Rroxscaffold_6G00428950

Protein of unknown function (DUF4005)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0015645
Physical Location & Seq
Forward (+)
49327358 .. 49331064
3707 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00428950.1

Sequence Viewer

Length: 1161 bp
ATGGGGAAGGCAAGCAAATGGATTCTGAACTTTCTGATAGGAAAGCAGGAGAAGAAGAAGAATGAAGCTCTTTTGACTGAGAAATGTGTTCCCAAAGTTAAGCGAAGATGGAGCTTTGGAAGATCATCCCACAGGGTTTCAAAATCTGTTGATTCGATTGACACCACCAAATTGCCATTGAAGCCTCAGCTGAATCATGCCGTCTGGGTATCAACACAGGCTGAGAATGCTGCTGCTACAAGGATCCAAGCAGCCTTTCGTGCTCATTGGAAGGCTTTACATGCCCTGAAGAGTCTAGTGAAGTTACAAGCACTCATAAGAGGTCACTTTGTGAGGAAACAAACTAATGCTACTATGAGACGCATGCATGCTTTGTTGGCAATCCAGGTTCGAGCTCGCTGTCAGAGAATTCAGATGGCCGAGGCAGCTATACAGGAAAATAAAAAGCAGTCATCCATTAGAAGAGGTTCCCCTCATGCCAATGAAGTTCATAGGAAGAAAATCTATATGATTTCGAATGAATCACGAAGAGTTTTGAAAAGTACAAGTGACTACCTGAATCATTCACAAACTGATCAGAGGATTGATCATAGCTACACATCATTTTCTTCTGATCGTCTGTCCATCTCTAACCGACACAAATACAATGAAACCTTGTTCAGAACACCAGAAACTAGTCCCCGAATGCCTTCACCCAAACCAAAACCAACTAGGCCTTACATCCCTTTTCAACAGCATGATTCTCTAGTCGAGCCTACATGTTATGACTACTCATTCATGCCTAGTTACATGGCCAACACAGAATCTTCGACGGCCAAAAAAGTCAGGTCACAGAGTGAACCTAAGCAACGACCTGATGGGAGCAACAAGCATAAAAGCAAGCATACAGAACTAGTAGATTCAACAGATGAACAGTTTCTGAGACGTTCATCTTCACAGTTCAAAACCAATGATCACAAAAACCATGATCCCTGGTTTGGTAAGCTCTACAGACCAAGAAGATTGTTTGAGGACAACAAGTATGATTACATTAATCCAAGTCATTCCAAGTATACTGAATCTCTAGCTGCAGAGGTAAAGTGTTCAAGGATCTCCAAACAATATTTTGTGCACAGAAAGTTTTCCACCAACTTTGTTATTCAAACAAGAATCGCGTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4005 IQ

Protein Analysis

386

Amino Acids

44.97

Weight (kDa)

10.25

Isoelectric Point (pI)

