FvH4_6g01200

Serine incorporator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0004619
Physical Location & Seq
Forward (+)
653050 .. 656701
3652 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g01200.t1

Sequence Viewer

Length: 1287 bp
ATGGTCCTTAATGATTCTCTCAACGTCTTGACAATGTCAGTGATAACTTCATTTCCTTCCTTTCCTTCCTTTCCTTCTTCCACAATGGATGACTACTATAAACGGAGCGGTCAAATTCCGGCTTTTGGGGACTGGGATTATGCCAATGAGCTGCCAATCACTCAGTATTTTGAGTGTGCAAGGCAAGCTGGTTTGATCAGGTTCAGCTCTTCTTCTGGTGAGAGCGATGTTCCGGCAGCCTATCTGCATGCTGACTTGTATGCTGTTGATGTTAAGAAGATGAAGAAGAAGCCTTCCCGGGTTGTTGTTGCTCCCAATTCTCGAAAGACAACTGCAAGAGGGAGAGAAAAGAGATGCACTCATAACCCGCATGTCAAGGAGCAAAAGAAGCAGCAGCATCAAGCTAAGGTCCATGACGGAGTGACTGAACTGGCAAAGCTACACTACCAGCAGTATCACACTCGCCGCAACGATGCCGTTTTGCGACCAAGGAGGCCTCCCAAGCCGGTTGATGAAGACCTCTACAAGATACCACCTGAGCTCCTTCACTCTACCAAGCGGAGGCAGAAGGATCCCCGTGACAGTATCCACCATGGTGGATGGATGATGAAAGTCACTTGCTGGTTCCTCTTGGTGCTCTTCTCCTTGTATTTCGTGCCAAATGGGTTTATTAGTTTCTATGAGACAATTGCAAAGTTTGGCTCAGGATTCTTTCTTCTCGTACAAGTTGTGCTTCTGTTGGATTTTGTTCATGGATGGAACAACAAATGGGTTTCATATGATGAGCAGTTCTGGTATGTTGCTCTTTTTGTTGTTTCTCTAGTTTGTTATCTGGCAACATTCGTCTTCTGTGGACTTCTTTTCCATTTGTTCACGCCATCTGGACATGACTGCAGACTCAATACCTTCTTCATCGTAATGACTCTGATATTCGTTTTCGTTTTTCTTATAGTGGCACTGCATCCTACTGTAGGTGGCAGCATTTTACCCGCGTCAGTTATATCTGTGTACTGTACATATCTCTGCTATAGTGCACTTGCTAGTGAGCCGAGGGACTATGAGTGCAATGGTCTCCACAAGCATTCTAAAGCTGTTTCCACTGGGACTCTTACCTTAGGTTTGCTTACCACTGTTCTTTCTGTGGTTTATTCTGCTGTGCGTGCTGGATCTTCCACAACTCTTTTGTCCCCGCCTAGTTCACCTCGTGCAGGTGCTGGGAAACCACTGCTCCCATTGGATAAGCCGGAAGAACGTGAAGAAAATGAGAAAGCCAAGTCAGCTGATTGA

Protein Analysis

429

Amino Acids

48.29

Weight (kDa)

9.03

Isoelectric Point (pI)

