RLG00000000543

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0009318
Physical Location & Seq
Forward (+)
1869941 .. 1873305
3365 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000000543

Sequence Viewer

Length: 2205 bp
ATGGATAATTTTTGTTTGAAAACCACAACGGGGTTACCCGGAATAGCTCCGACAATTACAGCTGCCCTGCCGATCGACGTGCGTACGAACCCGTCTCAGGTGAGCGCGCTGGGGAGGTCCACCGTGGACAACAATCACAAGAGGTTCTCCTTCAAGTACCCTTTGAAATCTCTCTGGCCCGGCGCCGGAAGAGGAGCCGCTCCTGCGCACAATGGGTTGCCTCTGGACGACGCCGTTCCCTTGGCCCAGGAGGAGGAGGAGGAGGAGGACGACTGCCTTACCCCAACCCAAACCCAAACCCAAAGCGGCAACTGGGTTTTAAAGATTTTGCACGTCAGGTCTCTCTGGGGAGGAGGAGGAGGAGAAGCAGATCAACAGGGTAAGGCGGAGCTGCGTGGGGAAGATGAAGCCGCGCCCAACGATAACAGAAATGGTGATGATGACGAAAATGACGACGATTGTGATGGTTGTAGAATTGAACAACAAGACGATCAACAACAACAACAAGAGCAGCAGGTTGAGTTTGATAGAGATTCGTTTTCCAGATTGCTTCGGAAGGTTTCCTTGGCGGAAGCCAGGTTATATGCTCAGATGTCGTATTTGGGGAGCTTGGCTTACTCCATTCCCAAAATCCAGCCACAAAATCTCTTGAGAGGTCAGGCTCTGCGGTTTGTTACTTCTTCAATAGAAAAGAAGGAACTTGCTGCTAAAATTGAGAAACATCAAGAGTCGGCTGCAGTTGAAGAAACTGAAAGCACCCTAAAGGAAGACATGGAAGAAGACGAGGAAGGGAAGGAGCAGAAGAACAATGGATACAGAATAAGTCCCTCTGCTGCTTATCATATTGCTGCCTCTGCAGCTTCCTATTTGCATTCCCATACAAGGAGCATACTTCCATTCAAATCCTCAAAGGATGAGACTGATCAAGATTCAACAGAAGAAAGCAGAGGAAGAGGCAGCGCTGTTAATATGATGAACTCAGACATGGCTTCTTTGATGGCAACCACGGACTCAGTAACTTCTGTGGTTGCTGCAAAGGAAGAAGTAAAGCAGGCTGTTGCAGATGATTTAAACTCAACACATTCATCCCCGTGCGATTGGTTCATATGTGATGATGATCAAAGTGCCACCAGATTCTTGGTCATTCAGGGGTCCGAATCTCTGGCATCCTGGCAAGCAAATCTACTTTTCGAGCCCATTCAGTTCGAGGGACTGGATGTGCTTGTGCATAGAGGCATATATGAGGCTGCAAAAGGGATTTATGGACAGGTGTTGCCTGAAGTTCATGCACACCTCAAATCTCGTGGTGATAAAGCAACCTTCCGTTTTACTGGGCACTCATTGGGGGGGAGCTTAGCACTATTAATAAACCTCATGCTTCTTATACGGGGTGAAGTGCCAGTTTCTTCATTACTTCCTGTTATAACTTTTGGTTCACCATCCATCATGTGTGGAGGTGATCAACTGCTTCGCAAACTTGGATTACCACAGAGTCATATCCAGGCAATTACATTGCATAGAGACATTGTACCCCGGGCATTTTCTTGCAATTATCCTAATCATGTGGCAGAGCTTCTCAAAGCTTTAAGTGGGAACTTCCGGAATCATCCCTGCCTTAATAACCAGAAGCTACTTTATTGTCCAATGGGGGAGCTGTTGATCCTTCAACCAGATGAGAAATTCTCCCCAAGCCATCATCTGCTGCCTTCAGGCAGCGGTTTATATGTTCTAAGCCTTGCTCTACCAGATGTCAATGATGCAGAGAAGCAGCTGCGTGCAGCAAAGTTGGTATTCTTAAACTCACCACATCCACTTGAAATCTTAAGTGATCGGTCTGCATATGGTTCAGAAGGATGCATTCAAAGAGATCATGACATGAACTCTTACTTAAAATGTGTTCGTGGGGTGATTCGACAAGAGCTTAACCATATTAGGAAGGCCAGAAGACAGCAGAGGCGCAAGGTTTGGTGGCCTCTCGTGTCATCCCGGGGTGTGGATGTTGGAATCATTGTGGGAAGACCTTTTGCGACAATGAAGCAGGGCCAGTACCAGGTCAATTTTTCTGGGGTGTTAGAAAGTGGGAAAGAGTCCTTGAAACGGTTCAGTAGACTGGTTGCATCACAGCACATGCATTTATTTGTGGTGCTTTTGGTACCAGCACGACTCATACTCTTGGGGGCATATAGTGTGATTGGTTTCCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

