RLG00000025654

Protein of unknown function (DUF4005)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0015645
Physical Location & Seq
Reverse (-)
47381073 .. 47382691
1619 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025654

Sequence Viewer

Length: 1137 bp
ATGGGGAAGGCAAGCAAATGGATCCTGAACTTTCTGATAGGAAAGCAGGAGAAGAAGAAGAATGAAGCTCTTTTGACTGAGAAATGTGTGTCTTCTACCACCAAGCCAATCCACCCGGGGAGTCCTAAAGTTAAGCGAAGATGGAGCTTTGGAAGATCATCCCACAGGGTTTCAAAATCTGTTGATTCAATTGACACCACCAAATTGCCATTGAAGCCTCAGCTGAATCATGCCGTCTGGGTATCAATACAGGCTGAGAATGCTGCTGCTACAAGGATCCAAGCAGCCTTTCGTGCTCATTTGGCAAGGAAGGCTTTACATGCCCTGAAGAGTCTAGTGAAGTTACAAGCACTCATAAGAGGTCACTTTGTGAGGAAACAAACTAATGCTACTATGAGGCGCATGCATGCTTTGTTGGCAATCCAGGTCCGAGCTCGCTGTCAGAGAATTCAGATGGCTGAGGCGGCTGTACAGGTAGAAAATAAAAAGCAGTCATCCATTAGAAGAGGTTCCCCTCATGCCAATGAAGTTCATAGGAAGAAAATCTATATGATTTCCAATGAATCACAAAGAGTTTTGAAAAGTACAAGTGACTACCTGAATCATTCAAAAACAGATCAGAGGATTGATCATAGCTACACATCATTTTCTTCTGATCGTCTGTCCATCTCCAACCGACACAAATACAATGAAACCTTGTTCATAACACCAGAAACTAGTCCTCGAATGCCTTCACCCAAACCGAAACCAACAAGACCTTACATCCCTTTTCAACAGCATGATTCTCTAGTCGAGCCTACATGTTATGACTACTCATTCATGCCTAGTTACATGGCCAACACACAATCTTCAAGGGCCAAAAAAGTCAGGTCACAGAGTGAACCTAAGCAACGACCTGATGGGAGCAACGAGCATAAAAGCAAGCATACAGAACTAGTAGATTCAACAGATGAACAGTTTCTGAGACGTTCATCTTCACAGTTCAAAACCAATGATCACAAAAACCATGATCCCTGGTTTGGTAAGCTCTACAGACCAAGAAGATTGTTTGAGGACAACAAGTATGATTGCATTAATCCAAGTCATTCCAAGTATACTGAATCTCTAGCTGCAGAGCCCCATGTGATTTTGTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4005 IQ

Protein Analysis

379

Amino Acids

43.62

Weight (kDa)

10.18

Isoelectric Point (pI)

