MD12G1253400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0004619
Physical Location & Seq
Reverse (-)
32221730 .. 32224964
3235 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1253400.v1.1.491

Sequence Viewer

Length: 564 bp
ATGGATGACTATTACAAGAGGAGTGGGCAGATTCCGGCGTTTGGGGACTGGGATTATGCAAACGAACTGCCAATCACTCAGTATTTCGAGTGTGCAAGACAAGCTGGTTTGATCAGATTCAGCTCTTCCTCCGGCGAGAGTACTGATCATATGCGCCCTGACTTGTATGCTGTTCCTGTCGATCACTTCAAGAAGCCTTCTCGCAACGTTGCTCCTCCTCCAAAGGCAACAGGAGGAGGAGGCGGAGGAGGAGGTGGTGGTGGTGGTGGGGTAGGGAGGGAAAAGCGAGGGCAGCGTGCGCATGTTATGGAGCAAAAGAGGCAGCAATCGAAGGGGACAAAAGTCTGTGACGTGACGGAACCGCCAACTAAGCCCTATCACAGTCAACTCCATGTTCAACAAACCAATCGCACTCACCTCCACCACCACAAGAACGACACCGTTTCAAGACCCAAGCCTGTCGACGAAGATCTCTACAAGATACCCCCTGAGCTCCTCCACACTACCAAGCGGAAGAAAATGCTGGGATTGTTTGCTTGCTTGGTGCCTGCTTGTGCTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

188

Amino Acids

20.62

Weight (kDa)

9.37

Isoelectric Point (pI)

