Rw7G042410

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0009318
Physical Location & Seq
Reverse (-)
66044930 .. 66048949
4020 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G042410.1

Sequence Viewer

Length: 2196 bp
ATGGATAATTTTTGTTTGAAAACCACAACGGGGATGCCCGGAATAGCTCCGACAATTACAGCTGCCCTGCCGATCGACGTGCGTACGAACCCGTCTCAGGTGAGCGCGCTGGGGCGGTCCACCGTGGACAGCAATCACAAGAGGTTCTCCTTCAAGTACCCTTTGAAATCTCTCTGGCCCGGAGCCGGAAGAGGAGCCGCTCCTGCGCACAATGGGTTGCCTCTCGACGACGCCATTCCCTTGGCCCAGGAGGAGAAGGAGGACGACGACGACTGCCTTACCCCAACCCAAAGCGGCAACTGGGTTTTAAAGATTTTGCACGTCAGGTCTCTCTGGGGAGGAGGAGGAGCAGATCAACAGGGCAAGGCAGAGCTGCGTGAGGAAGATGAAGCCGCGCCCAACGATAACAGAAATGGTGATGATGACGAAAATGACGACGATTGTGATGGTTGTAGAATTGAACAACAAGACGATCAACAACAACAGCAACAGCAAGAGCAGCAGCAGGTTGAGTTTGATAGAGATTCGTTTTCCAGATTGCTTCGGAAGGTTTCCTTGGCGGAAGCCAGGTTATATGCTCAGATGTCGTATTTGGGGAGCTTGGCTTACTCCATTCCCAAAATCCAGCCACAAAATCTCTTGAGAGGTCATGCTCTGCGGTTTGTTACTTCTTCAATGGAAAAGAAGGAACTTGCTGCTAAAATTGAGAAACATCAAGAGTCGGCTGCAGTTGAAGAAACTGAAAGCACCCTAAAGGAAGACATGGAAGAAGACGAGGAAGGGAAGGAGCAGAAGAACAATGGATACAGAATAAGTCCCTCTGCTGCTTATCATATTGCTGCCTCTGCAGCTTCCTATTTGCATTCCCATACAAGGAGCATACTTCCATTCAAATCCTCAAAGGATGTGACTGATCAAGATTCAACAGAAGAAAGCAGAGGAAGAGGCAGCGCTGTTAATATGATGAACTCAGACATGGCTTCTTTGATGGCAACCACGGACTCAGTAACTTCTGTGGTTGCTGCAAAGGAAGAAGTAAAGCAGGCTGTTGCAGATGATTTAAACTCAACACATTCATCCCCGTGCGACTGGTTCATATGTGATGATGATCAAAGTGCCACCAGATTCTTGGTCATTCAGGGGTCTGAATCTCTGGCATCCTGGCAAGCAAATCTACTTTTCGAGCCCATTCAATTCGAGGGACTGGATGTGCTTGTGCATAGAGGCATATATGAGGCTGCAAAAGGGATTTATGGACAGGTGTTGCCTGAAGTTCATGCACACCTCAAATCTCATGGTGATAAAGCAACCTTCCGTTTTACTGGGCACTCATTAGGGGGAAGCTTAGCGCTACTAATAAACCTCATGCTACTTATACGGGGTGAAGTGCCAGTTTCTTCATTACTTCCTGTTATAACTTTTGGTTCACCAACCATCATGTGTGGAGGTGATCAACTGCTTCGCAAACTTGGATTACCACAGAGTCATATCCAGGCAATTACATTGCACAGAGACATTGTACCCCGGGCATTCTCTTGCAATTATCCTAATCATGTGGCAGAGCTTCTCAAAGCTTTAAGTGGGAACTTCCGGAATCATCCCTGCCTTAATAACCAGAAGCTACTGTATTGTCCAATGGGGGAGCTGTTGATTCTTCAACCAGATGAGAAATTCTCCCCAAACCATCATCTTCTGCCTTCAGGCAGCGGTTTATATGTTCTAAGCGGTGCTCTACCAGATGTCAATGATGCAGAGAAGCAGCTGCGTGCAGCAAAGTTGGTATTCTTAAACTCTCCACATCCACTTGAAATCTTAAGTGATCGGTCTGCATATGGTTCAGAAGGATGCATTCAAAGAGATCATGACATGAACTCTTACTTGAAATGTGTTCGTGGGGTGATTCGACAAGAGCTTAACCATATTAGGAAGGCCAGAAGACAGCAAAGGCGCAAGGTTTGGTGGCCCCTGGTGTCATCCCGGGGTGTGGATGTTGGAATCATTGTGGGAAGGCCTTTTGCGACAATGAAGCAGGGCCAGTACCAGGTCAATTTTTCTGGCGTGTTACAAAGTGGGAAAGAGTCCTTGAAACGGTTCAGTAGACTGGTTGCATCACAGCACATGCATTTGTTTGTGGTGCTTTTGGTACCAGCACGACTCATACTCTTGGGGGCATATAGTGTGATTGGTTTCCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

731

Amino Acids

80.87

Weight (kDa)

6.04

Isoelectric Point (pI)

48.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 377 - 514 1.6e-26 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1415
Acc16I TGCGCA 1 cut(s) 207
Acc36I ACCTGC 1 cut(s) 496
Acc65I GGTACC 1 cut(s) 2143
AccB1I GGYRCC 1 cut(s) 2143
AccBSI CCGCTC 1 cut(s) 200
AccI GTMKAC 1 cut(s) 2098
AccII CGCG 2 cut(s) 107, 395
AccIII TCCGGA 1 cut(s) 1590
AciI CCGC 9 cut(s) 115, 198, 294, 393, 560, 658, 1707, 1725, 2191
AcsI RAATTY 1 cut(s) 1670
AcuI CTGAAG 2 cut(s) 1290, 1683
AcyI GRCGYC 1 cut(s) 231
AfaI GTAC 5 cut(s) 85, 158, 1521, 2039, 2145
AfeI AGCGCT 2 cut(s) 952, 1350
AfiI CCNNNNNNNGG 7 cut(s) 30, 97, 185, 873, 1984, 2041, 2088
AflII CTTAAG 1 cut(s) 1813
AjiI CACGTC 2 cut(s) 79, 322
AjnI CCWGG 6 cut(s) 246, 566, 1160, 1491, 1965, 2040
AjuI GAANNNNNNNTTGG 2 cut(s) 539, 571
Alw21I GWGCWC 1 cut(s) 1732
Alw26I GTCTC 3 cut(s) 99, 333, 1506
Ama87I CYCGRG 2 cut(s) 1524, 1977
Aor13HI TCCGGA 1 cut(s) 1590
Aor51HI AGCGCT 2 cut(s) 952, 1350
AoxI GGCC 6 cut(s) 176, 243, 1929, 1961, 2009, 2032
ApoI RAATTY 1 cut(s) 1670
Asp700I GAANNNNTTC 1 cut(s) 2090
Asp718I GGTACC 1 cut(s) 2143
AspLEI GCGC 7 cut(s) 107, 109, 208, 397, 953, 1351, 1950
AspS9I GGNCC 5 cut(s) 117, 177, 244, 1962, 2032
AsuC2I CCSGG 6 cut(s) 39, 180, 1525, 1526, 1978, 1979
AsuHPI GGTGA 7 cut(s) 112, 428, 1310, 1394, 1419, 1460, 1909
AvaI CYCGRG 2 cut(s) 1524, 1977
AvaII GGWCC 1 cut(s) 117
BaeGI GKGCMC 1 cut(s) 1329
BanI GGYRCC 1 cut(s) 2143
BanII GRGCYC 1 cut(s) 1188
BbsI GAAGAC 3 cut(s) 765, 777, 1942
Bbv12I GWGCWC 1 cut(s) 1732
BccI CCATC 4 cut(s) 440, 982, 1442, 1692
BcgI CGANNNNNNTGC 2 cut(s) 61, 95
BciT130I CCWGG 6 cut(s) 248, 568, 1162, 1493, 1967, 2042
BciVI GTATCC 1 cut(s) 797
