Rroxscaffold_164G00436170

Uncharacterized conserved protein (DUF2358)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000164
OG0013501
Physical Location & Seq
Reverse (-)
155251 .. 161493
6243 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_164G00436170.1

Sequence Viewer

Length: 720 bp
ATGGCGGGAGTGTGCTGCTCTCTGAGACTACATATTCCTCTGCTCTCACACCCCACGACGAACCATGGAATCAGGTGCTGCGCAAAAGCACCCGATAACAATCCCAAAAGAGAAACTCCGCGGATATTGAAACTCGCAGTGTCCGGAGTCACTGAGTTCCTCAGGCTTCTATCTTCGTTCAACACAACAAGAACCACCAGCCAGGGTTTGATTCAATTAATACCCTGCAGATTGGATAAAGTGACTGACAAACAAAGGGATGAGTTTTCGGATGTGGGAATAGATGATGTTGTAATGATTCTTGAAGCGGATTACAAGAATGCGTATTTTGTGACGGTGATAGTTTCCATTGGAATAGGCATTTTCACCTCTGCAATCTATGCTGAAGACTGTATCTTTGAAGATCCAACTATTAGATTTCAAGGTACCGAGTTGTATGCTCGCAACTTGAAATTGCTTGTTCCTTTCTTTGACTCTCCATCAATTGGATTGGAAAAGATCGAGAAGGGTATTGATTCTGAAGCAAACTTTGTTCTAGCAACATGGAAACTAAGGACCTACCTGAAGCTCCCATGGAGGCCTTTGATTTCTATTGATGGAAGTACAGTCTATGACTTGGATGATAAGTTTAAAGTTGTAAGGCATGCTGAGAGTTGGAATGTTTCTGCAATTGAGGCAGTTGGCCAGATATTCAGACCTTCTGGAAGGTCCGATGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2358

Protein Analysis

239

Amino Acids

26.8

Weight (kDa)

6.12

Isoelectric Point (pI)

