MD04G1222100.v1.1

Protein of unknown function (DUF4005)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0015645
Physical Location & Seq
Reverse (-)
30312263 .. 30314147
1885 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1222100.v1.1.491

Sequence Viewer

Length: 1173 bp
ATGGGGAAGGCAAGTAGATGGATCATAAAGTTCTTGGTTGGAAAGCAAGAAAAAAAGATTAACGATGCTCCGAAAACTAAGGTTTCGAATGAGTCTTCATCTACCACTTCTGCCAGGCCGTCGGTGAACCGGCCAGGGACGCCGAAAGTGAAGAGAAGGTGGAGCTTTGGAAAGTTGGCAGGCAAAGAGGCGTCCCACAGGGTTACAAGATCTGTTGATTCAATTGACATCTCTAAGTTGTCACAGAAAGTTCAGAAGAATCATGCCGTGGCACCAAGAGATGTCGAAAATGCTGCTGCCACAAGGATTCAAGCTGCCTTCCGTTCGCATTTGGCAAGGAAGGCTTTACATGCCTTGAAGGGTTTGGTGAAGTTGCAAGCACTCATAAGAGGTCACATTGTGAGGAAACAAACAACTGCTACGATAATCCAGATGCAGGCGTTGATGGCGATCCAGGTTAGAGCGCGGTTTAAGCGAATTCAGATGGTGGAAGAGACACAGAGAGCTAATAAAAAACAGCTGATCATTCGAAGAGGCCACCCTCTGGACAGTGGAGTTCATAGGAAGTCAATAGATTTGAACCCGAATGATGTTCAACGAGTTGCGAAAAGCACGAGTGGACACCTTAATCATTCACAAGCTGCAGGGAGGTTTGAGCATGGCTGCTCAACAGTCTATTCAGATCGTCTTTCCATCTCAAACCGGCATCACTACGAGGAATCCTCTTTTGCAACAGTAAATAGTCCCTGGAATTATCTTGCTGCATCCAAACCAAAACCAAGAAGACACTCTTTCGCTCATCAACCCCAAGATGACACAGACTACGAGGACTTCGCATTCAAGCCAAATTACATGACCAACACGAAATCTTCCAAGGCGAAAGCTAGGTCACAGAGTGAACCAAAGCAGAGGCCTGAAGGAAGCTTGAAGAACAGAAGCAAGCAAATAGAAGTGGATGCAACACTAGTTGCCATGGAGGATCAAGTGCTGAAACGTTCATCTACGCAGTTCAAACCCAACGGTCAGAAAAGCCATGATCCCTGGTTTGTTAAGCTCTACAGATCAAGAAGATTGTTCGAGGACAACAAGCATGCTTCCGTTAGCTCGAGTACTGTCCATTCCTACTATCATGAATCTCTTGCTGCATATGAGCCCCATGTGAATTTGTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4005 IQ

Protein Analysis

391

Amino Acids

44.23

Weight (kDa)

10.43

Isoelectric Point (pI)

