FvH4_5g39470

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0009318
Physical Location & Seq
Reverse (-)
29141465 .. 29145844
4380 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g39470.t1

Sequence Viewer

Length: 2169 bp
ATGGATAGTCTTTGTTTGAAAACCACGACGGGAATAGCTCCGACGATCACAGCCGTCGACGTCCGTACAAGCCCGTCGTCTCAGGTGAGCGCGCTGGGCAGGTCCACCCTCGACAAGCCCTTTTCCAGGTTCTCCTTCAATTACTACCCTTTGAAATCCCTCTGGCCCGGCGCCCGAAGAGCACCCGCTCCTACTAGTCCTACCTACAACGGGCTGCCTCTGGACGACGCCGTTCCATTGGCCCATGAGGAGGAGGAGGAGGAGGAATCCCAATCCCAAACACGTAGTGGGAACTGGGTTTTGAAGATTTTGCACGTCAGGTCTCTCTGGGGAGGAGGAGGAGGAGGAGGCAAGGCAGACCAAGTCGCAGAAGATAATAATGACGAAAATGACAGAAATGGTGATGATGACGAAAATGACGACGAGGACGAGTGTGATTGCTGTAGAATTGAGCAACAGGAGGATCATCAACAGGAGCAGGTTGAGTTTGATAGAGATTCGTTTTCCAGATTGCTTCGGAAGGTTTCGTTGGCCGAAGCCAGGTTATATGCTCAGATGTCGTATTTGGGCAACTTGGCTTACTCCATTCCCAAAATCCAGCCACAAAATCTCTTGAGACGTTATGCTCTGCGGTTTGTTACTTCTTCAATAGAAAAGAAGGAACTTGCTGCCAAAATTGAGAAACATCAAGAGTCACCTCCAATTGAAGATACCGAAGGCACCATAAAGGAAGAAATGGAAGATGACGAGGAAGGGAAGGAGCAGAAGAACAATGGTTACAGAATAAGTCCCTCTGCTGCTTATCATATTGCAGCCTCTGCAGCTTCCTATCTGCATTCCCATACAAGGAGTATACTTCCATTCAAATCCTCAAAGGATGAGACTGATCAAGATTCACCAGAAGAAAGCAGAGGAAGAGACAACGTTGTTAATATGATAAACTCAGACATGGCTTCTTTAATGGCAACCACGGACTCAGTAACTTCTGTGGTTGCTGCAAAAGAAGAAGTAAAGCAGGCTGTTGCAGACGATTTAAACTCAACACACTCATCCCCATGCGACTGGTTCATATGTGATGATGATCAAAGTGCCACCAGATTCTTGGTCATTCAGGGGTCTGAATCTCTGGCATCCTGGCAAGCAAATCTACTTTTTGAGCCCATTCAGTTCGAGGGACTGGATGTACTTGTACATAGAGGCATATACGAGGCTGCAAAAGGGATTTATGAGCAGGTGTTACCTGAAGTTCAGGGTCACCTCAAATCTCGTGGTGACAAAGCAACCTTCCGTTTTACTGGGCACTCATTAGGTGGAAGTTTAGCACTACTAATAAACCTCATGCTGCTTATACGGGGTGAAGTGCTGATCTCTTCTTTACTTCCTGTTATAACTTTTGGTTCACCATCCATCATGTGTGGAGGTGATCAACTGCTTCGCAAACTTGGATTAGCACGGAGTCATATCCAGGCAATTACATTGCACAGAGACATTGTACCCCGGGCATTCTCTTGCAATTATCCTAATCATGTGGCAGAGCTTCTCAAAGCTTTAAATGGGAACTTCCGGAATCATCCCTGCCTTAATAATCAGAAGCTACTTTATTCTCCAATGGGTGAGCTGTGGATTCTTCAACCAGATGAGAAATTCTCCCCAAACCATCATCTTCTACCTTCAGGCAGTGGTTTATATGTTCTAAGTGGTGCTTTGTCAGATGCCAATGACGCAGAGAAGCTGCTGCATGCAGCAAAGTTGGTATTCTTAAACTCACCACATCCACTTGAAATCTTAAGTGATCGGTCTGCATATGGTTCAGAAGGATGCATTCAAAGAGATCATGACATGAACTCTTACTTCAAATCTGTTCGTGGAGTGATTCGACAAGAGCTTAACCATATTAGGAAGGCCAGAAGACAGCAGAGGCGCAAGGTTTGGTGGCCCCTCGTCTCATCCCGGGGTGTGGATCTTGGAATCATTGTGGGAAGGCCTTTTGCGACAATGAAGCAGGGCCAGTACCAGGTCAATTTTTCTGGCATGTTACAAAGTGGGAAAGAGTCCTTGAAACGGTTCAGTAGACTGGTTGCATCACAGCACATGCATTTGTTTGTGGTGCTTTTGGTACCAGCACGACTCATACTCTTGGGGGCATATAGCGTGATTGGTTTCCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

723

Amino Acids

80.38

Weight (kDa)

5.78

Isoelectric Point (pI)

55.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 368 - 505 1.1e-26 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1388
AarI CACCTGC 1 cut(s) 1222
AatII GACGTC 1 cut(s) 63
Acc36I ACCTGC 3 cut(s) 90, 469, 1222
Acc65I GGTACC 1 cut(s) 2116
AccB1I GGYRCC 3 cut(s) 170, 719, 2116
AccBSI CCGCTC 1 cut(s) 188
AccI GTMKAC 3 cut(s) 57, 853, 2071
AccII CGCG 1 cut(s) 92
AccIII TCCGGA 1 cut(s) 1563
AciI CCGC 2 cut(s) 186, 631
AclI AACGTT 1 cut(s) 924
AclWI GGATC 2 cut(s) 471, 1968
AcoI YGGCCR 1 cut(s) 531
AcsI RAATTY 1 cut(s) 1643
AcuI CTGAAG 2 cut(s) 1263, 1656
AcyI GRCGYC 3 cut(s) 60, 171, 228
AdeI CACNNNGTG 1 cut(s) 287
AfaI GTAC 6 cut(s) 67, 1185, 1191, 1494, 2012, 2118
AfiI CCNNNNNNNGG 8 cut(s) 126, 210, 250, 540, 846, 1957, 2014, 2061
AflII CTTAAG 1 cut(s) 1786
AflIII ACRYGT 1 cut(s) 281
AhlI ACTAGT 1 cut(s) 194
AjiI CACGTC 1 cut(s) 316
AjnI CCWGG 5 cut(s) 125, 539, 1133, 1464, 2013
AjuI GAANNNNNNNTTGG 2 cut(s) 512, 544
AluBI AGCT 8 cut(s) 38, 824, 1537, 1547, 1594, 1618, 1732, 1885
AluI AGCT 8 cut(s) 38, 824, 1537, 1547, 1594, 1618, 1732, 1885
Alw21I GWGCWC 1 cut(s) 184
Alw26I GTCTC 7 cut(s) 84, 327, 610, 875, 912, 1479, 1948
AlwI GGATC 2 cut(s) 471, 1968
AlwNI CAGNNNCTG 1 cut(s) 818
Ama87I CYCGRG 2 cut(s) 1497, 1950
Aor13HI TCCGGA 1 cut(s) 1563
AoxI GGCC 7 cut(s) 164, 240, 531, 1902, 1934, 1982, 2005
ApoI RAATTY 1 cut(s) 1643
Asp700I GAANNNNTTC 1 cut(s) 2063
Asp718I GGTACC 1 cut(s) 2116
AspLEI GCGC 4 cut(s) 92, 94, 173, 1923
AspS9I GGNCC 5 cut(s) 102, 165, 241, 1935, 2005
AsuC2I CCSGG 5 cut(s) 168, 1498, 1499, 1951, 1952
AvaI CYCGRG 2 cut(s) 1497, 1950
AvaII GGWCC 1 cut(s) 102
