FvH4_5g39720

WAT1-related protein At3g02690

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0009436
Physical Location & Seq
Reverse (-)
29301770 .. 29309003
7234 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g39720.t4

Sequence Viewer

Length: 1236 bp
ATGGCGTGGTTATCCTCAACCTCAGTTTCTGGTTGCGGCTGTCATTACAACCCTGGGACTGTTAGGCGGCCGCGCTGTTTGGTTAATGATATCAGGAAAAGAAACAGTAGTAGCTCATGGACAGCGCTATCATATTCTAATTGCCTTGCTCTTTTTCAATACAATCATATTCGCAAGCCCATTCATTTCGGTGCCAATTGCACAGCCAGTGGTGGTAATGAGACCACAGAGCTCAAAGATTCGTCTGACATGGTTGACTGCGTGGGAACAGGGCAAGACGTAGAGTGCGTGGTGACAGCCCAAGGAGAAGAAGATCGTGGACTGGAACTGGAATTAGTAGAACAATTGCAAGAATGGGCAGTGCTGGTCTCTCCCTTCTTCTTTTGGGGTACGGCTATGGTGGCCATGAAGGGCATTCTGCCAAAGACCGGCCCTTTCTTCATCTCAGCCTTCCGTCTGATTCCTGCTGGCTTCCTCCTCATCGCCTTCGCTGCCTACAGAGGCCGCCCCTTTCCTTCTGGCCTCACCGCTTGGCTTTCTATTGCGCTCTTCGGCCTCGTTGATGCTGCTTGTTTCCAAGGATTTCTTGCTCAAGGTTTGCAGAGGACGTCTGCTGGTCTGGGCAGCGTCATAATTGATTCACAACCTTTGACAGTCGCTGTACTTGCGGCCTTGTTCTTTGGTGAGTCCATTGGCTTCCTTGGAGCTGCAGGGCTTGTTCTTGGTGTCATTGGACTTTTACTTCTTGAGGCACCTTCACTTGCTTTTGATGGAAGTAACTTTTCATTGTGGCAAAATGGGGAGTGGTGGATGCTTCTCTCTGCTCAGAGCATGGCAGTTGGTACAGTCATGGTGCGTTGGGTTTCCAAGTACTCTGATCCTATTATGGCGACTGGATGGCATATGGTTATTGGCGGCCTGCCTCTTGTTATAATATCCATTCTTAATCATGACAATGCTGTCAGTGGGAGTCTCGTGGAGTTCACTGCAAATGATACATTGGCTCTCCTTTATACATCCATTTTTGGAAGTGCCATCAGCTATGGTATATACTTCTACAGTGCAACAAAAGGTAGCCTGACAAAACTCAGCTCCCTCACCTTTCTAACCCCAATGTTTGCTTCGATATTTGGGTTTCTATATCTTGGTGAGACCTTCTCACCCTTGCAACTGGGTGGAGCCATGATTACTATTGTTGCAATATACTTGGTTAACTACAGAACTATTGTTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

412

Amino Acids

44.25

Weight (kDa)

5.75

Isoelectric Point (pI)

