Rroxscaffold_3G00218670

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0009318
Physical Location & Seq
Forward (+)
958908 .. 962801
3894 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00218670.1

Sequence Viewer

Length: 2190 bp
ATGGATAATTTTTGTTTGAAAACCACAACGGGGATACCCGGAATAGCTCCGACAATTACAGCTGCCCTGCCGATCGACGTGCGTACGAACCCGTCTCAGGTGAGCGCGCTGGGGAGGTCCGCCGTGGACAACAATCACAAGAGGTTCTCCTTCAAGTACCCTTTGAAATCTCTCTGGCCCGGCGCCGGAAGAGGATCCGCTCCTGCGCACAATGGGTTGCCTCTCGACGACGCCGCTCCCTTGGCCCAGGAGGAGGAGGAGGACGACTGCCTTACCCCAACCCAAACCCAAAGCGGCAACTGGGTTTTAAAGATTTTGCACGTCAGGTCTCTCTGGGGAGGTGGAGGAGGAGCAGATCAACAGGGAAAGGCGGAGCTGCGTGGGGAAGATGAAGCCGCGCCCAACGATAACAGAAATGGTGATGATGACGAAAATGACGACGAATGTGATGGTTGTAGAATTGAACAACAAGACGATGATCAACAACAACAACAAGAGCAGCAGCAGATTGAGTTTGATAGAGATTCGTTTTCCAGATTGCTTCGGAAGGTTTCCTTGGCGGAAGCCAGGTTATATGCTCAGATGTCGTATTTGGGGAGCTTGGCTTACTCCATTCCCAAAATCCAGCCACAAAATCTCTTGAGAGGTCTGCGGTTTGTTACTTCTTCAATAGAAAAGAAGGAACTTGCTGCTAAAATTGAGAAACATCAAGAGTCGGCCGCACTTGAAGAAACTGAAAGCACCCTAAAGGAAGACATGGAAGAAGACGTGGAAGAGAAGGAGCGGAAGAACAATGGATACAGAATAAGTCCCTCTGCTGCTTATCATATTGCTGCCTCTGCAGCTTCCTATTTGCATTCCCATACAAGGAGCATACTTCCATTCAAACCCTCAAAGGATGAGACTGATCAAGATTCAACAGAAGAAAGCAGAGGAAGAGGCAGCGCTGTTAATTTGATGAACTCAGACATGGCTTCTTTGATGGCAACCACGGACTCAGTAACTTCTGTGGTTGCTGCAAAGGAAGAAGTAAAGCAGGCTGTTGCAGATGATTTAAACTCAACACATTCATCCCCGTGTGATTGGTTCATATGTGATGATGATCAAAGTGCCACCAGATTCTTGGTCATTCAGGGGTCTGAATCTCTGGCATCCTGGCAAGCAAATCTACTTTTCGAGCCCATTCAATTCGAGGGACTGGATGTGCTTGTGCATAGAGGCATATATGAGGCTGCAAAAGGGATTTATGGGCAGGTGTTGCCTGAAGTTCATGCACACCTCAAATCTCATGGTGATAAAGCAACCTTCCGTTTTACTGGGCACTCATTAGGGGGAAGCTTAGCACTTCTAATAAACCTCATGCTGCTTATACGGGGTGAAGTGCCAGTTTCTTCATTACTTCCTGTTATAACTTTTGGTTCACCATCCATCATGTGCGGAGGTGATCAACTGCTTCGCAAACTTGGATTACCACAGAGTCATATCCAGGCAATTACATTGCACAGAGACATTGTACCCCGGGCATTCTCTTGCAATTATCCTAATCATGTGGCAGAGCTTCTCAAAGCGTTAAATGGGAACTTCCGGAATCATCCCTGCCTTAATAACCAGAAGCTACTGTATTGTCCAATGGGGGAGCTGTTGATTCTTCAACCAGATGAGAAATTCTCCCCAAACCATCATCTTCTGCCTTCAGGCAGCGGTTTATATGTTCTAAGCGGTGCTCTACCAGATGTCAATGATGCAGAGAAGCAGCTGCGTGCAGCAAAGTTGGTATTCTTAAACTCTCCACATCCACTTGAAATCTTAAGTGATCGGTCTGCATATGGTTCAGAAGGATGCATTCAAAGAGATCATGACATGAACTCTTACTTAAAATGTGTTCGTGGGGTGATTCGACAAGAGCTTAACCATATTAGGAAGGCCAGAAGACAGCAGAGGCGCAAGGTTTGGTGGCCCCTCGTGTCATCCCGGGGTGTGGATGTTGGAATCATTGTGGGAAGGCCTTTTGCAACAATGAAGCAGGGCCAGTACCAGGTCAATTTTTCTGGCGTGTTACAAAGTGGGAAAGAGTCCTTGAAACGGTTCAGTAGACTGGTTGCATCACAGCACATGCATTTGTTTGTGGTGCTTTTGGTACCAGCACGACTCATACTCTTGGGGGCATATAGTGTGATTGGTTTCCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

