Rroxscaffold_2G00142790

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0008008
Physical Location & Seq
Forward (+)
80876070 .. 80882120
6051 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00142790.1

Sequence Viewer

Length: 3252 bp
ATGTTGAGGTGGGCTGCAGCACACCTCAGCCCACCCTTCCTTCCATGCCTAGTTCCCTCTCCTCTCCCCTTAACTAAGAGTTTTCTTTTCGAGGAGCACAAATACCTTGCCAAGCTATGTAAAGATGTGATTCATGAAGTGGGGAAGGAGAATGTGGTTCAAATATGTATCGATAATGGCTTAGCATTTGTGAAGGCTGGTAAAACATTATCGGAACGTTATAAATTCTATTGGACTTCATGTGCTGCTCACCGCATCTACTTAATGTTTAAAGATATTGGTAAAAAGGCAAAGGTAAGTCATGTGATTACTAATGCTAGACTCGTGACTGTATATATCTACAATCACGGATGGGTGCTTGCTCTTATGAGAAAAATATGTAAGGGTGATTTGATTCGACCGGGACCAACATGGTTTGCCACCGATTACATTTCTCTTCAAAGCCTTTTCCAAAAGAAAGCTGGACTTAAACAATTGTTTACTTGTGATGACTGGGCTACTAGCAAATATAGTCGAAGCAAGGAGGGAAAACGTATAGAGGAACTCGTTCTTGAAACCATGTTTTGGGACAATGTGCAAGCGCGTGATTTCTATTTACGAGCCATTATACGGATTCTTCGAATAGTTGACACAGAGGTTAACCCGACAATGCCAATGTTGTACGATTTATTTAAGAAGGAATTAGTGATGATCCGAGCTACTAGATGCCCTTCACTCGGTTTTGCAAAGTTAGACCTAAACTCCGTACGCATGGCACAATTCGGAAATGAGATTAGAAGCTTTAGAGACTCATTATACGGATTTGGAAGACTAGCTGTCATTGCTGCGAGGAAATCAATGACTGCATACGAATGGCGGAACATGTATGGGGGCGAAGCACCTACACTTCAAACAAGTAAGAGATCATTCGATGAAAGTGATGGAAGTGGTTATAAATTATCAAGAGGCACTAGTGAAGAATCAATTGATAATAATGATGATGATGATGATGATGGTGGTGGTGGTGGTGGTGGTGATGGTGATAGTGGTAATACGGTGGTGGTAGCAGATATCGTGGCTATGGTGGTAGTGGACGTGATAACTCGTGTGACAATAGTAATTATGACGACACTAATCGTGAGGCAAATTATGGAAATGACGGGGAGTGGCAAATCATGGCACTACAACCAATATAGTAGTGATGATCATACTACTTATCCTGATGCAAATCGTGAATACAATTCTAGAGGTAGGGATACACAAGGAAGACGCGATAGCCATTTGTCTCTAAATGAGTTTGAATCACTTTCTTCAAGTCTTGGCTCAATGGACATAGGAACTCAATATAGTGATAACCCAAACCCATTCCATTTTCATTATCCTCATCATGAAATGAGTTCTACCTCGAAAGTACATCCGGAATTGGATCAAGCTCACAAGGTGGAAGCACATATGGCCTTTTTGATCATTCTTCGTCCCAAATGCCTTTCAATTTCCTCCATTAACAAAATGCTAAAGCTCCTCTCCTCCTCCTCATCATGCCTCCACGCTCGAGCCCATATTCAGCAGCACCCCCAAATCAACATAATCAAACTCTGTTCCACAATCTCCATCTCCGAAAATCCAAGCTCACAGTCCTTCAATGTTCCATTAGTTTCAGACCTCATAGCAAAGCAACACTGGTCAGAGCTGAAAACCCATCTCAAAGACTCAACCTTTATCACAGTTCTTCACCAACTGATCGATTCTGGAGCTGACCCAGAGCTCATACTCAGGTACTTCAATTGGGCCCAGAAGGGTCAAAATGTTCCACACAATATTGAAATCACTTGTAGACTATTGCAGTCACTTTCAAATGCAAAGAAGTACTCCAAGATGAGAGCTTTCTTAGATGAGTTTGTTAGAAGGAATGAGAAGCATTCAGTTTCTTCAATTTTTCACATGCTTTCAATGTCTGGCAATCACTTTTGTGCTAATTCCATTATAGTTGATATGCTGGTGTTGGCTTATGTTAAGAATTCGAAGACCAATTCGGGTTTTGAGGCGTTTCAGAGAGCTGGTGATTATGGGTGTAGGCTGTCAAAACTGTCTTGTAATGCTTTGTTGAGTGCTTTGGTGAAGGAAAATGAAATTGGGTATGTTGAGTTTGTGTACAAGGAGATGTTTAGGAGGAGGATAGAGGCGGATTTGTATAGTTTCAATATTGTGATTAATGGGTTGTGTAAAGTTGGGAAATTGAACAAGGCAAAGGATGTTATGGATGATATGAAGGCTTGGGGAATGCATAAGGCTGATGCCATATTGGAGGAGATGGTGGCGAACAATATTTGTCCAAGTGTGATCACGTTCAATATTTTGATTGATGGGTTCTGTAAAGAAGATGAGAATGTAGCAGCTGCGTTTAAGGTGTTTGAGGAAATGAGGAGGCAGGGCTTGAAACCTAATGTGGTTACTTATAATTCACTGATTAATGGGTTATGCTGTGATGGGAAGCTTGAGGAGGCTTGTGGATTGCGGGATGAAATGCTTAGATTGGGTTTGAAGCCAGAGATAGCAACCTACAATGCACTTATAAATGGGTTTTGCAAGAAGAAGATGCTGAAGGAAGCTAGAGAGCTGTTTGATAACATTTGTGAGCGAGGCTTGGTAACCAATGTTATAACATTTAATTCACTAATTGATGCTTATTGTAAGAATGAGATGAAGGAGGAAGCATATGCATTGCATAACATGATGATAGAGAGAGGGGTTTTACCAAACACGTCGACATTTAACTGCTTGATTCATGGTTCTCTTAGACAGGGAAACATAGAAACAGCCAGGAAATTCTTACAACAAATGGAGGCTGAGGGCTTGAAAGCTGATTGCATAACATATGATATACTGATAAATGCATTATGTAAAGATGGGGAGCCAAGAAAGGCAGAAAGACTCCTGAATGAGATGTTCACGAAGGGTTTGAGCCCAAGGCACGTGACATACAATACTTTGATGGATGGCTACTGCAGGGAAGGAAGCCTGAAGGCAGCTTTGAACTTGAGGACTCGGATGGAGAAAGAAGGGAAACGAGCAAATATAGCAACATACAATGTGCTGATCAAGGGATATTGTATGCAGGACAAGCTTGTAGATGCGAATGTACTTCTAAATGAAATGTTGGAGAAAGGTTTGATCCCAAATCAAACTACTTATGAAATAGTTAAAGTGGAAATGATGGACAAAGGTTTTGTTCCTGATATAGATGGACACCTGCATAATATCTCTAGCTCTTAA

Protein Analysis

1083

Amino Acids

122.56

Weight (kDa)

8.17

Isoelectric Point (pI)

40.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF659 PF04937 30 - 109 9.7e-20 Domain of unknown function (DUF659)
PPR_2 PF13041 690 - 735 1.3e-08 PPR repeat family
PPR_1 PF12854 719 - 750 1.1e-08 PPR repeat
PPR PF01535 724 - 753 4.2e-06 PPR repeat
PPR_2 PF13041 725 - 754 1.4e-06 PPR repeat family
PPR_2 PF13041 754 - 785 6.3e-07 PPR repeat family
PPR_long PF17177 758 - 865 9.4e-07 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 760 - 817 1e-13 Pentatricopeptide repeat domain
PPR_1 PF12854 768 - 801 3.7e-10 PPR repeat
PPR_2 PF13041 771 - 820 1.7e-19 PPR repeat family
TPR_24 PF23276 773 - 869 1.6e-06 Fungal tetratrico peptide repeats
PPR_1 PF12854 804 - 835 1.3e-14 PPR repeat
PPR_2 PF13041 807 - 856 2.4e-17 PPR repeat family
PPR PF01535 810 - 840 1.4e-08 PPR repeat
PPR_3 PF13812 832 - 892 7.8e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 839 - 868 1.1e-09 PPR repeat
PPR_2 PF13041 842 - 885 4.7e-11 PPR repeat family
PPR PF01535 846 - 875 1.8e-07 PPR repeat
TPR_24 PF23276 865 - 964 8.9e-07 Fungal tetratrico peptide repeats
PPR_1 PF12854 873 - 905 2.2e-07 PPR repeat
PPR_2 PF13041 878 - 923 8.3e-14 PPR repeat family
PPR PF01535 880 - 910 6.8e-08 PPR repeat
PPR_3 PF13812 905 - 959 2.9e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 908 - 941 2.8e-06 PPR repeat
