RchiOBHm_Chr3g0457031

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0008008
Physical Location & Seq
Forward (+)
6293308 .. 6295688
2381 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42380

Sequence Viewer

Length: 1800 bp
ATGCATCCCAGACTTCAAATTCTCCACAGAAGCAAACAGATTGTGAGGACTCTCTGCTCTTTAACTTGCGTACCCAGAAATCCCACATCGGAAAAATTGAATCTTCCACTGGTTTCAGAGCTATTAGCAAAGCAGAGGTGGTCAGAGCTCAGAACCCATCTCAAAGACTCGGACTTTACCTCTGTTTTTCATCAGGTTTTGGATTCTGGAGCAGACCCAGTACTGATACTCAGGTACTTACATTGTTGCCAGAAGGATTTCAATGCTGCACATAATCTAGAACTCACTTTTAGACTCTTGGGTTCATTAGCAATAGCCAAAAGGTACTCAAAGATTAGAGCTTTTTTACATGGGTTCGTTAGAAGCAATGAAAAGCTATCAAATTCTGCAATTTTTAACTCGCTTTCGTTGGGTAGTAAACATTTGGCTGCAAATTCCATTATTGTTAACTTGTTGGTGTTGGCATATGCGAAAAATTTGAAGACCCATTTGGGTGTTGAAGCATTTCAGAAAGCTGGTGATTATGGGTTCAAGTTGGTGGTGTTTTCTTGTAACCCTTTGTTAAGGGCTTTGGTGAAGGAAGGTGAAATTGGGAATGTTGAATTTGTTTACAGGGAGATGATTAGGAGGAGAATTAGGCCCAATTTGTACACGTTTAATATGGTGATTAGGGGATTGTGTAAAGTTGGGAAATTGGAGGAGGCAAAGGATGTTATTATTAATGACATGAAGGCTTGGGGGATATCACCAAATGTGGTTAGTTATAATACTTTGATCGATGGGTATTGCAAGTTGGGTGGGTTGGGGAAGATGTACAAAGCTGATGCGATTGTGAAGGAAATGATGGCAAATAATATTCGGCCAAATAAGTGTACTTACAATATTTTGATTAGTGGCTTTGGTAAAGATGAGAATGTACCGGCTGCGGTCAAAGTTTTTGAGGCGATAACGAAGCAGGATTTGAAACCAGATGAGTTTACATGTTGTTCACTGATTAATGGGTTGTGCCGCATTGGTGAGCTTGACAAGGCTTGCGATTTACTGGGGGAAATGCGTGGCTTGGGTTTGAAGCCAAATGTTATTACGTACAATGCACTTATTAATGGGTTTTGTAAGAAGAAGATGATGAAGAGAGCGAGAGAGTTATTCGATGACATTGGCAGACAAGGCTTGGAGCCGAGTGTTATTACGTACAATACACTAATTGATGCTTACTCCAAGGATGGGATGATGAAGGAAGCATATGAGGTGTATAATTCAATGTTAGAGAGAGGAGTTTCCCCAAACACTTCGACCTTCAACTGCCGGATTGCTGGTTTGTGCAGACAGGGAAACATGGAGATGGCCATGAAGCTTTTACATGAAATGGAGGTTGATGGCTTGAGAGCTGATCGCATAACATATAACACGTTAATAGATGGATTGTGCAAAGAAGGGAAGTCAAGAAAGGCAGGAACTCTCTTGGATGAGATGTTCAAGAAGCGTTTGAGTCCAAATCACTTGACATACAATTTTTTGATGGATGGATACTGCAGGGAAGGAAACCTCAGTGCAGCTTTGAATCTGAGGGCACTGATGGAGAAAGGAGGGAAGCGACCAAATACTGCAACATATAATGCGTTGATCAAGGGATATTCTAAGAAGGACAAGCTTGAAGATGCGAATGGGCTTCTTAATGAAATGTTGGAGAAGGGGTTGGTCCCAAACCAAACTACTTACGAAATAGTGAAAGAGGAAATGATGAAGAAAGGTTTTGTTCCTGATATAGATTGGCACCTGTATAATATCTCTAGCTCTTAG

Protein Analysis

599

Amino Acids

67.53

Weight (kDa)

9.18

Isoelectric Point (pI)