48.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Serinc PF03348 187 - 412 2.7e-69 Serine incorporator (Serinc)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1199
Acc36I ACCTGC 1 cut(s) 1199
AccBSI CCGCTC 1 cut(s) 108
AccII CGCG 1 cut(s) 992
AciI CCGC 6 cut(s) 108, 368, 466, 559, 990, 1190
AclWI GGATC 3 cut(s) 566, 579, 1174
AcsI RAATTY 1 cut(s) 114
AdeI CACNNNGTG 1 cut(s) 1205
AfaI GTAC 3 cut(s) 723, 1010, 1015
AfiI CCNNNNNNNGG 4 cut(s) 125, 561, 971, 1208
AleI CACNNNNGTG 1 cut(s) 594
AluBI AGCT 8 cut(s) 151, 188, 207, 404, 439, 541, 1091, 1280
AluI AGCT 8 cut(s) 151, 188, 207, 404, 439, 541, 1091, 1280
Alw21I GWGCWC 3 cut(s) 543, 639, 1036
Alw26I GTCTC 2 cut(s) 677, 1076
Alw44I GTGCAC 1 cut(s) 1032
AlwI GGATC 3 cut(s) 566, 579, 1174
AlwNI CAGNNNCTG 1 cut(s) 1214
Ama87I CYCGRG 1 cut(s) 297
AoxI GGCC 1 cut(s) 494
ApaLI GTGCAC 1 cut(s) 1032
ApeKI GCWGC 5 cut(s) 151, 236, 391, 394, 978
ApoI RAATTY 1 cut(s) 114
AspS9I GGNCC 2 cut(s) 4, 409
AsuC2I CCSGG 2 cut(s) 298, 299
AsuHPI GGTGA 2 cut(s) 230, 1191
AvaI CYCGRG 1 cut(s) 297
AvaII GGWCC 2 cut(s) 4, 409
AxyI CCTNAGG 1 cut(s) 1114
BaeGI GKGCMC 1 cut(s) 1036
BamHI GGATCC 1 cut(s) 571
BanII GRGCYC 1 cut(s) 543
BauI CACGAG 1 cut(s) 1203
BbsI GAAGAC 2 cut(s) 522, 838
Bbv12I GWGCWC 3 cut(s) 543, 639, 1036
BbvI GCAGC 5 cut(s) 138, 248, 403, 406, 990
BccI CCATC 3 cut(s) 594, 750, 886
BceAI ACGGC 1 cut(s) 461
BciVI GTATCC 1 cut(s) 596
BclI TGATCA 1 cut(s) 195
BcnI CCSGG 2 cut(s) 298, 299
BcoDI GTCTC 2 cut(s) 677, 1076
BfaI CTAG 3 cut(s) 821, 1041, 1194
BfmI CTRYAG 3 cut(s) 892, 969, 1027
BfuAI ACCTGC 1 cut(s) 1199
BfuI GTATCC 1 cut(s) 596
BisI GCNGC 6 cut(s) 152, 237, 392, 395, 466, 979
BlsI GCNGC 6 cut(s) 153, 238, 393, 396, 467, 980
Bme1390I CCNGG 2 cut(s) 298, 299
Bme18I GGWCC 2 cut(s) 4, 409
BmeT110I CYCGRG 1 cut(s) 297
BmgT120I GGNCC 2 cut(s) 4, 409
BmiI GGNNCC 2 cut(s) 573, 626
BmrFI CCNGG 2 cut(s) 298, 299
BmrI ACTGGG 2 cut(s) 142, 1110
BmsI GCATC 4 cut(s) 344, 406, 463, 970
BmuI ACTGGG 2 cut(s) 142, 1110
BpiI GAAGAC 2 cut(s) 522, 838
BplI GAGNNNNNCTC 2 cut(s) 343, 375
Bpu10I CCTNAGC 3 cut(s) 405, 537, 703
BpuMI CCSGG 2 cut(s) 298, 299
BsaI GGTCTC 1 cut(s) 1076
