 cut(s) 489
BspPI GGATC 1 cut(s) 1165
BspQI GCTCTTC 1 cut(s) 1540
BspT104I TTCGAA 1 cut(s) 1322
BspT107I GGYRCC 1 cut(s) 112
BspTNI GGTCTC 1 cut(s) 328
BsrDI GCAATG 1 cut(s) 309
BssECI CCNNGG 4 cut(s) 1236, 1237, 1252, 1752
BssMI GATC 3 cut(s) 679, 1170, 1219
BssNI GRCGYC 1 cut(s) 1779
BssSI CACGAG 1 cut(s) 325
BssT1I CCWWGG 2 cut(s) 1252, 1752
Bst2BI CACGAG 1 cut(s) 325
Bst2UI CCWGG 1 cut(s) 1238
Bst4CI ACNGT 3 cut(s) 16, 266, 1192
Bst6I CTCTTC 2 cut(s) 410, 1540
BstACI GRCGYC 1 cut(s) 1779
BstAPI GCANNNNNTGC 1 cut(s) 1214
BstBI TTCGAA 1 cut(s) 1322
BstC8I GCNNGC 4 cut(s) 649, 670, 964, 1408
BstDSI CCRYGG 1 cut(s) 1252
BstF5I GGATG 4 cut(s) 189, 229, 589, 598
BstHHI GCGC 1 cut(s) 157
BstKTI GATC 3 cut(s) 682, 1173, 1222
BstMAI GTCTC 2 cut(s) 328, 1051
BstMBI GATC 3 cut(s) 679, 1170, 1219
BstMWI GCNNNNNNNGC 4 cut(s) 593, 968, 1208, 1214
BstNI CCWGG 1 cut(s) 1238
BstNSI RCATGY 4 cut(s) 153, 1318, 1426, 1624
BstSCI CCNGG 1 cut(s) 1236
BstSFI CTRYAG 1 cut(s) 485
BstV1I GCAGC 3 cut(s) 869, 1220, 1438
BstX2I RGATCY 1 cut(s) 679
BstYI RGATCY 1 cut(s) 679
BsuI GTATCC 1 cut(s) 952
BsuRI GGCC 3 cut(s) 523, 966, 1410
BtgI CCRYGG 1 cut(s) 1252
BtgZI GCGATG 1 cut(s) 515
BtsCI GGATG 4 cut(s) 189, 229, 589, 598
BtsI GCAGTG 2 cut(s) 68, 291
BtsIMutI CAGTG 3 cut(s) 68, 291, 1665
Cac8I GCNNGC 4 cut(s) 649, 670, 964, 1408
CciI TCATGA 2 cut(s) 1009, 1497
CfoI GCGC 1 cut(s) 157
Cfr13I GGNCC 3 cut(s) 54, 1018, 1116
CpoI CGGWCCG 1 cut(s) 54
CseI GACGC 2 cut(s) 710, 1768
Csp6I GTAC 3 cut(s) 17, 769, 1596
CspI CGGWCCG 1 cut(s) 54
CviQI GTAC 3 cut(s) 17, 769, 1596
DpnI GATC 3 cut(s) 681, 1172, 1221
DpnII GATC 3 cut(s) 679, 1170, 1219
EaeI YGGCCR 1 cut(s) 964
Eam1104I CTCTTC 2 cut(s) 410, 1540
EarI CTCTTC 2 cut(s) 410, 1540
Eco130I CCWWGG 2 cut(s) 1252, 1752
Eco31I GGTCTC 1 cut(s) 328
Eco32I GATATC 1 cut(s) 429
Eco47I GGWCC 3 cut(s) 54, 1018, 1116
Eco57I CTGAAG 1 cut(s) 737
Eco88I CYCGRG 1 cut(s) 620
EcoRI GAATTC 2 cut(s) 868, 1393
EcoRII CCWGG 1 cut(s) 1236
EcoRV GATATC 1 cut(s) 429
EcoT14I CCWWGG 2 cut(s) 1252, 1752
EcoT22I ATGCAT 2 cut(s) 598, 915
ErhI CCWWGG 2 cut(s) 1252, 1752
FalI AAGNNNNNCTT 2 cut(s) 1672, 1704
FauNDI CATATG 1 cut(s) 997
Fnu4HI GCNGC 4 cut(s) 30, 858, 1209, 1427
FokI GGATG 4 cut(s) 196, 236, 596, 605
Fsp4HI GCNGC 4 cut(s) 30, 858, 1209, 1427
FspBI CTAG 1 cut(s) 1463