729

Amino Acids

80.59

Weight (kDa)

6.02

Isoelectric Point (pI)

50.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 375 - 512 1.8e-26 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1409
AarI CACCTGC 1 cut(s) 1243
Acc16I TGCGCA 1 cut(s) 207
Acc36I ACCTGC 1 cut(s) 1243
Acc65I GGTACC 1 cut(s) 2137
AccB1I GGYRCC 2 cut(s) 182, 2137
AccBSI CCGCTC 3 cut(s) 200, 236, 784
AccI GTMKAC 1 cut(s) 2092
AccII CGCG 2 cut(s) 107, 398
AccIII TCCGGA 1 cut(s) 1584
AclWI GGATC 2 cut(s) 189, 202
AcoI YGGCCR 1 cut(s) 717
AcsI RAATTY 1 cut(s) 1664
AcuI CTGAAG 2 cut(s) 1284, 1677
AcyI GRCGYC 2 cut(s) 183, 231
AfaI GTAC 5 cut(s) 85, 158, 1515, 2033, 2139
AfeI AGCGCT 1 cut(s) 946
AfiI CCNNNNNNNGG 8 cut(s) 30, 97, 185, 253, 867, 1978, 2035, 2082
AflII CTTAAG 1 cut(s) 1807
AjiI CACGTC 3 cut(s) 79, 322, 769
AjnI CCWGG 5 cut(s) 246, 566, 1154, 1485, 2034
AjuI GAANNNNNNNTTGG 2 cut(s) 539, 571
Alw21I GWGCWC 1 cut(s) 1726
Alw26I GTCTC 4 cut(s) 99, 333, 896, 1500
AlwI GGATC 2 cut(s) 189, 202
Ama87I CYCGRG 2 cut(s) 1518, 1971
Aor13HI TCCGGA 1 cut(s) 1584
Aor51HI AGCGCT 1 cut(s) 946
AoxI GGCC 7 cut(s) 176, 243, 717, 1923, 1955, 2003, 2026
ApoI RAATTY 1 cut(s) 1664
Asp700I GAANNNNTTC 1 cut(s) 2084
Asp718I GGTACC 1 cut(s) 2137
AspLEI GCGC 7 cut(s) 107, 109, 185, 208, 400, 947, 1944
AspS9I GGNCC 5 cut(s) 117, 177, 244, 1956, 2026
AsuC2I CCSGG 6 cut(s) 39, 180, 1519, 1520, 1972, 1973
AsuHPI GGTGA 7 cut(s) 112, 431, 1304, 1388, 1413, 1454, 1903
AvaI CYCGRG 2 cut(s) 1518, 1971
AvaII GGWCC 1 cut(s) 117
BaeGI GKGCMC 1 cut(s) 1323
BamHI GGATCC 1 cut(s) 194
BanI GGYRCC 2 cut(s) 182, 2137
BanII GRGCYC 1 cut(s) 1182
BauI CACGAG 1 cut(s) 1961
BbsI GAAGAC 3 cut(s) 759, 771, 1936
Bbv12I GWGCWC 1 cut(s) 1726
BccI CCATC 5 cut(s) 443, 976, 1432, 1436, 1686
BceAI ACGGC 1 cut(s) 107
BcgI CGANNNNNNTGC 2 cut(s) 61, 95
BciT130I CCWGG 5 cut(s) 248, 568, 1156, 1487, 2036
BciVI GTATCC 2 cut(s) 27, 791
BclI TGATCA 4 cut(s) 478, 907, 1102, 1444
BcnI CCSGG 6 cut(s) 39, 180, 1519, 1520, 1972, 1973