47.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4005 PF13178 269 - 322 1.7e-09 Protein of unknown function (DUF4005)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015645)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g03280
malus_domestica MD04G1222100.v1.1 MD12G1238300.v1.1
prunus_persica Prupe.6G341200_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0451751
rosa_laevigata RLG00000025654
rosa_multiflora Rmu_sc0004924.1_g000003 Rmu_sc0009360.1_g000003
rosa_roxburghii Rroxscaffold_164G00436340 Rroxscaffold_6G00428950
rosa_rugosa Rorug02G0635600
rosa_samantha Rh3AG037400 Rh3BG038500 Rh3CG037000 Rh3DG037700
rosa_wichuraiana Rw3G002790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 271
AccB7I CCANNNNNTGG 1 cut(s) 544
AccII CGCG 1 cut(s) 466
AciI CCGC 1 cut(s) 466
AclI AACGTT 1 cut(s) 994
AclWI GGATC 4 cut(s) 29, 445, 987, 1031
AcoI YGGCCR 1 cut(s) 131
AcsI RAATTY 2 cut(s) 477, 1162
AcuI CTGAAG 1 cut(s) 936
AcyI GRCGYC 2 cut(s) 140, 191
AdeI CACNNNGTG 2 cut(s) 400, 896
AfaI GTAC 2 cut(s) 1111, 1169
AfiI CCNNNNNNNGG 2 cut(s) 436, 544
AgsI TTSAA 8 cut(s) 222, 311, 358, 580, 596, 841, 928, 1012
AhlI ACTAGT 1 cut(s) 964
AjnI CCWGG 5 cut(s) 113, 133, 453, 746, 1040
AluBI AGCT 9 cut(s) 165, 314, 506, 520, 641, 884, 924, 1054, 1104
AluI AGCT 9 cut(s) 165, 314, 506, 520, 641, 884, 924, 1054, 1104
Alw26I GTCTC 1 cut(s) 488
AlwI GGATC 4 cut(s) 29, 445, 987, 1031
Ama87I CYCGRG 1 cut(s) 1105
AoxI GGCC 4 cut(s) 116, 131, 535, 911
ApeKI GCWGC 7 cut(s) 293, 296, 314, 641, 663, 761, 1142
ApoI RAATTY 2 cut(s) 477, 1162
ArsI GACNNNNNNTTYG 2 cut(s) 872, 904
AspLEI GCGC 1 cut(s) 466
AsuHPI GGTGA 2 cut(s) 136, 379
AsuII TTCGAA 2 cut(s) 86, 529
AvaI CYCGRG 1 cut(s) 1105
BanI GGYRCC 1 cut(s) 271
BanII GRGCYC 1 cut(s) 1155
BauI CACGAG 1 cut(s) 613
BbsI GAAGAC 2 cut(s) 87, 790
BbvI GCAGC 7 cut(s) 280, 283, 301, 628, 650, 748, 1129
BccI CCATC 4 cut(s) 12, 439, 478, 701
BceAI ACGGC 2 cut(s) 103, 251
BcgI CGANNNNNNTGC 2 cut(s) 275, 309
BciT130I CCWGG 5 cut(s) 115, 135, 455, 748, 1042
BclI TGATCA 1 cut(s) 522
BcoDI GTCTC 1 cut(s) 488
BcuI ACTAGT 1 cut(s) 964
BfaI CTAG 2 cut(s) 885, 965
BfmI CTRYAG 2 cut(s) 642, 1057
BglII AGATCT 1 cut(s) 209
BisI GCNGC 7 cut(s) 294, 297, 315, 642, 664, 762, 1143
