FvH4_6g03280

Protein of unknown function (DUF4005)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0015645
Physical Location & Seq
Forward (+)
1819961 .. 1821816
1856 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g03280.t1

Sequence Viewer

Length: 1140 bp
ATGGGGAAGGCAAGCAAATGGATTCTGAACTTTCTGATAGGAAAGCAGGAGAAGAAGAAGGAAGCTCTTTTGACTGAGAAATGTGCGTCTTCTACCCCCAAACCAAGCCGCCCGGGGTCTCCAAAAGTTAAGCGAAGATGGAGCTTCGGAAGATCATCTCACAGGGTTTCGAAATCTGTTGATTCGATTGACACCGCTAAACTGGCATTGAAGCCTCAGCAGAATCATGCCATATGGGTGACAATACAGGCTGAGAATGCTGCTGCTACAAGGATACAAGCCGCCTTTCGTGCTCATTTGGCAAGGAAAGCTTTACATGCCTTGAAAAGTCTAGTGAAGTTACAAGCGCTCATAAGAGGTCACTTTGTGAGGAAACAAACTAATGCTACTATGAGGCGCATGCATGCTTTGTTGGCTATCCAGGTTCGAGCTCGAGTTCAGAGAATTCAGATGGCAGAGGCAGCTGCACAGGTTGATAATAAAAAGCAGTCATCCATTAAAAGAGGTTCCCCTCATGCCAATGATGTTCATAGGAAGAAAATTTATACGACTTCCAATGAATCACAAATAGTTTCGAAGAGTACAAGCGGCTACCTGAATCATTCACAAACTGATCAGACTCATCAGAGGATTGATCATAGCTACACATCATTTTCTTCCCATCGTCTGTCCATCTCCAACCGGCACAAATACAATGAAACATTGTTAATAACACCAGAAACTAGTCCCCAAATGCCTTCACCCAAACAAAAGCCAACAAGATCCTACATCCCTTTTCAACAGCATGATTCTTTAGTTGAGCCTACATATTATGACTACTCATTCATGCCTAGTTACATGGCCAACACACAATCTTCAAGGGCCAAAAAAGTCAGGTCACAAAGTGAACCTAAGCAACGACCTGATGGGAGCAACAACTATAAAAGCAAGCACACAGAACTAGTAGATTCAACAGATGAACAGTTTCTGAGACGTTCGTCTTCACAGTTCAAAACCAATGATCAGAAAAACCATGATCTGTTTGGTAAGCTCTATAGACCAAGAAGATTGTTTGAGGATAACAAGTATGATTACATTAATCCAAGTCATTCCCAGTATACTGAATCTCTAGCTTCAGAGCCCCATGTGATATTGTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4005 IQ

Protein Analysis

380

Amino Acids

43.51

Weight (kDa)

10.23

Isoelectric Point (pI)