BcoDI GTCTC 4 cut(s) 99, 333, 896, 1500
BfmI CTRYAG 1 cut(s) 840
BfoI RGCGCY 2 cut(s) 186, 948
BfrI CTTAAG 1 cut(s) 1807
BfuAI ACCTGC 1 cut(s) 1243
BfuI GTATCC 2 cut(s) 27, 791
BlpI GCTNAGC 1 cut(s) 1339
Bme18I GGWCC 1 cut(s) 117
BmeT110I CYCGRG 2 cut(s) 1518, 1971
BmgBI CACGTC 3 cut(s) 79, 322, 769
BmgT120I GGNCC 5 cut(s) 117, 177, 244, 1956, 2026
BmiI GGNNCC 4 cut(s) 184, 196, 1958, 2139
BmrI ACTGGG 2 cut(s) 310, 1326
BmsI GCATC 4 cut(s) 1160, 1732, 1829, 2111
BmuI ACTGGG 2 cut(s) 310, 1326
BpiI GAAGAC 3 cut(s) 759, 771, 1936
BplI GAGNNNNNCTC 2 cut(s) 1652, 1684
Bpu1102I GCTNAGC 1 cut(s) 1339
BpuEI CTTGAG 1 cut(s) 661
BpuMI CCSGG 6 cut(s) 39, 180, 1519, 1520, 1972, 1973
BsaBI GATNNNNATC 1 cut(s) 1101
BsaHI GRCGYC 2 cut(s) 183, 231
BsaI GGTCTC 1 cut(s) 333
BsaJI CCNNGG 9 cut(s) 123, 240, 246, 555, 990, 1517, 1518, 1971, 1972
BsaWI WCCGGW 1 cut(s) 1584
Bsc4I CCNNNNNNNGG 8 cut(s) 30, 97, 185, 253, 867, 1978, 2035, 2082
Bse1I ACTGG 6 cut(s) 305, 1203, 1321, 1385, 2029, 2100
Bse3DI GCAATG 1 cut(s) 1496
Bse8I GATNNNNATC 1 cut(s) 1101
BseAI TCCGGA 1 cut(s) 1584
BseBI CCWGG 5 cut(s) 248, 568, 1156, 1487, 2036
BseDI CCNNGG 9 cut(s) 123, 240, 246, 555, 990, 1517, 1518, 1971, 1972
BseJI GATNNNNATC 1 cut(s) 1101
BseLI CCNNNNNNNGG 8 cut(s) 30, 97, 185, 253, 867, 1978, 2035, 2082
BseMI GCAATG 1 cut(s) 1496
BseMII CTCAG 4 cut(s) 110, 593, 978, 1011
BseNI ACTGG 6 cut(s) 305, 1203, 1321, 1385, 2029, 2100
BsePI GCGCGC 1 cut(s) 105
BseRI GAGGAG 5 cut(s) 266, 269, 272, 360, 363
BseSI GKGCMC 1 cut(s) 1323
BseX3I CGGCCG 1 cut(s) 717
BseYI CCCAGC 1 cut(s) 109
BsgI GTGCAG 1 cut(s) 1782
Bsh1236I CGCG 2 cut(s) 107, 398
Bsh1285I CGRYCG 2 cut(s) 75, 720
BshFI GGCC 7 cut(s) 178, 245, 719, 1925, 1957, 2005, 2028
BshNI GGYRCC 2 cut(s) 182, 2137
BsiEI CGRYCG 2 cut(s) 75, 720
BsiHKAI GWGCWC 1 cut(s) 1726
BsiHKCI CYCGRG 2 cut(s) 1518, 1971
BsiSI CCGG 6 cut(s) 39, 180, 186, 1519, 1585, 1972
BsiWI CGTACG 1 cut(s) 83
BslFI GGGAC 2 cut(s) 795, 1209
BslI CCNNNNNNNGG 8 cut(s) 30, 97, 185, 253, 867, 1978, 2035, 2082
BsmAI GTCTC 4 cut(s) 99, 333, 896, 1500
BsmBI CGTCTC 1 cut(s) 99
BsmFI GGGAC 2 cut(s) 795, 1209
BsmI GAATGC 3 cut(s) 856, 1523, 1842
BsnI GGCC 7 cut(s) 178, 245, 719, 1925, 1957, 2005, 2028
