FvH4_6g03440

Uncharacterized conserved protein (DUF2358)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0013501
Physical Location & Seq
Forward (+)
1895566 .. 1898421
2856 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g03440.t1

Sequence Viewer

Length: 663 bp
ATGGCGGGAGTGTGCTGCTCTCCGAGAGTACGGATTCCTCTGCTCCCACACCCCACGAGAATCCACAAAATCCAGTGCTGCGCAAAAGCACCGGATAACAAACCCAAGAGAGAAACTCCGCCGATACTTAAAGTCGCAGTGACCGGAGCCACCGAGCTCCTCAGGGTTCTGTCTTCCTTCAACCCAACAACCAGATTGGAAAAAGCGGATGATAAACAAAGGGACGAGTTTTCGGTTATAGGAATAGATGATGTTGTGTCGATTCTAGAAGCTGATTACAAGAACGCGTATTTCGTGACAGGCATTTTCACGTCGGCAATTTATGCTGAAGATTGTGTCTTTGAGGATCCAACTATTAGATTTCAAGGTACGGAGTTGTATGCTCGCAACTTGAAATTGCTTGTTCCTTTCTTTGACTCGCCATCGATTGGATTGGAAAAGATCGAGAAGGGTATTGATTCTGAAGCAACCTTTGTTCTAGCAACATGGAAACTAAGGACCTACCTGAAGCTCCCATGGAGGCCTTTGATTTCTATTGATGGAAGTACGGTCTACAACTTGGATGATAAGTTTAAAATTGTAAGGCATGCTGAGAGCTGGAATGTTTCTGCAATTGAAGCAGTTGGCCAGATATTCAGACCTTCTGGTAGGTCAGATGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2358

Protein Analysis

221

Amino Acids

24.72

Weight (kDa)

6.83

Isoelectric Point (pI)

