MD12G1238300.v1.1

Protein of unknown function (DUF4005)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0015645
Physical Location & Seq
Reverse (-)
31158685 .. 31159553
869 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1238300.v1.1.491

Sequence Viewer

Length: 630 bp
ATGGGAAAGGCAAGCAGATGGATCATCAATTTCTTGGTCGGAAAGCAAGAGAAAAAGATTAACGATGTTCCGAAAACTAACGTTTCGAATGAGTTTGCGCTTACCTTTTCTGCCAGGGCGGCGGTGAAGCAGCTAGGTACGCCGAAAGTGAAGAGAAGGTGGAGCTTTGGGATGTTGGCAGGCAAAGAGGTATCCCGCATGGTTACAAAATCTGTTGATTCAATTGACATCACTAAGTTGTCACTCAAGGCTCAGAAGAATCGTGCTGTGACACCAGGAGATGTCGAAAACGCTGCAGCTACAAGGATTCAAGCTGCCTTCCGTTCGCATTTGGCAAGGAAGGCTTTACATGCCTTGAAGGGTTTGGTGAAGTTGCAAGCACTGATAAGAGGCCACATTGTGAGGAAACAAACAACTGCTACGTTAATTCAGATGCAGGCGTTGATGGCTATCCAGGTTAGAGCGCGGATTAAGCGAATTCAGATGGCGGAAGAAGCACAGCTAGCTAATAAAAAGCAGTTGATCATTCAAAGAGGCCACCCTCAGGACAATGGAGTTCATAGGGCAAGTCTCCTCCAATCTCAAATGTTTCCTGTTCGTGCTATATGTGCTAGTTTTTGCTTTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4005 IQ

Protein Analysis

210

Amino Acids

23.28

Weight (kDa)

11.32

Isoelectric Point (pI)