BlsI GCNGC 7 cut(s) 295, 298, 316, 643, 665, 763, 1144
BmcAI AGTACT 1 cut(s) 1111
Bme1390I CCNGG 5 cut(s) 115, 135, 455, 748, 1042
BmeT110I CYCGRG 1 cut(s) 1105
BmiI GGNNCC 1 cut(s) 273
BmrFI CCNGG 5 cut(s) 115, 135, 455, 748, 1042
BmsI GCATC 5 cut(s) 55, 423, 715, 773, 946
BpiI GAAGAC 2 cut(s) 87, 790
BplI GAGNNNNNCTC 2 cut(s) 707, 739
Bpu14I TTCGAA 2 cut(s) 86, 529
BsaBI GATNNNNATC 1 cut(s) 449
BsaHI GRCGYC 2 cut(s) 140, 191
BsaJI CCNNGG 6 cut(s) 134, 267, 746, 873, 972, 1040
Bsc4I CCNNNNNNNGG 2 cut(s) 436, 544
Bse118I RCCGGY 2 cut(s) 129, 702
Bse8I GATNNNNATC 1 cut(s) 449
BseBI CCWGG 5 cut(s) 115, 135, 455, 748, 1042
BseDI CCNNGG 6 cut(s) 134, 267, 746, 873, 972, 1040
BseGI GGATG 2 cut(s) 764, 961
BseJI GATNNNNATC 1 cut(s) 449
BseLI CCNNNNNNNGG 2 cut(s) 436, 544
BseXI GCAGC 7 cut(s) 280, 283, 301, 628, 650, 748, 1129
Bsh1236I CGCG 1 cut(s) 466
BshFI GGCC 4 cut(s) 118, 133, 537, 913
BshNI GGYRCC 1 cut(s) 271
BsiHKCI CYCGRG 1 cut(s) 1105
BsiSI CCGG 2 cut(s) 130, 703
BslFI GGGAC 3 cut(s) 151, 178, 729
BslI CCNNNNNNNGG 2 cut(s) 436, 544
BsmAI GTCTC 1 cut(s) 488
BsmFI GGGAC 3 cut(s) 151, 178, 729
BsmI GAATGC 1 cut(s) 836
BsnI GGCC 4 cut(s) 118, 133, 537, 913
BsoBI CYCGRG 1 cut(s) 1105
Bsp119I TTCGAA 2 cut(s) 86, 529
Bsp1286I GDGCHC 1 cut(s) 1155
Bsp143I GATC 8 cut(s) 21, 209, 450, 522, 682, 979, 1036, 1061
Bsp19I CCATGG 1 cut(s) 972
BspACI CCGC 1 cut(s) 466
BspANI GGCC 4 cut(s) 118, 133, 537, 913
BspFNI CGCG 1 cut(s) 466
BspHI TCATGA 1 cut(s) 1129
BspLI GGNNCC 1 cut(s) 273
BspMAI CTGCAG 1 cut(s) 646
BspPI GGATC 4 cut(s) 29, 445, 987, 1031
BspT104I TTCGAA 2 cut(s) 86, 529
BspT107I GGYRCC 1 cut(s) 271
BsrFI RCCGGY 2 cut(s) 129, 702
BssAI RCCGGY 2 cut(s) 129, 702
BssECI CCNNGG 6 cut(s) 134, 267, 746, 873, 972, 1040
BssMI GATC 8 cut(s) 21, 209, 450, 522, 682, 979, 1036, 1061
BssNI GRCGYC 2 cut(s) 140, 191
BssSI CACGAG 1 cut(s) 613
BssT1I CCWWGG 2 cut(s) 873, 972
Bst2BI CACGAG 1 cut(s) 613
Bst2UI CCWGG 5 cut(s) 115, 135, 455, 748, 1042
Bst4CI ACNGT 5 cut(s) 551, 673, 736, 1022, 1114
Bst6I CTCTTC 3 cut(s) 146, 486, 526
BstACI GRCGYC 2 cut(s) 140, 191
BstBI TTCGAA 2 cut(s) 86, 529
BstC8I GCNNGC 5 cut(s) 181, 378, 438, 941, 1092
BstDEI CTNAG 2 cut(s) 78, 234
