Prupe.6G355600_v2.0.a1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0004619
Physical Location & Seq
Reverse (-)
30193764 .. 30196399
2636 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G355600.5

Sequence Viewer

Length: 555 bp
ATGGATCAGGACTACTATAAAAGGAGTGGTCAAATACCGGCATTTGGAGACTGGGATTATGCAAACGAGCTGCCAATCACCCAGTATTTCGAGTGCGCAAGACAGGCCGGTTTGATCAGATTCAGCTCTTCCTCTGGTGAAAGTGCTGATGCCTACGTGCGTTCTGCTGACTTGTATGCTGTTAATCATCTCAACAAGCCTAAACCCTCTCGAAAGCAGCAGGCAGCAACAACAAGAGTAGGGACGAGGGAAAAGCGAGGCCAGCGTGCGCATGTCATGGAGCAAAAGAAGCAGCAAGCTAAGGTCTGTGACGTGACGCAACCGCCAACTAAACACTACGAATATCAAGTTCAACAATCAAATCGCACTCGCTCGAACGACGCCGTTCCGAGAGTACCCCCTCGTTTCCCCACTAGGCCTCCAAAGCCCGTGGACGAAGATCTCTACAAAATCCCCCCTGAGCTTCTCCATAAGAACAAGCGGAAGAAAAGAAGAAAAGAAGATAAGAAAATGCTGGGATTGCTTTGTTGCTTGGTGCCAGCATGTGCTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

185

Amino Acids

21.16

Weight (kDa)

9.75

Isoelectric Point (pI)