34.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 123 - 249 1.9e-23 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 932
AasI GACNNNNNNGTC 1 cut(s) 959
AatII GACGTC 1 cut(s) 611
AccB1I GGYRCC 2 cut(s) 191, 751
AccII CGCG 1 cut(s) 73
AciI CCGC 7 cut(s) 36, 67, 71, 505, 528, 668, 915
AclWI GGATC 1 cut(s) 872
AcoI YGGCCR 2 cut(s) 68, 402
AcyI GRCGYC 1 cut(s) 608
AfaI GTAC 4 cut(s) 391, 663, 844, 872
AfeI AGCGCT 1 cut(s) 126
AfiI CCNNNNNNNGG 1 cut(s) 428
AgsI TTSAA 1 cut(s) 158
AjnI CCWGG 1 cut(s) 52
AjuI GAANNNNNNNTTGG 2 cut(s) 1105, 1137
AluBI AGCT 5 cut(s) 114, 232, 707, 1041, 1092
AluI AGCT 5 cut(s) 114, 232, 707, 1041, 1092
Alw21I GWGCWC 1 cut(s) 234
Alw26I GTCTC 4 cut(s) 215, 373, 977, 1145
AlwI GGATC 1 cut(s) 872
AlwNI CAGNNNCTG 2 cut(s) 29, 659
Aor51HI AGCGCT 1 cut(s) 126
AoxI GGCC 8 cut(s) 68, 402, 430, 502, 520, 553, 669, 916
ApeKI GCWGC 4 cut(s) 491, 566, 624, 707
AspLEI GCGC 3 cut(s) 75, 127, 547
AspS9I GGNCC 1 cut(s) 431
AsuHPI GGTGA 6 cut(s) 304, 517, 695, 1090, 1152, 1160
BalI TGGCCA 1 cut(s) 404
BanI GGYRCC 2 cut(s) 191, 751
BanII GRGCYC 1 cut(s) 234
BauI CACGAG 1 cut(s) 974
Bbv12I GWGCWC 1 cut(s) 234
BbvI GCAGC 4 cut(s) 478, 553, 636, 694
BccI CCATC 3 cut(s) 764, 891, 1043
BceAI ACGGC 1 cut(s) 408
BciT130I CCWGG 1 cut(s) 54
BcoDI GTCTC 4 cut(s) 215, 373, 977, 1145
BfmI CTRYAG 4 cut(s) 496, 708, 1057, 1216
BfoI RGCGCY 1 cut(s) 128
BmcAI AGTACT 1 cut(s) 872
Bme1390I CCNGG 1 cut(s) 54
BmgT120I GGNCC 1 cut(s) 431
BmiI GGNNCC 3 cut(s) 193, 753, 1180
BmrFI CCNGG 1 cut(s) 54
BmrI ACTGGG 1 cut(s) 1181
BmsI GCATC 2 cut(s) 553, 801
BmuI ACTGGG 1 cut(s) 1181
BplI GAGNNNNNCTC 2 cut(s) 1142, 1174
BpuEI CTTGAG 2 cut(s) 576, 767
BsaHI GRCGYC 1 cut(s) 608
BsaI GGTCTC 3 cut(s) 215, 373, 1145
BsaJI CCNNGG 5 cut(s) 52, 53, 301, 577, 700
Bsc4I CCNNNNNNNGG 1 cut(s) 428
Bse118I RCCGGY 1 cut(s) 428
Bse1I ACTGG 5 cut(s) 207, 327, 333, 898, 1176
BseBI CCWGG 1 cut(s) 54
BseDI CCNNGG 5 cut(s) 52, 53, 301, 577, 700
BseGI GGATG 3 cut(s) 816, 902, 1016
BseLI CCNNNNNNNGG 1 cut(s) 428
BseMII CTCAG 4 cut(s) 36, 459, 839, 1102
BseNI ACTGG 5 cut(s) 207, 327, 333, 898, 1176
BseRI GAGGAG 1 cut(s) 467
BseX3I CGGCCG 1 cut(s) 68
BseXI GCAGC 4 cut(s) 478, 553, 636, 694
Bsh1236I CGCG 1 cut(s) 73
Bsh1285I CGRYCG 1 cut(s) 71
BshFI GGCC 8 cut(s) 70, 404, 432, 504, 522, 555, 671, 918
BshNI GGYRCC 2 cut(s) 191, 751
BsiEI CGRYCG 1 cut(s) 71
BsiHKAI GWGCWC 1 cut(s) 234
BsiSI CCGG 1 cut(s) 429