BaeGI GKGCMC 1 cut(s) 1302
BanI GGYRCC 3 cut(s) 170, 719, 2116
BanII GRGCYC 1 cut(s) 1161
BauI CACGAG 1 cut(s) 1266
BbsI GAAGAC 1 cut(s) 1915
Bbv12I GWGCWC 1 cut(s) 184
BccI CCATC 3 cut(s) 1411, 1415, 1665
BceAI ACGGC 2 cut(s) 38, 215
BciT130I CCWGG 5 cut(s) 127, 541, 1135, 1466, 2015
BclI TGATCA 3 cut(s) 886, 1081, 1423
BcnI CCSGG 5 cut(s) 168, 1498, 1499, 1951, 1952
BcoDI GTCTC 7 cut(s) 84, 327, 610, 875, 912, 1479, 1948
BcuI ACTAGT 1 cut(s) 194
BfaI CTAG 1 cut(s) 195
BfmI CTRYAG 2 cut(s) 442, 819
BfoI RGCGCY 1 cut(s) 174
BfrI CTTAAG 1 cut(s) 1786
BfuAI ACCTGC 3 cut(s) 90, 469, 1222
Bme18I GGWCC 1 cut(s) 102
BmeT110I CYCGRG 2 cut(s) 1497, 1950
BmgBI CACGTC 1 cut(s) 316
BmgT120I GGNCC 5 cut(s) 102, 165, 241, 1935, 2005
BmiI GGNNCC 4 cut(s) 172, 721, 1937, 2118
BmrI ACTGGG 2 cut(s) 304, 1305
BmsI GCATC 4 cut(s) 1139, 1702, 1808, 2090
BmuI ACTGGG 2 cut(s) 304, 1305
BpiI GAAGAC 1 cut(s) 1915
BplI GAGNNNNNCTC 2 cut(s) 1631, 1663
BpuEI CTTGAG 1 cut(s) 634
BpuMI CCSGG 5 cut(s) 168, 1498, 1499, 1951, 1952
BsaAI YACGTR 1 cut(s) 284
BsaBI GATNNNNATC 1 cut(s) 1080
BsaHI GRCGYC 3 cut(s) 60, 171, 228
BsaI GGTCTC 1 cut(s) 327
BsaJI CCNNGG 5 cut(s) 969, 1496, 1497, 1950, 1951
BsaWI WCCGGW 1 cut(s) 1563
Bsc4I CCNNNNNNNGG 8 cut(s) 126, 210, 250, 540, 846, 1957, 2014, 2061
Bse1I ACTGG 6 cut(s) 299, 1067, 1182, 1300, 2008, 2079
Bse3DI GCAATG 1 cut(s) 1475
Bse8I GATNNNNATC 1 cut(s) 1080
BseAI TCCGGA 1 cut(s) 1563
BseBI CCWGG 5 cut(s) 127, 541, 1135, 1466, 2015
BseDI CCNNGG 5 cut(s) 969, 1496, 1497, 1950, 1951
BseGI GGATG 9 cut(s) 883, 1049, 1130, 1186, 1403, 1570, 1771, 1823, 1946
BseJI GATNNNNATC 1 cut(s) 1080
BseLI CCNNNNNNNGG 8 cut(s) 126, 210, 250, 540, 846, 1957, 2014, 2061
BseMI GCAATG 1 cut(s) 1475
BseMII CTCAG 4 cut(s) 95, 566, 957, 990
BseNI ACTGG 6 cut(s) 299, 1067, 1182, 1300, 2008, 2079
BsePI GCGCGC 1 cut(s) 90
BseSI GKGCMC 1 cut(s) 1302
BseYI CCCAGC 1 cut(s) 94
Bsh1236I CGCG 1 cut(s) 92
BshFI GGCC 7 cut(s) 166, 242, 533, 1904, 1936, 1984, 2007
BshNI GGYRCC 3 cut(s) 170, 719, 2116
BsiHKAI GWGCWC 1 cut(s) 184
BsiHKCI CYCGRG 2 cut(s) 1497, 1950
