Rh7DG490200

WAT1-related protein At3g02690

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0009436
Physical Location & Seq
Reverse (-)
69550858 .. 69556479
5622 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG490200.1

Sequence Viewer

Length: 1077 bp
ATGGGGTTGTTATCCTCAGTTTCTGCTTGCTACCGTTGTGGCTGTTATTACGACCCTGGGACTGTCCGGCGGCCGAATTTAGATCGACGTTTGGTTAATGATATCAGGAAAAGAAACAGTTGGCCAAGGAAAGCGCTATCATATTCTAATTGCCTCCTTATTTCTCAATATAGTCATAATCGCAAACCACCAACGCACCCGAATCCACACAATAACTACTACAAGTCCATTCATTTTGGTGCCAATTGCACTGGCAGTAGCGGTAGTGAGACCACCGAGCTCGAAGATTCGTCTGACATCGTTGATTGTGTGGGAACAGGGCAAGACGACGTAGTAGAGTGCGTGGTCACAGCCGAAGAAACTGGTGGTCCTGGATTGGAACTGGAATTACTAGAACAAGTGCGAGAATGGGCAGTACTGGTCTCTCCCTTCTTCTTTTGGGGTACCGCTATGGTGGCCATGAAGGGGATTCTGCCAAAGACGGGCCCTTTCTTCATCTCAGCCTTCCGTCTAATTCCTGCTGGCTTTCTCCTCGTTGCCTTCGCCGCCTACAGAGGCCGCCCCTTTCCCTCTGGCCTCACCGCTTGGCTTTCTATTGCGCTTTTCGGCCTCGTTGATGCTGCTTGTTTTCAAGGATTTCTTGCTCAAGGTTTGCAGAGGACATCTGCTGGTCTTGGCAGCGTTATAATTGATTCACAACCTTTGACAGTCGCTATACTTGCGGCCTTGTTATTTGGTGAGTCCATTGGCTTCCTTGGAGCTGCAGGGCTTGTTCTTGGCGTCATTGGACTTTTACTTCTTGAGGCACCTTCACTTGCTTTTGATGGAAGTAACTCTTCATTGTGGCAAAGTGGGGAGTGGTGGATGCTTCTCTCAGCTCAGAGCATGGCAGTTGGTACAGTCATGGTGCGTTGGGTTTCTAAATACTCTGATCCTATTATGGCAACTGGATGGGTAGGCTATTCAACTCACTTGATAATAACTTATAATCGATACTGTATCCTGGGTTCATCTTGTAATTTTTTACTGCAATTTCATAAGTTGGGCTACTATTTTAAGCATTCCTACCTTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

358

Amino Acids

39.12

Weight (kDa)

7.02

Isoelectric Point (pI)