Bso31I GGTCTC 1 cut(s) 333
BsoBI CYCGRG 2 cut(s) 1518, 1971
Bsp1286I GDGCHC 3 cut(s) 1182, 1323, 1726
Bsp13I TCCGGA 1 cut(s) 1584
Bsp143I GATC 9 cut(s) 72, 194, 355, 478, 907, 1102, 1444, 1813, 1852
Bsp1720I GCTNAGC 1 cut(s) 1339
BspANI GGCC 7 cut(s) 178, 245, 719, 1925, 1957, 2005, 2028
BspCNI CTCAG 4 cut(s) 109, 592, 977, 1010
BspEI TCCGGA 1 cut(s) 1584
BspFNI CGCG 2 cut(s) 107, 398
BspHI TCATGA 1 cut(s) 1855
BspLI GGNNCC 4 cut(s) 184, 196, 1958, 2139
BspMAI CTGCAG 1 cut(s) 844
BspMI ACCTGC 1 cut(s) 1243
BspPI GGATC 2 cut(s) 189, 202
BspT107I GGYRCC 2 cut(s) 182, 2137
BspTI CTTAAG 1 cut(s) 1807
BspTNI GGTCTC 1 cut(s) 333
BsrBI CCGCTC 3 cut(s) 200, 236, 784
BsrDI GCAATG 1 cut(s) 1496
BsrI ACTGG 6 cut(s) 305, 1203, 1321, 1385, 2029, 2100
BssECI CCNNGG 9 cut(s) 123, 240, 246, 555, 990, 1517, 1518, 1971, 1972
BssHII GCGCGC 1 cut(s) 105
BssMI GATC 9 cut(s) 72, 194, 355, 478, 907, 1102, 1444, 1813, 1852
BssNI GRCGYC 2 cut(s) 183, 231
BssSI CACGAG 1 cut(s) 1961
BssT1I CCWWGG 2 cut(s) 240, 555
Bst2BI CACGAG 1 cut(s) 1961
Bst2UI CCWGG 5 cut(s) 248, 568, 1156, 1487, 2036
Bst4CI ACNGT 2 cut(s) 1620, 2085
Bst6I CTCTTC 3 cut(s) 184, 768, 931
BstACI GRCGYC 2 cut(s) 183, 231
BstAFI CTTAAG 1 cut(s) 1807
BstAPI GCANNNNNTGC 1 cut(s) 1258
BstC8I GCNNGC 4 cut(s) 107, 1038, 1161, 1761
BstDEI CTNAG 6 cut(s) 96, 579, 964, 997, 1339, 1715
BstDSI CCRYGG 2 cut(s) 123, 990
BstFNI CGCG 2 cut(s) 107, 398
BstH2I RGCGCY 2 cut(s) 186, 948
BstHHI GCGC 7 cut(s) 107, 109, 185, 208, 400, 947, 1944
BstKTI GATC 9 cut(s) 75, 197, 358, 481, 910, 1105, 1447, 1816, 1855
BstMAI GTCTC 4 cut(s) 99, 333, 896, 1500
BstMBI GATC 9 cut(s) 72, 194, 355, 478, 907, 1102, 1444, 1813, 1852
BstMCI CGRYCG 2 cut(s) 75, 720
BstMWI GCNNNNNNNGC 4 cut(s) 242, 839, 842, 1258
BstNI CCWGG 5 cut(s) 248, 568, 1156, 1487, 2036
BstNSI RCATGY 1 cut(s) 2116
BstSFI CTRYAG 1 cut(s) 840
BstSLI GKGCMC 1 cut(s) 1323
BstUI CGCG 2 cut(s) 107, 398
BstV2I GAAGAC 3 cut(s) 759, 771, 1936
BstX2I RGATCY 1 cut(s) 194
BstXI CCANNNNNNTGG 1 cut(s) 1123
BstYI RGATCY 1 cut(s) 194
BstZI CGGCCG 1 cut(s) 717
BsuI GTATCC 2 cut(s) 27, 791
BsuRI GGCC 7 cut(s) 178, 245, 719, 1925, 1957, 2005, 2028
BtgI CCRYGG 2 cut(s) 123, 990
BtrI CACGTC 3 cut(s) 79, 322, 769
BveI ACCTGC 1 cut(s) 1243