BstDSI CCRYGG 2 cut(s) 267, 972
BstF5I GGATG 2 cut(s) 764, 961
BstFNI CGCG 1 cut(s) 466
BstHHI GCGC 1 cut(s) 466
BstKTI GATC 8 cut(s) 24, 212, 453, 525, 685, 982, 1039, 1064
BstMAI GTCTC 1 cut(s) 488
BstMBI GATC 8 cut(s) 21, 209, 450, 522, 682, 979, 1036, 1061
BstMWI GCNNNNNNNGC 5 cut(s) 139, 341, 350, 446, 472
BstNI CCWGG 5 cut(s) 115, 135, 455, 748, 1042
BstNSI RCATGY 2 cut(s) 353, 1094
BstSCI CCNGG 5 cut(s) 113, 133, 453, 746, 1040
BstSFI CTRYAG 2 cut(s) 642, 1057
BstUI CGCG 1 cut(s) 466
BstV1I GCAGC 7 cut(s) 280, 283, 301, 628, 650, 748, 1129
BstV2I GAAGAC 2 cut(s) 87, 790
BstX2I RGATCY 1 cut(s) 209
BstYI RGATCY 1 cut(s) 209
BsuRI GGCC 4 cut(s) 118, 133, 537, 913
BtgI CCRYGG 2 cut(s) 267, 972
BtsCI GGATG 2 cut(s) 764, 961
BtsIMutI CAGTG 1 cut(s) 556
Cac8I GCNNGC 5 cut(s) 181, 378, 438, 941, 1092
CciI TCATGA 1 cut(s) 1129
CfoI GCGC 1 cut(s) 466
Cfr10I RCCGGY 2 cut(s) 129, 702
CseI GACGC 2 cut(s) 148, 180
Csp6I GTAC 2 cut(s) 1110, 1168
CviAII CATG 9 cut(s) 263, 350, 659, 853, 973, 1034, 1091, 1130, 1157
CviQI GTAC 2 cut(s) 1110, 1168
DdeI CTNAG 2 cut(s) 78, 234
DpnI GATC 8 cut(s) 23, 211, 452, 524, 684, 981, 1038, 1063
DpnII GATC 8 cut(s) 21, 209, 450, 522, 682, 979, 1036, 1061
DraIII CACNNNGTG 2 cut(s) 400, 896
EaeI YGGCCR 1 cut(s) 131
Eam1104I CTCTTC 3 cut(s) 146, 486, 526
EarI CTCTTC 3 cut(s) 146, 486, 526
Eco130I CCWWGG 2 cut(s) 873, 972
Eco147I AGGCCT 1 cut(s) 913
Eco24I GRGCYC 1 cut(s) 1155
Eco57I CTGAAG 1 cut(s) 936
Eco88I CYCGRG 1 cut(s) 1105
EcoRI GAATTC 1 cut(s) 477
EcoRII CCWGG 5 cut(s) 113, 133, 453, 746, 1040
EcoT14I CCWWGG 2 cut(s) 873, 972
EcoT38I GRGCYC 1 cut(s) 1155
ErhI CCWWGG 2 cut(s) 873, 972
FaeI CATG 9 cut(s) 266, 353, 662, 856, 976, 1037, 1094, 1133, 1160
FalI AAGNNNNNCTT 4 cut(s) 328, 360, 775, 807
FaqI GGGAC 3 cut(s) 151, 178, 729
FatI CATG 9 cut(s) 262, 349, 658, 852, 972, 1033, 1090, 1129, 1156
FauNDI CATATG 1 cut(s) 1147
FbaI TGATCA 1 cut(s) 522
Fnu4HI GCNGC 7 cut(s) 294, 297, 315, 642, 664, 762, 1143
FokI GGATG 2 cut(s) 751, 968
FriOI GRGCYC 1 cut(s) 1155
Fsp4HI GCNGC 7 cut(s) 294, 297, 315, 642, 664, 762, 1143
FspBI CTAG 2 cut(s) 885, 965
GlaI GCGC 1 cut(s) 465
GluI GCNGC 7 cut(s) 294, 297, 315, 642, 664, 762, 1143
HaeIII GGCC 4 cut(s) 118, 133, 537, 913
