MD11G1316500.v1.1

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0009318
Physical Location & Seq
Reverse (-)
42815375 .. 42819011
3637 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1316500.v1.1.491

Sequence Viewer

Length: 2220 bp
ATGGATGCTCTTTGTCTGAAAACAACTGCGCCAGGGATACACGGCCTATCTTCCACCATCTCAGCGGCTGCAGCCCTCGATGTCCGTACGAGCCCATCTCCGTCTCAGGTGAGCGCCATCGGCTGCTCCTCCGCATCTGGGATTGACAAGTCAACCGTAACCGCGGCTCAGAAAAGGATAACGCCGTTTTCCAGATTCTCATTCAAGCACCCTCTGAAATCCTTATGGCCTGGAGCAGCAAGAGGTGGTGGACCCGCCTACAATGGGATGGCTCTCGACGACGCCGTCCCCTTGTCCTCTAAGGAGGAGGGCGAGGAGGAGGAGCTCCAGAGTGCGACCAGGGCGTCGGACGGTCAAAGTGGGAACTGGGTTTTGAAGATTTTGCACGTGAGGTCTCTTTCTCTACCCAGGAGAGAGGAACAGCAGGGAGGCAAGAGTGGCGGCGTTGCAGAGCTACCCACGGGAGATAGAAAAACCATGGTGGATGATGACTCGGAGGAAGACGATGACGAAAATAATTGTTGTAGTGTTTCAGATGAGGCCGAGTTTGATAAAGACACGTTTCGTCGGTTGCTTCGGAAGGTCTCGTTGGCGGAGGCGAGGTTTTATGCTCAAATGTCGTATTTGGGGAGCTTAGCTTATTCCATTCCTAAAATCCAGACGGGAAATCTTCTAAGAAGGTATGGTCTGCGTTTTGTTACTTCTTCAGTAGAGAAGAAGGAATTGACTGCTAAAACTGAGAAAGATCAGGGGTCAGCTGAGATTCGAGAAACTGAAAAAAACCTTAAGGAAGACAAGGAAGAAGATGCGGAAGTAAAGGAGCAAAAGAACTATGGATATCGAATAAGTCCATCTGCTGCTTATCATATTGCTGCTTCTGCAGCTTCCTATCTGCATTCTCAAACAAGGAGCATACTTCCATTCAAATCCTCAGAGACCGAGGCAGATAAAGACTACCATGAAGAAGGCAGTGAAAGAAGTGAAGCTGTTAATAGGATGAACTCAGAAGTGGCTTCTTTAATGGCAACCACCGACTCAGTTACAGCCGTGGTTGCTGCAAAGGAAGAAGTAAAGCAGGCTGTTGCAGATGACTTAAATTCAACAATTTCATCACCTTGTGAATGGTTCGTATGTGATGATGATCGAAGTGGCACCAGATTCTTTGTCATTCAGGGGTCTGAATCACTAGCATCCTGGCAAGCAAATCTACTTTTTGAGCCCATTCAGTTTGAGGGACTGGATGTGCTTGTGCATAGAGGCATATACGAGGCTGCGAAAGGGATATACGAACAGATGCTGCCTGAAGTCCATGCCCACCTCAAATCTCGTGGTGACCATGCAACCTTTCGTTATACTGGGCATTCTTTAGGGGGAAGCTTGGCACTCTTGATAAATCTCATGTTGCTTATACGGCTAGAAGTGCCAATTTCTTCCTTGCTTCCTGTTATTACTTTTGGTGCACCATCCATTATGTGTGGAGGTGATCAATTGCTTCACAAGCTTGGATTACCGCGAAGTCATGTTCAGTCTATTATGTTGCACAGAGACATTGTACCCCGTGCCTTCTCTTGCAATTACCCAAATCATGTGGCAGAGCTTCTCAAGGCTTTGAATGGGAACTTTCGGAATCATCCCTGTCTCAATAACCAGAAGCTACTGTATTCTCCAATGGGGGAGATGCTGATTCTTCAACCAGATGAGAAATTCTCCCCAAATCATCATCTTCTTCCATCGGGCAGCGGTCTGTATCTTCTAAGCTGTCCTCTGTCTGATGGCAGTGATGCAGAGAAGCAGCTCCGCACTGCACAGTTGGTATTCCTAAACTCACCACACCCACTTGAAATCTTAAGTGATCGGTCTGCATATGGTTCAGAAGGAACCATTCAAAGAGATCATGATGTGAATTCTTACTTAAAATCTGTTCGCGGGGTAATTCGGGAAGAGCTTAGCCAGATCAGAAAGGCCAGGAGACAGCAGCGCCGCAAGGTGTGGTGGCCCTTGATAGCACCCCGGAGCAAGCATGCAGGAGGTGTGATTGTGGGAATCAACCTGGGGCAAGAAGAGCAATTAAACTTTTCAGGTGCATTACAGACCGGAAAAGAATCGTTGAAACGGTTCAGTAGACTTGTTGCATGGGAGCACATGCATTTGTTTGTGGTGCTTTTGGTGCCGGCGCGATTGTTAGTCTTGGGAGCATACAGCATCATCAGTTTCCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

740

Amino Acids

81.48

Weight (kDa)

6.32

Isoelectric Point (pI)

48.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 388 - 525 8.1e-25 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 284, 343
AccB1I GGYRCC 2 cut(s) 1151, 2167
AccI GTMKAC 1 cut(s) 2122
AccII CGCG 4 cut(s) 164, 1513, 1926, 2176
AcsI RAATTY 3 cut(s) 1096, 1703, 1903
AcuI CTGAAG 2 cut(s) 690, 1323
AcvI CACGTG 1 cut(s) 388
AcyI GRCGYC 2 cut(s) 282, 344
AdeI CACNNNGTG 1 cut(s) 1118
AfaI GTAC 2 cut(s) 88, 1554
AfiI CCNNNNNNNGG 2 cut(s) 138, 264
AflII CTTAAG 2 cut(s) 785, 1846
AflIII ACRYGT 1 cut(s) 558
AgsI TTSAA 9 cut(s) 205, 376, 925, 1101, 1612, 1691, 1841, 1886, 2110
AjnI CCWGG 7 cut(s) 31, 229, 338, 407, 1193, 1964, 2049
AjuI GAANNNNNNNTTGG 2 cut(s) 572, 604
Alw21I GWGCWC 3 cut(s) 327, 1462, 2142
Alw26I GTCTC 7 cut(s) 108, 399, 589, 929, 1539, 1643, 1963
Alw44I GTGCAC 1 cut(s) 1458
AlwNI CAGNNNCTG 2 cut(s) 68, 1297
AoxI GGCC 5 cut(s) 43, 227, 540, 1962, 1994
ApaLI GTGCAC 1 cut(s) 1458
ApoI RAATTY 3 cut(s) 1096, 1703, 1903
Asp700I GAANNNNTTC 1 cut(s) 2114
AspLEI GCGC 4 cut(s) 31, 116, 1980, 2176
AspS9I GGNCC 2 cut(s) 251, 1995
AsuC2I CCSGG 1 cut(s) 2011
AsuHPI GGTGA 5 cut(s) 121, 1104, 1343, 1493, 1818
AvaII GGWCC 1 cut(s) 251
BaeGI GKGCMC 1 cut(s) 1462
BanI GGYRCC 2 cut(s) 1151, 2167
BanII GRGCYC 3 cut(s) 95, 327, 1221
BauI CACGAG 1 cut(s) 1326
BbrPI CACGTG 1 cut(s) 388
BbsI GAAGAC 2 cut(s) 507, 798
Bbv12I GWGCWC 3 cut(s) 327, 1462, 2142
BccI CCATC 8 cut(s) 65, 103, 125, 262, 859, 1471, 1738, 1766
BceAI ACGGC 5 cut(s) 58, 169, 269, 1031, 1427
BciT130I CCWGG 7 cut(s) 33, 231, 340, 409, 1195, 1966, 2051
BciVI GTATCC 1 cut(s) 30
BclI TGATCA 1 cut(s) 1483
BcnI CCSGG 1 cut(s) 2011
BcoDI GTCTC 7 cut(s) 108, 399, 589, 929, 1539, 1643, 1963