Cac8I GCNNGC 4 cut(s) 107, 1038, 1161, 1761
CciI TCATGA 1 cut(s) 1855
CfoI GCGC 7 cut(s) 107, 109, 185, 208, 400, 947, 1944
Cfr13I GGNCC 5 cut(s) 117, 177, 244, 1956, 2026
Cfr9I CCCGGG 2 cut(s) 1518, 1971
CseI GACGC 1 cut(s) 239
CsiI ACCWGGT 1 cut(s) 2034
Csp6I GTAC 5 cut(s) 84, 157, 1514, 2032, 2138
CviQI GTAC 5 cut(s) 84, 157, 1514, 2032, 2138
DdeI CTNAG 6 cut(s) 96, 579, 964, 997, 1339, 1715
DinI GGCGCC 1 cut(s) 184
DpnI GATC 9 cut(s) 74, 196, 357, 480, 909, 1104, 1446, 1815, 1854
DpnII GATC 9 cut(s) 72, 194, 355, 478, 907, 1102, 1444, 1813, 1852
DraI TTTAAA 2 cut(s) 309, 1056
EaeI YGGCCR 1 cut(s) 717
EagI CGGCCG 1 cut(s) 717
Eam1104I CTCTTC 3 cut(s) 184, 768, 931
EarI CTCTTC 3 cut(s) 184, 768, 931
EciI GGCGGA 3 cut(s) 109, 386, 575
EclXI CGGCCG 1 cut(s) 717
Eco130I CCWWGG 2 cut(s) 240, 555
Eco147I AGGCCT 1 cut(s) 2005
Eco24I GRGCYC 1 cut(s) 1182
Eco31I GGTCTC 1 cut(s) 333
Eco47I GGWCC 1 cut(s) 117
Eco47III AGCGCT 1 cut(s) 946
Eco52I CGGCCG 1 cut(s) 717
Eco57I CTGAAG 2 cut(s) 1284, 1677
Eco88I CYCGRG 2 cut(s) 1518, 1971
EcoRII CCWGG 5 cut(s) 246, 566, 1154, 1485, 2034
EcoT14I CCWWGG 2 cut(s) 240, 555
EcoT22I ATGCAT 2 cut(s) 1844, 2118
EcoT38I GRGCYC 1 cut(s) 1182
EgeI GGCGCC 1 cut(s) 184
EheI GGCGCC 1 cut(s) 184
ErhI CCWWGG 2 cut(s) 240, 555
Esp3I CGTCTC 1 cut(s) 99
FalI AAGNNNNNCTT 2 cut(s) 539, 571
FaqI GGGAC 2 cut(s) 795, 1209
FauNDI CATATG 2 cut(s) 1091, 1825
FbaI TGATCA 4 cut(s) 478, 907, 1102, 1444
FblI GTMKAC 1 cut(s) 2092
FriOI GRGCYC 1 cut(s) 1182
FspI TGCGCA 1 cut(s) 207
GlaI GCGC 7 cut(s) 106, 108, 184, 207, 399, 946, 1943
GsaI CCCAGC 1 cut(s) 113
HaeII RGCGCY 2 cut(s) 186, 948
HaeIII GGCC 7 cut(s) 178, 245, 719, 1925, 1957, 2005, 2028
HapII CCGG 6 cut(s) 39, 180, 186, 1519, 1585, 1972
HgaI GACGC 1 cut(s) 239
HhaI GCGC 7 cut(s) 107, 109, 185, 208, 400, 947, 1944
Hin1I GRCGYC 2 cut(s) 183, 231
Hin6I GCGC 7 cut(s) 105, 107, 183, 206, 398, 945, 1942
HinP1I GCGC 7 cut(s) 105, 107, 183, 206, 398, 945, 1942
HindIII AAGCTT 1 cut(s) 1336
HpaII CCGG 6 cut(s) 39, 180, 186, 1519, 1585, 1972
HphI GGTGA 7 cut(s) 112, 431, 1304, 1388, 1413, 1454, 1903
Hpy166II GTNNAC 3 cut(s) 127, 1421, 2093
Hpy188I TCNGA 6 cut(s) 51, 546, 582, 967, 1141, 1834
Hpy188III TCNNGA 7 cut(s) 224, 534, 640, 710, 911, 1585, 1856
