Rroxscaffold_164G00436340

Protein of unknown function (DUF4005)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000164
OG0015645
Physical Location & Seq
Reverse (-)
288513 .. 292571
4059 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_164G00436340.1

Sequence Viewer

Length: 1536 bp
ATGGGGAAGGCAAGCAAATGGATTCTGAACTTTCTGATAGGAAAGCAGGAGAAGAAGAAGAATGAAGCTCTTTTGACTGAGAAATGTGTGTCTTCTACCACCAAGCCAATCCACCCGGGGAGTCCCAAAGTTAAGCGAAGATGGAGCTTTGGAAGATCATCCCACAGGGTTTCAAAATCTGTTGATTCGATTGACACCACCAAATTGCCATTGAAGCCTCAGCTGAATCATGCCGTCTGGGTATCAACACAGGCTGAGAATGCTGCTGCTACAAGGATCCAAGCAGCCTTTCGTGCTCATTTGGCAAGGAAGGCTTTACATGCCCTGAAGAGTCTAGTGAAGTTACAAGCACTCATAAGAGGTCACTTTGTGAGGAAACAAACTAATGCTACTATGAGACGCATGCATGCTTTGTTGGCAATCCAGGTTCGAGCTCGCTGTCAGAGAATTCAGATGGCCGAGGCAGCTATACAGGAAAATAAAAAGCAGTCATCCATTAGAAGAGGTTCCCCTCATGCCAATGAAGTTCATAGGAAGAAAATCTATATGATTTCGAATGAATCACGAAGAGTTTTGAAAAGTACAAGTGACTACCTGAATCATTCACAAACTGATCAGAGGATTGATCATAGCTACACATCATTTTCTTCTGATCGTCTGTCCATCTCTAACCGACACAAATACAATGAAACCTTGTTCAGAACACCAGAAACTAGTCCCCGAATGCCTTCACCCAAACCAAAACCAACTAGGCCTTACATCCCTTTTCAACAGCATGATTCTCTAGTCGAGCCTACATGTTATGACTACTCATTCATGCCTAGTTACATGGCCAACACAGAATCTTCGACGGCCAAAAAAGTCAGGTCACAGAGTGAACCTAAGCAACGACCTGATGGGAGCAACAAGCATAAAAGCAAGCATACAGAACTAGTAGATTCAACAGATGAACAGTTTCTGAGACGTTCATCTTCACAGTTCAAAACCAATGATCACAAAAACCATGATCCCTGGTTTGGTAAGCTCTACAGACCAAGAAGATTGTTTGAGGACAACAAGTATGATTACATTAATCCAAGTCATTCCAACTCAAGCAGCGCTTCTAAAGCTTCAGCCCTCTGCTCCAATGATGAGGTGTCAAGCCTGATGCGGCTTGATGATGCTCCTCCAGCATCAGCAGCAGCAGCAGCTTGGGGAGCCTTCTTGCCCCGCTTTGCTGGCCGAATGGTGCTGGTGGCTTTCAAAGCTTCTGGTGCCTTCTTTGCCGGCATGGACATACTTCCGATCAGATTCAAACTGGCCCAGGTAATTGTAAACTACAATCCAAGTATCCAGGGTAATGATTTTGAACCTCCAATTATATTATCCAGACATATTCTTAAGCTGCGGTTCTCGAGGGAGAGGGTGAGTGGGAGTGGGAGTGCAGAGATAACGATGAGAAGGGTCATAGCTAGGTCTCCAACGGAAATGGACAAGTTACAGGTGAAGCTGGCTAGGTATCTGAGCAGCCCAATGGGTGTTCCCGAAGATAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4005 IQ

Protein Analysis

511

Amino Acids

57.98

Weight (kDa)

10.28

Isoelectric Point (pI)