BclI TGATCA 3 cut(s) 913, 1108, 1450
BcnI CCSGG 6 cut(s) 39, 180, 1525, 1526, 1978, 1979
BcoDI GTCTC 3 cut(s) 99, 333, 1506
BfmI CTRYAG 2 cut(s) 726, 846
BfoI RGCGCY 2 cut(s) 954, 1352
BfrI CTTAAG 1 cut(s) 1813
BfuAI ACCTGC 1 cut(s) 496
BfuI GTATCC 1 cut(s) 797
BlpI GCTNAGC 1 cut(s) 1345
Bme18I GGWCC 1 cut(s) 117
BmeT110I CYCGRG 2 cut(s) 1524, 1977
BmgBI CACGTC 2 cut(s) 79, 322
BmgT120I GGNCC 5 cut(s) 117, 177, 244, 1962, 2032
BmiI GGNNCC 4 cut(s) 184, 196, 1964, 2145
BmrI ACTGGG 2 cut(s) 310, 1332
BmsI GCATC 5 cut(s) 24, 1166, 1738, 1835, 2117
BmuI ACTGGG 2 cut(s) 310, 1332
BpiI GAAGAC 3 cut(s) 765, 777, 1942
BplI GAGNNNNNCTC 2 cut(s) 1658, 1690
Bpu1102I GCTNAGC 1 cut(s) 1345
BpuEI CTTGAG 1 cut(s) 661
BpuMI CCSGG 6 cut(s) 39, 180, 1525, 1526, 1978, 1979
BsaBI GATNNNNATC 1 cut(s) 1107
BsaHI GRCGYC 1 cut(s) 231
BsaI GGTCTC 1 cut(s) 333
BsaWI WCCGGW 1 cut(s) 1590
Bsc4I CCNNNNNNNGG 7 cut(s) 30, 97, 185, 873, 1984, 2041, 2088
Bse1I ACTGG 7 cut(s) 305, 1094, 1209, 1327, 1391, 2035, 2106
Bse3DI GCAATG 1 cut(s) 1502
Bse8I GATNNNNATC 1 cut(s) 1107
BseAI TCCGGA 1 cut(s) 1590
BseBI CCWGG 6 cut(s) 248, 568, 1162, 1493, 1967, 2042
BseJI GATNNNNATC 1 cut(s) 1107
BseLI CCNNNNNNNGG 7 cut(s) 30, 97, 185, 873, 1984, 2041, 2088
BseMI GCAATG 1 cut(s) 1502
BseMII CTCAG 4 cut(s) 110, 593, 984, 1017
BseNI ACTGG 7 cut(s) 305, 1094, 1209, 1327, 1391, 2035, 2106
BsePI GCGCGC 1 cut(s) 105
BseRI GAGGAG 5 cut(s) 207, 266, 354, 357, 360
BseSI GKGCMC 1 cut(s) 1329
BseYI CCCAGC 1 cut(s) 109
BsgI GTGCAG 1 cut(s) 1788
Bsh1236I CGCG 2 cut(s) 107, 395
Bsh1285I CGRYCG 1 cut(s) 75
BshFI GGCC 6 cut(s) 178, 245, 1931, 1963, 2011, 2034
BshNI GGYRCC 1 cut(s) 2143
BsiEI CGRYCG 1 cut(s) 75
BsiHKAI GWGCWC 1 cut(s) 1732
BsiHKCI CYCGRG 2 cut(s) 1524, 1977
BsiSI CCGG 6 cut(s) 39, 180, 186, 1525, 1591, 1978
BsiWI CGTACG 1 cut(s) 83
BslFI GGGAC 2 cut(s) 801, 1215
BslI CCNNNNNNNGG 7 cut(s) 30, 97, 185, 873, 1984, 2041, 2088
BsmAI GTCTC 3 cut(s) 99, 333, 1506
BsmBI CGTCTC 1 cut(s) 99
BsmFI GGGAC 2 cut(s) 801, 1215
BsmI GAATGC 3 cut(s) 862, 1529, 1848
BsnI GGCC 6 cut(s) 178, 245, 1931, 1963, 2011, 2034
Bso31I GGTCTC 1 cut(s) 333