PPR_2 PF13041 912 - 961 1.2e-13 PPR repeat family
TPR_24 PF23276 926 - 1035 3.2e-08 Fungal tetratrico peptide repeats
PPR_long PF17177 929 - 1026 2.5e-06 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 937 - 994 3.1e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 944 - 975 2.9e-10 PPR repeat
PPR PF01535 950 - 980 1.3e-06 PPR repeat
PPR_2 PF13041 950 - 987 1.7e-08 PPR repeat family
PPR_3 PF13812 970 - 1029 5e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 978 - 1011 1.7e-09 PPR repeat
PPR_2 PF13041 984 - 1030 9.4e-15 PPR repeat family
PPR PF01535 985 - 1014 1.3e-06 PPR repeat
PPR_1 PF12854 1017 - 1045 9.8e-06 PPR repeat
PPR_2 PF13041 1018 - 1059 2e-11 PPR repeat family
PPR PF01535 1021 - 1050 7.6e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 5 cut(s) 222, 933, 2436, 2552, 2639
AarI CACCTGC 1 cut(s) 3237
Acc36I ACCTGC 1 cut(s) 3237
AccB7I CCANNNNNTGG 1 cut(s) 564
AccI GTMKAC 2 cut(s) 1813, 2744
AccII CGCG 2 cut(s) 583, 1251
AccIII TCCGGA 1 cut(s) 1396
AciI CCGC 4 cut(s) 253, 856, 2162, 2494
AclI AACGTT 1 cut(s) 217
AclWI GGATC 3 cut(s) 685, 1413, 3145
AcsI RAATTY 3 cut(s) 224, 1996, 2804
AcuI CTGAAG 2 cut(s) 2600, 3020
AcvI CACGTG 1 cut(s) 2953
AdeI CACNNNGTG 1 cut(s) 1420
AfaI GTAC 7 cut(s) 662, 747, 1392, 1757, 1847, 2132, 3120
AfiI CCNNNNNNNGG 3 cut(s) 564, 609, 716
AflIII ACRYGT 2 cut(s) 861, 2739
AhdI GACNNNNNGTC 1 cut(s) 815
AhlI ACTAGT 1 cut(s) 950
AjiI CACGTC 2 cut(s) 1075, 2742
AjnI CCWGG 1 cut(s) 2798
AjuI GAANNNNNNNTTGG 2 cut(s) 2094, 2126
AleI CACNNNNGTG 1 cut(s) 1947
Alw21I GWGCWC 2 cut(s) 99, 1746
Alw26I GTCTC 2 cut(s) 780, 1269
AlwI GGATC 3 cut(s) 685, 1413, 3145
Ama87I CYCGRG 1 cut(s) 1530
Aor13HI TCCGGA 1 cut(s) 1396
AoxI GGCC 2 cut(s) 1434, 1767
ApaI GGGCCC 1 cut(s) 1771
ApeKI GCWGC 8 cut(s) 14, 17, 245, 824, 1546, 2372, 2375, 3005
ApoI RAATTY 3 cut(s) 224, 1996, 2804
ArsI GACNNNNNNTTYG 2 cut(s) 3188, 3220
AseI ATTAAT 2 cut(s) 2190, 2448
Asp700I GAANNNNTTC 1 cut(s) 546
AspLEI GCGC 1 cut(s) 583
AspS9I GGNCC 3 cut(s) 404, 1767, 1768
AsuC2I CCSGG 1 cut(s) 402
AsuHPI GGTGA 7 cut(s) 242, 398, 1025, 1031, 1703, 2051, 2107
AsuII TTCGAA 2 cut(s) 619, 2000
AvaI CYCGRG 1 cut(s) 1530
AvaII GGWCC 1 cut(s) 404
BaeGI GKGCMC 1 cut(s) 1771
BanII GRGCYC 4 cut(s) 1537, 1746, 1771, 2945
BauI CACGAG 2 cut(s) 323, 1083
BbrPI CACGTG 1 cut(s) 2953
BbsI GAAGAC 3 cut(s) 814, 1252, 2009
Bbv12I GWGCWC 2 cut(s) 99, 1746