BsiSI CCGG 4 cut(s) 168, 1498, 1564, 1951
BslFI GGGAC 2 cut(s) 774, 1188
BslI CCNNNNNNNGG 8 cut(s) 126, 210, 250, 540, 846, 1957, 2014, 2061
BsmAI GTCTC 7 cut(s) 84, 327, 610, 875, 912, 1479, 1948
BsmBI CGTCTC 3 cut(s) 84, 610, 1948
BsmFI GGGAC 2 cut(s) 774, 1188
BsmI GAATGC 3 cut(s) 835, 1502, 1821
BsnI GGCC 7 cut(s) 166, 242, 533, 1904, 1936, 1984, 2007
Bso31I GGTCTC 1 cut(s) 327
BsoBI CYCGRG 2 cut(s) 1497, 1950
Bsp1286I GDGCHC 3 cut(s) 184, 1161, 1302
Bsp13I TCCGGA 1 cut(s) 1563
Bsp1407I TGTACA 1 cut(s) 1189
Bsp143I GATC 9 cut(s) 45, 463, 886, 1081, 1365, 1423, 1792, 1831, 1960
BspACI CCGC 2 cut(s) 186, 631
BspANI GGCC 7 cut(s) 166, 242, 533, 1904, 1936, 1984, 2007
BspCNI CTCAG 4 cut(s) 94, 565, 956, 989
BspEI TCCGGA 1 cut(s) 1563
BspFNI CGCG 1 cut(s) 92
BspHI TCATGA 1 cut(s) 1834
BspLI GGNNCC 4 cut(s) 172, 721, 1937, 2118
BspMAI CTGCAG 1 cut(s) 823
BspMI ACCTGC 3 cut(s) 90, 469, 1222
BspPI GGATC 2 cut(s) 471, 1968
BspQI GCTCTTC 1 cut(s) 172
BspT107I GGYRCC 3 cut(s) 170, 719, 2116
BspTI CTTAAG 1 cut(s) 1786
BspTNI GGTCTC 1 cut(s) 327
BsrBI CCGCTC 1 cut(s) 188
BsrDI GCAATG 1 cut(s) 1475
BsrGI TGTACA 1 cut(s) 1189
BsrI ACTGG 6 cut(s) 299, 1067, 1182, 1300, 2008, 2079
BssECI CCNNGG 5 cut(s) 969, 1496, 1497, 1950, 1951
BssHII GCGCGC 1 cut(s) 90
BssMI GATC 9 cut(s) 45, 463, 886, 1081, 1365, 1423, 1792, 1831, 1960
BssNAI GTATAC 1 cut(s) 854
BssNI GRCGYC 3 cut(s) 60, 171, 228
BssSI CACGAG 1 cut(s) 1266
Bst1107I GTATAC 1 cut(s) 854
Bst2BI CACGAG 1 cut(s) 1266
Bst2UI CCWGG 5 cut(s) 127, 541, 1135, 1466, 2015
Bst4CI ACNGT 1 cut(s) 2064
Bst6I CTCTTC 3 cut(s) 172, 910, 1375
BstACI GRCGYC 3 cut(s) 60, 171, 228
BstAFI CTTAAG 1 cut(s) 1786
BstAPI GCANNNNNTGC 1 cut(s) 818
BstAUI TGTACA 1 cut(s) 1189
BstBAI YACGTR 1 cut(s) 284
BstC8I GCNNGC 4 cut(s) 92, 1017, 1140, 1740
BstDEI CTNAG 5 cut(s) 81, 552, 943, 976, 1694
BstDSI CCRYGG 1 cut(s) 969
BstEII GGTNACC 1 cut(s) 1253
BstENI CCTNNNNNAGG 1 cut(s) 124
BstF5I GGATG 9 cut(s) 883, 1049, 1130, 1186, 1403, 1570, 1771, 1823, 1946
BstFNI CGCG 1 cut(s) 92
BstH2I RGCGCY 1 cut(s) 174
