Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr11

Orthogroup ID

OG0009318

Physical Location & Seq

Genomic Coordinates Reverse (-)

29827077 .. 29830421

Gene Structure 3345 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom11g27620.4

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2247 bp

ATGGATGGTCTTTGTCTGAAAACAACTGCGCCAGGGATACACGGCCTATCTTCCAACATCTCAGCGGCTGCAGCCCTCGATACCCGTACGAGCCCATCTCCGTCTCAGGTGAGCGCCATTGGCTGCTCCTCCGCATCTGGGATTGACAAGTCAACCGTAACCGCGGCTCAGAAAAGGACAACTTCGTTTTCCGGATTCTCATTCAAGCACCCTCTGAAATCCTTATGGCCTGGAGCAGCAAGAGGAGGTGGACCCGCCTACAATGGGATGGCTCTCGACGACGCCGTCCCCTTGTCCTCTAAGGAGGAGGGCGAGCAGGAGGAGAAGGAGGAGGAGCTCCAGAGTGCGACCAGGGCGTCGGACGGTCAAAGTGGGAACTGGGTTTTGAAGATTTTGCACGTGAGGTCTCTCTCTCTACCCAGGAGAGAGGAACAGCAGGGAGGCAAGAGTGGCGGCGTTGCAGAGCTACCCACGGGAGATAGAAAAACATCGGTGGATGATGACTCGGAGGAAGACGATGACGACAACAATTGTTGTAGTGTTTCAGATGAGGCCGAGTTTGATCAAGACACGTTTTGTCGGTTGCTTCGGAAGGTCTCGTTGGCGGAGGCGAGGTTGTATGCTCAAATGTCGTATTTGGGGAGCTTAGCTTATTGCATTCCTAAAATCCAGACAGGAAATCTTCTAAGAAGTTATGGTCTGCGTTTTGTTACTTCTTCAGTAGAGAAGAAGGAATTGACTACTAAAACTGAGAAAGATCAGGAGTCAGCTGAGATTCGAGAAACTGAAAAAAACCTTAAGGAAGACAAGGAAGAAGATGCCGAAGTAAAGGAGCAAAAGAACTATGGATATCGAATAAGTCCATCTGCTGCTTATCAAATTGCTGCTTCTGCAGCTTCCTATCTGCATTCTCAAACAACGAGCATACTTCCATTCAAATCCTCAGAGACCGAGGCAGAAAAAGACTACCCTGAAGAAGGCAGTGAAAGAAGTGAAGCTGTTAATAGGATGAACTCAGAAGTGGCTTCTTTAATGGCAACCACCGACTCAGTTACAGCTGTGGTTGCTGCAAAGGAAGAAGTAAAGCAGGCTGTTGCAGATGACTTAAATTCAACAATTTCATCACCTTGTGAATGGTTCGTATGTGATGATGATCGAAGTGGCACCAGATTCTTTGTCATTCAGGGGTCTGAATCACTAGCATCCTGGCAAGCAAATCTACTTTTTGAGCCCATTCAGTTTGAGGGACTGGATGTGCTTGTGCATAGAGGCATATACGAGGCAGCGAAAGGGATATACAAACAGATGCTGCCTGAAGTCCATGCTCACCTCAAATCTCATGGTGACCATGCAACCTTTCGTTATACTGGGCATTCTTTAGGGGGAAGCTTGGCACTCTTAATAAATCTCATGTTGCTTATACGGCTAGAAGTGCCAATTTCTTCCCTGCTTCCTGTTATTACTTTTGGTGCACCATCCATTATGTGTGAAGGTGATCGATTGCTTCGCAAGCTTGGATTACCGCGAAGTCATGTTCAGTCTATTACGTTGCACAGAGACATTGTACCCCGTGCCTTCTCTTGCAATTATCCAAATCATGTGGCAGAGCTTCTCAAGGCTTTGAATGGGAACTTTCGGAATCATCCCTGTCTCAATAACCAGAAGCTACTGTATTCTCCAATGGGGGAGATCCTGATTCTTCAACCAGATGAGAAATTTTCCCCAAATCATCATCTTCTTCCAGCAGGCAGCGGTCTGTATCTTCTAAGCTGTCCTCTGTCTGATGGCAGTGATGCAGAGAAGCAGCTCCGCGCTGCACAGTTGGTATTCCTAAACTCACCACACCCACTTGAAATCTTAAGTGATCGGTCTGCATATGGTTCAGAAGGAACCATTCAAAGAGATCATGATGTGAATTCTTACTTAAAATCTGTTCGCGGGGTAATTCGGGAAGAGCTTAGCCAGATCAGAAAGGCCAGGAGACAGCAGCGCCGCAAGGTGTGGTGGCCCTTGATAGCACCCCGAAGCAAGCATGCAGGAGGTGTGATTGTGGGAAGGACTGTTGCCTCAATCAACCTGGGGCAAGAAGAGCAATTAAACTTTTCGGGCGCAGTACAGACGGGAAAAGAAACGTTGAAACGGTTCAGTAGACTTGTTGCATGGGAGCACATGCATTTGTTTGTGGTGCTTTTGGTACCGGCGCGATTGTTAGTCTTGGGGGCATACAGCATCATCAGTTTCCGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Lipase_3