37.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2358 PF10184 84 - 196 1.1e-22 Uncharacterized conserved protein (DUF2358)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013501)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04890 AT3G04890 AT3G04890 AT3G04890
fragaria_vesca FvH4_6g03440
malus_domestica MD04G1220800.v1.1
prunus_persica Prupe.6G339800_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0451941
rosa_laevigata RLG00000025636
rosa_multiflora Rmu_sc0002901.1_g000001
rosa_roxburghii Rroxscaffold_164G00436170 Rroxscaffold_6G00429110
rosa_rugosa Rorug02G0637600
rosa_samantha Rh3AG039300 Rh3BG040600 Rh3CG039100 Rh3DG039800
rosa_wichuraiana Rw3G002940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 82
AccB7I CCANNNNNTGG 1 cut(s) 428
AccI GTMKAC 1 cut(s) 552
AccII CGCG 1 cut(s) 287
AciI CCGC 3 cut(s) 5, 119, 206
AclWI GGATC 2 cut(s) 341, 354
AcoI YGGCCR 1 cut(s) 625
AcuI CTGAAG 3 cut(s) 348, 483, 527
AfaI GTAC 3 cut(s) 30, 370, 547
AfiI CCNNNNNNNGG 1 cut(s) 428
AflIII ACRYGT 1 cut(s) 285
AgsI TTSAA 4 cut(s) 181, 365, 394, 617
AjiI CACGTC 1 cut(s) 312
AluBI AGCT 4 cut(s) 157, 272, 511, 597
AluI AGCT 4 cut(s) 157, 272, 511, 597
Alw21I GWGCWC 1 cut(s) 159
AlwI GGATC 2 cut(s) 341, 354
AoxI GGCC 2 cut(s) 521, 625
ApeKI GCWGC 2 cut(s) 15, 78
AspLEI GCGC 1 cut(s) 83
AspS9I GGNCC 1 cut(s) 498
AvaII GGWCC 1 cut(s) 498
AxyI CCTNAGG 1 cut(s) 161
BalI TGGCCA 1 cut(s) 627
BamHI GGATCC 1 cut(s) 346
BanII GRGCYC 1 cut(s) 159
BauI CACGAG 1 cut(s) 55
BbsI GAAGAC 1 cut(s) 165
Bbv12I GWGCWC 1 cut(s) 159
BbvI GCAGC 2 cut(s) 2, 65
BccI CCATC 2 cut(s) 430, 533
BfaI CTAG 2 cut(s) 266, 479
BisI GCNGC 2 cut(s) 16, 79
BlsI GCNGC 2 cut(s) 17, 80
Bme18I GGWCC 1 cut(s) 498
BmgBI CACGTC 1 cut(s) 312
BmgT120I GGNCC 1 cut(s) 498
BmiI GGNNCC 2 cut(s) 148, 348
BpiI GAAGAC 1 cut(s) 165
BplI GAGNNNNNCTC 2 cut(s) 100, 132
Bsa29I ATCGAT 1 cut(s) 425
BsaJI CCNNGG 1 cut(s) 515
BsaWI WCCGGW 2 cut(s) 91, 143
Bsc4I CCNNNNNNNGG 1 cut(s) 428
Bse1I ACTGG 1 cut(s) 73
Bse21I CCTNAGG 1 cut(s) 161
BseCI ATCGAT 1 cut(s) 425
BseDI CCNNGG 1 cut(s) 515
BseGI GGATG 2 cut(s) 214, 568
BseLI CCNNNNNNNGG 1 cut(s) 428
BseMII CTCAG 2 cut(s) 175, 582
BseNI ACTGG 1 cut(s) 73
BseRI GAGGAG 1 cut(s) 149
BseXI GCAGC 2 cut(s) 2, 65
Bsh1236I CGCG 1 cut(s) 287
BshFI GGCC 2 cut(s) 523, 627
BshVI ATCGAT 1 cut(s) 425
BsiHKAI GWGCWC 1 cut(s) 159
BsiSI CCGG 2 cut(s) 92, 144
BslFI GGGAC 1 cut(s) 236
BslI CCNNNNNNNGG 1 cut(s) 428
BsmFI GGGAC 1 cut(s) 236
BsnI GGCC 2 cut(s) 523, 627
Bsp1286I GDGCHC 1 cut(s) 159
Bsp143I GATC 2 cut(s) 346, 441
Bsp19I CCATGG 1 cut(s) 515
BspACI CCGC 3 cut(s) 5, 119, 206
BspANI GGCC 2 cut(s) 523, 627
BspCNI CTCAG 2 cut(s) 174, 583
BspDI ATCGAT 1 cut(s) 425
