pycom03g23540

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0009318
Physical Location & Seq
Reverse (-)
23331102 .. 23332815
1714 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g23540.1

Sequence Viewer

Length: 1236 bp
ATGAACTCAGAAGTGGCTTCTTTAATGGCAACCACCGACTCAGTTACAGCCGTGGTTGCTGCAAAGGAAGAAGTAAAGCAGGCTGTTGCAGATGACTTAAATTCAACACTTTCATCACCTTGTGAATGGTTCGTATGCGATGATGATCAAAGCGGCACCAGATTCTTTGTCATTCAGGGGTCTGACTCCTTAGCATCCTGGCAAGCAAATCTACTTTTTGAGCCCATTCAGTTTGAGGGACTGGATGTGCTTGTGCATAGAGGCATATACGAGGCTGCGAAAGGGATTTACGAACAGATGCTGCCTGAAGTCCATGCCCACCTCAAATCTTGTGGTGACCGTGCAACCTTTCGTTTTACTGGGCATTCTTTAGGGGGAAGCTTGGCACTACTAATAAATCTCATGTTGCTTATACGGTTAGAAGTGCCAGTTTCTTCACTGCTTCCTGTTATTACTTTTGGTGCACCATCCATTATGTGTGGAGGTGACCAATTGCTTCGCAAGCTTGGATTACTACGAAATCATGTTCAGTCTATTACGTTGCACAGAGACATTGTACCCCGTGCCTTCTCTTGCAATTACCCAAATCATGTGGCAGAGCTTCTCAAGGCTTTGAATGGGAACTTTCGGAATCATCCCTGTCTCAATAACCAGAAGCTACTGTATTCTCCAATGGGGGAGCTCCTGATTCTTCAACCAGATGAGAAATTCTCCCCAAATCATCATCTTCTTCCATCAGGCAGCGGTCTATATCTTCTAAGCTGTCCGCTGTCTGATGGCAGTGATGCAGAGAGGCAGCTCCTCGCTGCACAGTTGGTATTCCTAAACTCACCACACCCACTCGAGATCCTAAGCGATCGGTCTGCATATGGTTCAGAAGGAACCATTCAAAGAGATCATGATGTGAATTCTTACTTAAAATCTGTTCGTGGGGTAATTCGGCAAGAGCTTAACCAGATTAGGAAGTCTAGGAGACAGCAGCGCCGCAAGGTGTGGTGGCCCTTGATAGCACCCCGGAGCATGCATGCAGGAGGTGTGATTGTGGGAAGGACAATTGCCTCAATCAACCTGGGAAAAGAGCAATTAAACTTTTCTGGCGTATTACGGACGGGGAGAGAGTCGTTGAAACGGTTCAGTAGACTTGTTGCATCGCGGCACATGCATTTGTTTGTGGTGCTTTTGGTACCAGCGCGATTGTTAGTCTTGGGGGCATACAGCATCATCGGTTTCCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

412

Amino Acids

45.57

Weight (kDa)

8.95

Isoelectric Point (pI)