Protein Analysis

749

Amino Acids

82.2

Weight (kDa)

6.01

Isoelectric Point (pI)

46.21

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009318)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G02660
fragaria_vesca	FvH4_5g39470 FvH4_5g39470 FvH4_5g39470
malus_domestica	MD03G1296900.v1.1 MD11G1316500.v1.1
prunus_persica	Prupe.8G270300_v2.0.a1
pyrus_communis	pycom03g23540 pycom11g27620
rosa_chinensis	RchiOBHm_Chr7g0241801
rosa_laevigata	RLG00000000543
rosa_multiflora	Rmu_sc0010095.1_g000001 Rmu_sc0042388.1_g000001
rosa_roxburghii	Rroxscaffold_3G00218670
rosa_rugosa	Rorug07G0347200 Rorug07G0347200 Rorug07G0347300
rosa_samantha	Rh7AG501700 Rh7BG473000 Rh7CG518000 Rh7DG486900
rosa_wichuraiana	Rw7G042410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	284, 355
Acc65I	GGTACC	1 cut(s)	2194
AccB1I	GGYRCC	2 cut(s)	1163, 2194
AccI	GTMKAC	1 cut(s)	2149
AccII	CGCG	5 cut(s)	164, 1525, 1812, 1938, 2203
AccIII	TCCGGA	1 cut(s)	191
AclI	AACGTT	1 cut(s)	2132
AclWI	GGATC	1 cut(s)	1684
AcsI	RAATTY	3 cut(s)	1108, 1715, 1915
AcuI	CTGAAG	3 cut(s)	702, 993, 1335
AcvI	CACGTG	1 cut(s)	400
AcyI	GRCGYC	2 cut(s)	282, 356
AdeI	CACNNNGTG	1 cut(s)	1130
AfaI	GTAC	4 cut(s)	88, 1566, 2115, 2196
AfiI	CCNNNNNNNGG	3 cut(s)	138, 264, 977
AflII	CTTAAG	2 cut(s)	797, 1858
AflIII	ACRYGT	1 cut(s)	570
AgsI	TTSAA	9 cut(s)	205, 388, 937, 1113, 1624, 1703, 1853, 1898, 2137
AjnI	CCWGG	7 cut(s)	31, 229, 350, 419, 1205, 1976, 2076
AjuI	GAANNNNNNNTTGG	2 cut(s)	584, 616
Alw21I	GWGCWC	3 cut(s)	339, 1474, 2169
Alw26I	GTCTC	7 cut(s)	108, 411, 601, 941, 1551, 1655, 1975
Alw44I	GTGCAC	1 cut(s)	1470
AlwI	GGATC	1 cut(s)	1684
AlwNI	CAGNNNCTG	2 cut(s)	68, 1309
Aor13HI	TCCGGA	1 cut(s)	191
AoxI	GGCC	5 cut(s)	43, 227, 552, 1974, 2006
ApaLI	GTGCAC	1 cut(s)	1470
ApoI	RAATTY	3 cut(s)	1108, 1715, 1915
Asp700I	GAANNNNTTC	1 cut(s)	2141
Asp718I	GGTACC	1 cut(s)	2194
AspLEI	GCGC	6 cut(s)	31, 116, 1814, 1992, 2111, 2203
AspS9I	GGNCC	2 cut(s)	251, 2007
AsuHPI	GGTGA	6 cut(s)	121, 1116, 1319, 1355, 1505, 1830
