MD03G1296900.v1.1

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0009318
Physical Location & Seq
Reverse (-)
37280207 .. 37284563
4357 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1296900.v1.1.491

Sequence Viewer

Length: 2268 bp
ATGGATGGCCTTTGTCTGAAAACAACTGCGCCAGGGATACACGGCATATCTTCCACCATCTCAGCGGCTGCAGCCCTCGATATCCGTGCGAGCCCATCTCCATCTCAGGTGAGCGCCATTGGCCGCTCCTCTGCATCTGGGATTGACAAGTCCACAGCAACCGCGGTTCAGAAAAGGACGACGCCGTTTTCCAGATTCTCTTTAAAGCACCCTCTGAAATTCTTATGGCCTGGAGCAGCAAGAAGAGGTGGACCTGCCTACAATGGGATGGCTCTCGACGACGCCGTCCCCTTGCCCTCTAAGGAGGAAGAGGAGGCGGAGGCGGAGGCGGAGGAGGAGCGCCAGAGTGCGTCCAGGGCGTCGGACGGTCAAAGTGGGAACTGGGTTTTGAAGATTTTGCACGTGAGGTCTCTCTCTCTACCCAGGAGAGAGGAACAGCAGGGGGGCAAGATTGGCGGCGTTGCAGAGCTGCCCAGGGAAGATGGAAAAACCGTGATGGATGATGATGACAGAAATGGGAAGGCTGACTCGGAGGAAGACGATGACGAAAATGATTGTTGTAGTGTTTCAGAGGAGGCCGAGTTTGATAAAGATACGTTTAGTCGGTTGCTTAGGAAGGTTTCGTTGGCGGAGGCGAGGTTGTATGGTCAAATGTCGTACTTGGTGAACTTAGCTTATTCCATTCCTAAAATCCAGACAGGAAATCTCCTAAGAAGTTATGGTTTTCGTTTTGTTACTTCGTCAGTAGAGAAGAAGGAATTGGCTGCTAAAACCGAGGAGGACCAGGTGTCAGCTGAAATTCAAGAAACTGAAAAAAACCTTGAGGAAGACAGGGAAGAAGATGGGGAAATAAAGGAGCAAAAGAACAATGGATATCAAATAAGTCCATTTGCTGCTTATCAAATTGCTGCTTCTGCAGCTTCTTATCTGCATTCTCAAACAAGGAGCATACTTCCATTCAAATCCTCAGAGACTGAGGCAGATAAAGATCTCTCTGAAGAAGGCAGTGAAAGAAGCGAAGCTGTTAATAGGATGAACTCAGAAGTGGCTTCTTTAATGGCAACCACCGACTCAGTTACAGCCGTGGTTGCTGCAAAGGAAGAAGTAAAGCAGGCTGTTGCAGATGACTTAAATTCAACACTTTCATCACCTTGTGAATGGTTCGTATGCGATGATGATCAAAGCGGCACCAGATTCTTTGTCATTCAGGGGTCTGAATCACTAGCATCCTGGCAAGCAAATCTACTTTTTGAGCCCATTCAGTTTGAGGGACTGGATGTGCTTGTGCATAGAGGCATATACGAGGCTGCGAAAGGGATTTACAAACAGATGCTGCCTGGAGTCCATGCCCACCTCAAATCTCATGGTGACCATGCAACATTTCGTTTTACTGGGCATTCTCTAGGGGGAAGCTTGGCACTACTAATAAATCTCATGTTGCTTATACGGTTAGAAGTGCCAGTTTCTTCACTGCTTCCTGTTATTACTTTTGGTGCACCATCCATCATGTGTGGAGGTTATCAATTGCTTCGCAAGCTTGGATTACCACAAAGTCATGTTCAGTCTATTATGTTGCACAGAGACATTGTACCCCGTGCCTTCTCTTGCAATTACCCAAATCATGTGGCAGAGCTTCTCAAGGCTTTGAATGGGAACTTTCGGAATCATCCCTGTCTCAATAACCAGAAGCTACTGTATTCTCCAATGGGGGAGCTCCTGATTATTCAACCAGATGAGAAATTCTCCCCAAATCATCATCTTCTTCCATCAGGCAGTGGTCTATATCTTCTAAGCTGCCCACTGTCTGATGGCAGTGATGCAGAGAGGCAGCTCCGGGCTGCACAGTTGGTATTCCTAAACTCACCACACCCACTCGAGATCCTAAGTGACCGGTCTGCATATGGTTCAGAAGGAACCATTCAAAGAGATCATGATGTGAATTCTTATTTAAAATCTGTTCGTGGGGTAATTCGGCAAGAGCTTAACCAGATTAGGAAGTCTAGGAGACAACAGCGCCGCAAGGTGTGGTGGCCCTTGATAGCACCCCGGAGCGTGCATGCAGGAGGTGTGATTGTGGGAAGGACTGTTGCCTCAATCAACCTGAGGCGAGAGCAATTAAACTTTTCTGGCGTATTACGGACGGGGAGAGAGTCGTTGAAACGGTTCGGTAGAATTGTTGCATCGTGGCACATGCATTTGTTTGTGGTGCTTTTGGTACCAGCGCGATTGTTAGTCTTGGGGGCATACAGCATCATCAGTTTGCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

756

Amino Acids

83.2

Weight (kDa)

6.13

Isoelectric Point (pI)

49.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 400 - 537 3.8e-25 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 284
Acc36I ACCTGC 1 cut(s) 262
Acc65I GGTACC 1 cut(s) 2215
AccB1I GGYRCC 2 cut(s) 1187, 2215
AccBSI CCGCTC 1 cut(s) 126
AccII CGCG 2 cut(s) 164, 2224
AclWI GGATC 1 cut(s) 1873
AcoI YGGCCR 1 cut(s) 121
AcsI RAATTY 5 cut(s) 218, 798, 1132, 1739, 1939
AcuI CTGAAG 1 cut(s) 1017
AcvI CACGTG 1 cut(s) 403
AcyI GRCGYC 3 cut(s) 182, 282, 359
AdeI CACNNNGTG 1 cut(s) 1154
AfaI GTAC 3 cut(s) 659, 1590, 2217
AfiI CCNNNNNNNGG 1 cut(s) 264
AgeI ACCGGT 1 cut(s) 1890
AgsI TTSAA 8 cut(s) 391, 803, 961, 1137, 1648, 1727, 1922, 2158
AhdI GACNNNNNGTC 1 cut(s) 787
AjnI CCWGG 8 cut(s) 31, 229, 353, 422, 473, 783, 1229, 1336
AjuI GAANNNNNNNTTGG 4 cut(s) 608, 640, 743, 775
Alw21I GWGCWC 2 cut(s) 1498, 1716
Alw26I GTCTC 5 cut(s) 414, 965, 1575, 1679, 1999
Alw44I GTGCAC 1 cut(s) 1494
AlwI GGATC 1 cut(s) 1873
AlwNI CAGNNNCTG 3 cut(s) 68, 974, 1333
Ama87I CYCGRG 1 cut(s) 1874
AoxI GGCC 5 cut(s) 7, 121, 227, 576, 2030
ApaLI GTGCAC 1 cut(s) 1494
ApoI RAATTY 5 cut(s) 218, 798, 1132, 1739, 1939
AsiGI ACCGGT 1 cut(s) 1890
Asp700I GAANNNNTTC 1 cut(s) 2162
Asp718I GGTACC 1 cut(s) 2215
AspLEI GCGC 5 cut(s) 31, 116, 342, 2016, 2224
AspS9I GGNCC 3 cut(s) 251, 781, 2031
AsuC2I CCSGG 2 cut(s) 1835, 2047
AsuHPI GGTGA 5 cut(s) 121, 676, 1140, 1379, 1854
AvaI CYCGRG 1 cut(s) 1874
AvaII GGWCC 2 cut(s) 251, 781
AxyI CCTNAGG 1 cut(s) 2102
BaeGI GKGCMC 1 cut(s) 1498
BanI GGYRCC 2 cut(s) 1187, 2215
BanII GRGCYC 3 cut(s) 95, 1257, 1716
BbrPI CACGTG 1 cut(s) 403
BbsI GAAGAC 2 cut(s) 543, 834
Bbv12I GWGCWC 2 cut(s) 1498, 1716
BceAI ACGGC 4 cut(s) 58, 169, 269, 1067
BcgI CGANNNNNNTGC 2 cut(s) 68, 102
BciT130I CCWGG 8 cut(s) 33, 231, 355, 424, 475, 785, 1231, 1338