BstHHI GCGC 4 cut(s) 92, 94, 173, 1923
BstKTI GATC 9 cut(s) 48, 466, 889, 1084, 1368, 1426, 1795, 1834, 1963
BstMAI GTCTC 7 cut(s) 84, 327, 610, 875, 912, 1479, 1948
BstMBI GATC 9 cut(s) 45, 463, 886, 1081, 1365, 1423, 1792, 1831, 1960
BstMWI GCNNNNNNNGC 5 cut(s) 96, 179, 818, 821, 1721
BstNI CCWGG 5 cut(s) 127, 541, 1135, 1466, 2015
BstNSI RCATGY 3 cut(s) 1742, 2035, 2095
BstPI GGTNACC 1 cut(s) 1253
BstSFI CTRYAG 2 cut(s) 442, 819
BstSLI GKGCMC 1 cut(s) 1302
BstUI CGCG 1 cut(s) 92
BstV2I GAAGAC 1 cut(s) 1915
BstX2I RGATCY 1 cut(s) 1960
BstXI CCANNNNNNTGG 2 cut(s) 1062, 1102
BstYI RGATCY 1 cut(s) 1960
BstZ17I GTATAC 1 cut(s) 854
BsuRI GGCC 7 cut(s) 166, 242, 533, 1904, 1936, 1984, 2007
BtgI CCRYGG 1 cut(s) 969
BtrI CACGTC 1 cut(s) 316
BtsCI GGATG 9 cut(s) 883, 1049, 1130, 1186, 1403, 1570, 1771, 1823, 1946
BtsI GCAGTG 1 cut(s) 1684
BtsIMutI CAGTG 1 cut(s) 1684
BveI ACCTGC 3 cut(s) 90, 469, 1222
Cac8I GCNNGC 4 cut(s) 92, 1017, 1140, 1740
CaiI CAGNNNCTG 1 cut(s) 818
CciI TCATGA 1 cut(s) 1834
CfoI GCGC 4 cut(s) 92, 94, 173, 1923
Cfr13I GGNCC 5 cut(s) 102, 165, 241, 1935, 2005
Cfr9I CCCGGG 2 cut(s) 1497, 1950
CseI GACGC 2 cut(s) 236, 1730
CsiI ACCWGGT 1 cut(s) 2013
Csp6I GTAC 6 cut(s) 66, 1184, 1190, 1493, 2011, 2117
CspCI CAANNNNNGTGG 2 cut(s) 1249, 1284
CviQI GTAC 6 cut(s) 66, 1184, 1190, 1493, 2011, 2117
DdeI CTNAG 5 cut(s) 81, 552, 943, 976, 1694
DinI GGCGCC 1 cut(s) 172
DpnI GATC 9 cut(s) 47, 465, 888, 1083, 1367, 1425, 1794, 1833, 1962
DpnII GATC 9 cut(s) 45, 463, 886, 1081, 1365, 1423, 1792, 1831, 1960
DraI TTTAAA 2 cut(s) 1035, 1551
DraIII CACNNNGTG 1 cut(s) 287
EaeI YGGCCR 1 cut(s) 531
Eam1104I CTCTTC 3 cut(s) 172, 910, 1375
EarI CTCTTC 3 cut(s) 172, 910, 1375
Eco147I AGGCCT 1 cut(s) 1984
Eco24I GRGCYC 1 cut(s) 1161
Eco31I GGTCTC 1 cut(s) 327
Eco47I GGWCC 1 cut(s) 102
Eco57I CTGAAG 2 cut(s) 1263, 1656
Eco88I CYCGRG 2 cut(s) 1497, 1950
Eco91I GGTNACC 1 cut(s) 1253
EcoNI CCTNNNNNAGG 1 cut(s) 124
EcoO65I GGTNACC 1 cut(s) 1253
EcoRII CCWGG 5 cut(s) 125, 539, 1133, 1464, 2013
EcoT22I ATGCAT 2 cut(s) 1823, 2097
EcoT38I GRGCYC 1 cut(s) 1161