BfaI CTAG 2 cut(s) 1187, 1415
BfmI CTRYAG 2 cut(s) 69, 879
BfoI RGCGCY 2 cut(s) 117, 1981
BfrI CTTAAG 2 cut(s) 785, 1846
BfuI GTATCC 1 cut(s) 30
BlpI GCTNAGC 2 cut(s) 634, 1946
Bme1390I CCNGG 8 cut(s) 33, 231, 340, 409, 1195, 1966, 2011, 2051
Bme18I GGWCC 1 cut(s) 251
BmgT120I GGNCC 2 cut(s) 251, 1995
BmiI GGNNCC 4 cut(s) 253, 1153, 1879, 2169
BmrFI CCNGG 8 cut(s) 33, 231, 340, 409, 1195, 1966, 2011, 2051
BmrI ACTGGG 2 cut(s) 376, 1365
BmsI GCATC 7 cut(s) 143, 796, 1199, 1284, 1668, 1771, 2211
BmuI ACTGGG 2 cut(s) 376, 1365
BpiI GAAGAC 2 cut(s) 507, 798
BplI GAGNNNNNCTC 4 cut(s) 82, 114, 1691, 1723
BpmI CTGGAG 2 cut(s) 252, 311
Bpu1102I GCTNAGC 2 cut(s) 634, 1946
BpuEI CTTGAG 1 cut(s) 1586
BpuMI CCSGG 1 cut(s) 2011
BsaAI YACGTR 1 cut(s) 388
BsaBI GATNNNNATC 1 cut(s) 1140
BsaHI GRCGYC 2 cut(s) 282, 344
BsaI GGTCTC 3 cut(s) 399, 589, 929
BsaWI WCCGGW 2 cut(s) 2093, 2214
BsaXI ACNNNNNCTCC 2 cut(s) 587, 617
Bsc4I CCNNNNNNNGG 2 cut(s) 138, 264
Bse118I RCCGGY 1 cut(s) 2170
Bse1I ACTGG 3 cut(s) 371, 1242, 1360
Bse8I GATNNNNATC 1 cut(s) 1140
BseBI CCWGG 7 cut(s) 33, 231, 340, 409, 1195, 1966, 2051
BseGI GGATG 8 cut(s) 10, 273, 490, 1002, 1190, 1246, 1463, 1630
BseJI GATNNNNATC 1 cut(s) 1140
BseLI CCNNNNNNNGG 2 cut(s) 138, 264
BseMII CTCAG 8 cut(s) 75, 119, 182, 729, 750, 945, 1017, 1050
BseNI ACTGG 3 cut(s) 371, 1242, 1360
BseRI GAGGAG 5 cut(s) 118, 320, 329, 332, 335
BseSI GKGCMC 1 cut(s) 1462
BsgI GTGCAG 1 cut(s) 1788
Bsh1236I CGCG 4 cut(s) 164, 1513, 1926, 2176
BshFI GGCC 5 cut(s) 45, 229, 542, 1964, 1996
BshNI GGYRCC 2 cut(s) 1151, 2167
BsiHKAI GWGCWC 3 cut(s) 327, 1462, 2142
BsiSI CCGG 4 cut(s) 2011, 2094, 2171, 2215
BsiWI CGTACG 1 cut(s) 86
BslFI GGGAC 2 cut(s) 272, 1248
BslI CCNNNNNNNGG 2 cut(s) 138, 264
BsmAI GTCTC 7 cut(s) 108, 399, 589, 929, 1539, 1643, 1963
BsmBI CGTCTC 1 cut(s) 108
BsmFI GGGAC 2 cut(s) 272, 1248
BsmI GAATGC 2 cut(s) 895, 1360
BsnI GGCC 5 cut(s) 45, 229, 542, 1964, 1996
Bso31I GGTCTC 3 cut(s) 399, 589, 929
Bsp1286I GDGCHC 5 cut(s) 95, 327, 1221, 1462, 2142
Bsp143I GATC 6 cut(s) 745, 1141, 1483, 1852, 1891, 1953
Bsp1720I GCTNAGC 2 cut(s) 634, 1946
Bsp19I CCATGG 1 cut(s) 477