BciVI GTATCC 1 cut(s) 30
BclI TGATCA 1 cut(s) 1177
BcnI CCSGG 2 cut(s) 1835, 2047
BcoDI GTCTC 5 cut(s) 414, 965, 1575, 1679, 1999
BfaI CTAG 3 cut(s) 1223, 1403, 2001
BfmI CTRYAG 2 cut(s) 69, 915
BfoI RGCGCY 3 cut(s) 117, 343, 2017
BfuAI ACCTGC 1 cut(s) 262
BfuI GTATCC 1 cut(s) 30
BglII AGATCT 1 cut(s) 988
Bme18I GGWCC 2 cut(s) 251, 781
BmeRI GACNNNNNGTC 1 cut(s) 787
BmeT110I CYCGRG 1 cut(s) 1874
BmgT120I GGNCC 3 cut(s) 251, 781, 2031
BmiI GGNNCC 3 cut(s) 1189, 1915, 2217
BmrI ACTGGG 2 cut(s) 391, 1401
BmsI GCATC 6 cut(s) 143, 1235, 1320, 1807, 2189, 2259
BmuI ACTGGG 2 cut(s) 391, 1401
BpiI GAAGAC 2 cut(s) 543, 834
BplI GAGNNNNNCTC 4 cut(s) 82, 114, 1727, 1759
BpmI CTGGAG 2 cut(s) 252, 1359
Bpu10I CCTNAGC 1 cut(s) 611
BpuEI CTTGAG 2 cut(s) 842, 1622
BpuMI CCSGG 2 cut(s) 1835, 2047
BsaAI YACGTR 1 cut(s) 403
BsaBI GATNNNNATC 2 cut(s) 987, 1176
BsaHI GRCGYC 3 cut(s) 182, 282, 359
BsaI GGTCTC 1 cut(s) 414
BsaJI CCNNGG 9 cut(s) 32, 162, 354, 422, 473, 474, 774, 1083, 2045
BsaWI WCCGGW 1 cut(s) 1890
BsaXI ACNNNNNCTCC 4 cut(s) 566, 596, 623, 653
Bsc4I CCNNNNNNNGG 1 cut(s) 264
Bse118I RCCGGY 1 cut(s) 1890
Bse1I ACTGG 4 cut(s) 386, 1278, 1396, 1460
Bse21I CCTNAGG 1 cut(s) 2102
Bse8I GATNNNNATC 2 cut(s) 987, 1176
BseBI CCWGG 8 cut(s) 33, 231, 355, 424, 475, 785, 1231, 1338
BseDI CCNNGG 9 cut(s) 32, 162, 354, 422, 473, 474, 774, 1083, 2045
BseGI GGATG 8 cut(s) 10, 273, 505, 1038, 1226, 1282, 1499, 1666
BseJI GATNNNNATC 2 cut(s) 987, 1176
BseLI CCNNNNNNNGG 1 cut(s) 264
BseMII CTCAG 7 cut(s) 75, 119, 966, 981, 1053, 1086, 2093
BseNI ACTGG 4 cut(s) 386, 1278, 1396, 1460
BseRI GAGGAG 6 cut(s) 118, 326, 347, 350, 587, 791
BseSI GKGCMC 1 cut(s) 1498
BsgI GTGCAG 1 cut(s) 1824
Bsh1236I CGCG 2 cut(s) 164, 2224
BshFI GGCC 5 cut(s) 9, 123, 229, 578, 2032
BshNI GGYRCC 2 cut(s) 1187, 2215
BshTI ACCGGT 1 cut(s) 1890
BsiHKAI GWGCWC 2 cut(s) 1498, 1716
BsiHKCI CYCGRG 1 cut(s) 1874
BsiSI CCGG 3 cut(s) 1834, 1891, 2047
BslFI GGGAC 2 cut(s) 272, 1284
BslI CCNNNNNNNGG 1 cut(s) 264
BsmAI GTCTC 5 cut(s) 414, 965, 1575, 1679, 1999
BsmFI GGGAC 2 cut(s) 272, 1284
BsmI GAATGC 2 cut(s) 931, 1396
BsnI GGCC 5 cut(s) 9, 123, 229, 578, 2032
Bso31I GGTCTC 1 cut(s) 414
