FvH4_6g07430

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0008008
Physical Location & Seq
Forward (+)
4453246 .. 4455507
2262 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g07430.t1

Sequence Viewer

Length: 1779 bp
ATGCATCCCAGGCTTCAAATCCTGAGGCCTCTCTGCTCTTTGGCTTGCGTACCCGGAAGACCCACATCGGAAAAATTGCATCTTGCGGTGGTTTCAGACCTTTTAGCAAAGCAGAGGTGGTCAGAGGTCAGAACCCATCTCAAAGATTTGAACTTTGGCTCAGTTCTTCATCAGGTTTGGGATTCTGGAGCAGACCCAGTACTTATTCTCAGGTACTTAAATAGTTCTCACAAGGATTTCAATGATGAGTATGATCTAGGACTCACTTTTGGGCTCTTAGGTTCATTAGCAGAAGCCAAAAGGTACTCAAAGATTCGAGCTTTTTTACATGAGTTTGTTAGAAGCAATGAGAACCTTTCAAATTCTGCTATTTTTAGATCGCTTTCATTGGGTAGGAGCCATTTTGGTGCCAATTCGGTTGTTGTTAACTTGTTGGTATTGGCATATGCGAAGAATTTGAAGACCCAGTTGGGTGTTGAAGCGTTTAGGAAAGCTGGTGAGTATGGGTTTAGGATGACCGTGTTTGCTTGTAACCCTTTGTTGAGGGCTTTGGTGAAGGAAGGTGAGGTTGGGAGTGTTGAATATGTTTACAGGGAGATGATTAGGAGGAGAATTAAGCCGAATTTGTATACTTTTAATATGGTGATCAGGGGGTTGTGTAAAGTTGGGAAGTTGGAGGAGGCGAAGGATGTTGTTGTTAATGATATGAAGGCTTGGGGGATATTGCCGAATGTGGTTAGTTATAATACTTTGATTGATGGGTATTGCAAATTTGGTGGGTTGGGGAGGATGTACAAAGCCGATGCGATTTTGAAGGAGATGAAGGAAAATAGTATTCAGCCGAATAAGTGTACTTACAATATTTTGATTAGTGGTTTTGGTAAAGATGAGAATATACCAGCTGCAATCAAAGTTTTTGAGGCGATAGTGAAGCAGGAGTTGAAACCCGATGTGTTTACATGTTGTTCGCTGATTAATGGGTTGTGCTGCATTGGGGAGCTTGACAAGGCTTGTGATTTGCTGGAGGAAATGTGTGGCTTGGGTTTGAAGCCAAATGTTATTATCTATAATGCTCTTATTAATGGGTTTTGTAAGAAGAAGATGATGAAGAGAGCTAGGGAGTTGTTTGATGACATTGGTAGACAAGGCTTGGAGCCGAGTGTTATAACGTACAATACATTAATTGATGCTTACTCTAAGGATGGGATGATTGAGGAAGCATTTGAGTTTTATAATTCAATGTTAGAGAGAGGAGTTTCTCCAACCACTTCGACCTTCAACTGCCGGATTTCTGGTTTGTGTCGACAAGGGAAAATGGAGATGGCATTGAAGCTTTTACATGAAATGGAGGGTAATGGATTGAGAGCTGATCGCATAACATATAACATATTTATAGATGGATACTGTAAAGAAGGGGATTCAAGAAAAGCACAGGAACTCTTCAATGAGATGATCAAGAAGCGTTTGAGTCCAAATCACTTGACATACAATTTTTTGATGGATGGCTACTGCAAAGAAGGAAACCTCAGTGCAGCTTTGAATCTGAGGGCACGGATGGAGAAGGGAGGGAAGCGACCAAATGTTGCAACATATAATTTGTTGATCAAGGGATATGCAAAGAAGGATAAACTTGAAGATGCAAATGGGCTTCTTAATGAGATGTTAGAGAAGGGTTTGGTCCCAAACCAAACTACTTATGAGATAGTAAAAGAGGAAATGATGAAGAAAGGTTTTGTTCCTCATGTAGATTGGTACCTGTATGATATCTCTAGCTCTTAG

Protein Analysis

593

Amino Acids

66.91

Weight (kDa)

8.81

Isoelectric Point (pI)