735

Amino Acids

81.12

Weight (kDa)

5.83

Isoelectric Point (pI)

49.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 380 - 517 2.9e-26 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1424
Acc16I TGCGCA 1 cut(s) 207
Acc36I ACCTGC 1 cut(s) 505
Acc65I GGTACC 1 cut(s) 2152
AccB1I GGYRCC 2 cut(s) 182, 2152
AccBSI CCGCTC 1 cut(s) 200
AccI GTMKAC 1 cut(s) 2107
AccII CGCG 2 cut(s) 107, 413
AccIII TCCGGA 1 cut(s) 1599
AciI CCGC 8 cut(s) 198, 306, 386, 411, 569, 667, 1716, 2200
AclWI GGATC 1 cut(s) 1654
AcsI RAATTY 1 cut(s) 1679
AcuI CTGAAG 2 cut(s) 1299, 1692
AcyI GRCGYC 2 cut(s) 183, 231
AfaI GTAC 5 cut(s) 85, 158, 1530, 2048, 2154
AfeI AGCGCT 1 cut(s) 961
AfiI CCNNNNNNNGG 8 cut(s) 30, 97, 185, 253, 882, 1993, 2050, 2097
AflII CTTAAG 1 cut(s) 1822
AjiI CACGTC 2 cut(s) 79, 334
AjnI CCWGG 5 cut(s) 246, 575, 1169, 1500, 2049
AjuI GAANNNNNNNTTGG 2 cut(s) 548, 580
Alw26I GTCTC 4 cut(s) 99, 345, 911, 1515
AlwI GGATC 1 cut(s) 1654
AlwNI CAGNNNCTG 1 cut(s) 664
Ama87I CYCGRG 2 cut(s) 1533, 1986
Aor13HI TCCGGA 1 cut(s) 1599
Aor51HI AGCGCT 1 cut(s) 961
AoxI GGCC 5 cut(s) 176, 243, 1938, 1970, 2041
ApoI RAATTY 1 cut(s) 1679
AseI ATTAAT 1 cut(s) 1364
Asp700I GAANNNNTTC 1 cut(s) 2099
Asp718I GGTACC 1 cut(s) 2152
AspLEI GCGC 7 cut(s) 107, 109, 185, 208, 415, 962, 1959
AspS9I GGNCC 5 cut(s) 117, 177, 244, 1152, 2041
AsuC2I CCSGG 6 cut(s) 39, 180, 1534, 1535, 1987, 1988
AsuHPI GGTGA 8 cut(s) 112, 446, 1319, 1403, 1428, 1469, 1794, 1918
AvaI CYCGRG 2 cut(s) 1533, 1986
AvaII GGWCC 2 cut(s) 117, 1152
BaeGI GKGCMC 1 cut(s) 1338
BanI GGYRCC 2 cut(s) 182, 2152
BanII GRGCYC 1 cut(s) 1197
BauI CACGAG 2 cut(s) 1302, 1976
BbsI GAAGAC 4 cut(s) 774, 786, 1951, 2023
BccI CCATC 5 cut(s) 458, 991, 1447, 1451, 1701
BceAI ACGGC 1 cut(s) 218
BcgI CGANNNNNNTGC 2 cut(s) 61, 95
BciT130I CCWGG 5 cut(s) 248, 577, 1171, 1502, 2051
BciVI GTATCC 1 cut(s) 806
BclI TGATCA 3 cut(s) 922, 1117, 1459
BcnI CCSGG 6 cut(s) 39, 180, 1534, 1535, 1987, 1988
