RchiOBHm_Chr7g0241451

CP12

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0009967
Physical Location & Seq
Forward (+)
67540308 .. 67541000
693 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ21636

Sequence Viewer

Length: 483 bp
ATGCCAGCACCAATTAAACCCTCATGCTGGGCTCCTTATCACAACGCCATTCTATCACCCCACTTTTTTCAAAGCAGAAAACTTGTTAGAAGGAAAGAAATGGCAACAAGTGTAGCAGGAGTGAGCCTCTCGACCCCAAGAGTCTTTGCCAGGGCGGCAGAGACACCCAAGGTTATTAAGCCAGCTGGCCTGAGCTTCGGCTACTCGGCTGGGCGGGTGGGTTCGGGGCGGATGAGCATGATCCGACCGGTGGCAGCTGCCCCGGATAAACTATCGGAGAAGGTGCAGGAGAGCATCAAGGAGGCGGAGCAGGCGTGCTCGGAGGACCCGGCGAGCGGGGAGTGCGTGGCGGCGTGGGACGAGGTTGAGGAGCTGAGCGCGGCGGCCAGCCACAAGCGCGACAGCGAAAAGAACAAGGACCCCTTGGAGACTTACTGCAGCGACAACCCCGAAACCGACGAGTGCCGTACTTACGACAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CP12

Protein Analysis

160

Amino Acids

17.43

Weight (kDa)

5.96

Isoelectric Point (pI)