56.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4005 PF13178 239 - 301 5.4e-08 Protein of unknown function (DUF4005)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015645)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g03280
malus_domestica MD04G1222100.v1.1 MD12G1238300.v1.1
prunus_persica Prupe.6G341200_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0451751
rosa_laevigata RLG00000025654
rosa_multiflora Rmu_sc0004924.1_g000003 Rmu_sc0009360.1_g000003
rosa_roxburghii Rroxscaffold_164G00436340 Rroxscaffold_6G00428950
rosa_rugosa Rorug02G0635600
rosa_samantha Rh3AG037400 Rh3BG038500 Rh3CG037000 Rh3DG037700
rosa_wichuraiana Rw3G002790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1052
AccII CGCG 1 cut(s) 1154
AclWI GGATC 4 cut(s) 238, 251, 962, 1097
AcoI YGGCCR 3 cut(s) 417, 792, 813
AcsI RAATTY 1 cut(s) 408
AcuI CTGAAG 1 cut(s) 308
AdeI CACNNNGTG 2 cut(s) 331, 836
AfaI GTAC 1 cut(s) 544
AfiI CCNNNNNNNGG 1 cut(s) 977
AflIII ACRYGT 1 cut(s) 758
AgsI TTSAA 8 cut(s) 141, 181, 538, 731, 903, 943, 1086, 1142
AhlI ACTAGT 2 cut(s) 674, 892
AjnI CCWGG 2 cut(s) 384, 971
AjuI GAANNNNNNNTTGG 2 cut(s) 240, 272
AluBI AGCT 8 cut(s) 68, 114, 190, 395, 428, 594, 985, 1067
AluI AGCT 8 cut(s) 68, 114, 190, 395, 428, 594, 985, 1067
Alw21I GWGCWC 3 cut(s) 265, 397, 1113
Alw26I GTCTC 2 cut(s) 352, 916
Alw44I GTGCAC 1 cut(s) 1109
AlwI GGATC 4 cut(s) 238, 251, 962, 1097
AlwNI CAGNNNCTG 1 cut(s) 919
AoxI GGCC 4 cut(s) 417, 713, 792, 813
ApaLI GTGCAC 1 cut(s) 1109
ApeKI GCWGC 5 cut(s) 230, 233, 251, 425, 1067
ApoI RAATTY 1 cut(s) 408
AseI ATTAAT 1 cut(s) 1032
Asp700I GAANNNNTTC 4 cut(s) 466, 688, 915, 1120
AsuHPI GGTGA 1 cut(s) 684
AsuII TTCGAA 1 cut(s) 515
BaeGI GKGCMC 1 cut(s) 1113
BalI TGGCCA 1 cut(s) 794
BamHI GGATCC 1 cut(s) 243
BanII GRGCYC 1 cut(s) 397
Bbv12I GWGCWC 3 cut(s) 265, 397, 1113
BbvCI CCTCAGC 1 cut(s) 186
BbvI GCAGC 5 cut(s) 217, 220, 263, 437, 1054
BccI CCATC 4 cut(s) 102, 409, 632, 851
BceAI ACGGC 2 cut(s) 185, 828
BciT130I CCWGG 2 cut(s) 386, 973
BclI TGATCA 3 cut(s) 574, 586, 952
BcoDI GTCTC 2 cut(s) 352, 916
BcuI ACTAGT 2 cut(s) 674, 892
BfaI CTAG 7 cut(s) 296, 675, 711, 746, 783, 893, 1064
BfmI CTRYAG 2 cut(s) 988, 1068
BisI GCNGC 5 cut(s) 231, 234, 252, 426, 1068
BlsI GCNGC 5 cut(s) 232, 235, 253, 427, 1069
Bme1390I CCNGG 2 cut(s) 386, 973
BmiI GGNNCC 2 cut(s) 245, 469