BbvCI CCTCAGC 2 cut(s) 26, 2826
BbvI GCAGC 7 cut(s) 29, 232, 811, 1558, 2362, 2384, 3017
BciT130I CCWGG 1 cut(s) 2800
BciVI GTATCC 1 cut(s) 1228
BclI TGATCA 4 cut(s) 1183, 1443, 2319, 3075
BcnI CCSGG 1 cut(s) 402
BcoDI GTCTC 2 cut(s) 780, 1269
BcuI ACTAGT 1 cut(s) 950
BfaI CTAG 9 cut(s) 50, 318, 501, 702, 812, 951, 1224, 2589, 3243
BfmI CTRYAG 2 cut(s) 15, 2983
BfuAI ACCTGC 1 cut(s) 3237
BfuI GTATCC 1 cut(s) 1228
BisI GCNGC 8 cut(s) 15, 18, 246, 825, 1547, 2373, 2376, 3006
BlpI GCTNAGC 1 cut(s) 181
BlsI GCNGC 8 cut(s) 16, 19, 247, 826, 1548, 2374, 2377, 3007
BmcAI AGTACT 1 cut(s) 1847
Bme1390I CCNGG 2 cut(s) 402, 2800
Bme18I GGWCC 1 cut(s) 404
BmeRI GACNNNNNGTC 1 cut(s) 815
BmeT110I CYCGRG 1 cut(s) 1530
BmgBI CACGTC 2 cut(s) 1075, 2742
BmgT120I GGNCC 3 cut(s) 404, 1767, 1768
BmiI GGNNCC 3 cut(s) 405, 1769, 2892
BmrFI CCNGG 2 cut(s) 402, 2800
BmrI ACTGGG 1 cut(s) 502
BmsI GCATC 7 cut(s) 264, 695, 1192, 2263, 2565, 2650, 3100
BmuI ACTGGG 1 cut(s) 502
BpiI GAAGAC 3 cut(s) 814, 1252, 2009
BpmI CTGGAG 1 cut(s) 1749
Bpu10I CCTNAGC 2 cut(s) 26, 2826
Bpu1102I GCTNAGC 1 cut(s) 181
Bpu14I TTCGAA 2 cut(s) 619, 2000
BpuEI CTTGAG 2 cut(s) 2495, 3037
BpuMI CCSGG 1 cut(s) 402
Bsa29I ATCGAT 2 cut(s) 171, 1722
BsaAI YACGTR 1 cut(s) 2953
BsaBI GATNNNNATC 1 cut(s) 1206
BsaJI CCNNGG 1 cut(s) 2945
BsaWI WCCGGW 1 cut(s) 1396
BsaXI ACNNNNNCTCC 2 cut(s) 725, 755
Bsc4I CCNNNNNNNGG 3 cut(s) 564, 609, 716
Bse1I ACTGG 2 cut(s) 497, 1664
Bse3DI GCAATG 2 cut(s) 819, 2699
Bse8I GATNNNNATC 1 cut(s) 1206
BseAI TCCGGA 1 cut(s) 1396
BseBI CCWGG 1 cut(s) 2800
BseCI ATCGAT 2 cut(s) 171, 1722
BseDI CCNNGG 1 cut(s) 2945
BseGI GGATG 7 cut(s) 356, 1393, 2236, 2245, 2503, 2980, 3033
BseJI GATNNNNATC 1 cut(s) 1206
BseLI CCNNNNNNNGG 3 cut(s) 564, 609, 716
BseMI GCAATG 2 cut(s) 819, 2699
BseMII CTCAG 3 cut(s) 40, 1765, 2817
BseNI ACTGG 2 cut(s) 497, 1664
BseSI GKGCMC 1 cut(s) 1771
BseXI GCAGC 7 cut(s) 29, 232, 811, 1558, 2362, 2384, 3017
Bsh1236I CGCG 2 cut(s) 583, 1251
Bsh1285I CGRYCG 1 cut(s) 401
BshFI GGCC 2 cut(s) 1436, 1769
BshVI ATCGAT 2 cut(s) 171, 1722
BsiEI CGRYCG 1 cut(s) 401
BsiHKAI GWGCWC 2 cut(s) 99, 1746
BsiHKCI CYCGRG 1 cut(s) 1530
BsiSI CCGG 2 cut(s) 401, 1397
BsiWI CGTACG 1 cut(s) 745
BslFI GGGAC 3 cut(s) 417, 581, 1440
BslI CCNNNNNNNGG 3 cut(s) 564, 609, 716
BsmAI GTCTC 2 cut(s) 780, 1269
BsmFI GGGAC 3 cut(s) 417, 581, 1440
BsmI GAATGC 2 cut(s) 1897, 2265
BsnI GGCC 2 cut(s) 1436, 1769
BsoBI CYCGRG 1 cut(s) 1530