BsoBI CYCGRG 2 cut(s) 1524, 1977
Bsp1286I GDGCHC 3 cut(s) 1188, 1329, 1732
Bsp13I TCCGGA 1 cut(s) 1590
Bsp143I GATC 8 cut(s) 72, 352, 472, 913, 1108, 1450, 1819, 1858
Bsp1720I GCTNAGC 1 cut(s) 1345
BspACI CCGC 9 cut(s) 115, 198, 294, 393, 560, 658, 1707, 1725, 2191
BspANI GGCC 6 cut(s) 178, 245, 1931, 1963, 2011, 2034
BspCNI CTCAG 4 cut(s) 109, 592, 983, 1016
BspEI TCCGGA 1 cut(s) 1590
BspFNI CGCG 2 cut(s) 107, 395
BspHI TCATGA 1 cut(s) 1861
BspLI GGNNCC 4 cut(s) 184, 196, 1964, 2145
BspMAI CTGCAG 2 cut(s) 730, 850
BspMI ACCTGC 1 cut(s) 496
BspT107I GGYRCC 1 cut(s) 2143
BspTI CTTAAG 1 cut(s) 1813
BspTNI GGTCTC 1 cut(s) 333
BsrBI CCGCTC 1 cut(s) 200
BsrDI GCAATG 1 cut(s) 1502
BsrI ACTGG 7 cut(s) 305, 1094, 1209, 1327, 1391, 2035, 2106
BssHII GCGCGC 1 cut(s) 105
BssMI GATC 8 cut(s) 72, 352, 472, 913, 1108, 1450, 1819, 1858
BssNI GRCGYC 1 cut(s) 231
BssT1I CCWWGG 2 cut(s) 240, 555
Bst2UI CCWGG 6 cut(s) 248, 568, 1162, 1493, 1967, 2042
Bst4CI ACNGT 3 cut(s) 124, 1626, 2091
Bst6I CTCTTC 2 cut(s) 184, 937
BstACI GRCGYC 1 cut(s) 231
BstAFI CTTAAG 1 cut(s) 1813
BstC8I GCNNGC 4 cut(s) 107, 1044, 1167, 1767
BstDEI CTNAG 6 cut(s) 96, 579, 970, 1003, 1345, 1721
BstDSI CCRYGG 2 cut(s) 123, 996
BstFNI CGCG 2 cut(s) 107, 395
BstH2I RGCGCY 2 cut(s) 954, 1352
BstHHI GCGC 7 cut(s) 107, 109, 208, 397, 953, 1351, 1950
BstKTI GATC 8 cut(s) 75, 355, 475, 916, 1111, 1453, 1822, 1861
BstMAI GTCTC 3 cut(s) 99, 333, 1506
BstMBI GATC 8 cut(s) 72, 352, 472, 913, 1108, 1450, 1819, 1858
BstMCI CGRYCG 1 cut(s) 75
BstMWI GCNNNNNNNGC 4 cut(s) 203, 499, 845, 848
BstNI CCWGG 6 cut(s) 248, 568, 1162, 1493, 1967, 2042
BstNSI RCATGY 1 cut(s) 2122
BstSFI CTRYAG 2 cut(s) 726, 846
BstSLI GKGCMC 1 cut(s) 1329
BstUI CGCG 2 cut(s) 107, 395
BstV2I GAAGAC 3 cut(s) 765, 777, 1942
BstXI CCANNNNNNTGG 2 cut(s) 241, 1129
BsuI GTATCC 1 cut(s) 797
BsuRI GGCC 6 cut(s) 178, 245, 1931, 1963, 2011, 2034
BtgI CCRYGG 2 cut(s) 123, 996
BtrI CACGTC 2 cut(s) 79, 322
BveI ACCTGC 1 cut(s) 496
Cac8I GCNNGC 4 cut(s) 107, 1044, 1167, 1767
CciI TCATGA 1 cut(s) 1861
CfoI GCGC 7 cut(s) 107, 109, 208, 397, 953, 1351, 1950
Cfr13I GGNCC 5 cut(s) 117, 177, 244, 1962, 2032