HapII CCGG 2 cut(s) 130, 703
HgaI GACGC 2 cut(s) 148, 180
HhaI GCGC 1 cut(s) 466
Hin1I GRCGYC 2 cut(s) 140, 191
Hin1II CATG 9 cut(s) 266, 353, 662, 856, 976, 1037, 1094, 1133, 1160
Hin6I GCGC 1 cut(s) 464
HinP1I GCGC 1 cut(s) 464
HindIII AAGCTT 1 cut(s) 922
HinfI GANTC 6 cut(s) 92, 218, 259, 307, 719, 1133
HpaII CCGG 2 cut(s) 130, 703
HphI GGTGA 2 cut(s) 136, 379
Hpy166II GTNNAC 3 cut(s) 127, 620, 899
Hpy188I TCNGA 5 cut(s) 72, 255, 483, 682, 1026
Hpy188III TCNNGA 4 cut(s) 430, 545, 1065, 1130
Hpy8I GTNNAC 3 cut(s) 127, 620, 899
Hpy99I CGWCG 1 cut(s) 124
HpyAV CCTTC 5 cut(s) 150, 328, 334, 352, 911
HpyCH4III ACNGT 5 cut(s) 551, 673, 736, 1022, 1114
HpyCH4IV ACGT 1 cut(s) 994
HpyCH4V TGCA 7 cut(s) 376, 436, 644, 731, 764, 959, 1145
HpyF10VI GCNNNNNNNGC 5 cut(s) 139, 341, 350, 446, 472
HpyF3I CTNAG 2 cut(s) 78, 234
HpySE526I ACGT 1 cut(s) 994
Hsp92I GRCGYC 2 cut(s) 140, 191
Hsp92II CATG 9 cut(s) 266, 353, 662, 856, 976, 1037, 1094, 1133, 1160
HspAI GCGC 1 cut(s) 464
Ksp22I TGATCA 1 cut(s) 522
Kzo9I GATC 8 cut(s) 21, 209, 450, 522, 682, 979, 1036, 1061
LmnI GCTCC 2 cut(s) 73, 162
Lsp1109I GCAGC 7 cut(s) 280, 283, 301, 628, 650, 748, 1129
LweI GCATC 5 cut(s) 55, 423, 715, 773, 946
MaeI CTAG 2 cut(s) 885, 965
MaeII ACGT 1 cut(s) 994
MaeIII GTNAC 4 cut(s) 202, 240, 392, 888
MalI GATC 8 cut(s) 23, 211, 452, 524, 684, 981, 1038, 1063
MboI GATC 8 cut(s) 21, 209, 450, 522, 682, 979, 1036, 1061
MboII GAAGA 9 cut(s) 87, 163, 268, 503, 543, 795, 861, 940, 1080
MfeI CAATTG 1 cut(s) 222
MflI RGATCY 1 cut(s) 209
MhlI GDGCHC 1 cut(s) 1155
MluCI AATT 5 cut(s) 222, 477, 751, 847, 1162
MlyI GAGTC 1 cut(s) 101
MmeI TCCRAC 1 cut(s) 19
MseI TTAA 4 cut(s) 60, 471, 627, 1050
MspA1I CMGCKG 1 cut(s) 520
MspI CCGG 2 cut(s) 130, 703
MspR9I CCNGG 5 cut(s) 115, 135, 455, 748, 1042
MunI CAATTG 1 cut(s) 222
Mva1269I GAATGC 1 cut(s) 836
MvaI CCWGG 5 cut(s) 115, 135, 455, 748, 1042
MvnI CGCG 1 cut(s) 466
MwoI GCNNNNNNNGC 5 cut(s) 139, 341, 350, 446, 472
NcoI CCATGG 1 cut(s) 972
NdeI CATATG 1 cut(s) 1147
NdeII GATC 8 cut(s) 21, 209, 450, 522, 682, 979, 1036, 1061
NlaIII CATG 9 cut(s) 266, 353, 662, 856, 976, 1037, 1094, 1133, 1160
NlaIV GGNNCC 1 cut(s) 273
NmuCI GTSAC 3 cut(s) 240, 392, 888
NspI RCATGY 2 cut(s) 353, 1094