55.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4005 PF13178 266 - 315 3.4e-08 Protein of unknown function (DUF4005)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015645)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g03280
malus_domestica MD04G1222100.v1.1 MD12G1238300.v1.1
prunus_persica Prupe.6G341200_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0451751
rosa_laevigata RLG00000025654
rosa_multiflora Rmu_sc0004924.1_g000003 Rmu_sc0009360.1_g000003
rosa_roxburghii Rroxscaffold_164G00436340 Rroxscaffold_6G00428950
rosa_rugosa Rorug02G0635600
rosa_samantha Rh3AG037400 Rh3BG038500 Rh3CG037000 Rh3DG037700
rosa_wichuraiana Rw3G002790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1094
AciI CCGC 1 cut(s) 464
AclWI GGATC 5 cut(s) 16, 29, 271, 284, 1004
AcoI YGGCCR 1 cut(s) 834
AcsI RAATTY 1 cut(s) 447
AcuI CTGAAG 1 cut(s) 347
AdeI CACNNNGTG 2 cut(s) 370, 878
AfaI GTAC 2 cut(s) 471, 586
AfiI CCNNNNNNNGG 1 cut(s) 1019
AflIII ACRYGT 1 cut(s) 800
AgsI TTSAA 9 cut(s) 174, 189, 214, 580, 609, 773, 852, 945, 985
AhlI ACTAGT 2 cut(s) 716, 934
AjnI CCWGG 2 cut(s) 423, 1013
AjuI GAANNNNNNNTTGG 2 cut(s) 273, 305
AluBI AGCT 7 cut(s) 68, 147, 223, 434, 636, 1027, 1109
AluI AGCT 7 cut(s) 68, 147, 223, 434, 636, 1027, 1109
Alw21I GWGCWC 2 cut(s) 298, 436
Alw26I GTCTC 1 cut(s) 958
AlwI GGATC 5 cut(s) 16, 29, 271, 284, 1004
AlwNI CAGNNNCTG 1 cut(s) 961
Ama87I CYCGRG 1 cut(s) 115
AoxI GGCC 2 cut(s) 834, 855
ApeKI GCWGC 4 cut(s) 263, 266, 284, 1109
ApoI RAATTY 1 cut(s) 447
AseI ATTAAT 1 cut(s) 1074
Asp700I GAANNNNTTC 3 cut(s) 508, 730, 957
AspLEI GCGC 1 cut(s) 402
AspS9I GGNCC 2 cut(s) 427, 855
AsuC2I CCSGG 2 cut(s) 116, 117
AsuHPI GGTGA 1 cut(s) 726
AvaI CYCGRG 1 cut(s) 115
AvaII GGWCC 1 cut(s) 427
BalI TGGCCA 1 cut(s) 836
BamHI GGATCC 2 cut(s) 21, 276
BanII GRGCYC 2 cut(s) 436, 1119
BbsI GAAGAC 1 cut(s) 84
Bbv12I GWGCWC 2 cut(s) 298, 436
BbvCI CCTCAGC 2 cut(s) 219, 459
BbvI GCAGC 4 cut(s) 250, 253, 296, 1096
BccI CCATC 4 cut(s) 135, 448, 674, 893
BceAI ACGGC 1 cut(s) 218
BciT130I CCWGG 2 cut(s) 425, 1015
BclI TGATCA 2 cut(s) 628, 994
BcnI CCSGG 2 cut(s) 116, 117
BcoDI GTCTC 1 cut(s) 958
BcuI ACTAGT 2 cut(s) 716, 934
BfaI CTAG 6 cut(s) 335, 717, 788, 825, 935, 1106
BfmI CTRYAG 2 cut(s) 1030, 1110
BisI GCNGC 5 cut(s) 264, 267, 285, 465, 1110
BlsI GCNGC 5 cut(s) 265, 268, 286, 466, 1111
Bme1390I CCNGG 4 cut(s) 116, 117, 425, 1015
Bme18I GGWCC 1 cut(s) 427
BmeT110I CYCGRG 1 cut(s) 115
BmgT120I GGNCC 2 cut(s) 427, 855
BmiI GGNNCC 3 cut(s) 23, 278, 511
