pycom12g23250

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0004619
Physical Location & Seq
Reverse (-)
23898736 .. 23900843
2108 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g23250.3

Sequence Viewer

Length: 555 bp
ATGGATGACTATTACAAGAGGAGTGGGCAGATTCCGGCGTTTGGGGACTGGGATTATGCAAACGAACTGCCAATCACTCAGTATTTCGAGTGTGCAAGACAAGCTGGTTTGATCAGATTCAGCTCTTCCTCCGGCGAGAGTACTGATCATATGCGCCCTGACTTGTATGCTGTTCCTGTCGATCACTTCAAGAAGCCTTCTCGCAACGTTGCTCCTCCTCGAAAGGCAACAGCAGGAGGAGGAGGAGGAGGAGGTGGTGGGCGAGGGAGGGAAAAGCGAGGGCAGCGTGCGCATGTTATGGAGCAAAAGAGGCAGCAATCGAAGGGGACAAAAGTCTGTGACGTGACGGAACCGCCAACTAAACCCTATCACTATCAACTCCATGTTCAACAACCCAATCGCACACACCTCCACCACCACAAGAACGACACCGTTTCAAGACCCAAGCCTGTCGACGAAGATCTCTACAAGATACCCCCTGAGCTCCTCCACACCACCAAGCGGAAGAAAATGCTGGGATTGTTTGCTTGCTTTGTGCCTGCTTGTGCTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

185

Amino Acids

20.68

Weight (kDa)

9.53

Isoelectric Point (pI)