34.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_3 PF13812 163 - 217 3.9e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 173 - 221 3e-12 PPR repeat family
PPR_1 PF12854 204 - 232 1.8e-08 PPR repeat
PPR_2 PF13041 211 - 257 1.2e-14 PPR repeat family
PPR_3 PF13812 232 - 295 8e-13 Pentatricopeptide repeat domain
PPR_1 PF12854 240 - 259 4.5e-08 PPR repeat
PPR_2 PF13041 262 - 295 4.2e-08 PPR repeat family
PPR_1 PF12854 279 - 307 3.9e-06 PPR repeat
PPR_2 PF13041 284 - 329 2e-11 PPR repeat family
PPR_3 PF13812 304 - 363 5.3e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 313 - 344 4.6e-09 PPR repeat
PPR_1 PF12854 348 - 378 9.4e-11 PPR repeat
PPR_2 PF13041 351 - 397 1.4e-15 PPR repeat family
PPR PF01535 355 - 383 2.6e-06 PPR repeat
TPR_24 PF23276 374 - 483 6.4e-09 Fungal tetratrico peptide repeats
PPR_3 PF13812 375 - 428 6.3e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 383 - 414 1.3e-10 PPR repeat
PPR_2 PF13041 386 - 434 2.9e-17 PPR repeat family
PPR PF01535 389 - 419 3.1e-10 PPR repeat
PPR_1 PF12854 417 - 450 6.1e-09 PPR repeat
PPR_2 PF13041 421 - 470 4.1e-16 PPR repeat family
PPR PF01535 424 - 454 1.5e-07 PPR repeat
PPR_3 PF13812 444 - 503 2e-12 Pentatricopeptide repeat domain
PPR_1 PF12854 452 - 484 7.4e-12 PPR repeat
PPR_2 PF13041 457 - 505 2.4e-18 PPR repeat family
PPR PF01535 459 - 488 1.3e-09 PPR repeat
PPR_1 PF12854 488 - 520 2.5e-08 PPR repeat
PPR_2 PF13041 495 - 540 5.5e-14 PPR repeat family
PPR_3 PF13812 519 - 567 1.6e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 525 - 554 2.7e-07 PPR repeat
PPR_2 PF13041 526 - 569 2e-13 PPR repeat family
PPR PF01535 530 - 559 1.2e-07 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 744, 1166, 1233
Acc65I GGTACC 1 cut(s) 1752
AccB1I GGYRCC 2 cut(s) 407, 1752
AccI GTMKAC 3 cut(s) 629, 1141, 1303
AciI CCGC 1 cut(s) 86
AcsI RAATTY 4 cut(s) 361, 454, 622, 770
AfaI GTAC 8 cut(s) 51, 201, 215, 305, 794, 853, 1172, 1754
AflIII ACRYGT 1 cut(s) 959
AjnI CCWGG 1 cut(s) 8
AjuI GAANNNNNNNTTGG 4 cut(s) 452, 484, 552, 584
AluBI AGCT 9 cut(s) 320, 494, 902, 1000, 1115, 1333, 1367, 1535, 1773
AluI AGCT 9 cut(s) 320, 494, 902, 1000, 1115, 1333, 1367, 1535, 1773
AoxI GGCC 1 cut(s) 26
ApeKI GCWGC 3 cut(s) 902, 987, 1532
ApoI RAATTY 4 cut(s) 361, 454, 622, 770
AseI ATTAAT 3 cut(s) 975, 1080, 1181
Asp718I GGTACC 1 cut(s) 1752
AspS9I GGNCC 1 cut(s) 1678
AsuC2I CCSGG 1 cut(s) 54
AsuHPI GGTGA 4 cut(s) 509, 565, 575, 655
AvaII GGWCC 1 cut(s) 1678
AxyI CCTNAGG 1 cut(s) 23
BaeGI GKGCMC 1 cut(s) 1552
BanI GGYRCC 2 cut(s) 407, 1752
BanII GRGCYC 1 cut(s) 276