54.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4005 PF13178 261 - 314 1.8e-08 Protein of unknown function (DUF4005)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015645)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g03280
malus_domestica MD04G1222100.v1.1 MD12G1238300.v1.1
prunus_persica Prupe.6G341200_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0451751
rosa_laevigata RLG00000025654
rosa_multiflora Rmu_sc0004924.1_g000003 Rmu_sc0009360.1_g000003
rosa_roxburghii Rroxscaffold_164G00436340 Rroxscaffold_6G00428950
rosa_rugosa Rorug02G0635600
rosa_samantha Rh3AG037400 Rh3BG038500 Rh3CG037000 Rh3DG037700
rosa_wichuraiana Rw3G002790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1253
AciI CCGC 3 cut(s) 1150, 1210, 1387
AclWI GGATC 3 cut(s) 271, 284, 1001
AcoI YGGCCR 4 cut(s) 456, 831, 852, 1219
AcsI RAATTY 1 cut(s) 447
AcuI CTGAAG 2 cut(s) 347, 1095
AdeI CACNNNGTG 2 cut(s) 370, 875
AfaI GTAC 1 cut(s) 583
AfeI AGCGCT 1 cut(s) 1099
AfiI CCNNNNNNNGG 1 cut(s) 1016
AflII CTTAAG 1 cut(s) 1379
AflIII ACRYGT 1 cut(s) 797
AgsI TTSAA 9 cut(s) 174, 214, 577, 770, 942, 982, 1243, 1294, 1349
AhlI ACTAGT 2 cut(s) 713, 931
AjnI CCWGG 4 cut(s) 423, 1010, 1302, 1332
AjuI GAANNNNNNNTTGG 2 cut(s) 273, 305
Alw21I GWGCWC 2 cut(s) 298, 436
Alw26I GTCTC 3 cut(s) 391, 955, 1461
AlwI GGATC 3 cut(s) 271, 284, 1001
AlwNI CAGNNNCTG 1 cut(s) 958
Ama87I CYCGRG 2 cut(s) 115, 1393
Aor51HI AGCGCT 1 cut(s) 1099
AoxI GGCC 6 cut(s) 456, 752, 831, 852, 1219, 1299
ApoI RAATTY 1 cut(s) 447
AseI ATTAAT 1 cut(s) 1071
Asp700I GAANNNNTTC 3 cut(s) 505, 727, 954
AspLEI GCGC 1 cut(s) 1100
AspS9I GGNCC 1 cut(s) 1300
AsuC2I CCSGG 2 cut(s) 116, 117
AsuHPI GGTGA 3 cut(s) 723, 1417, 1495
AsuII TTCGAA 1 cut(s) 554
AvaI CYCGRG 2 cut(s) 115, 1393
BalI TGGCCA 1 cut(s) 833
BamHI GGATCC 1 cut(s) 276
BanI GGYRCC 1 cut(s) 1253
BanII GRGCYC 1 cut(s) 436
BbsI GAAGAC 1 cut(s) 84
Bbv12I GWGCWC 2 cut(s) 298, 436
BbvCI CCTCAGC 1 cut(s) 219
BccI CCATC 4 cut(s) 135, 448, 671, 890
BceAI ACGGC 2 cut(s) 218, 867
BciT130I CCWGG 4 cut(s) 425, 1012, 1304, 1334
BciVI GTATCC 1 cut(s) 1340
BclI TGATCA 3 cut(s) 613, 625, 991
BcnI CCSGG 2 cut(s) 116, 117
BcoDI GTCTC 3 cut(s) 391, 955, 1461
BcuI ACTAGT 2 cut(s) 713, 931
BfaI CTAG 8 cut(s) 335, 714, 750, 785, 822, 932, 1452, 1494