61.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CP12 PF02672 91 - 159 1.8e-27 CP12 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 336
AccII CGCG 2 cut(s) 380, 399
AciI CCGC 8 cut(s) 155, 214, 229, 305, 336, 350, 380, 383
AclWI GGATC 1 cut(s) 235
AcoI YGGCCR 1 cut(s) 384
AfaI GTAC 1 cut(s) 469
AfiI CCNNNNNNNGG 3 cut(s) 27, 250, 335
AgeI ACCGGT 1 cut(s) 247
AgsI TTSAA 1 cut(s) 71
AjnI CCWGG 1 cut(s) 149
AluBI AGCT 4 cut(s) 185, 195, 257, 373
AluI AGCT 4 cut(s) 185, 195, 257, 373
Alw21I GWGCWC 1 cut(s) 320
Alw26I GTCTC 2 cut(s) 155, 422
AlwI GGATC 1 cut(s) 235
AoxI GGCC 2 cut(s) 187, 384
ApeKI GCWGC 3 cut(s) 254, 257, 438
AsiGI ACCGGT 1 cut(s) 247
AspLEI GCGC 2 cut(s) 380, 399
AspS9I GGNCC 2 cut(s) 325, 418
AsuC2I CCSGG 2 cut(s) 263, 329
AsuHPI GGTGA 1 cut(s) 48
AvaII GGWCC 2 cut(s) 325, 418
BanII GRGCYC 1 cut(s) 34
Bbv12I GWGCWC 1 cut(s) 320
BbvI GCAGC 3 cut(s) 244, 266, 450
BceAI ACGGC 1 cut(s) 450
BciT130I CCWGG 1 cut(s) 151
BcnI CCSGG 2 cut(s) 263, 329
BcoDI GTCTC 2 cut(s) 155, 422
BfmI CTRYAG 1 cut(s) 436
BglI GCCNNNNNGGC 1 cut(s) 155
BisI GCNGC 7 cut(s) 156, 255, 258, 351, 381, 384, 439
BlpI GCTNAGC 1 cut(s) 374
BlsI GCNGC 7 cut(s) 157, 256, 259, 352, 382, 385, 440
Bme1390I CCNGG 3 cut(s) 151, 263, 329
Bme18I GGWCC 2 cut(s) 325, 418
BmgT120I GGNCC 2 cut(s) 325, 418
BmiI GGNNCC 3 cut(s) 33, 327, 420
BmrFI CCNGG 3 cut(s) 151, 263, 329
BmsI GCATC 1 cut(s) 303
BplI GAGNNNNNCTC 2 cut(s) 111, 143
Bpu10I CCTNAGC 1 cut(s) 191
Bpu1102I GCTNAGC 1 cut(s) 374
BpuMI CCSGG 2 cut(s) 263, 329
BsaJI CCNNGG 4 cut(s) 150, 168, 261, 423
BsaWI WCCGGW 1 cut(s) 247
BsaXI ACNNNNNCTCC 2 cut(s) 299, 329
Bsc4I CCNNNNNNNGG 3 cut(s) 27, 250, 335
Bse118I RCCGGY 1 cut(s) 247
BseBI CCWGG 1 cut(s) 151
BseDI CCNNGG 4 cut(s) 150, 168, 261, 423
BseGI GGATG 1 cut(s) 237
BseLI CCNNNNNNNGG 3 cut(s) 27, 250, 335
BseMII CTCAG 2 cut(s) 182, 365
BseRI GAGGAG 1 cut(s) 383
BseXI GCAGC 3 cut(s) 244, 266, 450
BseYI CCCAGC 2 cut(s) 27, 209
BsgI GTGCAG 1 cut(s) 305
Bsh1236I CGCG 2 cut(s) 380, 399
Bsh1285I CGRYCG 1 cut(s) 248
BshFI GGCC 2 cut(s) 189, 386
BshTI ACCGGT 1 cut(s) 247
BsiEI CGRYCG 1 cut(s) 248
BsiHKAI GWGCWC 1 cut(s) 320
BsiSI CCGG 3 cut(s) 248, 263, 329
BslFI GGGAC 1 cut(s) 371
BslI CCNNNNNNNGG 3 cut(s) 27, 250, 335
BsmAI GTCTC 2 cut(s) 155, 422
BsmFI GGGAC 1 cut(s) 371
BsnI GGCC 2 cut(s) 189, 386
Bsp1286I GDGCHC 2 cut(s) 34, 320
Bsp143I GATC 1 cut(s) 240
Bsp1720I GCTNAGC 1 cut(s) 374
BspACI CCGC 8 cut(s) 155, 214, 229, 305, 336, 350, 380, 383
BspANI GGCC 2 cut(s) 189, 386
BspCNI CTCAG 2 cut(s) 183, 366
BspFNI CGCG 2 cut(s) 380, 399
BspLI GGNNCC 3 cut(s) 33, 327, 420
BspMAI CTGCAG 1 cut(s) 440
BspPI GGATC 1 cut(s) 235
BsrBI CCGCTC 1 cut(s) 336
BsrFI RCCGGY 1 cut(s) 247
BssAI RCCGGY 1 cut(s) 247
BssECI CCNNGG 4 cut(s) 150, 168, 261, 423
BssMI GATC 1 cut(s) 240
BssT1I CCWWGG 2 cut(s) 168, 423
Bst2UI CCWGG 1 cut(s) 151
BstC8I GCNNGC 7 cut(s) 6, 183, 187, 312, 316, 334, 388
BstDEI CTNAG 2 cut(s) 191, 374
BstF5I GGATG 1 cut(s) 237
BstFNI CGCG 2 cut(s) 380, 399
BstHHI GCGC 2 cut(s) 380, 399
BstKTI GATC 1 cut(s) 243
BstMAI GTCTC 2 cut(s) 155, 422
BstMBI GATC 1 cut(s) 240
BstMCI CGRYCG 1 cut(s) 248
BstMWI GCNNNNNNNGC 4 cut(s) 155, 311, 342, 396
BstNI CCWGG 1 cut(s) 151
BstSCI CCNGG 3 cut(s) 149, 261, 327
BstSFI CTRYAG 1 cut(s) 436
BstUI CGCG 2 cut(s) 380, 399
BstV1I GCAGC 3 cut(s) 244, 266, 450
BsuRI GGCC 2 cut(s) 189, 386
BtsCI GGATG 1 cut(s) 237
Cac8I GCNNGC 7 cut(s) 6, 183, 187, 312, 316, 334, 388
CfoI GCGC 2 cut(s) 380, 399