EgeI GGCGCC 1 cut(s) 172
EheI GGCGCC 1 cut(s) 172
Esp3I CGTCTC 3 cut(s) 84, 610, 1948
FalI AAGNNNNNCTT 2 cut(s) 1687, 1719
FaqI GGGAC 2 cut(s) 774, 1188
FauI CCCGC 1 cut(s) 193
FauNDI CATATG 2 cut(s) 1070, 1804
FbaI TGATCA 3 cut(s) 886, 1081, 1423
FblI GTMKAC 3 cut(s) 57, 853, 2071
FokI GGATG 9 cut(s) 890, 1036, 1117, 1193, 1390, 1557, 1758, 1830, 1933
FriOI GRGCYC 1 cut(s) 1161
FspBI CTAG 1 cut(s) 195
GlaI GCGC 4 cut(s) 91, 93, 172, 1922
GsaI CCCAGC 1 cut(s) 98
HaeII RGCGCY 1 cut(s) 174
HaeIII GGCC 7 cut(s) 166, 242, 533, 1904, 1936, 1984, 2007
HapII CCGG 4 cut(s) 168, 1498, 1564, 1951
HgaI GACGC 2 cut(s) 236, 1730
HhaI GCGC 4 cut(s) 92, 94, 173, 1923
Hin1I GRCGYC 3 cut(s) 60, 171, 228
Hin6I GCGC 4 cut(s) 90, 92, 171, 1921
HinP1I GCGC 4 cut(s) 90, 92, 171, 1921
HincII GTYRAC 1 cut(s) 58
HindII GTYRAC 1 cut(s) 58
HindIII AAGCTT 1 cut(s) 1545
HpaII CCGG 4 cut(s) 168, 1498, 1564, 1951
Hpy166II GTNNAC 5 cut(s) 58, 105, 854, 1400, 2072
Hpy188I TCNGA 8 cut(s) 42, 519, 555, 946, 1120, 1590, 1711, 1813
Hpy188III TCNNGA 7 cut(s) 221, 507, 613, 689, 890, 1564, 1835
Hpy8I GTNNAC 5 cut(s) 58, 105, 854, 1400, 2072
Hpy99I CGWCG 7 cut(s) 31, 46, 59, 62, 79, 230, 425
HpyCH4III ACNGT 1 cut(s) 2064
HpyCH4IV ACGT 5 cut(s) 60, 283, 315, 619, 924
HpyF10VI GCNNNNNNNGC 5 cut(s) 96, 179, 818, 821, 1721
HpyF3I CTNAG 5 cut(s) 81, 552, 943, 976, 1694
HpySE526I ACGT 5 cut(s) 60, 283, 315, 619, 924
Hsp92I GRCGYC 3 cut(s) 60, 171, 228
HspAI GCGC 4 cut(s) 90, 92, 171, 1921
KasI GGCGCC 1 cut(s) 170
Kpn2I TCCGGA 1 cut(s) 1563
KpnI GGTACC 1 cut(s) 2120
Ksp22I TGATCA 3 cut(s) 886, 1081, 1423
Kzo9I GATC 9 cut(s) 45, 463, 886, 1081, 1365, 1423, 1792, 1831, 1960
LguI GCTCTTC 1 cut(s) 172
LmnI GCTCC 4 cut(s) 43, 193, 475, 760
LweI GCATC 4 cut(s) 1139, 1702, 1808, 2090
MabI ACCWGGT 1 cut(s) 2013
MaeI CTAG 1 cut(s) 195
MaeII ACGT 5 cut(s) 60, 283, 315, 619, 924
MaeIII GTNAC 8 cut(s) 637, 693, 776, 979, 1236, 1253, 1271, 2034
MalI GATC 9 cut(s) 47, 465, 888, 1083, 1367, 1425, 1794, 1833, 1962
MbiI CCGCTC 1 cut(s) 188
MboI GATC 9 cut(s) 45, 463, 886, 1081, 1365, 1423, 1792, 1831, 1960