BbsI GAAGAC 2 cut(s) 64, 467
BbvI GCAGC 3 cut(s) 889, 974, 1544
BccI CCATC 8 cut(s) 144, 752, 1196, 1315, 1391, 1492, 1496, 1549
BciT130I CCWGG 1 cut(s) 10
BciVI GTATCC 1 cut(s) 1394
BclI TGATCA 3 cut(s) 645, 1452, 1602
BcnI CCSGG 1 cut(s) 54
BfaI CTAG 3 cut(s) 257, 1116, 1770
BfuI GTATCC 1 cut(s) 1394
BisI GCNGC 3 cut(s) 903, 988, 1533
BlsI GCNGC 3 cut(s) 904, 989, 1534
BmcAI AGTACT 1 cut(s) 201
Bme1390I CCNGG 2 cut(s) 10, 54
Bme18I GGWCC 1 cut(s) 1678
BmgT120I GGNCC 1 cut(s) 1678
BmiI GGNNCC 5 cut(s) 398, 409, 1155, 1680, 1754
BmrFI CCNGG 2 cut(s) 10, 54
BmrI ACTGGG 2 cut(s) 191, 460
BmsI GCATC 5 cut(s) 13, 88, 793, 1177, 1627
BmuI ACTGGG 2 cut(s) 191, 460
BpiI GAAGAC 2 cut(s) 64, 467
BpmI CTGGAG 2 cut(s) 207, 1043
BpuMI CCSGG 1 cut(s) 54
BsaJI CCNNGG 1 cut(s) 8
BsaXI ACNNNNNCTCC 2 cut(s) 1016, 1046
Bse1I ACTGG 2 cut(s) 197, 466
Bse21I CCTNAGG 1 cut(s) 23
Bse3DI GCAATG 1 cut(s) 352
BseBI CCWGG 1 cut(s) 10
BseDI CCNNGG 1 cut(s) 8
BseGI GGATG 8 cut(s) 4, 519, 694, 795, 1207, 1212, 1507, 1560
BseMI GCAATG 1 cut(s) 352
BseMII CTCAG 5 cut(s) 14, 174, 223, 1535, 1540
BseNI ACTGG 2 cut(s) 197, 466
BseRI GAGGAG 3 cut(s) 622, 692, 1266
BseSI GKGCMC 1 cut(s) 1552
BseXI GCAGC 3 cut(s) 889, 974, 1544
BsgI GTGCAG 1 cut(s) 1551
BshFI GGCC 1 cut(s) 28
BshNI GGYRCC 2 cut(s) 407, 1752
BsiSI CCGG 2 cut(s) 54, 1285
BslFI GGGAC 1 cut(s) 1664
BsmFI GGGAC 1 cut(s) 1664
BsnI GGCC 1 cut(s) 28
Bsp1286I GDGCHC 2 cut(s) 276, 1552
Bsp1407I TGTACA 1 cut(s) 792
Bsp143I GATC 6 cut(s) 253, 377, 645, 1369, 1452, 1602
BspACI CCGC 1 cut(s) 86
BspANI GGCC 1 cut(s) 28
BspCNI CTCAG 5 cut(s) 15, 173, 222, 1536, 1539
BspLI GGNNCC 5 cut(s) 398, 409, 1155, 1680, 1754
BspT107I GGYRCC 2 cut(s) 407, 1752
BsrDI GCAATG 1 cut(s) 352
BsrGI TGTACA 1 cut(s) 792
BsrI ACTGG 2 cut(s) 197, 466
BssECI CCNNGG 1 cut(s) 8
BssMI GATC 6 cut(s) 253, 377, 645, 1369, 1452, 1602
BssNAI GTATAC 1 cut(s) 630
Bst1107I GTATAC 1 cut(s) 630
Bst2UI CCWGG 1 cut(s) 10
Bst4CI ACNGT 2 cut(s) 520, 1406
Bst6I CTCTTC 2 cut(s) 1103, 1445
BstAUI TGTACA 1 cut(s) 792
BstC8I GCNNGC 1 cut(s) 46
BstDEI CTNAG 8 cut(s) 23, 160, 209, 277, 1197, 1526, 1544, 1776
BstF5I GGATG 8 cut(s) 4, 519, 694, 795, 1207, 1212, 1507, 1560
BstKTI GATC 6 cut(s) 256, 380, 648, 1372, 1455, 1605
BstMBI GATC 6 cut(s) 253, 377, 645, 1369, 1452, 1602
BstMWI GCNNNNNNNGC 1 cut(s) 10
BstNI CCWGG 1 cut(s) 10
BstNSI RCATGY 1 cut(s) 963
BstSCI CCNGG 2 cut(s) 8, 52
BstSLI GKGCMC 1 cut(s) 1552
BstV1I GCAGC 3 cut(s) 889, 974, 1544
BstV2I GAAGAC 2 cut(s) 64, 467
BstZ17I GTATAC 1 cut(s) 630
Bsu36I CCTNAGG 1 cut(s) 23
BsuI GTATCC 1 cut(s) 1394