56.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4005 PF13178 258 - 321 8.5e-07 Protein of unknown function (DUF4005)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015645)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g03280
malus_domestica MD04G1222100.v1.1 MD12G1238300.v1.1
prunus_persica Prupe.6G341200_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0451751
rosa_laevigata RLG00000025654
rosa_multiflora Rmu_sc0004924.1_g000003 Rmu_sc0009360.1_g000003
rosa_roxburghii Rroxscaffold_164G00436340 Rroxscaffold_6G00428950
rosa_rugosa Rorug02G0635600
rosa_samantha Rh3AG037400 Rh3BG038500 Rh3CG037000 Rh3DG037700
rosa_wichuraiana Rw3G002790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1097
AciI CCGC 4 cut(s) 109, 195, 282, 588
AclWI GGATC 1 cut(s) 756
AcoI YGGCCR 1 cut(s) 840
AcsI RAATTY 2 cut(s) 444, 540
AcuI CTGAAG 1 cut(s) 1098
AdeI CACNNNGTG 2 cut(s) 367, 884
AfaI GTAC 2 cut(s) 583, 1136
AfeI AGCGCT 1 cut(s) 348
AgsI TTSAA 6 cut(s) 211, 325, 779, 858, 951, 991
AhlI ACTAGT 2 cut(s) 722, 940
AjnI CCWGG 1 cut(s) 420
AluBI AGCT 8 cut(s) 65, 144, 311, 431, 464, 642, 1030, 1112
AluI AGCT 8 cut(s) 65, 144, 311, 431, 464, 642, 1030, 1112
Alw21I GWGCWC 2 cut(s) 295, 433
Alw26I GTCTC 2 cut(s) 123, 964
AlwI GGATC 1 cut(s) 756
AlwNI CAGNNNCTG 1 cut(s) 967
Ama87I CYCGRG 2 cut(s) 112, 432
Aor51HI AGCGCT 1 cut(s) 348
AoxI GGCC 2 cut(s) 840, 861
ApeKI GCWGC 4 cut(s) 260, 263, 461, 464
ApoI RAATTY 2 cut(s) 444, 540
AseI ATTAAT 1 cut(s) 1077
Asp700I GAANNNNTTC 1 cut(s) 963
AspLEI GCGC 2 cut(s) 349, 399
AspS9I GGNCC 1 cut(s) 861
AsuC2I CCSGG 2 cut(s) 113, 114
AsuHPI GGTGA 2 cut(s) 250, 732
AsuII TTCGAA 2 cut(s) 170, 575
AvaI CYCGRG 2 cut(s) 112, 432
BalI TGGCCA 1 cut(s) 842
BanII GRGCYC 2 cut(s) 433, 1122
BbsI GAAGAC 2 cut(s) 81, 972
Bbv12I GWGCWC 2 cut(s) 295, 433
BbvCI CCTCAGC 1 cut(s) 216
BbvI GCAGC 4 cut(s) 247, 250, 451, 473
BccI CCATC 5 cut(s) 132, 445, 669, 680, 899
BciT130I CCWGG 1 cut(s) 422
BciVI GTATCC 1 cut(s) 267
BclI TGATCA 3 cut(s) 613, 634, 1000
BcnI CCSGG 2 cut(s) 113, 114
BcoDI GTCTC 2 cut(s) 123, 964
BcuI ACTAGT 2 cut(s) 722, 940
BfaI CTAG 5 cut(s) 332, 723, 831, 941, 1109
BfmI CTRYAG 1 cut(s) 1033
BfoI RGCGCY 1 cut(s) 350
BfuI GTATCC 1 cut(s) 267
BisI GCNGC 7 cut(s) 109, 261, 264, 282, 462, 465, 589
BlsI GCNGC 7 cut(s) 110, 262, 265, 283, 463, 466, 590
Bme1390I CCNGG 3 cut(s) 113, 114, 422
BmeT110I CYCGRG 2 cut(s) 112, 432
BmgT120I GGNCC 1 cut(s) 861
BmiI GGNNCC 1 cut(s) 508
BmrFI CCNGG 3 cut(s) 113, 114, 422
BmrI ACTGGG 1 cut(s) 1087
BmuI ACTGGG 1 cut(s) 1087
BoxI GACNNNNGTC 1 cut(s) 976
BpiI GAAGAC 2 cut(s) 81, 972
Bpu10I CCTNAGC 2 cut(s) 216, 891