BsoBI CYCGRG 1 cut(s) 1874
Bsp1286I GDGCHC 4 cut(s) 95, 1257, 1498, 1716
Bsp143I GATC 4 cut(s) 988, 1177, 1878, 1927
BspANI GGCC 5 cut(s) 9, 123, 229, 578, 2032
BspCNI CTCAG 7 cut(s) 74, 118, 967, 980, 1052, 1085, 2094
BspFNI CGCG 2 cut(s) 164, 2224
BspHI TCATGA 1 cut(s) 1930
BspLI GGNNCC 3 cut(s) 1189, 1915, 2217
BspMAI CTGCAG 2 cut(s) 73, 919
BspMI ACCTGC 1 cut(s) 262
BspPI GGATC 1 cut(s) 1873
BspT107I GGYRCC 2 cut(s) 1187, 2215
BspTNI GGTCTC 1 cut(s) 414
BsrBI CCGCTC 1 cut(s) 126
BsrFI RCCGGY 1 cut(s) 1890
BsrI ACTGG 4 cut(s) 386, 1278, 1396, 1460
BssAI RCCGGY 1 cut(s) 1890
BssECI CCNNGG 9 cut(s) 32, 162, 354, 422, 473, 474, 774, 1083, 2045
BssMI GATC 4 cut(s) 988, 1177, 1878, 1927
BssNI GRCGYC 3 cut(s) 182, 282, 359
Bst2UI CCWGG 8 cut(s) 33, 231, 355, 424, 475, 785, 1231, 1338
Bst4CI ACNGT 8 cut(s) 368, 493, 1449, 1695, 1803, 1845, 2086, 2163
Bst6I CTCTTC 2 cut(s) 238, 303
BstACI GRCGYC 3 cut(s) 182, 282, 359
BstBAI YACGTR 1 cut(s) 403
BstC8I GCNNGC 6 cut(s) 91, 1113, 1236, 1535, 2054, 2058
BstDSI CCRYGG 2 cut(s) 162, 1083
BstEII GGTNACC 1 cut(s) 1367
BstF5I GGATG 8 cut(s) 10, 273, 505, 1038, 1226, 1282, 1499, 1666
BstFNI CGCG 2 cut(s) 164, 2224
BstH2I RGCGCY 3 cut(s) 117, 343, 2017
BstHHI GCGC 5 cut(s) 31, 116, 342, 2016, 2224
BstKTI GATC 4 cut(s) 991, 1180, 1881, 1930
BstMAI GTCTC 5 cut(s) 414, 965, 1575, 1679, 1999
BstMBI GATC 4 cut(s) 988, 1177, 1878, 1927
BstMWI GCNNNNNNNGC 8 cut(s) 71, 120, 356, 453, 914, 917, 1088, 1534
BstNI CCWGG 8 cut(s) 33, 231, 355, 424, 475, 785, 1231, 1338
BstNSI RCATGY 2 cut(s) 2060, 2194
BstPI GGTNACC 1 cut(s) 1367
BstSFI CTRYAG 2 cut(s) 69, 915
BstSLI GKGCMC 1 cut(s) 1498
BstUI CGCG 2 cut(s) 164, 2224
BstV2I GAAGAC 2 cut(s) 543, 834
BstX2I RGATCY 2 cut(s) 988, 1878
BstYI RGATCY 2 cut(s) 988, 1878
Bsu36I CCTNAGG 1 cut(s) 2102
BsuI GTATCC 1 cut(s) 30
BsuRI GGCC 5 cut(s) 9, 123, 229, 578, 2032
BtgI CCRYGG 2 cut(s) 162, 1083
BtgZI GCGATG 1 cut(s) 1185
BtsCI GGATG 8 cut(s) 10, 273, 505, 1038, 1226, 1282, 1499, 1666
BtsI GCAGTG 4 cut(s) 1012, 1469, 1780, 1819
BtsIMutI CAGTG 5 cut(s) 1012, 1469, 1780, 1799, 1819
BveI ACCTGC 1 cut(s) 262
Cac8I GCNNGC 6 cut(s) 91, 1113, 1236, 1535, 2054, 2058
CaiI CAGNNNCTG 3 cut(s) 68, 974, 1333