BspFNI CGCG 1 cut(s) 287
BspLI GGNNCC 2 cut(s) 148, 348
BspPI GGATC 2 cut(s) 341, 354
BsrI ACTGG 1 cut(s) 73
BssECI CCNNGG 1 cut(s) 515
BssMI GATC 2 cut(s) 346, 441
BssSI CACGAG 1 cut(s) 55
BssT1I CCWWGG 1 cut(s) 515
Bst2BI CACGAG 1 cut(s) 55
Bst4CI ACNGT 1 cut(s) 550
BstAPI GCANNNNNTGC 1 cut(s) 323
BstC8I GCNNGC 2 cut(s) 385, 588
BstDEI CTNAG 3 cut(s) 161, 494, 591
BstDSI CCRYGG 1 cut(s) 515
BstF5I GGATG 2 cut(s) 214, 568
BstFNI CGCG 1 cut(s) 287
BstHHI GCGC 1 cut(s) 83
BstKTI GATC 2 cut(s) 349, 444
BstMBI GATC 2 cut(s) 346, 441
BstMWI GCNNNNNNNGC 2 cut(s) 323, 617
BstNSI RCATGY 1 cut(s) 590
BstUI CGCG 1 cut(s) 287
BstV1I GCAGC 2 cut(s) 2, 65
BstV2I GAAGAC 1 cut(s) 165
BstX2I RGATCY 1 cut(s) 346
BstYI RGATCY 1 cut(s) 346
Bsu15I ATCGAT 1 cut(s) 425
Bsu36I CCTNAGG 1 cut(s) 161
BsuRI GGCC 2 cut(s) 523, 627
BsuTUI ATCGAT 1 cut(s) 425
BtgI CCRYGG 1 cut(s) 515
BtrI CACGTC 1 cut(s) 312
BtsCI GGATG 2 cut(s) 214, 568
BtsI GCAGTG 1 cut(s) 144
BtsIMutI CAGTG 2 cut(s) 80, 144
Cac8I GCNNGC 2 cut(s) 385, 588
CfoI GCGC 1 cut(s) 83
Cfr13I GGNCC 1 cut(s) 498
ClaI ATCGAT 1 cut(s) 425
Csp6I GTAC 3 cut(s) 29, 369, 546
CviAII CATG 3 cut(s) 486, 516, 587
CviJI RGCY 7 cut(s) 149, 157, 272, 511, 523, 597, 627
CviKI_1 RGCY 7 cut(s) 149, 157, 272, 511, 523, 597, 627
CviQI GTAC 3 cut(s) 29, 369, 546
DdeI CTNAG 3 cut(s) 161, 494, 591
DpnI GATC 2 cut(s) 348, 443
DpnII GATC 2 cut(s) 346, 441
DraI TTTAAA 1 cut(s) 574
EaeI YGGCCR 1 cut(s) 625
EciI GGCGGA 1 cut(s) 108
Ecl136II GAGCTC 1 cut(s) 157
Eco130I CCWWGG 1 cut(s) 515
Eco147I AGGCCT 1 cut(s) 523
Eco24I GRGCYC 1 cut(s) 159
Eco47I GGWCC 1 cut(s) 498
Eco53kI GAGCTC 1 cut(s) 157
Eco57I CTGAAG 3 cut(s) 348, 483, 527
Eco81I CCTNAGG 1 cut(s) 161
EcoICRI GAGCTC 1 cut(s) 157
EcoO109I RGGNCCY 1 cut(s) 498
EcoT14I CCWWGG 1 cut(s) 515
EcoT38I GRGCYC 1 cut(s) 159
ErhI CCWWGG 1 cut(s) 515
FaeI CATG 3 cut(s) 489, 519, 590
FaiI YATR 6 cut(s) 239, 324, 381, 487, 517, 588
FaqI GGGAC 1 cut(s) 236
FatI CATG 3 cut(s) 485, 515, 586
FblI GTMKAC 1 cut(s) 552
Fnu4HI GCNGC 2 cut(s) 16, 79
FokI GGATG 2 cut(s) 221, 575
FriOI GRGCYC 1 cut(s) 159
Fsp4HI GCNGC 2 cut(s) 16, 79
FspBI CTAG 2 cut(s) 266, 479
FspI TGCGCA 1 cut(s) 82
GlaI GCGC 1 cut(s) 82
GluI GCNGC 2 cut(s) 16, 79
HaeIII GGCC 2 cut(s) 523, 627
HapII CCGG 2 cut(s) 92, 144
HhaI GCGC 1 cut(s) 83
Hin1II CATG 3 cut(s) 489, 519, 590
Hin6I GCGC 1 cut(s) 81
HinP1I GCGC 1 cut(s) 81
HinfI GANTC 5 cut(s) 34, 60, 262, 416, 458
HpaII CCGG 2 cut(s) 92, 144
Hpy166II GTNNAC 1 cut(s) 553
Hpy188I TCNGA 4 cut(s) 24, 463, 638, 655
Hpy188III TCNNGA 3 cut(s) 266, 295, 445
Hpy8I GTNNAC 1 cut(s) 553
Hpy99I CGWCG 1 cut(s) 316
HpyAV CCTTC 3 cut(s) 187, 442, 651
HpyCH4III ACNGT 1 cut(s) 550