BfmI CTRYAG 1 cut(s) 1027
BfoI RGCGCY 1 cut(s) 1101
BfrI CTTAAG 1 cut(s) 1379
BfuI GTATCC 1 cut(s) 1340
Bme1390I CCNGG 6 cut(s) 116, 117, 425, 1012, 1304, 1334
BmeT110I CYCGRG 2 cut(s) 115, 1393
BmgT120I GGNCC 1 cut(s) 1300
BmiI GGNNCC 4 cut(s) 278, 508, 1198, 1255
BmrFI CCNGG 6 cut(s) 116, 117, 425, 1012, 1304, 1334
BmsI GCATC 3 cut(s) 1137, 1150, 1181
BpiI GAAGAC 1 cut(s) 84
BpmI CTGGAG 1 cut(s) 1152
Bpu10I CCTNAGC 2 cut(s) 219, 882
Bpu14I TTCGAA 1 cut(s) 554
BpuEI CTTGAG 1 cut(s) 1075
BpuMI CCSGG 2 cut(s) 116, 117
BsaI GGTCTC 1 cut(s) 1461
BsaJI CCNNGG 6 cut(s) 115, 116, 459, 1010, 1302, 1333
BsaXI ACNNNNNCTCC 2 cut(s) 1405, 1435
Bsc4I CCNNNNNNNGG 1 cut(s) 1016
Bse118I RCCGGY 1 cut(s) 1265
Bse1I ACTGG 1 cut(s) 1302
BseBI CCWGG 4 cut(s) 425, 1012, 1304, 1334
BseDI CCNNGG 6 cut(s) 115, 116, 459, 1010, 1302, 1333
BseGI GGATG 3 cut(s) 158, 491, 759
BseLI CCNNNNNNNGG 1 cut(s) 1016
BseMII CTCAG 5 cut(s) 69, 233, 246, 950, 1493
BseNI ACTGG 1 cut(s) 1302
BseRI GAGGAG 1 cut(s) 1155
BsgI GTGCAG 1 cut(s) 1443
BshFI GGCC 6 cut(s) 458, 754, 833, 854, 1221, 1301
BshNI GGYRCC 1 cut(s) 1253
BsiHKAI GWGCWC 2 cut(s) 298, 436
BsiHKCI CYCGRG 2 cut(s) 115, 1393
BsiSI CCGG 2 cut(s) 116, 1266
BslFI GGGAC 2 cut(s) 108, 702
BslI CCNNNNNNNGG 1 cut(s) 1016
BsmAI GTCTC 3 cut(s) 391, 955, 1461
BsmBI CGTCTC 2 cut(s) 391, 955
BsmFI GGGAC 2 cut(s) 108, 702
BsmI GAATGC 2 cut(s) 265, 729
BsnI GGCC 6 cut(s) 458, 754, 833, 854, 1221, 1301
Bso31I GGTCTC 1 cut(s) 1461
BsoBI CYCGRG 2 cut(s) 115, 1393
Bsp119I TTCGAA 1 cut(s) 554
Bsp1286I GDGCHC 2 cut(s) 298, 436
Bsp143I GATC 8 cut(s) 155, 276, 613, 625, 652, 991, 1006, 1284
BspACI CCGC 3 cut(s) 1150, 1210, 1387
BspANI GGCC 6 cut(s) 458, 754, 833, 854, 1221, 1301
BspCNI CTCAG 5 cut(s) 70, 232, 247, 951, 1494
BspLI GGNNCC 4 cut(s) 278, 508, 1198, 1255
BspPI GGATC 3 cut(s) 271, 284, 1001
BspT104I TTCGAA 1 cut(s) 554
BspT107I GGYRCC 1 cut(s) 1253
BspTI CTTAAG 1 cut(s) 1379
BspTNI GGTCTC 1 cut(s) 1461
BsrFI RCCGGY 1 cut(s) 1265
BsrI ACTGG 1 cut(s) 1302
BssAI RCCGGY 1 cut(s) 1265
BssECI CCNNGG 6 cut(s) 115, 116, 459, 1010, 1302, 1333
BssMI GATC 8 cut(s) 155, 276, 613, 625, 652, 991, 1006, 1284
Bst2UI CCWGG 4 cut(s) 425, 1012, 1304, 1334
Bst4CI ACNGT 2 cut(s) 954, 978
Bst6I CTCTTC 3 cut(s) 323, 496, 562
