RLG00000025636

Uncharacterized conserved protein (DUF2358)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0013501
Physical Location & Seq
Reverse (-)
47230818 .. 47233279
2462 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025636

Sequence Viewer

Length: 678 bp
ATGGCGGGAGTGTCCTGCTCTCTGAGACTACAGATTCCTCTGTTTTCACACCCCACGAGAATCCATGGAATCCGGTGCTGCGCAAAAGCACCCGATAACAATCCCAAGAGAGAAAAAACTCCGCAGATATTGAAACTCGCAGTGACCGGAGTCACTGAGTTACTGAGGCTTCTATCTCCGTTCAACACAACAACAACAACAACAACCAGATTGGATAAAGCGACTGATAAACAAAGGGATGAGTTTTCGGTTGTGGGAATAGATGATGTTGTGATGATTCTCGAAGCTGATTACAAAAATGCGTATTTTGTGACAGGCATTTTCACCTCCGCAATCTATGCTGAAGATTGTATCTTTGAAGATCCAACTATTAGATTTCAAGGTACGGAGTTGTATGCTCGCAACTTGAAATTGCTTGTTCCTTTCTTTGACTCTCCATCAATTGGATTGGAAAAGATTGAGAAGGGTATTGATTCTGAAGCAAACTTTGTTCTAGCAACATGGAAACTAAGGACCTACCTGAAGCTCCCATGGAGGCCTTTGATTTCTATTGATGGAAGTACAGTCTATGACTTGGATGATAAGTTTAAAATTGTAAGGCATGCTGAGAGTTGGAATGTTTCTGCAATTGAGGCAGTTGGCCAGATATTCAGACCTTCTGGAAGGTCCGATGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2358

Protein Analysis

226

Amino Acids

25.33

Weight (kDa)

6.33

Isoelectric Point (pI)