64.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 291
AccI GTMKAC 1 cut(s) 453
AciI CCGC 2 cut(s) 353, 502
AclI AACGTT 1 cut(s) 207
AfaI GTAC 1 cut(s) 142
AfiI CCNNNNNNNGG 2 cut(s) 41, 501
AgsI TTSAA 3 cut(s) 190, 389, 438
AjiI CACGTC 1 cut(s) 343
AluBI AGCT 3 cut(s) 104, 123, 484
AluI AGCT 3 cut(s) 104, 123, 484
Alw21I GWGCWC 1 cut(s) 486
ApeKI GCWGC 2 cut(s) 283, 313
AspLEI GCGC 2 cut(s) 156, 292
BanII GRGCYC 1 cut(s) 486
Bbv12I GWGCWC 1 cut(s) 486
BbvI GCAGC 2 cut(s) 295, 325
BclI TGATCA 2 cut(s) 111, 145
BglII AGATCT 1 cut(s) 460
BisI GCNGC 2 cut(s) 284, 314
BlsI GCNGC 2 cut(s) 285, 315
BmcAI AGTACT 1 cut(s) 142
BmgBI CACGTC 1 cut(s) 343
BmiI GGNNCC 1 cut(s) 351
BmrI ACTGGG 1 cut(s) 58
BmuI ACTGGG 1 cut(s) 58
BoxI GACNNNNGTC 1 cut(s) 332
Bpu10I CCTNAGC 1 cut(s) 480
Bsc4I CCNNNNNNNGG 2 cut(s) 41, 501
Bse1I ACTGG 1 cut(s) 53
BseGI GGATG 1 cut(s) 10
BseLI CCNNNNNNNGG 2 cut(s) 41, 501
BseMII CTCAG 2 cut(s) 92, 471
BseNI ACTGG 1 cut(s) 53
BseRI GAGGAG 9 cut(s) 34, 204, 207, 252, 255, 258, 261, 264, 476
BseXI GCAGC 2 cut(s) 295, 325
BseYI CCCAGC 1 cut(s) 514
BsiHKAI GWGCWC 1 cut(s) 486
BsiSI CCGG 2 cut(s) 35, 132
BslFI GGGAC 2 cut(s) 59, 340
BslI CCNNNNNNNGG 2 cut(s) 41, 501
BsmFI GGGAC 2 cut(s) 59, 340
Bsp1286I GDGCHC 1 cut(s) 486
Bsp143I GATC 4 cut(s) 111, 145, 181, 460
BspACI CCGC 2 cut(s) 353, 502
BspCNI CTCAG 2 cut(s) 91, 472
BspHI TCATGA 1 cut(s) 551
BspLI GGNNCC 1 cut(s) 351
BspQI GCTCTTC 1 cut(s) 130
BsrI ACTGG 1 cut(s) 53
BssMI GATC 4 cut(s) 111, 145, 181, 460
Bst4CI ACNGT 1 cut(s) 433
Bst6I CTCTTC 1 cut(s) 130
BstC8I GCNNGC 3 cut(s) 288, 529, 540
BstDEI CTNAG 2 cut(s) 78, 480
BstF5I GGATG 1 cut(s) 10
BstHHI GCGC 2 cut(s) 156, 292
BstKTI GATC 4 cut(s) 114, 148, 184, 463
BstMBI GATC 4 cut(s) 111, 145, 181, 460
BstMWI GCNNNNNNNGC 4 cut(s) 101, 283, 289, 310
BstNSI RCATGY 1 cut(s) 296
BstPAI GACNNNNGTC 1 cut(s) 332
BstV1I GCAGC 2 cut(s) 295, 325
BstX2I RGATCY 1 cut(s) 460
BstYI RGATCY 1 cut(s) 460
BtrI CACGTC 1 cut(s) 343
BtsCI GGATG 1 cut(s) 10
Cac8I GCNNGC 3 cut(s) 288, 529, 540
CciI TCATGA 1 cut(s) 551
CfoI GCGC 2 cut(s) 156, 292
Csp6I GTAC 1 cut(s) 141
CspCI CAANNNNNGTGG 2 cut(s) 4, 39
CviAII CATG 3 cut(s) 293, 383, 552
CviJI RGCY 5 cut(s) 104, 123, 196, 448, 484
CviKI_1 RGCY 5 cut(s) 104, 123, 196, 448, 484
CviQI GTAC 1 cut(s) 141
DdeI CTNAG 2 cut(s) 78, 480
DpnI GATC 4 cut(s) 113, 147, 183, 462
DpnII GATC 4 cut(s) 111, 145, 181, 460
Eam1104I CTCTTC 1 cut(s) 130
EarI CTCTTC 1 cut(s) 130
Ecl136II GAGCTC 1 cut(s) 484
Eco24I GRGCYC 1 cut(s) 486
Eco53kI GAGCTC 1 cut(s) 484
EcoICRI GAGCTC 1 cut(s) 484
EcoT38I GRGCYC 1 cut(s) 486
FaeI CATG 3 cut(s) 296, 386, 555
FaiI YATR 8 cut(s) 57, 150, 152, 168, 294, 299, 384, 553
FaqI GGGAC 2 cut(s) 59, 340
FatI CATG 3 cut(s) 292, 382, 551