BslFI GGGAC 1 cut(s) 70
BslI CCNNNNNNNGG 1 cut(s) 428
BsmAI GTCTC 4 cut(s) 215, 373, 977, 1145
BsmFI GGGAC 1 cut(s) 70
BsmI GAATGC 1 cut(s) 414
BsnI GGCC 8 cut(s) 70, 404, 432, 504, 522, 555, 671, 918
Bso31I GGTCTC 3 cut(s) 215, 373, 1145
Bsp1286I GDGCHC 1 cut(s) 234
Bsp143I GATC 2 cut(s) 313, 877
BspACI CCGC 7 cut(s) 36, 67, 71, 505, 528, 668, 915
BspANI GGCC 8 cut(s) 70, 404, 432, 504, 522, 555, 671, 918
BspCNI CTCAG 4 cut(s) 35, 458, 838, 1101
BspFNI CGCG 1 cut(s) 73
BspHI TCATGA 1 cut(s) 949
BspLI GGNNCC 3 cut(s) 193, 753, 1180
BspMAI CTGCAG 1 cut(s) 712
BspPI GGATC 1 cut(s) 872
BspQI GCTCTTC 1 cut(s) 554
BspT107I GGYRCC 2 cut(s) 191, 751
BspTNI GGTCTC 3 cut(s) 215, 373, 1145
BsrFI RCCGGY 1 cut(s) 428
BsrI ACTGG 5 cut(s) 207, 327, 333, 898, 1176
BssAI RCCGGY 1 cut(s) 428
BssECI CCNNGG 5 cut(s) 52, 53, 301, 577, 700
BssMI GATC 2 cut(s) 313, 877
BssNI GRCGYC 1 cut(s) 608
BssSI CACGAG 1 cut(s) 974
BssT1I CCWWGG 3 cut(s) 301, 577, 700
Bst2BI CACGAG 1 cut(s) 974
Bst2UI CCWGG 1 cut(s) 54
Bst4CI ACNGT 5 cut(s) 61, 107, 655, 847, 1061
Bst6I CTCTTC 1 cut(s) 554
BstACI GRCGYC 1 cut(s) 608
BstC8I GCNNGC 3 cut(s) 176, 469, 920
BstDEI CTNAG 4 cut(s) 22, 445, 825, 1088
BstF5I GGATG 3 cut(s) 816, 902, 1016
BstFNI CGCG 1 cut(s) 73
BstH2I RGCGCY 1 cut(s) 128
BstHHI GCGC 3 cut(s) 75, 127, 547
BstKTI GATC 2 cut(s) 316, 880
BstMAI GTCTC 4 cut(s) 215, 373, 977, 1145
BstMBI GATC 2 cut(s) 313, 877
BstMCI CGRYCG 1 cut(s) 71
BstMWI GCNNNNNNNGC 3 cut(s) 401, 491, 665
BstNI CCWGG 1 cut(s) 54
BstSCI CCNGG 1 cut(s) 52
BstSFI CTRYAG 4 cut(s) 496, 708, 1057, 1216
BstUI CGCG 1 cut(s) 73
BstV1I GCAGC 4 cut(s) 478, 553, 636, 694
BstZI CGGCCG 1 cut(s) 68
BsuRI GGCC 8 cut(s) 70, 404, 432, 504, 522, 555, 671, 918
BtgZI GCGATG 1 cut(s) 466
BtsCI GGATG 3 cut(s) 816, 902, 1016
BtsI GCAGTG 2 cut(s) 366, 984
BtsIMutI CAGTG 5 cut(s) 214, 366, 970, 984, 1066
Cac8I GCNNGC 3 cut(s) 176, 469, 920
CaiI CAGNNNCTG 2 cut(s) 29, 659
CciI TCATGA 1 cut(s) 949
CciNI GCGGCCGC 1 cut(s) 68
CfoI GCGC 3 cut(s) 75, 127, 547
Cfr10I RCCGGY 1 cut(s) 428
Cfr13I GGNCC 1 cut(s) 431
CseI GACGC 1 cut(s) 616
Csp6I GTAC 4 cut(s) 390, 662, 843, 871
CviAII CATG 7 cut(s) 117, 250, 406, 832, 850, 950, 1183
CviQI GTAC 4 cut(s) 390, 662, 843, 871
DdeI CTNAG 4 cut(s) 22, 445, 825, 1088
DpnI GATC 2 cut(s) 315, 879
DpnII GATC 2 cut(s) 313, 877
DrdI GACNNNNNNGTC 1 cut(s) 959
DseDI GACNNNNNNGTC 1 cut(s) 959
EaeI YGGCCR 2 cut(s) 68, 402
EagI CGGCCG 1 cut(s) 68