BcoDI GTCTC 4 cut(s) 99, 345, 911, 1515
BfmI CTRYAG 2 cut(s) 735, 855
BfoI RGCGCY 2 cut(s) 186, 963
BfrI CTTAAG 1 cut(s) 1822
BfuAI ACCTGC 1 cut(s) 505
BfuI GTATCC 1 cut(s) 806
BlpI GCTNAGC 1 cut(s) 1354
Bme18I GGWCC 2 cut(s) 117, 1152
BmeT110I CYCGRG 2 cut(s) 1533, 1986
BmgBI CACGTC 2 cut(s) 79, 334
BmgT120I GGNCC 5 cut(s) 117, 177, 244, 1152, 2041
BmiI GGNNCC 4 cut(s) 184, 196, 1153, 2154
BmrI ACTGGG 2 cut(s) 322, 1341
BmsI GCATC 4 cut(s) 1175, 1747, 1844, 2126
BmuI ACTGGG 2 cut(s) 322, 1341
BpiI GAAGAC 4 cut(s) 774, 786, 1951, 2023
BplI GAGNNNNNCTC 2 cut(s) 1667, 1699
Bpu1102I GCTNAGC 1 cut(s) 1354
BpuEI CTTGAG 1 cut(s) 670
BpuMI CCSGG 6 cut(s) 39, 180, 1534, 1535, 1987, 1988
BsaBI GATNNNNATC 1 cut(s) 1116
BsaHI GRCGYC 2 cut(s) 183, 231
BsaI GGTCTC 1 cut(s) 345
BsaJI CCNNGG 9 cut(s) 123, 240, 246, 564, 1005, 1532, 1533, 1986, 1987
BsaWI WCCGGW 1 cut(s) 1599
Bsc4I CCNNNNNNNGG 8 cut(s) 30, 97, 185, 253, 882, 1993, 2050, 2097
Bse1I ACTGG 6 cut(s) 317, 1218, 1336, 1400, 2044, 2115
Bse3DI GCAATG 1 cut(s) 1511
Bse8I GATNNNNATC 1 cut(s) 1116
BseAI TCCGGA 1 cut(s) 1599
BseBI CCWGG 5 cut(s) 248, 577, 1171, 1502, 2051
BseDI CCNNGG 9 cut(s) 123, 240, 246, 564, 1005, 1532, 1533, 1986, 1987
BseJI GATNNNNATC 1 cut(s) 1116
BseLI CCNNNNNNNGG 8 cut(s) 30, 97, 185, 253, 882, 1993, 2050, 2097
BseMI GCAATG 1 cut(s) 1511
BseMII CTCAG 4 cut(s) 110, 602, 993, 1026
BseNI ACTGG 6 cut(s) 317, 1218, 1336, 1400, 2044, 2115
BsePI GCGCGC 1 cut(s) 105
BseSI GKGCMC 1 cut(s) 1338
BseYI CCCAGC 1 cut(s) 109
BsgI GTGCAG 1 cut(s) 1797
Bsh1236I CGCG 2 cut(s) 107, 413
Bsh1285I CGRYCG 1 cut(s) 75
BshFI GGCC 5 cut(s) 178, 245, 1940, 1972, 2043
BshNI GGYRCC 2 cut(s) 182, 2152
BsiEI CGRYCG 1 cut(s) 75
BsiHKCI CYCGRG 2 cut(s) 1533, 1986
BsiSI CCGG 6 cut(s) 39, 180, 186, 1534, 1600, 1987
BsiWI CGTACG 1 cut(s) 83
BslFI GGGAC 2 cut(s) 810, 1224
BslI CCNNNNNNNGG 8 cut(s) 30, 97, 185, 253, 882, 1993, 2050, 2097
BsmAI GTCTC 4 cut(s) 99, 345, 911, 1515
BsmBI CGTCTC 1 cut(s) 99
BsmFI GGGAC 2 cut(s) 810, 1224
BsmI GAATGC 2 cut(s) 871, 1857
BsnI GGCC 5 cut(s) 178, 245, 1940, 1972, 2043
Bso31I GGTCTC 1 cut(s) 345
BsoBI CYCGRG 2 cut(s) 1533, 1986
Bsp1286I GDGCHC 2 cut(s) 1197, 1338
Bsp13I TCCGGA 1 cut(s) 1599
Bsp143I GATC 9 cut(s) 72, 370, 490, 922, 1117, 1459, 1659, 1828, 1867