Cfr9I CCCGGG 2 cut(s) 1524, 1977
CseI GACGC 1 cut(s) 239
CsiI ACCWGGT 1 cut(s) 2040
Csp6I GTAC 5 cut(s) 84, 157, 1520, 2038, 2144
CviQI GTAC 5 cut(s) 84, 157, 1520, 2038, 2144
DdeI CTNAG 6 cut(s) 96, 579, 970, 1003, 1345, 1721
DpnI GATC 8 cut(s) 74, 354, 474, 915, 1110, 1452, 1821, 1860
DpnII GATC 8 cut(s) 72, 352, 472, 913, 1108, 1450, 1819, 1858
DraI TTTAAA 2 cut(s) 309, 1062
Eam1104I CTCTTC 2 cut(s) 184, 937
EarI CTCTTC 2 cut(s) 184, 937
EciI GGCGGA 1 cut(s) 575
Eco130I CCWWGG 2 cut(s) 240, 555
Eco147I AGGCCT 1 cut(s) 2011
Eco24I GRGCYC 1 cut(s) 1188
Eco31I GGTCTC 1 cut(s) 333
Eco47I GGWCC 1 cut(s) 117
Eco47III AGCGCT 2 cut(s) 952, 1350
Eco57I CTGAAG 2 cut(s) 1290, 1683
Eco88I CYCGRG 2 cut(s) 1524, 1977
EcoRII CCWGG 6 cut(s) 246, 566, 1160, 1491, 1965, 2040
EcoT14I CCWWGG 2 cut(s) 240, 555
EcoT22I ATGCAT 2 cut(s) 1850, 2124
EcoT38I GRGCYC 1 cut(s) 1188
ErhI CCWWGG 2 cut(s) 240, 555
Esp3I CGTCTC 1 cut(s) 99
FalI AAGNNNNNCTT 2 cut(s) 539, 571
FaqI GGGAC 2 cut(s) 801, 1215
FauNDI CATATG 2 cut(s) 1097, 1831
FbaI TGATCA 3 cut(s) 913, 1108, 1450
FblI GTMKAC 1 cut(s) 2098
FriOI GRGCYC 1 cut(s) 1188
FspI TGCGCA 1 cut(s) 207
GlaI GCGC 7 cut(s) 106, 108, 207, 396, 952, 1350, 1949
GsaI CCCAGC 1 cut(s) 113
HaeII RGCGCY 2 cut(s) 954, 1352
HaeIII GGCC 6 cut(s) 178, 245, 1931, 1963, 2011, 2034
HapII CCGG 6 cut(s) 39, 180, 186, 1525, 1591, 1978
HgaI GACGC 1 cut(s) 239
HhaI GCGC 7 cut(s) 107, 109, 208, 397, 953, 1351, 1950
Hin1I GRCGYC 1 cut(s) 231
Hin6I GCGC 7 cut(s) 105, 107, 206, 395, 951, 1349, 1948
HinP1I GCGC 7 cut(s) 105, 107, 206, 395, 951, 1349, 1948
HindIII AAGCTT 2 cut(s) 1342, 1572
HpaII CCGG 6 cut(s) 39, 180, 186, 1525, 1591, 1978
HphI GGTGA 7 cut(s) 112, 428, 1310, 1394, 1419, 1460, 1909
Hpy166II GTNNAC 4 cut(s) 120, 127, 1427, 2099
Hpy188I TCNGA 6 cut(s) 51, 546, 582, 973, 1147, 1840
Hpy188III TCNNGA 7 cut(s) 224, 534, 640, 716, 917, 1591, 1862
Hpy8I GTNNAC 4 cut(s) 120, 127, 1427, 2099
Hpy99I CGWCG 6 cut(s) 80, 230, 233, 269, 272, 440
HpyCH4III ACNGT 3 cut(s) 124, 1626, 2091
HpyCH4IV ACGT 2 cut(s) 78, 321
HpyF10VI GCNNNNNNNGC 4 cut(s) 203, 499, 845, 848