42.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 56 - 193 2.3e-26 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1183
AccB1I GGYRCC 2 cut(s) 155, 1183
AccI GTMKAC 1 cut(s) 1138
AccII CGCG 2 cut(s) 1153, 1192
AciI CCGC 5 cut(s) 153, 744, 767, 985, 1153
AclWI GGATC 1 cut(s) 841
AcsI RAATTY 3 cut(s) 100, 707, 907
AcuI CTGAAG 1 cut(s) 327
AdeI CACNNNGTG 1 cut(s) 122
AfaI GTAC 2 cut(s) 558, 1185
AgsI TTSAA 5 cut(s) 105, 616, 695, 890, 1126
AjnI CCWGG 2 cut(s) 197, 1068
AluBI AGCT 8 cut(s) 381, 505, 601, 658, 682, 762, 799, 949
AluI AGCT 8 cut(s) 381, 505, 601, 658, 682, 762, 799, 949
Alw21I GWGCWC 2 cut(s) 466, 684
Alw26I GTCTC 3 cut(s) 543, 647, 967
Alw44I GTGCAC 1 cut(s) 462
AlwI GGATC 1 cut(s) 841
AlwNI CAGNNNCTG 1 cut(s) 301
Ama87I CYCGRG 1 cut(s) 842
AoxI GGCC 1 cut(s) 998
ApaLI GTGCAC 1 cut(s) 462
ApeKI GCWGC 7 cut(s) 59, 275, 301, 741, 796, 806, 979
ApoI RAATTY 3 cut(s) 100, 707, 907
Asp700I GAANNNNTTC 1 cut(s) 1130
Asp718I GGTACC 1 cut(s) 1183
AspLEI GCGC 2 cut(s) 984, 1192
AspS9I GGNCC 1 cut(s) 999
AsuC2I CCSGG 1 cut(s) 1015
AsuHPI GGTGA 4 cut(s) 108, 347, 497, 822
AvaI CYCGRG 1 cut(s) 842
BaeGI GKGCMC 1 cut(s) 466
BanI GGYRCC 2 cut(s) 155, 1183
BanII GRGCYC 2 cut(s) 225, 684
Bbv12I GWGCWC 2 cut(s) 466, 684
BbvI GCAGC 7 cut(s) 46, 262, 288, 753, 793, 808, 991
BccI CCATC 3 cut(s) 475, 742, 770
BceAI ACGGC 1 cut(s) 35
BcgI CGANNNNNNTGC 2 cut(s) 845, 879
BciT130I CCWGG 2 cut(s) 199, 1070
BclI TGATCA 1 cut(s) 145
BcnI CCSGG 1 cut(s) 1015
BcoDI GTCTC 3 cut(s) 543, 647, 967
BfaI CTAG 1 cut(s) 969
BfoI RGCGCY 1 cut(s) 985
Bme1390I CCNGG 3 cut(s) 199, 1015, 1070
BmeT110I CYCGRG 1 cut(s) 842
BmgT120I GGNCC 1 cut(s) 999
BmiI GGNNCC 3 cut(s) 157, 883, 1185
BmrFI CCNGG 3 cut(s) 199, 1015, 1070
BmrI ACTGGG 1 cut(s) 369
BmsI GCATC 5 cut(s) 203, 288, 775, 1157, 1227
BmuI ACTGGG 1 cut(s) 369
BplI GAGNNNNNCTC 2 cut(s) 695, 727
Bpu10I CCTNAGC 2 cut(s) 190, 851
BpuEI CTTGAG 1 cut(s) 590
BpuMI CCSGG 1 cut(s) 1015
BsaBI GATNNNNATC 1 cut(s) 144
BsaJI CCNNGG 3 cut(s) 51, 1013, 1069
BsaWI WCCGGW 1 cut(s) 1230
Bse1I ACTGG 3 cut(s) 246, 364, 428
Bse8I GATNNNNATC 1 cut(s) 144
BseBI CCWGG 2 cut(s) 199, 1070
BseDI CCNNGG 3 cut(s) 51, 1013, 1069
BseGI GGATG 4 cut(s) 194, 250, 467, 634
BseJI GATNNNNATC 1 cut(s) 144
BseMII CTCAG 2 cut(s) 21, 54
BseNI ACTGG 3 cut(s) 246, 364, 428
BseRI GAGGAG 1 cut(s) 791
BseSI GKGCMC 1 cut(s) 466
BseXI GCAGC 7 cut(s) 46, 262, 288, 753, 793, 808, 991
BsgI GTGCAG 1 cut(s) 792