39.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 141 - 267 3.2e-23 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 686, 987
Acc65I GGTACC 1 cut(s) 443
AccB1I GGYRCC 3 cut(s) 239, 443, 805
AciI CCGC 7 cut(s) 70, 261, 447, 546, 559, 582, 722
AclWI GGATC 1 cut(s) 926
AcoI YGGCCR 3 cut(s) 71, 122, 456
AcsI RAATTY 1 cut(s) 76
AcyI GRCGYC 1 cut(s) 780
AfaI GTAC 3 cut(s) 417, 445, 898
AfeI AGCGCT 1 cut(s) 135
AfiI CCNNNNNNNGG 2 cut(s) 465, 482
AgsI TTSAA 2 cut(s) 632, 966
AjnI CCWGG 3 cut(s) 55, 370, 1002
AluBI AGCT 3 cut(s) 280, 761, 878
AluI AGCT 3 cut(s) 280, 761, 878
Alw21I GWGCWC 1 cut(s) 282
Alw26I GTCTC 2 cut(s) 263, 427
AlwI GGATC 1 cut(s) 926
AlwNI CAGNNNCTG 1 cut(s) 23
Aor51HI AGCGCT 1 cut(s) 135
AoxI GGCC 8 cut(s) 71, 122, 456, 484, 556, 574, 607, 723
ApaI GGGCCC 1 cut(s) 488
ApeKI GCWGC 3 cut(s) 620, 678, 761
ApoI RAATTY 1 cut(s) 76
Asp718I GGTACC 1 cut(s) 443
AspLEI GCGC 2 cut(s) 136, 601
AspS9I GGNCC 3 cut(s) 368, 484, 485
AsuHPI GGTGA 2 cut(s) 571, 749
AvaII GGWCC 1 cut(s) 368
BaeGI GKGCMC 1 cut(s) 488
BalI TGGCCA 2 cut(s) 124, 458
BanI GGYRCC 3 cut(s) 239, 443, 805
BanII GRGCYC 2 cut(s) 282, 488
Bbv12I GWGCWC 1 cut(s) 282
BbvI GCAGC 3 cut(s) 607, 690, 748
BccI CCATC 2 cut(s) 818, 945
BciT130I CCWGG 3 cut(s) 57, 372, 1004
BciVI GTATCC 1 cut(s) 1010
BcoDI GTCTC 2 cut(s) 263, 427
BfaI CTAG 1 cut(s) 392
BfmI CTRYAG 2 cut(s) 550, 762
BfoI RGCGCY 1 cut(s) 137
BfuI GTATCC 1 cut(s) 1010
BisI GCNGC 7 cut(s) 71, 546, 559, 621, 679, 723, 762
BlsI GCNGC 7 cut(s) 72, 547, 560, 622, 680, 724, 763
BmcAI AGTACT 1 cut(s) 417
Bme1390I CCNGG 3 cut(s) 57, 372, 1004
Bme18I GGWCC 1 cut(s) 368
BmgT120I GGNCC 3 cut(s) 368, 484, 485
BmiI GGNNCC 4 cut(s) 241, 445, 486, 807
BmrFI CCNGG 3 cut(s) 57, 372, 1004
BmsI GCATC 2 cut(s) 607, 855
BpuEI CTTGAG 2 cut(s) 630, 821
Bsa29I ATCGAT 1 cut(s) 991
BsaHI GRCGYC 1 cut(s) 780
BsaI GGTCTC 2 cut(s) 263, 427
BsaJI CCNNGG 5 cut(s) 55, 56, 125, 754, 1003
Bsc4I CCNNNNNNNGG 2 cut(s) 465, 482
Bse1I ACTGG 5 cut(s) 256, 367, 387, 423, 952
BseBI CCWGG 3 cut(s) 57, 372, 1004
BseCI ATCGAT 1 cut(s) 991
BseDI CCNNGG 5 cut(s) 55, 56, 125, 754, 1003
BseGI GGATG 2 cut(s) 870, 956
BseLI CCNNNNNNNGG 2 cut(s) 465, 482
BseMII CTCAG 4 cut(s) 30, 513, 888, 893
BseNI ACTGG 5 cut(s) 256, 367, 387, 423, 952
BseRI GAGGAG 1 cut(s) 521
BseSI GKGCMC 1 cut(s) 488
BseX3I CGGCCG 1 cut(s) 71
BseXI GCAGC 3 cut(s) 607, 690, 748
Bsh1285I CGRYCG 1 cut(s) 74
