MD11G1314000.v1.1

Calvin cycle protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0009967
Physical Location & Seq
Forward (+)
42647389 .. 42647778
390 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1314000.v1.1.491

Sequence Viewer

Length: 390 bp
ATGGCAACCAGCATCACAGTCGCCGGAGTGAGCCTCTCCACCCCAAGGGCAGTGTTGTCGAAAGCAACAGAGGTACCGAGGGCATTTCACTCACTAAACGTTCCCACCTGGAGAAGCTACTCCTCCGTTGGCCGCCGCATGACCATGGTAAGGCCAGTGAGCGCAGCACCAGATAAACTGTCCGAAAAGGTGGCGGAAAGCATCAAGAGCGCCGAGGAGACCTGCTCGGACGACCCGGCAAGCGGGGAGTGCGTAGCGGCATGGGACGAGGTGGAGGAGCTGAGCGCTGCCGTCAGCCACAAACGCGACAACGAAAAAAGTTCCGACCCCTTGGAGACTTTCTGTAAGGACAACCCCGAAACCGACGAGTGCCGTACTTACGACAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CP12

Protein Analysis

130

Amino Acids

14.03

Weight (kDa)

4.83

Isoelectric Point (pI)

47.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CP12 PF02672 60 - 128 7.9e-26 CP12 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 230
Acc65I GGTACC 1 cut(s) 73
AccB1I GGYRCC 1 cut(s) 73
AccII CGCG 1 cut(s) 306
AciI CCGC 5 cut(s) 133, 136, 194, 243, 257
AclI AACGTT 1 cut(s) 99
AcoI YGGCCR 1 cut(s) 130
AfaI GTAC 2 cut(s) 75, 376
AfeI AGCGCT 1 cut(s) 286
AfiI CCNNNNNNNGG 3 cut(s) 45, 150, 242
AjnI CCWGG 1 cut(s) 107
AluBI AGCT 2 cut(s) 117, 280
AluI AGCT 2 cut(s) 117, 280
Alw26I GTCTC 2 cut(s) 212, 329
Aor51HI AGCGCT 1 cut(s) 286
AoxI GGCC 2 cut(s) 130, 152
ApeKI GCWGC 2 cut(s) 164, 287
Asp718I GGTACC 1 cut(s) 73
AspLEI GCGC 3 cut(s) 164, 212, 287
AsuC2I CCSGG 1 cut(s) 236
BaeI ACNNNNGTAYC 2 cut(s) 57, 90
BanI GGYRCC 1 cut(s) 73
BbvI GCAGC 2 cut(s) 176, 274
BceAI ACGGC 2 cut(s) 275, 357
BcgI CGANNNNNNTGC 3 cut(s) 35, 39, 73
BciT130I CCWGG 1 cut(s) 109
BcnI CCSGG 1 cut(s) 236
BcoDI GTCTC 2 cut(s) 212, 329
BfoI RGCGCY 2 cut(s) 213, 288
BfuAI ACCTGC 1 cut(s) 230
BisI GCNGC 5 cut(s) 133, 136, 165, 258, 288
BlpI GCTNAGC 1 cut(s) 281
BlsI GCNGC 5 cut(s) 134, 137, 166, 259, 289
Bme1390I CCNGG 2 cut(s) 109, 236
BmiI GGNNCC 1 cut(s) 75
BmrFI CCNGG 2 cut(s) 109, 236
BmsI GCATC 2 cut(s) 21, 210
BplI GAGNNNNNCTC 4 cut(s) 18, 50, 209, 241
BpmI CTGGAG 1 cut(s) 130
Bpu1102I GCTNAGC 1 cut(s) 281
BpuMI CCSGG 1 cut(s) 236
BsaI GGTCTC 1 cut(s) 212
BsaJI CCNNGG 5 cut(s) 44, 77, 144, 213, 330
