Rh7BG476400

WAT1-related protein At3g02690

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0009436
Physical Location & Seq
Reverse (-)
56089407 .. 56098043
8637 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG476400.1

Sequence Viewer

Length: 981 bp
ATGGGGTTGTTATCCTCAGTTTCTGCTTGCTACCGTTGTGGCTGTTATTACGACCCTGGGACTGTTGGGCGGCCGAATTTAGATCGACGTTTGGTTAATGATATCAGGAAAAGAAACAGTTGGCCAAGGAAAGCGCTATCATATTCTAATTGCCTCCTTATTTCTCAATATAATCATAATCGCAAACCACCAACGCACCCGAATCCACACAATAAGTCCATTCATTTTGGTGCCAATTGCACTGGCAGTAGCGGTAGTGAGACCACCGAGCTCGAAGATTCGTCTGACATCGTTGATTGTGTGGGAACAGGGCAAGACGACGTAGAGTGCGTGGTCACAGCCGAAGAAACTGGTGGTCCTGGATTGGAACTGGAATTATTAGAACAAGTGCAAGAATGGGCAGTACTGGTCTCTCCCTTCTTCTTTTGGGGTACGGCTATGGTGGCCATGAAGGGGATTCTGCCAAAGACCGGCCCTTTCTTCATCTCAGCCTTCCGTCTAATTCCTGCTGGCTTTCTCCTCGTTGCCTTCGCCGCCTACAGAGGCCGCCCCTTTCCCTCTGGCCTCACCGCTTGGCTTTCTATTGCGCTTTTCGGCCTCGTTGATGCTGCTTGTTTTCAAGGATTTCTTGCTCAAGGTTTGCAGAGGACATCTGCTGGTCTTGGCAGCGTTATAATTGATTCACAACCTTTGACAGTCGCTATACTTGCGGCCTTGTTATTTGGTGAGTCCATTGGCTTCCTTGGAGCTGCAGGGCTTGTTCTTGGCGTCATTGGACTTTTACTTCTTGAGGCACCTTCACTTGCTTTGGATGGAAGTAACTCTTCATTGTGGCAAAGTGGGGAGTGGTGGATGCTTCTCTCAGCTCAGAGCATGGCAGTTGGTACAGTCATGGTGCGTTGGGTTTCCAAATACTCTGATCCTATTATGGCAACTGGATGGGTAGGCTATTCAACTCACTTGATAATAACTTATAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EamA

Protein Analysis

326

Amino Acids

35.05

Weight (kDa)

5.54

Isoelectric Point (pI)