70.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 97, 270
AccB1I GGYRCC 1 cut(s) 535
AciI CCGC 2 cut(s) 323, 481
AclWI GGATC 1 cut(s) 12
AcyI GRCGYC 1 cut(s) 381
AfaI GTAC 1 cut(s) 396
AfiI CCNNNNNNNGG 1 cut(s) 44
AgsI TTSAA 1 cut(s) 353
AjiI CACGTC 1 cut(s) 313
AluBI AGCT 4 cut(s) 70, 126, 299, 463
AluI AGCT 4 cut(s) 70, 126, 299, 463
Alw26I GTCTC 1 cut(s) 42
AlwI GGATC 1 cut(s) 12
AoxI GGCC 3 cut(s) 105, 259, 416
ApeKI GCWGC 4 cut(s) 70, 217, 224, 292
AspLEI GCGC 2 cut(s) 98, 271
AsuHPI GGTGA 2 cut(s) 70, 149
BanI GGYRCC 1 cut(s) 535
BbvI GCAGC 4 cut(s) 57, 229, 236, 304
BceAI ACGGC 1 cut(s) 368
BclI TGATCA 1 cut(s) 114
BcoDI GTCTC 1 cut(s) 42
BfaI CTAG 1 cut(s) 414
BglII AGATCT 1 cut(s) 439
BisI GCNGC 4 cut(s) 71, 218, 225, 293
BlsI GCNGC 4 cut(s) 72, 219, 226, 294
BmgBI CACGTC 1 cut(s) 313
BmiI GGNNCC 1 cut(s) 537
BmrI ACTGGG 2 cut(s) 61, 76
BmsI GCATC 1 cut(s) 139
BmuI ACTGGG 2 cut(s) 61, 76
Bpu10I CCTNAGC 2 cut(s) 300, 459
BsaAI YACGTR 1 cut(s) 157
BsaHI GRCGYC 1 cut(s) 381
BsaJI CCNNGG 1 cut(s) 429
BsaXI ACNNNNNCTCC 2 cut(s) 403, 433
Bsc4I CCNNNNNNNGG 1 cut(s) 44
Bse118I RCCGGY 2 cut(s) 37, 107
Bse1I ACTGG 2 cut(s) 56, 82
BseDI CCNNGG 1 cut(s) 429
BseLI CCNNNNNNNGG 1 cut(s) 44
BseMII CTCAG 1 cut(s) 450
BseNI ACTGG 2 cut(s) 56, 82
BseXI GCAGC 4 cut(s) 57, 229, 236, 304
BseYI CCCAGC 1 cut(s) 514
BshFI GGCC 3 cut(s) 107, 261, 418
BshNI GGYRCC 1 cut(s) 535
BsiSI CCGG 2 cut(s) 38, 108
BslFI GGGAC 1 cut(s) 256
BslI CCNNNNNNNGG 1 cut(s) 44
BsmAI GTCTC 1 cut(s) 42
BsmFI GGGAC 1 cut(s) 256
BsnI GGCC 3 cut(s) 107, 261, 418
Bsp143I GATC 3 cut(s) 4, 114, 439
BspACI CCGC 2 cut(s) 323, 481
BspANI GGCC 3 cut(s) 107, 261, 418
BspCNI CTCAG 1 cut(s) 451
BspHI TCATGA 1 cut(s) 551
BspLI GGNNCC 1 cut(s) 537
BspPI GGATC 1 cut(s) 12
BspQI GCTCTTC 1 cut(s) 133
BspT107I GGYRCC 1 cut(s) 535
BsrFI RCCGGY 2 cut(s) 37, 107
BsrI ACTGG 2 cut(s) 56, 82
BssAI RCCGGY 2 cut(s) 37, 107
BssECI CCNNGG 1 cut(s) 429
BssMI GATC 3 cut(s) 4, 114, 439
BssNI GRCGYC 1 cut(s) 381
Bst6I CTCTTC 1 cut(s) 133
BstACI GRCGYC 1 cut(s) 381
BstBAI YACGTR 1 cut(s) 157
BstC8I GCNNGC 5 cut(s) 222, 263, 267, 297, 540
BstDEI CTNAG 2 cut(s) 300, 459
BstDSI CCRYGG 1 cut(s) 429
BstHHI GCGC 2 cut(s) 98, 271
BstKTI GATC 3 cut(s) 7, 117, 442
BstMAI GTCTC 1 cut(s) 42
BstMBI GATC 3 cut(s) 4, 114, 439
BstMWI GCNNNNNNNGC 5 cut(s) 104, 262, 289, 424, 520
BstNSI RCATGY 2 cut(s) 275, 546
BstV1I GCAGC 4 cut(s) 57, 229, 236, 304
BstX2I RGATCY 1 cut(s) 439
BstYI RGATCY 1 cut(s) 439
BsuRI GGCC 3 cut(s) 107, 261, 418
BtgI CCRYGG 1 cut(s) 429
BtrI CACGTC 1 cut(s) 313
Cac8I GCNNGC 5 cut(s) 222, 263, 267, 297, 540
CciI TCATGA 1 cut(s) 551
CfoI GCGC 2 cut(s) 98, 271
Cfr10I RCCGGY 2 cut(s) 37, 107
CseI GACGC 2 cut(s) 325, 389
Csp6I GTAC 1 cut(s) 395
CspCI CAANNNNNGTGG 2 cut(s) 411, 446
CviAII CATG 4 cut(s) 272, 277, 543, 552
CviJI RGCY 9 cut(s) 70, 107, 126, 199, 261, 299, 418, 427, 463
CviKI_1 RGCY 9 cut(s) 70, 107, 126, 199, 261, 299, 418, 427, 463
CviQI GTAC 1 cut(s) 395
DdeI CTNAG 2 cut(s) 300, 459