Eam1104I CTCTTC 1 cut(s) 554
EarI CTCTTC 1 cut(s) 554
Ecl136II GAGCTC 1 cut(s) 232
EclXI CGGCCG 1 cut(s) 68
Eco130I CCWWGG 3 cut(s) 301, 577, 700
Eco24I GRGCYC 1 cut(s) 234
Eco31I GGTCTC 3 cut(s) 215, 373, 1145
Eco32I GATATC 1 cut(s) 91
Eco47III AGCGCT 1 cut(s) 126
Eco52I CGGCCG 1 cut(s) 68
Eco53kI GAGCTC 1 cut(s) 232
EcoICRI GAGCTC 1 cut(s) 232
EcoRII CCWGG 1 cut(s) 52
EcoRV GATATC 1 cut(s) 91
EcoT14I CCWWGG 3 cut(s) 301, 577, 700
EcoT38I GRGCYC 1 cut(s) 234
ErhI CCWWGG 3 cut(s) 301, 577, 700
FaeI CATG 7 cut(s) 120, 253, 409, 835, 853, 953, 1186
FalI AAGNNNNNCTT 2 cut(s) 570, 602
FaqI GGGAC 1 cut(s) 70
FatI CATG 7 cut(s) 116, 249, 405, 831, 849, 949, 1182
FauNDI CATATG 1 cut(s) 903
FokI GGATG 3 cut(s) 823, 909, 1003
FriOI GRGCYC 1 cut(s) 234
GlaI GCGC 3 cut(s) 74, 126, 546
HaeII RGCGCY 1 cut(s) 128
HaeIII GGCC 8 cut(s) 70, 404, 432, 504, 522, 555, 671, 918
HapII CCGG 1 cut(s) 429
HgaI GACGC 1 cut(s) 616
HhaI GCGC 3 cut(s) 75, 127, 547
Hin1I GRCGYC 1 cut(s) 608
Hin1II CATG 7 cut(s) 120, 253, 409, 835, 853, 953, 1186
Hin6I GCGC 3 cut(s) 73, 125, 545
HinP1I GCGC 3 cut(s) 73, 125, 545
HincII GTYRAC 2 cut(s) 256, 1213
HindII GTYRAC 2 cut(s) 256, 1213
HinfI GANTC 5 cut(s) 239, 460, 638, 686, 970
HpaI GTTAAC 1 cut(s) 1213
HpaII CCGG 1 cut(s) 429
HphI GGTGA 6 cut(s) 304, 517, 695, 1090, 1152, 1160
Hpy166II GTNNAC 4 cut(s) 256, 320, 984, 1213
Hpy188I TCNGA 4 cut(s) 247, 459, 828, 877
Hpy188III TCNNGA 3 cut(s) 94, 746, 950
Hpy8I GTNNAC 4 cut(s) 256, 320, 984, 1213
HpyAV CCTTC 7 cut(s) 385, 403, 460, 496, 525, 765, 1165
HpyCH4III ACNGT 5 cut(s) 61, 107, 655, 847, 1061
HpyCH4IV ACGT 2 cut(s) 279, 608
HpyCH4V TGCA 8 cut(s) 201, 349, 601, 710, 989, 1064, 1168, 1199
HpyF10VI GCNNNNNNNGC 3 cut(s) 401, 491, 665
HpyF3I CTNAG 4 cut(s) 22, 445, 825, 1088
HpySE526I ACGT 2 cut(s) 279, 608
Hsp92I GRCGYC 1 cut(s) 608
Hsp92II CATG 7 cut(s) 120, 253, 409, 835, 853, 953, 1186
HspAI GCGC 3 cut(s) 73, 125, 545
KspAI GTTAAC 1 cut(s) 1213
Kzo9I GATC 2 cut(s) 313, 877
LguI GCTCTTC 1 cut(s) 554
LmnI GCTCC 3 cut(s) 704, 1097, 1178
Lsp1109I GCAGC 4 cut(s) 478, 553, 636, 694
LweI GCATC 2 cut(s) 553, 801
MaeII ACGT 2 cut(s) 279, 608
MaeIII GTNAC 2 cut(s) 292, 776
MalI GATC 2 cut(s) 315, 879
MboI GATC 2 cut(s) 313, 877
MboII GAAGA 5 cut(s) 320, 323, 370, 430, 541
MfeI CAATTG 2 cut(s) 196, 344
MhlI GDGCHC 1 cut(s) 234
MlsI TGGCCA 1 cut(s) 404
MluCI AATT 5 cut(s) 139, 196, 332, 344, 633
MluNI TGGCCA 1 cut(s) 404
