FvH4_5g39190

CP12

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0009967
Physical Location & Seq
Forward (+)
28924463 .. 28925391
929 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g39190.t1

Sequence Viewer

Length: 384 bp
ATGGCAAGTGTAGCAGGAGTGAGCAGCCTCTCGACCCCTGGAGTCTTTGCCAGGGCGGCAGAGACACCCAAGGTGATAAAGCCAGCTGGGGTGAGCTTCGGGTACTCGTTCGGGCAAGTGGGTTCGGGTCGGATGAGGATGATCCGACCGGTGGCAGCTGCTCCGGATAAACTATCGGATAAGGTGCGGGAGAGCATCAAGGAGGCTGAGCAGGCGTGCTCGGAGGACCCGGCCAGCGGGGAGTGCGTGGCGGCGTGGGATGAGGTTGAGGAGCTGAGCGCGGCGGCGAGCCACAAGCGTGACAACGAGAAGAACAAGGACCCCTTGGAGACTTACTGCAACGACAACCCTGAAACCGACGAGTGCCGTACTTACGACAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CP12

Protein Analysis

128

Amino Acids

13.6

Weight (kDa)

4.73

Isoelectric Point (pI)

38.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CP12 PF02672 58 - 126 3.1e-27 CP12 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 281
AccIII TCCGGA 1 cut(s) 163
AciI CCGC 6 cut(s) 56, 187, 237, 251, 281, 284
AclWI GGATC 1 cut(s) 136
AcoI YGGCCR 1 cut(s) 231
AfaI GTAC 2 cut(s) 104, 370
AfiI CCNNNNNNNGG 2 cut(s) 151, 236
AgeI ACCGGT 1 cut(s) 148
AjnI CCWGG 2 cut(s) 37, 50
AleI CACNNNNGTG 1 cut(s) 297
AluBI AGCT 4 cut(s) 86, 96, 158, 274
AluI AGCT 4 cut(s) 86, 96, 158, 274
Alw21I GWGCWC 1 cut(s) 221
Alw26I GTCTC 2 cut(s) 56, 323
AlwI GGATC 1 cut(s) 136
Aor13HI TCCGGA 1 cut(s) 163
AoxI GGCC 1 cut(s) 231
ApeKI GCWGC 3 cut(s) 24, 155, 158
AsiGI ACCGGT 1 cut(s) 148
AspLEI GCGC 1 cut(s) 281
AspS9I GGNCC 2 cut(s) 226, 319
AsuC2I CCSGG 1 cut(s) 230
AsuHPI GGTGA 2 cut(s) 85, 103
AvaII GGWCC 2 cut(s) 226, 319
Bbv12I GWGCWC 1 cut(s) 221
BbvI GCAGC 3 cut(s) 36, 145, 167
BceAI ACGGC 1 cut(s) 351
BciT130I CCWGG 2 cut(s) 39, 52
BcnI CCSGG 1 cut(s) 230
BcoDI GTCTC 2 cut(s) 56, 323
BglI GCCNNNNNGGC 1 cut(s) 56
BisI GCNGC 7 cut(s) 25, 57, 156, 159, 252, 282, 285
BlpI GCTNAGC 2 cut(s) 207, 275
BlsI GCNGC 7 cut(s) 26, 58, 157, 160, 253, 283, 286
Bme1390I CCNGG 3 cut(s) 39, 52, 230
Bme18I GGWCC 2 cut(s) 226, 319
BmgT120I GGNCC 2 cut(s) 226, 319
BmiI GGNNCC 2 cut(s) 228, 321
BmrFI CCNGG 3 cut(s) 39, 52, 230
BmsI GCATC 1 cut(s) 204
BpmI CTGGAG 1 cut(s) 60
Bpu1102I GCTNAGC 2 cut(s) 207, 275
BpuMI CCSGG 1 cut(s) 230
BsaJI CCNNGG 4 cut(s) 37, 51, 69, 324
BsaWI WCCGGW 2 cut(s) 148, 163
Bsc4I CCNNNNNNNGG 2 cut(s) 151, 236
Bse118I RCCGGY 1 cut(s) 148
BseAI TCCGGA 1 cut(s) 163
BseBI CCWGG 2 cut(s) 39, 52
BseDI CCNNGG 4 cut(s) 37, 51, 69, 324
