MD04G1220800.v1.1

Uncharacterized conserved protein (DUF2358)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0013501
Physical Location & Seq
Reverse (-)
30188085 .. 30190619
2535 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1220800.v1.1.491

Sequence Viewer

Length: 669 bp
ATGGCGGGAGTAGCGTGTTACGGTTGCACTGCAACTGCAACTCTACGGCTTCCTCCGCTCCCGCAGTCGAAGGGAATCCGCGGCATCCGGTGCTCCTCCGCCGCACCGGATAACAACAGCAACAAGAGAGAAACCCCGCAGGTTTTGAAAATCGCTGTGAGCGGAGTCACTGAGCTGCTCAGGCTCCTCTCCTCCTCCAACAAAGCATCCAGATTAAATGGAGTGAGTGACAAAGAAAGCGATGAGTTTTCAGTTTCCGGCATTGATGATGTTGTAACGATTCTCCAATCTGATTTCGAGAATGCGTATTTTGTAACAGGGATTTTCACTTCTGCAATTTATACTGAAGATTGTGTCTTTGAAGATCCAACTATTAGATTTCGAGGTAAGGACTTGTATTCTCGCAACTTGAAATTGCTTGTTCCTTTCTTTGACTCTCCGTCAATTGGATTAGAAAAGATCGAGAAGGGTATCGATTCTGAAGCAAATTTTGTACTTGCAACATGGAAGCTCAGGACATACCTAAAACTTCCATGGAGGCCTCTCATTTGCATCGATGGAAGTACGGTCTATGACTTAGACGATAAGTTTAAAATTGTAAGGCACGCTGAAAGTTGGAATGTCTCTGCAATTGAGGCAATTGGCCAGCTGTTCACGCCTACTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2358

Protein Analysis

223

Amino Acids

24.51

Weight (kDa)

5.56

Isoelectric Point (pI)