NspV TTCGAA 2 cut(s) 86, 529
PaeI GCATGC 1 cut(s) 1094
PaeR7I CTCGAG 1 cut(s) 1105
PagI TCATGA 1 cut(s) 1129
PceI AGGCCT 1 cut(s) 913
PctI GAATGC 1 cut(s) 836
PfeI GAWTC 5 cut(s) 218, 259, 307, 719, 1133
PflMI CCANNNNNTGG 1 cut(s) 544
PkrI GCNGC 7 cut(s) 295, 298, 316, 643, 665, 763, 1144
PleI GAGTC 1 cut(s) 100
PpsI GAGTC 1 cut(s) 100
Psp1406I AACGTT 1 cut(s) 994
Psp6I CCWGG 5 cut(s) 113, 133, 453, 746, 1040
PspGI CCWGG 5 cut(s) 113, 133, 453, 746, 1040
PspN4I GGNNCC 1 cut(s) 273
PspXI VCTCGAGB 1 cut(s) 1105
PstI CTGCAG 1 cut(s) 646
PsuI RGATCY 1 cut(s) 209
PvuII CAGCTG 1 cut(s) 520
RsaI GTAC 2 cut(s) 1111, 1169
RsaNI GTAC 2 cut(s) 1110, 1168
SaqAI TTAA 4 cut(s) 60, 471, 627, 1050
SatI GCNGC 7 cut(s) 294, 297, 315, 642, 664, 762, 1143
Sau3AI GATC 8 cut(s) 21, 209, 450, 522, 682, 979, 1036, 1061
ScaI AGTACT 1 cut(s) 1111
SchI GAGTC 1 cut(s) 101
ScrFI CCNGG 5 cut(s) 115, 135, 455, 748, 1042
SduI GDGCHC 1 cut(s) 1155
SfaNI GCATC 5 cut(s) 55, 423, 715, 773, 946
SfcI CTRYAG 2 cut(s) 642, 1057
Sfr274I CTCGAG 1 cut(s) 1105
SfuI TTCGAA 2 cut(s) 86, 529
SlaI CTCGAG 1 cut(s) 1105
SmlI CTYRAG 1 cut(s) 1105
SmoI CTYRAG 1 cut(s) 1105
SpeI ACTAGT 1 cut(s) 964
SphI GCATGC 1 cut(s) 1094
Sse9I AATT 5 cut(s) 222, 477, 751, 847, 1162
SseBI AGGCCT 1 cut(s) 913
SsiI CCGC 1 cut(s) 466
SspMI CTAG 2 cut(s) 885, 965
StuI AGGCCT 1 cut(s) 913
StyD4I CCNGG 5 cut(s) 113, 133, 453, 746, 1040
StyI CCWWGG 2 cut(s) 873, 972
TaaI ACNGT 5 cut(s) 551, 673, 736, 1022, 1114
TaiI ACGT 1 cut(s) 997
TaqI TCGA 5 cut(s) 86, 285, 529, 1077, 1106
TasI AATT 5 cut(s) 222, 477, 751, 847, 1162
TatI WGTACW 2 cut(s) 1109, 1167
TfiI GAWTC 5 cut(s) 218, 259, 307, 719, 1133
Tru1I TTAA 4 cut(s) 60, 471, 627, 1050
Tru9I TTAA 4 cut(s) 60, 471, 627, 1050
TscAI CASTG 1 cut(s) 556
TseFI GTSAC 3 cut(s) 240, 392, 888
TseI GCWGC 7 cut(s) 293, 296, 314, 641, 663, 761, 1142
Tsp45I GTSAC 3 cut(s) 240, 392, 888
TspDTI ATGAA 4 cut(s) 87, 548, 987, 1146
TspGWI ACGGA 2 cut(s) 311, 1087
TspRI CASTG 1 cut(s) 556
Van91I CCANNNNNTGG 1 cut(s) 544
XapI RAATTY 2 cut(s) 477, 1162
XceI RCATGY 2 cut(s) 353, 1094
XhoI CTCGAG 1 cut(s) 1105
XspI CTAG 2 cut(s) 885, 965
ZrmI AGTACT 1 cut(s) 1111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.