AvaII	GGWCC	1 cut(s)	251
BaeGI	GKGCMC	1 cut(s)	1474
BanI	GGYRCC	2 cut(s)	1163, 2194
BanII	GRGCYC	3 cut(s)	95, 339, 1233
BbrPI	CACGTG	1 cut(s)	400
BbsI	GAAGAC	2 cut(s)	519, 810
Bbv12I	GWGCWC	3 cut(s)	339, 1474, 2169
BccI	CCATC	5 cut(s)	103, 262, 871, 1483, 1778
BceAI	ACGGC	3 cut(s)	58, 269, 1439
BciT130I	CCWGG	7 cut(s)	33, 231, 352, 421, 1207, 1978, 2078
BciVI	GTATCC	1 cut(s)	30
BclI	TGATCA	1 cut(s)	562
BcoDI	GTCTC	7 cut(s)	108, 411, 601, 941, 1551, 1655, 1975
BfaI	CTAG	2 cut(s)	1199, 1427
BfmI	CTRYAG	2 cut(s)	69, 891
BfoI	RGCGCY	2 cut(s)	117, 1993
BfrI	CTTAAG	2 cut(s)	797, 1858
BfuI	GTATCC	1 cut(s)	30
BlpI	GCTNAGC	2 cut(s)	646, 1958
Bme1390I	CCNGG	7 cut(s)	33, 231, 352, 421, 1207, 1978, 2078
Bme18I	GGWCC	1 cut(s)	251
BmgT120I	GGNCC	2 cut(s)	251, 2007
BmiI	GGNNCC	4 cut(s)	253, 1165, 1891, 2196
BmrFI	CCNGG	7 cut(s)	33, 231, 352, 421, 1207, 1978, 2078
BmrI	ACTGGG	2 cut(s)	388, 1377
BmsI	GCATC	6 cut(s)	143, 808, 1211, 1296, 1783, 2238
BmuI	ACTGGG	2 cut(s)	388, 1377
BpiI	GAAGAC	2 cut(s)	519, 810
BplI	GAGNNNNNCTC	2 cut(s)	82, 114
BpmI	CTGGAG	2 cut(s)	252, 323
Bpu1102I	GCTNAGC	2 cut(s)	646, 1958
BpuEI	CTTGAG	1 cut(s)	1598
Bsa29I	ATCGAT	1 cut(s)	1498
BsaAI	YACGTR	1 cut(s)	400
BsaBI	GATNNNNATC	1 cut(s)	1152
BsaHI	GRCGYC	2 cut(s)	282, 356
BsaI	GGTCTC	3 cut(s)	411, 601, 941
BsaJI	CCNNGG	7 cut(s)	32, 162, 351, 419, 471, 951, 2077
BsaWI	WCCGGW	2 cut(s)	191, 2241
BsaXI	ACNNNNNCTCC	2 cut(s)	599, 629
Bsc4I	CCNNNNNNNGG	3 cut(s)	138, 264, 977
Bse118I	RCCGGY	1 cut(s)	2197
Bse1I	ACTGG	3 cut(s)	383, 1254, 1372
Bse8I	GATNNNNATC	1 cut(s)	1152
BseAI	TCCGGA	1 cut(s)	191
BseBI	CCWGG	7 cut(s)	33, 231, 352, 421, 1207, 1978, 2078
BseCI	ATCGAT	1 cut(s)	1498
BseDI	CCNNGG	7 cut(s)	32, 162, 351, 419, 471, 951, 2077
BseGI	GGATG	8 cut(s)	10, 273, 502, 1014, 1202, 1258, 1475, 1642
BseJI	GATNNNNATC	1 cut(s)	1152
BseLI	CCNNNNNNNGG	3 cut(s)	138, 264, 977
BseMII	CTCAG	8 cut(s)	75, 119, 182, 741, 762, 957, 1029, 1062
BseNI	ACTGG	3 cut(s)	383, 1254, 1372
BseRI	GAGGAG	6 cut(s)	118, 258, 320, 335, 344, 347