BshFI GGCC 8 cut(s) 73, 124, 458, 486, 558, 576, 609, 725
BshNI GGYRCC 3 cut(s) 239, 443, 805
BshVI ATCGAT 1 cut(s) 991
BsiEI CGRYCG 1 cut(s) 74
BsiHKAI GWGCWC 1 cut(s) 282
BsiSI CCGG 1 cut(s) 67
BslFI GGGAC 1 cut(s) 73
BslI CCNNNNNNNGG 2 cut(s) 465, 482
BsmAI GTCTC 2 cut(s) 263, 427
BsmFI GGGAC 1 cut(s) 73
BsmI GAATGC 1 cut(s) 1060
BsnI GGCC 8 cut(s) 73, 124, 458, 486, 558, 576, 609, 725
Bso31I GGTCTC 2 cut(s) 263, 427
Bsp120I GGGCCC 1 cut(s) 484
Bsp1286I GDGCHC 2 cut(s) 282, 488
Bsp143I GATC 2 cut(s) 82, 931
BspACI CCGC 7 cut(s) 70, 261, 447, 546, 559, 582, 722
BspANI GGCC 8 cut(s) 73, 124, 458, 486, 558, 576, 609, 725
BspCNI CTCAG 4 cut(s) 29, 512, 887, 892
BspDI ATCGAT 1 cut(s) 991
BspLI GGNNCC 4 cut(s) 241, 445, 486, 807
BspMAI CTGCAG 1 cut(s) 766
BspPI GGATC 1 cut(s) 926
BspT107I GGYRCC 3 cut(s) 239, 443, 805
BspTNI GGTCTC 2 cut(s) 263, 427
BsrI ACTGG 5 cut(s) 256, 367, 387, 423, 952
BssECI CCNNGG 5 cut(s) 55, 56, 125, 754, 1003
BssMI GATC 2 cut(s) 82, 931
BssNI GRCGYC 1 cut(s) 780
BssT1I CCWWGG 2 cut(s) 125, 754
Bst2UI CCWGG 3 cut(s) 57, 372, 1004
Bst4CI ACNGT 6 cut(s) 35, 64, 119, 709, 901, 998
Bst6I CTCTTC 1 cut(s) 841
BstACI GRCGYC 1 cut(s) 780
BstC8I GCNNGC 2 cut(s) 28, 523
BstDEI CTNAG 4 cut(s) 16, 499, 874, 879
BstF5I GGATG 2 cut(s) 870, 956
BstH2I RGCGCY 1 cut(s) 137
BstHHI GCGC 2 cut(s) 136, 601
BstKTI GATC 2 cut(s) 85, 934
BstMAI GTCTC 2 cut(s) 263, 427
BstMBI GATC 2 cut(s) 82, 931
BstMCI CGRYCG 1 cut(s) 74
BstMWI GCNNNNNNNGC 3 cut(s) 455, 545, 719
BstNI CCWGG 3 cut(s) 57, 372, 1004
BstSCI CCNGG 3 cut(s) 55, 370, 1002
BstSFI CTRYAG 2 cut(s) 550, 762
BstSLI GKGCMC 1 cut(s) 488
BstV1I GCAGC 3 cut(s) 607, 690, 748
BstZI CGGCCG 1 cut(s) 71
Bsu15I ATCGAT 1 cut(s) 991
BsuI GTATCC 1 cut(s) 1010
BsuRI GGCC 8 cut(s) 73, 124, 458, 486, 558, 576, 609, 725
BsuTUI ATCGAT 1 cut(s) 991
BtsCI GGATG 2 cut(s) 870, 956
BtsIMutI CAGTG 1 cut(s) 249
Cac8I GCNNGC 2 cut(s) 28, 523
CaiI CAGNNNCTG 1 cut(s) 23
CfoI GCGC 2 cut(s) 136, 601
Cfr13I GGNCC 3 cut(s) 368, 484, 485
ClaI ATCGAT 1 cut(s) 991
CseI GACGC 1 cut(s) 769
Csp6I GTAC 3 cut(s) 416, 444, 897
CviAII CATG 3 cut(s) 460, 886, 904
CviQI GTAC 3 cut(s) 416, 444, 897
DdeI CTNAG 4 cut(s) 16, 499, 874, 879
DpnI GATC 2 cut(s) 84, 933
DpnII GATC 2 cut(s) 82, 931
EaeI YGGCCR 3 cut(s) 71, 122, 456
EagI CGGCCG 1 cut(s) 71
Eam1104I CTCTTC 1 cut(s) 841
EarI CTCTTC 1 cut(s) 841
Ecl136II GAGCTC 1 cut(s) 280
EclXI CGGCCG 1 cut(s) 71
Eco130I CCWWGG 2 cut(s) 125, 754