Bsp119I TTCGAA 2 cut(s) 619, 2000
Bsp120I GGGCCC 1 cut(s) 1767
Bsp1286I GDGCHC 5 cut(s) 99, 1537, 1746, 1771, 2945
Bsp13I TCCGGA 1 cut(s) 1396
Bsp1407I TGTACA 1 cut(s) 2130
Bsp143I GATC 9 cut(s) 690, 902, 1183, 1405, 1443, 1719, 2319, 3075, 3150
Bsp1720I GCTNAGC 1 cut(s) 181
BspACI CCGC 4 cut(s) 253, 856, 2162, 2494
BspANI GGCC 2 cut(s) 1436, 1769
BspCNI CTCAG 3 cut(s) 39, 1764, 2818
BspDI ATCGAT 2 cut(s) 171, 1722
BspEI TCCGGA 1 cut(s) 1396
BspFNI CGCG 2 cut(s) 583, 1251
BspHI TCATGA 2 cut(s) 133, 1366
BspLI GGNNCC 3 cut(s) 405, 1769, 2892
BspMAI CTGCAG 2 cut(s) 19, 2987
BspMI ACCTGC 1 cut(s) 3237
BspPI GGATC 3 cut(s) 685, 1413, 3145
BspT104I TTCGAA 2 cut(s) 619, 2000
BsrDI GCAATG 2 cut(s) 819, 2699
BsrGI TGTACA 1 cut(s) 2130
BsrI ACTGG 2 cut(s) 497, 1664
BssECI CCNNGG 1 cut(s) 2945
BssMI GATC 9 cut(s) 690, 902, 1183, 1405, 1443, 1719, 2319, 3075, 3150
BssSI CACGAG 2 cut(s) 323, 1083
BssT1I CCWWGG 1 cut(s) 2945
Bst2BI CACGAG 2 cut(s) 323, 1083
Bst2UI CCWGG 1 cut(s) 2800
Bst4CI ACNGT 5 cut(s) 331, 1036, 1614, 1705, 2067
Bst6I CTCTTC 1 cut(s) 441
BstAUI TGTACA 1 cut(s) 2130
BstBAI YACGTR 1 cut(s) 2953
BstBI TTCGAA 2 cut(s) 619, 2000
BstC8I GCNNGC 2 cut(s) 360, 579
BstDEI CTNAG 8 cut(s) 26, 75, 181, 1751, 1867, 2507, 2774, 2826
BstEII GGTNACC 1 cut(s) 2626
BstF5I GGATG 7 cut(s) 356, 1393, 2236, 2245, 2503, 2980, 3033
BstFNI CGCG 2 cut(s) 583, 1251
BstHHI GCGC 1 cut(s) 583
BstKTI GATC 9 cut(s) 693, 905, 1186, 1408, 1446, 1722, 2322, 3078, 3153
BstMAI GTCTC 2 cut(s) 780, 1269
BstMBI GATC 9 cut(s) 690, 902, 1183, 1405, 1443, 1719, 2319, 3075, 3150
BstMCI CGRYCG 1 cut(s) 401
BstMWI GCNNNNNNNGC 4 cut(s) 821, 1433, 3056, 3100
BstNI CCWGG 1 cut(s) 2800
BstNSI RCATGY 2 cut(s) 865, 1924
BstPI GGTNACC 1 cut(s) 2626
BstSCI CCNGG 2 cut(s) 400, 2798
BstSFI CTRYAG 2 cut(s) 15, 2983
BstSLI GKGCMC 1 cut(s) 1771
BstUI CGCG 2 cut(s) 583, 1251
BstV1I GCAGC 7 cut(s) 29, 232, 811, 1558, 2362, 2384, 3017
BstV2I GAAGAC 3 cut(s) 814, 1252, 2009
Bsu15I ATCGAT 2 cut(s) 171, 1722
BsuI GTATCC 1 cut(s) 1228
BsuRI GGCC 2 cut(s) 1436, 1769
BsuTUI ATCGAT 2 cut(s) 171, 1722
BtrI CACGTC 2 cut(s) 1075, 2742
BtsCI GGATG 7 cut(s) 356, 1393, 2236, 2245, 2503, 2980, 3033
BtsIMutI CAGTG 2 cut(s) 1657, 2441
BveI ACCTGC 1 cut(s) 3237
Cac8I GCNNGC 2 cut(s) 360, 579
CciI TCATGA 2 cut(s) 133, 1366
CfoI GCGC 1 cut(s) 583
Cfr13I GGNCC 3 cut(s) 404, 1767, 1768
ClaI ATCGAT 2 cut(s) 171, 1722
CseI GACGC 1 cut(s) 1257