BspANI GGCC 5 cut(s) 45, 229, 542, 1964, 1996
BspCNI CTCAG 8 cut(s) 74, 118, 181, 730, 751, 944, 1016, 1049
BspFNI CGCG 4 cut(s) 164, 1513, 1926, 2176
BspHI TCATGA 1 cut(s) 1894
BspLI GGNNCC 4 cut(s) 253, 1153, 1879, 2169
BspMAI CTGCAG 2 cut(s) 73, 883
BspQI GCTCTTC 2 cut(s) 1935, 2055
BspT107I GGYRCC 2 cut(s) 1151, 2167
BspTI CTTAAG 2 cut(s) 785, 1846
BspTNI GGTCTC 3 cut(s) 399, 589, 929
BsrFI RCCGGY 1 cut(s) 2170
BsrI ACTGG 3 cut(s) 371, 1242, 1360
BssAI RCCGGY 1 cut(s) 2170
BssMI GATC 6 cut(s) 745, 1141, 1483, 1852, 1891, 1953
BssNI GRCGYC 2 cut(s) 282, 344
BssSI CACGAG 1 cut(s) 1326
BssT1I CCWWGG 1 cut(s) 477
Bst2BI CACGAG 1 cut(s) 1326
Bst2UI CCWGG 7 cut(s) 33, 231, 340, 409, 1195, 1966, 2051
Bst4CI ACNGT 5 cut(s) 157, 353, 1659, 1809, 2115
Bst6I CTCTTC 2 cut(s) 1935, 2055
BstACI GRCGYC 2 cut(s) 282, 344
BstAFI CTTAAG 2 cut(s) 785, 1846
BstBAI YACGTR 1 cut(s) 388
BstC8I GCNNGC 5 cut(s) 1077, 1200, 2018, 2022, 2172
BstDSI CCRYGG 4 cut(s) 162, 459, 477, 1047
BstEII GGTNACC 1 cut(s) 1331
BstF5I GGATG 8 cut(s) 10, 273, 490, 1002, 1190, 1246, 1463, 1630
BstFNI CGCG 4 cut(s) 164, 1513, 1926, 2176
BstH2I RGCGCY 2 cut(s) 117, 1981
BstHHI GCGC 4 cut(s) 31, 116, 1980, 2176
BstKTI GATC 6 cut(s) 748, 1144, 1486, 1855, 1894, 1956
BstMAI GTCTC 7 cut(s) 108, 399, 589, 929, 1539, 1643, 1963
BstMBI GATC 6 cut(s) 745, 1141, 1483, 1852, 1891, 1953
BstNI CCWGG 7 cut(s) 33, 231, 340, 409, 1195, 1966, 2051
BstNSI RCATGY 2 cut(s) 2024, 2146
BstPI GGTNACC 1 cut(s) 1331
BstSCI CCNGG 8 cut(s) 31, 229, 338, 407, 1193, 1964, 2009, 2049
BstSFI CTRYAG 2 cut(s) 69, 879
BstSLI GKGCMC 1 cut(s) 1462
BstUI CGCG 4 cut(s) 164, 1513, 1926, 2176
BstV2I GAAGAC 2 cut(s) 507, 798
BsuI GTATCC 1 cut(s) 30
BsuRI GGCC 5 cut(s) 45, 229, 542, 1964, 1996
BtgI CCRYGG 4 cut(s) 162, 459, 477, 1047
BtsCI GGATG 8 cut(s) 10, 273, 490, 1002, 1190, 1246, 1463, 1630
BtsI GCAGTG 3 cut(s) 976, 1783, 1800
BtsIMutI CAGTG 3 cut(s) 976, 1783, 1800
Cac8I GCNNGC 5 cut(s) 1077, 1200, 2018, 2022, 2172
CaiI CAGNNNCTG 2 cut(s) 68, 1297
CciI TCATGA 1 cut(s) 1894
CfoI GCGC 4 cut(s) 31, 116, 1980, 2176
Cfr10I RCCGGY 1 cut(s) 2170
Cfr13I GGNCC 2 cut(s) 251, 1995