Eco24I GRGCYC 2 cut(s) 282, 488
Eco31I GGTCTC 2 cut(s) 263, 427
Eco32I GATATC 1 cut(s) 103
Eco47I GGWCC 1 cut(s) 368
Eco47III AGCGCT 1 cut(s) 135
Eco52I CGGCCG 1 cut(s) 71
Eco53kI GAGCTC 1 cut(s) 280
EcoICRI GAGCTC 1 cut(s) 280
EcoO109I RGGNCCY 1 cut(s) 485
EcoRII CCWGG 3 cut(s) 55, 370, 1002
EcoRV GATATC 1 cut(s) 103
EcoT14I CCWWGG 2 cut(s) 125, 754
EcoT38I GRGCYC 2 cut(s) 282, 488
ErhI CCWWGG 2 cut(s) 125, 754
FaeI CATG 3 cut(s) 463, 889, 907
FalI AAGNNNNNCTT 4 cut(s) 624, 656, 820, 852
FaqI GGGAC 1 cut(s) 73
FatI CATG 3 cut(s) 459, 885, 903
Fnu4HI GCNGC 7 cut(s) 71, 546, 559, 621, 679, 723, 762
FokI GGATG 2 cut(s) 877, 963
FriOI GRGCYC 2 cut(s) 282, 488
Fsp4HI GCNGC 7 cut(s) 71, 546, 559, 621, 679, 723, 762
FspBI CTAG 1 cut(s) 392
GlaI GCGC 2 cut(s) 135, 600
GluI GCNGC 7 cut(s) 71, 546, 559, 621, 679, 723, 762
HaeII RGCGCY 1 cut(s) 137
HaeIII GGCC 8 cut(s) 73, 124, 458, 486, 558, 576, 609, 725
HapII CCGG 1 cut(s) 67
HgaI GACGC 1 cut(s) 769
HhaI GCGC 2 cut(s) 136, 601
Hin1I GRCGYC 1 cut(s) 780
Hin1II CATG 3 cut(s) 463, 889, 907
Hin6I GCGC 2 cut(s) 134, 599
HinP1I GCGC 2 cut(s) 134, 599
HinfI GANTC 5 cut(s) 202, 287, 469, 692, 740
HpaII CCGG 1 cut(s) 67
HphI GGTGA 2 cut(s) 571, 749
Hpy188I TCNGA 3 cut(s) 295, 882, 931
Hpy188III TCNNGA 2 cut(s) 106, 800
Hpy99I CGWCG 2 cut(s) 90, 332
HpyAV CCTTC 5 cut(s) 439, 457, 514, 550, 819
HpyCH4III ACNGT 6 cut(s) 35, 64, 119, 709, 901, 998
HpyCH4IV ACGT 2 cut(s) 88, 330
HpyCH4V TGCA 4 cut(s) 249, 655, 764, 1030
HpyF10VI GCNNNNNNNGC 3 cut(s) 455, 545, 719
HpyF3I CTNAG 4 cut(s) 16, 499, 874, 879
HpySE526I ACGT 2 cut(s) 88, 330
Hsp92I GRCGYC 1 cut(s) 780
Hsp92II CATG 3 cut(s) 463, 889, 907
HspAI GCGC 2 cut(s) 134, 599
KpnI GGTACC 1 cut(s) 447
Kzo9I GATC 2 cut(s) 82, 931
LmnI GCTCC 1 cut(s) 758
Lsp1109I GCAGC 3 cut(s) 607, 690, 748
LweI GCATC 2 cut(s) 607, 855
MaeI CTAG 1 cut(s) 392
MaeII ACGT 2 cut(s) 88, 330
MaeIII GTNAC 2 cut(s) 346, 830
MalI GATC 2 cut(s) 84, 933
MboI GATC 2 cut(s) 82, 931
MboII GAAGA 5 cut(s) 296, 368, 424, 484, 828
MfeI CAATTG 1 cut(s) 244
MhlI GDGCHC 2 cut(s) 282, 488
MlsI TGGCCA 2 cut(s) 124, 458
MluCI AATT 8 cut(s) 76, 148, 244, 386, 513, 687, 1018, 1031
MluNI TGGCCA 2 cut(s) 124, 458
MlyI GAGTC 1 cut(s) 749
MnlI CCTC 9 cut(s) 25, 164, 542, 548, 580, 587, 620, 651, 796
Mox20I TGGCCA 2 cut(s) 124, 458
MscI TGGCCA 2 cut(s) 124, 458
MseI TTAA 2 cut(s) 96, 1056
MslI CAYNNNNRTG 1 cut(s) 237
Msp20I TGGCCA 2 cut(s) 124, 458