29.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_3 PF13812 169 - 223 6.5e-06 Pentatricopeptide repeat domain
PPR_long PF17177 177 - 296 7.1e-06 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 181 - 228 1e-10 PPR repeat family
PPR_1 PF12854 212 - 238 1.3e-07 PPR repeat
PPR_2 PF13041 218 - 264 3.4e-15 PPR repeat family
PPR_3 PF13812 239 - 302 2.1e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 246 - 266 3e-08 PPR repeat
PPR_2 PF13041 269 - 302 2.4e-06 PPR repeat family
PPR_long PF17177 281 - 404 1e-05 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 285 - 314 1.5e-06 PPR repeat
PPR_2 PF13041 291 - 337 1.3e-11 PPR repeat family
PPR_3 PF13812 312 - 370 4.8e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 320 - 352 5.2e-09 PPR repeat
PPR PF01535 326 - 356 3.7e-06 PPR repeat
PPR_2 PF13041 330 - 372 6.9e-14 PPR repeat family
PPR_1 PF12854 355 - 385 4.2e-12 PPR repeat
PPR_2 PF13041 358 - 404 2.8e-16 PPR repeat family
PPR PF01535 361 - 390 5e-07 PPR repeat
PPR_long PF17177 375 - 506 2.8e-07 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 382 - 435 3.1e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 390 - 421 2e-10 PPR repeat
PPR_2 PF13041 393 - 435 2e-15 PPR repeat family
PPR PF01535 396 - 426 4.4e-10 PPR repeat
PPR_1 PF12854 424 - 457 4.6e-09 PPR repeat
PPR_2 PF13041 428 - 477 2.3e-16 PPR repeat family
PPR PF01535 431 - 461 1.7e-06 PPR repeat
PPR_3 PF13812 451 - 510 2.8e-12 Pentatricopeptide repeat domain
PPR_1 PF12854 460 - 491 1.9e-10 PPR repeat
PPR_2 PF13041 464 - 512 9.6e-17 PPR repeat family
PPR PF01535 466 - 495 2.5e-07 PPR repeat
PPR_1 PF12854 495 - 526 7.7e-08 PPR repeat
PPR_2 PF13041 501 - 547 4.8e-13 PPR repeat family
PPR_3 PF13812 526 - 574 9.3e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 532 - 561 8.4e-07 PPR repeat
PPR_2 PF13041 533 - 575 1.3e-13 PPR repeat family
PPR PF01535 537 - 566 5.1e-08 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 765
AccB1I GGYRCC 1 cut(s) 1773
AciI CCGC 2 cut(s) 926, 1009
AcoI YGGCCR 2 cut(s) 860, 1344
AcsI RAATTY 5 cut(s) 18, 382, 433, 475, 602
AfiI CCNNNNNNNGG 1 cut(s) 1224
AflIII ACRYGT 3 cut(s) 651, 980, 1407
AjuI GAANNNNNNNTTGG 4 cut(s) 473, 505, 573, 605
AloI GAACNNNNNNTCC 2 cut(s) 1457, 1489
Alw21I GWGCWC 1 cut(s) 150
AoxI GGCC 3 cut(s) 638, 860, 1344
ApeKI GCWGC 4 cut(s) 266, 428, 923, 1553
ApoI RAATTY 5 cut(s) 18, 382, 433, 475, 602
AseI ATTAAT 3 cut(s) 720, 996, 1101
Asp700I GAANNNNTTC 2 cut(s) 257, 504
AspS9I GGNCC 2 cut(s) 639, 1699
AsuHPI GGTGA 6 cut(s) 530, 586, 596, 676, 738, 1028
AvaII GGWCC 1 cut(s) 1699
BaeGI GKGCMC 1 cut(s) 1573
BaeI ACNNNNGTAYC 4 cut(s) 54, 87, 218, 251
BalI TGGCCA 1 cut(s) 1346
BanI GGYRCC 1 cut(s) 1773
BanII GRGCYC 1 cut(s) 150
BbsI GAAGAC 1 cut(s) 488
Bbv12I GWGCWC 1 cut(s) 150
BbvI GCAGC 4 cut(s) 253, 415, 910, 1565
BciVI GTATCC 1 cut(s) 1520
BclI TGATCA 1 cut(s) 1623
BfaI CTAG 2 cut(s) 278, 1791
BfmI CTRYAG 1 cut(s) 1531
BfuI GTATCC 1 cut(s) 1520
BisI GCNGC 5 cut(s) 267, 429, 924, 1009, 1554
BlsI GCNGC 5 cut(s) 268, 430, 925, 1010, 1555
BmcAI AGTACT 1 cut(s) 222
Bme18I GGWCC 1 cut(s) 1699