37.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2358 PF10184 89 - 201 6.9e-23 Uncharacterized conserved protein (DUF2358)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013501)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04890 AT3G04890 AT3G04890 AT3G04890
fragaria_vesca FvH4_6g03440
malus_domestica MD04G1220800.v1.1
prunus_persica Prupe.6G339800_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0451941
rosa_laevigata RLG00000025636
rosa_multiflora Rmu_sc0002901.1_g000001
rosa_roxburghii Rroxscaffold_164G00436170 Rroxscaffold_6G00429110
rosa_rugosa Rorug02G0637600
rosa_samantha Rh3AG039300 Rh3BG040600 Rh3CG039100 Rh3DG039800
rosa_wichuraiana Rw3G002940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 82
AccB7I CCANNNNNTGG 1 cut(s) 443
AciI CCGC 3 cut(s) 5, 122, 330
AclWI GGATC 1 cut(s) 356
AcoI YGGCCR 1 cut(s) 640
AcuI CTGAAG 3 cut(s) 363, 498, 542
AfaI GTAC 2 cut(s) 385, 562
AfiI CCNNNNNNNGG 1 cut(s) 443
AgsI TTSAA 5 cut(s) 133, 184, 359, 380, 409
AluBI AGCT 2 cut(s) 287, 526
AluI AGCT 2 cut(s) 287, 526
Alw26I GTCTC 1 cut(s) 19
AlwI GGATC 1 cut(s) 356
AoxI GGCC 2 cut(s) 536, 640
ApeKI GCWGC 1 cut(s) 78
AspLEI GCGC 1 cut(s) 83
AspS9I GGNCC 2 cut(s) 513, 666
AsuHPI GGTGA 1 cut(s) 316
AvaII GGWCC 2 cut(s) 513, 666
BalI TGGCCA 1 cut(s) 642
BauI CACGAG 1 cut(s) 55
BbvI GCAGC 1 cut(s) 65
BccI CCATC 2 cut(s) 445, 548
BcoDI GTCTC 1 cut(s) 19
BfaI CTAG 1 cut(s) 494
BfmI CTRYAG 1 cut(s) 29
BisI GCNGC 1 cut(s) 79
BlsI GCNGC 1 cut(s) 80
Bme18I GGWCC 2 cut(s) 513, 666
BmgT120I GGNCC 2 cut(s) 513, 666
BoxI GACNNNNGTC 1 cut(s) 149
BsaBI GATNNNNATC 1 cut(s) 99
BsaJI CCNNGG 2 cut(s) 64, 530
BsaWI WCCGGW 2 cut(s) 72, 146
Bsc4I CCNNNNNNNGG 1 cut(s) 443
Bse8I GATNNNNATC 1 cut(s) 99
BseDI CCNNGG 2 cut(s) 64, 530
BseGI GGATG 2 cut(s) 244, 583
BseJI GATNNNNATC 1 cut(s) 99
BseLI CCNNNNNNNGG 1 cut(s) 443
BseMII CTCAG 4 cut(s) 14, 147, 155, 597
BseXI GCAGC 1 cut(s) 65
BshFI GGCC 2 cut(s) 538, 642
BsiSI CCGG 2 cut(s) 73, 147
BslI CCNNNNNNNGG 1 cut(s) 443
BsmAI GTCTC 1 cut(s) 19
BsnI GGCC 2 cut(s) 538, 642
Bsp143I GATC 1 cut(s) 361
Bsp19I CCATGG 2 cut(s) 64, 530
BspACI CCGC 3 cut(s) 5, 122, 330
BspANI GGCC 2 cut(s) 538, 642
BspCNI CTCAG 4 cut(s) 15, 148, 156, 598
BspPI GGATC 1 cut(s) 356
BssECI CCNNGG 2 cut(s) 64, 530
BssMI GATC 1 cut(s) 361
BssSI CACGAG 1 cut(s) 55
BssT1I CCWWGG 2 cut(s) 64, 530
Bst2BI CACGAG 1 cut(s) 55
Bst4CI ACNGT 1 cut(s) 565
BstAPI GCANNNNNTGC 1 cut(s) 338
BstC8I GCNNGC 2 cut(s) 400, 603
BstDEI CTNAG 5 cut(s) 23, 156, 164, 509, 606
BstDSI CCRYGG 2 cut(s) 64, 530
BstF5I GGATG 2 cut(s) 244, 583
BstHHI GCGC 1 cut(s) 83
BstKTI GATC 1 cut(s) 364
BstMAI GTCTC 1 cut(s) 19
BstMBI GATC 1 cut(s) 361
BstMWI GCNNNNNNNGC 2 cut(s) 338, 632
BstNSI RCATGY 1 cut(s) 605
BstPAI GACNNNNGTC 1 cut(s) 149
BstSFI CTRYAG 1 cut(s) 29
BstV1I GCAGC 1 cut(s) 65
BstX2I RGATCY 1 cut(s) 361
BstYI RGATCY 1 cut(s) 361
BsuRI GGCC 2 cut(s) 538, 642
BtgI CCRYGG 2 cut(s) 64, 530
BtsCI GGATG 2 cut(s) 244, 583
BtsI GCAGTG 1 cut(s) 147
BtsIMutI CAGTG 2 cut(s) 147, 153
Cac8I GCNNGC 2 cut(s) 400, 603
CfoI GCGC 1 cut(s) 83
Cfr13I GGNCC 2 cut(s) 513, 666
Csp6I GTAC 2 cut(s) 384, 561
CviAII CATG 4 cut(s) 65, 501, 531, 602
CviJI RGCY 5 cut(s) 169, 287, 526, 538, 642
CviKI_1 RGCY 5 cut(s) 169, 287, 526, 538, 642
CviQI GTAC 2 cut(s) 384, 561
DdeI CTNAG 5 cut(s) 23, 156, 164, 509, 606
DpnI GATC 1 cut(s) 363
DpnII GATC 1 cut(s) 361
DraI TTTAAA 1 cut(s) 589
EaeI YGGCCR 1 cut(s) 640