Cfr42I CCGCGG 1 cut(s) 165
CseI GACGC 2 cut(s) 290, 333
Csp6I GTAC 2 cut(s) 87, 1553
CspCI CAANNNNNGTGG 4 cut(s) 1309, 1344, 1569, 1604
CviQI GTAC 2 cut(s) 87, 1553
DpnI GATC 6 cut(s) 747, 1143, 1485, 1854, 1893, 1955
DpnII GATC 6 cut(s) 745, 1141, 1483, 1852, 1891, 1953
DraIII CACNNNGTG 1 cut(s) 1118
DrdI GACNNNNNNGTC 2 cut(s) 284, 343
DseDI GACNNNNNNGTC 2 cut(s) 284, 343
Eam1104I CTCTTC 2 cut(s) 1935, 2055
EarI CTCTTC 2 cut(s) 1935, 2055
EciI GGCGGA 1 cut(s) 608
Ecl136II GAGCTC 1 cut(s) 325
Eco130I CCWWGG 1 cut(s) 477
Eco24I GRGCYC 3 cut(s) 95, 327, 1221
Eco31I GGTCTC 3 cut(s) 399, 589, 929
Eco32I GATATC 1 cut(s) 839
Eco47I GGWCC 1 cut(s) 251
Eco53kI GAGCTC 1 cut(s) 325
Eco57I CTGAAG 2 cut(s) 690, 1323
Eco72I CACGTG 1 cut(s) 388
Eco91I GGTNACC 1 cut(s) 1331
EcoICRI GAGCTC 1 cut(s) 325
EcoO65I GGTNACC 1 cut(s) 1331
EcoRI GAATTC 1 cut(s) 1903
EcoRII CCWGG 7 cut(s) 31, 229, 338, 407, 1193, 1964, 2049
EcoRV GATATC 1 cut(s) 839
EcoT14I CCWWGG 1 cut(s) 477
EcoT22I ATGCAT 1 cut(s) 2148
EcoT38I GRGCYC 3 cut(s) 95, 327, 1221
ErhI CCWWGG 1 cut(s) 477
Esp3I CGTCTC 1 cut(s) 108
FaqI GGGAC 2 cut(s) 272, 1248
FauI CCCGC 2 cut(s) 262, 1919
FauNDI CATATG 1 cut(s) 1864
FbaI TGATCA 1 cut(s) 1483
FblI GTMKAC 1 cut(s) 2122
FokI GGATG 8 cut(s) 17, 280, 497, 1009, 1177, 1253, 1450, 1617
FriOI GRGCYC 3 cut(s) 95, 327, 1221
FspBI CTAG 2 cut(s) 1187, 1415
GlaI GCGC 4 cut(s) 30, 115, 1979, 2175
GsuI CTGGAG 2 cut(s) 252, 311
HaeII RGCGCY 2 cut(s) 117, 1981
HaeIII GGCC 5 cut(s) 45, 229, 542, 1964, 1996
HapII CCGG 4 cut(s) 2011, 2094, 2171, 2215
HgaI GACGC 2 cut(s) 290, 333
HhaI GCGC 4 cut(s) 31, 116, 1980, 2176
Hin1I GRCGYC 2 cut(s) 282, 344
Hin6I GCGC 4 cut(s) 29, 114, 1978, 2174
HinP1I GCGC 4 cut(s) 29, 114, 1978, 2174
HincII GTYRAC 1 cut(s) 153
HindII GTYRAC 1 cut(s) 153
HindIII AAGCTT 2 cut(s) 1375, 1499
HpaII CCGG 4 cut(s) 2011, 2094, 2171, 2215
HphI GGTGA 5 cut(s) 121, 1104, 1343, 1493, 1818
Hpy166II GTNNAC 4 cut(s) 153, 251, 1460, 2123
Hpy188III TCNNGA 8 cut(s) 192, 275, 328, 658, 767, 1387, 1895, 1937
Hpy8I GTNNAC 4 cut(s) 153, 251, 1460, 2123
Hpy99I CGWCG 4 cut(s) 281, 284, 349, 570
HpyAV CCTTC 6 cut(s) 574, 672, 712, 959, 1573, 1868