BsaJI CCNNGG 4 cut(s) 297, 488, 592, 1049
BsaXI ACNNNNNCTCC 4 cut(s) 525, 555, 1212, 1242
Bsc4I CCNNNNNNNGG 4 cut(s) 125, 561, 971, 1208
Bse118I RCCGGY 1 cut(s) 505
Bse1I ACTGG 3 cut(s) 137, 435, 1105
Bse21I CCTNAGG 1 cut(s) 1114
Bse3DI GCAATG 1 cut(s) 1072
BseDI CCNNGG 4 cut(s) 297, 488, 592, 1049
BseGI GGATG 5 cut(s) 94, 605, 609, 761, 961
BseLI CCNNNNNNNGG 4 cut(s) 125, 561, 971, 1208
BseMI GCAATG 1 cut(s) 1072
BseMII CTCAG 3 cut(s) 176, 528, 717
BseNI ACTGG 3 cut(s) 137, 435, 1105
BseSI GKGCMC 1 cut(s) 1036
BseXI GCAGC 5 cut(s) 138, 248, 403, 406, 990
BseYI CCCAGC 1 cut(s) 1214
BsgI GTGCAG 1 cut(s) 1227
Bsh1236I CGCG 1 cut(s) 992
BshFI GGCC 1 cut(s) 496
BsiHKAI GWGCWC 3 cut(s) 543, 639, 1036
BsiHKCI CYCGRG 1 cut(s) 297
BsiSI CCGG 5 cut(s) 119, 233, 298, 506, 1244
BslFI GGGAC 4 cut(s) 143, 1067, 1117, 1171
BslI CCNNNNNNNGG 4 cut(s) 125, 561, 971, 1208
BsmAI GTCTC 2 cut(s) 677, 1076
BsmFI GGGAC 4 cut(s) 143, 1067, 1117, 1171
BsmI GAATGC 1 cut(s) 1081
BsnI GGCC 1 cut(s) 496
Bso31I GGTCTC 1 cut(s) 1076
BsoBI CYCGRG 1 cut(s) 297
Bsp1286I GDGCHC 3 cut(s) 543, 639, 1036
Bsp1407I TGTACA 1 cut(s) 1013
Bsp143I GATC 3 cut(s) 195, 571, 1166
Bsp19I CCATGG 1 cut(s) 592
BspACI CCGC 6 cut(s) 108, 368, 466, 559, 990, 1190
BspANI GGCC 1 cut(s) 496
BspCNI CTCAG 3 cut(s) 175, 529, 716
BspFNI CGCG 1 cut(s) 992
BspLI GGNNCC 2 cut(s) 573, 626
BspMAI CTGCAG 1 cut(s) 896
BspMI ACCTGC 1 cut(s) 1199
BspPI GGATC 3 cut(s) 566, 579, 1174
BspQI GCTCTTC 2 cut(s) 214, 644
BspTNI GGTCTC 1 cut(s) 1076
BsrBI CCGCTC 1 cut(s) 108
BsrDI GCAATG 1 cut(s) 1072
BsrFI RCCGGY 1 cut(s) 505
BsrGI TGTACA 1 cut(s) 1013
BsrI ACTGG 3 cut(s) 137, 435, 1105
BssAI RCCGGY 1 cut(s) 505
BssECI CCNNGG 4 cut(s) 297, 488, 592, 1049
BssMI GATC 3 cut(s) 195, 571, 1166
BssSI CACGAG 1 cut(s) 1203
BssT1I CCWWGG 2 cut(s) 488, 592
Bst2BI CACGAG 1 cut(s) 1203
Bst4CI ACNGT 4 cut(s) 584, 970, 1013, 1132
Bst6I CTCTTC 2 cut(s) 214, 644
BstAUI TGTACA 1 cut(s) 1013
BstC8I GCNNGC 3 cut(s) 186, 249, 1161
BstDEI CTNAG 5 cut(s) 162, 405, 537, 703, 1114
BstDSI CCRYGG 1 cut(s) 592
BstENI CCTNNNNNAGG 2 cut(s) 969, 1206
BstF5I GGATG 5 cut(s) 94, 605, 609, 761, 961
BstFNI CGCG 1 cut(s) 992
BstKTI GATC 3 cut(s) 198, 574, 1169