Bpu14I TTCGAA 2 cut(s) 170, 575
BpuMI CCSGG 2 cut(s) 113, 114
BsaI GGTCTC 1 cut(s) 123
BsaJI CCNNGG 2 cut(s) 112, 113
Bse118I RCCGGY 1 cut(s) 681
Bse1I ACTGG 2 cut(s) 207, 1093
BseBI CCWGG 1 cut(s) 422
BseDI CCNNGG 2 cut(s) 112, 113
BseGI GGATG 2 cut(s) 491, 768
BseMII CTCAG 4 cut(s) 66, 230, 243, 959
BseNI ACTGG 2 cut(s) 207, 1093
BseXI GCAGC 4 cut(s) 247, 250, 451, 473
BsgI GTGCAG 1 cut(s) 450
BshFI GGCC 2 cut(s) 842, 863
BsiHKAI GWGCWC 2 cut(s) 295, 433
BsiHKCI CYCGRG 2 cut(s) 112, 432
BsiSI CCGG 2 cut(s) 113, 682
BslFI GGGAC 1 cut(s) 711
BsmAI GTCTC 2 cut(s) 123, 964
BsmBI CGTCTC 1 cut(s) 964
BsmFI GGGAC 1 cut(s) 711
BsmI GAATGC 1 cut(s) 262
BsnI GGCC 2 cut(s) 842, 863
Bso31I GGTCTC 1 cut(s) 123
BsoBI CYCGRG 2 cut(s) 112, 432
Bsp119I TTCGAA 2 cut(s) 170, 575
Bsp1286I GDGCHC 3 cut(s) 295, 433, 1122
Bsp143I GATC 6 cut(s) 152, 613, 634, 761, 1000, 1015
BspACI CCGC 4 cut(s) 109, 195, 282, 588
BspANI GGCC 2 cut(s) 842, 863
BspCNI CTCAG 4 cut(s) 67, 229, 244, 960
BspLI GGNNCC 1 cut(s) 508
BspPI GGATC 1 cut(s) 756
BspT104I TTCGAA 2 cut(s) 170, 575
BspTNI GGTCTC 1 cut(s) 123
BsrFI RCCGGY 1 cut(s) 681
BsrI ACTGG 2 cut(s) 207, 1093
BssAI RCCGGY 1 cut(s) 681
BssECI CCNNGG 2 cut(s) 112, 113
BssMI GATC 6 cut(s) 152, 613, 634, 761, 1000, 1015
BssNAI GTATAC 1 cut(s) 1098
Bst1107I GTATAC 1 cut(s) 1098
Bst2UI CCWGG 1 cut(s) 422
Bst4CI ACNGT 2 cut(s) 963, 987
Bst6I CTCTTC 1 cut(s) 572
BstBI TTCGAA 2 cut(s) 170, 575
BstC8I GCNNGC 4 cut(s) 13, 401, 405, 929
BstDEI CTNAG 5 cut(s) 75, 216, 252, 891, 968
BstF5I GGATG 2 cut(s) 491, 768
BstH2I RGCGCY 1 cut(s) 350
BstHHI GCGC 2 cut(s) 349, 399
BstKTI GATC 6 cut(s) 155, 616, 637, 764, 1003, 1018
BstMAI GTCTC 2 cut(s) 123, 964
BstMBI GATC 6 cut(s) 152, 613, 634, 761, 1000, 1015
BstMWI GCNNNNNNNGC 8 cut(s) 203, 257, 290, 299, 308, 317, 413, 461
BstNI CCWGG 1 cut(s) 422
BstNSI RCATGY 3 cut(s) 320, 403, 407
BstPAI GACNNNNGTC 1 cut(s) 976
BstSCI CCNGG 3 cut(s) 111, 112, 420
BstSFI CTRYAG 1 cut(s) 1033
BstV1I GCAGC 4 cut(s) 247, 250, 451, 473
BstV2I GAAGAC 2 cut(s) 81, 972
BstX2I RGATCY 1 cut(s) 761
BstYI RGATCY 1 cut(s) 761
BstZ17I GTATAC 1 cut(s) 1098
BsuI GTATCC 1 cut(s) 267
BsuRI GGCC 2 cut(s) 842, 863
BtsCI GGATG 2 cut(s) 491, 768
Cac8I GCNNGC 4 cut(s) 13, 401, 405, 929
CaiI CAGNNNCTG 1 cut(s) 967
CfoI GCGC 2 cut(s) 349, 399
Cfr10I RCCGGY 1 cut(s) 681
Cfr13I GGNCC 1 cut(s) 861
Cfr9I CCCGGG 1 cut(s) 112
CseI GACGC 1 cut(s) 75
Csp6I GTAC 2 cut(s) 582, 1135
CviQI GTAC 2 cut(s) 582, 1135
DdeI CTNAG 5 cut(s) 75, 216, 252, 891, 968
DpnI GATC 6 cut(s) 154, 615, 636, 763, 1002, 1017
DpnII GATC 6 cut(s) 152, 613, 634, 761, 1000, 1015
