Rw7G041160

Pentatricopeptide repeat-containing protein At1g19720-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0012631
Physical Location & Seq
Forward (+)
64107246 .. 64109040
1795 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G041160.1

Sequence Viewer

Length: 1734 bp
ATGCCTAAGAGAACTGTACCCACTTACGCTGCTTTGATTAGTGCGTATTGTCGTGCCGAGCAATGGGAGGATCTGTTCTTGGTGTTTAGGTTGATGGTTGATGAGGGAATGGTGCCTGATGTGTATGTTGTGCCTACACTTTTGAAAGCATGTGCGTTGACGAAAATGGTTGGGATTGGGAGAATGATTCATGCGTTTGTGATGAGGAAGGAGATGGATTCGGATGTTTTTGTGGGTAATTCGCTTATAGATTTTTATGCCAACTGTGGGGATTTGGGAGTTGCGGTGAGTGTGTTTGGCGCAATGAGGGAAAGAGATGTGGTTTCGTGGACTGCCCTTGTTTCGGCTTTTATGAATGAGGGTCTTTTTGAGGAAGCGATGGAGGTTTTTGATTCCATGAAGGTGAATGGGGTGAAGAGGGATTTGATATCTTGGAATGCTCTTGTTTCGGGGTTTGCACAGAATGGGGAGATTGATTTGGCTTTCGGTATTTGGAGAGTTAATTCTTGGAATGGAGTCATTTCGGGCTGTATTCAGAATGAATATTTTGAGGATGCTTTGGATGCATTTTATAAAATGTTGTGTTTCCCCGAGGAGCCAAATTTTGTTACAATTGCAAGCATTTTACCAGCTTGTGCAGGCTTGAAAGAGCTGAACTTAGGCAGGGCAATTCATGGATTTTCTCTGAAGCGTCAGCTTTGCGGTAATACTCATGTGGAAGGTTCATTGATTGGTATGTACTCAAAATGTGGGATGAAAGATTATGCAGGGAAAGTTTTTTCCATGGCTGAGACCAAAAGCATTGGAATGTGGAATGAGATGATTGCAGCTTATGTGAATTCAGGAGATACAGAGAAGGGAATAGAGCTTCTAAGGGTGATGCATCATCATGGCTTAAAACCTGATGTGGTGTCCTATAACACAATATTGGCTGGCCATGCAAGAAATGGGCAAAAGAATGAGGCATATGAGTTGCTTAATGAGATGGTCCGGATGGATTTGAAACCAAATATTATCTCTTTCAACGTTTTAATTTCTGGTTTTCAACAATTTGGGCTTAGTTTTGAAGCTTTGAAATTGTTCTGTACCATGCAATTCCCATCTGATCGTTCCTTTGCTGATTTTGTGCTCCCTGAGTCAATTCAACCAAATTCCATTACTATTGCTGGTGCTCTTGCAGCTGAAATCCATGGATACACGCTGAGGAGTGGCTTTGAGCCTAATGTCTATATCTCAAGTGCATTGGTTGACATGTACTCGAAATGTCTTGATATTGTCTCAGCAACGAATGTATTTAGGAGAATTGAGGACAGAAACACAATCTGTTGGAATGCTATGATCTCAGGCCATGTCAACAACATGCAGCTGAACTTAGCTCTTGAACTTTTCTGCGAAATGCTGGAAGAAGGTCTTGAACCAAGCTCAATTACACTGATGATACTTCTCCTCACTTGTGGTGATATGACAGCTCTGAGATTTGGAAGAGAATTACATGGATATATCATAAAGAGTCAGCTTGATCAGTCTGATAGTACGCTTGCAAGTGCCTTGGTAGGCATGTATGCTAAGTGTGGTAGTATCAAGGAAGCTAAATCAGTGTTTGACTTGGACGTTAAAAAAGATGCCGTGCTGTGGAATGCCATGTTATCTGCTTATTCAACTAATGGAATGCTGAACAATGCCCTTGCCTTGGTTGGACAAATGGAGTTGGTGGGCGTTGTGCCTGAGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2 PPR_3

Protein Analysis

577

Amino Acids

63.88

Weight (kDa)

5.05

Isoelectric Point (pI)

