Rh7DG486900

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0009318
Physical Location & Seq
Reverse (-)
69224506 .. 69230226
5721 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG486900.1

Sequence Viewer

Length: 1662 bp
ATGGATAATTTTTGTTTGAAAACCACAACGGGGATACCCGGAATAGCTCCGACAATTACAGCCGCCCTGCCGATCGACGTGCGTACGAACCCGTCTCAGGTGAGCGCGCTGGGGAGGTCCACCGTGGACAACAATCACAAGAGGTTCTCCTTCAAGTACCCTTTGAAATCTCTCTGGCCCGGCGCCGGAAGAGGAGCCGCTCCTGCGCACAATGGGTTGCCTCTCGACGACGCCGTTCCTTTGGCCCAGGAGGAGGAGGACGACTGCCTTACCCCAACCCAAACCCAAAGTGGCAACTGGGTTTTAAAGATTTTGCACGTCAGGTCTCTCTGGGGAGGAGGAGGAGGAGGAGCAGATCAACAGGGTAAGGCGGAGCTGCGTGGGGAAGATGAAGCCGCGCCCAACGATAACAGAAATGGTGATGATGACGAAAATGACGACGATTGTGATGGTTGTAGAATTGAACAACAAGACGATCATCAACAAGAGCAGCAGCAGGTTGAGTTTGATAGAGATTCGTTTTCCAGATTGCTTCGGAAGGTTTCCTTGGCGGAAACCAGGTTATATGCTCAGATGTCGTATTTGGGGAGCTTGGCTTACTCCATTCCCAAAATCCAGCCACAAAATCTCTTGAGAGGTCATGCTCTGCGGTTTGTTACTTCTTCAATAGAAAAGAAGGAACTTGCTGCTAAAATTGAGAAACATCAAGAGTCGGCTGCAGTTAAAGAAACTGAAAGCACCCTAAAGGAAGACATGGAAGAAGACGAGGAAGGGAAGGAGCAGAAGAACAATGGATACAGAATAAGTCCCTCTGCTGCTTATCATATTGCTGCCTCTGCAGCTTCCTATTTGCATTCCCATACAAGGAGCATACTTCCATTCAAATCCTCAAAGGATGAGACTGATCAAGATTCAACAGAAGAAAGCAGAGGAAGAGGCAGCGCTGTTAATATGATGAACTCAGACATGGCTTCTTTGATGGCAACCACGGACTCAGTAACTTCTGTGGTTGCTGCAAAGGAAGAAGTAAAGCAGGCTGTTGCAGATGATTTAAACTCAACACATTCATCCCCGTGCGACTGGTTCATATGTGATGATGATCAAAGTGCCACCAGATTCTTGGTCATTCAGGGGTCTGAATCTCTGGCATCCTGGCAAGCAAATCTACTTTTCGAGCCCATTCAATTCGAGGGACTGGATGTGCTTGTGCATAGAGGCATATATGAGGCTGCAAAAGGGATTTATGGACAGGTGTTGCCTGAAGTTCATGCACACCTCAAATCTCATGGTGATAAAGCAACCTTCCGTTTTACTGGGCACTCATTAGGGGGAAGCTTAGCACTACTAATAAACCTCATGCTGCTTATACGGGGTGAAGTGCCAGTTTCTTCATTACTTCCTGTTATAACTTTTGGTTCACCATCCATCATGTGTGGAGGTGATCAACTGCTTCGCAAACTTGGATTACCACAGAGTCATATCCAGGCAATTACATTGCACAGAGACATTGTACCCCGGGCATTCTCTTGCAATTATCCTAATCATGTGGCAGAGCTTCTCAAAGCTTTAAGTGGGAACTTCCGGAATCATCCCTGCCTTAATAACCAGGTAAACCTTTTAGCTTTTGAGAAAAAGCATCAGTCTGAGATTATGAAACACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

553

Amino Acids

60.7

Weight (kDa)

5.45

Isoelectric Point (pI)

46.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 374 - 511 1.1e-26 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1406
Acc16I TGCGCA 1 cut(s) 207
Acc36I ACCTGC 1 cut(s) 487
AccB1I GGYRCC 1 cut(s) 182
AccBSI CCGCTC 1 cut(s) 200