38.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EamA PF00892 137 - 263 2.6e-23 EamA-like transporter family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 674, 975
AccB1I GGYRCC 2 cut(s) 230, 793
AciI CCGC 6 cut(s) 70, 252, 534, 547, 570, 710
AclWI GGATC 1 cut(s) 914
AcoI YGGCCR 3 cut(s) 71, 122, 444
AcsI RAATTY 1 cut(s) 76
AcyI GRCGYC 1 cut(s) 768
AfaI GTAC 3 cut(s) 405, 433, 886
AfeI AGCGCT 1 cut(s) 135
AfiI CCNNNNNNNGG 2 cut(s) 453, 470
AgsI TTSAA 2 cut(s) 620, 954
AjnI CCWGG 2 cut(s) 55, 358
AluBI AGCT 3 cut(s) 271, 749, 866
AluI AGCT 3 cut(s) 271, 749, 866
Alw21I GWGCWC 1 cut(s) 273
Alw26I GTCTC 2 cut(s) 254, 415
AlwI GGATC 1 cut(s) 914
AlwNI CAGNNNCTG 1 cut(s) 23
Aor51HI AGCGCT 1 cut(s) 135
AoxI GGCC 8 cut(s) 71, 122, 444, 472, 544, 562, 595, 711
ApeKI GCWGC 3 cut(s) 608, 666, 749
ApoI RAATTY 1 cut(s) 76
AspLEI GCGC 2 cut(s) 136, 589
AspS9I GGNCC 2 cut(s) 356, 473
AsuHPI GGTGA 2 cut(s) 559, 737
AvaII GGWCC 1 cut(s) 356
BalI TGGCCA 2 cut(s) 124, 446
BanI GGYRCC 2 cut(s) 230, 793
BanII GRGCYC 1 cut(s) 273
Bbv12I GWGCWC 1 cut(s) 273
BbvI GCAGC 3 cut(s) 595, 678, 736
BccI CCATC 2 cut(s) 806, 933
BceAI ACGGC 1 cut(s) 450
BciT130I CCWGG 2 cut(s) 57, 360
BcoDI GTCTC 2 cut(s) 254, 415
BfmI CTRYAG 2 cut(s) 538, 750
BfoI RGCGCY 1 cut(s) 137
BisI GCNGC 7 cut(s) 71, 534, 547, 609, 667, 711, 750
BlsI GCNGC 7 cut(s) 72, 535, 548, 610, 668, 712, 751
BmcAI AGTACT 1 cut(s) 405
Bme1390I CCNGG 2 cut(s) 57, 360
Bme18I GGWCC 1 cut(s) 356
BmgT120I GGNCC 2 cut(s) 356, 473
BmiI GGNNCC 2 cut(s) 232, 795
BmrFI CCNGG 2 cut(s) 57, 360
BmsI GCATC 2 cut(s) 595, 843
BpuEI CTTGAG 2 cut(s) 618, 809
BsaHI GRCGYC 1 cut(s) 768
BsaI GGTCTC 2 cut(s) 254, 415
BsaJI CCNNGG 4 cut(s) 55, 56, 125, 742
Bsc4I CCNNNNNNNGG 2 cut(s) 453, 470
Bse118I RCCGGY 1 cut(s) 470
Bse1I ACTGG 5 cut(s) 247, 355, 375, 411, 940
BseBI CCWGG 2 cut(s) 57, 360
BseDI CCNNGG 4 cut(s) 55, 56, 125, 742
BseGI GGATG 3 cut(s) 817, 858, 944
BseLI CCNNNNNNNGG 2 cut(s) 453, 470
BseMII CTCAG 4 cut(s) 30, 501, 876, 881
BseNI ACTGG 5 cut(s) 247, 355, 375, 411, 940
BseRI GAGGAG 1 cut(s) 509
BseX3I CGGCCG 1 cut(s) 71
BseXI GCAGC 3 cut(s) 595, 678, 736
Bsh1285I CGRYCG 1 cut(s) 74
BshFI GGCC 8 cut(s) 73, 124, 446, 474, 546, 564, 597, 713