DraIII CACNNNGTG 2 cut(s) 367, 884
EaeI YGGCCR 1 cut(s) 840
Eam1104I CTCTTC 1 cut(s) 572
EarI CTCTTC 1 cut(s) 572
Ecl136II GAGCTC 1 cut(s) 431
Eco24I GRGCYC 2 cut(s) 433, 1122
Eco31I GGTCTC 1 cut(s) 123
Eco47III AGCGCT 1 cut(s) 348
Eco53kI GAGCTC 1 cut(s) 431
Eco57I CTGAAG 1 cut(s) 1098
Eco88I CYCGRG 2 cut(s) 112, 432
EcoICRI GAGCTC 1 cut(s) 431
EcoRI GAATTC 1 cut(s) 444
EcoRII CCWGG 1 cut(s) 420
EcoT22I ATGCAT 1 cut(s) 405
EcoT38I GRGCYC 2 cut(s) 433, 1122
Esp3I CGTCTC 1 cut(s) 964
FalI AAGNNNNNCTT 2 cut(s) 295, 327
FaqI GGGAC 1 cut(s) 711
FauNDI CATATG 1 cut(s) 233
FbaI TGATCA 3 cut(s) 613, 634, 1000
FblI GTMKAC 1 cut(s) 1097
Fnu4HI GCNGC 7 cut(s) 109, 261, 264, 282, 462, 465, 589
FokI GGATG 2 cut(s) 478, 755
FriOI GRGCYC 2 cut(s) 433, 1122
Fsp4HI GCNGC 7 cut(s) 109, 261, 264, 282, 462, 465, 589
FspBI CTAG 5 cut(s) 332, 723, 831, 941, 1109
GlaI GCGC 2 cut(s) 348, 398
GluI GCNGC 7 cut(s) 109, 261, 264, 282, 462, 465, 589
HaeII RGCGCY 1 cut(s) 350
HaeIII GGCC 2 cut(s) 842, 863
HapII CCGG 2 cut(s) 113, 682
HgaI GACGC 1 cut(s) 75
HhaI GCGC 2 cut(s) 349, 399
Hin6I GCGC 2 cut(s) 347, 397
HinP1I GCGC 2 cut(s) 347, 397
HindIII AAGCTT 1 cut(s) 309
HinfI GANTC 9 cut(s) 22, 182, 223, 560, 598, 619, 788, 947, 1103
HpaII CCGG 2 cut(s) 113, 682
HphI GGTGA 2 cut(s) 250, 732
Hpy166II GTNNAC 2 cut(s) 887, 1098
Hpy8I GTNNAC 2 cut(s) 887, 1098
HpyAV CCTTC 2 cut(s) 52, 747
HpyCH4III ACNGT 2 cut(s) 963, 987
HpyCH4IV ACGT 1 cut(s) 973
HpyCH4V TGCA 2 cut(s) 403, 467
HpyF10VI GCNNNNNNNGC 8 cut(s) 203, 257, 290, 299, 308, 317, 413, 461
HpyF3I CTNAG 5 cut(s) 75, 216, 252, 891, 968
HpySE526I ACGT 1 cut(s) 973
HspAI GCGC 2 cut(s) 347, 397
Ksp22I TGATCA 3 cut(s) 613, 634, 1000
Kzo9I GATC 6 cut(s) 152, 613, 634, 761, 1000, 1015
LmnI GCTCC 2 cut(s) 141, 909
Lsp1109I GCAGC 4 cut(s) 247, 250, 451, 473
MaeI CTAG 5 cut(s) 332, 723, 831, 941, 1109
MaeII ACGT 1 cut(s) 973
MaeIII GTNAC 5 cut(s) 238, 339, 359, 833, 876
MalI GATC 6 cut(s) 154, 615, 636, 763, 1002, 1017
MboI GATC 6 cut(s) 152, 613, 634, 761, 1000, 1015
MflI RGATCY 1 cut(s) 761
MhlI GDGCHC 3 cut(s) 295, 433, 1122
MlsI TGGCCA 1 cut(s) 842
MluCI AATT 2 cut(s) 444, 540
MluNI TGGCCA 1 cut(s) 842
MlyI GAGTC 1 cut(s) 613
MmeI TCCRAC 1 cut(s) 702
MnlI CCTC 9 cut(s) 225, 350, 363, 387, 451, 497, 522, 621, 1048
Mox20I TGGCCA 1 cut(s) 842
Mph1103I ATGCAT 1 cut(s) 405
MroXI GAANNNNTTC 1 cut(s) 963
MscI TGGCCA 1 cut(s) 842
MseI TTAA 4 cut(s) 129, 498, 707, 1077
MslI CAYNNNNRTG 2 cut(s) 236, 519
Msp20I TGGCCA 1 cut(s) 842
MspA1I CMGCKG 1 cut(s) 464
MspI CCGG 2 cut(s) 113, 682
MspR9I CCNGG 3 cut(s) 113, 114, 422
Mva1269I GAATGC 1 cut(s) 262