Hpy8I GTNNAC 3 cut(s) 127, 1421, 2093
Hpy99I CGWCG 4 cut(s) 80, 230, 233, 443
HpyAV CCTTC 9 cut(s) 160, 541, 673, 772, 1315, 1701, 1829, 1915, 1995
HpyCH4III ACNGT 2 cut(s) 1620, 2085
HpyCH4IV ACGT 3 cut(s) 78, 321, 768
HpyF10VI GCNNNNNNNGC 4 cut(s) 242, 839, 842, 1258
HpyF3I CTNAG 6 cut(s) 96, 579, 964, 997, 1339, 1715
HpySE526I ACGT 3 cut(s) 78, 321, 768
Hsp92I GRCGYC 2 cut(s) 183, 231
HspAI GCGC 7 cut(s) 105, 107, 183, 206, 398, 945, 1942
KasI GGCGCC 1 cut(s) 182
Kpn2I TCCGGA 1 cut(s) 1584
KpnI GGTACC 1 cut(s) 2141
Ksp22I TGATCA 4 cut(s) 478, 907, 1102, 1444
Kzo9I GATC 9 cut(s) 72, 194, 355, 478, 907, 1102, 1444, 1813, 1852
LmnI GCTCC 9 cut(s) 52, 205, 241, 350, 373, 597, 781, 870, 1636
LweI GCATC 4 cut(s) 1160, 1732, 1829, 2111
MabI ACCWGGT 1 cut(s) 2034
MaeII ACGT 3 cut(s) 78, 321, 768
MaeIII GTNAC 3 cut(s) 658, 1000, 2055
MalI GATC 9 cut(s) 74, 196, 357, 480, 909, 1104, 1446, 1815, 1854
MbiI CCGCTC 3 cut(s) 200, 236, 784
MboI GATC 9 cut(s) 72, 194, 355, 478, 907, 1102, 1444, 1813, 1852
MflI RGATCY 1 cut(s) 194
MhlI GDGCHC 3 cut(s) 1182, 1323, 1726
Mly113I GGCGCC 1 cut(s) 183
MlyI GAGTC 5 cut(s) 722, 989, 1486, 2081, 2142
MmeI TCCRAC 2 cut(s) 74, 1966
Mph1103I ATGCAT 2 cut(s) 1844, 2118
MroI TCCGGA 1 cut(s) 1584
MroXI GAANNNNTTC 1 cut(s) 2084
MseI TTAA 9 cut(s) 308, 951, 1055, 1571, 1602, 1781, 1808, 1874, 1908
MslI CAYNNNNRTG 1 cut(s) 1075
MspA1I CMGCKG 4 cut(s) 62, 1701, 1756, 2187
MspCI CTTAAG 1 cut(s) 1807
MspI CCGG 6 cut(s) 39, 180, 186, 1519, 1585, 1972
Mva1269I GAATGC 3 cut(s) 856, 1523, 1842
MvaI CCWGG 5 cut(s) 248, 568, 1156, 1487, 2036
MvnI CGCG 2 cut(s) 107, 398
MwoI GCNNNNNNNGC 4 cut(s) 242, 839, 842, 1258
NarI GGCGCC 1 cut(s) 183
NciI CCSGG 6 cut(s) 39, 180, 1519, 1520, 1972, 1973
NdeI CATATG 2 cut(s) 1091, 1825
NdeII GATC 9 cut(s) 72, 194, 355, 478, 907, 1102, 1444, 1813, 1852
NlaIV GGNNCC 4 cut(s) 184, 196, 1958, 2139
NsbI TGCGCA 1 cut(s) 207
NsiI ATGCAT 2 cut(s) 1844, 2118
NspI RCATGY 1 cut(s) 2116
PagI TCATGA 1 cut(s) 1855
PaqCI CACCTGC 1 cut(s) 1243
PauI GCGCGC 1 cut(s) 105
PceI AGGCCT 1 cut(s) 2005
PcsI WCGNNNNNNNCGW 1 cut(s) 435
PctI GAATGC 3 cut(s) 856, 1523, 1842
PdmI GAANNNNTTC 1 cut(s) 2084
PfeI GAWTC 8 cut(s) 524, 914, 1119, 1142, 1588, 1645, 1894, 1989