Bsh1236I CGCG 2 cut(s) 1153, 1192
Bsh1285I CGRYCG 1 cut(s) 859
BshFI GGCC 1 cut(s) 1000
BshNI GGYRCC 2 cut(s) 155, 1183
BsiEI CGRYCG 1 cut(s) 859
BsiHKAI GWGCWC 2 cut(s) 466, 684
BsiHKCI CYCGRG 1 cut(s) 842
BsiSI CCGG 2 cut(s) 1015, 1231
BslFI GGGAC 1 cut(s) 252
BsmAI GTCTC 3 cut(s) 543, 647, 967
BsmFI GGGAC 1 cut(s) 252
BsmI GAATGC 1 cut(s) 364
BsnI GGCC 1 cut(s) 1000
BsoBI CYCGRG 1 cut(s) 842
Bsp1286I GDGCHC 3 cut(s) 225, 466, 684
Bsp143I GATC 4 cut(s) 145, 846, 856, 895
BspACI CCGC 5 cut(s) 153, 744, 767, 985, 1153
BspANI GGCC 1 cut(s) 1000
BspCNI CTCAG 2 cut(s) 20, 53
BspFNI CGCG 2 cut(s) 1153, 1192
BspHI TCATGA 1 cut(s) 898
BspLI GGNNCC 3 cut(s) 157, 883, 1185
BspPI GGATC 1 cut(s) 841
BspT107I GGYRCC 2 cut(s) 155, 1183
BsrI ACTGG 3 cut(s) 246, 364, 428
BssECI CCNNGG 3 cut(s) 51, 1013, 1069
BssMI GATC 4 cut(s) 145, 846, 856, 895
Bst2UI CCWGG 2 cut(s) 199, 1070
Bst4CI ACNGT 5 cut(s) 341, 417, 663, 813, 1131
BstC8I GCNNGC 5 cut(s) 81, 204, 503, 1022, 1026
BstDEI CTNAG 5 cut(s) 7, 40, 190, 758, 851
BstDSI CCRYGG 1 cut(s) 51
BstEII GGTNACC 2 cut(s) 335, 485
BstF5I GGATG 4 cut(s) 194, 250, 467, 634
BstFNI CGCG 2 cut(s) 1153, 1192
BstH2I RGCGCY 1 cut(s) 985
BstHHI GCGC 2 cut(s) 984, 1192
BstKTI GATC 4 cut(s) 148, 849, 859, 898
BstMAI GTCTC 3 cut(s) 543, 647, 967
BstMBI GATC 4 cut(s) 145, 846, 856, 895
BstMCI CGRYCG 1 cut(s) 859
BstMWI GCNNNNNNNGC 3 cut(s) 56, 502, 1159
BstNI CCWGG 2 cut(s) 199, 1070
BstNSI RCATGY 3 cut(s) 1024, 1028, 1162
BstPI GGTNACC 2 cut(s) 335, 485
BstSCI CCNGG 3 cut(s) 197, 1013, 1068
BstSLI GKGCMC 1 cut(s) 466
BstUI CGCG 2 cut(s) 1153, 1192
BstV1I GCAGC 7 cut(s) 46, 262, 288, 753, 793, 808, 991
BstX2I RGATCY 1 cut(s) 846
BstYI RGATCY 1 cut(s) 846
BsuRI GGCC 1 cut(s) 1000
BtgI CCRYGG 1 cut(s) 51
BtgZI GCGATG 2 cut(s) 153, 1134
BtsCI GGATG 4 cut(s) 194, 250, 467, 634
BtsI GCAGTG 2 cut(s) 437, 787
BtsIMutI CAGTG 2 cut(s) 437, 787
Cac8I GCNNGC 5 cut(s) 81, 204, 503, 1022, 1026
CaiI CAGNNNCTG 1 cut(s) 301
CciI TCATGA 1 cut(s) 898
CfoI GCGC 2 cut(s) 984, 1192
Cfr13I GGNCC 1 cut(s) 999
Csp6I GTAC 2 cut(s) 557, 1184
CspCI CAANNNNNGTGG 4 cut(s) 313, 348, 573, 608
CviAII CATG 8 cut(s) 314, 403, 524, 590, 899, 1021, 1025, 1159
CviQI GTAC 2 cut(s) 557, 1184
DdeI CTNAG 5 cut(s) 7, 40, 190, 758, 851
DpnI GATC 4 cut(s) 147, 848, 858, 897
DpnII GATC 4 cut(s) 145, 846, 856, 895
DraIII CACNNNGTG 1 cut(s) 122
Ecl136II GAGCTC 1 cut(s) 682
Eco24I GRGCYC 2 cut(s) 225, 684
Eco53kI GAGCTC 1 cut(s) 682