Bsc4I CCNNNNNNNGG 3 cut(s) 45, 150, 242
Bse1I ACTGG 1 cut(s) 155
BseBI CCWGG 1 cut(s) 109
BseDI CCNNGG 5 cut(s) 44, 77, 144, 213, 330
BseLI CCNNNNNNNGG 3 cut(s) 45, 150, 242
BseMII CTCAG 1 cut(s) 272
BseNI ACTGG 1 cut(s) 155
BseRI GAGGAG 3 cut(s) 112, 230, 290
BseXI GCAGC 2 cut(s) 176, 274
Bsh1236I CGCG 1 cut(s) 306
BshFI GGCC 2 cut(s) 132, 154
BshNI GGYRCC 1 cut(s) 73
BsiSI CCGG 2 cut(s) 24, 236
BslFI GGGAC 1 cut(s) 278
BslI CCNNNNNNNGG 3 cut(s) 45, 150, 242
BsmAI GTCTC 2 cut(s) 212, 329
BsmFI GGGAC 1 cut(s) 278
BsnI GGCC 2 cut(s) 132, 154
Bso31I GGTCTC 1 cut(s) 212
Bsp1720I GCTNAGC 1 cut(s) 281
Bsp19I CCATGG 1 cut(s) 144
BspACI CCGC 5 cut(s) 133, 136, 194, 243, 257
BspANI GGCC 2 cut(s) 132, 154
BspCNI CTCAG 1 cut(s) 273
BspFNI CGCG 1 cut(s) 306
BspLI GGNNCC 1 cut(s) 75
BspMI ACCTGC 1 cut(s) 230
BspT107I GGYRCC 1 cut(s) 73
BspTNI GGTCTC 1 cut(s) 212
BsrI ACTGG 1 cut(s) 155
BssECI CCNNGG 5 cut(s) 44, 77, 144, 213, 330
BssT1I CCWWGG 3 cut(s) 44, 144, 330
Bst2UI CCWGG 1 cut(s) 109
Bst4CI ACNGT 2 cut(s) 19, 180
BstC8I GCNNGC 1 cut(s) 241
BstDEI CTNAG 1 cut(s) 281
BstDSI CCRYGG 1 cut(s) 144
BstFNI CGCG 1 cut(s) 306
BstH2I RGCGCY 2 cut(s) 213, 288
BstHHI GCGC 3 cut(s) 164, 212, 287
BstMAI GTCTC 2 cut(s) 212, 329
BstMWI GCNNNNNNNGC 3 cut(s) 207, 249, 303
BstNI CCWGG 1 cut(s) 109
BstSCI CCNGG 2 cut(s) 107, 234
BstUI CGCG 1 cut(s) 306
BstV1I GCAGC 2 cut(s) 176, 274
BsuRI GGCC 2 cut(s) 132, 154
BtgI CCRYGG 1 cut(s) 144
BtsI GCAGTG 1 cut(s) 57
BtsIMutI CAGTG 2 cut(s) 57, 162
BveI ACCTGC 1 cut(s) 230
Cac8I GCNNGC 1 cut(s) 241
CfoI GCGC 3 cut(s) 164, 212, 287
Csp6I GTAC 2 cut(s) 74, 375
CviAII CATG 3 cut(s) 139, 145, 261
CviJI RGCY 6 cut(s) 33, 117, 132, 154, 280, 297
CviKI_1 RGCY 6 cut(s) 33, 117, 132, 154, 280, 297
CviQI GTAC 2 cut(s) 74, 375
DdeI CTNAG 1 cut(s) 281
EaeI YGGCCR 1 cut(s) 130
EciI GGCGGA 1 cut(s) 209
Eco130I CCWWGG 3 cut(s) 44, 144, 330
Eco31I GGTCTC 1 cut(s) 212
Eco47III AGCGCT 1 cut(s) 286
EcoRII CCWGG 1 cut(s) 107
EcoT14I CCWWGG 3 cut(s) 44, 144, 330
ErhI CCWWGG 3 cut(s) 44, 144, 330
FaeI CATG 3 cut(s) 142, 148, 264
FaiI YATR 3 cut(s) 140, 146, 262
FaqI GGGAC 1 cut(s) 278
FatI CATG 3 cut(s) 138, 144, 260
FauI CCCGC 1 cut(s) 236
Fnu4HI GCNGC 5 cut(s) 133, 136, 165, 258, 288