38.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015645)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g03280
malus_domestica MD04G1222100.v1.1 MD12G1238300.v1.1
prunus_persica Prupe.6G341200_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0451751
rosa_laevigata RLG00000025654
rosa_multiflora Rmu_sc0004924.1_g000003 Rmu_sc0009360.1_g000003
rosa_roxburghii Rroxscaffold_164G00436340 Rroxscaffold_6G00428950
rosa_rugosa Rorug02G0635600
rosa_samantha Rh3AG037400 Rh3BG038500 Rh3CG037000 Rh3DG037700
rosa_wichuraiana Rw3G002790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 466
AciI CCGC 5 cut(s) 119, 122, 196, 466, 488
AclI AACGTT 1 cut(s) 81
AclWI GGATC 1 cut(s) 29
AcsI RAATTY 1 cut(s) 477
AdeI CACNNNGTG 1 cut(s) 400
AfaI GTAC 1 cut(s) 139
AfiI CCNNNNNNNGG 1 cut(s) 544
AgsI TTSAA 4 cut(s) 222, 311, 358, 530
AjnI CCWGG 3 cut(s) 113, 274, 453
AluBI AGCT 6 cut(s) 133, 165, 299, 314, 502, 506
AluI AGCT 6 cut(s) 133, 165, 299, 314, 502, 506
Alw26I GTCTC 1 cut(s) 575
AlwI GGATC 1 cut(s) 29
AoxI GGCC 2 cut(s) 391, 535
ApeKI GCWGC 4 cut(s) 130, 293, 296, 314
ApoI RAATTY 1 cut(s) 477
AspLEI GCGC 2 cut(s) 100, 466
AsuHPI GGTGA 2 cut(s) 136, 379
AsuII TTCGAA 1 cut(s) 86
AsuNHI GCTAGC 1 cut(s) 502
AxyI CCTNAGG 1 cut(s) 543
BbvI GCAGC 4 cut(s) 142, 280, 301, 308
BccI CCATC 3 cut(s) 12, 439, 478
BcgI CGANNNNNNTGC 2 cut(s) 275, 309
BciT130I CCWGG 3 cut(s) 115, 276, 455
BciVI GTATCC 1 cut(s) 202
BclI TGATCA 1 cut(s) 522
BcoDI GTCTC 1 cut(s) 575
BfaI CTAG 3 cut(s) 134, 503, 612
BfmI CTRYAG 1 cut(s) 294
BfuI GTATCC 1 cut(s) 202
BglI GCCNNNNNGGC 1 cut(s) 119
BisI GCNGC 5 cut(s) 120, 131, 294, 297, 315
BlsI GCNGC 5 cut(s) 121, 132, 295, 298, 316
Bme1390I CCNGG 3 cut(s) 115, 276, 455
BmrFI CCNGG 3 cut(s) 115, 276, 455
BmsI GCATC 1 cut(s) 423
BmtI GCTAGC 1 cut(s) 506
Bpu14I TTCGAA 1 cut(s) 86
BpuEI CTTGAG 1 cut(s) 230
BsaBI GATNNNNATC 1 cut(s) 449
BsaJI CCNNGG 1 cut(s) 114
Bsc4I CCNNNNNNNGG 1 cut(s) 544
Bse21I CCTNAGG 1 cut(s) 543
Bse8I GATNNNNATC 1 cut(s) 449
BseBI CCWGG 3 cut(s) 115, 276, 455
BseDI CCNNGG 1 cut(s) 114
BseGI GGATG 1 cut(s) 177
BseJI GATNNNNATC 1 cut(s) 449
BseLI CCNNNNNNNGG 1 cut(s) 544
BseMII CTCAG 2 cut(s) 266, 557
BseRI GAGGAG 1 cut(s) 563
BseXI GCAGC 4 cut(s) 142, 280, 301, 308
Bsh1236I CGCG 1 cut(s) 466
BshFI GGCC 2 cut(s) 393, 537
BslI CCNNNNNNNGG 1 cut(s) 544
BsmAI GTCTC 1 cut(s) 575
BsnI GGCC 2 cut(s) 393, 537
Bsp119I TTCGAA 1 cut(s) 86
Bsp143I GATC 2 cut(s) 21, 522
BspACI CCGC 5 cut(s) 119, 122, 196, 466, 488
BspANI GGCC 2 cut(s) 393, 537
BspCNI CTCAG 2 cut(s) 265, 556
BspFNI CGCG 1 cut(s) 466
BspMAI CTGCAG 1 cut(s) 298
BspOI GCTAGC 1 cut(s) 506
BspPI GGATC 1 cut(s) 29
BspT104I TTCGAA 1 cut(s) 86
BssECI CCNNGG 1 cut(s) 114
BssMI GATC 2 cut(s) 21, 522
Bst2UI CCWGG 3 cut(s) 115, 276, 455
Bst6I CTCTTC 1 cut(s) 146
BstBI TTCGAA 1 cut(s) 86
BstC8I GCNNGC 5 cut(s) 13, 181, 378, 438, 504
BstDEI CTNAG 3 cut(s) 234, 252, 543
BstF5I GGATG 1 cut(s) 177
BstFNI CGCG 1 cut(s) 466
BstHHI GCGC 2 cut(s) 100, 466
BstKTI GATC 2 cut(s) 24, 525
BstMAI GTCTC 1 cut(s) 575
BstMBI GATC 2 cut(s) 21, 522
BstMWI GCNNNNNNNGC 9 cut(s) 119, 139, 341, 350, 446, 472, 494, 503, 608
BstNI CCWGG 3 cut(s) 115, 276, 455
BstNSI RCATGY 1 cut(s) 353
BstSCI CCNGG 3 cut(s) 113, 274, 453
BstSFI CTRYAG 1 cut(s) 294
BstUI CGCG 1 cut(s) 466
BstV1I GCAGC 4 cut(s) 142, 280, 301, 308
Bsu36I CCTNAGG 1 cut(s) 543
BsuI GTATCC 1 cut(s) 202
BsuRI GGCC 2 cut(s) 393, 537
BtsCI GGATG 1 cut(s) 177
BtsIMutI CAGTG 1 cut(s) 380
Cac8I GCNNGC 5 cut(s) 13, 181, 378, 438, 504