31.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2358 PF10184 123 - 215 1.6e-19 Uncharacterized conserved protein (DUF2358)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013501)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04890 AT3G04890 AT3G04890 AT3G04890
fragaria_vesca FvH4_6g03440
malus_domestica MD04G1220800.v1.1
prunus_persica Prupe.6G339800_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0451941
rosa_laevigata RLG00000025636
rosa_multiflora Rmu_sc0002901.1_g000001
rosa_roxburghii Rroxscaffold_164G00436170 Rroxscaffold_6G00429110
rosa_rugosa Rorug02G0637600
rosa_samantha Rh3AG039300 Rh3BG040600 Rh3CG039100 Rh3DG039800
rosa_wichuraiana Rw3G002940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 82
Acc65I GGTACC 1 cut(s) 425
AccB1I GGYRCC 1 cut(s) 425
AccB7I CCANNNNNTGG 1 cut(s) 485
AccII CGCG 1 cut(s) 121
AccIII TCCGGA 1 cut(s) 143
AciI CCGC 4 cut(s) 5, 119, 121, 308
AclWI GGATC 1 cut(s) 398
AcoI YGGCCR 1 cut(s) 682
AcuI CTGAAG 3 cut(s) 405, 540, 584
AfaI GTAC 2 cut(s) 427, 604
AfiI CCNNNNNNNGG 1 cut(s) 485
AgsI TTSAA 7 cut(s) 130, 181, 215, 305, 401, 422, 451
AjnI CCWGG 1 cut(s) 201
AluBI AGCT 1 cut(s) 568
AluI AGCT 1 cut(s) 568
Alw26I GTCTC 1 cut(s) 19
AlwI GGATC 1 cut(s) 398
AlwNI CAGNNNCTG 1 cut(s) 78
Aor13HI TCCGGA 1 cut(s) 143
AoxI GGCC 2 cut(s) 578, 682
ApeKI GCWGC 2 cut(s) 15, 78
ArsI GACNNNNNNTTYG 2 cut(s) 380, 412
AseI ATTAAT 1 cut(s) 218
Asp718I GGTACC 1 cut(s) 425
AspLEI GCGC 1 cut(s) 83
AspS9I GGNCC 2 cut(s) 555, 708
AsuHPI GGTGA 2 cut(s) 349, 358
AvaII GGWCC 2 cut(s) 555, 708
AxyI CCTNAGG 1 cut(s) 161
BalI TGGCCA 1 cut(s) 684
BanI GGYRCC 1 cut(s) 425
BbsI GAAGAC 1 cut(s) 393
BbvI GCAGC 2 cut(s) 2, 65
BccI CCATC 2 cut(s) 487, 590
BcgI CGANNNNNNTGC 2 cut(s) 419, 453
BciT130I CCWGG 1 cut(s) 203
BcoDI GTCTC 1 cut(s) 19
BfaI CTAG 1 cut(s) 536
BfmI CTRYAG 1 cut(s) 226
BisI GCNGC 2 cut(s) 16, 79
BlsI GCNGC 2 cut(s) 17, 80
Bme1390I CCNGG 1 cut(s) 203
Bme18I GGWCC 2 cut(s) 555, 708
BmgT120I GGNCC 2 cut(s) 555, 708
BmiI GGNNCC 1 cut(s) 427
BmrFI CCNGG 1 cut(s) 203
BpiI GAAGAC 1 cut(s) 393
BsaBI GATNNNNATC 1 cut(s) 99
BsaJI CCNNGG 4 cut(s) 64, 119, 202, 572
BsaWI WCCGGW 1 cut(s) 143
Bsc4I CCNNNNNNNGG 1 cut(s) 485
Bse21I CCTNAGG 1 cut(s) 161
Bse8I GATNNNNATC 1 cut(s) 99
BseAI TCCGGA 1 cut(s) 143
BseBI CCWGG 1 cut(s) 203
BseDI CCNNGG 4 cut(s) 64, 119, 202, 572
BseGI GGATG 3 cut(s) 265, 277, 625
BseJI GATNNNNATC 1 cut(s) 99
BseLI CCNNNNNNNGG 1 cut(s) 485
BseMII CTCAG 4 cut(s) 14, 144, 175, 639
BseXI GCAGC 2 cut(s) 2, 65
Bsh1236I CGCG 1 cut(s) 121
BshFI GGCC 2 cut(s) 580, 684
BshNI GGYRCC 1 cut(s) 425
BsiSI CCGG 1 cut(s) 144
BslI CCNNNNNNNGG 1 cut(s) 485
BsmAI GTCTC 1 cut(s) 19
BsmI GAATGC 1 cut(s) 325
BsnI GGCC 2 cut(s) 580, 684
Bsp13I TCCGGA 1 cut(s) 143
Bsp143I GATC 2 cut(s) 403, 498
Bsp19I CCATGG 2 cut(s) 64, 572
BspACI CCGC 4 cut(s) 5, 119, 121, 308