CciI TCATGA 1 cut(s) 1930
CfoI GCGC 5 cut(s) 31, 116, 342, 2016, 2224
Cfr10I RCCGGY 1 cut(s) 1890
Cfr13I GGNCC 3 cut(s) 251, 781, 2031
Cfr42I CCGCGG 1 cut(s) 165
CseI GACGC 4 cut(s) 190, 290, 339, 348
CsiI ACCWGGT 1 cut(s) 783
Csp6I GTAC 3 cut(s) 658, 1589, 2216
CspAI ACCGGT 1 cut(s) 1890
CspCI CAANNNNNGTGG 2 cut(s) 1605, 1640
CviQI GTAC 3 cut(s) 658, 1589, 2216
DpnI GATC 4 cut(s) 990, 1179, 1880, 1929
DpnII GATC 4 cut(s) 988, 1177, 1878, 1927
DraI TTTAAA 2 cut(s) 204, 1950
DraIII CACNNNGTG 1 cut(s) 1154
DrdI GACNNNNNNGTC 1 cut(s) 284
DriI GACNNNNNGTC 1 cut(s) 787
DseDI GACNNNNNNGTC 1 cut(s) 284
EaeI YGGCCR 1 cut(s) 121
Eam1104I CTCTTC 2 cut(s) 238, 303
Eam1105I GACNNNNNGTC 1 cut(s) 787
EarI CTCTTC 2 cut(s) 238, 303
EciI GGCGGA 4 cut(s) 332, 338, 344, 644
Ecl136II GAGCTC 1 cut(s) 1714
Eco24I GRGCYC 3 cut(s) 95, 1257, 1716
Eco31I GGTCTC 1 cut(s) 414
Eco32I GATATC 2 cut(s) 82, 875
Eco47I GGWCC 2 cut(s) 251, 781
Eco53kI GAGCTC 1 cut(s) 1714
Eco57I CTGAAG 1 cut(s) 1017
Eco72I CACGTG 1 cut(s) 403
Eco81I CCTNAGG 1 cut(s) 2102
Eco88I CYCGRG 1 cut(s) 1874
Eco91I GGTNACC 1 cut(s) 1367
EcoICRI GAGCTC 1 cut(s) 1714
EcoO65I GGTNACC 1 cut(s) 1367
EcoRI GAATTC 1 cut(s) 1939
EcoRII CCWGG 8 cut(s) 31, 229, 353, 422, 473, 783, 1229, 1336
EcoRV GATATC 2 cut(s) 82, 875
EcoT22I ATGCAT 1 cut(s) 2196
EcoT38I GRGCYC 3 cut(s) 95, 1257, 1716
FaqI GGGAC 2 cut(s) 272, 1284
FauNDI CATATG 1 cut(s) 1900
FbaI TGATCA 1 cut(s) 1177
FokI GGATG 8 cut(s) 17, 280, 512, 1045, 1213, 1289, 1486, 1653
FriOI GRGCYC 3 cut(s) 95, 1257, 1716
FspBI CTAG 3 cut(s) 1223, 1403, 2001
GlaI GCGC 5 cut(s) 30, 115, 341, 2015, 2223
GsuI CTGGAG 2 cut(s) 252, 1359
HaeII RGCGCY 3 cut(s) 117, 343, 2017
HaeIII GGCC 5 cut(s) 9, 123, 229, 578, 2032
HapII CCGG 3 cut(s) 1834, 1891, 2047
HgaI GACGC 4 cut(s) 190, 290, 339, 348
HhaI GCGC 5 cut(s) 31, 116, 342, 2016, 2224
Hin1I GRCGYC 3 cut(s) 182, 282, 359
Hin6I GCGC 5 cut(s) 29, 114, 340, 2014, 2222
HinP1I GCGC 5 cut(s) 29, 114, 340, 2014, 2222
HindIII AAGCTT 2 cut(s) 1411, 1535
HinfI GANTC 8 cut(s) 195, 527, 1070, 1194, 1217, 1341, 1663, 2150
HpaII CCGG 3 cut(s) 1834, 1891, 2047
HphI GGTGA 5 cut(s) 121, 676, 1140, 1379, 1854
Hpy166II GTNNAC 4 cut(s) 153, 251, 667, 1496