BstMAI GTCTC 2 cut(s) 677, 1076
BstMBI GATC 3 cut(s) 195, 571, 1166
BstMWI GCNNNNNNNGC 5 cut(s) 185, 388, 502, 1160, 1277
BstNSI RCATGY 2 cut(s) 251, 374
BstSCI CCNGG 2 cut(s) 296, 297
BstSFI CTRYAG 3 cut(s) 892, 969, 1027
BstSLI GKGCMC 1 cut(s) 1036
BstUI CGCG 1 cut(s) 992
BstV1I GCAGC 5 cut(s) 138, 248, 403, 406, 990
BstV2I GAAGAC 2 cut(s) 522, 838
BstX2I RGATCY 2 cut(s) 571, 1166
BstXI CCANNNNNNTGG 1 cut(s) 596
BstYI RGATCY 2 cut(s) 571, 1166
Bsu36I CCTNAGG 1 cut(s) 1114
BsuI GTATCC 1 cut(s) 596
BsuRI GGCC 1 cut(s) 496
BtgI CCRYGG 1 cut(s) 592
BtgZI GCGATG 1 cut(s) 240
BtsCI GGATG 5 cut(s) 94, 605, 609, 761, 961
BtsI GCAGTG 2 cut(s) 956, 1223
BtsIMutI CAGTG 5 cut(s) 45, 956, 1098, 1128, 1223
BveI ACCTGC 1 cut(s) 1199
Cac8I GCNNGC 3 cut(s) 186, 249, 1161
CaiI CAGNNNCTG 1 cut(s) 1214
Cfr10I RCCGGY 1 cut(s) 505
Cfr13I GGNCC 2 cut(s) 4, 409
Cfr9I CCCGGG 1 cut(s) 297
CseI GACGC 1 cut(s) 981
Csp6I GTAC 3 cut(s) 722, 1009, 1014
CviAII CATG 6 cut(s) 248, 371, 413, 593, 752, 887
CviQI GTAC 3 cut(s) 722, 1009, 1014
DdeI CTNAG 5 cut(s) 162, 405, 537, 703, 1114
DpnI GATC 3 cut(s) 197, 573, 1168
DpnII GATC 3 cut(s) 195, 571, 1166
DraIII CACNNNGTG 1 cut(s) 1205
Eam1104I CTCTTC 2 cut(s) 214, 644
EarI CTCTTC 2 cut(s) 214, 644
Ecl136II GAGCTC 1 cut(s) 541
Eco130I CCWWGG 2 cut(s) 488, 592
Eco147I AGGCCT 1 cut(s) 496
Eco24I GRGCYC 1 cut(s) 543
Eco31I GGTCTC 1 cut(s) 1076
Eco47I GGWCC 2 cut(s) 4, 409
Eco53kI GAGCTC 1 cut(s) 541
Eco81I CCTNAGG 1 cut(s) 1114
Eco88I CYCGRG 1 cut(s) 297
EcoICRI GAGCTC 1 cut(s) 541
EcoNI CCTNNNNNAGG 2 cut(s) 969, 1206
EcoT14I CCWWGG 2 cut(s) 488, 592
EcoT38I GRGCYC 1 cut(s) 543
ErhI CCWWGG 2 cut(s) 488, 592
FaeI CATG 6 cut(s) 251, 374, 416, 596, 755, 890
FalI AAGNNNNNCTT 2 cut(s) 717, 749
FaqI GGGAC 4 cut(s) 143, 1067, 1117, 1171
FatI CATG 6 cut(s) 247, 370, 412, 592, 751, 886
FauI CCCGC 3 cut(s) 375, 997, 1197
FauNDI CATATG 1 cut(s) 778
FbaI TGATCA 1 cut(s) 195
Fnu4HI GCNGC 6 cut(s) 152, 237, 392, 395, 466, 979
FokI GGATG 5 cut(s) 101, 612, 616, 768, 948
FriOI GRGCYC 1 cut(s) 543
Fsp4HI GCNGC 6 cut(s) 152, 237, 392, 395, 466, 979
FspBI CTAG 3 cut(s) 821, 1041, 1194
GluI GCNGC 6 cut(s) 152, 237, 392, 395, 466, 979