BseSI	GKGCMC	1 cut(s)	1474
BsgI	GTGCAG	1 cut(s)	1800
Bsh1236I	CGCG	5 cut(s)	164, 1525, 1812, 1938, 2203
BshFI	GGCC	5 cut(s)	45, 229, 554, 1976, 2008
BshNI	GGYRCC	2 cut(s)	1163, 2194
BshVI	ATCGAT	1 cut(s)	1498
BsiHKAI	GWGCWC	3 cut(s)	339, 1474, 2169
BsiSI	CCGG	3 cut(s)	192, 2198, 2242
BsiWI	CGTACG	1 cut(s)	86
BslFI	GGGAC	2 cut(s)	272, 1260
BslI	CCNNNNNNNGG	3 cut(s)	138, 264, 977
BsmAI	GTCTC	7 cut(s)	108, 411, 601, 941, 1551, 1655, 1975
BsmBI	CGTCTC	1 cut(s)	108
BsmFI	GGGAC	2 cut(s)	272, 1260
BsmI	GAATGC	3 cut(s)	657, 907, 1372
BsnI	GGCC	5 cut(s)	45, 229, 554, 1976, 2008
Bso31I	GGTCTC	3 cut(s)	411, 601, 941
Bsp1286I	GDGCHC	5 cut(s)	95, 339, 1233, 1474, 2169
Bsp13I	TCCGGA	1 cut(s)	191
Bsp143I	GATC	8 cut(s)	562, 757, 1153, 1495, 1689, 1864, 1903, 1965
Bsp1720I	GCTNAGC	2 cut(s)	646, 1958
BspANI	GGCC	5 cut(s)	45, 229, 554, 1976, 2008
BspCNI	CTCAG	8 cut(s)	74, 118, 181, 742, 763, 956, 1028, 1061
BspDI	ATCGAT	1 cut(s)	1498
BspEI	TCCGGA	1 cut(s)	191
BspFNI	CGCG	5 cut(s)	164, 1525, 1812, 1938, 2203
BspHI	TCATGA	1 cut(s)	1906
BspLI	GGNNCC	4 cut(s)	253, 1165, 1891, 2196
BspMAI	CTGCAG	2 cut(s)	73, 895
BspPI	GGATC	1 cut(s)	1684
BspQI	GCTCTTC	2 cut(s)	1947, 2082
BspT107I	GGYRCC	2 cut(s)	1163, 2194
BspTI	CTTAAG	2 cut(s)	797, 1858
BspTNI	GGTCTC	3 cut(s)	411, 601, 941
BsrFI	RCCGGY	1 cut(s)	2197
BsrI	ACTGG	3 cut(s)	383, 1254, 1372
BssAI	RCCGGY	1 cut(s)	2197
BssECI	CCNNGG	7 cut(s)	32, 162, 351, 419, 471, 951, 2077
BssMI	GATC	8 cut(s)	562, 757, 1153, 1495, 1689, 1864, 1903, 1965
BssNI	GRCGYC	2 cut(s)	282, 356
Bst2UI	CCWGG	7 cut(s)	33, 231, 352, 421, 1207, 1978, 2078
Bst4CI	ACNGT	6 cut(s)	157, 365, 1671, 1821, 2062, 2142
Bst6I	CTCTTC	2 cut(s)	1947, 2082
BstACI	GRCGYC	2 cut(s)	282, 356
BstAFI	CTTAAG	2 cut(s)	797, 1858
BstBAI	YACGTR	1 cut(s)	400
BstC8I	GCNNGC	7 cut(s)	314, 1089, 1212, 1511, 1747, 2030, 2034
BstDSI	CCRYGG	2 cut(s)	162, 471
BstEII	GGTNACC	1 cut(s)	1343
BstENI	CCTNNNNNAGG	1 cut(s)	975
BstF5I	GGATG	8 cut(s)	10, 273, 502, 1014, 1202, 1258, 1475, 1642