BstAFI CTTAAG 1 cut(s) 1379
BstBI TTCGAA 1 cut(s) 554
BstC8I GCNNGC 8 cut(s) 13, 404, 408, 436, 920, 1219, 1267, 1491
BstDEI CTNAG 6 cut(s) 78, 219, 255, 882, 959, 1502
BstF5I GGATG 3 cut(s) 158, 491, 759
BstH2I RGCGCY 1 cut(s) 1101
BstHHI GCGC 1 cut(s) 1100
BstKTI GATC 8 cut(s) 158, 279, 616, 628, 655, 994, 1009, 1287
BstMAI GTCTC 3 cut(s) 391, 955, 1461
BstMBI GATC 8 cut(s) 155, 276, 613, 625, 652, 991, 1006, 1284
BstNI CCWGG 4 cut(s) 425, 1012, 1304, 1334
BstNSI RCATGY 4 cut(s) 323, 406, 410, 801
BstSCI CCNGG 6 cut(s) 114, 115, 423, 1010, 1302, 1332
BstSFI CTRYAG 1 cut(s) 1027
BstV2I GAAGAC 1 cut(s) 84
BstX2I RGATCY 1 cut(s) 276
BstYI RGATCY 1 cut(s) 276
BsuI GTATCC 1 cut(s) 1340
BsuRI GGCC 6 cut(s) 458, 754, 833, 854, 1221, 1301
BtsCI GGATG 3 cut(s) 158, 491, 759
Cac8I GCNNGC 8 cut(s) 13, 404, 408, 436, 920, 1219, 1267, 1491
CaiI CAGNNNCTG 1 cut(s) 958
CfoI GCGC 1 cut(s) 1100
Cfr10I RCCGGY 1 cut(s) 1265
Cfr13I GGNCC 1 cut(s) 1300
Cfr9I CCCGGG 1 cut(s) 115
CseI GACGC 1 cut(s) 408
Csp6I GTAC 1 cut(s) 582
CviQI GTAC 1 cut(s) 582
DdeI CTNAG 6 cut(s) 78, 219, 255, 882, 959, 1502
DpnI GATC 8 cut(s) 157, 278, 615, 627, 654, 993, 1008, 1286
DpnII GATC 8 cut(s) 155, 276, 613, 625, 652, 991, 1006, 1284
DraIII CACNNNGTG 2 cut(s) 370, 875
EaeI YGGCCR 4 cut(s) 456, 831, 852, 1219
Eam1104I CTCTTC 3 cut(s) 323, 496, 562
EarI CTCTTC 3 cut(s) 323, 496, 562
Ecl136II GAGCTC 1 cut(s) 434
Eco147I AGGCCT 1 cut(s) 754
Eco24I GRGCYC 1 cut(s) 436
Eco31I GGTCTC 1 cut(s) 1461
Eco47III AGCGCT 1 cut(s) 1099
Eco53kI GAGCTC 1 cut(s) 434
Eco57I CTGAAG 2 cut(s) 347, 1095
Eco88I CYCGRG 2 cut(s) 115, 1393
EcoICRI GAGCTC 1 cut(s) 434
EcoRI GAATTC 1 cut(s) 447
EcoRII CCWGG 4 cut(s) 423, 1010, 1302, 1332
EcoT22I ATGCAT 1 cut(s) 408
EcoT38I GRGCYC 1 cut(s) 436
Esp3I CGTCTC 2 cut(s) 391, 955
FalI AAGNNNNNCTT 4 cut(s) 298, 330, 1084, 1116
FaqI GGGAC 2 cut(s) 108, 702
FauI CCCGC 1 cut(s) 1217
FbaI TGATCA 3 cut(s) 613, 625, 991
FokI GGATG 3 cut(s) 145, 478, 746
FriOI GRGCYC 1 cut(s) 436
FspBI CTAG 8 cut(s) 335, 714, 750, 785, 822, 932, 1452, 1494
GlaI GCGC 1 cut(s) 1099
GsuI CTGGAG 1 cut(s) 1152
HaeII RGCGCY 1 cut(s) 1101
HaeIII GGCC 6 cut(s) 458, 754, 833, 854, 1221, 1301
HapII CCGG 2 cut(s) 116, 1266
HgaI GACGC 1 cut(s) 408
HhaI GCGC 1 cut(s) 1100