BshNI GGYRCC 2 cut(s) 230, 793
BsiEI CGRYCG 1 cut(s) 74
BsiHKAI GWGCWC 1 cut(s) 273
BsiSI CCGG 1 cut(s) 471
BslFI GGGAC 1 cut(s) 73
BslI CCNNNNNNNGG 2 cut(s) 453, 470
BsmAI GTCTC 2 cut(s) 254, 415
BsmFI GGGAC 1 cut(s) 73
BsnI GGCC 8 cut(s) 73, 124, 446, 474, 546, 564, 597, 713
Bso31I GGTCTC 2 cut(s) 254, 415
Bsp1286I GDGCHC 1 cut(s) 273
Bsp143I GATC 2 cut(s) 82, 919
BspACI CCGC 6 cut(s) 70, 252, 534, 547, 570, 710
BspANI GGCC 8 cut(s) 73, 124, 446, 474, 546, 564, 597, 713
BspCNI CTCAG 4 cut(s) 29, 500, 875, 880
BspLI GGNNCC 2 cut(s) 232, 795
BspMAI CTGCAG 1 cut(s) 754
BspPI GGATC 1 cut(s) 914
BspT107I GGYRCC 2 cut(s) 230, 793
BspTNI GGTCTC 2 cut(s) 254, 415
BsrFI RCCGGY 1 cut(s) 470
BsrI ACTGG 5 cut(s) 247, 355, 375, 411, 940
BssAI RCCGGY 1 cut(s) 470
BssECI CCNNGG 4 cut(s) 55, 56, 125, 742
BssMI GATC 2 cut(s) 82, 919
BssNI GRCGYC 1 cut(s) 768
BssT1I CCWWGG 2 cut(s) 125, 742
Bst2UI CCWGG 2 cut(s) 57, 360
Bst4CI ACNGT 5 cut(s) 35, 64, 119, 697, 889
Bst6I CTCTTC 1 cut(s) 829
BstACI GRCGYC 1 cut(s) 768
BstC8I GCNNGC 2 cut(s) 28, 511
BstDEI CTNAG 4 cut(s) 16, 487, 862, 867
BstF5I GGATG 3 cut(s) 817, 858, 944
BstH2I RGCGCY 1 cut(s) 137
BstHHI GCGC 2 cut(s) 136, 589
BstKTI GATC 2 cut(s) 85, 922
BstMAI GTCTC 2 cut(s) 254, 415
BstMBI GATC 2 cut(s) 82, 919
BstMCI CGRYCG 1 cut(s) 74
BstMWI GCNNNNNNNGC 3 cut(s) 443, 533, 707
BstNI CCWGG 2 cut(s) 57, 360
BstSCI CCNGG 2 cut(s) 55, 358
BstSFI CTRYAG 2 cut(s) 538, 750
BstV1I GCAGC 3 cut(s) 595, 678, 736
BstZI CGGCCG 1 cut(s) 71
BsuRI GGCC 8 cut(s) 73, 124, 446, 474, 546, 564, 597, 713
BtsCI GGATG 3 cut(s) 817, 858, 944
BtsIMutI CAGTG 1 cut(s) 240
Cac8I GCNNGC 2 cut(s) 28, 511
CaiI CAGNNNCTG 1 cut(s) 23
CfoI GCGC 2 cut(s) 136, 589
Cfr10I RCCGGY 1 cut(s) 470
Cfr13I GGNCC 2 cut(s) 356, 473
CseI GACGC 1 cut(s) 757
Csp6I GTAC 3 cut(s) 404, 432, 885
CviAII CATG 3 cut(s) 448, 874, 892
CviQI GTAC 3 cut(s) 404, 432, 885
DdeI CTNAG 4 cut(s) 16, 487, 862, 867
DpnI GATC 2 cut(s) 84, 921
DpnII GATC 2 cut(s) 82, 919
EaeI YGGCCR 3 cut(s) 71, 122, 444
EagI CGGCCG 1 cut(s) 71
Eam1104I CTCTTC 1 cut(s) 829
EarI CTCTTC 1 cut(s) 829
Ecl136II GAGCTC 1 cut(s) 271
EclXI CGGCCG 1 cut(s) 71
Eco130I CCWWGG 2 cut(s) 125, 742