Csp6I GTAC 7 cut(s) 661, 746, 1391, 1756, 1846, 2131, 3119
CviQI GTAC 7 cut(s) 661, 746, 1391, 1756, 1846, 2131, 3119
DdeI CTNAG 8 cut(s) 26, 75, 181, 1751, 1867, 2507, 2774, 2826
DpnI GATC 9 cut(s) 692, 904, 1185, 1407, 1445, 1721, 2321, 3077, 3152
DpnII GATC 9 cut(s) 690, 902, 1183, 1405, 1443, 1719, 2319, 3075, 3150
DraI TTTAAA 1 cut(s) 271
DraIII CACNNNGTG 1 cut(s) 1420
DriI GACNNNNNGTC 1 cut(s) 815
Eam1104I CTCTTC 1 cut(s) 441
Eam1105I GACNNNNNGTC 1 cut(s) 815
EarI CTCTTC 1 cut(s) 441
EciI GGCGGA 2 cut(s) 871, 2177
Ecl136II GAGCTC 1 cut(s) 1744
Eco130I CCWWGG 1 cut(s) 2945
Eco24I GRGCYC 4 cut(s) 1537, 1746, 1771, 2945
Eco32I GATATC 1 cut(s) 1051
Eco47I GGWCC 1 cut(s) 404
Eco53kI GAGCTC 1 cut(s) 1744
Eco57I CTGAAG 2 cut(s) 2600, 3020
Eco72I CACGTG 1 cut(s) 2953
Eco88I CYCGRG 1 cut(s) 1530
Eco91I GGTNACC 1 cut(s) 2626
EcoICRI GAGCTC 1 cut(s) 1744
EcoO65I GGTNACC 1 cut(s) 2626
EcoRI GAATTC 1 cut(s) 1996
EcoRII CCWGG 1 cut(s) 2798
EcoRV GATATC 1 cut(s) 1051
EcoT14I CCWWGG 1 cut(s) 2945
EcoT22I ATGCAT 3 cut(s) 2265, 2701, 2875
EcoT38I GRGCYC 4 cut(s) 1537, 1746, 1771, 2945
ErhI CCWWGG 1 cut(s) 2945
FalI AAGNNNNNCTT 2 cut(s) 450, 482
FaqI GGGAC 3 cut(s) 417, 581, 1440
FauI CCCGC 1 cut(s) 2487
FauNDI CATATG 3 cut(s) 1431, 2695, 2854
FbaI TGATCA 4 cut(s) 1183, 1443, 2319, 3075
FblI GTMKAC 2 cut(s) 1813, 2744
Fnu4HI GCNGC 8 cut(s) 15, 18, 246, 825, 1547, 2373, 2376, 3006
FokI GGATG 7 cut(s) 363, 1380, 2243, 2252, 2510, 2987, 3040
FriOI GRGCYC 4 cut(s) 1537, 1746, 1771, 2945
Fsp4HI GCNGC 8 cut(s) 15, 18, 246, 825, 1547, 2373, 2376, 3006
FspBI CTAG 9 cut(s) 50, 318, 501, 702, 812, 951, 1224, 2589, 3243
GlaI GCGC 1 cut(s) 582
GluI GCNGC 8 cut(s) 15, 18, 246, 825, 1547, 2373, 2376, 3006
GsuI CTGGAG 1 cut(s) 1749
HaeIII GGCC 2 cut(s) 1436, 1769
HapII CCGG 2 cut(s) 401, 1397
HgaI GACGC 1 cut(s) 1257
HhaI GCGC 1 cut(s) 583
Hin6I GCGC 1 cut(s) 581
HinP1I GCGC 1 cut(s) 581
HincII GTYRAC 3 cut(s) 628, 640, 2745
HindII GTYRAC 3 cut(s) 628, 640, 2745
HindIII AAGCTT 3 cut(s) 778, 2471, 3101
HpaI GTTAAC 1 cut(s) 640
HpaII CCGG 2 cut(s) 401, 1397
HphI GGTGA 7 cut(s) 242, 398, 1025, 1031, 1703, 2051, 2107
Hpy166II GTNNAC 8 cut(s) 480, 628, 640, 1072, 1814, 2131, 2745, 2928
Hpy188I TCNGA 8 cut(s) 214, 695, 764, 1597, 1639, 1666, 2031, 3027
Hpy8I GTNNAC 8 cut(s) 480, 628, 640, 1072, 1814, 2131, 2745, 2928
Hpy99I CGWCG 1 cut(s) 2746
HpyCH4III ACNGT 5 cut(s) 331, 1036, 1614, 1705, 2067
HpyCH4IV ACGT 6 cut(s) 217, 532, 1074, 2324, 2741, 2952