MfeI CAATTG 1 cut(s) 702
MflI RGATCY 1 cut(s) 1960
MhlI GDGCHC 3 cut(s) 184, 1161, 1302
MluCI AATT 8 cut(s) 139, 447, 675, 702, 1470, 1513, 1643, 2020
Mly113I GGCGCC 1 cut(s) 171
MlyI GAGTC 5 cut(s) 701, 968, 1465, 2060, 2121
MmeI TCCRAC 1 cut(s) 65
Mph1103I ATGCAT 2 cut(s) 1823, 2097
MroI TCCGGA 1 cut(s) 1563
MroXI GAANNNNTTC 1 cut(s) 2063
MseI TTAA 8 cut(s) 930, 959, 1034, 1550, 1581, 1760, 1787, 1887
MslI CAYNNNNRTG 1 cut(s) 1054
MspCI CTTAAG 1 cut(s) 1786
MspI CCGG 4 cut(s) 168, 1498, 1564, 1951
MunI CAATTG 1 cut(s) 702
Mva1269I GAATGC 3 cut(s) 835, 1502, 1821
MvaI CCWGG 5 cut(s) 127, 541, 1135, 1466, 2015
MvnI CGCG 1 cut(s) 92
MwoI GCNNNNNNNGC 5 cut(s) 96, 179, 818, 821, 1721
NarI GGCGCC 1 cut(s) 171
NciI CCSGG 5 cut(s) 168, 1498, 1499, 1951, 1952
NdeI CATATG 2 cut(s) 1070, 1804
NdeII GATC 9 cut(s) 45, 463, 886, 1081, 1365, 1423, 1792, 1831, 1960
NlaIV GGNNCC 4 cut(s) 172, 721, 1937, 2118
NmuCI GTSAC 3 cut(s) 693, 1253, 1271
NsiI ATGCAT 2 cut(s) 1823, 2097
NspI RCATGY 3 cut(s) 1742, 2035, 2095
PaeI GCATGC 1 cut(s) 1742
PagI TCATGA 1 cut(s) 1834
PaqCI CACCTGC 1 cut(s) 1222
PauI GCGCGC 1 cut(s) 90
PceI AGGCCT 1 cut(s) 1984
PciSI GCTCTTC 1 cut(s) 172
PcsI WCGNNNNNNNCGW 2 cut(s) 417, 426
PctI GAATGC 3 cut(s) 835, 1502, 1821
PdmI GAANNNNTTC 1 cut(s) 2063
PfeI GAWTC 9 cut(s) 266, 497, 893, 1098, 1121, 1567, 1624, 1873, 1968
PflFI GACNNNGTC 1 cut(s) 362
PleI GAGTC 5 cut(s) 700, 968, 1464, 2059, 2121
PluTI GGCGCC 1 cut(s) 174
PpsI GAGTC 5 cut(s) 700, 968, 1464, 2059, 2121
Ppu21I YACGTR 1 cut(s) 284
PsiI TTATAA 1 cut(s) 1388
Psp1406I AACGTT 1 cut(s) 924
Psp6I CCWGG 5 cut(s) 125, 539, 1133, 1464, 2013
PspEI GGTNACC 1 cut(s) 1253
PspFI CCCAGC 1 cut(s) 94
PspGI CCWGG 5 cut(s) 125, 539, 1133, 1464, 2013
PspN4I GGNNCC 4 cut(s) 172, 721, 1937, 2118
PspPI GGNCC 5 cut(s) 102, 165, 241, 1935, 2005
PsrI GAACNNNNNNTAC 2 cut(s) 761, 793
PstI CTGCAG 1 cut(s) 823
PstNI CAGNNNCTG 1 cut(s) 818
PsuI RGATCY 1 cut(s) 1960
PsyI GACNNNGTC 1 cut(s) 362
PteI GCGCGC 1 cut(s) 90
RsaI GTAC 6 cut(s) 67, 1185, 1191, 1494, 2012, 2118