GsaI CCCAGC 1 cut(s) 1218
HaeIII GGCC 1 cut(s) 496
HapII CCGG 5 cut(s) 119, 233, 298, 506, 1244
HgaI GACGC 1 cut(s) 981
Hin1II CATG 6 cut(s) 251, 374, 416, 596, 755, 890
HinfI GANTC 5 cut(s) 14, 708, 897, 922, 1105
HpaII CCGG 5 cut(s) 119, 233, 298, 506, 1244
HphI GGTGA 2 cut(s) 230, 1191
Hpy166II GTNNAC 5 cut(s) 854, 873, 1009, 1034, 1199
Hpy188I TCNGA 1 cut(s) 927
Hpy188III TCNNGA 4 cut(s) 28, 321, 705, 882
Hpy8I GTNNAC 5 cut(s) 854, 873, 1009, 1034, 1199
HpyAV CCTTC 7 cut(s) 66, 75, 84, 303, 554, 562, 916
HpyCH4III ACNGT 4 cut(s) 584, 970, 1013, 1132
HpyCH4IV ACGT 2 cut(s) 24, 1252
HpyF10VI GCNNNNNNNGC 5 cut(s) 185, 388, 502, 1160, 1277
HpyF3I CTNAG 5 cut(s) 162, 405, 537, 703, 1114
HpySE526I ACGT 2 cut(s) 24, 1252
Hsp92II CATG 6 cut(s) 251, 374, 416, 596, 755, 890
Ksp22I TGATCA 1 cut(s) 195
Kzo9I GATC 3 cut(s) 195, 571, 1166
LguI GCTCTTC 2 cut(s) 214, 644
LmnI GCTCC 5 cut(s) 105, 316, 379, 546, 1233
Lsp1109I GCAGC 5 cut(s) 138, 248, 403, 406, 990
LweI GCATC 4 cut(s) 344, 406, 463, 970
MaeI CTAG 3 cut(s) 821, 1041, 1194
MaeII ACGT 2 cut(s) 24, 1252
MaeIII GTNAC 3 cut(s) 421, 578, 613
MalI GATC 3 cut(s) 197, 573, 1168
MbiI CCGCTC 1 cut(s) 108
MboI GATC 3 cut(s) 195, 571, 1166
MfeI CAATTG 1 cut(s) 687
MflI RGATCY 2 cut(s) 571, 1166
MhlI GDGCHC 3 cut(s) 543, 639, 1036
MluCI AATT 3 cut(s) 114, 316, 687
MlyI GAGTC 3 cut(s) 891, 916, 1099
MmeI TCCRAC 1 cut(s) 720
MnlI CCTC 8 cut(s) 332, 486, 507, 530, 555, 638, 1044, 1212
MseI TTAA 2 cut(s) 9, 273
MslI CAYNNNNRTG 2 cut(s) 594, 917
MspA1I CMGCKG 1 cut(s) 1280
MspI CCGG 5 cut(s) 119, 233, 298, 506, 1244
MspR9I CCNGG 2 cut(s) 298, 299
MunI CAATTG 1 cut(s) 687
Mva1269I GAATGC 1 cut(s) 1081
MvnI CGCG 1 cut(s) 992
MwoI GCNNNNNNNGC 5 cut(s) 185, 388, 502, 1160, 1277
NciI CCSGG 2 cut(s) 298, 299
NcoI CCATGG 1 cut(s) 592
NdeI CATATG 1 cut(s) 778
NdeII GATC 3 cut(s) 195, 571, 1166
NlaIII CATG 6 cut(s) 251, 374, 416, 596, 755, 890
NlaIV GGNNCC 2 cut(s) 573, 626
NmeAIII GCCGAG 1 cut(s) 1074
NmuCI GTSAC 3 cut(s) 421, 578, 613
NspI RCATGY 2 cut(s) 251, 374
OliI CACNNNNGTG 1 cut(s) 594
PaeI GCATGC 1 cut(s) 251
PaqCI CACCTGC 1 cut(s) 1199
PceI AGGCCT 1 cut(s) 496
PciSI GCTCTTC 2 cut(s) 214, 644