CfoI GCGC 2 cut(s) 100, 466
Csp6I GTAC 1 cut(s) 138
CviAII CATG 2 cut(s) 199, 350
CviQI GTAC 1 cut(s) 138
DdeI CTNAG 3 cut(s) 234, 252, 543
DpnI GATC 2 cut(s) 23, 524
DpnII GATC 2 cut(s) 21, 522
DraIII CACNNNGTG 1 cut(s) 400
Eam1104I CTCTTC 1 cut(s) 146
EarI CTCTTC 1 cut(s) 146
EciI GGCGGA 1 cut(s) 503
Eco81I CCTNAGG 1 cut(s) 543
EcoRI GAATTC 1 cut(s) 477
EcoRII CCWGG 3 cut(s) 113, 274, 453
FaeI CATG 2 cut(s) 202, 353
FaiI YATR 5 cut(s) 200, 351, 561, 605, 607
FalI AAGNNNNNCTT 2 cut(s) 328, 360
FatI CATG 2 cut(s) 198, 349
FauI CCCGC 1 cut(s) 203
FbaI TGATCA 1 cut(s) 522
Fnu4HI GCNGC 5 cut(s) 120, 131, 294, 297, 315
FokI GGATG 1 cut(s) 184
Fsp4HI GCNGC 5 cut(s) 120, 131, 294, 297, 315
FspBI CTAG 3 cut(s) 134, 503, 612
GlaI GCGC 2 cut(s) 99, 465
GluI GCNGC 5 cut(s) 120, 131, 294, 297, 315
HaeIII GGCC 2 cut(s) 393, 537
HhaI GCGC 2 cut(s) 100, 466
Hin1II CATG 2 cut(s) 202, 353
Hin6I GCGC 2 cut(s) 98, 464
HinP1I GCGC 2 cut(s) 98, 464
HinfI GANTC 3 cut(s) 218, 259, 307
HphI GGTGA 2 cut(s) 136, 379
Hpy188I TCNGA 5 cut(s) 41, 72, 255, 432, 483
Hpy188III TCNNGA 1 cut(s) 545
HpyAV CCTTC 4 cut(s) 150, 328, 334, 352
HpyCH4IV ACGT 2 cut(s) 81, 422
HpyCH4V TGCA 3 cut(s) 296, 376, 436
HpyF10VI GCNNNNNNNGC 9 cut(s) 119, 139, 341, 350, 446, 472, 494, 503, 608
HpyF3I CTNAG 3 cut(s) 234, 252, 543
HpySE526I ACGT 2 cut(s) 81, 422
Hsp92II CATG 2 cut(s) 202, 353
HspAI GCGC 2 cut(s) 98, 464
Ksp22I TGATCA 1 cut(s) 522
Kzo9I GATC 2 cut(s) 21, 522
LmnI GCTCC 1 cut(s) 162
Lsp1109I GCAGC 4 cut(s) 142, 280, 301, 308
LweI GCATC 1 cut(s) 423
MaeI CTAG 3 cut(s) 134, 503, 612
MaeII ACGT 2 cut(s) 81, 422
MaeIII GTNAC 3 cut(s) 202, 240, 268
MalI GATC 2 cut(s) 23, 524
MboI GATC 2 cut(s) 21, 522
MboII GAAGA 3 cut(s) 163, 268, 503
MfeI CAATTG 1 cut(s) 222
MluCI AATT 4 cut(s) 28, 222, 426, 477
MmeI TCCRAC 1 cut(s) 19
MnlI CCTC 6 cut(s) 181, 383, 396, 527, 552, 584
MseI TTAA 4 cut(s) 60, 425, 471, 628
MspR9I CCNGG 3 cut(s) 115, 276, 455
MunI CAATTG 1 cut(s) 222
MvaI CCWGG 3 cut(s) 115, 276, 455
MvnI CGCG 1 cut(s) 466
MwoI GCNNNNNNNGC 9 cut(s) 119, 139, 341, 350, 446, 472, 494, 503, 608
NdeII GATC 2 cut(s) 21, 522
NheI GCTAGC 1 cut(s) 502
NlaIII CATG 2 cut(s) 202, 353
NmuCI GTSAC 2 cut(s) 240, 268
NspI RCATGY 1 cut(s) 353
NspV TTCGAA 1 cut(s) 86
PfeI GAWTC 3 cut(s) 218, 259, 307
PkrI GCNGC 5 cut(s) 121, 132, 295, 298, 316
Psp1406I AACGTT 1 cut(s) 81
Psp6I CCWGG 3 cut(s) 113, 274, 453
PspGI CCWGG 3 cut(s) 113, 274, 453
PstI CTGCAG 1 cut(s) 298
RsaI GTAC 1 cut(s) 139
RsaNI GTAC 1 cut(s) 138
SaqAI TTAA 4 cut(s) 60, 425, 471, 628
SatI GCNGC 5 cut(s) 120, 131, 294, 297, 315
Sau3AI GATC 2 cut(s) 21, 522
ScrFI CCNGG 3 cut(s) 115, 276, 455
SfaNI GCATC 1 cut(s) 423
SfcI CTRYAG 1 cut(s) 294
SfuI TTCGAA 1 cut(s) 86
SmlI CTYRAG 1 cut(s) 245
SmoI CTYRAG 1 cut(s) 245
Sse9I AATT 4 cut(s) 28, 222, 426, 477
SsiI CCGC 5 cut(s) 119, 122, 196, 466, 488
SspMI CTAG 3 cut(s) 134, 503, 612
StyD4I CCNGG 3 cut(s) 113, 274, 453
TaiI ACGT 2 cut(s) 84, 425
TaqI TCGA 2 cut(s) 86, 285
TasI AATT 4 cut(s) 28, 222, 426, 477
TauI GCSGC 1 cut(s) 122
TfiI GAWTC 3 cut(s) 218, 259, 307
Tru1I TTAA 4 cut(s) 60, 425, 471, 628
Tru9I TTAA 4 cut(s) 60, 425, 471, 628
TscAI CASTG 1 cut(s) 387
TseFI GTSAC 2 cut(s) 240, 268
TseI GCWGC 4 cut(s) 130, 293, 296, 314
Tsp45I GTSAC 2 cut(s) 240, 268
TspDTI ATGAA 1 cut(s) 548
TspGWI ACGGA 1 cut(s) 311
TspRI CASTG 1 cut(s) 387
XapI RAATTY 1 cut(s) 477
XceI RCATGY 1 cut(s) 353
XspI CTAG 3 cut(s) 134, 503, 612
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.