MvaI CCWGG 1 cut(s) 422
MwoI GCNNNNNNNGC 8 cut(s) 203, 257, 290, 299, 308, 317, 413, 461
NciI CCSGG 2 cut(s) 113, 114
NdeI CATATG 1 cut(s) 233
NdeII GATC 6 cut(s) 152, 613, 634, 761, 1000, 1015
NlaIV GGNNCC 1 cut(s) 508
NmuCI GTSAC 3 cut(s) 238, 359, 876
NsiI ATGCAT 1 cut(s) 405
NspI RCATGY 3 cut(s) 320, 403, 407
NspV TTCGAA 2 cut(s) 170, 575
PaeI GCATGC 2 cut(s) 403, 407
PaeR7I CTCGAG 1 cut(s) 432
PctI GAATGC 1 cut(s) 262
PdmI GAANNNNTTC 1 cut(s) 963
PfeI GAWTC 8 cut(s) 22, 182, 223, 560, 598, 788, 947, 1103
PkrI GCNGC 7 cut(s) 110, 262, 265, 283, 463, 466, 590
PleI GAGTC 1 cut(s) 613
PpsI GAGTC 1 cut(s) 613
PshAI GACNNNNGTC 1 cut(s) 976
PshBI ATTAAT 1 cut(s) 1077
Psp124BI GAGCTC 1 cut(s) 433
Psp6I CCWGG 1 cut(s) 420
PspGI CCWGG 1 cut(s) 420
PspN4I GGNNCC 1 cut(s) 508
PspPI GGNCC 1 cut(s) 861
PspXI VCTCGAGB 1 cut(s) 432
PstNI CAGNNNCTG 1 cut(s) 967
PsuI RGATCY 1 cut(s) 761
PvuII CAGCTG 1 cut(s) 464
RsaI GTAC 2 cut(s) 583, 1136
RsaNI GTAC 2 cut(s) 582, 1135
RseI CAYNNNNRTG 2 cut(s) 236, 519
SacI GAGCTC 1 cut(s) 433
SaqAI TTAA 4 cut(s) 129, 498, 707, 1077
SatI GCNGC 7 cut(s) 109, 261, 264, 282, 462, 465, 589
Sau3AI GATC 6 cut(s) 152, 613, 634, 761, 1000, 1015
Sau96I GGNCC 1 cut(s) 861
SchI GAGTC 1 cut(s) 613
ScrFI CCNGG 3 cut(s) 113, 114, 422
SduI GDGCHC 3 cut(s) 295, 433, 1122
SfcI CTRYAG 1 cut(s) 1033
Sfr274I CTCGAG 1 cut(s) 432
SfuI TTCGAA 2 cut(s) 170, 575
SlaI CTCGAG 1 cut(s) 432
SmaI CCCGGG 1 cut(s) 114
SmiMI CAYNNNNRTG 2 cut(s) 236, 519
SmlI CTYRAG 1 cut(s) 432
SmoI CTYRAG 1 cut(s) 432
SpeI ACTAGT 2 cut(s) 722, 940
SphI GCATGC 2 cut(s) 403, 407
Sse9I AATT 2 cut(s) 444, 540
SsiI CCGC 4 cut(s) 109, 195, 282, 588
SspMI CTAG 5 cut(s) 332, 723, 831, 941, 1109
SstI GAGCTC 1 cut(s) 433
StyD4I CCNGG 3 cut(s) 111, 112, 420
TaaI ACNGT 2 cut(s) 963, 987
TaiI ACGT 1 cut(s) 976
TaqI TCGA 5 cut(s) 170, 185, 427, 433, 575
TasI AATT 2 cut(s) 444, 540
TatI WGTACW 2 cut(s) 581, 1134
TauI GCSGC 3 cut(s) 111, 284, 591
TfiI GAWTC 8 cut(s) 22, 182, 223, 560, 598, 788, 947, 1103
Tru1I TTAA 4 cut(s) 129, 498, 707, 1077
Tru9I TTAA 4 cut(s) 129, 498, 707, 1077
TseFI GTSAC 3 cut(s) 238, 359, 876
TseI GCWGC 4 cut(s) 260, 263, 461, 464
Tsp45I GTSAC 3 cut(s) 238, 359, 876
TspDTI ATGAA 5 cut(s) 518, 573, 711, 814, 972
TspMI CCCGGG 1 cut(s) 112
VspI ATTAAT 1 cut(s) 1077
XapI RAATTY 2 cut(s) 444, 540
XceI RCATGY 3 cut(s) 320, 403, 407
XcmI CCANNNNNNNNNTGG 1 cut(s) 1019
XhoI CTCGAG 1 cut(s) 432
XmaI CCCGGG 1 cut(s) 112
XmiI GTMKAC 1 cut(s) 1097
XmnI GAANNNNTTC 1 cut(s) 963
XspI CTAG 5 cut(s) 332, 723, 831, 941, 1109
Zsp2I ATGCAT 1 cut(s) 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.