BseGI GGATG 3 cut(s) 138, 144, 265
BseLI CCNNNNNNNGG 2 cut(s) 151, 236
BseMII CTCAG 2 cut(s) 198, 266
BseRI GAGGAG 1 cut(s) 284
BseXI GCAGC 3 cut(s) 36, 145, 167
BseYI CCCAGC 1 cut(s) 86
Bsh1236I CGCG 1 cut(s) 281
Bsh1285I CGRYCG 1 cut(s) 149
BshFI GGCC 1 cut(s) 233
BshTI ACCGGT 1 cut(s) 148
BsiEI CGRYCG 1 cut(s) 149
BsiHKAI GWGCWC 1 cut(s) 221
BsiSI CCGG 3 cut(s) 149, 164, 230
BslI CCNNNNNNNGG 2 cut(s) 151, 236
BsmAI GTCTC 2 cut(s) 56, 323
BsnI GGCC 1 cut(s) 233
Bsp1286I GDGCHC 1 cut(s) 221
Bsp13I TCCGGA 1 cut(s) 163
Bsp143I GATC 1 cut(s) 141
Bsp1720I GCTNAGC 2 cut(s) 207, 275
BspACI CCGC 6 cut(s) 56, 187, 237, 251, 281, 284
BspANI GGCC 1 cut(s) 233
BspCNI CTCAG 2 cut(s) 199, 267
BspEI TCCGGA 1 cut(s) 163
BspFNI CGCG 1 cut(s) 281
BspLI GGNNCC 2 cut(s) 228, 321
BspPI GGATC 1 cut(s) 136
BsrFI RCCGGY 1 cut(s) 148
BssAI RCCGGY 1 cut(s) 148
BssECI CCNNGG 4 cut(s) 37, 51, 69, 324
BssMI GATC 1 cut(s) 141
BssT1I CCWWGG 2 cut(s) 69, 324
Bst2UI CCWGG 2 cut(s) 39, 52
BstC8I GCNNGC 5 cut(s) 84, 213, 217, 235, 289
BstDEI CTNAG 2 cut(s) 207, 275
BstF5I GGATG 3 cut(s) 138, 144, 265
BstFNI CGCG 1 cut(s) 281
BstHHI GCGC 1 cut(s) 281
BstKTI GATC 1 cut(s) 144
BstMAI GTCTC 2 cut(s) 56, 323
BstMBI GATC 1 cut(s) 141
BstMCI CGRYCG 1 cut(s) 149
BstMWI GCNNNNNNNGC 4 cut(s) 11, 56, 212, 243
BstNI CCWGG 2 cut(s) 39, 52
BstSCI CCNGG 3 cut(s) 37, 50, 228
BstUI CGCG 1 cut(s) 281
BstV1I GCAGC 3 cut(s) 36, 145, 167
BsuRI GGCC 1 cut(s) 233
BtsCI GGATG 3 cut(s) 138, 144, 265
Cac8I GCNNGC 5 cut(s) 84, 213, 217, 235, 289
CfoI GCGC 1 cut(s) 281
Cfr10I RCCGGY 1 cut(s) 148
Cfr13I GGNCC 2 cut(s) 226, 319
Csp6I GTAC 2 cut(s) 103, 369
CspAI ACCGGT 1 cut(s) 148
CviJI RGCY 9 cut(s) 27, 82, 86, 96, 158, 206, 233, 274, 291
CviKI_1 RGCY 9 cut(s) 27, 82, 86, 96, 158, 206, 233, 274, 291
CviQI GTAC 2 cut(s) 103, 369
DdeI CTNAG 2 cut(s) 207, 275
DpnI GATC 1 cut(s) 143
DpnII GATC 1 cut(s) 141
EaeI YGGCCR 1 cut(s) 231
Eco130I CCWWGG 2 cut(s) 69, 324
Eco47I GGWCC 2 cut(s) 226, 319
EcoO109I RGGNCCY 2 cut(s) 226, 319
EcoRII CCWGG 2 cut(s) 37, 50
EcoT14I CCWWGG 2 cut(s) 69, 324
ErhI CCWWGG 2 cut(s) 69, 324
FalI AAGNNNNNCTT 2 cut(s) 308, 340
FauI CCCGC 2 cut(s) 180, 230
Fnu4HI GCNGC 7 cut(s) 25, 57, 156, 159, 252, 282, 285
FokI GGATG 3 cut(s) 145, 151, 272
Fsp4HI GCNGC 7 cut(s) 25, 57, 156, 159, 252, 282, 285
GlaI GCGC 1 cut(s) 280
GluI GCNGC 7 cut(s) 25, 57, 156, 159, 252, 282, 285