Bsp1720I GCTNAGC 1 cut(s) 1354
BspACI CCGC 8 cut(s) 198, 306, 386, 411, 569, 667, 1716, 2200
BspANI GGCC 5 cut(s) 178, 245, 1940, 1972, 2043
BspCNI CTCAG 4 cut(s) 109, 601, 992, 1025
BspEI TCCGGA 1 cut(s) 1599
BspFNI CGCG 2 cut(s) 107, 413
BspHI TCATGA 1 cut(s) 1870
BspLI GGNNCC 4 cut(s) 184, 196, 1153, 2154
BspMAI CTGCAG 2 cut(s) 739, 859
BspMI ACCTGC 1 cut(s) 505
BspPI GGATC 1 cut(s) 1654
BspT107I GGYRCC 2 cut(s) 182, 2152
BspTI CTTAAG 1 cut(s) 1822
BspTNI GGTCTC 1 cut(s) 345
BsrBI CCGCTC 1 cut(s) 200
BsrDI GCAATG 1 cut(s) 1511
BsrI ACTGG 6 cut(s) 317, 1218, 1336, 1400, 2044, 2115
BssECI CCNNGG 9 cut(s) 123, 240, 246, 564, 1005, 1532, 1533, 1986, 1987
BssHII GCGCGC 1 cut(s) 105
BssMI GATC 9 cut(s) 72, 370, 490, 922, 1117, 1459, 1659, 1828, 1867
BssNI GRCGYC 2 cut(s) 183, 231
BssSI CACGAG 2 cut(s) 1302, 1976
BssT1I CCWWGG 2 cut(s) 240, 564
Bst2BI CACGAG 2 cut(s) 1302, 1976
Bst2UI CCWGG 5 cut(s) 248, 577, 1171, 1502, 2051
Bst4CI ACNGT 2 cut(s) 124, 2100
Bst6I CTCTTC 2 cut(s) 184, 946
BstACI GRCGYC 2 cut(s) 183, 231
BstAFI CTTAAG 1 cut(s) 1822
BstC8I GCNNGC 4 cut(s) 107, 1053, 1176, 1776
BstDEI CTNAG 6 cut(s) 96, 588, 979, 1012, 1354, 1730
BstDSI CCRYGG 2 cut(s) 123, 1005
BstEII GGTNACC 1 cut(s) 33
BstFNI CGCG 2 cut(s) 107, 413
BstH2I RGCGCY 2 cut(s) 186, 963
BstHHI GCGC 7 cut(s) 107, 109, 185, 208, 415, 962, 1959
BstKTI GATC 9 cut(s) 75, 373, 493, 925, 1120, 1462, 1662, 1831, 1870
BstMAI GTCTC 4 cut(s) 99, 345, 911, 1515
BstMBI GATC 9 cut(s) 72, 370, 490, 922, 1117, 1459, 1659, 1828, 1867
BstMCI CGRYCG 1 cut(s) 75
BstMWI GCNNNNNNNGC 3 cut(s) 203, 854, 857
BstNI CCWGG 5 cut(s) 248, 577, 1171, 1502, 2051
BstNSI RCATGY 1 cut(s) 2131
BstPI GGTNACC 1 cut(s) 33
BstSFI CTRYAG 2 cut(s) 735, 855
BstSLI GKGCMC 1 cut(s) 1338
BstUI CGCG 2 cut(s) 107, 413
BstV2I GAAGAC 4 cut(s) 774, 786, 1951, 2023
BstXI CCANNNNNNTGG 1 cut(s) 1138
BsuI GTATCC 1 cut(s) 806
BsuRI GGCC 5 cut(s) 178, 245, 1940, 1972, 2043
BtgI CCRYGG 2 cut(s) 123, 1005
BtrI CACGTC 2 cut(s) 79, 334
BveI ACCTGC 1 cut(s) 505
Cac8I GCNNGC 4 cut(s) 107, 1053, 1176, 1776
CaiI CAGNNNCTG 1 cut(s) 664
CciI TCATGA 1 cut(s) 1870
CfoI GCGC 7 cut(s) 107, 109, 185, 208, 415, 962, 1959
Cfr13I GGNCC 5 cut(s) 117, 177, 244, 1152, 2041