HpyF3I CTNAG 6 cut(s) 96, 579, 970, 1003, 1345, 1721
HpySE526I ACGT 2 cut(s) 78, 321
Hsp92I GRCGYC 1 cut(s) 231
HspAI GCGC 7 cut(s) 105, 107, 206, 395, 951, 1349, 1948
Kpn2I TCCGGA 1 cut(s) 1590
KpnI GGTACC 1 cut(s) 2147
Ksp22I TGATCA 3 cut(s) 913, 1108, 1450
Kzo9I GATC 8 cut(s) 72, 352, 472, 913, 1108, 1450, 1819, 1858
LmnI GCTCC 9 cut(s) 52, 182, 194, 205, 347, 597, 787, 876, 1642
LweI GCATC 5 cut(s) 24, 1166, 1738, 1835, 2117
MabI ACCWGGT 1 cut(s) 2040
MaeII ACGT 2 cut(s) 78, 321
MaeIII GTNAC 4 cut(s) 664, 907, 1006, 2061
MalI GATC 8 cut(s) 74, 354, 474, 915, 1110, 1452, 1821, 1860
MbiI CCGCTC 1 cut(s) 200
MboI GATC 8 cut(s) 72, 352, 472, 913, 1108, 1450, 1819, 1858
MhlI GDGCHC 3 cut(s) 1188, 1329, 1732
MluCI AATT 9 cut(s) 7, 54, 456, 702, 1193, 1497, 1540, 1670, 2047
MlyI GAGTC 5 cut(s) 728, 995, 1492, 2087, 2148
MmeI TCCRAC 2 cut(s) 74, 1972
Mph1103I ATGCAT 2 cut(s) 1850, 2124
MroI TCCGGA 1 cut(s) 1590
MroXI GAANNNNTTC 1 cut(s) 2090
MseI TTAA 8 cut(s) 308, 957, 1061, 1577, 1608, 1787, 1814, 1914
MslI CAYNNNNRTG 1 cut(s) 1081
MspA1I CMGCKG 4 cut(s) 62, 1707, 1762, 2193
MspCI CTTAAG 1 cut(s) 1813
MspI CCGG 6 cut(s) 39, 180, 186, 1525, 1591, 1978
Mva1269I GAATGC 3 cut(s) 862, 1529, 1848
MvaI CCWGG 6 cut(s) 248, 568, 1162, 1493, 1967, 2042
MvnI CGCG 2 cut(s) 107, 395
MwoI GCNNNNNNNGC 4 cut(s) 203, 499, 845, 848
NciI CCSGG 6 cut(s) 39, 180, 1525, 1526, 1978, 1979
NdeI CATATG 2 cut(s) 1097, 1831
NdeII GATC 8 cut(s) 72, 352, 472, 913, 1108, 1450, 1819, 1858
NlaIV GGNNCC 4 cut(s) 184, 196, 1964, 2145
NmuCI GTSAC 1 cut(s) 907
NsbI TGCGCA 1 cut(s) 207
NsiI ATGCAT 2 cut(s) 1850, 2124
NspI RCATGY 1 cut(s) 2122
PagI TCATGA 1 cut(s) 1861
PauI GCGCGC 1 cut(s) 105
PceI AGGCCT 1 cut(s) 2011
PcsI WCGNNNNNNNCGW 1 cut(s) 432
PctI GAATGC 3 cut(s) 862, 1529, 1848
PdmI GAANNNNTTC 1 cut(s) 2090
PfeI GAWTC 8 cut(s) 524, 920, 1125, 1148, 1594, 1651, 1900, 1995
Pfl23II CGTACG 1 cut(s) 83
Ple19I CGATCG 1 cut(s) 75
PleI GAGTC 5 cut(s) 727, 995, 1491, 2086, 2148
PpsI GAGTC 5 cut(s) 727, 995, 1491, 2086, 2148
PsiI TTATAA 1 cut(s) 1415
Psp6I CCWGG 6 cut(s) 246, 566, 1160, 1491, 1965, 2040
PspFI CCCAGC 1 cut(s) 109