Cfr10I RCCGGY 1 cut(s) 247
Cfr13I GGNCC 2 cut(s) 325, 418
Csp6I GTAC 1 cut(s) 468
CspAI ACCGGT 1 cut(s) 247
CviAII CATG 2 cut(s) 24, 238
CviQI GTAC 1 cut(s) 468
DdeI CTNAG 2 cut(s) 191, 374
DpnI GATC 1 cut(s) 242
DpnII GATC 1 cut(s) 240
EaeI YGGCCR 1 cut(s) 384
EciI GGCGGA 2 cut(s) 244, 320
Eco130I CCWWGG 2 cut(s) 168, 423
Eco24I GRGCYC 1 cut(s) 34
Eco47I GGWCC 2 cut(s) 325, 418
EcoO109I RGGNCCY 2 cut(s) 325, 418
EcoRII CCWGG 1 cut(s) 149
EcoT14I CCWWGG 2 cut(s) 168, 423
EcoT38I GRGCYC 1 cut(s) 34
ErhI CCWWGG 2 cut(s) 168, 423
FaeI CATG 2 cut(s) 27, 241
FaiI YATR 2 cut(s) 25, 239
FalI AAGNNNNNCTT 2 cut(s) 407, 439
FaqI GGGAC 1 cut(s) 371
FatI CATG 2 cut(s) 23, 237
FauI CCCGC 2 cut(s) 207, 329
Fnu4HI GCNGC 7 cut(s) 156, 255, 258, 351, 381, 384, 439
FokI GGATG 1 cut(s) 244
FriOI GRGCYC 1 cut(s) 34
Fsp4HI GCNGC 7 cut(s) 156, 255, 258, 351, 381, 384, 439
GlaI GCGC 2 cut(s) 379, 398
GluI GCNGC 7 cut(s) 156, 255, 258, 351, 381, 384, 439
GsaI CCCAGC 2 cut(s) 31, 213
HaeIII GGCC 2 cut(s) 189, 386
HapII CCGG 3 cut(s) 248, 263, 329
HhaI GCGC 2 cut(s) 380, 399
Hin1II CATG 2 cut(s) 27, 241
Hin6I GCGC 2 cut(s) 378, 397
HinP1I GCGC 2 cut(s) 378, 397
HinfI GANTC 1 cut(s) 141
HpaII CCGG 3 cut(s) 248, 263, 329
HphI GGTGA 1 cut(s) 48
Hpy188I TCNGA 3 cut(s) 245, 277, 322
Hpy188III TCNNGA 1 cut(s) 130
Hpy99I CGWCG 1 cut(s) 461
HpyAV CCTTC 2 cut(s) 84, 274
HpyCH4V TGCA 2 cut(s) 286, 438
HpyF10VI GCNNNNNNNGC 4 cut(s) 155, 311, 342, 396
HpyF3I CTNAG 2 cut(s) 191, 374
Hsp92II CATG 2 cut(s) 27, 241
HspAI GCGC 2 cut(s) 378, 397
Kzo9I GATC 1 cut(s) 240
LmnI GCTCC 3 cut(s) 37, 307, 370
Lsp1109I GCAGC 3 cut(s) 244, 266, 450
LweI GCATC 1 cut(s) 303
MalI GATC 1 cut(s) 242
MbiI CCGCTC 1 cut(s) 336
MboI GATC 1 cut(s) 240
MhlI GDGCHC 2 cut(s) 34, 320
MluCI AATT 1 cut(s) 12
MlyI GAGTC 1 cut(s) 150
MmeI TCCRAC 1 cut(s) 268
MnlI CCTC 6 cut(s) 31, 137, 295, 316, 355, 361
MseI TTAA 2 cut(s) 15, 177
MspA1I CMGCKG 2 cut(s) 185, 257
MspI CCGG 3 cut(s) 248, 263, 329
MspR9I CCNGG 3 cut(s) 151, 263, 329
MvaI CCWGG 1 cut(s) 151
MvnI CGCG 2 cut(s) 380, 399
MwoI GCNNNNNNNGC 4 cut(s) 155, 311, 342, 396
NciI CCSGG 2 cut(s) 263, 329
NdeII GATC 1 cut(s) 240
NlaIII CATG 2 cut(s) 27, 241
NlaIV GGNNCC 3 cut(s) 33, 327, 420
NmeAIII GCCGAG 1 cut(s) 185
PinAI ACCGGT 1 cut(s) 247
PkrI GCNGC 7 cut(s) 157, 256, 259, 352, 382, 385, 440
PleI GAGTC 1 cut(s) 149
PpsI GAGTC 1 cut(s) 149
PpuMI RGGWCCY 2 cut(s) 325, 418
Psp5II RGGWCCY 2 cut(s) 325, 418
Psp6I CCWGG 1 cut(s) 149
PspFI CCCAGC 2 cut(s) 27, 209
PspGI CCWGG 1 cut(s) 149
PspN4I GGNNCC 3 cut(s) 33, 327, 420
PspPI GGNCC 2 cut(s) 325, 418
PspPPI RGGWCCY 2 cut(s) 325, 418
PstI CTGCAG 1 cut(s) 440
PvuII CAGCTG 2 cut(s) 185, 257
RsaI GTAC 1 cut(s) 469
RsaNI GTAC 1 cut(s) 468
SaqAI TTAA 2 cut(s) 15, 177
SatI GCNGC 7 cut(s) 156, 255, 258, 351, 381, 384, 439
Sau3AI GATC 1 cut(s) 240
Sau96I GGNCC 2 cut(s) 325, 418
SchI GAGTC 1 cut(s) 150
ScrFI CCNGG 3 cut(s) 151, 263, 329
SduI GDGCHC 2 cut(s) 34, 320
SetI ASST 7 cut(s) 174, 187, 197, 259, 285, 366, 375
SfaNI GCATC 1 cut(s) 303
SfcI CTRYAG 1 cut(s) 436
SinI GGWCC 2 cut(s) 325, 418
Sse9I AATT 1 cut(s) 12
SsiI CCGC 8 cut(s) 155, 214, 229, 305, 336, 350, 380, 383
StyD4I CCNGG 3 cut(s) 149, 261, 327
StyI CCWWGG 2 cut(s) 168, 423
TaqI TCGA 1 cut(s) 131
TasI AATT 1 cut(s) 12
TauI GCSGC 4 cut(s) 158, 353, 383, 386
Tru1I TTAA 2 cut(s) 15, 177
Tru9I TTAA 2 cut(s) 15, 177
TseI GCWGC 3 cut(s) 254, 257, 438
VpaK11BI GGWCC 2 cut(s) 325, 418
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.