GsaI CCCAGC 1 cut(s) 90
GsuI CTGGAG 1 cut(s) 60
HaeIII GGCC 1 cut(s) 233
HapII CCGG 3 cut(s) 149, 164, 230
HhaI GCGC 1 cut(s) 281
Hin6I GCGC 1 cut(s) 279
HinP1I GCGC 1 cut(s) 279
HinfI GANTC 1 cut(s) 42
HpaII CCGG 3 cut(s) 149, 164, 230
HphI GGTGA 2 cut(s) 85, 103
Hpy188I TCNGA 4 cut(s) 132, 146, 178, 223
Hpy188III TCNNGA 2 cut(s) 31, 164
Hpy99I CGWCG 1 cut(s) 362
HpyCH4V TGCA 1 cut(s) 339
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 56, 212, 243
HpyF3I CTNAG 2 cut(s) 207, 275
HspAI GCGC 1 cut(s) 279
Kpn2I TCCGGA 1 cut(s) 163
Kzo9I GATC 1 cut(s) 141
LmnI GCTCC 2 cut(s) 166, 271
Lsp1109I GCAGC 3 cut(s) 36, 145, 167
LweI GCATC 1 cut(s) 204
MaeIII GTNAC 1 cut(s) 299
MalI GATC 1 cut(s) 143
MboI GATC 1 cut(s) 141
MboII GAAGA 1 cut(s) 322
MhlI GDGCHC 1 cut(s) 221
MlyI GAGTC 1 cut(s) 51
MmeI TCCRAC 2 cut(s) 110, 169
MnlI CCTC 6 cut(s) 38, 129, 196, 217, 256, 262
MroI TCCGGA 1 cut(s) 163
MslI CAYNNNNRTG 1 cut(s) 297
MspA1I CMGCKG 3 cut(s) 86, 158, 237
MspI CCGG 3 cut(s) 149, 164, 230
MspR9I CCNGG 3 cut(s) 39, 52, 230
MvaI CCWGG 2 cut(s) 39, 52
MvnI CGCG 1 cut(s) 281
MwoI GCNNNNNNNGC 4 cut(s) 11, 56, 212, 243
NciI CCSGG 1 cut(s) 230
NdeII GATC 1 cut(s) 141
NlaIV GGNNCC 2 cut(s) 228, 321
NmuCI GTSAC 1 cut(s) 299
OliI CACNNNNGTG 1 cut(s) 297
PinAI ACCGGT 1 cut(s) 148
PkrI GCNGC 7 cut(s) 26, 58, 157, 160, 253, 283, 286
PleI GAGTC 1 cut(s) 50
PpsI GAGTC 1 cut(s) 50
PpuMI RGGWCCY 2 cut(s) 226, 319
Psp5II RGGWCCY 2 cut(s) 226, 319
Psp6I CCWGG 2 cut(s) 37, 50
PspFI CCCAGC 1 cut(s) 86
PspGI CCWGG 2 cut(s) 37, 50
PspN4I GGNNCC 2 cut(s) 228, 321
PspPI GGNCC 2 cut(s) 226, 319
PspPPI RGGWCCY 2 cut(s) 226, 319
PvuII CAGCTG 2 cut(s) 86, 158
RsaI GTAC 2 cut(s) 104, 370
RsaNI GTAC 2 cut(s) 103, 369
RseI CAYNNNNRTG 1 cut(s) 297
SatI GCNGC 7 cut(s) 25, 57, 156, 159, 252, 282, 285
Sau3AI GATC 1 cut(s) 141
Sau96I GGNCC 2 cut(s) 226, 319
SchI GAGTC 1 cut(s) 51
ScrFI CCNGG 3 cut(s) 39, 52, 230
SduI GDGCHC 1 cut(s) 221
SetI ASST 7 cut(s) 75, 88, 98, 160, 186, 267, 276
SfaNI GCATC 1 cut(s) 204
SinI GGWCC 2 cut(s) 226, 319
SmiMI CAYNNNNRTG 1 cut(s) 297
SsiI CCGC 6 cut(s) 56, 187, 237, 251, 281, 284
StyD4I CCNGG 3 cut(s) 37, 50, 228
StyI CCWWGG 2 cut(s) 69, 324
TaqI TCGA 1 cut(s) 32
TauI GCSGC 4 cut(s) 59, 254, 284, 287
TseFI GTSAC 1 cut(s) 299
TseI GCWGC 3 cut(s) 24, 155, 158
Tsp45I GTSAC 1 cut(s) 299
VpaK11BI GGWCC 2 cut(s) 226, 319
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.