HpyCH4III ACNGT 5 cut(s) 157, 353, 1659, 1809, 2115
HpyCH4IV ACGT 2 cut(s) 387, 560
HpySE526I ACGT 2 cut(s) 387, 560
Hsp92I GRCGYC 2 cut(s) 282, 344
HspAI GCGC 4 cut(s) 29, 114, 1978, 2174
KroI GCCGGC 1 cut(s) 2170
KroNI GCCGGC 1 cut(s) 2172
Ksp22I TGATCA 1 cut(s) 1483
KspI CCGCGG 1 cut(s) 165
Kzo9I GATC 6 cut(s) 745, 1141, 1483, 1852, 1891, 1953
LguI GCTCTTC 2 cut(s) 1935, 2055
LweI GCATC 7 cut(s) 143, 796, 1199, 1284, 1668, 1771, 2211
MaeI CTAG 2 cut(s) 1187, 1415
MaeII ACGT 2 cut(s) 387, 560
MaeIII GTNAC 4 cut(s) 157, 697, 1039, 1331
MalI GATC 6 cut(s) 747, 1143, 1485, 1854, 1893, 1955
MboI GATC 6 cut(s) 745, 1141, 1483, 1852, 1891, 1953
MfeI CAATTG 1 cut(s) 1487
MhlI GDGCHC 5 cut(s) 95, 327, 1221, 1462, 2142
MlyI GAGTC 2 cut(s) 485, 1028
MmeI TCCRAC 1 cut(s) 327
Mph1103I ATGCAT 1 cut(s) 2148
MroNI GCCGGC 1 cut(s) 2170
MroXI GAANNNNTTC 1 cut(s) 2114
MseI TTAA 7 cut(s) 786, 990, 1019, 1094, 1847, 1913, 2069
MspA1I CMGCKG 4 cut(s) 65, 164, 758, 1740
MspCI CTTAAG 2 cut(s) 785, 1846
MspI CCGG 4 cut(s) 2011, 2094, 2171, 2215
MspR9I CCNGG 8 cut(s) 33, 231, 340, 409, 1195, 1966, 2011, 2051
MunI CAATTG 1 cut(s) 1487
Mva1269I GAATGC 2 cut(s) 895, 1360
MvaI CCWGG 7 cut(s) 33, 231, 340, 409, 1195, 1966, 2051
MvnI CGCG 4 cut(s) 164, 1513, 1926, 2176
NaeI GCCGGC 1 cut(s) 2172
NciI CCSGG 1 cut(s) 2011
NcoI CCATGG 1 cut(s) 477
NdeI CATATG 1 cut(s) 1864
NdeII GATC 6 cut(s) 745, 1141, 1483, 1852, 1891, 1953
NgoMIV GCCGGC 1 cut(s) 2170
NlaIV GGNNCC 4 cut(s) 253, 1153, 1879, 2169
NmeAIII GCCGAG 1 cut(s) 568
NmuCI GTSAC 1 cut(s) 1331
NsiI ATGCAT 1 cut(s) 2148
NspI RCATGY 2 cut(s) 2024, 2146
PaeI GCATGC 1 cut(s) 2024
PagI TCATGA 1 cut(s) 1894
PciSI GCTCTTC 2 cut(s) 1935, 2055
PcsI WCGNNNNNNNCGW 1 cut(s) 282
PctI GAATGC 2 cut(s) 895, 1360
PdiI GCCGGC 1 cut(s) 2172
PdmI GAANNNNTTC 1 cut(s) 2114
PfeI GAWTC 8 cut(s) 195, 763, 1158, 1181, 1627, 1684, 2043, 2102
Pfl23II CGTACG 1 cut(s) 86
PflFI GACNNNGTC 1 cut(s) 284
PleI GAGTC 2 cut(s) 485, 1028
PmaCI CACGTG 1 cut(s) 388
PmlI CACGTG 1 cut(s) 388
PpsI GAGTC 2 cut(s) 485, 1028
Ppu21I YACGTR 1 cut(s) 388
Psp124BI GAGCTC 1 cut(s) 327