AccII CGCG 2 cut(s) 107, 398
AccIII TCCGGA 1 cut(s) 1581
AciI CCGC 6 cut(s) 63, 198, 371, 396, 551, 649
AcuI CTGAAG 1 cut(s) 1281
AcyI GRCGYC 2 cut(s) 183, 231
AfaI GTAC 3 cut(s) 85, 158, 1512
AfeI AGCGCT 1 cut(s) 943
AfiI CCNNNNNNNGG 5 cut(s) 30, 97, 185, 253, 864
AgsI TTSAA 8 cut(s) 19, 154, 166, 464, 666, 883, 915, 1184
AjiI CACGTC 2 cut(s) 79, 319
AjnI CCWGG 5 cut(s) 246, 557, 1151, 1482, 1605
AjuI GAANNNNNNNTTGG 2 cut(s) 530, 562
AluBI AGCT 8 cut(s) 47, 376, 591, 842, 1335, 1555, 1565, 1622
AluI AGCT 8 cut(s) 47, 376, 591, 842, 1335, 1555, 1565, 1622
Alw26I GTCTC 4 cut(s) 99, 330, 893, 1497
Ama87I CYCGRG 1 cut(s) 1515
Aor13HI TCCGGA 1 cut(s) 1581
Aor51HI AGCGCT 1 cut(s) 943
AoxI GGCC 2 cut(s) 176, 243
AspLEI GCGC 6 cut(s) 107, 109, 185, 208, 400, 944
AspS9I GGNCC 3 cut(s) 117, 177, 244
AsuC2I CCSGG 4 cut(s) 39, 180, 1516, 1517
AsuHPI GGTGA 6 cut(s) 112, 431, 1301, 1385, 1410, 1451
AvaI CYCGRG 1 cut(s) 1515
AvaII GGWCC 1 cut(s) 117
BaeGI GKGCMC 1 cut(s) 1320
BanI GGYRCC 1 cut(s) 182
BanII GRGCYC 1 cut(s) 1179
BbsI GAAGAC 2 cut(s) 756, 768
BccI CCATC 4 cut(s) 443, 973, 1429, 1433
BceAI ACGGC 1 cut(s) 218
BcgI CGANNNNNNTGC 2 cut(s) 61, 95
BciT130I CCWGG 5 cut(s) 248, 559, 1153, 1484, 1607
BciVI GTATCC 2 cut(s) 27, 788
BclI TGATCA 3 cut(s) 904, 1099, 1441
BcnI CCSGG 4 cut(s) 39, 180, 1516, 1517
BcoDI GTCTC 4 cut(s) 99, 330, 893, 1497
BfmI CTRYAG 2 cut(s) 717, 837
BfoI RGCGCY 2 cut(s) 186, 945
BfuAI ACCTGC 1 cut(s) 487
BfuI GTATCC 2 cut(s) 27, 788
BlpI GCTNAGC 1 cut(s) 1336
Bme1390I CCNGG 9 cut(s) 39, 180, 248, 559, 1153, 1484, 1516, 1517, 1607
Bme18I GGWCC 1 cut(s) 117
BmeT110I CYCGRG 1 cut(s) 1515
BmgBI CACGTC 2 cut(s) 79, 319
BmgT120I GGNCC 3 cut(s) 117, 177, 244
BmiI GGNNCC 2 cut(s) 184, 196
BmrFI CCNGG 9 cut(s) 39, 180, 248, 559, 1153, 1484, 1516, 1517, 1607
BmrI ACTGGG 2 cut(s) 307, 1323
BmsI GCATC 2 cut(s) 1157, 1645
BmuI ACTGGG 2 cut(s) 307, 1323
BpiI GAAGAC 2 cut(s) 756, 768
Bpu1102I GCTNAGC 1 cut(s) 1336
BpuEI CTTGAG 1 cut(s) 652
BpuMI CCSGG 4 cut(s) 39, 180, 1516, 1517
BsaBI GATNNNNATC 1 cut(s) 1098
BsaHI GRCGYC 2 cut(s) 183, 231
BsaI GGTCTC 1 cut(s) 330
BsaJI CCNNGG 6 cut(s) 123, 246, 546, 987, 1514, 1515
BsaWI WCCGGW 1 cut(s) 1581
Bsc4I CCNNNNNNNGG 5 cut(s) 30, 97, 185, 253, 864
Bse1I ACTGG 5 cut(s) 302, 1085, 1200, 1318, 1382