MlyI GAGTC 2 cut(s) 695, 979
Mox20I TGGCCA 1 cut(s) 404
MscI TGGCCA 1 cut(s) 404
MseI TTAA 4 cut(s) 84, 945, 1212, 1230
MslI CAYNNNNRTG 2 cut(s) 189, 954
Msp20I TGGCCA 1 cut(s) 404
MspI CCGG 1 cut(s) 429
MspR9I CCNGG 1 cut(s) 54
MunI CAATTG 2 cut(s) 196, 344
Mva1269I GAATGC 1 cut(s) 414
MvaI CCWGG 1 cut(s) 54
MvnI CGCG 1 cut(s) 73
MwoI GCNNNNNNNGC 3 cut(s) 401, 491, 665
NdeI CATATG 1 cut(s) 903
NdeII GATC 2 cut(s) 313, 877
NlaIII CATG 7 cut(s) 120, 253, 409, 835, 853, 953, 1186
NlaIV GGNNCC 3 cut(s) 193, 753, 1180
NmuCI GTSAC 1 cut(s) 292
NotI GCGGCCGC 1 cut(s) 68
PagI TCATGA 1 cut(s) 949
PasI CCCWGGG 1 cut(s) 53
PciSI GCTCTTC 1 cut(s) 554
PcsI WCGNNNNNNNCGW 1 cut(s) 285
PctI GAATGC 1 cut(s) 414
PfeI GAWTC 3 cut(s) 239, 460, 638
PleI GAGTC 2 cut(s) 694, 978
PpsI GAGTC 2 cut(s) 694, 978
PsiI TTATAA 1 cut(s) 932
Psp124BI GAGCTC 1 cut(s) 234
Psp6I CCWGG 1 cut(s) 52
PspGI CCWGG 1 cut(s) 52
PspN4I GGNNCC 3 cut(s) 193, 753, 1180
PspPI GGNCC 1 cut(s) 431
PstI CTGCAG 1 cut(s) 712
PstNI CAGNNNCTG 2 cut(s) 29, 659
RsaI GTAC 4 cut(s) 391, 663, 844, 872
RsaNI GTAC 4 cut(s) 390, 662, 843, 871
RseI CAYNNNNRTG 2 cut(s) 189, 954
SacI GAGCTC 1 cut(s) 234
SapI GCTCTTC 1 cut(s) 554
SaqAI TTAA 4 cut(s) 84, 945, 1212, 1230
Sau3AI GATC 2 cut(s) 313, 877
Sau96I GGNCC 1 cut(s) 431
ScaI AGTACT 1 cut(s) 872
SchI GAGTC 2 cut(s) 695, 979
ScrFI CCNGG 1 cut(s) 54
SduI GDGCHC 1 cut(s) 234
SfaNI GCATC 2 cut(s) 553, 801
SfcI CTRYAG 4 cut(s) 496, 708, 1057, 1216
SmiMI CAYNNNNRTG 2 cut(s) 189, 954
SmlI CTYRAG 2 cut(s) 591, 746
SmoI CTYRAG 2 cut(s) 591, 746
Sse9I AATT 5 cut(s) 139, 196, 332, 344, 633
SsiI CCGC 7 cut(s) 36, 67, 71, 505, 528, 668, 915
SstI GAGCTC 1 cut(s) 234
StyD4I CCNGG 1 cut(s) 52
StyI CCWWGG 3 cut(s) 301, 577, 700
TaaI ACNGT 5 cut(s) 61, 107, 655, 847, 1061
TaiI ACGT 2 cut(s) 282, 611
TaqI TCGA 1 cut(s) 1124
TasI AATT 5 cut(s) 139, 196, 332, 344, 633
TatI WGTACW 2 cut(s) 661, 870
TauI GCSGC 6 cut(s) 39, 70, 73, 507, 671, 918
TfiI GAWTC 3 cut(s) 239, 460, 638
Tru1I TTAA 4 cut(s) 84, 945, 1212, 1230
Tru9I TTAA 4 cut(s) 84, 945, 1212, 1230
TscAI CASTG 5 cut(s) 214, 366, 970, 991, 1066
TseFI GTSAC 1 cut(s) 292
TseI GCWGC 4 cut(s) 491, 566, 624, 707
Tsp45I GTSAC 1 cut(s) 292
TspDTI ATGAA 4 cut(s) 173, 422, 430, 774
TspGWI ACGGA 1 cut(s) 443
TspRI CASTG 5 cut(s) 214, 366, 970, 991, 1066
ZraI GACGTC 1 cut(s) 609
ZrmI AGTACT 1 cut(s) 872
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.