pycom04g20920

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0004619
Physical Location & Seq
Reverse (-)
22334117 .. 22335822
1706 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g20920.3

Sequence Viewer

Length: 375 bp
ATGCGTCCCGACTTGTATGCTGTTCCTGTCGATCACTTCAAGAAGCCTTCTCGTAATGTTGCTCCTTCTCGGAAGGCAACAAGAGGAGTTGGTGGGGGAGGAGGAGGTGGAGGAGGGAGGGAAAAGAGAGGTCAGCGTGCGCATGTTATGGAGCAAAAGAGGCAGCAATCGAAGGTGGCAAAAGTCTGTGACGTGACCGAACCGCCAACTAAACCCTATCACTATCAACTCCATGTCCAACAACCCAATCGCACTCACCACCACCACCACCACCGCGACACCGTTCCAAGAAGACCTCCCAAGCCTGTCGACGAAGATCGATCTACAAAATACCCCCTGAGCTCCTCCACACTACCAAGCGGAAGAAAATGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

125

Amino Acids

13.93

Weight (kDa)

10.53

Isoelectric Point (pI)

68.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 141
AccI GTMKAC 1 cut(s) 309
AccII CGCG 1 cut(s) 276
AciI CCGC 3 cut(s) 203, 274, 360
AgsI TTSAA 1 cut(s) 40
AjiI CACGTC 1 cut(s) 193
AluBI AGCT 1 cut(s) 342
AluI AGCT 1 cut(s) 342
Alw21I GWGCWC 1 cut(s) 344
ApeKI GCWGC 1 cut(s) 163
AspLEI GCGC 1 cut(s) 142
AsuHPI GGTGA 1 cut(s) 248
BanII GRGCYC 1 cut(s) 344
BbsI GAAGAC 1 cut(s) 298
Bbv12I GWGCWC 1 cut(s) 344
BbvI GCAGC 1 cut(s) 175
BcgI CGANNNNNNTGC 1 cut(s) 33
BisI GCNGC 1 cut(s) 164
BlsI GCNGC 1 cut(s) 165
BmgBI CACGTC 1 cut(s) 193
BpiI GAAGAC 1 cut(s) 298
Bpu10I CCTNAGC 1 cut(s) 338
Bsa29I ATCGAT 1 cut(s) 319
BseCI ATCGAT 1 cut(s) 319
BseMII CTCAG 1 cut(s) 329
BseRI GAGGAG 5 cut(s) 99, 114, 117, 126, 334
BseXI GCAGC 1 cut(s) 175
Bsh1236I CGCG 1 cut(s) 276
BshVI ATCGAT 1 cut(s) 319
BsiHKAI GWGCWC 1 cut(s) 344
Bsp1286I GDGCHC 1 cut(s) 344
Bsp143I GATC 3 cut(s) 31, 316, 320
BspACI CCGC 3 cut(s) 203, 274, 360
BspCNI CTCAG 1 cut(s) 330
BspDI ATCGAT 1 cut(s) 319
BspFNI CGCG 1 cut(s) 276
BssMI GATC 3 cut(s) 31, 316, 320
Bst4CI ACNGT 1 cut(s) 283
BstC8I GCNNGC 1 cut(s) 138
BstDEI CTNAG 1 cut(s) 338
BstFNI CGCG 1 cut(s) 276
BstHHI GCGC 1 cut(s) 142
BstKTI GATC 3 cut(s) 34, 319, 323
BstMBI GATC 3 cut(s) 31, 316, 320
BstMWI GCNNNNNNNGC 1 cut(s) 160
BstNSI RCATGY 1 cut(s) 146
BstUI CGCG 1 cut(s) 276
BstV1I GCAGC 1 cut(s) 175
BstV2I GAAGAC 1 cut(s) 298
Bsu15I ATCGAT 1 cut(s) 319
BsuTUI ATCGAT 1 cut(s) 319
BtrI CACGTC 1 cut(s) 193
Cac8I GCNNGC 1 cut(s) 138
CfoI GCGC 1 cut(s) 142
ClaI ATCGAT 1 cut(s) 319
CviAII CATG 2 cut(s) 143, 233