BmrFI CCNGG 4 cut(s) 116, 117, 425, 1015
BpiI GAAGAC 1 cut(s) 84
Bpu10I CCTNAGC 3 cut(s) 219, 459, 885
BpuMI CCSGG 2 cut(s) 116, 117
BsaJI CCNNGG 3 cut(s) 115, 116, 1013
Bsc4I CCNNNNNNNGG 1 cut(s) 1019
BseBI CCWGG 2 cut(s) 425, 1015
BseDI CCNNGG 3 cut(s) 115, 116, 1013
BseGI GGATG 3 cut(s) 158, 494, 762
BseLI CCNNNNNNNGG 1 cut(s) 1019
BseMII CTCAG 5 cut(s) 69, 233, 246, 450, 953
BseXI GCAGC 4 cut(s) 250, 253, 296, 1096
BshFI GGCC 2 cut(s) 836, 857
BsiHKAI GWGCWC 2 cut(s) 298, 436
BsiHKCI CYCGRG 1 cut(s) 115
BsiSI CCGG 1 cut(s) 116
BslI CCNNNNNNNGG 1 cut(s) 1019
BsmAI GTCTC 1 cut(s) 958
BsmBI CGTCTC 1 cut(s) 958
BsmI GAATGC 2 cut(s) 265, 732
BsnI GGCC 2 cut(s) 836, 857
BsoBI CYCGRG 1 cut(s) 115
Bsp1286I GDGCHC 3 cut(s) 298, 436, 1119
Bsp1407I TGTACA 1 cut(s) 469
Bsp143I GATC 8 cut(s) 21, 155, 276, 616, 628, 655, 994, 1009
BspACI CCGC 1 cut(s) 464
BspANI GGCC 2 cut(s) 836, 857
BspCNI CTCAG 5 cut(s) 70, 232, 247, 451, 954
BspLI GGNNCC 3 cut(s) 23, 278, 511
BspMAI CTGCAG 1 cut(s) 1114
BspPI GGATC 5 cut(s) 16, 29, 271, 284, 1004
BsrGI TGTACA 1 cut(s) 469
BssECI CCNNGG 3 cut(s) 115, 116, 1013
BssMI GATC 8 cut(s) 21, 155, 276, 616, 628, 655, 994, 1009
BssNAI GTATAC 1 cut(s) 1095
Bst1107I GTATAC 1 cut(s) 1095
Bst2UI CCWGG 2 cut(s) 425, 1015
Bst4CI ACNGT 2 cut(s) 957, 981
Bst6I CTCTTC 2 cut(s) 323, 499
BstAUI TGTACA 1 cut(s) 469
BstC8I GCNNGC 5 cut(s) 13, 404, 408, 436, 923
BstDEI CTNAG 6 cut(s) 78, 219, 255, 459, 885, 962
BstF5I GGATG 3 cut(s) 158, 494, 762
BstHHI GCGC 1 cut(s) 402
BstKTI GATC 8 cut(s) 24, 158, 279, 619, 631, 658, 997, 1012
BstMAI GTCTC 1 cut(s) 958
BstMBI GATC 8 cut(s) 21, 155, 276, 616, 628, 655, 994, 1009
BstMWI GCNNNNNNNGC 8 cut(s) 214, 260, 293, 302, 311, 320, 416, 464
BstNI CCWGG 2 cut(s) 425, 1015
BstNSI RCATGY 4 cut(s) 323, 406, 410, 804
BstSCI CCNGG 4 cut(s) 114, 115, 423, 1013
BstSFI CTRYAG 2 cut(s) 1030, 1110
BstV1I GCAGC 4 cut(s) 250, 253, 296, 1096
BstV2I GAAGAC 1 cut(s) 84
BstX2I RGATCY 2 cut(s) 21, 276
BstYI RGATCY 2 cut(s) 21, 276
BstZ17I GTATAC 1 cut(s) 1095
BsuRI GGCC 2 cut(s) 836, 857
BtsCI GGATG 3 cut(s) 158, 494, 762
Cac8I GCNNGC 5 cut(s) 13, 404, 408, 436, 923
CaiI CAGNNNCTG 1 cut(s) 961
CfoI GCGC 1 cut(s) 402
Cfr13I GGNCC 2 cut(s) 427, 855
Cfr9I CCCGGG 1 cut(s) 115
Csp6I GTAC 2 cut(s) 470, 585
CviQI GTAC 2 cut(s) 470, 585
DdeI CTNAG 6 cut(s) 78, 219, 255, 459, 885, 962
DpnI GATC 8 cut(s) 23, 157, 278, 618, 630, 657, 996, 1011
DpnII GATC 8 cut(s) 21, 155, 276, 616, 628, 655, 994, 1009
DraIII CACNNNGTG 2 cut(s) 370, 878
