MD14G1018200.v1.1

Calvin cycle protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0009967
Physical Location & Seq
Forward (+)
1710794 .. 1711183
390 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1018200.v1.1.491

Sequence Viewer

Length: 390 bp
ATGGCAACCACCACTACACTCGCAGAAGTGAGCTTCTCCACCCCAAGGGCGGTGTTGTCAAAAGCAACAGAGGCACTGATGACATTTCAGTCACTCAACATCCCCACCTGGAGAAGCTACTCTTCCATTAGCCGCTGCATGACCTTGGTAAGGCCAGTAAGCGCAGCACCGGTTAAACTGTCGGAAAAGGTGGCGGAAAGCATCAAGATCGTCGAAGAGAAGTGCTCAGACGACCCGGAAAACGGGGAGTGCGTAGTGGCGTGGGACAAGGTGGAGGAGCTGAGCGCTGCCGCCAGCCACAAACGTGCCAACGAGGAAAGTTCCGACCCCTTAGAGACATTATGTAAGGATAACCCTGAAACCGACGAGTGTCGTACTTACAACAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CP12

Protein Analysis

130

Amino Acids

14.19

Weight (kDa)

4.75

Isoelectric Point (pI)

52.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CP12 PF02672 60 - 128 4.8e-21 CP12 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 88
AciI CCGC 4 cut(s) 50, 133, 194, 291
AfaI GTAC 1 cut(s) 376
AfeI AGCGCT 1 cut(s) 286
AfiI CCNNNNNNNGG 4 cut(s) 45, 49, 150, 242
AgeI ACCGGT 1 cut(s) 169
AjnI CCWGG 1 cut(s) 107
AleI CACNNNNGTG 1 cut(s) 303
AluBI AGCT 3 cut(s) 33, 117, 280
AluI AGCT 3 cut(s) 33, 117, 280
Alw21I GWGCWC 1 cut(s) 227
Alw26I GTCTC 1 cut(s) 329
Aor51HI AGCGCT 1 cut(s) 286
AoxI GGCC 1 cut(s) 152
ApeKI GCWGC 3 cut(s) 135, 164, 287
AsiGI ACCGGT 1 cut(s) 169
AspLEI GCGC 2 cut(s) 164, 287
AsuC2I CCSGG 1 cut(s) 236
Bbv12I GWGCWC 1 cut(s) 227
BbvI GCAGC 3 cut(s) 122, 176, 274
BcgI CGANNNNNNTGC 2 cut(s) 190, 224
BciT130I CCWGG 1 cut(s) 109
BcnI CCSGG 1 cut(s) 236
BcoDI GTCTC 1 cut(s) 329
BfoI RGCGCY 1 cut(s) 288
BisI GCNGC 5 cut(s) 133, 136, 165, 288, 291
BlpI GCTNAGC 1 cut(s) 281
BlsI GCNGC 5 cut(s) 134, 137, 166, 289, 292
Bme1390I CCNGG 2 cut(s) 109, 236
BmrFI CCNGG 2 cut(s) 109, 236
BmsI GCATC 1 cut(s) 210
BoxI GACNNNNGTC 1 cut(s) 369
BplI GAGNNNNNCTC 2 cut(s) 209, 241
BpmI CTGGAG 1 cut(s) 130
Bpu1102I GCTNAGC 1 cut(s) 281
BpuMI CCSGG 1 cut(s) 236
BsaJI CCNNGG 2 cut(s) 44, 144
BsaWI WCCGGW 1 cut(s) 169
Bsc4I CCNNNNNNNGG 4 cut(s) 45, 49, 150, 242
Bse118I RCCGGY 1 cut(s) 169
Bse1I ACTGG 1 cut(s) 155
BseBI CCWGG 1 cut(s) 109
BseDI CCNNGG 2 cut(s) 44, 144
BseGI GGATG 1 cut(s) 99
BseLI CCNNNNNNNGG 4 cut(s) 45, 49, 150, 242