BmrFI CCNGG 2 cut(s) 386, 973
Bpu10I CCTNAGC 2 cut(s) 186, 843
Bpu14I TTCGAA 1 cut(s) 515
BsaJI CCNNGG 2 cut(s) 420, 971
Bsc4I CCNNNNNNNGG 1 cut(s) 977
BseBI CCWGG 2 cut(s) 386, 973
BseDI CCNNGG 2 cut(s) 420, 971
BseGI GGATG 3 cut(s) 125, 452, 720
BseLI CCNNNNNNNGG 1 cut(s) 977
BseMII CTCAG 4 cut(s) 69, 200, 213, 911
BseSI GKGCMC 1 cut(s) 1113
BseXI GCAGC 5 cut(s) 217, 220, 263, 437, 1054
Bsh1236I CGCG 1 cut(s) 1154
BshFI GGCC 4 cut(s) 419, 715, 794, 815
BsiHKAI GWGCWC 3 cut(s) 265, 397, 1113
BslFI GGGAC 1 cut(s) 663
BslI CCNNNNNNNGG 1 cut(s) 977
BsmAI GTCTC 2 cut(s) 352, 916
BsmBI CGTCTC 2 cut(s) 352, 916
BsmFI GGGAC 1 cut(s) 663
BsmI GAATGC 2 cut(s) 232, 690
BsnI GGCC 4 cut(s) 419, 715, 794, 815
Bsp119I TTCGAA 1 cut(s) 515
Bsp1286I GDGCHC 3 cut(s) 265, 397, 1113
Bsp143I GATC 8 cut(s) 122, 243, 574, 586, 613, 952, 967, 1089
BspANI GGCC 4 cut(s) 419, 715, 794, 815
BspCNI CTCAG 4 cut(s) 70, 199, 214, 912
BspFNI CGCG 1 cut(s) 1154
BspLI GGNNCC 2 cut(s) 245, 469
BspMAI CTGCAG 1 cut(s) 1072
BspPI GGATC 4 cut(s) 238, 251, 962, 1097
BspT104I TTCGAA 1 cut(s) 515
BssECI CCNNGG 2 cut(s) 420, 971
BssMI GATC 8 cut(s) 122, 243, 574, 586, 613, 952, 967, 1089
BssNAI GTATAC 1 cut(s) 1053
Bst1107I GTATAC 1 cut(s) 1053
Bst2UI CCWGG 2 cut(s) 386, 973
Bst4CI ACNGT 2 cut(s) 915, 939
Bst6I CTCTTC 3 cut(s) 284, 457, 523
BstBI TTCGAA 1 cut(s) 515
BstC8I GCNNGC 5 cut(s) 13, 365, 369, 397, 881
BstDEI CTNAG 5 cut(s) 78, 186, 222, 843, 920
BstF5I GGATG 3 cut(s) 125, 452, 720
BstFNI CGCG 1 cut(s) 1154
BstKTI GATC 8 cut(s) 125, 246, 577, 589, 616, 955, 970, 1092
BstMAI GTCTC 2 cut(s) 352, 916
BstMBI GATC 8 cut(s) 122, 243, 574, 586, 613, 952, 967, 1089
BstMWI GCNNNNNNNGC 6 cut(s) 181, 227, 260, 281, 377, 425
BstNI CCWGG 2 cut(s) 386, 973
BstNSI RCATGY 4 cut(s) 284, 367, 371, 762
BstSCI CCNGG 2 cut(s) 384, 971
BstSFI CTRYAG 2 cut(s) 988, 1068
BstSLI GKGCMC 1 cut(s) 1113
BstUI CGCG 1 cut(s) 1154
BstV1I GCAGC 5 cut(s) 217, 220, 263, 437, 1054
BstX2I RGATCY 2 cut(s) 243, 1089
BstYI RGATCY 2 cut(s) 243, 1089
BstZ17I GTATAC 1 cut(s) 1053
BsuRI GGCC 4 cut(s) 419, 715, 794, 815
BtsCI GGATG 3 cut(s) 125, 452, 720
Cac8I GCNNGC 5 cut(s) 13, 365, 369, 397, 881
CaiI CAGNNNCTG 1 cut(s) 919
CseI GACGC 1 cut(s) 369
Csp6I GTAC 1 cut(s) 543
CspCI CAANNNNNGTGG 2 cut(s) 1114, 1149
CviQI GTAC 1 cut(s) 543
DdeI CTNAG 5 cut(s) 78, 186, 222, 843, 920