BsuRI GGCC 1 cut(s) 28
BtsCI GGATG 8 cut(s) 4, 519, 694, 795, 1207, 1212, 1507, 1560
BtsIMutI CAGTG 1 cut(s) 1534
Cac8I GCNNGC 1 cut(s) 46
Cfr13I GGNCC 1 cut(s) 1678
Csp6I GTAC 8 cut(s) 50, 200, 214, 304, 793, 852, 1171, 1753
CspCI CAANNNNNGTGG 2 cut(s) 757, 792
CviAII CATG 4 cut(s) 329, 960, 1340, 1742
CviQI GTAC 8 cut(s) 50, 200, 214, 304, 793, 852, 1171, 1753
DdeI CTNAG 8 cut(s) 23, 160, 209, 277, 1197, 1526, 1544, 1776
DpnI GATC 6 cut(s) 255, 379, 647, 1371, 1454, 1604
DpnII GATC 6 cut(s) 253, 377, 645, 1369, 1452, 1602
Eam1104I CTCTTC 2 cut(s) 1103, 1445
EarI CTCTTC 2 cut(s) 1103, 1445
Eco147I AGGCCT 1 cut(s) 28
Eco24I GRGCYC 1 cut(s) 276
Eco32I GATATC 1 cut(s) 1765
Eco47I GGWCC 1 cut(s) 1678
Eco81I CCTNAGG 1 cut(s) 23
EcoRII CCWGG 1 cut(s) 8
EcoRV GATATC 1 cut(s) 1765
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 276
FaeI CATG 4 cut(s) 332, 963, 1343, 1745
FaqI GGGAC 1 cut(s) 1664
FatI CATG 4 cut(s) 328, 959, 1339, 1741
FauNDI CATATG 1 cut(s) 445
FbaI TGATCA 3 cut(s) 645, 1452, 1602
FblI GTMKAC 3 cut(s) 629, 1141, 1303
Fnu4HI GCNGC 3 cut(s) 903, 988, 1533
FokI GGATG 7 cut(s) 526, 701, 802, 1214, 1219, 1514, 1567
FriOI GRGCYC 1 cut(s) 276
Fsp4HI GCNGC 3 cut(s) 903, 988, 1533
FspBI CTAG 3 cut(s) 257, 1116, 1770
GluI GCNGC 3 cut(s) 903, 988, 1533
GsuI CTGGAG 2 cut(s) 207, 1043
HaeIII GGCC 1 cut(s) 28
HapII CCGG 2 cut(s) 54, 1285
Hin1II CATG 4 cut(s) 332, 963, 1343, 1745
HincII GTYRAC 2 cut(s) 427, 1304
HindII GTYRAC 2 cut(s) 427, 1304
HindIII AAGCTT 1 cut(s) 1331
HinfI GANTC 6 cut(s) 182, 261, 313, 1418, 1468, 1540
HpaI GTTAAC 1 cut(s) 427
HpaII CCGG 2 cut(s) 54, 1285
HphI GGTGA 4 cut(s) 509, 565, 575, 655
Hpy166II GTNNAC 7 cut(s) 427, 589, 630, 852, 957, 1142, 1304
Hpy188I TCNGA 5 cut(s) 70, 97, 124, 131, 1545
Hpy188III TCNNGA 4 cut(s) 22, 186, 1422, 1456
Hpy8I GTNNAC 7 cut(s) 427, 589, 630, 852, 957, 1142, 1304
HpyCH4III ACNGT 2 cut(s) 520, 1406
HpyCH4IV ACGT 1 cut(s) 1169
HpyF10VI GCNNNNNNNGC 1 cut(s) 10
HpyF3I CTNAG 8 cut(s) 23, 160, 209, 277, 1197, 1526, 1544, 1776
HpySE526I ACGT 1 cut(s) 1169
Hsp92II CATG 4 cut(s) 332, 963, 1343, 1745
KpnI GGTACC 1 cut(s) 1756
Ksp22I TGATCA 3 cut(s) 645, 1452, 1602
KspAI GTTAAC 1 cut(s) 427
Kzo9I GATC 6 cut(s) 253, 377, 645, 1369, 1452, 1602
LmnI GCTCC 4 cut(s) 188, 396, 997, 1153
Lsp1109I GCAGC 3 cut(s) 889, 974, 1544
LweI GCATC 5 cut(s) 13, 88, 793, 1177, 1627
MaeI CTAG 3 cut(s) 257, 1116, 1770
MaeII ACGT 1 cut(s) 1169
MaeIII GTNAC 1 cut(s) 530
MalI GATC 6 cut(s) 255, 379, 647, 1371, 1454, 1604
MboI GATC 6 cut(s) 253, 377, 645, 1369, 1452, 1602