DpnI GATC 3 cut(s) 6, 116, 441
DpnII GATC 3 cut(s) 4, 114, 439
Eam1104I CTCTTC 1 cut(s) 133
EarI CTCTTC 1 cut(s) 133
Eco147I AGGCCT 1 cut(s) 418
FaeI CATG 4 cut(s) 275, 280, 546, 555
FaiI YATR 8 cut(s) 18, 60, 177, 273, 278, 471, 544, 553
FaqI GGGAC 1 cut(s) 256
FatI CATG 4 cut(s) 271, 276, 542, 551
FbaI TGATCA 1 cut(s) 114
Fnu4HI GCNGC 4 cut(s) 71, 218, 225, 293
Fsp4HI GCNGC 4 cut(s) 71, 218, 225, 293
FspAI RTGCGCAY 1 cut(s) 270
FspBI CTAG 1 cut(s) 414
FspI TGCGCA 2 cut(s) 97, 270
GlaI GCGC 2 cut(s) 97, 270
GluI GCNGC 4 cut(s) 71, 218, 225, 293
GsaI CCCAGC 1 cut(s) 518
HaeIII GGCC 3 cut(s) 107, 261, 418
HapII CCGG 2 cut(s) 38, 108
HgaI GACGC 2 cut(s) 325, 389
HhaI GCGC 2 cut(s) 98, 271
Hin1I GRCGYC 1 cut(s) 381
Hin1II CATG 4 cut(s) 275, 280, 546, 555
Hin6I GCGC 2 cut(s) 96, 269
HinP1I GCGC 2 cut(s) 96, 269
HinfI GANTC 1 cut(s) 120
HpaII CCGG 2 cut(s) 38, 108
HphI GGTGA 2 cut(s) 70, 149
Hpy166II GTNNAC 1 cut(s) 433
Hpy188I TCNGA 2 cut(s) 119, 390
Hpy188III TCNNGA 3 cut(s) 8, 210, 552
Hpy8I GTNNAC 1 cut(s) 433
Hpy99I CGWCG 1 cut(s) 383
HpyCH4IV ACGT 2 cut(s) 156, 312
HpyCH4V TGCA 1 cut(s) 62
HpyF10VI GCNNNNNNNGC 5 cut(s) 104, 262, 289, 424, 520
HpyF3I CTNAG 2 cut(s) 300, 459
HpySE526I ACGT 2 cut(s) 156, 312
Hsp92I GRCGYC 1 cut(s) 381
Hsp92II CATG 4 cut(s) 275, 280, 546, 555
HspAI GCGC 2 cut(s) 96, 269
Ksp22I TGATCA 1 cut(s) 114
Kzo9I GATC 3 cut(s) 4, 114, 439
LguI GCTCTTC 1 cut(s) 133
LmnI GCTCC 1 cut(s) 280
Lsp1109I GCAGC 4 cut(s) 57, 229, 236, 304
LweI GCATC 1 cut(s) 139
MaeI CTAG 1 cut(s) 414
MaeII ACGT 2 cut(s) 156, 312
MaeIII GTNAC 2 cut(s) 308, 313
MalI GATC 3 cut(s) 6, 116, 441
MboI GATC 3 cut(s) 4, 114, 439
MboII GAAGA 5 cut(s) 120, 449, 496, 504, 512
MflI RGATCY 1 cut(s) 439
MnlI CCTC 6 cut(s) 142, 217, 240, 251, 411, 429
MseI TTAA 1 cut(s) 183
MspI CCGG 2 cut(s) 38, 108
MwoI GCNNNNNNNGC 5 cut(s) 104, 262, 289, 424, 520
NdeII GATC 3 cut(s) 4, 114, 439
NlaIII CATG 4 cut(s) 275, 280, 546, 555
NlaIV GGNNCC 1 cut(s) 537
NmuCI GTSAC 2 cut(s) 308, 313
NsbI TGCGCA 2 cut(s) 97, 270
NspI RCATGY 2 cut(s) 275, 546
PagI TCATGA 1 cut(s) 551
PceI AGGCCT 1 cut(s) 418
PciSI GCTCTTC 1 cut(s) 133
PfeI GAWTC 1 cut(s) 120
PkrI GCNGC 4 cut(s) 72, 219, 226, 294
Ppu21I YACGTR 1 cut(s) 157
PspFI CCCAGC 1 cut(s) 514
PspN4I GGNNCC 1 cut(s) 537
PsuI RGATCY 1 cut(s) 439
RsaI GTAC 1 cut(s) 396
RsaNI GTAC 1 cut(s) 395
SapI GCTCTTC 1 cut(s) 133
SaqAI TTAA 1 cut(s) 183
SatI GCNGC 4 cut(s) 71, 218, 225, 293
Sau3AI GATC 3 cut(s) 4, 114, 439
SetI ASST 7 cut(s) 72, 128, 159, 301, 306, 315, 465
SfaNI GCATC 1 cut(s) 139
SseBI AGGCCT 1 cut(s) 418
SsiI CCGC 2 cut(s) 323, 481
SspMI CTAG 1 cut(s) 414
StuI AGGCCT 1 cut(s) 418
TaiI ACGT 2 cut(s) 159, 315
TaqI TCGA 3 cut(s) 90, 211, 374
TfiI GAWTC 1 cut(s) 120
Tru1I TTAA 1 cut(s) 183
Tru9I TTAA 1 cut(s) 183
TseFI GTSAC 2 cut(s) 308, 313
TseI GCWGC 4 cut(s) 70, 217, 224, 292
Tsp45I GTSAC 2 cut(s) 308, 313
TspDTI ATGAA 1 cut(s) 540
XceI RCATGY 2 cut(s) 275, 546
XspI CTAG 1 cut(s) 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.