Eco57I CTGAAG 1 cut(s) 327
Eco88I CYCGRG 1 cut(s) 842
Eco91I GGTNACC 2 cut(s) 335, 485
EcoICRI GAGCTC 1 cut(s) 682
EcoO65I GGTNACC 2 cut(s) 335, 485
EcoRI GAATTC 1 cut(s) 907
EcoRII CCWGG 2 cut(s) 197, 1068
EcoT22I ATGCAT 2 cut(s) 1026, 1164
EcoT38I GRGCYC 2 cut(s) 225, 684
FaeI CATG 8 cut(s) 317, 406, 527, 593, 902, 1024, 1028, 1162
FaqI GGGAC 1 cut(s) 252
FatI CATG 8 cut(s) 313, 402, 523, 589, 898, 1020, 1024, 1158
FauNDI CATATG 1 cut(s) 868
FbaI TGATCA 1 cut(s) 145
FblI GTMKAC 1 cut(s) 1138
FokI GGATG 4 cut(s) 181, 257, 454, 621
FriOI GRGCYC 2 cut(s) 225, 684
FspBI CTAG 1 cut(s) 969
GlaI GCGC 2 cut(s) 983, 1191
HaeII RGCGCY 1 cut(s) 985
HaeIII GGCC 1 cut(s) 1000
HapII CCGG 2 cut(s) 1015, 1231
HhaI GCGC 2 cut(s) 984, 1192
Hin1II CATG 8 cut(s) 317, 406, 527, 593, 902, 1024, 1028, 1162
Hin6I GCGC 2 cut(s) 982, 1190
HinP1I GCGC 2 cut(s) 982, 1190
HindIII AAGCTT 2 cut(s) 379, 503
HinfI GANTC 6 cut(s) 38, 162, 185, 631, 688, 1118
HpaII CCGG 2 cut(s) 1015, 1231
HphI GGTGA 4 cut(s) 108, 347, 497, 822
Hpy166II GTNNAC 2 cut(s) 464, 1139
Hpy188I TCNGA 5 cut(s) 10, 184, 630, 775, 877
Hpy188III TCNNGA 3 cut(s) 685, 844, 899
Hpy8I GTNNAC 2 cut(s) 464, 1139
HpyAV CCTTC 3 cut(s) 577, 872, 1041
HpyCH4III ACNGT 5 cut(s) 341, 417, 663, 813, 1131
HpyCH4IV ACGT 1 cut(s) 539
HpyF10VI GCNNNNNNNGC 3 cut(s) 56, 502, 1159
HpyF3I CTNAG 5 cut(s) 7, 40, 190, 758, 851
HpySE526I ACGT 1 cut(s) 539
Hsp92II CATG 8 cut(s) 317, 406, 527, 593, 902, 1024, 1028, 1162
HspAI GCGC 2 cut(s) 982, 1190
KpnI GGTACC 1 cut(s) 1187
Ksp22I TGATCA 1 cut(s) 145
Kzo9I GATC 4 cut(s) 145, 846, 856, 895
LmnI GCTCC 4 cut(s) 679, 687, 804, 1017
Lsp1109I GCAGC 7 cut(s) 46, 262, 288, 753, 793, 808, 991
LweI GCATC 5 cut(s) 203, 288, 775, 1157, 1227
MaeI CTAG 1 cut(s) 969
MaeII ACGT 1 cut(s) 539
MaeIII GTNAC 3 cut(s) 43, 335, 485
MalI GATC 4 cut(s) 147, 848, 858, 897
MboI GATC 4 cut(s) 145, 846, 856, 895
MboII GAAGA 6 cut(s) 80, 426, 683, 719, 722, 746
MfeI CAATTG 2 cut(s) 491, 1053
MflI RGATCY 1 cut(s) 846
MhlI GDGCHC 3 cut(s) 225, 466, 684
MluCI AATT 8 cut(s) 100, 491, 577, 707, 907, 936, 1053, 1082
MlyI GAGTC 3 cut(s) 32, 179, 1127
MnlI CCTC 9 cut(s) 229, 254, 265, 332, 476, 786, 812, 1025, 1069
Mph1103I ATGCAT 2 cut(s) 1026, 1164
MroXI GAANNNNTTC 1 cut(s) 1130
MseI TTAA 5 cut(s) 23, 98, 917, 951, 1085
MspA1I CMGCKG 2 cut(s) 744, 769
MspI CCGG 2 cut(s) 1015, 1231
MspR9I CCNGG 3 cut(s) 199, 1015, 1070
MunI CAATTG 2 cut(s) 491, 1053
Mva1269I GAATGC 1 cut(s) 364