MhlI GDGCHC 2 cut(s) 276, 1552
MlyI GAGTC 2 cut(s) 255, 1477
MmeI TCCRAC 2 cut(s) 654, 1286
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 9 cut(s) 218, 426, 615, 636, 699, 975, 1080, 1181, 1653
MslI CAYNNNNRTG 1 cut(s) 405
MspA1I CMGCKG 1 cut(s) 902
MspI CCGG 2 cut(s) 54, 1285
MspR9I CCNGG 2 cut(s) 10, 54
MvaI CCWGG 1 cut(s) 10
MwoI GCNNNNNNNGC 1 cut(s) 10
NciI CCSGG 1 cut(s) 54
NdeI CATATG 1 cut(s) 445
NdeII GATC 6 cut(s) 253, 377, 645, 1369, 1452, 1602
NlaIII CATG 4 cut(s) 332, 963, 1343, 1745
NlaIV GGNNCC 5 cut(s) 398, 409, 1155, 1680, 1754
NmeAIII GCCGAG 1 cut(s) 1182
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 963
PceI AGGCCT 1 cut(s) 28
PciI ACATGT 1 cut(s) 959
PfeI GAWTC 4 cut(s) 182, 313, 1418, 1540
PkrI GCNGC 3 cut(s) 904, 989, 1534
PleI GAGTC 2 cut(s) 255, 1476
PpsI GAGTC 2 cut(s) 255, 1476
PscI ACATGT 1 cut(s) 959
PshBI ATTAAT 3 cut(s) 975, 1080, 1181
PsiI TTATAA 3 cut(s) 744, 1166, 1233
Psp6I CCWGG 1 cut(s) 8
PspGI CCWGG 1 cut(s) 8
PspN4I GGNNCC 5 cut(s) 398, 409, 1155, 1680, 1754
PspPI GGNCC 1 cut(s) 1678
PvuII CAGCTG 1 cut(s) 902
RsaI GTAC 8 cut(s) 51, 201, 215, 305, 794, 853, 1172, 1754
RsaNI GTAC 8 cut(s) 50, 200, 214, 304, 793, 852, 1171, 1753
RseI CAYNNNNRTG 1 cut(s) 405
SalI GTCGAC 1 cut(s) 1302
SaqAI TTAA 9 cut(s) 218, 426, 615, 636, 699, 975, 1080, 1181, 1653
SatI GCNGC 3 cut(s) 903, 988, 1533
Sau3AI GATC 6 cut(s) 253, 377, 645, 1369, 1452, 1602
Sau96I GGNCC 1 cut(s) 1678
ScaI AGTACT 1 cut(s) 201
SchI GAGTC 2 cut(s) 255, 1477
ScrFI CCNGG 2 cut(s) 10, 54
SduI GDGCHC 2 cut(s) 276, 1552
SfaNI GCATC 5 cut(s) 13, 88, 793, 1177, 1627
SinI GGWCC 1 cut(s) 1678
SmiMI CAYNNNNRTG 1 cut(s) 405
SseBI AGGCCT 1 cut(s) 28
SsiI CCGC 1 cut(s) 86
SspI AATATT 1 cut(s) 862
SspMI CTAG 3 cut(s) 257, 1116, 1770
StuI AGGCCT 1 cut(s) 28
StyD4I CCNGG 2 cut(s) 8, 52
TaaI ACNGT 2 cut(s) 520, 1406
TaiI ACGT 1 cut(s) 1172
TaqI TCGA 3 cut(s) 316, 1271, 1303
TatI WGTACW 3 cut(s) 199, 792, 851
TfiI GAWTC 4 cut(s) 182, 313, 1418, 1540
Tru1I TTAA 9 cut(s) 218, 426, 615, 636, 699, 975, 1080, 1181, 1653
Tru9I TTAA 9 cut(s) 218, 426, 615, 636, 699, 975, 1080, 1181, 1653
TscAI CASTG 1 cut(s) 1534
TseI GCWGC 3 cut(s) 902, 987, 1532
TspDTI ATGAA 8 cut(s) 158, 273, 375, 722, 836, 1121, 1356, 1736
TspGWI ACGGA 1 cut(s) 1567
TspRI CASTG 1 cut(s) 1534
VpaK11BI GGWCC 1 cut(s) 1678
VspI ATTAAT 3 cut(s) 975, 1080, 1181
XapI RAATTY 4 cut(s) 361, 454, 622, 770
XceI RCATGY 1 cut(s) 963
XmiI GTMKAC 3 cut(s) 629, 1141, 1303
XspI CTAG 3 cut(s) 257, 1116, 1770
ZrmI AGTACT 1 cut(s) 201
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.