Cfr9I CCCGGG 2 cut(s) 1533, 1986
CseI GACGC 1 cut(s) 239
CsiI ACCWGGT 1 cut(s) 2049
Csp6I GTAC 5 cut(s) 84, 157, 1529, 2047, 2153
CspCI CAANNNNNGTGG 2 cut(s) 1285, 1320
CviAII CATG 9 cut(s) 772, 985, 1286, 1375, 1447, 1562, 1871, 1876, 2128
CviQI GTAC 5 cut(s) 84, 157, 1529, 2047, 2153
DdeI CTNAG 6 cut(s) 96, 588, 979, 1012, 1354, 1730
DinI GGCGCC 1 cut(s) 184
DpnI GATC 9 cut(s) 74, 372, 492, 924, 1119, 1461, 1661, 1830, 1869
DpnII GATC 9 cut(s) 72, 370, 490, 922, 1117, 1459, 1659, 1828, 1867
DraI TTTAAA 2 cut(s) 321, 1071
Eam1104I CTCTTC 2 cut(s) 184, 946
EarI CTCTTC 2 cut(s) 184, 946
EciI GGCGGA 2 cut(s) 401, 584
Eco130I CCWWGG 2 cut(s) 240, 564
Eco24I GRGCYC 1 cut(s) 1197
Eco31I GGTCTC 1 cut(s) 345
Eco47I GGWCC 2 cut(s) 117, 1152
Eco47III AGCGCT 1 cut(s) 961
Eco57I CTGAAG 2 cut(s) 1299, 1692
Eco88I CYCGRG 2 cut(s) 1533, 1986
Eco91I GGTNACC 1 cut(s) 33
EcoO65I GGTNACC 1 cut(s) 33
EcoRII CCWGG 5 cut(s) 246, 575, 1169, 1500, 2049
EcoT14I CCWWGG 2 cut(s) 240, 564
EcoT22I ATGCAT 2 cut(s) 1859, 2133
EcoT38I GRGCYC 1 cut(s) 1197
EgeI GGCGCC 1 cut(s) 184
EheI GGCGCC 1 cut(s) 184
ErhI CCWWGG 2 cut(s) 240, 564
Esp3I CGTCTC 1 cut(s) 99
FaeI CATG 9 cut(s) 775, 988, 1289, 1378, 1450, 1565, 1874, 1879, 2131
FalI AAGNNNNNCTT 2 cut(s) 548, 580
FaqI GGGAC 2 cut(s) 810, 1224
FatI CATG 9 cut(s) 771, 984, 1285, 1374, 1446, 1561, 1870, 1875, 2127
FauNDI CATATG 2 cut(s) 1106, 1840
FbaI TGATCA 3 cut(s) 922, 1117, 1459
FblI GTMKAC 1 cut(s) 2107
FriOI GRGCYC 1 cut(s) 1197
FspI TGCGCA 1 cut(s) 207
GlaI GCGC 7 cut(s) 106, 108, 184, 207, 414, 961, 1958
GsaI CCCAGC 1 cut(s) 113
HaeII RGCGCY 2 cut(s) 186, 963
HaeIII GGCC 5 cut(s) 178, 245, 1940, 1972, 2043
HapII CCGG 6 cut(s) 39, 180, 186, 1534, 1600, 1987
HgaI GACGC 1 cut(s) 239
HhaI GCGC 7 cut(s) 107, 109, 185, 208, 415, 962, 1959
Hin1I GRCGYC 2 cut(s) 183, 231
Hin1II CATG 9 cut(s) 775, 988, 1289, 1378, 1450, 1565, 1874, 1879, 2131
Hin6I GCGC 7 cut(s) 105, 107, 183, 206, 413, 960, 1957
HinP1I GCGC 7 cut(s) 105, 107, 183, 206, 413, 960, 1957
HindIII AAGCTT 1 cut(s) 1581
HpaII CCGG 6 cut(s) 39, 180, 186, 1534, 1600, 1987
HphI GGTGA 8 cut(s) 112, 446, 1319, 1403, 1428, 1469, 1794, 1918
Hpy166II GTNNAC 4 cut(s) 120, 127, 1436, 2108