Eco24I GRGCYC 1 cut(s) 273
Eco31I GGTCTC 2 cut(s) 254, 415
Eco32I GATATC 1 cut(s) 103
Eco47I GGWCC 1 cut(s) 356
Eco47III AGCGCT 1 cut(s) 135
Eco52I CGGCCG 1 cut(s) 71
Eco53kI GAGCTC 1 cut(s) 271
EcoICRI GAGCTC 1 cut(s) 271
EcoRII CCWGG 2 cut(s) 55, 358
EcoRV GATATC 1 cut(s) 103
EcoT14I CCWWGG 2 cut(s) 125, 742
EcoT38I GRGCYC 1 cut(s) 273
ErhI CCWWGG 2 cut(s) 125, 742
FaeI CATG 3 cut(s) 451, 877, 895
FalI AAGNNNNNCTT 4 cut(s) 612, 644, 808, 840
FaqI GGGAC 1 cut(s) 73
FatI CATG 3 cut(s) 447, 873, 891
Fnu4HI GCNGC 7 cut(s) 71, 534, 547, 609, 667, 711, 750
FokI GGATG 3 cut(s) 824, 865, 951
FriOI GRGCYC 1 cut(s) 273
Fsp4HI GCNGC 7 cut(s) 71, 534, 547, 609, 667, 711, 750
GlaI GCGC 2 cut(s) 135, 588
GluI GCNGC 7 cut(s) 71, 534, 547, 609, 667, 711, 750
HaeII RGCGCY 1 cut(s) 137
HaeIII GGCC 8 cut(s) 73, 124, 446, 474, 546, 564, 597, 713
HapII CCGG 1 cut(s) 471
HgaI GACGC 1 cut(s) 757
HhaI GCGC 2 cut(s) 136, 589
Hin1I GRCGYC 1 cut(s) 768
Hin1II CATG 3 cut(s) 451, 877, 895
Hin6I GCGC 2 cut(s) 134, 587
HinP1I GCGC 2 cut(s) 134, 587
HinfI GANTC 5 cut(s) 202, 278, 457, 680, 728
HpaII CCGG 1 cut(s) 471
HphI GGTGA 2 cut(s) 559, 737
Hpy188I TCNGA 3 cut(s) 286, 870, 919
Hpy188III TCNNGA 2 cut(s) 106, 788
Hpy99I CGWCG 2 cut(s) 90, 323
HpyAV CCTTC 5 cut(s) 427, 445, 502, 538, 807
HpyCH4III ACNGT 5 cut(s) 35, 64, 119, 697, 889
HpyCH4IV ACGT 2 cut(s) 88, 321
HpyCH4V TGCA 4 cut(s) 240, 391, 643, 752
HpyF10VI GCNNNNNNNGC 3 cut(s) 443, 533, 707
HpyF3I CTNAG 4 cut(s) 16, 487, 862, 867
HpySE526I ACGT 2 cut(s) 88, 321
Hsp92I GRCGYC 1 cut(s) 768
Hsp92II CATG 3 cut(s) 451, 877, 895
HspAI GCGC 2 cut(s) 134, 587
Kzo9I GATC 2 cut(s) 82, 919
LmnI GCTCC 1 cut(s) 746
Lsp1109I GCAGC 3 cut(s) 595, 678, 736
LweI GCATC 2 cut(s) 595, 843
MaeII ACGT 2 cut(s) 88, 321
MaeIII GTNAC 2 cut(s) 334, 818
MalI GATC 2 cut(s) 84, 921
MboI GATC 2 cut(s) 82, 919
MboII GAAGA 5 cut(s) 287, 356, 412, 472, 816
MfeI CAATTG 1 cut(s) 235
MhlI GDGCHC 1 cut(s) 273
MlsI TGGCCA 2 cut(s) 124, 446
MluCI AATT 7 cut(s) 76, 148, 235, 374, 501, 675, 976
MluNI TGGCCA 2 cut(s) 124, 446
MlyI GAGTC 1 cut(s) 737
MnlI CCTC 9 cut(s) 25, 164, 530, 536, 568, 575, 608, 639, 784
Mox20I TGGCCA 2 cut(s) 124, 446
MscI TGGCCA 2 cut(s) 124, 446