Psp6I CCWGG 7 cut(s) 31, 229, 338, 407, 1193, 1964, 2049
PspCI CACGTG 1 cut(s) 388
PspEI GGTNACC 1 cut(s) 1331
PspGI CCWGG 7 cut(s) 31, 229, 338, 407, 1193, 1964, 2049
PspLI CGTACG 1 cut(s) 86
PspN4I GGNNCC 4 cut(s) 253, 1153, 1879, 2169
PspPI GGNCC 2 cut(s) 251, 1995
PstI CTGCAG 2 cut(s) 73, 883
PstNI CAGNNNCTG 2 cut(s) 68, 1297
PsyI GACNNNGTC 1 cut(s) 284
PvuII CAGCTG 1 cut(s) 758
RsaI GTAC 2 cut(s) 88, 1554
RsaNI GTAC 2 cut(s) 87, 1553
SacI GAGCTC 1 cut(s) 327
SacII CCGCGG 1 cut(s) 165
SapI GCTCTTC 2 cut(s) 1935, 2055
SaqAI TTAA 7 cut(s) 786, 990, 1019, 1094, 1847, 1913, 2069
Sau3AI GATC 6 cut(s) 745, 1141, 1483, 1852, 1891, 1953
Sau96I GGNCC 2 cut(s) 251, 1995
SchI GAGTC 2 cut(s) 485, 1028
ScrFI CCNGG 8 cut(s) 33, 231, 340, 409, 1195, 1966, 2011, 2051
SduI GDGCHC 5 cut(s) 95, 327, 1221, 1462, 2142
SfaNI GCATC 7 cut(s) 143, 796, 1199, 1284, 1668, 1771, 2211
SfcI CTRYAG 2 cut(s) 69, 879
Sfr303I CCGCGG 1 cut(s) 165
SgrBI CCGCGG 1 cut(s) 165
SinI GGWCC 1 cut(s) 251
SmlI CTYRAG 3 cut(s) 785, 1601, 1846
SmoI CTYRAG 3 cut(s) 785, 1601, 1846
SphI GCATGC 1 cut(s) 2024
SspMI CTAG 2 cut(s) 1187, 1415
SstI GAGCTC 1 cut(s) 327
StyD4I CCNGG 8 cut(s) 31, 229, 338, 407, 1193, 1964, 2009, 2049
StyI CCWWGG 1 cut(s) 477
TaaI ACNGT 5 cut(s) 157, 353, 1659, 1809, 2115
TaiI ACGT 2 cut(s) 390, 563
TaqI TCGA 5 cut(s) 78, 276, 766, 841, 1144
TaqII GACCGA 2 cut(s) 953, 1845
TauI GCSGC 4 cut(s) 68, 167, 444, 1983
TfiI GAWTC 8 cut(s) 195, 763, 1158, 1181, 1627, 1684, 2043, 2102
Tru1I TTAA 7 cut(s) 786, 990, 1019, 1094, 1847, 1913, 2069
Tru9I TTAA 7 cut(s) 786, 990, 1019, 1094, 1847, 1913, 2069
TscAI CASTG 3 cut(s) 976, 1783, 1807
TseFI GTSAC 1 cut(s) 1331
Tsp45I GTSAC 1 cut(s) 1331
TspDTI ATGAA 3 cut(s) 975, 1013, 1098
TspGWI ACGGA 2 cut(s) 74, 90
TspRI CASTG 3 cut(s) 976, 1783, 1807
Tth111I GACNNNGTC 1 cut(s) 284
Vha464I CTTAAG 2 cut(s) 785, 1846
VneI GTGCAC 1 cut(s) 1458
VpaK11BI GGWCC 1 cut(s) 251
XapI RAATTY 3 cut(s) 1096, 1703, 1903
XceI RCATGY 2 cut(s) 2024, 2146
XmiI GTMKAC 1 cut(s) 2122
XmnI GAANNNNTTC 1 cut(s) 2114
XspI CTAG 2 cut(s) 1187, 1415
Zsp2I ATGCAT 1 cut(s) 2148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.