Bse3DI GCAATG 1 cut(s) 1493
Bse8I GATNNNNATC 1 cut(s) 1098
BseAI TCCGGA 1 cut(s) 1581
BseBI CCWGG 5 cut(s) 248, 559, 1153, 1484, 1607
BseDI CCNNGG 6 cut(s) 123, 246, 546, 987, 1514, 1515
BseGI GGATG 6 cut(s) 901, 1067, 1148, 1204, 1421, 1588
BseJI GATNNNNATC 1 cut(s) 1098
BseLI CCNNNNNNNGG 5 cut(s) 30, 97, 185, 253, 864
BseMI GCAATG 1 cut(s) 1493
BseMII CTCAG 5 cut(s) 110, 584, 975, 1008, 1635
BseNI ACTGG 5 cut(s) 302, 1085, 1200, 1318, 1382
BsePI GCGCGC 1 cut(s) 105
BseRI GAGGAG 8 cut(s) 207, 266, 269, 351, 354, 357, 360, 363
BseSI GKGCMC 1 cut(s) 1320
BseYI CCCAGC 1 cut(s) 109
Bsh1236I CGCG 2 cut(s) 107, 398
Bsh1285I CGRYCG 1 cut(s) 75
BshFI GGCC 2 cut(s) 178, 245
BshNI GGYRCC 1 cut(s) 182
BsiEI CGRYCG 1 cut(s) 75
BsiHKCI CYCGRG 1 cut(s) 1515
BsiSI CCGG 5 cut(s) 39, 180, 186, 1516, 1582
BsiWI CGTACG 1 cut(s) 83
BslFI GGGAC 2 cut(s) 792, 1206
BslI CCNNNNNNNGG 5 cut(s) 30, 97, 185, 253, 864
BsmAI GTCTC 4 cut(s) 99, 330, 893, 1497
BsmBI CGTCTC 1 cut(s) 99
BsmFI GGGAC 2 cut(s) 792, 1206
BsmI GAATGC 2 cut(s) 853, 1520
BsnI GGCC 2 cut(s) 178, 245
Bso31I GGTCTC 1 cut(s) 330
BsoBI CYCGRG 1 cut(s) 1515
Bsp1286I GDGCHC 2 cut(s) 1179, 1320
Bsp13I TCCGGA 1 cut(s) 1581
Bsp143I GATC 6 cut(s) 72, 355, 475, 904, 1099, 1441
Bsp1720I GCTNAGC 1 cut(s) 1336
BspACI CCGC 6 cut(s) 63, 198, 371, 396, 551, 649
BspANI GGCC 2 cut(s) 178, 245
BspCNI CTCAG 5 cut(s) 109, 583, 974, 1007, 1636
BspEI TCCGGA 1 cut(s) 1581
BspFNI CGCG 2 cut(s) 107, 398
BspLI GGNNCC 2 cut(s) 184, 196
BspMAI CTGCAG 2 cut(s) 721, 841
BspMI ACCTGC 1 cut(s) 487
BspT107I GGYRCC 1 cut(s) 182
BspTNI GGTCTC 1 cut(s) 330
BsrBI CCGCTC 1 cut(s) 200
BsrDI GCAATG 1 cut(s) 1493
BsrI ACTGG 5 cut(s) 302, 1085, 1200, 1318, 1382
BssECI CCNNGG 6 cut(s) 123, 246, 546, 987, 1514, 1515
BssHII GCGCGC 1 cut(s) 105
BssMI GATC 6 cut(s) 72, 355, 475, 904, 1099, 1441
BssNI GRCGYC 2 cut(s) 183, 231
BssT1I CCWWGG 1 cut(s) 546
Bst2UI CCWGG 5 cut(s) 248, 559, 1153, 1484, 1607
Bst4CI ACNGT 1 cut(s) 124
Bst6I CTCTTC 2 cut(s) 184, 928
BstACI GRCGYC 2 cut(s) 183, 231
BstC8I GCNNGC 3 cut(s) 107, 1035, 1158
BstDEI CTNAG 6 cut(s) 96, 570, 961, 994, 1336, 1644
BstDSI CCRYGG 2 cut(s) 123, 987
BstF5I GGATG 6 cut(s) 901, 1067, 1148, 1204, 1421, 1588
BstFNI CGCG 2 cut(s) 107, 398