Hpy188I TCNGA 6 cut(s) 51, 555, 591, 982, 1156, 1849
Hpy188III TCNNGA 7 cut(s) 224, 543, 649, 725, 926, 1600, 1871
Hpy8I GTNNAC 4 cut(s) 120, 127, 1436, 2108
Hpy99I CGWCG 3 cut(s) 80, 233, 458
HpyCH4III ACNGT 2 cut(s) 124, 2100
HpyCH4IV ACGT 2 cut(s) 78, 333
HpyF10VI GCNNNNNNNGC 3 cut(s) 203, 854, 857
HpyF3I CTNAG 6 cut(s) 96, 588, 979, 1012, 1354, 1730
HpySE526I ACGT 2 cut(s) 78, 333
Hsp92I GRCGYC 2 cut(s) 183, 231
Hsp92II CATG 9 cut(s) 775, 988, 1289, 1378, 1450, 1565, 1874, 1879, 2131
HspAI GCGC 7 cut(s) 105, 107, 183, 206, 413, 960, 1957
KasI GGCGCC 1 cut(s) 182
Kpn2I TCCGGA 1 cut(s) 1599
KpnI GGTACC 1 cut(s) 2156
Ksp22I TGATCA 3 cut(s) 922, 1117, 1459
Kzo9I GATC 9 cut(s) 72, 370, 490, 922, 1117, 1459, 1659, 1828, 1867
LmnI GCTCC 9 cut(s) 52, 194, 205, 388, 606, 796, 885, 1350, 1651
LweI GCATC 4 cut(s) 1175, 1747, 1844, 2126
MabI ACCWGGT 1 cut(s) 2049
MaeII ACGT 2 cut(s) 78, 333
MaeIII GTNAC 3 cut(s) 33, 673, 1015
MalI GATC 9 cut(s) 74, 372, 492, 924, 1119, 1461, 1661, 1830, 1869
MbiI CCGCTC 1 cut(s) 200
MboI GATC 9 cut(s) 72, 370, 490, 922, 1117, 1459, 1659, 1828, 1867
MhlI GDGCHC 2 cut(s) 1197, 1338
MluCI AATT 8 cut(s) 7, 54, 474, 711, 1506, 1549, 1679, 2056
Mly113I GGCGCC 1 cut(s) 183
MlyI GAGTC 5 cut(s) 737, 1004, 1501, 2096, 2157
MmeI TCCRAC 2 cut(s) 74, 1981
Mph1103I ATGCAT 2 cut(s) 1859, 2133
MroI TCCGGA 1 cut(s) 1599
MroXI GAANNNNTTC 1 cut(s) 2099
MslI CAYNNNNRTG 1 cut(s) 1090
MspA1I CMGCKG 4 cut(s) 62, 1716, 1771, 2202
MspCI CTTAAG 1 cut(s) 1822
MspI CCGG 6 cut(s) 39, 180, 186, 1534, 1600, 1987
Mva1269I GAATGC 2 cut(s) 871, 1857
MvaI CCWGG 5 cut(s) 248, 577, 1171, 1502, 2051
MvnI CGCG 2 cut(s) 107, 413
MwoI GCNNNNNNNGC 3 cut(s) 203, 854, 857
NarI GGCGCC 1 cut(s) 183
NciI CCSGG 6 cut(s) 39, 180, 1534, 1535, 1987, 1988
NdeI CATATG 2 cut(s) 1106, 1840
NdeII GATC 9 cut(s) 72, 370, 490, 922, 1117, 1459, 1659, 1828, 1867
NlaIII CATG 9 cut(s) 775, 988, 1289, 1378, 1450, 1565, 1874, 1879, 2131
NlaIV GGNNCC 4 cut(s) 184, 196, 1153, 2154
NsbI TGCGCA 1 cut(s) 207
NsiI ATGCAT 2 cut(s) 1859, 2133
NspI RCATGY 1 cut(s) 2131
PagI TCATGA 1 cut(s) 1870
PauI GCGCGC 1 cut(s) 105
PcsI WCGNNNNNNNCGW 1 cut(s) 450
PctI GAATGC 2 cut(s) 871, 1857
PdmI GAANNNNTTC 1 cut(s) 2099
PfeI GAWTC 7 cut(s) 533, 929, 1134, 1157, 1603, 1909, 2004