34.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 5 - 52 2.3e-07 PPR repeat family
PPR_2 PF13041 105 - 153 5.9e-11 PPR repeat family
PPR_3 PF13812 106 - 152 2.5e-06 Pentatricopeptide repeat domain
PPR PF01535 108 - 138 1.9e-07 PPR repeat
PPR_2 PF13041 268 - 308 1.2e-07 PPR repeat family
TPR_24 PF23276 271 - 361 1.8e-06 Fungal tetratrico peptide repeats
PPR_3 PF13812 290 - 346 1.1e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 298 - 330 9e-10 PPR repeat
PPR_2 PF13041 301 - 348 1.1e-15 PPR repeat family
PPR PF01535 304 - 333 4.7e-06 PPR repeat
PPR_2 PF13041 439 - 483 1.1e-10 PPR repeat family
PPR PF01535 442 - 471 1.7e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 573
AccB1I GGYRCC 1 cut(s) 112
AccIII TCCGGA 1 cut(s) 990
AciI CCGC 2 cut(s) 284, 702
AclI AACGTT 1 cut(s) 1026
AclWI GGATC 1 cut(s) 78
AcoI YGGCCR 1 cut(s) 934
AcsI RAATTY 3 cut(s) 601, 838, 1150
AcuI CTGAAG 1 cut(s) 707
AfaI GTAC 5 cut(s) 18, 740, 1087, 1256, 1534
AfiI CCNNNNNNNGG 5 cut(s) 63, 267, 343, 1632, 1690
AflIII ACRYGT 1 cut(s) 1251
AloI GAACNNNNNNTCC 2 cut(s) 59, 91
Alw21I GWGCWC 2 cut(s) 1131, 1174
Alw26I GTCTC 2 cut(s) 785, 1282
AlwI GGATC 1 cut(s) 78
Ama87I CYCGRG 1 cut(s) 590
Aor13HI TCCGGA 1 cut(s) 990
AoxI GGCC 2 cut(s) 934, 1345
ApeKI GCWGC 4 cut(s) 29, 827, 1178, 1363
ApoI RAATTY 3 cut(s) 601, 838, 1150
Asp700I GAANNNNTTC 1 cut(s) 1079
AspLEI GCGC 1 cut(s) 302
AspS9I GGNCC 1 cut(s) 988
AsuHPI GGTGA 5 cut(s) 298, 415, 424, 889, 1469
AvaI CYCGRG 1 cut(s) 590
AvaII GGWCC 1 cut(s) 988
BalI TGGCCA 1 cut(s) 936
BanI GGYRCC 1 cut(s) 112
Bbv12I GWGCWC 2 cut(s) 1131, 1174
BbvCI CCTCAGC 1 cut(s) 1202
BbvI GCAGC 4 cut(s) 16, 839, 1190, 1375
BccI CCATC 6 cut(s) 88, 208, 373, 979, 988, 1108
BceAI ACGGC 1 cut(s) 1610
BciVI GTATCC 1 cut(s) 1187
BclI TGATCA 1 cut(s) 1519
BcoDI GTCTC 2 cut(s) 785, 1282
BfuI GTATCC 1 cut(s) 1187
BisI GCNGC 4 cut(s) 30, 828, 1179, 1364
BlsI GCNGC 4 cut(s) 31, 829, 1180, 1365
Bme18I GGWCC 1 cut(s) 988
BmeT110I CYCGRG 1 cut(s) 590
BmgT120I GGNCC 1 cut(s) 988
BmiI GGNNCC 2 cut(s) 114, 597
BmsI GCATC 5 cut(s) 544, 553, 870, 892, 1612
Bpu10I CCTNAGC 1 cut(s) 1202
BpuEI CTTGAG 1 cut(s) 1219
BsaI GGTCTC 1 cut(s) 785
BsaJI CCNNGG 5 cut(s) 591, 783, 1189, 1548, 1689
BsaWI WCCGGW 1 cut(s) 990
BsaXI ACNNNNNCTCC 4 cut(s) 59, 89, 270, 300