BstH2I RGCGCY 2 cut(s) 186, 945
BstHHI GCGC 6 cut(s) 107, 109, 185, 208, 400, 944
BstKTI GATC 6 cut(s) 75, 358, 478, 907, 1102, 1444
BstMAI GTCTC 4 cut(s) 99, 330, 893, 1497
BstMBI GATC 6 cut(s) 72, 355, 475, 904, 1099, 1441
BstMCI CGRYCG 1 cut(s) 75
BstMWI GCNNNNNNNGC 3 cut(s) 203, 836, 839
BstNI CCWGG 5 cut(s) 248, 559, 1153, 1484, 1607
BstSCI CCNGG 9 cut(s) 37, 178, 246, 557, 1151, 1482, 1514, 1515, 1605
BstSFI CTRYAG 2 cut(s) 717, 837
BstSLI GKGCMC 1 cut(s) 1320
BstUI CGCG 2 cut(s) 107, 398
BstV2I GAAGAC 2 cut(s) 756, 768
BstXI CCANNNNNNTGG 1 cut(s) 1120
BsuI GTATCC 2 cut(s) 27, 788
BsuRI GGCC 2 cut(s) 178, 245
BtgI CCRYGG 2 cut(s) 123, 987
BtrI CACGTC 2 cut(s) 79, 319
BtsCI GGATG 6 cut(s) 901, 1067, 1148, 1204, 1421, 1588
BtsIMutI CAGTG 1 cut(s) 1657
BveI ACCTGC 1 cut(s) 487
Cac8I GCNNGC 3 cut(s) 107, 1035, 1158
CfoI GCGC 6 cut(s) 107, 109, 185, 208, 400, 944
Cfr13I GGNCC 3 cut(s) 117, 177, 244
Cfr9I CCCGGG 1 cut(s) 1515
CseI GACGC 1 cut(s) 239
CsiI ACCWGGT 2 cut(s) 557, 1605
Csp6I GTAC 3 cut(s) 84, 157, 1511
CviAII CATG 8 cut(s) 641, 754, 967, 1268, 1286, 1357, 1429, 1544
CviQI GTAC 3 cut(s) 84, 157, 1511
DdeI CTNAG 6 cut(s) 96, 570, 961, 994, 1336, 1644
DinI GGCGCC 1 cut(s) 184
DpnI GATC 6 cut(s) 74, 357, 477, 906, 1101, 1443
DpnII GATC 6 cut(s) 72, 355, 475, 904, 1099, 1441
DraI TTTAAA 2 cut(s) 306, 1053
Eam1104I CTCTTC 2 cut(s) 184, 928
EarI CTCTTC 2 cut(s) 184, 928
EciI GGCGGA 2 cut(s) 386, 566
Eco130I CCWWGG 1 cut(s) 546
Eco24I GRGCYC 1 cut(s) 1179
Eco31I GGTCTC 1 cut(s) 330
Eco47I GGWCC 1 cut(s) 117
Eco47III AGCGCT 1 cut(s) 943
Eco57I CTGAAG 1 cut(s) 1281
Eco88I CYCGRG 1 cut(s) 1515
EcoRII CCWGG 5 cut(s) 246, 557, 1151, 1482, 1605
EcoT14I CCWWGG 1 cut(s) 546
EcoT38I GRGCYC 1 cut(s) 1179
EgeI GGCGCC 1 cut(s) 184
EheI GGCGCC 1 cut(s) 184
ErhI CCWWGG 1 cut(s) 546
Esp3I CGTCTC 1 cut(s) 99
FaeI CATG 8 cut(s) 644, 757, 970, 1271, 1289, 1360, 1432, 1547
FalI AAGNNNNNCTT 2 cut(s) 530, 562
FaqI GGGAC 2 cut(s) 792, 1206
FatI CATG 8 cut(s) 640, 753, 966, 1267, 1285, 1356, 1428, 1543
FauNDI CATATG 1 cut(s) 1088
FbaI TGATCA 3 cut(s) 904, 1099, 1441
FokI GGATG 6 cut(s) 908, 1054, 1135, 1211, 1408, 1575
FriOI GRGCYC 1 cut(s) 1179
FspI TGCGCA 1 cut(s) 207
GlaI GCGC 6 cut(s) 106, 108, 184, 207, 399, 943
GsaI CCCAGC 1 cut(s) 113
