pycom03g23330

Calvin cycle protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0009967
Physical Location & Seq
Forward (+)
23191487 .. 23191876
390 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g23330.1

Sequence Viewer

Length: 390 bp
ATGGCAACCACCACTACAGTCGCCGGAGTGAGCCTCTCCACCCCAAGGGCAGTGTTGTCAAAAGCAACAGAGGCACCGAGGGCATTTCAATCACTCAACATCCCCACCTGGAGAAGCTACTCTTCCGTTGGCCGCCGCATGACCATGGTAAGGCCAGTAAGCGCAGCACCAGATAAACTGTCGGAAAAGGTGGCGGAAAGCATCAAGAGCGCCGAGGAGAAGTGCTCGGACGACCCGGCAAGCGGGGAGTGCGTGGCGGCGTGGGACGAGGTGGAGGAGCTGAGCGCTGCCGCCAGCCACAAACGTGACAACCAGAAAAGTTCCGACCCCCTGGAGACATTCTGTAACGACAACCCCGAAACCGACGAGTGCCGTACTTACGACAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CP12

Protein Analysis

130

Amino Acids

13.96

Weight (kDa)

4.81

Isoelectric Point (pI)

53.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CP12 PF02672 60 - 128 2.1e-25 CP12 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 73
AciI CCGC 6 cut(s) 133, 136, 194, 243, 257, 291
AcoI YGGCCR 1 cut(s) 130
AfaI GTAC 1 cut(s) 376
AfeI AGCGCT 1 cut(s) 286
AfiI CCNNNNNNNGG 3 cut(s) 45, 150, 242
AgsI TTSAA 1 cut(s) 89
AjnI CCWGG 2 cut(s) 107, 330
AleI CACNNNNGTG 1 cut(s) 303
AluBI AGCT 3 cut(s) 117, 280, 387
AluI AGCT 3 cut(s) 117, 280, 387
Alw21I GWGCWC 1 cut(s) 227
Alw26I GTCTC 1 cut(s) 329
Aor51HI AGCGCT 1 cut(s) 286
AoxI GGCC 2 cut(s) 130, 152
ApeKI GCWGC 2 cut(s) 164, 287
AspLEI GCGC 3 cut(s) 164, 212, 287
AsuC2I CCSGG 1 cut(s) 236
BanI GGYRCC 1 cut(s) 73
Bbv12I GWGCWC 1 cut(s) 227
BbvI GCAGC 2 cut(s) 176, 274
BceAI ACGGC 1 cut(s) 357
BciT130I CCWGG 2 cut(s) 109, 332
BcnI CCSGG 1 cut(s) 236
BcoDI GTCTC 1 cut(s) 329
BfmI CTRYAG 1 cut(s) 15
BfoI RGCGCY 2 cut(s) 213, 288
BisI GCNGC 6 cut(s) 133, 136, 165, 258, 288, 291
BlpI GCTNAGC 1 cut(s) 281
BlsI GCNGC 6 cut(s) 134, 137, 166, 259, 289, 292
Bme1390I CCNGG 3 cut(s) 109, 236, 332
BmiI GGNNCC 1 cut(s) 75
BmrFI CCNGG 3 cut(s) 109, 236, 332
BmsI GCATC 1 cut(s) 210
BplI GAGNNNNNCTC 4 cut(s) 18, 50, 209, 241
BpmI CTGGAG 2 cut(s) 130, 353
Bpu1102I GCTNAGC 1 cut(s) 281
BpuMI CCSGG 1 cut(s) 236
BsaJI CCNNGG 5 cut(s) 44, 77, 144, 213, 330
Bsc4I CCNNNNNNNGG 3 cut(s) 45, 150, 242
Bse1I ACTGG 1 cut(s) 155
BseBI CCWGG 2 cut(s) 109, 332
BseDI CCNNGG 5 cut(s) 44, 77, 144, 213, 330