EaeI YGGCCR 1 cut(s) 834
Eam1104I CTCTTC 2 cut(s) 323, 499
EarI CTCTTC 2 cut(s) 323, 499
Ecl136II GAGCTC 1 cut(s) 434
Eco24I GRGCYC 2 cut(s) 436, 1119
Eco47I GGWCC 1 cut(s) 427
Eco53kI GAGCTC 1 cut(s) 434
Eco57I CTGAAG 1 cut(s) 347
Eco88I CYCGRG 1 cut(s) 115
EcoICRI GAGCTC 1 cut(s) 434
EcoRI GAATTC 1 cut(s) 447
EcoRII CCWGG 2 cut(s) 423, 1013
EcoT22I ATGCAT 1 cut(s) 408
EcoT38I GRGCYC 2 cut(s) 436, 1119
Esp3I CGTCTC 1 cut(s) 958
FalI AAGNNNNNCTT 2 cut(s) 298, 330
FbaI TGATCA 2 cut(s) 628, 994
FblI GTMKAC 1 cut(s) 1094
Fnu4HI GCNGC 5 cut(s) 264, 267, 285, 465, 1110
FokI GGATG 3 cut(s) 145, 481, 749
FriOI GRGCYC 2 cut(s) 436, 1119
Fsp4HI GCNGC 5 cut(s) 264, 267, 285, 465, 1110
FspBI CTAG 6 cut(s) 335, 717, 788, 825, 935, 1106
GlaI GCGC 1 cut(s) 401
GluI GCNGC 5 cut(s) 264, 267, 285, 465, 1110
HaeIII GGCC 2 cut(s) 836, 857
HapII CCGG 1 cut(s) 116
HhaI GCGC 1 cut(s) 402
Hin6I GCGC 1 cut(s) 400
HinP1I GCGC 1 cut(s) 400
HinfI GANTC 9 cut(s) 121, 185, 226, 331, 563, 601, 782, 941, 1100
HpaII CCGG 1 cut(s) 116
HphI GGTGA 1 cut(s) 726
Hpy166II GTNNAC 2 cut(s) 881, 1095
Hpy188I TCNGA 7 cut(s) 36, 431, 444, 453, 621, 655, 963
Hpy188III TCNNGA 1 cut(s) 25
Hpy8I GTNNAC 2 cut(s) 881, 1095
HpyAV CCTTC 2 cut(s) 304, 741
HpyCH4III ACNGT 2 cut(s) 957, 981
HpyCH4IV ACGT 1 cut(s) 967
HpyCH4V TGCA 3 cut(s) 406, 1071, 1112
HpyF10VI GCNNNNNNNGC 8 cut(s) 214, 260, 293, 302, 311, 320, 416, 464
HpyF3I CTNAG 6 cut(s) 78, 219, 255, 459, 885, 962
HpySE526I ACGT 1 cut(s) 967
HspAI GCGC 1 cut(s) 400
Ksp22I TGATCA 2 cut(s) 628, 994
Kzo9I GATC 8 cut(s) 21, 155, 276, 616, 628, 655, 994, 1009
LmnI GCTCC 2 cut(s) 144, 903
Lsp1109I GCAGC 4 cut(s) 250, 253, 296, 1096
MaeI CTAG 6 cut(s) 335, 717, 788, 825, 935, 1106
MaeII ACGT 1 cut(s) 967
MaeIII GTNAC 5 cut(s) 342, 362, 590, 827, 870
MalI GATC 8 cut(s) 23, 157, 278, 618, 630, 657, 996, 1011
MboI GATC 8 cut(s) 21, 155, 276, 616, 628, 655, 994, 1009
MfeI CAATTG 1 cut(s) 189
MflI RGATCY 2 cut(s) 21, 276
MhlI GDGCHC 3 cut(s) 298, 436, 1119
MlsI TGGCCA 1 cut(s) 836
MluCI AATT 3 cut(s) 189, 203, 447
MluNI TGGCCA 1 cut(s) 836
MlyI GAGTC 2 cut(s) 130, 340
MmeI TCCRAC 1 cut(s) 696
Mox20I TGGCCA 1 cut(s) 836
Mph1103I ATGCAT 1 cut(s) 408
MroXI GAANNNNTTC 3 cut(s) 508, 730, 957
MscI TGGCCA 1 cut(s) 836
MseI TTAA 3 cut(s) 132, 1074, 1135
MslI CAYNNNNRTG 1 cut(s) 522
Msp20I TGGCCA 1 cut(s) 836
MspA1I CMGCKG 1 cut(s) 223
MspI CCGG 1 cut(s) 116
MspR9I CCNGG 4 cut(s) 116, 117, 425, 1015
MunI CAATTG 1 cut(s) 189
Mva1269I GAATGC 2 cut(s) 265, 732