HpyCH4IV ACGT 1 cut(s) 311
HpyCH4V TGCA 1 cut(s) 611
HpyF10VI GCNNNNNNNGC 2 cut(s) 323, 617
HpyF3I CTNAG 3 cut(s) 161, 494, 591
HpySE526I ACGT 1 cut(s) 311
Hsp92II CATG 3 cut(s) 489, 519, 590
HspAI GCGC 1 cut(s) 81
Kzo9I GATC 2 cut(s) 346, 441
LmnI GCTCC 4 cut(s) 48, 146, 162, 516
Lsp1109I GCAGC 2 cut(s) 2, 65
MaeI CTAG 2 cut(s) 266, 479
MaeII ACGT 1 cut(s) 311
MaeIII GTNAC 2 cut(s) 139, 295
MalI GATC 2 cut(s) 348, 443
MboI GATC 2 cut(s) 346, 441
MboII GAAGA 2 cut(s) 165, 341
MfeI CAATTG 1 cut(s) 612
MflI RGATCY 1 cut(s) 346
MhlI GDGCHC 1 cut(s) 159
MlsI TGGCCA 1 cut(s) 627
MluCI AATT 4 cut(s) 318, 395, 576, 612
MluI ACGCGT 1 cut(s) 285
MluNI TGGCCA 1 cut(s) 627
MlyI GAGTC 1 cut(s) 410
MmeI TCCRAC 1 cut(s) 374
MnlI CCTC 4 cut(s) 48, 170, 337, 513
Mox20I TGGCCA 1 cut(s) 627
MscI TGGCCA 1 cut(s) 627
MseI TTAA 2 cut(s) 129, 573
Msp20I TGGCCA 1 cut(s) 627
MspI CCGG 2 cut(s) 92, 144
MunI CAATTG 1 cut(s) 612
MvnI CGCG 1 cut(s) 287
MwoI GCNNNNNNNGC 2 cut(s) 323, 617
NcoI CCATGG 1 cut(s) 515
NdeII GATC 2 cut(s) 346, 441
NlaIII CATG 3 cut(s) 489, 519, 590
NlaIV GGNNCC 2 cut(s) 148, 348
NmuCI GTSAC 2 cut(s) 139, 295
NsbI TGCGCA 1 cut(s) 82
NspI RCATGY 1 cut(s) 590
PaeI GCATGC 1 cut(s) 590
PceI AGGCCT 1 cut(s) 523
PcsI WCGNNNNNNNCGW 1 cut(s) 291
PfeI GAWTC 4 cut(s) 34, 60, 262, 458
PflMI CCANNNNNTGG 1 cut(s) 428
PkrI GCNGC 2 cut(s) 17, 80
PleI GAGTC 1 cut(s) 410
PpsI GAGTC 1 cut(s) 410
PpuMI RGGWCCY 1 cut(s) 498
Psp124BI GAGCTC 1 cut(s) 159
Psp5II RGGWCCY 1 cut(s) 498
PspN4I GGNNCC 2 cut(s) 148, 348
PspPI GGNCC 1 cut(s) 498
PspPPI RGGWCCY 1 cut(s) 498
PsuI RGATCY 1 cut(s) 346
RsaI GTAC 3 cut(s) 30, 370, 547
RsaNI GTAC 3 cut(s) 29, 369, 546
SacI GAGCTC 1 cut(s) 159
SaqAI TTAA 2 cut(s) 129, 573
SatI GCNGC 2 cut(s) 16, 79
Sau3AI GATC 2 cut(s) 346, 441
Sau96I GGNCC 1 cut(s) 498
SchI GAGTC 1 cut(s) 410
SduI GDGCHC 1 cut(s) 159
SinI GGWCC 1 cut(s) 498
SphI GCATGC 1 cut(s) 590
Sse9I AATT 4 cut(s) 318, 395, 576, 612
SseBI AGGCCT 1 cut(s) 523
SsiI CCGC 3 cut(s) 5, 119, 206
SspMI CTAG 2 cut(s) 266, 479
SstI GAGCTC 1 cut(s) 159
StuI AGGCCT 1 cut(s) 523
StyI CCWWGG 1 cut(s) 515
TaaI ACNGT 1 cut(s) 550
TaiI ACGT 1 cut(s) 314
TaqI TCGA 3 cut(s) 260, 425, 444
TasI AATT 4 cut(s) 318, 395, 576, 612
TfiI GAWTC 4 cut(s) 34, 60, 262, 458
Tru1I TTAA 2 cut(s) 129, 573
Tru9I TTAA 2 cut(s) 129, 573
TscAI CASTG 2 cut(s) 80, 144
TseFI GTSAC 2 cut(s) 139, 295
TseI GCWGC 2 cut(s) 15, 78
Tsp45I GTSAC 2 cut(s) 139, 295
TspGWI ACGGA 2 cut(s) 46, 386
TspRI CASTG 2 cut(s) 80, 144
Van91I CCANNNNNTGG 1 cut(s) 428
VpaK11BI GGWCC 1 cut(s) 498
XbaI TCTAGA 1 cut(s) 265
XceI RCATGY 1 cut(s) 590
XmiI GTMKAC 1 cut(s) 552
XspI CTAG 2 cut(s) 266, 479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.