Hin6I GCGC 1 cut(s) 1098
HinP1I GCGC 1 cut(s) 1098
HindIII AAGCTT 2 cut(s) 1107, 1245
HpaII CCGG 2 cut(s) 116, 1266
HphI GGTGA 3 cut(s) 723, 1417, 1495
Hpy166II GTNNAC 2 cut(s) 878, 1315
Hpy188III TCNNGA 4 cut(s) 564, 1368, 1393, 1523
Hpy8I GTNNAC 2 cut(s) 878, 1315
Hpy99I CGWCG 1 cut(s) 853
HpyAV CCTTC 5 cut(s) 304, 738, 1210, 1267, 1434
HpyCH4III ACNGT 2 cut(s) 954, 978
HpyCH4IV ACGT 1 cut(s) 964
HpyCH4V TGCA 2 cut(s) 406, 1424
HpyF3I CTNAG 6 cut(s) 78, 219, 255, 882, 959, 1502
HpySE526I ACGT 1 cut(s) 964
HspAI GCGC 1 cut(s) 1098
KroI GCCGGC 1 cut(s) 1265
KroNI GCCGGC 1 cut(s) 1267
Ksp22I TGATCA 3 cut(s) 613, 625, 991
Kzo9I GATC 8 cut(s) 155, 276, 613, 625, 652, 991, 1006, 1284
LmnI GCTCC 5 cut(s) 144, 900, 1127, 1168, 1196
LweI GCATC 3 cut(s) 1137, 1150, 1181
MaeI CTAG 8 cut(s) 335, 714, 750, 785, 822, 932, 1452, 1494
MaeII ACGT 1 cut(s) 964
MaeIII GTNAC 6 cut(s) 342, 362, 587, 824, 867, 1476
MalI GATC 8 cut(s) 157, 278, 615, 627, 654, 993, 1008, 1286
MboI GATC 8 cut(s) 155, 276, 613, 625, 652, 991, 1006, 1284
MflI RGATCY 1 cut(s) 276
MhlI GDGCHC 2 cut(s) 298, 436
MlsI TGGCCA 1 cut(s) 833
MluCI AATT 5 cut(s) 203, 447, 1308, 1356, 1531
MluNI TGGCCA 1 cut(s) 833
MlyI GAGTC 2 cut(s) 130, 340
MmeI TCCRAC 2 cut(s) 1110, 1484
Mox20I TGGCCA 1 cut(s) 833
Mph1103I ATGCAT 1 cut(s) 408
MroNI GCCGGC 1 cut(s) 1265
MroXI GAANNNNTTC 3 cut(s) 505, 727, 954
MscI TGGCCA 1 cut(s) 833
MseI TTAA 4 cut(s) 132, 1071, 1380, 1534
MslI CAYNNNNRTG 1 cut(s) 519
Msp20I TGGCCA 1 cut(s) 833
MspA1I CMGCKG 1 cut(s) 223
MspCI CTTAAG 1 cut(s) 1379
MspI CCGG 2 cut(s) 116, 1266
MspR9I CCNGG 6 cut(s) 116, 117, 425, 1012, 1304, 1334
Mva1269I GAATGC 2 cut(s) 265, 729
MvaI CCWGG 4 cut(s) 425, 1012, 1304, 1334
NaeI GCCGGC 1 cut(s) 1267
NciI CCSGG 2 cut(s) 116, 117
NdeII GATC 8 cut(s) 155, 276, 613, 625, 652, 991, 1006, 1284
NgoMIV GCCGGC 1 cut(s) 1265
NlaIV GGNNCC 4 cut(s) 278, 508, 1198, 1255
NmeAIII GCCGAG 1 cut(s) 484
NmuCI GTSAC 3 cut(s) 362, 587, 867
NsiI ATGCAT 1 cut(s) 408
NspI RCATGY 4 cut(s) 323, 406, 410, 801
NspV TTCGAA 1 cut(s) 554
PaeI GCATGC 2 cut(s) 406, 410
PaeR7I CTCGAG 1 cut(s) 1393
PceI AGGCCT 1 cut(s) 754
PciI ACATGT 1 cut(s) 797
PctI GAATGC 2 cut(s) 265, 729
PdiI GCCGGC 1 cut(s) 1267
PdmI GAANNNNTTC 3 cut(s) 505, 727, 954