Bsc4I CCNNNNNNNGG 5 cut(s) 63, 267, 343, 1632, 1690
Bse3DI GCAATG 2 cut(s) 68, 309
BseAI TCCGGA 1 cut(s) 990
BseDI CCNNGG 5 cut(s) 591, 783, 1189, 1548, 1689
BseGI GGATG 5 cut(s) 229, 559, 568, 759, 999
BseLI CCNNNNNNNGG 5 cut(s) 63, 267, 343, 1632, 1690
BseMI GCAATG 2 cut(s) 68, 309
BseMII CTCAG 7 cut(s) 780, 1125, 1193, 1293, 1356, 1463, 1716
BseRI GAGGAG 3 cut(s) 608, 1219, 1436
BseXI GCAGC 4 cut(s) 16, 839, 1190, 1375
BsgI GTGCAG 1 cut(s) 657
BshFI GGCC 2 cut(s) 936, 1347
BshNI GGYRCC 1 cut(s) 112
BsiHKAI GWGCWC 2 cut(s) 1131, 1174
BsiHKCI CYCGRG 1 cut(s) 590
BsiSI CCGG 1 cut(s) 991
BslI CCNNNNNNNGG 5 cut(s) 63, 267, 343, 1632, 1690
BsmAI GTCTC 2 cut(s) 785, 1282
BsmI GAATGC 4 cut(s) 442, 1336, 1642, 1674
BsnI GGCC 2 cut(s) 936, 1347
Bso31I GGTCTC 1 cut(s) 785
BsoBI CYCGRG 1 cut(s) 590
Bsp1286I GDGCHC 2 cut(s) 1131, 1174
Bsp13I TCCGGA 1 cut(s) 990
Bsp143I GATC 4 cut(s) 70, 1105, 1338, 1519
Bsp19I CCATGG 2 cut(s) 783, 1189
BspACI CCGC 2 cut(s) 284, 702
BspANI GGCC 2 cut(s) 936, 1347
BspCNI CTCAG 7 cut(s) 781, 1126, 1194, 1292, 1355, 1464, 1717
BspEI TCCGGA 1 cut(s) 990
BspLI GGNNCC 2 cut(s) 114, 597
BspPI GGATC 1 cut(s) 78
BspT107I GGYRCC 1 cut(s) 112
BspTNI GGTCTC 1 cut(s) 785
BsrDI GCAATG 2 cut(s) 68, 309
BssECI CCNNGG 5 cut(s) 591, 783, 1189, 1548, 1689
BssMI GATC 4 cut(s) 70, 1105, 1338, 1519
BssT1I CCWWGG 4 cut(s) 783, 1189, 1548, 1689
Bst4CI ACNGT 2 cut(s) 16, 266
Bst6I CTCTTC 2 cut(s) 410, 1477
BstC8I GCNNGC 4 cut(s) 619, 640, 934, 1539
BstDSI CCRYGG 2 cut(s) 783, 1189
BstF5I GGATG 5 cut(s) 229, 559, 568, 759, 999
BstHHI GCGC 1 cut(s) 302
BstKTI GATC 4 cut(s) 73, 1108, 1341, 1522
BstMAI GTCTC 2 cut(s) 785, 1282
BstMBI GATC 4 cut(s) 70, 1105, 1338, 1519
BstMWI GCNNNNNNNGC 3 cut(s) 563, 938, 1178
BstNSI RCATGY 4 cut(s) 153, 1255, 1363, 1561
BstV1I GCAGC 4 cut(s) 16, 839, 1190, 1375
BstX2I RGATCY 1 cut(s) 70
BstYI RGATCY 1 cut(s) 70
BsuI GTATCC 1 cut(s) 1187
BsuRI GGCC 2 cut(s) 936, 1347
BtgI CCRYGG 2 cut(s) 783, 1189
BtgZI GCGATG 1 cut(s) 392
BtsCI GGATG 5 cut(s) 229, 559, 568, 759, 999
BtsIMutI CAGTG 2 cut(s) 1430, 1602
Cac8I GCNNGC 4 cut(s) 619, 640, 934, 1539
CfoI GCGC 1 cut(s) 302
Cfr13I GGNCC 1 cut(s) 988
CseI GACGC 1 cut(s) 680
Csp6I GTAC 5 cut(s) 17, 739, 1086, 1255, 1533
CviQI GTAC 5 cut(s) 17, 739, 1086, 1255, 1533