BstFNI	CGCG	5 cut(s)	164, 1525, 1812, 1938, 2203
BstH2I	RGCGCY	2 cut(s)	117, 1993
BstHHI	GCGC	6 cut(s)	31, 116, 1814, 1992, 2111, 2203
BstKTI	GATC	8 cut(s)	565, 760, 1156, 1498, 1692, 1867, 1906, 1968
BstMAI	GTCTC	7 cut(s)	108, 411, 601, 941, 1551, 1655, 1975
BstMBI	GATC	8 cut(s)	562, 757, 1153, 1495, 1689, 1864, 1903, 1965
BstNI	CCWGG	7 cut(s)	33, 231, 352, 421, 1207, 1978, 2078
BstNSI	RCATGY	2 cut(s)	2036, 2173
BstPI	GGTNACC	1 cut(s)	1343
BstSCI	CCNGG	7 cut(s)	31, 229, 350, 419, 1205, 1976, 2076
BstSFI	CTRYAG	2 cut(s)	69, 891
BstSLI	GKGCMC	1 cut(s)	1474
BstUI	CGCG	5 cut(s)	164, 1525, 1812, 1938, 2203
BstV2I	GAAGAC	2 cut(s)	519, 810
BstX2I	RGATCY	1 cut(s)	1689
BstYI	RGATCY	1 cut(s)	1689
Bsu15I	ATCGAT	1 cut(s)	1498
BsuI	GTATCC	1 cut(s)	30
BsuRI	GGCC	5 cut(s)	45, 229, 554, 1976, 2008
BsuTUI	ATCGAT	1 cut(s)	1498
BtgI	CCRYGG	2 cut(s)	162, 471
BtsCI	GGATG	8 cut(s)	10, 273, 502, 1014, 1202, 1258, 1475, 1642
BtsI	GCAGTG	2 cut(s)	988, 1795
BtsIMutI	CAGTG	2 cut(s)	988, 1795
Cac8I	GCNNGC	7 cut(s)	314, 1089, 1212, 1511, 1747, 2030, 2034
CaiI	CAGNNNCTG	2 cut(s)	68, 1309
CciI	TCATGA	1 cut(s)	1906
CfoI	GCGC	6 cut(s)	31, 116, 1814, 1992, 2111, 2203
Cfr10I	RCCGGY	1 cut(s)	2197
Cfr13I	GGNCC	2 cut(s)	251, 2007
Cfr42I	CCGCGG	1 cut(s)	165
ClaI	ATCGAT	1 cut(s)	1498
CseI	GACGC	2 cut(s)	290, 345
Csp6I	GTAC	4 cut(s)	87, 1565, 2114, 2195
CspCI	CAANNNNNGTGG	2 cut(s)	1581, 1616
CviQI	GTAC	4 cut(s)	87, 1565, 2114, 2195
DpnI	GATC	8 cut(s)	564, 759, 1155, 1497, 1691, 1866, 1905, 1967
DpnII	GATC	8 cut(s)	562, 757, 1153, 1495, 1689, 1864, 1903, 1965
DraIII	CACNNNGTG	1 cut(s)	1130
DrdI	GACNNNNNNGTC	2 cut(s)	284, 355
DseDI	GACNNNNNNGTC	2 cut(s)	284, 355
Eam1104I	CTCTTC	2 cut(s)	1947, 2082
EarI	CTCTTC	2 cut(s)	1947, 2082
EciI	GGCGGA	1 cut(s)	620
Ecl136II	GAGCTC	1 cut(s)	337
Eco24I	GRGCYC	3 cut(s)	95, 339, 1233
Eco31I	GGTCTC	3 cut(s)	411, 601, 941
Eco32I	GATATC	1 cut(s)	851
Eco47I	GGWCC	1 cut(s)	251
Eco53kI	GAGCTC	1 cut(s)	337
Eco57I	CTGAAG	3 cut(s)	702, 993, 1335