BseGI GGATG 1 cut(s) 99
BseLI CCNNNNNNNGG 3 cut(s) 45, 150, 242
BseMII CTCAG 1 cut(s) 272
BseNI ACTGG 1 cut(s) 155
BseRI GAGGAG 2 cut(s) 230, 290
BseXI GCAGC 2 cut(s) 176, 274
BshFI GGCC 2 cut(s) 132, 154
BshNI GGYRCC 1 cut(s) 73
BsiHKAI GWGCWC 1 cut(s) 227
BsiSI CCGG 2 cut(s) 24, 236
BslFI GGGAC 1 cut(s) 278
BslI CCNNNNNNNGG 3 cut(s) 45, 150, 242
BsmAI GTCTC 1 cut(s) 329
BsmFI GGGAC 1 cut(s) 278
BsnI GGCC 2 cut(s) 132, 154
Bsp1286I GDGCHC 1 cut(s) 227
Bsp1720I GCTNAGC 1 cut(s) 281
Bsp19I CCATGG 1 cut(s) 144
BspACI CCGC 6 cut(s) 133, 136, 194, 243, 257, 291
BspANI GGCC 2 cut(s) 132, 154
BspCNI CTCAG 1 cut(s) 273
BspLI GGNNCC 1 cut(s) 75
BspT107I GGYRCC 1 cut(s) 73
BsrI ACTGG 1 cut(s) 155
BssECI CCNNGG 5 cut(s) 44, 77, 144, 213, 330
BssT1I CCWWGG 2 cut(s) 44, 144
Bst2UI CCWGG 2 cut(s) 109, 332
Bst4CI ACNGT 2 cut(s) 19, 180
Bst6I CTCTTC 1 cut(s) 127
BstC8I GCNNGC 2 cut(s) 241, 295
BstDEI CTNAG 1 cut(s) 281
BstDSI CCRYGG 1 cut(s) 144
BstF5I GGATG 1 cut(s) 99
BstH2I RGCGCY 2 cut(s) 213, 288
BstHHI GCGC 3 cut(s) 164, 212, 287
BstMAI GTCTC 1 cut(s) 329
BstMWI GCNNNNNNNGC 4 cut(s) 71, 80, 207, 249
BstNI CCWGG 2 cut(s) 109, 332
BstSCI CCNGG 3 cut(s) 107, 234, 330
BstSFI CTRYAG 1 cut(s) 15
BstV1I GCAGC 2 cut(s) 176, 274
BsuRI GGCC 2 cut(s) 132, 154
BtgI CCRYGG 1 cut(s) 144
BtsCI GGATG 1 cut(s) 99
BtsI GCAGTG 1 cut(s) 57
BtsIMutI CAGTG 1 cut(s) 57
Cac8I GCNNGC 2 cut(s) 241, 295
CfoI GCGC 3 cut(s) 164, 212, 287
Csp6I GTAC 1 cut(s) 375
CviAII CATG 2 cut(s) 139, 145
CviJI RGCY 7 cut(s) 33, 117, 132, 154, 280, 297, 387
CviKI_1 RGCY 7 cut(s) 33, 117, 132, 154, 280, 297, 387
CviQI GTAC 1 cut(s) 375
DdeI CTNAG 1 cut(s) 281
EaeI YGGCCR 1 cut(s) 130
Eam1104I CTCTTC 1 cut(s) 127
EarI CTCTTC 1 cut(s) 127
EciI GGCGGA 1 cut(s) 209
Eco130I CCWWGG 2 cut(s) 44, 144
Eco47III AGCGCT 1 cut(s) 286
EcoRII CCWGG 2 cut(s) 107, 330
EcoT14I CCWWGG 2 cut(s) 44, 144
ErhI CCWWGG 2 cut(s) 44, 144
FaeI CATG 2 cut(s) 142, 148
FaiI YATR 2 cut(s) 140, 146
FalI AAGNNNNNCTT 2 cut(s) 106, 138
FaqI GGGAC 1 cut(s) 278
FatI CATG 2 cut(s) 138, 144
FauI CCCGC 1 cut(s) 236
Fnu4HI GCNGC 6 cut(s) 133, 136, 165, 258, 288, 291
FokI GGATG 1 cut(s) 86
Fsp4HI GCNGC 6 cut(s) 133, 136, 165, 258, 288, 291
GlaI GCGC 3 cut(s) 163, 211, 286