DpnI GATC 8 cut(s) 124, 245, 576, 588, 615, 954, 969, 1091
DpnII GATC 8 cut(s) 122, 243, 574, 586, 613, 952, 967, 1089
DraIII CACNNNGTG 2 cut(s) 331, 836
EaeI YGGCCR 3 cut(s) 417, 792, 813
Eam1104I CTCTTC 3 cut(s) 284, 457, 523
EarI CTCTTC 3 cut(s) 284, 457, 523
Ecl136II GAGCTC 1 cut(s) 395
Eco147I AGGCCT 1 cut(s) 715
Eco24I GRGCYC 1 cut(s) 397
Eco53kI GAGCTC 1 cut(s) 395
Eco57I CTGAAG 1 cut(s) 308
EcoICRI GAGCTC 1 cut(s) 395
EcoRI GAATTC 1 cut(s) 408
EcoRII CCWGG 2 cut(s) 384, 971
EcoT22I ATGCAT 1 cut(s) 369
EcoT38I GRGCYC 1 cut(s) 397
Esp3I CGTCTC 2 cut(s) 352, 916
FaqI GGGAC 1 cut(s) 663
FbaI TGATCA 3 cut(s) 574, 586, 952
FblI GTMKAC 1 cut(s) 1052
Fnu4HI GCNGC 5 cut(s) 231, 234, 252, 426, 1068
FokI GGATG 3 cut(s) 112, 439, 707
FriOI GRGCYC 1 cut(s) 397
Fsp4HI GCNGC 5 cut(s) 231, 234, 252, 426, 1068
FspBI CTAG 7 cut(s) 296, 675, 711, 746, 783, 893, 1064
GluI GCNGC 5 cut(s) 231, 234, 252, 426, 1068
HaeIII GGCC 4 cut(s) 419, 715, 794, 815
HgaI GACGC 1 cut(s) 369
HphI GGTGA 1 cut(s) 684
Hpy166II GTNNAC 3 cut(s) 839, 1053, 1111
Hpy188I TCNGA 8 cut(s) 27, 36, 405, 414, 579, 613, 662, 921
Hpy188III TCNNGA 1 cut(s) 525
Hpy8I GTNNAC 3 cut(s) 839, 1053, 1111
Hpy99I CGWCG 1 cut(s) 814
HpyAV CCTTC 2 cut(s) 265, 699
HpyCH4III ACNGT 2 cut(s) 915, 939
HpyCH4IV ACGT 1 cut(s) 925
HpyCH4V TGCA 3 cut(s) 367, 1070, 1111
HpyF10VI GCNNNNNNNGC 6 cut(s) 181, 227, 260, 281, 377, 425
HpyF3I CTNAG 5 cut(s) 78, 186, 222, 843, 920
HpySE526I ACGT 1 cut(s) 925
Ksp22I TGATCA 3 cut(s) 574, 586, 952
Kzo9I GATC 8 cut(s) 122, 243, 574, 586, 613, 952, 967, 1089
LmnI GCTCC 2 cut(s) 111, 861
Lsp1109I GCAGC 5 cut(s) 217, 220, 263, 437, 1054
MaeI CTAG 7 cut(s) 296, 675, 711, 746, 783, 893, 1064
MaeII ACGT 1 cut(s) 925
MaeIII GTNAC 5 cut(s) 303, 323, 548, 785, 828
MalI GATC 8 cut(s) 124, 245, 576, 588, 615, 954, 969, 1091
MboI GATC 8 cut(s) 122, 243, 574, 586, 613, 952, 967, 1089
MflI RGATCY 2 cut(s) 243, 1089
MhlI GDGCHC 3 cut(s) 265, 397, 1113
MlsI TGGCCA 1 cut(s) 794
MluCI AATT 2 cut(s) 170, 408
MluNI TGGCCA 1 cut(s) 794
MlyI GAGTC 1 cut(s) 301
MnlI CCTC 9 cut(s) 195, 314, 327, 415, 458, 483, 573, 1003, 1066
Mox20I TGGCCA 1 cut(s) 794
Mph1103I ATGCAT 1 cut(s) 369
MroXI GAANNNNTTC 4 cut(s) 466, 688, 915, 1120
MscI TGGCCA 1 cut(s) 794
MseI TTAA 2 cut(s) 99, 1032
MslI CAYNNNNRTG 1 cut(s) 480
Msp20I TGGCCA 1 cut(s) 794
MspA1I CMGCKG 1 cut(s) 190