BspANI GGCC 2 cut(s) 580, 684
BspCNI CTCAG 4 cut(s) 15, 145, 174, 640
BspEI TCCGGA 1 cut(s) 143
BspFNI CGCG 1 cut(s) 121
BspLI GGNNCC 1 cut(s) 427
BspMAI CTGCAG 1 cut(s) 230
BspPI GGATC 1 cut(s) 398
BspT107I GGYRCC 1 cut(s) 425
BssECI CCNNGG 4 cut(s) 64, 119, 202, 572
BssMI GATC 2 cut(s) 403, 498
BssT1I CCWWGG 2 cut(s) 64, 572
Bst2UI CCWGG 1 cut(s) 203
Bst4CI ACNGT 3 cut(s) 337, 392, 607
BstAPI GCANNNNNTGC 1 cut(s) 380
BstC8I GCNNGC 2 cut(s) 442, 645
BstDEI CTNAG 5 cut(s) 23, 153, 161, 551, 648
BstDSI CCRYGG 3 cut(s) 64, 119, 572
BstF5I GGATG 3 cut(s) 265, 277, 625
BstFNI CGCG 1 cut(s) 121
BstHHI GCGC 1 cut(s) 83
BstKTI GATC 2 cut(s) 406, 501
BstMAI GTCTC 1 cut(s) 19
BstMBI GATC 2 cut(s) 403, 498
BstMWI GCNNNNNNNGC 2 cut(s) 380, 674
BstNI CCWGG 1 cut(s) 203
BstNSI RCATGY 1 cut(s) 647
BstSCI CCNGG 1 cut(s) 201
BstSFI CTRYAG 1 cut(s) 226
BstUI CGCG 1 cut(s) 121
BstV1I GCAGC 2 cut(s) 2, 65
BstV2I GAAGAC 1 cut(s) 393
BstX2I RGATCY 1 cut(s) 403
BstYI RGATCY 1 cut(s) 403
Bsu36I CCTNAGG 1 cut(s) 161
BsuRI GGCC 2 cut(s) 580, 684
BtgI CCRYGG 3 cut(s) 64, 119, 572
BtsCI GGATG 3 cut(s) 265, 277, 625
BtsI GCAGTG 1 cut(s) 144
BtsIMutI CAGTG 2 cut(s) 144, 150
Cac8I GCNNGC 2 cut(s) 442, 645
CaiI CAGNNNCTG 1 cut(s) 78
CfoI GCGC 1 cut(s) 83
Cfr13I GGNCC 2 cut(s) 555, 708
Cfr42I CCGCGG 1 cut(s) 122
Csp6I GTAC 2 cut(s) 426, 603
CviAII CATG 4 cut(s) 65, 543, 573, 644
CviJI RGCY 5 cut(s) 166, 201, 568, 580, 684
CviKI_1 RGCY 5 cut(s) 166, 201, 568, 580, 684
CviQI GTAC 2 cut(s) 426, 603
DdeI CTNAG 5 cut(s) 23, 153, 161, 551, 648
DpnI GATC 2 cut(s) 405, 500
DpnII GATC 2 cut(s) 403, 498
DraI TTTAAA 1 cut(s) 631
EaeI YGGCCR 1 cut(s) 682
Eco130I CCWWGG 2 cut(s) 64, 572
Eco147I AGGCCT 1 cut(s) 580
Eco47I GGWCC 2 cut(s) 555, 708
Eco57I CTGAAG 3 cut(s) 405, 540, 584
Eco81I CCTNAGG 1 cut(s) 161
EcoO109I RGGNCCY 1 cut(s) 555
EcoRII CCWGG 1 cut(s) 201
EcoT14I CCWWGG 2 cut(s) 64, 572
ErhI CCWWGG 2 cut(s) 64, 572
FaeI CATG 4 cut(s) 68, 546, 576, 647
FaiI YATR 8 cut(s) 33, 66, 381, 438, 544, 574, 612, 645
FatI CATG 4 cut(s) 64, 542, 572, 643
Fnu4HI GCNGC 2 cut(s) 16, 79
FokI GGATG 3 cut(s) 272, 284, 632
Fsp4HI GCNGC 2 cut(s) 16, 79
FspBI CTAG 1 cut(s) 536
FspI TGCGCA 1 cut(s) 82
GlaI GCGC 1 cut(s) 82
GluI GCNGC 2 cut(s) 16, 79
HaeIII GGCC 2 cut(s) 580, 684
HapII CCGG 1 cut(s) 144
HhaI GCGC 1 cut(s) 83
Hin1II CATG 4 cut(s) 68, 546, 576, 647
Hin6I GCGC 1 cut(s) 81
HinP1I GCGC 1 cut(s) 81
HinfI GANTC 6 cut(s) 69, 147, 211, 298, 473, 515
HpaII CCGG 1 cut(s) 144
HphI GGTGA 2 cut(s) 349, 358
Hpy188I TCNGA 5 cut(s) 24, 271, 520, 695, 712
Hpy188III TCNNGA 4 cut(s) 144, 302, 502, 702
Hpy99I CGWCG 1 cut(s) 61
HpyAV CCTTC 3 cut(s) 499, 699, 708
HpyCH4III ACNGT 3 cut(s) 337, 392, 607
HpyCH4V TGCA 3 cut(s) 228, 374, 668
HpyF10VI GCNNNNNNNGC 2 cut(s) 380, 674
HpyF3I CTNAG 5 cut(s) 23, 153, 161, 551, 648
Hsp92II CATG 4 cut(s) 68, 546, 576, 647
HspAI GCGC 1 cut(s) 81