HaeII RGCGCY 2 cut(s) 186, 945
HaeIII GGCC 2 cut(s) 178, 245
HapII CCGG 5 cut(s) 39, 180, 186, 1516, 1582
HgaI GACGC 1 cut(s) 239
HhaI GCGC 6 cut(s) 107, 109, 185, 208, 400, 944
Hin1I GRCGYC 2 cut(s) 183, 231
Hin1II CATG 8 cut(s) 644, 757, 970, 1271, 1289, 1360, 1432, 1547
Hin6I GCGC 6 cut(s) 105, 107, 183, 206, 398, 942
HinP1I GCGC 6 cut(s) 105, 107, 183, 206, 398, 942
HindIII AAGCTT 2 cut(s) 1333, 1563
HinfI GANTC 8 cut(s) 515, 710, 911, 992, 1116, 1139, 1474, 1585
HpaII CCGG 5 cut(s) 39, 180, 186, 1516, 1582
HphI GGTGA 6 cut(s) 112, 431, 1301, 1385, 1410, 1451
Hpy166II GTNNAC 4 cut(s) 120, 127, 1418, 1612
Hpy188I TCNGA 6 cut(s) 51, 537, 573, 964, 1138, 1645
Hpy188III TCNNGA 6 cut(s) 224, 525, 631, 707, 908, 1582
Hpy8I GTNNAC 4 cut(s) 120, 127, 1418, 1612
Hpy99I CGWCG 4 cut(s) 80, 230, 233, 443
HpyAV CCTTC 6 cut(s) 160, 532, 670, 764, 769, 1312
HpyCH4III ACNGT 1 cut(s) 124
HpyCH4IV ACGT 2 cut(s) 78, 318
HpyF10VI GCNNNNNNNGC 3 cut(s) 203, 836, 839
HpyF3I CTNAG 6 cut(s) 96, 570, 961, 994, 1336, 1644
HpySE526I ACGT 2 cut(s) 78, 318
Hsp92I GRCGYC 2 cut(s) 183, 231
Hsp92II CATG 8 cut(s) 644, 757, 970, 1271, 1289, 1360, 1432, 1547
HspAI GCGC 6 cut(s) 105, 107, 183, 206, 398, 942
KasI GGCGCC 1 cut(s) 182
Kpn2I TCCGGA 1 cut(s) 1581
Ksp22I TGATCA 3 cut(s) 904, 1099, 1441
Kzo9I GATC 6 cut(s) 72, 355, 475, 904, 1099, 1441
LmnI GCTCC 8 cut(s) 52, 194, 205, 350, 373, 588, 778, 867
LweI GCATC 2 cut(s) 1157, 1645
MabI ACCWGGT 2 cut(s) 557, 1605
MaeII ACGT 2 cut(s) 78, 318
MaeIII GTNAC 2 cut(s) 655, 997
MalI GATC 6 cut(s) 74, 357, 477, 906, 1101, 1443
MbiI CCGCTC 1 cut(s) 200
MboI GATC 6 cut(s) 72, 355, 475, 904, 1099, 1441
MhlI GDGCHC 2 cut(s) 1179, 1320
MluCI AATT 7 cut(s) 7, 54, 459, 693, 1184, 1488, 1531
Mly113I GGCGCC 1 cut(s) 183
MlyI GAGTC 3 cut(s) 719, 986, 1483
MmeI TCCRAC 1 cut(s) 74
MroI TCCGGA 1 cut(s) 1581
MseI TTAA 6 cut(s) 305, 723, 948, 1052, 1568, 1599
MslI CAYNNNNRTG 1 cut(s) 1072
MspI CCGG 5 cut(s) 39, 180, 186, 1516, 1582
MspR9I CCNGG 9 cut(s) 39, 180, 248, 559, 1153, 1484, 1516, 1517, 1607
Mva1269I GAATGC 2 cut(s) 853, 1520
MvaI CCWGG 5 cut(s) 248, 559, 1153, 1484, 1607
MvnI CGCG 2 cut(s) 107, 398
MwoI GCNNNNNNNGC 3 cut(s) 203, 836, 839
NarI GGCGCC 1 cut(s) 183
NciI CCSGG 4 cut(s) 39, 180, 1516, 1517
NdeI CATATG 1 cut(s) 1088