RsaNI GTAC 6 cut(s) 66, 1184, 1190, 1493, 2011, 2117
RseI CAYNNNNRTG 1 cut(s) 1054
SalI GTCGAC 1 cut(s) 56
SapI GCTCTTC 1 cut(s) 172
SaqAI TTAA 8 cut(s) 930, 959, 1034, 1550, 1581, 1760, 1787, 1887
Sau3AI GATC 9 cut(s) 45, 463, 886, 1081, 1365, 1423, 1792, 1831, 1960
Sau96I GGNCC 5 cut(s) 102, 165, 241, 1935, 2005
SchI GAGTC 5 cut(s) 701, 968, 1465, 2060, 2121
SduI GDGCHC 3 cut(s) 184, 1161, 1302
SexAI ACCWGGT 1 cut(s) 2013
SfaNI GCATC 4 cut(s) 1139, 1702, 1808, 2090
SfcI CTRYAG 2 cut(s) 442, 819
SfoI GGCGCC 1 cut(s) 172
SgrDI CGTCGACG 1 cut(s) 56
SinI GGWCC 1 cut(s) 102
SmaI CCCGGG 2 cut(s) 1499, 1952
SmiMI CAYNNNNRTG 1 cut(s) 1054
SmlI CTYRAG 2 cut(s) 613, 1786
SmoI CTYRAG 2 cut(s) 613, 1786
SpeI ACTAGT 1 cut(s) 194
SphI GCATGC 1 cut(s) 1742
Sse9I AATT 8 cut(s) 139, 447, 675, 702, 1470, 1513, 1643, 2020
SseBI AGGCCT 1 cut(s) 1984
SsiI CCGC 2 cut(s) 186, 631
SspDI GGCGCC 1 cut(s) 170
SspMI CTAG 1 cut(s) 195
StuI AGGCCT 1 cut(s) 1984
TaaI ACNGT 1 cut(s) 2064
TaiI ACGT 5 cut(s) 63, 286, 318, 622, 927
TaqI TCGA 4 cut(s) 57, 111, 1170, 1876
TaqII GACCGA 1 cut(s) 1785
TasI AATT 8 cut(s) 139, 447, 675, 702, 1470, 1513, 1643, 2020
TatI WGTACW 2 cut(s) 1183, 1189
TfiI GAWTC 9 cut(s) 266, 497, 893, 1098, 1121, 1567, 1624, 1873, 1968
Tru1I TTAA 8 cut(s) 930, 959, 1034, 1550, 1581, 1760, 1787, 1887
Tru9I TTAA 8 cut(s) 930, 959, 1034, 1550, 1581, 1760, 1787, 1887
TscAI CASTG 1 cut(s) 1684
TseFI GTSAC 3 cut(s) 693, 1253, 1271
Tsp45I GTSAC 3 cut(s) 693, 1253, 1271
TspDTI ATGAA 3 cut(s) 1057, 1856, 2012
TspGWI ACGGA 4 cut(s) 53, 986, 1277, 1468
TspMI CCCGGG 2 cut(s) 1497, 1950
TspRI CASTG 1 cut(s) 1684
Tth111I GACNNNGTC 1 cut(s) 362
Vha464I CTTAAG 1 cut(s) 1786
VpaK11BI GGWCC 1 cut(s) 102
XagI CCTNNNNNAGG 1 cut(s) 124
XapI RAATTY 1 cut(s) 1643
XceI RCATGY 3 cut(s) 1742, 2035, 2095
XcmI CCANNNNNNNNNTGG 2 cut(s) 284, 1099
XmaI CCCGGG 2 cut(s) 1497, 1950
XmiI GTMKAC 3 cut(s) 57, 853, 2071
XmnI GAANNNNTTC 1 cut(s) 2063
XspI CTAG 1 cut(s) 195
ZraI GACGTC 1 cut(s) 61
Zsp2I ATGCAT 2 cut(s) 1823, 2097
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.