Kpn2I TCCGGA 1 cut(s) 143
KpnI GGTACC 1 cut(s) 429
KspI CCGCGG 1 cut(s) 122
Kzo9I GATC 2 cut(s) 403, 498
LmnI GCTCC 1 cut(s) 573
Lsp1109I GCAGC 2 cut(s) 2, 65
MaeI CTAG 1 cut(s) 536
MaeIII GTNAC 3 cut(s) 148, 241, 331
MalI GATC 2 cut(s) 405, 500
MboI GATC 2 cut(s) 403, 498
MboII GAAGA 3 cut(s) 165, 398, 413
MfeI CAATTG 2 cut(s) 483, 669
MflI RGATCY 1 cut(s) 403
MlsI TGGCCA 1 cut(s) 684
MluCI AATT 4 cut(s) 215, 452, 483, 669
MluNI TGGCCA 1 cut(s) 684
MlyI GAGTC 2 cut(s) 156, 467
MmeI TCCRAC 2 cut(s) 431, 635
MnlI CCTC 5 cut(s) 48, 170, 379, 570, 667
Mox20I TGGCCA 1 cut(s) 684
MroI TCCGGA 1 cut(s) 143
MscI TGGCCA 1 cut(s) 684
MseI TTAA 2 cut(s) 218, 630
Msp20I TGGCCA 1 cut(s) 684
MspA1I CMGCKG 1 cut(s) 121
MspI CCGG 1 cut(s) 144
MspR9I CCNGG 1 cut(s) 203
MunI CAATTG 2 cut(s) 483, 669
Mva1269I GAATGC 1 cut(s) 325
MvaI CCWGG 1 cut(s) 203
MvnI CGCG 1 cut(s) 121
MwoI GCNNNNNNNGC 2 cut(s) 380, 674
NcoI CCATGG 2 cut(s) 64, 572
NdeII GATC 2 cut(s) 403, 498
NlaIII CATG 4 cut(s) 68, 546, 576, 647
NlaIV GGNNCC 1 cut(s) 427
NmuCI GTSAC 3 cut(s) 148, 241, 331
NsbI TGCGCA 1 cut(s) 82
NspI RCATGY 1 cut(s) 647
PaeI GCATGC 1 cut(s) 647
PceI AGGCCT 1 cut(s) 580
PctI GAATGC 1 cut(s) 325
PfeI GAWTC 4 cut(s) 69, 211, 298, 515
PflMI CCANNNNNTGG 1 cut(s) 485
PkrI GCNGC 2 cut(s) 17, 80
PleI GAGTC 2 cut(s) 155, 467
PpsI GAGTC 2 cut(s) 155, 467
PpuMI RGGWCCY 1 cut(s) 555
PshBI ATTAAT 1 cut(s) 218
Psp5II RGGWCCY 1 cut(s) 555
Psp6I CCWGG 1 cut(s) 201
PspGI CCWGG 1 cut(s) 201
PspN4I GGNNCC 1 cut(s) 427
PspPI GGNCC 2 cut(s) 555, 708
PspPPI RGGWCCY 1 cut(s) 555
PstI CTGCAG 1 cut(s) 230
PstNI CAGNNNCTG 1 cut(s) 78
PsuI RGATCY 1 cut(s) 403
RsaI GTAC 2 cut(s) 427, 604
RsaNI GTAC 2 cut(s) 426, 603
SacII CCGCGG 1 cut(s) 122
SaqAI TTAA 2 cut(s) 218, 630
SatI GCNGC 2 cut(s) 16, 79
Sau3AI GATC 2 cut(s) 403, 498
Sau96I GGNCC 2 cut(s) 555, 708
SchI GAGTC 2 cut(s) 156, 467
ScrFI CCNGG 1 cut(s) 203
SetI ASST 8 cut(s) 77, 371, 427, 560, 564, 570, 700, 710
SfcI CTRYAG 1 cut(s) 226
Sfr303I CCGCGG 1 cut(s) 122
SgrBI CCGCGG 1 cut(s) 122
SinI GGWCC 2 cut(s) 555, 708
SphI GCATGC 1 cut(s) 647
Sse9I AATT 4 cut(s) 215, 452, 483, 669
SseBI AGGCCT 1 cut(s) 580
SsiI CCGC 4 cut(s) 5, 119, 121, 308
SspMI CTAG 1 cut(s) 536
StuI AGGCCT 1 cut(s) 580
StyD4I CCNGG 1 cut(s) 201
StyI CCWWGG 2 cut(s) 64, 572
TaaI ACNGT 3 cut(s) 337, 392, 607
TaqI TCGA 1 cut(s) 501
TasI AATT 4 cut(s) 215, 452, 483, 669
TatI WGTACW 1 cut(s) 602
TfiI GAWTC 4 cut(s) 69, 211, 298, 515
Tru1I TTAA 2 cut(s) 218, 630
Tru9I TTAA 2 cut(s) 218, 630
TscAI CASTG 2 cut(s) 144, 157
TseFI GTSAC 3 cut(s) 148, 241, 331
TseI GCWGC 2 cut(s) 15, 78
Tsp45I GTSAC 3 cut(s) 148, 241, 331
TspRI CASTG 2 cut(s) 144, 157
Van91I CCANNNNNTGG 1 cut(s) 485
VpaK11BI GGWCC 2 cut(s) 555, 708
VspI ATTAAT 1 cut(s) 218
XceI RCATGY 1 cut(s) 647
XspI CTAG 1 cut(s) 536
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.