Eco72I	CACGTG	1 cut(s)	400
Eco91I	GGTNACC	1 cut(s)	1343
EcoICRI	GAGCTC	1 cut(s)	337
EcoNI	CCTNNNNNAGG	1 cut(s)	975
EcoO65I	GGTNACC	1 cut(s)	1343
EcoRI	GAATTC	1 cut(s)	1915
EcoRII	CCWGG	7 cut(s)	31, 229, 350, 419, 1205, 1976, 2076
EcoRV	GATATC	1 cut(s)	851
EcoT22I	ATGCAT	1 cut(s)	2175
EcoT38I	GRGCYC	3 cut(s)	95, 339, 1233
Esp3I	CGTCTC	1 cut(s)	108
FalI	AAGNNNNNCTT	2 cut(s)	166, 198
FaqI	GGGAC	2 cut(s)	272, 1260
FauI	CCCGC	2 cut(s)	262, 1931
FauNDI	CATATG	1 cut(s)	1876
FbaI	TGATCA	1 cut(s)	562
FblI	GTMKAC	1 cut(s)	2149
FokI	GGATG	8 cut(s)	17, 280, 509, 1021, 1189, 1265, 1462, 1629
FriOI	GRGCYC	3 cut(s)	95, 339, 1233
FspBI	CTAG	2 cut(s)	1199, 1427
GlaI	GCGC	6 cut(s)	30, 115, 1813, 1991, 2110, 2202
GsuI	CTGGAG	2 cut(s)	252, 323
HaeII	RGCGCY	2 cut(s)	117, 1993
HaeIII	GGCC	5 cut(s)	45, 229, 554, 1976, 2008
HapII	CCGG	3 cut(s)	192, 2198, 2242
HgaI	GACGC	2 cut(s)	290, 345
HhaI	GCGC	6 cut(s)	31, 116, 1814, 1992, 2111, 2203
Hin1I	GRCGYC	2 cut(s)	282, 356
Hin6I	GCGC	6 cut(s)	29, 114, 1812, 1990, 2109, 2201
HinP1I	GCGC	6 cut(s)	29, 114, 1812, 1990, 2109, 2201
HincII	GTYRAC	1 cut(s)	153
HindII	GTYRAC	1 cut(s)	153
HindIII	AAGCTT	2 cut(s)	1387, 1511
HinfI	GANTC	9 cut(s)	195, 503, 764, 775, 1046, 1170, 1193, 1639, 1696
HpaII	CCGG	3 cut(s)	192, 2198, 2242
HphI	GGTGA	6 cut(s)	121, 1116, 1319, 1355, 1505, 1830
Hpy166II	GTNNAC	4 cut(s)	153, 251, 1472, 2150
Hpy8I	GTNNAC	4 cut(s)	153, 251, 1472, 2150
Hpy99I	CGWCG	3 cut(s)	281, 284, 361
HpyAV	CCTTC	8 cut(s)	319, 586, 724, 971, 1484, 1585, 1880, 2049
HpyCH4III	ACNGT	6 cut(s)	157, 365, 1671, 1821, 2062, 2142
HpyCH4IV	ACGT	4 cut(s)	399, 572, 1547, 2132
HpySE526I	ACGT	4 cut(s)	399, 572, 1547, 2132
Hsp92I	GRCGYC	2 cut(s)	282, 356
HspAI	GCGC	6 cut(s)	29, 114, 1812, 1990, 2109, 2201
Kpn2I	TCCGGA	1 cut(s)	191
KpnI	GGTACC	1 cut(s)	2198
Ksp22I	TGATCA	1 cut(s)	562
KspI	CCGCGG	1 cut(s)	165
Kzo9I	GATC	8 cut(s)	562, 757, 1153, 1495, 1689, 1864, 1903, 1965
LguI	GCTCTTC	2 cut(s)	1947, 2082
LmnI	GCTCC	8 cut(s)	131, 233, 334, 342, 642, 832, 1812, 2164