Hpy188III TCNNGA 7 cut(s) 192, 275, 694, 803, 1717, 1876, 1931
Hpy8I GTNNAC 4 cut(s) 153, 251, 667, 1496
Hpy99I CGWCG 4 cut(s) 184, 281, 284, 364
HpyAV CCTTC 7 cut(s) 514, 610, 748, 995, 1609, 1904, 2073
HpyCH4III ACNGT 8 cut(s) 368, 493, 1449, 1695, 1803, 1845, 2086, 2163
HpyCH4IV ACGT 2 cut(s) 402, 596
HpyF10VI GCNNNNNNNGC 8 cut(s) 71, 120, 356, 453, 914, 917, 1088, 1534
HpySE526I ACGT 2 cut(s) 402, 596
Hsp92I GRCGYC 3 cut(s) 182, 282, 359
HspAI GCGC 5 cut(s) 29, 114, 340, 2014, 2222
KpnI GGTACC 1 cut(s) 2219
Ksp22I TGATCA 1 cut(s) 1177
KspI CCGCGG 1 cut(s) 165
Kzo9I GATC 4 cut(s) 988, 1177, 1878, 1927
LmnI GCTCC 9 cut(s) 131, 233, 337, 856, 945, 1711, 1719, 1836, 2049
LweI GCATC 6 cut(s) 143, 1235, 1320, 1807, 2189, 2259
MabI ACCWGGT 1 cut(s) 783
MaeI CTAG 3 cut(s) 1223, 1403, 2001
MaeII ACGT 2 cut(s) 402, 596
MaeIII GTNAC 4 cut(s) 733, 1075, 1367, 1886
MalI GATC 4 cut(s) 990, 1179, 1880, 1929
MbiI CCGCTC 1 cut(s) 126
MboI GATC 4 cut(s) 988, 1177, 1878, 1927
MfeI CAATTG 1 cut(s) 1523
MflI RGATCY 2 cut(s) 988, 1878
MhlI GDGCHC 4 cut(s) 95, 1257, 1498, 1716
MlyI GAGTC 4 cut(s) 521, 1064, 1350, 2159
MmeI TCCRAC 1 cut(s) 342
Mph1103I ATGCAT 1 cut(s) 2196
MroXI GAANNNNTTC 1 cut(s) 2162
MseI TTAA 7 cut(s) 203, 1026, 1055, 1130, 1949, 1983, 2117
MspA1I CMGCKG 3 cut(s) 65, 164, 794
MspI CCGG 3 cut(s) 1834, 1891, 2047
MunI CAATTG 1 cut(s) 1523
Mva1269I GAATGC 2 cut(s) 931, 1396
MvaI CCWGG 8 cut(s) 33, 231, 355, 424, 475, 785, 1231, 1338
MvnI CGCG 2 cut(s) 164, 2224
MwoI GCNNNNNNNGC 8 cut(s) 71, 120, 356, 453, 914, 917, 1088, 1534
NciI CCSGG 2 cut(s) 1835, 2047
NdeI CATATG 1 cut(s) 1900
NdeII GATC 4 cut(s) 988, 1177, 1878, 1927
NlaIV GGNNCC 3 cut(s) 1189, 1915, 2217
NmeAIII GCCGAG 1 cut(s) 604
NmuCI GTSAC 2 cut(s) 1367, 1886
NsiI ATGCAT 1 cut(s) 2196
NspI RCATGY 2 cut(s) 2060, 2194
PaeI GCATGC 1 cut(s) 2060
PaeR7I CTCGAG 1 cut(s) 1874
PagI TCATGA 1 cut(s) 1930
PasI CCCWGGG 1 cut(s) 474
PcsI WCGNNNNNNNCGW 1 cut(s) 282
PctI GAATGC 2 cut(s) 931, 1396
PdmI GAANNNNTTC 1 cut(s) 2162
PfeI GAWTC 4 cut(s) 195, 1194, 1217, 1663
PflFI GACNNNGTC 1 cut(s) 284
PinAI ACCGGT 1 cut(s) 1890
PleI GAGTC 4 cut(s) 521, 1064, 1349, 2158
PmaCI CACGTG 1 cut(s) 403