BseMII CTCAG 2 cut(s) 240, 272
BseNI ACTGG 1 cut(s) 155
BseRI GAGGAG 1 cut(s) 290
BseXI GCAGC 3 cut(s) 122, 176, 274
BshFI GGCC 1 cut(s) 154
BshTI ACCGGT 1 cut(s) 169
BsiHKAI GWGCWC 1 cut(s) 227
BsiSI CCGG 2 cut(s) 170, 236
BslFI GGGAC 1 cut(s) 278
BslI CCNNNNNNNGG 4 cut(s) 45, 49, 150, 242
BsmAI GTCTC 1 cut(s) 329
BsmFI GGGAC 1 cut(s) 278
BsnI GGCC 1 cut(s) 154
Bsp1286I GDGCHC 1 cut(s) 227
Bsp143I GATC 1 cut(s) 207
Bsp1720I GCTNAGC 1 cut(s) 281
BspACI CCGC 4 cut(s) 50, 133, 194, 291
BspANI GGCC 1 cut(s) 154
BspCNI CTCAG 2 cut(s) 239, 273
BsrFI RCCGGY 1 cut(s) 169
BsrI ACTGG 1 cut(s) 155
BssAI RCCGGY 1 cut(s) 169
BssECI CCNNGG 2 cut(s) 44, 144
BssMI GATC 1 cut(s) 207
BssT1I CCWWGG 2 cut(s) 44, 144
Bst2UI CCWGG 1 cut(s) 109
Bst4CI ACNGT 1 cut(s) 180
Bst6I CTCTTC 2 cut(s) 127, 210
BstC8I GCNNGC 1 cut(s) 295
BstDEI CTNAG 3 cut(s) 226, 281, 331
BstENI CCTNNNNNAGG 1 cut(s) 148
BstF5I GGATG 1 cut(s) 99
BstH2I RGCGCY 1 cut(s) 288
BstHHI GCGC 2 cut(s) 164, 287
BstKTI GATC 1 cut(s) 210
BstMAI GTCTC 1 cut(s) 329
BstMBI GATC 1 cut(s) 207
BstMWI GCNNNNNNNGC 1 cut(s) 71
BstNI CCWGG 1 cut(s) 109
BstPAI GACNNNNGTC 1 cut(s) 369
BstSCI CCNGG 2 cut(s) 107, 234
BstV1I GCAGC 3 cut(s) 122, 176, 274
BsuRI GGCC 1 cut(s) 154
BtsCI GGATG 1 cut(s) 99
BtsIMutI CAGTG 1 cut(s) 74
Cac8I GCNNGC 1 cut(s) 295
CfoI GCGC 2 cut(s) 164, 287
Cfr10I RCCGGY 1 cut(s) 169
Csp6I GTAC 1 cut(s) 375
CspAI ACCGGT 1 cut(s) 169
CviAII CATG 1 cut(s) 139
CviJI RGCY 6 cut(s) 33, 117, 132, 154, 280, 297
CviKI_1 RGCY 6 cut(s) 33, 117, 132, 154, 280, 297
CviQI GTAC 1 cut(s) 375
DdeI CTNAG 3 cut(s) 226, 281, 331
DpnI GATC 1 cut(s) 209
DpnII GATC 1 cut(s) 207
DrdI GACNNNNNNGTC 1 cut(s) 88
DseDI GACNNNNNNGTC 1 cut(s) 88
Eam1104I CTCTTC 2 cut(s) 127, 210
EarI CTCTTC 2 cut(s) 127, 210
EciI GGCGGA 1 cut(s) 209
Eco130I CCWWGG 2 cut(s) 44, 144
Eco47III AGCGCT 1 cut(s) 286
EcoNI CCTNNNNNAGG 1 cut(s) 148
EcoRII CCWGG 1 cut(s) 107
EcoT14I CCWWGG 2 cut(s) 44, 144
ErhI CCWWGG 2 cut(s) 44, 144
FaeI CATG 1 cut(s) 142
FaiI YATR 2 cut(s) 140, 343
FalI AAGNNNNNCTT 2 cut(s) 106, 138
FaqI GGGAC 1 cut(s) 278
FatI CATG 1 cut(s) 138
Fnu4HI GCNGC 5 cut(s) 133, 136, 165, 288, 291
FokI GGATG 1 cut(s) 86
Fsp4HI GCNGC 5 cut(s) 133, 136, 165, 288, 291
GlaI GCGC 2 cut(s) 163, 286
GluI GCNGC 5 cut(s) 133, 136, 165, 288, 291