LweI	GCATC	6 cut(s)	143, 808, 1211, 1296, 1783, 2238
MaeI	CTAG	2 cut(s)	1199, 1427
MaeII	ACGT	4 cut(s)	399, 572, 1547, 2132
MaeIII	GTNAC	4 cut(s)	157, 709, 1051, 1343
MalI	GATC	8 cut(s)	564, 759, 1155, 1497, 1691, 1866, 1905, 1967
MboI	GATC	8 cut(s)	562, 757, 1153, 1495, 1689, 1864, 1903, 1965
MfeI	CAATTG	1 cut(s)	529
MflI	RGATCY	1 cut(s)	1689
MhlI	GDGCHC	5 cut(s)	95, 339, 1233, 1474, 2169
MlyI	GAGTC	3 cut(s)	497, 773, 1040
MmeI	TCCRAC	2 cut(s)	78, 339
Mph1103I	ATGCAT	1 cut(s)	2175
MroI	TCCGGA	1 cut(s)	191
MroXI	GAANNNNTTC	1 cut(s)	2141
MseI	TTAA	8 cut(s)	798, 1002, 1031, 1106, 1400, 1859, 1925, 2096
MspA1I	CMGCKG	5 cut(s)	65, 164, 770, 1058, 1752
MspCI	CTTAAG	2 cut(s)	797, 1858
MspI	CCGG	3 cut(s)	192, 2198, 2242
MspR9I	CCNGG	7 cut(s)	33, 231, 352, 421, 1207, 1978, 2078
MunI	CAATTG	1 cut(s)	529
Mva1269I	GAATGC	3 cut(s)	657, 907, 1372
MvaI	CCWGG	7 cut(s)	33, 231, 352, 421, 1207, 1978, 2078
MvnI	CGCG	5 cut(s)	164, 1525, 1812, 1938, 2203
NdeI	CATATG	1 cut(s)	1876
NdeII	GATC	8 cut(s)	562, 757, 1153, 1495, 1689, 1864, 1903, 1965
NlaIV	GGNNCC	4 cut(s)	253, 1165, 1891, 2196
NmeAIII	GCCGAG	1 cut(s)	580
NmuCI	GTSAC	1 cut(s)	1343
NsiI	ATGCAT	1 cut(s)	2175
NspI	RCATGY	2 cut(s)	2036, 2173
PaeI	GCATGC	1 cut(s)	2036
PagI	TCATGA	1 cut(s)	1906
PciSI	GCTCTTC	2 cut(s)	1947, 2082
PcsI	WCGNNNNNNNCGW	1 cut(s)	282
PctI	GAATGC	3 cut(s)	657, 907, 1372
PdmI	GAANNNNTTC	1 cut(s)	2141
PfeI	GAWTC	6 cut(s)	195, 775, 1170, 1193, 1639, 1696
Pfl23II	CGTACG	1 cut(s)	86
PflFI	GACNNNGTC	1 cut(s)	284
PleI	GAGTC	3 cut(s)	497, 772, 1040
PmaCI	CACGTG	1 cut(s)	400
PmlI	CACGTG	1 cut(s)	400
PpsI	GAGTC	3 cut(s)	497, 772, 1040
Ppu21I	YACGTR	1 cut(s)	400
Psp124BI	GAGCTC	1 cut(s)	339
Psp1406I	AACGTT	1 cut(s)	2132
Psp6I	CCWGG	7 cut(s)	31, 229, 350, 419, 1205, 1976, 2076
PspCI	CACGTG	1 cut(s)	400
PspEI	GGTNACC	1 cut(s)	1343
PspGI	CCWGG	7 cut(s)	31, 229, 350, 419, 1205, 1976, 2076
PspLI	CGTACG	1 cut(s)	86
PspN4I	GGNNCC	4 cut(s)	253, 1165, 1891, 2196
PspPI	GGNCC	2 cut(s)	251, 2007