HpyF10VI GCNNNNNNNGC 4 cut(s) 821, 1433, 3056, 3100
HpyF3I CTNAG 8 cut(s) 26, 75, 181, 1751, 1867, 2507, 2774, 2826
HpySE526I ACGT 6 cut(s) 217, 532, 1074, 2324, 2741, 2952
HspAI GCGC 1 cut(s) 581
Kpn2I TCCGGA 1 cut(s) 1396
Ksp22I TGATCA 4 cut(s) 1183, 1443, 2319, 3075
KspAI GTTAAC 1 cut(s) 640
Kzo9I GATC 9 cut(s) 690, 902, 1183, 1405, 1443, 1719, 2319, 3075, 3150
LmnI GCTCC 4 cut(s) 94, 1503, 1730, 2890
Lsp1109I GCAGC 7 cut(s) 29, 232, 811, 1558, 2362, 2384, 3017
LweI GCATC 7 cut(s) 264, 695, 1192, 2263, 2565, 2650, 3100
MaeI CTAG 9 cut(s) 50, 318, 501, 702, 812, 951, 1224, 2589, 3243
MaeII ACGT 6 cut(s) 217, 532, 1074, 2324, 2741, 2952
MaeIII GTNAC 6 cut(s) 325, 1087, 1824, 2428, 2626, 2953
MalI GATC 9 cut(s) 692, 904, 1185, 1407, 1445, 1721, 2321, 3077, 3152
MboI GATC 9 cut(s) 690, 902, 1183, 1405, 1443, 1719, 2319, 3075, 3150
MfeI CAATTG 3 cut(s) 473, 963, 1762
MhlI GDGCHC 5 cut(s) 99, 1537, 1746, 1771, 2945
MlyI GAGTC 5 cut(s) 315, 782, 1682, 2904, 3016
MmeI TCCRAC 1 cut(s) 3117
Mph1103I ATGCAT 3 cut(s) 2265, 2701, 2875
MroI TCCGGA 1 cut(s) 1396
MroXI GAANNNNTTC 1 cut(s) 546
MslI CAYNNNNRTG 2 cut(s) 850, 1947
MspA1I CMGCKG 1 cut(s) 2375
MspI CCGG 2 cut(s) 401, 1397
MspR9I CCNGG 2 cut(s) 402, 2800
MunI CAATTG 3 cut(s) 473, 963, 1762
Mva1269I GAATGC 2 cut(s) 1897, 2265
MvaI CCWGG 1 cut(s) 2800
MvnI CGCG 2 cut(s) 583, 1251
MwoI GCNNNNNNNGC 4 cut(s) 821, 1433, 3056, 3100
NciI CCSGG 1 cut(s) 402
NdeI CATATG 3 cut(s) 1431, 2695, 2854
NdeII GATC 9 cut(s) 690, 902, 1183, 1405, 1443, 1719, 2319, 3075, 3150
NlaIV GGNNCC 3 cut(s) 405, 1769, 2892
NmuCI GTSAC 4 cut(s) 325, 1087, 1824, 2953
NsiI ATGCAT 3 cut(s) 2265, 2701, 2875
NspI RCATGY 2 cut(s) 865, 1924
NspV TTCGAA 2 cut(s) 619, 2000
OliI CACNNNNGTG 1 cut(s) 1947
PaeR7I CTCGAG 1 cut(s) 1530
PagI TCATGA 2 cut(s) 133, 1366
PaqCI CACCTGC 1 cut(s) 3237
PciI ACATGT 1 cut(s) 861
PcsI WCGNNNNNNNCGW 1 cut(s) 616
PctI GAATGC 2 cut(s) 1897, 2265
PdmI GAANNNNTTC 1 cut(s) 546
PfeI GAWTC 7 cut(s) 130, 394, 613, 959, 1280, 1724, 2761
Pfl23II CGTACG 1 cut(s) 745
PflMI CCANNNNNTGG 1 cut(s) 564
PkrI GCNGC 8 cut(s) 16, 19, 247, 826, 1548, 2374, 2377, 3007
PleI GAGTC 5 cut(s) 315, 782, 1682, 2904, 3016
PmaCI CACGTG 1 cut(s) 2953
PmlI CACGTG 1 cut(s) 2953
PpsI GAGTC 5 cut(s) 315, 782, 1682, 2904, 3016
Ppu21I YACGTR 1 cut(s) 2953
PscI ACATGT 1 cut(s) 861
PshBI ATTAAT 2 cut(s) 2190, 2448
PsiI TTATAA 5 cut(s) 222, 933, 2436, 2552, 2639