Pfl23II CGTACG 1 cut(s) 83
Ple19I CGATCG 1 cut(s) 75
PleI GAGTC 5 cut(s) 721, 989, 1485, 2080, 2142
PluTI GGCGCC 1 cut(s) 186
PpsI GAGTC 5 cut(s) 721, 989, 1485, 2080, 2142
PsiI TTATAA 1 cut(s) 1409
Psp6I CCWGG 5 cut(s) 246, 566, 1154, 1485, 2034
PspFI CCCAGC 1 cut(s) 109
PspGI CCWGG 5 cut(s) 246, 566, 1154, 1485, 2034
PspLI CGTACG 1 cut(s) 83
PspN4I GGNNCC 4 cut(s) 184, 196, 1958, 2139
PspPI GGNCC 5 cut(s) 117, 177, 244, 1956, 2026
PsrI GAACNNNNNNTAC 2 cut(s) 782, 814
PstI CTGCAG 1 cut(s) 844
PsuI RGATCY 1 cut(s) 194
PteI GCGCGC 1 cut(s) 105
PvuI CGATCG 1 cut(s) 75
PvuII CAGCTG 2 cut(s) 62, 1756
RsaI GTAC 5 cut(s) 85, 158, 1515, 2033, 2139
RsaNI GTAC 5 cut(s) 84, 157, 1514, 2032, 2138
RseI CAYNNNNRTG 1 cut(s) 1075
SaqAI TTAA 9 cut(s) 308, 951, 1055, 1571, 1602, 1781, 1808, 1874, 1908
Sau3AI GATC 9 cut(s) 72, 194, 355, 478, 907, 1102, 1444, 1813, 1852
Sau96I GGNCC 5 cut(s) 117, 177, 244, 1956, 2026
SchI GAGTC 5 cut(s) 722, 989, 1486, 2081, 2142
SduI GDGCHC 3 cut(s) 1182, 1323, 1726
SexAI ACCWGGT 1 cut(s) 2034
SfaNI GCATC 4 cut(s) 1160, 1732, 1829, 2111
SfcI CTRYAG 1 cut(s) 840
SfoI GGCGCC 1 cut(s) 184
SinI GGWCC 1 cut(s) 117
SmaI CCCGGG 2 cut(s) 1520, 1973
SmiMI CAYNNNNRTG 1 cut(s) 1075
SmlI CTYRAG 2 cut(s) 640, 1807
SmoI CTYRAG 2 cut(s) 640, 1807
SseBI AGGCCT 1 cut(s) 2005
SspDI GGCGCC 1 cut(s) 182
StuI AGGCCT 1 cut(s) 2005
StyI CCWWGG 2 cut(s) 240, 555
TaaI ACNGT 2 cut(s) 1620, 2085
TaiI ACGT 3 cut(s) 81, 324, 771
TaqI TCGA 5 cut(s) 75, 225, 1176, 1191, 1897
TaqII GACCGA 1 cut(s) 1806
TauI GCSGC 4 cut(s) 236, 297, 398, 722
TfiI GAWTC 8 cut(s) 524, 914, 1119, 1142, 1588, 1645, 1894, 1989
Tru1I TTAA 9 cut(s) 308, 951, 1055, 1571, 1602, 1781, 1808, 1874, 1908
Tru9I TTAA 9 cut(s) 308, 951, 1055, 1571, 1602, 1781, 1808, 1874, 1908
TspDTI ATGAA 8 cut(s) 405, 974, 1059, 1078, 1259, 1383, 1877, 2033
TspGWI ACGGA 2 cut(s) 1007, 1298
TspMI CCCGGG 2 cut(s) 1518, 1971
Vha464I CTTAAG 1 cut(s) 1807
VpaK11BI GGWCC 1 cut(s) 117
XapI RAATTY 1 cut(s) 1664
XceI RCATGY 1 cut(s) 2116
XcmI CCANNNNNNNNNTGG 1 cut(s) 1120
XmaI CCCGGG 2 cut(s) 1518, 1971
XmiI GTMKAC 1 cut(s) 2092
XmnI GAANNNNTTC 1 cut(s) 2084
Zsp2I ATGCAT 2 cut(s) 1844, 2118
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.