42.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2358 PF10184 90 - 202 1.9e-22 Uncharacterized conserved protein (DUF2358)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013501)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G04890 AT3G04890 AT3G04890 AT3G04890
fragaria_vesca FvH4_6g03440
malus_domestica MD04G1220800.v1.1
prunus_persica Prupe.6G339800_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0451941
rosa_laevigata RLG00000025636
rosa_multiflora Rmu_sc0002901.1_g000001
rosa_roxburghii Rroxscaffold_164G00436170 Rroxscaffold_6G00429110
rosa_rugosa Rorug02G0637600
rosa_samantha Rh3AG039300 Rh3BG040600 Rh3CG039100 Rh3DG039800
rosa_wichuraiana Rw3G002940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 130
AccBSI CCGCTC 2 cut(s) 58, 162
AccII CGCG 1 cut(s) 81
AciI CCGC 9 cut(s) 5, 56, 62, 79, 81, 99, 102, 137, 162
AclWI GGATC 1 cut(s) 359
AcoI YGGCCR 1 cut(s) 643
AcsI RAATTY 1 cut(s) 487
AcuI CTGAAG 2 cut(s) 366, 501
AfaI GTAC 2 cut(s) 495, 565
AfiI CCNNNNNNNGG 1 cut(s) 446
AgsI TTSAA 3 cut(s) 148, 362, 412
AhdI GACNNNNNGTC 1 cut(s) 439
AluBI AGCT 3 cut(s) 175, 511, 649
AluI AGCT 3 cut(s) 175, 511, 649
Alw21I GWGCWC 1 cut(s) 95
Alw26I GTCTC 1 cut(s) 628
AlwI GGATC 1 cut(s) 359
AoxI GGCC 2 cut(s) 539, 643
ApeKI GCWGC 1 cut(s) 175
ApoI RAATTY 1 cut(s) 487
BalI TGGCCA 1 cut(s) 645
Bbv12I GWGCWC 1 cut(s) 95
BbvI GCAGC 1 cut(s) 162
BccI CCATC 1 cut(s) 551
BceAI ACGGC 1 cut(s) 62
BcoDI GTCTC 1 cut(s) 628
BfuAI ACCTGC 1 cut(s) 130
BisI GCNGC 3 cut(s) 82, 102, 176
BlsI GCNGC 3 cut(s) 83, 103, 177
BmeRI GACNNNNNGTC 1 cut(s) 439
BmiI GGNNCC 1 cut(s) 185
BmsI GCATC 3 cut(s) 93, 215, 561
Bpu10I CCTNAGC 2 cut(s) 179, 512
Bsa29I ATCGAT 2 cut(s) 474, 555
BsaJI CCNNGG 2 cut(s) 79, 533
BsaWI WCCGGW 2 cut(s) 87, 106
Bsc4I CCNNNNNNNGG 1 cut(s) 446
BseCI ATCGAT 2 cut(s) 474, 555
BseDI CCNNGG 2 cut(s) 79, 533
BseGI GGATG 2 cut(s) 84, 206
BseLI CCNNNNNNNGG 1 cut(s) 446
BseMII CTCAG 3 cut(s) 162, 193, 526
BseRI GAGGAG 4 cut(s) 85, 176, 181, 184
BseXI GCAGC 1 cut(s) 162
Bsh1236I CGCG 1 cut(s) 81
BshFI GGCC 2 cut(s) 541, 645
BshVI ATCGAT 2 cut(s) 474, 555
BsiHKAI GWGCWC 1 cut(s) 95
BsiSI CCGG 3 cut(s) 88, 107, 258
BslI CCNNNNNNNGG 1 cut(s) 446
BsmAI GTCTC 1 cut(s) 628
BsmI GAATGC 1 cut(s) 307
BsnI GGCC 2 cut(s) 541, 645
Bsp1286I GDGCHC 1 cut(s) 95
Bsp143I GATC 2 cut(s) 364, 459
Bsp19I CCATGG 1 cut(s) 533
BspACI CCGC 9 cut(s) 5, 56, 62, 79, 81, 99, 102, 137, 162
BspANI GGCC 2 cut(s) 541, 645
BspCNI CTCAG 3 cut(s) 163, 192, 525
BspDI ATCGAT 2 cut(s) 474, 555
BspFNI CGCG 1 cut(s) 81
BspLI GGNNCC 1 cut(s) 185
BspMI ACCTGC 1 cut(s) 130
BspPI GGATC 1 cut(s) 359
BsrBI CCGCTC 2 cut(s) 58, 162
BssECI CCNNGG 2 cut(s) 79, 533
BssMI GATC 2 cut(s) 364, 459
BssT1I CCWWGG 1 cut(s) 533
Bst4CI ACNGT 2 cut(s) 23, 568
BstAPI GCANNNNNTGC 1 cut(s) 90
BstC8I GCNNGC 2 cut(s) 606, 647
BstDEI CTNAG 4 cut(s) 171, 179, 512, 577
BstDSI CCRYGG 2 cut(s) 79, 533
BstF5I GGATG 2 cut(s) 84, 206
BstFNI CGCG 1 cut(s) 81
BstKTI GATC 2 cut(s) 367, 462
BstMAI GTCTC 1 cut(s) 628
BstMBI GATC 2 cut(s) 364, 459
BstMWI GCNNNNNNNGC 6 cut(s) 11, 55, 90, 181, 635, 655
BstUI CGCG 1 cut(s) 81
BstV1I GCAGC 1 cut(s) 162
BstX2I RGATCY 1 cut(s) 364
BstYI RGATCY 1 cut(s) 364
Bsu15I ATCGAT 2 cut(s) 474, 555
BsuRI GGCC 2 cut(s) 541, 645
BsuTUI ATCGAT 2 cut(s) 474, 555
BtgI CCRYGG 2 cut(s) 79, 533
BtgZI GCGATG 1 cut(s) 255
BtsCI GGATG 2 cut(s) 84, 206
BtsI GCAGTG 1 cut(s) 27
BtsIMutI CAGTG 2 cut(s) 27, 168
BveI ACCTGC 1 cut(s) 130
Cac8I GCNNGC 2 cut(s) 606, 647
Cfr42I CCGCGG 1 cut(s) 82
ClaI ATCGAT 2 cut(s) 474, 555
Csp6I GTAC 2 cut(s) 494, 564
CviAII CATG 2 cut(s) 504, 534
CviJI RGCY 7 cut(s) 49, 175, 184, 511, 541, 645, 649
CviKI_1 RGCY 7 cut(s) 49, 175, 184, 511, 541, 645, 649