Psp124BI GAGCTC 1 cut(s) 1746
Psp1406I AACGTT 1 cut(s) 217
Psp6I CCWGG 1 cut(s) 2798
PspCI CACGTG 1 cut(s) 2953
PspEI GGTNACC 1 cut(s) 2626
PspGI CCWGG 1 cut(s) 2798
PspLI CGTACG 1 cut(s) 745
PspN4I GGNNCC 3 cut(s) 405, 1769, 2892
PspOMI GGGCCC 1 cut(s) 1767
PspPI GGNCC 3 cut(s) 404, 1767, 1768
PspXI VCTCGAGB 1 cut(s) 1530
PstI CTGCAG 2 cut(s) 19, 2987
PvuII CAGCTG 1 cut(s) 2375
RsaI GTAC 7 cut(s) 662, 747, 1392, 1757, 1847, 2132, 3120
RsaNI GTAC 7 cut(s) 661, 746, 1391, 1756, 1846, 2131, 3119
RseI CAYNNNNRTG 2 cut(s) 850, 1947
SacI GAGCTC 1 cut(s) 1746
SalI GTCGAC 1 cut(s) 2743
SatI GCNGC 8 cut(s) 15, 18, 246, 825, 1547, 2373, 2376, 3006
Sau3AI GATC 9 cut(s) 690, 902, 1183, 1405, 1443, 1719, 2319, 3075, 3150
Sau96I GGNCC 3 cut(s) 404, 1767, 1768
ScaI AGTACT 1 cut(s) 1847
SchI GAGTC 5 cut(s) 315, 782, 1682, 2904, 3016
ScrFI CCNGG 2 cut(s) 402, 2800
SduI GDGCHC 5 cut(s) 99, 1537, 1746, 1771, 2945
SfaNI GCATC 7 cut(s) 264, 695, 1192, 2263, 2565, 2650, 3100
SfcI CTRYAG 2 cut(s) 15, 2983
Sfr274I CTCGAG 1 cut(s) 1530
SfuI TTCGAA 2 cut(s) 619, 2000
SinI GGWCC 1 cut(s) 404
SlaI CTCGAG 1 cut(s) 1530
SmiMI CAYNNNNRTG 2 cut(s) 850, 1947
SmlI CTYRAG 3 cut(s) 1530, 2474, 3016
SmoI CTYRAG 3 cut(s) 1530, 2474, 3016
SpeI ACTAGT 1 cut(s) 950
SsiI CCGC 4 cut(s) 253, 856, 2162, 2494
SspI AATATT 4 cut(s) 1798, 2182, 2305, 2332
SspMI CTAG 9 cut(s) 50, 318, 501, 702, 812, 951, 1224, 2589, 3243
SstI GAGCTC 1 cut(s) 1746
StyD4I CCNGG 2 cut(s) 400, 2798
StyI CCWWGG 1 cut(s) 2945
TaaI ACNGT 5 cut(s) 331, 1036, 1614, 1705, 2067
TaiI ACGT 6 cut(s) 220, 535, 1077, 2327, 2744, 2955
TatI WGTACW 4 cut(s) 1390, 1845, 2130, 3118
TfiI GAWTC 7 cut(s) 130, 394, 613, 959, 1280, 1724, 2761
TscAI CASTG 2 cut(s) 1664, 2448
TseFI GTSAC 4 cut(s) 325, 1087, 1824, 2953
TseI GCWGC 8 cut(s) 14, 17, 245, 824, 1546, 2372, 2375, 3005
Tsp45I GTSAC 4 cut(s) 325, 1087, 1824, 2953
TspGWI ACGGA 4 cut(s) 363, 625, 733, 813
TspRI CASTG 2 cut(s) 1664, 2448
Van91I CCANNNNNTGG 1 cut(s) 564
VpaK11BI GGWCC 1 cut(s) 404
VspI ATTAAT 2 cut(s) 2190, 2448
XapI RAATTY 3 cut(s) 224, 1996, 2804
XbaI TCTAGA 1 cut(s) 1223
XceI RCATGY 2 cut(s) 865, 1924
XcmI CCANNNNNNNNNTGG 1 cut(s) 458
XhoI CTCGAG 1 cut(s) 1530
XmiI GTMKAC 2 cut(s) 1813, 2744
XmnI GAANNNNTTC 1 cut(s) 546
XspI CTAG 9 cut(s) 50, 318, 501, 702, 812, 951, 1224, 2589, 3243
ZrmI AGTACT 1 cut(s) 1847
Zsp2I ATGCAT 3 cut(s) 2265, 2701, 2875
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.