DpnI GATC 4 cut(s) 72, 1107, 1340, 1521
DpnII GATC 4 cut(s) 70, 1105, 1338, 1519
EaeI YGGCCR 1 cut(s) 934
Eam1104I CTCTTC 2 cut(s) 410, 1477
EarI CTCTTC 2 cut(s) 410, 1477
Eco130I CCWWGG 4 cut(s) 783, 1189, 1548, 1689
Eco31I GGTCTC 1 cut(s) 785
Eco32I GATATC 1 cut(s) 429
Eco47I GGWCC 1 cut(s) 988
Eco57I CTGAAG 1 cut(s) 707
Eco88I CYCGRG 1 cut(s) 590
EcoRI GAATTC 1 cut(s) 838
EcoRV GATATC 1 cut(s) 429
EcoT14I CCWWGG 4 cut(s) 783, 1189, 1548, 1689
EcoT22I ATGCAT 2 cut(s) 568, 885
ErhI CCWWGG 4 cut(s) 783, 1189, 1548, 1689
FalI AAGNNNNNCTT 2 cut(s) 1395, 1427
FauNDI CATATG 1 cut(s) 967
FbaI TGATCA 1 cut(s) 1519
Fnu4HI GCNGC 4 cut(s) 30, 828, 1179, 1364
FokI GGATG 5 cut(s) 236, 566, 575, 766, 1006
Fsp4HI GCNGC 4 cut(s) 30, 828, 1179, 1364
GlaI GCGC 1 cut(s) 301
GluI GCNGC 4 cut(s) 30, 828, 1179, 1364
HaeIII GGCC 2 cut(s) 936, 1347
HapII CCGG 1 cut(s) 991
HgaI GACGC 1 cut(s) 680
HhaI GCGC 1 cut(s) 302
Hin6I GCGC 1 cut(s) 300
HinP1I GCGC 1 cut(s) 300
HincII GTYRAC 3 cut(s) 159, 1249, 1354
HindII GTYRAC 3 cut(s) 159, 1249, 1354
HindIII AAGCTT 1 cut(s) 1068
HinfI GANTC 6 cut(s) 187, 218, 392, 516, 1136, 1510
HpaII CCGG 1 cut(s) 991
HphI GGTGA 5 cut(s) 298, 415, 424, 889, 1469
Hpy166II GTNNAC 4 cut(s) 159, 330, 1249, 1354
Hpy188I TCNGA 6 cut(s) 223, 537, 687, 1105, 1473, 1528
Hpy188III TCNNGA 5 cut(s) 843, 991, 1268, 1379, 1412
Hpy8I GTNNAC 4 cut(s) 159, 330, 1249, 1354
HpyAV CCTTC 5 cut(s) 202, 394, 713, 850, 1400
HpyCH4III ACNGT 2 cut(s) 16, 266
HpyCH4IV ACGT 2 cut(s) 1026, 1611
HpyF10VI GCNNNNNNNGC 3 cut(s) 563, 938, 1178
HpySE526I ACGT 2 cut(s) 1026, 1611
HspAI GCGC 1 cut(s) 300
Kpn2I TCCGGA 1 cut(s) 990
Ksp22I TGATCA 1 cut(s) 1519
Kzo9I GATC 4 cut(s) 70, 1105, 1338, 1519
LmnI GCTCC 2 cut(s) 595, 1134
Lsp1109I GCAGC 4 cut(s) 16, 839, 1190, 1375
LweI GCATC 5 cut(s) 544, 553, 870, 892, 1612
MaeII ACGT 2 cut(s) 1026, 1611
MaeIII GTNAC 1 cut(s) 607
MalI GATC 4 cut(s) 72, 1107, 1340, 1521
MboI GATC 4 cut(s) 70, 1105, 1338, 1519
MboII GAAGA 3 cut(s) 427, 1415, 1494
MfeI CAATTG 1 cut(s) 612
MflI RGATCY 1 cut(s) 70
MhlI GDGCHC 2 cut(s) 1131, 1174
MlsI TGGCCA 1 cut(s) 936
MluNI TGGCCA 1 cut(s) 936
MlyI GAGTC 3 cut(s) 525, 1145, 1519
MmeI TCCRAC 2 cut(s) 1307, 1675
Mox20I TGGCCA 1 cut(s) 936
Mph1103I ATGCAT 2 cut(s) 568, 885
MroI TCCGGA 1 cut(s) 990