MvaI CCWGG 2 cut(s) 199, 1070
MvnI CGCG 2 cut(s) 1153, 1192
MwoI GCNNNNNNNGC 3 cut(s) 56, 502, 1159
NciI CCSGG 1 cut(s) 1015
NdeI CATATG 1 cut(s) 868
NdeII GATC 4 cut(s) 145, 846, 856, 895
NlaIII CATG 8 cut(s) 317, 406, 527, 593, 902, 1024, 1028, 1162
NlaIV GGNNCC 3 cut(s) 157, 883, 1185
NmuCI GTSAC 2 cut(s) 335, 485
NsiI ATGCAT 2 cut(s) 1026, 1164
NspI RCATGY 3 cut(s) 1024, 1028, 1162
PaeI GCATGC 2 cut(s) 1024, 1028
PaeR7I CTCGAG 1 cut(s) 842
PagI TCATGA 1 cut(s) 898
PctI GAATGC 1 cut(s) 364
PdmI GAANNNNTTC 1 cut(s) 1130
PfeI GAWTC 3 cut(s) 162, 631, 688
Ple19I CGATCG 1 cut(s) 859
PleI GAGTC 3 cut(s) 32, 179, 1126
PpsI GAGTC 3 cut(s) 32, 179, 1126
Psp124BI GAGCTC 1 cut(s) 684
Psp6I CCWGG 2 cut(s) 197, 1068
PspEI GGTNACC 2 cut(s) 335, 485
PspGI CCWGG 2 cut(s) 197, 1068
PspN4I GGNNCC 3 cut(s) 157, 883, 1185
PspPI GGNCC 1 cut(s) 999
PstNI CAGNNNCTG 1 cut(s) 301
PsuI RGATCY 1 cut(s) 846
PvuI CGATCG 1 cut(s) 859
RsaI GTAC 2 cut(s) 558, 1185
RsaNI GTAC 2 cut(s) 557, 1184
SacI GAGCTC 1 cut(s) 684
SaqAI TTAA 5 cut(s) 23, 98, 917, 951, 1085
Sau3AI GATC 4 cut(s) 145, 846, 856, 895
Sau96I GGNCC 1 cut(s) 999
SchI GAGTC 3 cut(s) 32, 179, 1127
ScrFI CCNGG 3 cut(s) 199, 1015, 1070
SduI GDGCHC 3 cut(s) 225, 466, 684
SfaNI GCATC 5 cut(s) 203, 288, 775, 1157, 1227
Sfr274I CTCGAG 1 cut(s) 842
SlaI CTCGAG 1 cut(s) 842
SmlI CTYRAG 2 cut(s) 605, 842
SmoI CTYRAG 2 cut(s) 605, 842
SphI GCATGC 2 cut(s) 1024, 1028
Sse9I AATT 8 cut(s) 100, 491, 577, 707, 907, 936, 1053, 1082
SsiI CCGC 5 cut(s) 153, 744, 767, 985, 1153
SspMI CTAG 1 cut(s) 969
SstI GAGCTC 1 cut(s) 684
StyD4I CCNGG 3 cut(s) 197, 1013, 1068
TaaI ACNGT 5 cut(s) 341, 417, 663, 813, 1131
TaiI ACGT 1 cut(s) 542
TaqI TCGA 1 cut(s) 843
TaqII GACCGA 1 cut(s) 849
TasI AATT 8 cut(s) 100, 491, 577, 707, 907, 936, 1053, 1082
TauI GCSGC 3 cut(s) 156, 987, 1156
TfiI GAWTC 3 cut(s) 162, 631, 688
Tru1I TTAA 5 cut(s) 23, 98, 917, 951, 1085
Tru9I TTAA 5 cut(s) 23, 98, 917, 951, 1085
TscAI CASTG 2 cut(s) 444, 787
TseFI GTSAC 2 cut(s) 335, 485
TseI GCWGC 7 cut(s) 59, 275, 301, 741, 796, 806, 979
Tsp45I GTSAC 2 cut(s) 335, 485
TspDTI ATGAA 2 cut(s) 17, 102
TspGWI ACGGA 1 cut(s) 1120
TspRI CASTG 2 cut(s) 444, 787
VneI GTGCAC 1 cut(s) 462
XapI RAATTY 3 cut(s) 100, 707, 907
XceI RCATGY 3 cut(s) 1024, 1028, 1162
XhoI CTCGAG 1 cut(s) 842
XmiI GTMKAC 1 cut(s) 1138
XmnI GAANNNNTTC 1 cut(s) 1130
XspI CTAG 1 cut(s) 969
Zsp2I ATGCAT 2 cut(s) 1026, 1164
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.