GlaI GCGC 1 cut(s) 156
GluI GCNGC 4 cut(s) 30, 858, 1209, 1427
HaeIII GGCC 3 cut(s) 523, 966, 1410
HapII CCGG 1 cut(s) 116
HgaI GACGC 2 cut(s) 710, 1768
HhaI GCGC 1 cut(s) 157
Hin1I GRCGYC 1 cut(s) 1779
Hin6I GCGC 1 cut(s) 155
HinP1I GCGC 1 cut(s) 155
HincII GTYRAC 2 cut(s) 1312, 1417
HindII GTYRAC 2 cut(s) 1312, 1417
HindIII AAGCTT 2 cut(s) 375, 1098
HinfI GANTC 6 cut(s) 187, 546, 1166, 1340, 1573, 1586
HpaII CCGG 1 cut(s) 116
HphI GGTGA 3 cut(s) 415, 424, 919
Hpy166II GTNNAC 3 cut(s) 330, 1312, 1417
Hpy188I TCNGA 5 cut(s) 58, 567, 684, 717, 1591
Hpy188III TCNNGA 7 cut(s) 873, 1010, 1021, 1114, 1331, 1442, 1498
Hpy8I GTNNAC 3 cut(s) 330, 1312, 1417
Hpy99I CGWCG 1 cut(s) 1784
HpyAV CCTTC 8 cut(s) 139, 202, 394, 506, 743, 880, 1237, 1463
HpyCH4III ACNGT 3 cut(s) 16, 266, 1192
HpyCH4IV ACGT 1 cut(s) 1056
HpyF10VI GCNNNNNNNGC 4 cut(s) 593, 968, 1208, 1214
HpySE526I ACGT 1 cut(s) 1056
Hsp92I GRCGYC 1 cut(s) 1779
HspAI GCGC 1 cut(s) 155
Kzo9I GATC 3 cut(s) 679, 1170, 1219
LguI GCTCTTC 1 cut(s) 1540
LmnI GCTCC 2 cut(s) 625, 1164
Lsp1109I GCAGC 3 cut(s) 869, 1220, 1438
LweI GCATC 6 cut(s) 492, 574, 583, 900, 922, 1675
MaeI CTAG 1 cut(s) 1463
MaeII ACGT 1 cut(s) 1056
MaeIII GTNAC 1 cut(s) 637
MalI GATC 3 cut(s) 681, 1172, 1221
MboI GATC 3 cut(s) 679, 1170, 1219
MboII GAAGA 5 cut(s) 175, 427, 1374, 1478, 1557
MfeI CAATTG 1 cut(s) 642
MflI RGATCY 1 cut(s) 679
MhlI GDGCHC 2 cut(s) 1161, 1204
MlsI TGGCCA 1 cut(s) 966
MluNI TGGCCA 1 cut(s) 966
MlyI GAGTC 5 cut(s) 555, 1175, 1334, 1567, 1595
MmeI TCCRAC 3 cut(s) 1198, 1370, 1738
Mox20I TGGCCA 1 cut(s) 966
Mph1103I ATGCAT 2 cut(s) 598, 915
MroXI GAANNNNTTC 2 cut(s) 1005, 1109
MscI TGGCCA 1 cut(s) 966
MseI TTAA 5 cut(s) 518, 531, 926, 1061, 1677
MslI CAYNNNNRTG 3 cut(s) 743, 836, 918
Msp20I TGGCCA 1 cut(s) 966
MspA1I CMGCKG 1 cut(s) 1429
MspI CCGG 1 cut(s) 116
MspR9I CCNGG 1 cut(s) 1238
MunI CAATTG 1 cut(s) 642
Mva1269I GAATGC 2 cut(s) 1705, 1737
MvaI CCWGG 1 cut(s) 1238
MwoI GCNNNNNNNGC 4 cut(s) 593, 968, 1208, 1214
NcoI CCATGG 1 cut(s) 1252
NdeI CATATG 1 cut(s) 997
NdeII GATC 3 cut(s) 679, 1170, 1219
NlaIV GGNNCC 2 cut(s) 114, 627
NmeAIII GCCGAG 1 cut(s) 549
NsiI ATGCAT 2 cut(s) 598, 915
NspI RCATGY 4 cut(s) 153, 1318, 1426, 1624
NspV TTCGAA 1 cut(s) 1322
OliI CACNNNNGTG 1 cut(s) 743
PaeR7I CTCGAG 1 cut(s) 620
PagI TCATGA 2 cut(s) 1009, 1497