Eco130I CCWWGG 2 cut(s) 64, 530
Eco147I AGGCCT 1 cut(s) 538
Eco47I GGWCC 2 cut(s) 513, 666
Eco57I CTGAAG 3 cut(s) 363, 498, 542
EcoO109I RGGNCCY 1 cut(s) 513
EcoT14I CCWWGG 2 cut(s) 64, 530
ErhI CCWWGG 2 cut(s) 64, 530
FaeI CATG 4 cut(s) 68, 504, 534, 605
FaiI YATR 7 cut(s) 66, 339, 396, 502, 532, 570, 603
FatI CATG 4 cut(s) 64, 500, 530, 601
Fnu4HI GCNGC 1 cut(s) 79
FokI GGATG 2 cut(s) 251, 590
Fsp4HI GCNGC 1 cut(s) 79
FspBI CTAG 1 cut(s) 494
FspI TGCGCA 1 cut(s) 82
GlaI GCGC 1 cut(s) 82
GluI GCNGC 1 cut(s) 79
HaeIII GGCC 2 cut(s) 538, 642
HapII CCGG 2 cut(s) 73, 147
HhaI GCGC 1 cut(s) 83
Hin1II CATG 4 cut(s) 68, 504, 534, 605
Hin6I GCGC 1 cut(s) 81
HinP1I GCGC 1 cut(s) 81
HinfI GANTC 7 cut(s) 34, 60, 69, 150, 277, 431, 473
HpaII CCGG 2 cut(s) 73, 147
HphI GGTGA 1 cut(s) 316
Hpy188I TCNGA 4 cut(s) 24, 478, 653, 670
Hpy188III TCNNGA 2 cut(s) 281, 660
HpyAV CCTTC 3 cut(s) 457, 657, 666
HpyCH4III ACNGT 1 cut(s) 565
HpyCH4V TGCA 1 cut(s) 626
HpyF10VI GCNNNNNNNGC 2 cut(s) 338, 632
HpyF3I CTNAG 5 cut(s) 23, 156, 164, 509, 606
Hsp92II CATG 4 cut(s) 68, 504, 534, 605
HspAI GCGC 1 cut(s) 81
Kzo9I GATC 1 cut(s) 361
LmnI GCTCC 1 cut(s) 531
LpnPI CCDG 8 cut(s) 28, 86, 160, 220, 300, 533, 645, 656
Lsp1109I GCAGC 1 cut(s) 65
MaeI CTAG 1 cut(s) 494
MaeIII GTNAC 4 cut(s) 142, 151, 159, 310
MalI GATC 1 cut(s) 363
MboI GATC 1 cut(s) 361
MboII GAAGA 2 cut(s) 356, 371
MfeI CAATTG 2 cut(s) 441, 627
MflI RGATCY 1 cut(s) 361
MlsI TGGCCA 1 cut(s) 642
MluCI AATT 4 cut(s) 410, 441, 591, 627
MluNI TGGCCA 1 cut(s) 642
MlyI GAGTC 2 cut(s) 159, 425
MmeI TCCRAC 2 cut(s) 389, 593
MnlI CCTC 5 cut(s) 48, 159, 337, 528, 625
Mox20I TGGCCA 1 cut(s) 642
MscI TGGCCA 1 cut(s) 642
MseI TTAA 1 cut(s) 588
Msp20I TGGCCA 1 cut(s) 642
MspI CCGG 2 cut(s) 73, 147
MunI CAATTG 2 cut(s) 441, 627
MwoI GCNNNNNNNGC 2 cut(s) 338, 632
NcoI CCATGG 2 cut(s) 64, 530
NdeII GATC 1 cut(s) 361
NlaIII CATG 4 cut(s) 68, 504, 534, 605
NmuCI GTSAC 3 cut(s) 142, 151, 310
NsbI TGCGCA 1 cut(s) 82
NspI RCATGY 1 cut(s) 605
PaeI GCATGC 1 cut(s) 605
PceI AGGCCT 1 cut(s) 538
PfeI GAWTC 5 cut(s) 34, 60, 69, 277, 473
PflMI CCANNNNNTGG 1 cut(s) 443
PkrI GCNGC 1 cut(s) 80
PleI GAGTC 2 cut(s) 158, 425
PpsI GAGTC 2 cut(s) 158, 425
PpuMI RGGWCCY 1 cut(s) 513
PshAI GACNNNNGTC 1 cut(s) 149
Psp5II RGGWCCY 1 cut(s) 513
PspPI GGNCC 2 cut(s) 513, 666
PspPPI RGGWCCY 1 cut(s) 513
PsuI RGATCY 1 cut(s) 361
RsaI GTAC 2 cut(s) 385, 562
RsaNI GTAC 2 cut(s) 384, 561
SaqAI TTAA 1 cut(s) 588
SatI GCNGC 1 cut(s) 79
Sau3AI GATC 1 cut(s) 361
Sau96I GGNCC 2 cut(s) 513, 666
SchI GAGTC 2 cut(s) 159, 425
SetI ASST 8 cut(s) 289, 329, 385, 518, 522, 528, 658, 668
SfcI CTRYAG 1 cut(s) 29
SinI GGWCC 2 cut(s) 513, 666
SphI GCATGC 1 cut(s) 605
Sse9I AATT 4 cut(s) 410, 441, 591, 627
SseBI AGGCCT 1 cut(s) 538
SsiI CCGC 3 cut(s) 5, 122, 330
SspMI CTAG 1 cut(s) 494
StuI AGGCCT 1 cut(s) 538
StyI CCWWGG 2 cut(s) 64, 530
TaaI ACNGT 1 cut(s) 565
TaqI TCGA 1 cut(s) 282
TasI AATT 4 cut(s) 410, 441, 591, 627
TatI WGTACW 1 cut(s) 560
TfiI GAWTC 5 cut(s) 34, 60, 69, 277, 473
Tru1I TTAA 1 cut(s) 588
Tru9I TTAA 1 cut(s) 588
TscAI CASTG 2 cut(s) 147, 160
TseFI GTSAC 3 cut(s) 142, 151, 310
TseI GCWGC 1 cut(s) 78
Tsp45I GTSAC 3 cut(s) 142, 151, 310
TspGWI ACGGA 2 cut(s) 168, 401
TspRI CASTG 2 cut(s) 147, 160
Van91I CCANNNNNTGG 1 cut(s) 443
VpaK11BI GGWCC 2 cut(s) 513, 666
XceI RCATGY 1 cut(s) 605
XspI CTAG 1 cut(s) 494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.