CviJI RGCY 3 cut(s) 46, 304, 342
CviKI_1 RGCY 3 cut(s) 46, 304, 342
DdeI CTNAG 1 cut(s) 338
DpnI GATC 3 cut(s) 33, 318, 322
DpnII GATC 3 cut(s) 31, 316, 320
Ecl136II GAGCTC 1 cut(s) 342
Eco24I GRGCYC 1 cut(s) 344
Eco53kI GAGCTC 1 cut(s) 342
EcoICRI GAGCTC 1 cut(s) 342
EcoT38I GRGCYC 1 cut(s) 344
FaeI CATG 2 cut(s) 146, 236
FaiI YATR 4 cut(s) 18, 144, 149, 234
FatI CATG 2 cut(s) 142, 232
FblI GTMKAC 1 cut(s) 309
Fnu4HI GCNGC 1 cut(s) 164
FriOI GRGCYC 1 cut(s) 344
Fsp4HI GCNGC 1 cut(s) 164
FspAI RTGCGCAY 1 cut(s) 141
FspI TGCGCA 1 cut(s) 141
GlaI GCGC 1 cut(s) 141
GluI GCNGC 1 cut(s) 164
HhaI GCGC 1 cut(s) 142
Hin1II CATG 2 cut(s) 146, 236
Hin6I GCGC 1 cut(s) 140
HinP1I GCGC 1 cut(s) 140
HincII GTYRAC 1 cut(s) 310
HindII GTYRAC 1 cut(s) 310
HphI GGTGA 1 cut(s) 248
Hpy166II GTNNAC 1 cut(s) 310
Hpy188I TCNGA 1 cut(s) 72
Hpy188III TCNNGA 2 cut(s) 8, 40
Hpy8I GTNNAC 1 cut(s) 310
Hpy99I CGWCG 1 cut(s) 314
HpyAV CCTTC 4 cut(s) 57, 67, 75, 166
HpyCH4III ACNGT 1 cut(s) 283
HpyCH4IV ACGT 1 cut(s) 192
HpyF10VI GCNNNNNNNGC 1 cut(s) 160
HpyF3I CTNAG 1 cut(s) 338
HpySE526I ACGT 1 cut(s) 192
Hsp92II CATG 2 cut(s) 146, 236
HspAI GCGC 1 cut(s) 140
Kzo9I GATC 3 cut(s) 31, 316, 320
LmnI GCTCC 3 cut(s) 67, 151, 347
LpnPI CCDG 3 cut(s) 39, 318, 350
Lsp1109I GCAGC 1 cut(s) 175
MaeII ACGT 1 cut(s) 192
MaeIII GTNAC 2 cut(s) 188, 193
MalI GATC 3 cut(s) 33, 318, 322
MboI GATC 3 cut(s) 31, 316, 320
MboII GAAGA 3 cut(s) 303, 326, 375
MhlI GDGCHC 1 cut(s) 344
MmeI TCCRAC 1 cut(s) 262
MvnI CGCG 1 cut(s) 276
MwoI GCNNNNNNNGC 1 cut(s) 160
NdeII GATC 3 cut(s) 31, 316, 320
NlaIII CATG 2 cut(s) 146, 236
NmuCI GTSAC 2 cut(s) 188, 193
NsbI TGCGCA 1 cut(s) 141
NspI RCATGY 1 cut(s) 146
PkrI GCNGC 1 cut(s) 165
Psp124BI GAGCTC 1 cut(s) 344
SacI GAGCTC 1 cut(s) 344
SalI GTCGAC 1 cut(s) 308
SatI GCNGC 1 cut(s) 164
Sau3AI GATC 3 cut(s) 31, 316, 320
SduI GDGCHC 1 cut(s) 344
SetI ASST 6 cut(s) 109, 133, 177, 195, 298, 344
SsiI CCGC 3 cut(s) 203, 274, 360
SstI GAGCTC 1 cut(s) 344
TaaI ACNGT 1 cut(s) 283
TaiI ACGT 1 cut(s) 195
TaqI TCGA 4 cut(s) 30, 170, 309, 319
TaqII GACCGA 1 cut(s) 212
TseFI GTSAC 2 cut(s) 188, 193
TseI GCWGC 1 cut(s) 163
Tsp45I GTSAC 2 cut(s) 188, 193
XceI RCATGY 1 cut(s) 146
XmiI GTMKAC 1 cut(s) 309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.