PctI GAATGC 1 cut(s) 1081
PfeI GAWTC 2 cut(s) 14, 708
PflFI GACNNNGTC 1 cut(s) 34
PkrI GCNGC 6 cut(s) 153, 238, 393, 396, 467, 980
PleI GAGTC 3 cut(s) 891, 916, 1099
PpsI GAGTC 3 cut(s) 891, 916, 1099
Psp124BI GAGCTC 1 cut(s) 543
PspFI CCCAGC 1 cut(s) 1214
PspN4I GGNNCC 2 cut(s) 573, 626
PspPI GGNCC 2 cut(s) 4, 409
PstI CTGCAG 1 cut(s) 896
PstNI CAGNNNCTG 1 cut(s) 1214
PsuI RGATCY 2 cut(s) 571, 1166
PsyI GACNNNGTC 1 cut(s) 34
PvuII CAGCTG 1 cut(s) 1280
RsaI GTAC 3 cut(s) 723, 1010, 1015
RsaNI GTAC 3 cut(s) 722, 1009, 1014
RseI CAYNNNNRTG 2 cut(s) 594, 917
SacI GAGCTC 1 cut(s) 543
SapI GCTCTTC 2 cut(s) 214, 644
SaqAI TTAA 2 cut(s) 9, 273
SatI GCNGC 6 cut(s) 152, 237, 392, 395, 466, 979
Sau3AI GATC 3 cut(s) 195, 571, 1166
Sau96I GGNCC 2 cut(s) 4, 409
SchI GAGTC 3 cut(s) 891, 916, 1099
ScrFI CCNGG 2 cut(s) 298, 299
SduI GDGCHC 3 cut(s) 543, 639, 1036
SfaNI GCATC 4 cut(s) 344, 406, 463, 970
SfcI CTRYAG 3 cut(s) 892, 969, 1027
SinI GGWCC 2 cut(s) 4, 409
SmaI CCCGGG 1 cut(s) 299
SmiMI CAYNNNNRTG 2 cut(s) 594, 917
SphI GCATGC 1 cut(s) 251
Sse9I AATT 3 cut(s) 114, 316, 687
SseBI AGGCCT 1 cut(s) 496
SsiI CCGC 6 cut(s) 108, 368, 466, 559, 990, 1190
SspMI CTAG 3 cut(s) 821, 1041, 1194
SstI GAGCTC 1 cut(s) 543
StuI AGGCCT 1 cut(s) 496
StyD4I CCNGG 2 cut(s) 296, 297
StyI CCWWGG 2 cut(s) 488, 592
TaaI ACNGT 4 cut(s) 584, 970, 1013, 1132
TaiI ACGT 2 cut(s) 27, 1255
TaqI TCGA 1 cut(s) 322
TasI AATT 3 cut(s) 114, 316, 687
TatI WGTACW 2 cut(s) 1008, 1013
TauI GCSGC 1 cut(s) 468
TfiI GAWTC 2 cut(s) 14, 708
Tru1I TTAA 2 cut(s) 9, 273
Tru9I TTAA 2 cut(s) 9, 273
TscAI CASTG 5 cut(s) 45, 963, 1105, 1135, 1230
TseFI GTSAC 3 cut(s) 421, 578, 613
TseI GCWGC 5 cut(s) 151, 236, 391, 394, 978
Tsp45I GTSAC 3 cut(s) 421, 578, 613
TspDTI ATGAA 7 cut(s) 39, 296, 528, 623, 740, 765, 901
TspGWI ACGGA 2 cut(s) 118, 432
TspMI CCCGGG 1 cut(s) 297
TspRI CASTG 5 cut(s) 45, 963, 1105, 1135, 1230
Tth111I GACNNNGTC 1 cut(s) 34
VneI GTGCAC 1 cut(s) 1032
VpaK11BI GGWCC 2 cut(s) 4, 409
XagI CCTNNNNNAGG 2 cut(s) 969, 1206
XapI RAATTY 1 cut(s) 114
XceI RCATGY 2 cut(s) 251, 374
XmaI CCCGGG 1 cut(s) 297
XspI CTAG 3 cut(s) 821, 1041, 1194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.