MseI TTAA 1 cut(s) 96
MslI CAYNNNNRTG 1 cut(s) 228
Msp20I TGGCCA 2 cut(s) 124, 446
MspI CCGG 1 cut(s) 471
MspR9I CCNGG 2 cut(s) 57, 360
MunI CAATTG 1 cut(s) 235
MvaI CCWGG 2 cut(s) 57, 360
MwoI GCNNNNNNNGC 3 cut(s) 443, 533, 707
NdeII GATC 2 cut(s) 82, 919
NlaIII CATG 3 cut(s) 451, 877, 895
NlaIV GGNNCC 2 cut(s) 232, 795
NmuCI GTSAC 1 cut(s) 334
PasI CCCWGGG 1 cut(s) 56
PcsI WCGNNNNNNNCGW 1 cut(s) 327
PfeI GAWTC 4 cut(s) 202, 278, 457, 680
PfoI TCCNGGA 1 cut(s) 358
PkrI GCNGC 7 cut(s) 72, 535, 548, 610, 668, 712, 751
PleI GAGTC 1 cut(s) 736
PpsI GAGTC 1 cut(s) 736
PsiI TTATAA 2 cut(s) 674, 975
Psp124BI GAGCTC 1 cut(s) 273
Psp6I CCWGG 2 cut(s) 55, 358
PspGI CCWGG 2 cut(s) 55, 358
PspN4I GGNNCC 2 cut(s) 232, 795
PspPI GGNCC 2 cut(s) 356, 473
PstI CTGCAG 1 cut(s) 754
PstNI CAGNNNCTG 1 cut(s) 23
RsaI GTAC 3 cut(s) 405, 433, 886
RsaNI GTAC 3 cut(s) 404, 432, 885
RseI CAYNNNNRTG 1 cut(s) 228
SacI GAGCTC 1 cut(s) 273
SaqAI TTAA 1 cut(s) 96
SatI GCNGC 7 cut(s) 71, 534, 547, 609, 667, 711, 750
Sau3AI GATC 2 cut(s) 82, 919
Sau96I GGNCC 2 cut(s) 356, 473
ScaI AGTACT 1 cut(s) 405
SchI GAGTC 1 cut(s) 737
ScrFI CCNGG 2 cut(s) 57, 360
SduI GDGCHC 1 cut(s) 273
SetI ASST 8 cut(s) 91, 273, 324, 640, 691, 751, 799, 868
SfaNI GCATC 2 cut(s) 595, 843
SfcI CTRYAG 2 cut(s) 538, 750
SinI GGWCC 1 cut(s) 356
SmiMI CAYNNNNRTG 1 cut(s) 228
SmlI CTYRAG 2 cut(s) 633, 788
SmoI CTYRAG 2 cut(s) 633, 788
Sse9I AATT 7 cut(s) 76, 148, 235, 374, 501, 675, 976
SsiI CCGC 6 cut(s) 70, 252, 534, 547, 570, 710
SstI GAGCTC 1 cut(s) 273
StyD4I CCNGG 2 cut(s) 55, 358
StyI CCWWGG 2 cut(s) 125, 742
TaaI ACNGT 5 cut(s) 35, 64, 119, 697, 889
TaiI ACGT 2 cut(s) 91, 324
TaqI TCGA 2 cut(s) 85, 273
TasI AATT 7 cut(s) 76, 148, 235, 374, 501, 675, 976
TatI WGTACW 1 cut(s) 403
TauI GCSGC 4 cut(s) 73, 536, 549, 713
TfiI GAWTC 4 cut(s) 202, 278, 457, 680
Tru1I TTAA 1 cut(s) 96
Tru9I TTAA 1 cut(s) 96
TscAI CASTG 1 cut(s) 247
TseFI GTSAC 1 cut(s) 334
TseI GCWGC 3 cut(s) 608, 666, 749
Tsp45I GTSAC 1 cut(s) 334
TspDTI ATGAA 4 cut(s) 212, 464, 472, 816
TspGWI ACGGA 1 cut(s) 485
TspRI CASTG 1 cut(s) 247
VpaK11BI GGWCC 1 cut(s) 356
XapI RAATTY 1 cut(s) 76
ZrmI AGTACT 1 cut(s) 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.