PstI	CTGCAG	2 cut(s)	73, 895
PstNI	CAGNNNCTG	2 cut(s)	68, 1309
PsuI	RGATCY	1 cut(s)	1689
PsyI	GACNNNGTC	1 cut(s)	284
PvuII	CAGCTG	2 cut(s)	770, 1058
RsaI	GTAC	4 cut(s)	88, 1566, 2115, 2196
RsaNI	GTAC	4 cut(s)	87, 1565, 2114, 2195
SacI	GAGCTC	1 cut(s)	339
SacII	CCGCGG	1 cut(s)	165
SapI	GCTCTTC	2 cut(s)	1947, 2082
SaqAI	TTAA	8 cut(s)	798, 1002, 1031, 1106, 1400, 1859, 1925, 2096
Sau3AI	GATC	8 cut(s)	562, 757, 1153, 1495, 1689, 1864, 1903, 1965
Sau96I	GGNCC	2 cut(s)	251, 2007
SchI	GAGTC	3 cut(s)	497, 773, 1040
ScrFI	CCNGG	7 cut(s)	33, 231, 352, 421, 1207, 1978, 2078
SduI	GDGCHC	5 cut(s)	95, 339, 1233, 1474, 2169
SfaNI	GCATC	6 cut(s)	143, 808, 1211, 1296, 1783, 2238
SfcI	CTRYAG	2 cut(s)	69, 891
Sfr303I	CCGCGG	1 cut(s)	165
SgrBI	CCGCGG	1 cut(s)	165
SinI	GGWCC	1 cut(s)	251
SmlI	CTYRAG	3 cut(s)	797, 1613, 1858
SmoI	CTYRAG	3 cut(s)	797, 1613, 1858
SphI	GCATGC	1 cut(s)	2036
SspMI	CTAG	2 cut(s)	1199, 1427
SstI	GAGCTC	1 cut(s)	339
StyD4I	CCNGG	7 cut(s)	31, 229, 350, 419, 1205, 1976, 2076
TaaI	ACNGT	6 cut(s)	157, 365, 1671, 1821, 2062, 2142
TaiI	ACGT	4 cut(s)	402, 575, 1550, 2135
TaqI	TCGA	6 cut(s)	78, 276, 778, 853, 1156, 1498
TaqII	GACCGA	2 cut(s)	965, 1857
TatI	WGTACW	1 cut(s)	2113
TauI	GCSGC	4 cut(s)	68, 167, 456, 1995
TfiI	GAWTC	6 cut(s)	195, 775, 1170, 1193, 1639, 1696
Tru1I	TTAA	8 cut(s)	798, 1002, 1031, 1106, 1400, 1859, 1925, 2096
Tru9I	TTAA	8 cut(s)	798, 1002, 1031, 1106, 1400, 1859, 1925, 2096
TscAI	CASTG	2 cut(s)	988, 1795
TseFI	GTSAC	1 cut(s)	1343
Tsp45I	GTSAC	1 cut(s)	1343
TspDTI	ATGAA	2 cut(s)	1025, 1110
TspGWI	ACGGA	1 cut(s)	90
TspRI	CASTG	2 cut(s)	988, 1795
Tth111I	GACNNNGTC	1 cut(s)	284
Vha464I	CTTAAG	2 cut(s)	797, 1858
VneI	GTGCAC	1 cut(s)	1470
VpaK11BI	GGWCC	1 cut(s)	251
XagI	CCTNNNNNAGG	1 cut(s)	975
XapI	RAATTY	3 cut(s)	1108, 1715, 1915
XceI	RCATGY	2 cut(s)	2036, 2173
XmiI	GTMKAC	1 cut(s)	2149
XmnI	GAANNNNTTC	1 cut(s)	2141
XspI	CTAG	2 cut(s)	1199, 1427
Zsp2I	ATGCAT	1 cut(s)	2175

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.