PmlI CACGTG 1 cut(s) 403
PpsI GAGTC 4 cut(s) 521, 1064, 1349, 2158
Ppu21I YACGTR 1 cut(s) 403
Psp124BI GAGCTC 1 cut(s) 1716
Psp6I CCWGG 8 cut(s) 31, 229, 353, 422, 473, 783, 1229, 1336
PspCI CACGTG 1 cut(s) 403
PspEI GGTNACC 1 cut(s) 1367
PspGI CCWGG 8 cut(s) 31, 229, 353, 422, 473, 783, 1229, 1336
PspN4I GGNNCC 3 cut(s) 1189, 1915, 2217
PspPI GGNCC 3 cut(s) 251, 781, 2031
PstI CTGCAG 2 cut(s) 73, 919
PstNI CAGNNNCTG 3 cut(s) 68, 974, 1333
PsuI RGATCY 2 cut(s) 988, 1878
PsyI GACNNNGTC 1 cut(s) 284
PvuII CAGCTG 1 cut(s) 794
RsaI GTAC 3 cut(s) 659, 1590, 2217
RsaNI GTAC 3 cut(s) 658, 1589, 2216
SacI GAGCTC 1 cut(s) 1716
SacII CCGCGG 1 cut(s) 165
SaqAI TTAA 7 cut(s) 203, 1026, 1055, 1130, 1949, 1983, 2117
Sau3AI GATC 4 cut(s) 988, 1177, 1878, 1927
Sau96I GGNCC 3 cut(s) 251, 781, 2031
SchI GAGTC 4 cut(s) 521, 1064, 1350, 2159
SduI GDGCHC 4 cut(s) 95, 1257, 1498, 1716
SexAI ACCWGGT 1 cut(s) 783
SfaNI GCATC 6 cut(s) 143, 1235, 1320, 1807, 2189, 2259
SfcI CTRYAG 2 cut(s) 69, 915
Sfr274I CTCGAG 1 cut(s) 1874
Sfr303I CCGCGG 1 cut(s) 165
SgrBI CCGCGG 1 cut(s) 165
SinI GGWCC 2 cut(s) 251, 781
SlaI CTCGAG 1 cut(s) 1874
SmlI CTYRAG 3 cut(s) 821, 1637, 1874
SmoI CTYRAG 3 cut(s) 821, 1637, 1874
SphI GCATGC 1 cut(s) 2060
SspMI CTAG 3 cut(s) 1223, 1403, 2001
SstI GAGCTC 1 cut(s) 1716
TaaI ACNGT 8 cut(s) 368, 493, 1449, 1695, 1803, 1845, 2086, 2163
TaiI ACGT 2 cut(s) 405, 599
TaqI TCGA 3 cut(s) 78, 276, 1875
TauI GCSGC 5 cut(s) 68, 126, 459, 1188, 2019
TfiI GAWTC 4 cut(s) 195, 1194, 1217, 1663
Tru1I TTAA 7 cut(s) 203, 1026, 1055, 1130, 1949, 1983, 2117
Tru9I TTAA 7 cut(s) 203, 1026, 1055, 1130, 1949, 1983, 2117
TscAI CASTG 5 cut(s) 1012, 1476, 1780, 1806, 1819
TseFI GTSAC 2 cut(s) 1367, 1886
Tsp45I GTSAC 2 cut(s) 1367, 1886
TspDTI ATGAA 2 cut(s) 1049, 1134
TspGWI ACGGA 2 cut(s) 74, 2152
TspRI CASTG 5 cut(s) 1012, 1476, 1780, 1806, 1819
Tth111I GACNNNGTC 1 cut(s) 284
VneI GTGCAC 1 cut(s) 1494
VpaK11BI GGWCC 2 cut(s) 251, 781
XapI RAATTY 5 cut(s) 218, 798, 1132, 1739, 1939
XceI RCATGY 2 cut(s) 2060, 2194
XhoI CTCGAG 1 cut(s) 1874
XmnI GAANNNNTTC 1 cut(s) 2162
XspI CTAG 3 cut(s) 1223, 1403, 2001
Zsp2I ATGCAT 1 cut(s) 2196
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.