Pfl23II CGTACG 1 cut(s) 83
Ple19I CGATCG 1 cut(s) 75
PleI GAGTC 5 cut(s) 736, 1004, 1500, 2095, 2157
PluTI GGCGCC 1 cut(s) 186
PpsI GAGTC 5 cut(s) 736, 1004, 1500, 2095, 2157
PshBI ATTAAT 1 cut(s) 1364
PsiI TTATAA 1 cut(s) 1424
Psp6I CCWGG 5 cut(s) 246, 575, 1169, 1500, 2049
PspEI GGTNACC 1 cut(s) 33
PspFI CCCAGC 1 cut(s) 109
PspGI CCWGG 5 cut(s) 246, 575, 1169, 1500, 2049
PspLI CGTACG 1 cut(s) 83
PspN4I GGNNCC 4 cut(s) 184, 196, 1153, 2154
PspPI GGNCC 5 cut(s) 117, 177, 244, 1152, 2041
PsrI GAACNNNNNNTAC 2 cut(s) 797, 829
PstI CTGCAG 2 cut(s) 739, 859
PstNI CAGNNNCTG 1 cut(s) 664
PteI GCGCGC 1 cut(s) 105
PvuI CGATCG 1 cut(s) 75
PvuII CAGCTG 2 cut(s) 62, 1771
RsaI GTAC 5 cut(s) 85, 158, 1530, 2048, 2154
RsaNI GTAC 5 cut(s) 84, 157, 1529, 2047, 2153
RseI CAYNNNNRTG 1 cut(s) 1090
Sau3AI GATC 9 cut(s) 72, 370, 490, 922, 1117, 1459, 1659, 1828, 1867
Sau96I GGNCC 5 cut(s) 117, 177, 244, 1152, 2041
SchI GAGTC 5 cut(s) 737, 1004, 1501, 2096, 2157
SduI GDGCHC 2 cut(s) 1197, 1338
SexAI ACCWGGT 1 cut(s) 2049
SfaNI GCATC 4 cut(s) 1175, 1747, 1844, 2126
SfcI CTRYAG 2 cut(s) 735, 855
SfoI GGCGCC 1 cut(s) 184
SinI GGWCC 2 cut(s) 117, 1152
SmaI CCCGGG 2 cut(s) 1535, 1988
SmiMI CAYNNNNRTG 1 cut(s) 1090
SmlI CTYRAG 2 cut(s) 649, 1822
SmoI CTYRAG 2 cut(s) 649, 1822
Sse9I AATT 8 cut(s) 7, 54, 474, 711, 1506, 1549, 1679, 2056
SsiI CCGC 8 cut(s) 198, 306, 386, 411, 569, 667, 1716, 2200
SspDI GGCGCC 1 cut(s) 182
StyI CCWWGG 2 cut(s) 240, 564
TaaI ACNGT 2 cut(s) 124, 2100
TaiI ACGT 2 cut(s) 81, 336
TaqI TCGA 4 cut(s) 75, 1191, 1206, 1912
TaqII GACCGA 1 cut(s) 1821
TasI AATT 8 cut(s) 7, 54, 474, 711, 1506, 1549, 1679, 2056
TauI GCSGC 3 cut(s) 200, 309, 413
TfiI GAWTC 7 cut(s) 533, 929, 1134, 1157, 1603, 1909, 2004
TspDTI ATGAA 8 cut(s) 420, 989, 1074, 1093, 1274, 1398, 1892, 2048
TspGWI ACGGA 2 cut(s) 1022, 1313
TspMI CCCGGG 2 cut(s) 1533, 1986
Vha464I CTTAAG 1 cut(s) 1822
VpaK11BI GGWCC 2 cut(s) 117, 1152
VspI ATTAAT 1 cut(s) 1364
XapI RAATTY 1 cut(s) 1679
XceI RCATGY 1 cut(s) 2131
XcmI CCANNNNNNNNNTGG 1 cut(s) 1135
XmaI CCCGGG 2 cut(s) 1533, 1986
XmiI GTMKAC 1 cut(s) 2107
XmnI GAANNNNTTC 1 cut(s) 2099
Zsp2I ATGCAT 2 cut(s) 1859, 2133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.