Fsp4HI GCNGC 5 cut(s) 133, 136, 165, 258, 288
GlaI GCGC 3 cut(s) 163, 211, 286
GluI GCNGC 5 cut(s) 133, 136, 165, 258, 288
GsuI CTGGAG 1 cut(s) 130
HaeII RGCGCY 2 cut(s) 213, 288
HaeIII GGCC 2 cut(s) 132, 154
HapII CCGG 2 cut(s) 24, 236
HhaI GCGC 3 cut(s) 164, 212, 287
Hin1II CATG 3 cut(s) 142, 148, 264
Hin6I GCGC 3 cut(s) 162, 210, 285
HinP1I GCGC 3 cut(s) 162, 210, 285
HpaII CCGG 2 cut(s) 24, 236
Hpy188I TCNGA 3 cut(s) 184, 229, 325
Hpy188III TCNNGA 1 cut(s) 205
Hpy99I CGWCG 1 cut(s) 368
HpyCH4III ACNGT 2 cut(s) 19, 180
HpyCH4IV ACGT 1 cut(s) 99
HpyF10VI GCNNNNNNNGC 3 cut(s) 207, 249, 303
HpyF3I CTNAG 1 cut(s) 281
HpySE526I ACGT 1 cut(s) 99
Hsp92II CATG 3 cut(s) 142, 148, 264
HspAI GCGC 3 cut(s) 162, 210, 285
KpnI GGTACC 1 cut(s) 77
LmnI GCTCC 1 cut(s) 277
LpnPI CCDG 8 cut(s) 22, 37, 94, 121, 168, 183, 235, 249
Lsp1109I GCAGC 2 cut(s) 176, 274
LweI GCATC 2 cut(s) 21, 210
MaeII ACGT 1 cut(s) 99
MfeI CAATTG 1 cut(s) 385
MluCI AATT 1 cut(s) 385
MmeI TCCRAC 1 cut(s) 348
MnlI CCTC 7 cut(s) 44, 64, 72, 133, 208, 262, 268
MslI CAYNNNNRTG 1 cut(s) 143
MspI CCGG 2 cut(s) 24, 236
MspR9I CCNGG 2 cut(s) 109, 236
MunI CAATTG 1 cut(s) 385
MvaI CCWGG 1 cut(s) 109
MvnI CGCG 1 cut(s) 306
MwoI GCNNNNNNNGC 3 cut(s) 207, 249, 303
NciI CCSGG 1 cut(s) 236
NcoI CCATGG 1 cut(s) 144
NlaIII CATG 3 cut(s) 142, 148, 264
NlaIV GGNNCC 1 cut(s) 75
NmeAIII GCCGAG 1 cut(s) 238
PkrI GCNGC 5 cut(s) 134, 137, 166, 259, 289
Psp1406I AACGTT 1 cut(s) 99
Psp6I CCWGG 1 cut(s) 107
PspGI CCWGG 1 cut(s) 107
PspN4I GGNNCC 1 cut(s) 75
RsaI GTAC 2 cut(s) 75, 376
RsaNI GTAC 2 cut(s) 74, 375
RseI CAYNNNNRTG 1 cut(s) 143
SatI GCNGC 5 cut(s) 133, 136, 165, 258, 288
ScrFI CCNGG 2 cut(s) 109, 236
SetI ASST 8 cut(s) 75, 102, 110, 119, 192, 224, 273, 282
SfaNI GCATC 2 cut(s) 21, 210
SmiMI CAYNNNNRTG 1 cut(s) 143
Sse9I AATT 1 cut(s) 385
SsiI CCGC 5 cut(s) 133, 136, 194, 243, 257
StyD4I CCNGG 2 cut(s) 107, 234
StyI CCWWGG 3 cut(s) 44, 144, 330
TaaI ACNGT 2 cut(s) 19, 180
TaiI ACGT 1 cut(s) 102
TaqI TCGA 1 cut(s) 59
TasI AATT 1 cut(s) 385
TauI GCSGC 3 cut(s) 135, 138, 260
TscAI CASTG 2 cut(s) 57, 162
TseI GCWGC 2 cut(s) 164, 287
TspGWI ACGGA 1 cut(s) 115
TspRI CASTG 2 cut(s) 57, 162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.