MspI CCGG 1 cut(s) 67
MspR9I CCNGG 3 cut(s) 57, 372, 1004
MunI CAATTG 1 cut(s) 244
Mva1269I GAATGC 1 cut(s) 1060
MvaI CCWGG 3 cut(s) 57, 372, 1004
MwoI GCNNNNNNNGC 3 cut(s) 455, 545, 719
NdeII GATC 2 cut(s) 82, 931
NlaIII CATG 3 cut(s) 463, 889, 907
NlaIV GGNNCC 4 cut(s) 241, 445, 486, 807
NmuCI GTSAC 1 cut(s) 346
PasI CCCWGGG 1 cut(s) 56
PctI GAATGC 1 cut(s) 1060
PfeI GAWTC 4 cut(s) 202, 287, 469, 692
PfoI TCCNGGA 1 cut(s) 370
PkrI GCNGC 7 cut(s) 72, 547, 560, 622, 680, 724, 763
PleI GAGTC 1 cut(s) 748
PpsI GAGTC 1 cut(s) 748
PsiI TTATAA 2 cut(s) 686, 987
Psp124BI GAGCTC 1 cut(s) 282
Psp6I CCWGG 3 cut(s) 55, 370, 1002
PspGI CCWGG 3 cut(s) 55, 370, 1002
PspN4I GGNNCC 4 cut(s) 241, 445, 486, 807
PspOMI GGGCCC 1 cut(s) 484
PspPI GGNCC 3 cut(s) 368, 484, 485
PsrI GAACNNNNNNTAC 4 cut(s) 372, 404, 991, 1023
PstI CTGCAG 1 cut(s) 766
PstNI CAGNNNCTG 1 cut(s) 23
RsaI GTAC 3 cut(s) 417, 445, 898
RsaNI GTAC 3 cut(s) 416, 444, 897
RseI CAYNNNNRTG 1 cut(s) 237
SacI GAGCTC 1 cut(s) 282
SaqAI TTAA 2 cut(s) 96, 1056
SatI GCNGC 7 cut(s) 71, 546, 559, 621, 679, 723, 762
Sau3AI GATC 2 cut(s) 82, 931
Sau96I GGNCC 3 cut(s) 368, 484, 485
ScaI AGTACT 1 cut(s) 417
SchI GAGTC 1 cut(s) 749
ScrFI CCNGG 3 cut(s) 57, 372, 1004
SduI GDGCHC 2 cut(s) 282, 488
SetI ASST 9 cut(s) 91, 282, 333, 652, 703, 763, 811, 880, 1071
SfaNI GCATC 2 cut(s) 607, 855
SfcI CTRYAG 2 cut(s) 550, 762
SinI GGWCC 1 cut(s) 368
SmiMI CAYNNNNRTG 1 cut(s) 237
SmlI CTYRAG 2 cut(s) 645, 800
SmoI CTYRAG 2 cut(s) 645, 800
Sse9I AATT 8 cut(s) 76, 148, 244, 386, 513, 687, 1018, 1031
SsiI CCGC 7 cut(s) 70, 261, 447, 546, 559, 582, 722
SspMI CTAG 1 cut(s) 392
SstI GAGCTC 1 cut(s) 282
StyD4I CCNGG 3 cut(s) 55, 370, 1002
StyI CCWWGG 2 cut(s) 125, 754
TaaI ACNGT 6 cut(s) 35, 64, 119, 709, 901, 998
TaiI ACGT 2 cut(s) 91, 333
TaqI TCGA 3 cut(s) 85, 282, 991
TasI AATT 8 cut(s) 76, 148, 244, 386, 513, 687, 1018, 1031
TatI WGTACW 1 cut(s) 415
TauI GCSGC 4 cut(s) 73, 548, 561, 725
TfiI GAWTC 4 cut(s) 202, 287, 469, 692
Tru1I TTAA 2 cut(s) 96, 1056
Tru9I TTAA 2 cut(s) 96, 1056
TscAI CASTG 1 cut(s) 256
TseFI GTSAC 1 cut(s) 346
TseI GCWGC 3 cut(s) 620, 678, 761
Tsp45I GTSAC 1 cut(s) 346
TspDTI ATGAA 6 cut(s) 221, 476, 484, 828, 999, 1025
TspGWI ACGGA 1 cut(s) 497
TspRI CASTG 1 cut(s) 256
VpaK11BI GGWCC 1 cut(s) 368
XapI RAATTY 1 cut(s) 76
XspI CTAG 1 cut(s) 392
ZrmI AGTACT 1 cut(s) 417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.