PfeI GAWTC 9 cut(s) 22, 185, 226, 560, 598, 779, 842, 938, 1290
PleI GAGTC 2 cut(s) 129, 339
PpsI GAGTC 2 cut(s) 129, 339
PscI ACATGT 1 cut(s) 797
PshBI ATTAAT 1 cut(s) 1071
Psp124BI GAGCTC 1 cut(s) 436
Psp6I CCWGG 4 cut(s) 423, 1010, 1302, 1332
PspGI CCWGG 4 cut(s) 423, 1010, 1302, 1332
PspN4I GGNNCC 4 cut(s) 278, 508, 1198, 1255
PspPI GGNCC 1 cut(s) 1300
PstNI CAGNNNCTG 1 cut(s) 958
PsuI RGATCY 1 cut(s) 276
PvuII CAGCTG 1 cut(s) 223
RsaI GTAC 1 cut(s) 583
RsaNI GTAC 1 cut(s) 582
RseI CAYNNNNRTG 1 cut(s) 519
SacI GAGCTC 1 cut(s) 436
SaqAI TTAA 4 cut(s) 132, 1071, 1380, 1534
Sau3AI GATC 8 cut(s) 155, 276, 613, 625, 652, 991, 1006, 1284
Sau96I GGNCC 1 cut(s) 1300
SchI GAGTC 2 cut(s) 130, 340
ScrFI CCNGG 6 cut(s) 116, 117, 425, 1012, 1304, 1334
SduI GDGCHC 2 cut(s) 298, 436
SfaNI GCATC 3 cut(s) 1137, 1150, 1181
SfcI CTRYAG 1 cut(s) 1027
Sfr274I CTCGAG 1 cut(s) 1393
SfuI TTCGAA 1 cut(s) 554
SlaI CTCGAG 1 cut(s) 1393
SmaI CCCGGG 1 cut(s) 117
SmiMI CAYNNNNRTG 1 cut(s) 519
SmlI CTYRAG 3 cut(s) 1090, 1379, 1393
SmoI CTYRAG 3 cut(s) 1090, 1379, 1393
SpeI ACTAGT 2 cut(s) 713, 931
SphI GCATGC 2 cut(s) 406, 410
Sse9I AATT 5 cut(s) 203, 447, 1308, 1356, 1531
SseBI AGGCCT 1 cut(s) 754
SsiI CCGC 3 cut(s) 1150, 1210, 1387
SspMI CTAG 8 cut(s) 335, 714, 750, 785, 822, 932, 1452, 1494
SstI GAGCTC 1 cut(s) 436
StuI AGGCCT 1 cut(s) 754
StyD4I CCNGG 6 cut(s) 114, 115, 423, 1010, 1302, 1332
TaaI ACNGT 2 cut(s) 954, 978
TaiI ACGT 1 cut(s) 967
TaqI TCGA 6 cut(s) 188, 430, 554, 789, 848, 1394
TasI AATT 5 cut(s) 203, 447, 1308, 1356, 1531
TatI WGTACW 1 cut(s) 581
TauI GCSGC 1 cut(s) 1153
TfiI GAWTC 9 cut(s) 22, 185, 226, 560, 598, 779, 842, 938, 1290
Tru1I TTAA 4 cut(s) 132, 1071, 1380, 1534
Tru9I TTAA 4 cut(s) 132, 1071, 1380, 1534
TseFI GTSAC 3 cut(s) 362, 587, 867
Tsp45I GTSAC 3 cut(s) 362, 587, 867
TspDTI ATGAA 8 cut(s) 78, 518, 537, 573, 702, 805, 957, 963
TspGWI ACGGA 1 cut(s) 1478
TspMI CCCGGG 1 cut(s) 115
Vha464I CTTAAG 1 cut(s) 1379
VspI ATTAAT 1 cut(s) 1071
XapI RAATTY 1 cut(s) 447
XceI RCATGY 4 cut(s) 323, 406, 410, 801
XhoI CTCGAG 1 cut(s) 1393
XmaI CCCGGG 1 cut(s) 115
XmnI GAANNNNTTC 3 cut(s) 505, 727, 954
XspI CTAG 8 cut(s) 335, 714, 750, 785, 822, 932, 1452, 1494
Zsp2I ATGCAT 1 cut(s) 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.