MspR9I CCNGG 2 cut(s) 386, 973
Mva1269I GAATGC 2 cut(s) 232, 690
MvaI CCWGG 2 cut(s) 386, 973
MvnI CGCG 1 cut(s) 1154
MwoI GCNNNNNNNGC 6 cut(s) 181, 227, 260, 281, 377, 425
NdeII GATC 8 cut(s) 122, 243, 574, 586, 613, 952, 967, 1089
NlaIV GGNNCC 2 cut(s) 245, 469
NmeAIII GCCGAG 1 cut(s) 445
NmuCI GTSAC 3 cut(s) 323, 548, 828
NsiI ATGCAT 1 cut(s) 369
NspI RCATGY 4 cut(s) 284, 367, 371, 762
NspV TTCGAA 1 cut(s) 515
PaeI GCATGC 2 cut(s) 367, 371
PceI AGGCCT 1 cut(s) 715
PciI ACATGT 1 cut(s) 758
PctI GAATGC 2 cut(s) 232, 690
PdmI GAANNNNTTC 4 cut(s) 466, 688, 915, 1120
PkrI GCNGC 5 cut(s) 232, 235, 253, 427, 1069
PleI GAGTC 1 cut(s) 300
PpsI GAGTC 1 cut(s) 300
PscI ACATGT 1 cut(s) 758
PshBI ATTAAT 1 cut(s) 1032
Psp124BI GAGCTC 1 cut(s) 397
Psp6I CCWGG 2 cut(s) 384, 971
PspGI CCWGG 2 cut(s) 384, 971
PspN4I GGNNCC 2 cut(s) 245, 469
PstI CTGCAG 1 cut(s) 1072
PstNI CAGNNNCTG 1 cut(s) 919
PsuI RGATCY 2 cut(s) 243, 1089
PvuII CAGCTG 1 cut(s) 190
RsaI GTAC 1 cut(s) 544
RsaNI GTAC 1 cut(s) 543
RseI CAYNNNNRTG 1 cut(s) 480
SacI GAGCTC 1 cut(s) 397
SaqAI TTAA 2 cut(s) 99, 1032
SatI GCNGC 5 cut(s) 231, 234, 252, 426, 1068
Sau3AI GATC 8 cut(s) 122, 243, 574, 586, 613, 952, 967, 1089
SchI GAGTC 1 cut(s) 301
ScrFI CCNGG 2 cut(s) 386, 973
SduI GDGCHC 3 cut(s) 265, 397, 1113
SfcI CTRYAG 2 cut(s) 988, 1068
SfuI TTCGAA 1 cut(s) 515
SmiMI CAYNNNNRTG 1 cut(s) 480
SpeI ACTAGT 2 cut(s) 674, 892
SphI GCATGC 2 cut(s) 367, 371
Sse9I AATT 2 cut(s) 170, 408
SseBI AGGCCT 1 cut(s) 715
SspI AATATT 1 cut(s) 1103
SspMI CTAG 7 cut(s) 296, 675, 711, 746, 783, 893, 1064
SstI GAGCTC 1 cut(s) 397
StuI AGGCCT 1 cut(s) 715
StyD4I CCNGG 2 cut(s) 384, 971
TaaI ACNGT 2 cut(s) 915, 939
TaiI ACGT 1 cut(s) 928
TaqI TCGA 5 cut(s) 155, 391, 515, 750, 809
TasI AATT 2 cut(s) 170, 408
TatI WGTACW 1 cut(s) 542
Tru1I TTAA 2 cut(s) 99, 1032
Tru9I TTAA 2 cut(s) 99, 1032
TseFI GTSAC 3 cut(s) 323, 548, 828
TseI GCWGC 5 cut(s) 230, 233, 251, 425, 1067
Tsp45I GTSAC 3 cut(s) 323, 548, 828
TspDTI ATGAA 8 cut(s) 78, 479, 498, 534, 663, 766, 918, 924
VneI GTGCAC 1 cut(s) 1109
VspI ATTAAT 1 cut(s) 1032
XapI RAATTY 1 cut(s) 408
XceI RCATGY 4 cut(s) 284, 367, 371, 762
XmiI GTMKAC 1 cut(s) 1052
XmnI GAANNNNTTC 4 cut(s) 466, 688, 915, 1120
XspI CTAG 7 cut(s) 296, 675, 711, 746, 783, 893, 1064
Zsp2I ATGCAT 1 cut(s) 369
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.