MvaI CCWGG 2 cut(s) 425, 1015
MwoI GCNNNNNNNGC 8 cut(s) 214, 260, 293, 302, 311, 320, 416, 464
NciI CCSGG 2 cut(s) 116, 117
NdeII GATC 8 cut(s) 21, 155, 276, 616, 628, 655, 994, 1009
NlaIV GGNNCC 3 cut(s) 23, 278, 511
NmuCI GTSAC 3 cut(s) 362, 590, 870
NsiI ATGCAT 1 cut(s) 408
NspI RCATGY 4 cut(s) 323, 406, 410, 804
PaeI GCATGC 2 cut(s) 406, 410
PciI ACATGT 1 cut(s) 800
PctI GAATGC 2 cut(s) 265, 732
PdmI GAANNNNTTC 3 cut(s) 508, 730, 957
PfeI GAWTC 7 cut(s) 185, 226, 563, 601, 782, 941, 1100
PkrI GCNGC 5 cut(s) 265, 268, 286, 466, 1111
PleI GAGTC 2 cut(s) 129, 339
PpsI GAGTC 2 cut(s) 129, 339
PscI ACATGT 1 cut(s) 800
PshBI ATTAAT 1 cut(s) 1074
Psp124BI GAGCTC 1 cut(s) 436
Psp6I CCWGG 2 cut(s) 423, 1013
PspGI CCWGG 2 cut(s) 423, 1013
PspN4I GGNNCC 3 cut(s) 23, 278, 511
PspPI GGNCC 2 cut(s) 427, 855
PstI CTGCAG 1 cut(s) 1114
PstNI CAGNNNCTG 1 cut(s) 961
PsuI RGATCY 2 cut(s) 21, 276
PvuII CAGCTG 1 cut(s) 223
RsaI GTAC 2 cut(s) 471, 586
RsaNI GTAC 2 cut(s) 470, 585
RseI CAYNNNNRTG 1 cut(s) 522
SacI GAGCTC 1 cut(s) 436
SaqAI TTAA 3 cut(s) 132, 1074, 1135
SatI GCNGC 5 cut(s) 264, 267, 285, 465, 1110
Sau3AI GATC 8 cut(s) 21, 155, 276, 616, 628, 655, 994, 1009
Sau96I GGNCC 2 cut(s) 427, 855
SchI GAGTC 2 cut(s) 130, 340
ScrFI CCNGG 4 cut(s) 116, 117, 425, 1015
SduI GDGCHC 3 cut(s) 298, 436, 1119
SfcI CTRYAG 2 cut(s) 1030, 1110
SinI GGWCC 1 cut(s) 427
SmaI CCCGGG 1 cut(s) 117
SmiMI CAYNNNNRTG 1 cut(s) 522
SpeI ACTAGT 2 cut(s) 716, 934
SphI GCATGC 2 cut(s) 406, 410
Sse9I AATT 3 cut(s) 189, 203, 447
SsiI CCGC 1 cut(s) 464
SspMI CTAG 6 cut(s) 335, 717, 788, 825, 935, 1106
SstI GAGCTC 1 cut(s) 436
StyD4I CCNGG 4 cut(s) 114, 115, 423, 1013
TaaI ACNGT 2 cut(s) 957, 981
TaiI ACGT 1 cut(s) 970
TaqI TCGA 2 cut(s) 724, 792
TasI AATT 3 cut(s) 189, 203, 447
TatI WGTACW 2 cut(s) 469, 584
TauI GCSGC 1 cut(s) 467
TfiI GAWTC 7 cut(s) 185, 226, 563, 601, 782, 941, 1100
Tru1I TTAA 3 cut(s) 132, 1074, 1135
Tru9I TTAA 3 cut(s) 132, 1074, 1135
TseFI GTSAC 3 cut(s) 362, 590, 870
TseI GCWGC 4 cut(s) 263, 266, 284, 1109
Tsp45I GTSAC 3 cut(s) 362, 590, 870
TspDTI ATGAA 9 cut(s) 78, 521, 540, 576, 691, 705, 808, 960, 966
TspMI CCCGGG 1 cut(s) 115
VpaK11BI GGWCC 1 cut(s) 427
VspI ATTAAT 1 cut(s) 1074
XapI RAATTY 1 cut(s) 447
XceI RCATGY 4 cut(s) 323, 406, 410, 804
XmaI CCCGGG 1 cut(s) 115
XmiI GTMKAC 1 cut(s) 1094
XmnI GAANNNNTTC 3 cut(s) 508, 730, 957
XspI CTAG 6 cut(s) 335, 717, 788, 825, 935, 1106
Zsp2I ATGCAT 1 cut(s) 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.