PspGI CCWGG 6 cut(s) 246, 566, 1160, 1491, 1965, 2040
PspLI CGTACG 1 cut(s) 83
PspN4I GGNNCC 4 cut(s) 184, 196, 1964, 2145
PspPI GGNCC 5 cut(s) 117, 177, 244, 1962, 2032
PsrI GAACNNNNNNTAC 2 cut(s) 788, 820
PstI CTGCAG 2 cut(s) 730, 850
PteI GCGCGC 1 cut(s) 105
PvuI CGATCG 1 cut(s) 75
PvuII CAGCTG 2 cut(s) 62, 1762
RsaI GTAC 5 cut(s) 85, 158, 1521, 2039, 2145
RsaNI GTAC 5 cut(s) 84, 157, 1520, 2038, 2144
RseI CAYNNNNRTG 1 cut(s) 1081
SaqAI TTAA 8 cut(s) 308, 957, 1061, 1577, 1608, 1787, 1814, 1914
Sau3AI GATC 8 cut(s) 72, 352, 472, 913, 1108, 1450, 1819, 1858
Sau96I GGNCC 5 cut(s) 117, 177, 244, 1962, 2032
SchI GAGTC 5 cut(s) 728, 995, 1492, 2087, 2148
SduI GDGCHC 3 cut(s) 1188, 1329, 1732
SexAI ACCWGGT 1 cut(s) 2040
SfaNI GCATC 5 cut(s) 24, 1166, 1738, 1835, 2117
SfcI CTRYAG 2 cut(s) 726, 846
SinI GGWCC 1 cut(s) 117
SmaI CCCGGG 2 cut(s) 1526, 1979
SmiMI CAYNNNNRTG 1 cut(s) 1081
SmlI CTYRAG 2 cut(s) 640, 1813
SmoI CTYRAG 2 cut(s) 640, 1813
Sse9I AATT 9 cut(s) 7, 54, 456, 702, 1193, 1497, 1540, 1670, 2047
SseBI AGGCCT 1 cut(s) 2011
SsiI CCGC 9 cut(s) 115, 198, 294, 393, 560, 658, 1707, 1725, 2191
StuI AGGCCT 1 cut(s) 2011
StyI CCWWGG 2 cut(s) 240, 555
TaaI ACNGT 3 cut(s) 124, 1626, 2091
TaiI ACGT 2 cut(s) 81, 324
TaqI TCGA 5 cut(s) 75, 225, 1182, 1197, 1903
TaqII GACCGA 1 cut(s) 1812
TasI AATT 9 cut(s) 7, 54, 456, 702, 1193, 1497, 1540, 1670, 2047
TauI GCSGC 3 cut(s) 200, 297, 395
TfiI GAWTC 8 cut(s) 524, 920, 1125, 1148, 1594, 1651, 1900, 1995
Tru1I TTAA 8 cut(s) 308, 957, 1061, 1577, 1608, 1787, 1814, 1914
Tru9I TTAA 8 cut(s) 308, 957, 1061, 1577, 1608, 1787, 1814, 1914
TseFI GTSAC 1 cut(s) 907
Tsp45I GTSAC 1 cut(s) 907
TspDTI ATGAA 8 cut(s) 402, 980, 1065, 1084, 1265, 1389, 1883, 2039
TspGWI ACGGA 2 cut(s) 1013, 1304
TspMI CCCGGG 2 cut(s) 1524, 1977
Vha464I CTTAAG 1 cut(s) 1813
VpaK11BI GGWCC 1 cut(s) 117
XapI RAATTY 1 cut(s) 1670
XceI RCATGY 1 cut(s) 2122
XcmI CCANNNNNNNNNTGG 1 cut(s) 1126
XmaI CCCGGG 2 cut(s) 1524, 1977
XmiI GTMKAC 1 cut(s) 2098
XmnI GAANNNNTTC 1 cut(s) 2090
Zsp2I ATGCAT 2 cut(s) 1850, 2124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.