GluI GCNGC 6 cut(s) 133, 136, 165, 258, 288, 291
GsuI CTGGAG 2 cut(s) 130, 353
HaeII RGCGCY 2 cut(s) 213, 288
HaeIII GGCC 2 cut(s) 132, 154
HapII CCGG 2 cut(s) 24, 236
HhaI GCGC 3 cut(s) 164, 212, 287
Hin1II CATG 2 cut(s) 142, 148
Hin6I GCGC 3 cut(s) 162, 210, 285
HinP1I GCGC 3 cut(s) 162, 210, 285
HpaII CCGG 2 cut(s) 24, 236
Hpy188I TCNGA 3 cut(s) 184, 229, 325
Hpy188III TCNNGA 1 cut(s) 205
Hpy99I CGWCG 1 cut(s) 368
HpyCH4III ACNGT 2 cut(s) 19, 180
HpyCH4IV ACGT 1 cut(s) 304
HpyF10VI GCNNNNNNNGC 4 cut(s) 71, 80, 207, 249
HpyF3I CTNAG 1 cut(s) 281
HpySE526I ACGT 1 cut(s) 304
Hsp92II CATG 2 cut(s) 142, 148
HspAI GCGC 3 cut(s) 162, 210, 285
LmnI GCTCC 1 cut(s) 277
Lsp1109I GCAGC 2 cut(s) 176, 274
LweI GCATC 1 cut(s) 210
MaeII ACGT 1 cut(s) 304
MaeIII GTNAC 2 cut(s) 305, 344
MboII GAAGA 1 cut(s) 114
MhlI GDGCHC 1 cut(s) 227
MmeI TCCRAC 2 cut(s) 162, 348
MnlI CCTC 6 cut(s) 44, 64, 72, 208, 262, 268
MslI CAYNNNNRTG 2 cut(s) 143, 303
MspA1I CMGCKG 1 cut(s) 387
MspI CCGG 2 cut(s) 24, 236
MspR9I CCNGG 3 cut(s) 109, 236, 332
MvaI CCWGG 2 cut(s) 109, 332
MwoI GCNNNNNNNGC 4 cut(s) 71, 80, 207, 249
NciI CCSGG 1 cut(s) 236
NcoI CCATGG 1 cut(s) 144
NlaIII CATG 2 cut(s) 142, 148
NlaIV GGNNCC 1 cut(s) 75
NmeAIII GCCGAG 1 cut(s) 238
NmuCI GTSAC 1 cut(s) 305
OliI CACNNNNGTG 1 cut(s) 303
PcsI WCGNNNNNNNCGW 1 cut(s) 354
PkrI GCNGC 6 cut(s) 134, 137, 166, 259, 289, 292
Psp6I CCWGG 2 cut(s) 107, 330
PspGI CCWGG 2 cut(s) 107, 330
PspN4I GGNNCC 1 cut(s) 75
PvuII CAGCTG 1 cut(s) 387
RsaI GTAC 1 cut(s) 376
RsaNI GTAC 1 cut(s) 375
RseI CAYNNNNRTG 2 cut(s) 143, 303
SatI GCNGC 6 cut(s) 133, 136, 165, 258, 288, 291
ScrFI CCNGG 3 cut(s) 109, 236, 332
SduI GDGCHC 1 cut(s) 227
SetI ASST 7 cut(s) 110, 119, 192, 273, 282, 307, 389
SfaNI GCATC 1 cut(s) 210
SfcI CTRYAG 1 cut(s) 15
SmiMI CAYNNNNRTG 2 cut(s) 143, 303
SsiI CCGC 6 cut(s) 133, 136, 194, 243, 257, 291
StyD4I CCNGG 3 cut(s) 107, 234, 330
StyI CCWWGG 2 cut(s) 44, 144
TaaI ACNGT 2 cut(s) 19, 180
TaiI ACGT 1 cut(s) 307
TauI GCSGC 4 cut(s) 135, 138, 260, 293
TscAI CASTG 1 cut(s) 57
TseFI GTSAC 1 cut(s) 305
TseI GCWGC 2 cut(s) 164, 287
Tsp45I GTSAC 1 cut(s) 305
TspGWI ACGGA 1 cut(s) 115
TspRI CASTG 1 cut(s) 57
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.