CviQI GTAC 2 cut(s) 494, 564
DdeI CTNAG 4 cut(s) 171, 179, 512, 577
DpnI GATC 2 cut(s) 366, 461
DpnII GATC 2 cut(s) 364, 459
DraI TTTAAA 1 cut(s) 592
DriI GACNNNNNGTC 1 cut(s) 439
EaeI YGGCCR 1 cut(s) 643
Eam1105I GACNNNNNGTC 1 cut(s) 439
EciI GGCGGA 1 cut(s) 88
Eco130I CCWWGG 1 cut(s) 533
Eco147I AGGCCT 1 cut(s) 541
Eco57I CTGAAG 2 cut(s) 366, 501
EcoT14I CCWWGG 1 cut(s) 533
ErhI CCWWGG 1 cut(s) 533
FaeI CATG 2 cut(s) 507, 537
FaiI YATR 5 cut(s) 342, 505, 520, 535, 573
FatI CATG 2 cut(s) 503, 533
FauI CCCGC 2 cut(s) 69, 144
Fnu4HI GCNGC 3 cut(s) 82, 102, 176
FokI GGATG 2 cut(s) 71, 193
Fsp4HI GCNGC 3 cut(s) 82, 102, 176
GluI GCNGC 3 cut(s) 82, 102, 176
HaeIII GGCC 2 cut(s) 541, 645
HapII CCGG 3 cut(s) 88, 107, 258
Hin1II CATG 2 cut(s) 507, 537
HinfI GANTC 5 cut(s) 75, 165, 280, 434, 476
HpaII CCGG 3 cut(s) 88, 107, 258
Hpy166II GTNNAC 1 cut(s) 654
Hpy188I TCNGA 2 cut(s) 292, 481
Hpy188III TCNNGA 4 cut(s) 210, 298, 463, 514
Hpy8I GTNNAC 1 cut(s) 654
HpyAV CCTTC 2 cut(s) 64, 460
HpyCH4III ACNGT 2 cut(s) 23, 568
HpyCH4V TGCA 7 cut(s) 27, 32, 38, 335, 500, 552, 629
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 55, 90, 181, 635, 655
HpyF3I CTNAG 4 cut(s) 171, 179, 512, 577
Hsp92II CATG 2 cut(s) 507, 537
KspI CCGCGG 1 cut(s) 82
Kzo9I GATC 2 cut(s) 364, 459
LmnI GCTCC 3 cut(s) 63, 98, 189
LpnPI CCDG 9 cut(s) 101, 120, 125, 166, 223, 271, 303, 499, 659
Lsp1109I GCAGC 1 cut(s) 162
LweI GCATC 3 cut(s) 93, 215, 561
MaeIII GTNAC 5 cut(s) 17, 166, 227, 274, 313
MalI GATC 2 cut(s) 366, 461
MbiI CCGCTC 2 cut(s) 58, 162
MboI GATC 2 cut(s) 364, 459
MboII GAAGA 2 cut(s) 359, 374
MfeI CAATTG 3 cut(s) 444, 630, 639
MflI RGATCY 1 cut(s) 364
MhlI GDGCHC 1 cut(s) 95
MlsI TGGCCA 1 cut(s) 645
MluCI AATT 7 cut(s) 336, 413, 444, 487, 594, 630, 639
MluNI TGGCCA 1 cut(s) 645
MlyI GAGTC 2 cut(s) 174, 428
MmeI TCCRAC 3 cut(s) 222, 392, 596
MnlI CCTC 9 cut(s) 63, 106, 197, 202, 205, 377, 531, 552, 628
Mox20I TGGCCA 1 cut(s) 645
MscI TGGCCA 1 cut(s) 645
MseI TTAA 2 cut(s) 215, 591
Msp20I TGGCCA 1 cut(s) 645
MspA1I CMGCKG 2 cut(s) 81, 649
MspI CCGG 3 cut(s) 88, 107, 258
MunI CAATTG 3 cut(s) 444, 630, 639
Mva1269I GAATGC 1 cut(s) 307
MvnI CGCG 1 cut(s) 81
MwoI GCNNNNNNNGC 6 cut(s) 11, 55, 90, 181, 635, 655
NcoI CCATGG 1 cut(s) 533
NdeII GATC 2 cut(s) 364, 459
NlaIII CATG 2 cut(s) 507, 537
NlaIV GGNNCC 1 cut(s) 185
NmuCI GTSAC 2 cut(s) 166, 227
PceI AGGCCT 1 cut(s) 541
PctI GAATGC 1 cut(s) 307
PfeI GAWTC 3 cut(s) 75, 280, 476
PkrI GCNGC 3 cut(s) 83, 103, 177
PleI GAGTC 2 cut(s) 173, 428
PpsI GAGTC 2 cut(s) 173, 428
PspN4I GGNNCC 1 cut(s) 185
PsuI RGATCY 1 cut(s) 364
PvuII CAGCTG 1 cut(s) 649
RsaI GTAC 2 cut(s) 495, 565
RsaNI GTAC 2 cut(s) 494, 564
SacII CCGCGG 1 cut(s) 82
SaqAI TTAA 2 cut(s) 215, 591
SatI GCNGC 3 cut(s) 82, 102, 176
Sau3AI GATC 2 cut(s) 364, 459
SchI GAGTC 2 cut(s) 174, 428
SduI GDGCHC 1 cut(s) 95
SetI ASST 6 cut(s) 144, 177, 388, 513, 525, 651
SfaNI GCATC 3 cut(s) 93, 215, 561
Sfr303I CCGCGG 1 cut(s) 82
SgrBI CCGCGG 1 cut(s) 82
Sse9I AATT 7 cut(s) 336, 413, 444, 487, 594, 630, 639
SseBI AGGCCT 1 cut(s) 541
SsiI CCGC 9 cut(s) 5, 56, 62, 79, 81, 99, 102, 137, 162
StuI AGGCCT 1 cut(s) 541
StyI CCWWGG 1 cut(s) 533
TaaI ACNGT 2 cut(s) 23, 568
TaqI TCGA 6 cut(s) 68, 297, 382, 462, 474, 555
TasI AATT 7 cut(s) 336, 413, 444, 487, 594, 630, 639
TatI WGTACW 1 cut(s) 493
TauI GCSGC 2 cut(s) 84, 104
TfiI GAWTC 3 cut(s) 75, 280, 476
Tru1I TTAA 2 cut(s) 215, 591
Tru9I TTAA 2 cut(s) 215, 591
TscAI CASTG 2 cut(s) 34, 175
TseFI GTSAC 2 cut(s) 166, 227
TseI GCWGC 1 cut(s) 175
Tsp45I GTSAC 2 cut(s) 166, 227
TspGWI ACGGA 1 cut(s) 429
TspRI CASTG 2 cut(s) 34, 175
XapI RAATTY 1 cut(s) 487
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.