NdeII GATC 6 cut(s) 72, 355, 475, 904, 1099, 1441
NlaIII CATG 8 cut(s) 644, 757, 970, 1271, 1289, 1360, 1432, 1547
NlaIV GGNNCC 2 cut(s) 184, 196
NsbI TGCGCA 1 cut(s) 207
PauI GCGCGC 1 cut(s) 105
PcsI WCGNNNNNNNCGW 2 cut(s) 231, 435
PctI GAATGC 2 cut(s) 853, 1520
PfeI GAWTC 5 cut(s) 515, 911, 1116, 1139, 1585
Pfl23II CGTACG 1 cut(s) 83
Ple19I CGATCG 1 cut(s) 75
PleI GAGTC 3 cut(s) 718, 986, 1482
PluTI GGCGCC 1 cut(s) 186
PpsI GAGTC 3 cut(s) 718, 986, 1482
PsiI TTATAA 1 cut(s) 1406
Psp6I CCWGG 5 cut(s) 246, 557, 1151, 1482, 1605
PspFI CCCAGC 1 cut(s) 109
PspGI CCWGG 5 cut(s) 246, 557, 1151, 1482, 1605
PspLI CGTACG 1 cut(s) 83
PspN4I GGNNCC 2 cut(s) 184, 196
PspPI GGNCC 3 cut(s) 117, 177, 244
PsrI GAACNNNNNNTAC 2 cut(s) 779, 811
PstI CTGCAG 2 cut(s) 721, 841
PteI GCGCGC 1 cut(s) 105
PvuI CGATCG 1 cut(s) 75
RsaI GTAC 3 cut(s) 85, 158, 1512
RsaNI GTAC 3 cut(s) 84, 157, 1511
RseI CAYNNNNRTG 1 cut(s) 1072
SaqAI TTAA 6 cut(s) 305, 723, 948, 1052, 1568, 1599
Sau3AI GATC 6 cut(s) 72, 355, 475, 904, 1099, 1441
Sau96I GGNCC 3 cut(s) 117, 177, 244
SchI GAGTC 3 cut(s) 719, 986, 1483
ScrFI CCNGG 9 cut(s) 39, 180, 248, 559, 1153, 1484, 1516, 1517, 1607
SduI GDGCHC 2 cut(s) 1179, 1320
SexAI ACCWGGT 2 cut(s) 557, 1605
SfaNI GCATC 2 cut(s) 1157, 1645
SfcI CTRYAG 2 cut(s) 717, 837
SfoI GGCGCC 1 cut(s) 184
SinI GGWCC 1 cut(s) 117
SmaI CCCGGG 1 cut(s) 1517
SmiMI CAYNNNNRTG 1 cut(s) 1072
SmlI CTYRAG 1 cut(s) 631
SmoI CTYRAG 1 cut(s) 631
Sse9I AATT 7 cut(s) 7, 54, 459, 693, 1184, 1488, 1531
SsiI CCGC 6 cut(s) 63, 198, 371, 396, 551, 649
SspDI GGCGCC 1 cut(s) 182
StyD4I CCNGG 9 cut(s) 37, 178, 246, 557, 1151, 1482, 1514, 1515, 1605
StyI CCWWGG 1 cut(s) 546
TaaI ACNGT 1 cut(s) 124
TaiI ACGT 2 cut(s) 81, 321
TaqI TCGA 4 cut(s) 75, 225, 1173, 1188
TasI AATT 7 cut(s) 7, 54, 459, 693, 1184, 1488, 1531
TauI GCSGC 3 cut(s) 65, 200, 398
TfiI GAWTC 5 cut(s) 515, 911, 1116, 1139, 1585
Tru1I TTAA 6 cut(s) 305, 723, 948, 1052, 1568, 1599
Tru9I TTAA 6 cut(s) 305, 723, 948, 1052, 1568, 1599
TspDTI ATGAA 6 cut(s) 405, 971, 1056, 1075, 1256, 1380
TspGWI ACGGA 2 cut(s) 1004, 1295
TspMI CCCGGG 1 cut(s) 1515
VpaK11BI GGWCC 1 cut(s) 117
XcmI CCANNNNNNNNNTGG 2 cut(s) 287, 1117
XmaI CCCGGG 1 cut(s) 1515
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.