PasI CCCWGGG 1 cut(s) 1237
PciI ACATGT 1 cut(s) 1314
PciSI GCTCTTC 1 cut(s) 1540
PctI GAATGC 2 cut(s) 1705, 1737
PdmI GAANNNNTTC 2 cut(s) 1005, 1109
PfeI GAWTC 1 cut(s) 187
PflMI CCANNNNNTGG 1 cut(s) 1136
PkrI GCNGC 4 cut(s) 31, 859, 1210, 1428
PleI GAGTC 5 cut(s) 554, 1174, 1334, 1567, 1594
PpsI GAGTC 5 cut(s) 554, 1174, 1334, 1567, 1594
PscI ACATGT 1 cut(s) 1314
Psp6I CCWGG 1 cut(s) 1236
PspGI CCWGG 1 cut(s) 1236
PspN4I GGNNCC 2 cut(s) 114, 627
PspPI GGNCC 3 cut(s) 54, 1018, 1116
PspXI VCTCGAGB 1 cut(s) 620
PstI CTGCAG 1 cut(s) 489
PsuI RGATCY 1 cut(s) 679
PvuII CAGCTG 1 cut(s) 1429
RsaI GTAC 3 cut(s) 18, 770, 1597
RsaNI GTAC 3 cut(s) 17, 769, 1596
RseI CAYNNNNRTG 3 cut(s) 743, 836, 918
Rsr2I CGGWCCG 1 cut(s) 54
RsrII CGGWCCG 1 cut(s) 54
SapI GCTCTTC 1 cut(s) 1540
SaqAI TTAA 5 cut(s) 518, 531, 926, 1061, 1677
SatI GCNGC 4 cut(s) 30, 858, 1209, 1427
Sau3AI GATC 3 cut(s) 679, 1170, 1219
Sau96I GGNCC 3 cut(s) 54, 1018, 1116
SchI GAGTC 5 cut(s) 555, 1175, 1334, 1567, 1595
ScrFI CCNGG 1 cut(s) 1238
SduI GDGCHC 2 cut(s) 1161, 1204
SfaNI GCATC 6 cut(s) 492, 574, 583, 900, 922, 1675
SfcI CTRYAG 1 cut(s) 485
Sfr274I CTCGAG 1 cut(s) 620
SfuI TTCGAA 1 cut(s) 1322
SinI GGWCC 3 cut(s) 54, 1018, 1116
SlaI CTCGAG 1 cut(s) 620
SmiMI CAYNNNNRTG 3 cut(s) 743, 836, 918
SmlI CTYRAG 2 cut(s) 620, 1297
SmoI CTYRAG 2 cut(s) 620, 1297
SsiI CCGC 1 cut(s) 29
SspI AATATT 3 cut(s) 575, 957, 1042
SspMI CTAG 1 cut(s) 1463
StyD4I CCNGG 1 cut(s) 1236
StyI CCWWGG 2 cut(s) 1252, 1752
TaaI ACNGT 3 cut(s) 16, 266, 1192
TaiI ACGT 1 cut(s) 1059
TaqI TCGA 4 cut(s) 621, 785, 1169, 1322
TaqII GACCGA 1 cut(s) 42
TatI WGTACW 1 cut(s) 768
TauI GCSGC 1 cut(s) 32
TfiI GAWTC 1 cut(s) 187
Tru1I TTAA 5 cut(s) 518, 531, 926, 1061, 1677
Tru9I TTAA 5 cut(s) 518, 531, 926, 1061, 1677
TscAI CASTG 3 cut(s) 68, 291, 1665
TseI GCWGC 3 cut(s) 857, 1208, 1426
TspDTI ATGAA 8 cut(s) 179, 368, 413, 585, 692, 744, 998, 1014
TspGWI ACGGA 1 cut(s) 1364
TspRI CASTG 3 cut(s) 68, 291, 1665
Van91I CCANNNNNTGG 1 cut(s) 1136
VpaK11BI GGWCC 3 cut(s) 54, 1018, 1116
XapI RAATTY 5 cut(s) 631, 803, 868, 1180, 1393
XceI RCATGY 4 cut(s) 153, 1318, 1426, 1624
XcmI CCANNNNNNNNNTGG 1 cut(s) 974
XhoI CTCGAG 1 cut(s) 620
XmnI GAANNNNTTC 2 cut(s) 1005, 1109
XspI CTAG 1 cut(s) 1463
Zsp2I ATGCAT 2 cut(s) 598, 915
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.