MroXI GAANNNNTTC 1 cut(s) 1079
MscI TGGCCA 1 cut(s) 936
MseI TTAA 5 cut(s) 501, 896, 978, 1031, 1614
MslI CAYNNNNRTG 3 cut(s) 401, 806, 888
Msp20I TGGCCA 1 cut(s) 936
MspA1I CMGCKG 2 cut(s) 1181, 1366
MspI CCGG 1 cut(s) 991
MunI CAATTG 1 cut(s) 612
Mva1269I GAATGC 4 cut(s) 442, 1336, 1642, 1674
MwoI GCNNNNNNNGC 3 cut(s) 563, 938, 1178
NcoI CCATGG 2 cut(s) 783, 1189
NdeI CATATG 1 cut(s) 967
NdeII GATC 4 cut(s) 70, 1105, 1338, 1519
NlaIV GGNNCC 2 cut(s) 114, 597
NmeAIII GCCGAG 1 cut(s) 82
NsiI ATGCAT 2 cut(s) 568, 885
NspI RCATGY 4 cut(s) 153, 1255, 1363, 1561
PciI ACATGT 1 cut(s) 1251
PctI GAATGC 4 cut(s) 442, 1336, 1642, 1674
PdmI GAANNNNTTC 1 cut(s) 1079
PfeI GAWTC 3 cut(s) 187, 218, 392
PkrI GCNGC 4 cut(s) 31, 829, 1180, 1365
PleI GAGTC 3 cut(s) 524, 1144, 1518
PpsI GAGTC 3 cut(s) 524, 1144, 1518
PscI ACATGT 1 cut(s) 1251
PsiI TTATAA 1 cut(s) 573
Psp1406I AACGTT 1 cut(s) 1026
PspN4I GGNNCC 2 cut(s) 114, 597
PspPI GGNCC 1 cut(s) 988
PsuI RGATCY 1 cut(s) 70
PvuII CAGCTG 2 cut(s) 1181, 1366
RsaI GTAC 5 cut(s) 18, 740, 1087, 1256, 1534
RsaNI GTAC 5 cut(s) 17, 739, 1086, 1255, 1533
RseI CAYNNNNRTG 3 cut(s) 401, 806, 888
SaqAI TTAA 5 cut(s) 501, 896, 978, 1031, 1614
SatI GCNGC 4 cut(s) 30, 828, 1179, 1364
Sau3AI GATC 4 cut(s) 70, 1105, 1338, 1519
Sau96I GGNCC 1 cut(s) 988
SchI GAGTC 3 cut(s) 525, 1145, 1519
SduI GDGCHC 2 cut(s) 1131, 1174
SfaNI GCATC 5 cut(s) 544, 553, 870, 892, 1612
SinI GGWCC 1 cut(s) 988
SmiMI CAYNNNNRTG 3 cut(s) 401, 806, 888
SmlI CTYRAG 1 cut(s) 1234
SmoI CTYRAG 1 cut(s) 1234
SsiI CCGC 2 cut(s) 284, 702
SspI AATATT 3 cut(s) 545, 927, 1012
StyI CCWWGG 4 cut(s) 783, 1189, 1548, 1689
TaaI ACNGT 2 cut(s) 16, 266
TaiI ACGT 2 cut(s) 1029, 1614
TaqI TCGA 1 cut(s) 1259
TatI WGTACW 2 cut(s) 738, 1254
TfiI GAWTC 3 cut(s) 187, 218, 392
Tru1I TTAA 5 cut(s) 501, 896, 978, 1031, 1614
Tru9I TTAA 5 cut(s) 501, 896, 978, 1031, 1614
TscAI CASTG 2 cut(s) 1437, 1602
TseI GCWGC 4 cut(s) 29, 827, 1178, 1363
TspDTI ATGAA 7 cut(s) 179, 368, 413, 555, 662, 714, 770
TspRI CASTG 2 cut(s) 1437, 1602
VpaK11BI GGWCC 1 cut(s) 988
XapI RAATTY 3 cut(s) 601, 838, 1150
XceI RCATGY 4 cut(s) 153, 1255, 1363, 1561
XcmI CCANNNNNNNNNTGG 1 cut(s) 944
XmnI GAANNNNTTC 1 cut(s) 1079
Zsp2I ATGCAT 2 cut(s) 568, 885
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.