GsuI CTGGAG 1 cut(s) 130
HaeII RGCGCY 1 cut(s) 288
HaeIII GGCC 1 cut(s) 154
HapII CCGG 2 cut(s) 170, 236
HhaI GCGC 2 cut(s) 164, 287
Hin1II CATG 1 cut(s) 142
Hin6I GCGC 2 cut(s) 162, 285
HinP1I GCGC 2 cut(s) 162, 285
HpaII CCGG 2 cut(s) 170, 236
Hpy188I TCNGA 3 cut(s) 184, 229, 325
Hpy188III TCNNGA 1 cut(s) 205
Hpy99I CGWCG 2 cut(s) 215, 368
HpyCH4III ACNGT 1 cut(s) 180
HpyCH4IV ACGT 1 cut(s) 304
HpyCH4V TGCA 1 cut(s) 138
HpyF10VI GCNNNNNNNGC 1 cut(s) 71
HpyF3I CTNAG 3 cut(s) 226, 281, 331
HpySE526I ACGT 1 cut(s) 304
Hsp92II CATG 1 cut(s) 142
HspAI GCGC 2 cut(s) 162, 285
Kzo9I GATC 1 cut(s) 207
LmnI GCTCC 1 cut(s) 277
LpnPI CCDG 7 cut(s) 94, 121, 168, 183, 249, 307, 369
Lsp1109I GCAGC 3 cut(s) 122, 176, 274
LweI GCATC 1 cut(s) 210
MaeII ACGT 1 cut(s) 304
MaeIII GTNAC 1 cut(s) 90
MalI GATC 1 cut(s) 209
MboI GATC 1 cut(s) 207
MboII GAAGA 2 cut(s) 114, 227
MhlI GDGCHC 1 cut(s) 227
MmeI TCCRAC 2 cut(s) 162, 348
MnlI CCTC 3 cut(s) 64, 268, 307
MseI TTAA 1 cut(s) 174
MslI CAYNNNNRTG 1 cut(s) 303
MspA1I CMGCKG 1 cut(s) 135
MspI CCGG 2 cut(s) 170, 236
MspR9I CCNGG 2 cut(s) 109, 236
MvaI CCWGG 1 cut(s) 109
MwoI GCNNNNNNNGC 1 cut(s) 71
NciI CCSGG 1 cut(s) 236
NdeII GATC 1 cut(s) 207
NlaIII CATG 1 cut(s) 142
NmuCI GTSAC 1 cut(s) 90
OliI CACNNNNGTG 1 cut(s) 303
PcsI WCGNNNNNNNCGW 1 cut(s) 249
PinAI ACCGGT 1 cut(s) 169
PkrI GCNGC 5 cut(s) 134, 137, 166, 289, 292
PshAI GACNNNNGTC 1 cut(s) 369
Psp6I CCWGG 1 cut(s) 107
PspGI CCWGG 1 cut(s) 107
RsaI GTAC 1 cut(s) 376
RsaNI GTAC 1 cut(s) 375
RseI CAYNNNNRTG 1 cut(s) 303
SaqAI TTAA 1 cut(s) 174
SatI GCNGC 5 cut(s) 133, 136, 165, 288, 291
Sau3AI GATC 1 cut(s) 207
ScrFI CCNGG 2 cut(s) 109, 236
SduI GDGCHC 1 cut(s) 227
SetI ASST 8 cut(s) 35, 110, 119, 146, 192, 273, 282, 307
SfaNI GCATC 1 cut(s) 210
SmiMI CAYNNNNRTG 1 cut(s) 303
SsiI CCGC 4 cut(s) 50, 133, 194, 291
StyD4I CCNGG 2 cut(s) 107, 234
StyI CCWWGG 2 cut(s) 44, 144
TaaI ACNGT 1 cut(s) 180
TaiI ACGT 1 cut(s) 307
TaqI TCGA 1 cut(s) 213
TauI GCSGC 2 cut(s) 135, 293
Tru1I TTAA 1 cut(s) 174
Tru9I TTAA 1 cut(s) 174
TscAI CASTG 1 cut(s) 81
TseFI GTSAC 1 cut(s) 90
TseI GCWGC 3 cut(s) 135, 164, 287
Tsp45I GTSAC 1 cut(s) 90
TspRI CASTG 1 cut(s) 81
XagI CCTNNNNNAGG 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.