FauNDI CATATG 1 cut(s) 150
FbaI TGATCA 2 cut(s) 111, 145
FblI GTMKAC 1 cut(s) 453
Fnu4HI GCNGC 2 cut(s) 284, 314
FokI GGATG 1 cut(s) 17
FriOI GRGCYC 1 cut(s) 486
Fsp4HI GCNGC 2 cut(s) 284, 314
FspAI RTGCGCAY 1 cut(s) 291
FspI TGCGCA 1 cut(s) 291
GlaI GCGC 2 cut(s) 155, 291
GluI GCNGC 2 cut(s) 284, 314
GsaI CCCAGC 1 cut(s) 518
HapII CCGG 2 cut(s) 35, 132
HhaI GCGC 2 cut(s) 156, 292
Hin1II CATG 3 cut(s) 296, 386, 555
Hin6I GCGC 2 cut(s) 154, 290
HinP1I GCGC 2 cut(s) 154, 290
HincII GTYRAC 1 cut(s) 454
HindII GTYRAC 1 cut(s) 454
HinfI GANTC 2 cut(s) 31, 117
HpaII CCGG 2 cut(s) 35, 132
Hpy166II GTNNAC 1 cut(s) 454
Hpy188I TCNGA 1 cut(s) 116
Hpy188III TCNNGA 3 cut(s) 190, 438, 552
Hpy8I GTNNAC 1 cut(s) 454
Hpy99I CGWCG 1 cut(s) 458
HpyAV CCTTC 2 cut(s) 207, 316
HpyCH4III ACNGT 1 cut(s) 433
HpyCH4IV ACGT 2 cut(s) 207, 342
HpyCH4V TGCA 2 cut(s) 59, 95
HpyF10VI GCNNNNNNNGC 4 cut(s) 101, 283, 289, 310
HpyF3I CTNAG 2 cut(s) 78, 480
HpySE526I ACGT 2 cut(s) 207, 342
Hsp92II CATG 3 cut(s) 296, 386, 555
HspAI GCGC 2 cut(s) 154, 290
Ksp22I TGATCA 2 cut(s) 111, 145
Kzo9I GATC 4 cut(s) 111, 145, 181, 460
LguI GCTCTTC 1 cut(s) 130
LmnI GCTCC 3 cut(s) 217, 301, 489
Lsp1109I GCAGC 2 cut(s) 295, 325
MaeII ACGT 2 cut(s) 207, 342
MaeIII GTNAC 2 cut(s) 338, 343
MalI GATC 4 cut(s) 113, 147, 183, 462
MboI GATC 4 cut(s) 111, 145, 181, 460
MboII GAAGA 3 cut(s) 117, 470, 517
MflI RGATCY 1 cut(s) 460
MhlI GDGCHC 1 cut(s) 486
MspI CCGG 2 cut(s) 35, 132
MwoI GCNNNNNNNGC 4 cut(s) 101, 283, 289, 310
NdeI CATATG 1 cut(s) 150
NdeII GATC 4 cut(s) 111, 145, 181, 460
NlaIII CATG 3 cut(s) 296, 386, 555
NlaIV GGNNCC 1 cut(s) 351
NmuCI GTSAC 2 cut(s) 338, 343
NsbI TGCGCA 1 cut(s) 291
NspI RCATGY 1 cut(s) 296
PagI TCATGA 1 cut(s) 551
PciSI GCTCTTC 1 cut(s) 130
PfeI GAWTC 2 cut(s) 31, 117
PkrI GCNGC 2 cut(s) 285, 315
PshAI GACNNNNGTC 1 cut(s) 332
Psp124BI GAGCTC 1 cut(s) 486
Psp1406I AACGTT 1 cut(s) 207
PspFI CCCAGC 1 cut(s) 514
PspN4I GGNNCC 1 cut(s) 351
PsuI RGATCY 1 cut(s) 460
RsaI GTAC 1 cut(s) 142
RsaNI GTAC 1 cut(s) 141
SacI GAGCTC 1 cut(s) 486
SalI GTCGAC 1 cut(s) 452
SapI GCTCTTC 1 cut(s) 130
SatI GCNGC 2 cut(s) 284, 314
Sau3AI GATC 4 cut(s) 111, 145, 181, 460
ScaI AGTACT 1 cut(s) 142
SduI GDGCHC 1 cut(s) 486
SetI ASST 7 cut(s) 106, 125, 210, 256, 345, 411, 486
SsiI CCGC 2 cut(s) 353, 502
SstI GAGCTC 1 cut(s) 486
TaaI ACNGT 1 cut(s) 433
TaiI ACGT 2 cut(s) 210, 345
TaqI TCGA 5 cut(s) 87, 180, 220, 320, 453
TatI WGTACW 1 cut(s) 140
TfiI GAWTC 2 cut(s) 31, 117
TseFI GTSAC 2 cut(s) 338, 343
TseI GCWGC 2 cut(s) 283, 313
Tsp45I GTSAC 2 cut(s) 338, 343
TspDTI ATGAA 1 cut(s) 540
TspGWI ACGGA 1 cut(s) 362
XceI RCATGY 1 cut(s) 296
XmiI GTMKAC 1 cut(s) 453
ZrmI AGTACT 1 cut(s) 142
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.