65.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 300
AccB1I GGYRCC 1 cut(s) 544
AccI GTMKAC 1 cut(s) 462
AciI CCGC 3 cut(s) 243, 362, 511
AclI AACGTT 1 cut(s) 207
AfaI GTAC 1 cut(s) 142
AfiI CCNNNNNNNGG 1 cut(s) 41
AgsI TTSAA 3 cut(s) 190, 398, 447
AjiI CACGTC 1 cut(s) 352
AluBI AGCT 3 cut(s) 104, 123, 493
AluI AGCT 3 cut(s) 104, 123, 493
Alw21I GWGCWC 1 cut(s) 495
ApeKI GCWGC 2 cut(s) 292, 322
AspLEI GCGC 2 cut(s) 156, 301
AsuHPI GGTGA 1 cut(s) 407
BanI GGYRCC 1 cut(s) 544
BanII GRGCYC 1 cut(s) 495
Bbv12I GWGCWC 1 cut(s) 495
BbvI GCAGC 2 cut(s) 304, 334
BclI TGATCA 2 cut(s) 111, 145
BglII AGATCT 1 cut(s) 469
BisI GCNGC 2 cut(s) 293, 323
BlsI GCNGC 2 cut(s) 294, 324
BmcAI AGTACT 1 cut(s) 142
BmgBI CACGTC 1 cut(s) 352
BmiI GGNNCC 2 cut(s) 360, 546
BmrI ACTGGG 1 cut(s) 58
BmuI ACTGGG 1 cut(s) 58
BoxI GACNNNNGTC 1 cut(s) 341
Bpu10I CCTNAGC 1 cut(s) 489
Bsc4I CCNNNNNNNGG 1 cut(s) 41
Bse1I ACTGG 1 cut(s) 53
BseGI GGATG 1 cut(s) 10
BseLI CCNNNNNNNGG 1 cut(s) 41
BseMII CTCAG 2 cut(s) 92, 480
BseNI ACTGG 1 cut(s) 53
BseRI GAGGAG 8 cut(s) 34, 204, 207, 249, 252, 261, 264, 485
BseXI GCAGC 2 cut(s) 304, 334
BseYI CCCAGC 1 cut(s) 523
BshNI GGYRCC 1 cut(s) 544
BsiHKAI GWGCWC 1 cut(s) 495
BsiSI CCGG 2 cut(s) 35, 132
BslFI GGGAC 2 cut(s) 59, 349
BslI CCNNNNNNNGG 1 cut(s) 41
BsmFI GGGAC 2 cut(s) 59, 349
Bsp1286I GDGCHC 1 cut(s) 495
Bsp143I GATC 4 cut(s) 111, 145, 181, 469
BspACI CCGC 3 cut(s) 243, 362, 511
BspCNI CTCAG 2 cut(s) 91, 481
BspHI TCATGA 1 cut(s) 560
BspLI GGNNCC 2 cut(s) 360, 546
BspQI GCTCTTC 1 cut(s) 130
BspT107I GGYRCC 1 cut(s) 544
BsrI ACTGG 1 cut(s) 53
BssMI GATC 4 cut(s) 111, 145, 181, 469
Bst4CI ACNGT 2 cut(s) 383, 442
Bst6I CTCTTC 1 cut(s) 130
BstC8I GCNNGC 3 cut(s) 297, 538, 549
BstDEI CTNAG 3 cut(s) 78, 369, 489
BstF5I GGATG 1 cut(s) 10
BstHHI GCGC 2 cut(s) 156, 301
BstKTI GATC 4 cut(s) 114, 148, 184, 472
BstMBI GATC 4 cut(s) 111, 145, 181, 469
BstMWI GCNNNNNNNGC 5 cut(s) 101, 292, 298, 319, 370
BstNSI RCATGY 1 cut(s) 305
BstPAI GACNNNNGTC 1 cut(s) 341
BstV1I GCAGC 2 cut(s) 304, 334
BstX2I RGATCY 1 cut(s) 469
BstYI RGATCY 1 cut(s) 469
BtrI CACGTC 1 cut(s) 352
BtsCI GGATG 1 cut(s) 10
Cac8I GCNNGC 3 cut(s) 297, 538, 549
CciI TCATGA 1 cut(s) 560
CfoI GCGC 2 cut(s) 156, 301
Csp6I GTAC 1 cut(s) 141
CspCI CAANNNNNGTGG 2 cut(s) 4, 39
CviAII CATG 3 cut(s) 302, 392, 561
CviJI RGCY 6 cut(s) 104, 123, 196, 373, 457, 493
CviKI_1 RGCY 6 cut(s) 104, 123, 196, 373, 457, 493
CviQI GTAC 1 cut(s) 141
DdeI CTNAG 3 cut(s) 78, 369, 489
DpnI GATC 4 cut(s) 113, 147, 183, 471
DpnII GATC 4 cut(s) 111, 145, 181, 469
Eam1104I CTCTTC 1 cut(s) 130
EarI CTCTTC 1 cut(s) 130
EciI GGCGGA 1 cut(s) 258
Ecl136II GAGCTC 1 cut(s) 493
Eco24I GRGCYC 1 cut(s) 495
Eco53kI GAGCTC 1 cut(s) 493
EcoICRI GAGCTC 1 cut(s) 493
EcoT38I GRGCYC 1 cut(s) 495
FaeI CATG 3 cut(s) 305, 395, 564
FaiI YATR 8 cut(s) 57, 150, 152, 168, 303, 308, 393, 562
FaqI GGGAC 2 cut(s) 59, 349
FatI CATG 3 cut(s) 301, 391, 560
FauNDI CATATG 1 cut(s) 150
FbaI TGATCA 2 cut(s) 111, 145
FblI GTMKAC 1 cut(s) 462
Fnu4HI GCNGC 2 cut(s) 293, 323
FokI GGATG 1 cut(s) 17
FriOI GRGCYC 1 cut(s) 495
Fsp4HI GCNGC 2 cut(s) 293, 323
FspAI RTGCGCAY 1 cut(s) 300
FspI TGCGCA 1 cut(s) 300
GlaI GCGC 2 cut(s) 155, 300
GluI GCNGC 2 cut(s) 293, 323
GsaI CCCAGC 1 cut(s) 527
HapII CCGG 2 cut(s) 35, 132
HhaI GCGC 2 cut(s) 156, 301
Hin1II CATG 3 cut(s) 305, 395, 564
Hin6I GCGC 2 cut(s) 154, 299
HinP1I GCGC 2 cut(s) 154, 299
HincII GTYRAC 2 cut(s) 386, 463
HindII GTYRAC 2 cut(s) 386, 463
HinfI GANTC 2 cut(s) 31, 117
HpaII CCGG 2 cut(s) 35, 132
HphI GGTGA 1 cut(s) 407
Hpy166II GTNNAC 2 cut(s) 386, 463
Hpy188I TCNGA 1 cut(s) 116
Hpy188III TCNNGA 3 cut(s) 190, 447, 561
Hpy8I GTNNAC 2 cut(s) 386, 463
Hpy99I CGWCG 1 cut(s) 467
HpyAV CCTTC 2 cut(s) 207, 325
HpyCH4III ACNGT 2 cut(s) 383, 442
HpyCH4IV ACGT 2 cut(s) 207, 351
HpyCH4V TGCA 2 cut(s) 59, 95
HpyF10VI GCNNNNNNNGC 5 cut(s) 101, 292, 298, 319, 370
HpyF3I CTNAG 3 cut(s) 78, 369, 489
HpySE526I ACGT 2 cut(s) 207, 351
Hsp92II CATG 3 cut(s) 305, 395, 564
HspAI GCGC 2 cut(s) 154, 299
Ksp22I TGATCA 2 cut(s) 111, 145
Kzo9I GATC 4 cut(s) 111, 145, 181, 469
LguI GCTCTTC 1 cut(s) 130
LmnI GCTCC 3 cut(s) 217, 310, 498
Lsp1109I GCAGC 2 cut(s) 304, 334
MaeII ACGT 2 cut(s) 207, 351
MaeIII GTNAC 2 cut(s) 347, 352
MalI GATC 4 cut(s) 113, 147, 183, 471
MboI GATC 4 cut(s) 111, 145, 181, 469
MboII GAAGA 3 cut(s) 117, 479, 526
MflI RGATCY 1 cut(s) 469
MhlI GDGCHC 1 cut(s) 495
MspI CCGG 2 cut(s) 35, 132
MwoI GCNNNNNNNGC 5 cut(s) 101, 292, 298, 319, 370
NdeI CATATG 1 cut(s) 150
NdeII GATC 4 cut(s) 111, 145, 181, 469
NlaIII CATG 3 cut(s) 305, 395, 564
NlaIV GGNNCC 2 cut(s) 360, 546
NmuCI GTSAC 2 cut(s) 347, 352
NsbI TGCGCA 1 cut(s) 300
NspI RCATGY 1 cut(s) 305
PagI TCATGA 1 cut(s) 560
PciSI GCTCTTC 1 cut(s) 130
PfeI GAWTC 2 cut(s) 31, 117
PkrI GCNGC 2 cut(s) 294, 324
PshAI GACNNNNGTC 1 cut(s) 341
Psp124BI GAGCTC 1 cut(s) 495
Psp1406I AACGTT 1 cut(s) 207
PspFI CCCAGC 1 cut(s) 523
PspN4I GGNNCC 2 cut(s) 360, 546
PsuI RGATCY 1 cut(s) 469
RsaI GTAC 1 cut(s) 142
RsaNI GTAC 1 cut(s) 141
SacI GAGCTC 1 cut(s) 495
SalI GTCGAC 1 cut(s) 461
SapI GCTCTTC 1 cut(s) 130
SatI GCNGC 2 cut(s) 293, 323
Sau3AI GATC 4 cut(s) 111, 145, 181, 469
ScaI AGTACT 1 cut(s) 142
SduI GDGCHC 1 cut(s) 495
SetI ASST 7 cut(s) 106, 125, 210, 256, 354, 420, 495
SsiI CCGC 3 cut(s) 243, 362, 511
SstI GAGCTC 1 cut(s) 495
TaaI ACNGT 2 cut(s) 383, 442
TaiI ACGT 2 cut(s) 210, 354
TaqI TCGA 4 cut(s) 87, 180, 329, 462
TatI WGTACW 1 cut(s) 140
TfiI GAWTC 2 cut(s) 31, 117
TseFI GTSAC 2 cut(s) 347, 352
TseI GCWGC 2 cut(s) 292, 322
Tsp45I GTSAC 2 cut(s) 347, 352
TspDTI ATGAA 1 cut(s) 549
TspGWI ACGGA 1 cut(s) 371
XceI RCATGY 1 cut(s) 305
XmiI GTMKAC 1 cut(s) 462
ZrmI AGTACT 1 cut(s) 142
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.