BmgT120I GGNCC 2 cut(s) 639, 1699
BmiI GGNNCC 3 cut(s) 1176, 1701, 1775
BmrI ACTGGG 2 cut(s) 212, 1052
BmsI GCATC 4 cut(s) 13, 814, 1198, 1648
BmuI ACTGGG 2 cut(s) 212, 1052
BpiI GAAGAC 1 cut(s) 488
BpmI CTGGAG 1 cut(s) 228
BpuEI CTTGAG 1 cut(s) 1402
Bsa29I ATCGAT 1 cut(s) 777
BsaAI YACGTR 2 cut(s) 1086, 1191
BsaJI CCNNGG 1 cut(s) 1218
BsaXI ACNNNNNCTCC 2 cut(s) 1679, 1709
Bsc4I CCNNNNNNNGG 1 cut(s) 1224
Bse118I RCCGGY 1 cut(s) 919
Bse1I ACTGG 3 cut(s) 114, 218, 1047
Bse3DI GCAATG 1 cut(s) 373
BseCI ATCGAT 1 cut(s) 777
BseDI CCNNGG 1 cut(s) 1218
BseGI GGATG 6 cut(s) 4, 715, 1228, 1233, 1471, 1528
BseLI CCNNNNNNNGG 1 cut(s) 1224
BseMI GCAATG 1 cut(s) 373
BseMII CTCAG 4 cut(s) 163, 244, 1556, 1561
BseNI ACTGG 3 cut(s) 114, 218, 1047
BseRI GAGGAG 3 cut(s) 643, 713, 1287
BseSI GKGCMC 1 cut(s) 1573
BseXI GCAGC 4 cut(s) 253, 415, 910, 1565
BsgI GTGCAG 3 cut(s) 252, 1342, 1572
BshFI GGCC 3 cut(s) 640, 862, 1346
BshNI GGYRCC 1 cut(s) 1773
BshVI ATCGAT 1 cut(s) 777
BsiHKAI GWGCWC 1 cut(s) 150
BsiSI CCGG 2 cut(s) 920, 1306
BslFI GGGAC 1 cut(s) 1685
BslI CCNNNNNNNGG 1 cut(s) 1224
BsmFI GGGAC 1 cut(s) 1685
BsnI GGCC 3 cut(s) 640, 862, 1346
Bsp1286I GDGCHC 2 cut(s) 150, 1573
Bsp1407I TGTACA 2 cut(s) 648, 813
Bsp143I GATC 3 cut(s) 774, 1390, 1623
BspACI CCGC 2 cut(s) 926, 1009
BspANI GGCC 3 cut(s) 640, 862, 1346
BspCNI CTCAG 4 cut(s) 162, 243, 1557, 1560
BspDI ATCGAT 1 cut(s) 777
BspLI GGNNCC 3 cut(s) 1176, 1701, 1775
BspMAI CTGCAG 1 cut(s) 1535
BspT107I GGYRCC 1 cut(s) 1773
BsrDI GCAATG 1 cut(s) 373
BsrFI RCCGGY 1 cut(s) 919
BsrGI TGTACA 2 cut(s) 648, 813
BsrI ACTGG 3 cut(s) 114, 218, 1047
BssAI RCCGGY 1 cut(s) 919
BssECI CCNNGG 1 cut(s) 1218
BssMI GATC 3 cut(s) 774, 1390, 1623
BssT1I CCWWGG 1 cut(s) 1218
Bst6I CTCTTC 1 cut(s) 1124
BstAUI TGTACA 2 cut(s) 648, 813
BstBAI YACGTR 2 cut(s) 1086, 1191
BstC8I GCNNGC 1 cut(s) 1033
BstDEI CTNAG 6 cut(s) 149, 230, 1547, 1565, 1638, 1797
BstF5I GGATG 6 cut(s) 4, 715, 1228, 1233, 1471, 1528
BstKTI GATC 3 cut(s) 777, 1393, 1626
BstMBI GATC 3 cut(s) 774, 1390, 1623
BstMWI GCNNNNNNNGC 1 cut(s) 1167
BstNSI RCATGY 1 cut(s) 984
BstSFI CTRYAG 1 cut(s) 1531
BstSLI GKGCMC 1 cut(s) 1573
BstSNI TACGTA 2 cut(s) 1086, 1191
BstV1I GCAGC 4 cut(s) 253, 415, 910, 1565
BstV2I GAAGAC 1 cut(s) 488
Bsu15I ATCGAT 1 cut(s) 777
BsuI GTATCC 1 cut(s) 1520
BsuRI GGCC 3 cut(s) 640, 862, 1346
BsuTUI ATCGAT 1 cut(s) 777
BtsCI GGATG 6 cut(s) 4, 715, 1228, 1233, 1471, 1528
BtsIMutI CAGTG 4 cut(s) 107, 989, 1555, 1571
Cac8I GCNNGC 1 cut(s) 1033
Cfr10I RCCGGY 1 cut(s) 919
Cfr13I GGNCC 2 cut(s) 639, 1699
ClaI ATCGAT 1 cut(s) 777
CspCI CAANNNNNGTGG 2 cut(s) 778, 813
CviAII CATG 6 cut(s) 350, 727, 981, 1336, 1348, 1361
DdeI CTNAG 6 cut(s) 149, 230, 1547, 1565, 1638, 1797
DpnI GATC 3 cut(s) 776, 1392, 1625
DpnII GATC 3 cut(s) 774, 1390, 1623
EaeI YGGCCR 2 cut(s) 860, 1344
Eam1104I CTCTTC 1 cut(s) 1124
EarI CTCTTC 1 cut(s) 1124
Ecl136II GAGCTC 1 cut(s) 148
Eco105I TACGTA 2 cut(s) 1086, 1191
Eco130I CCWWGG 1 cut(s) 1218
Eco24I GRGCYC 1 cut(s) 150
Eco32I GATATC 1 cut(s) 744
Eco47I GGWCC 1 cut(s) 1699
Eco53kI GAGCTC 1 cut(s) 148
EcoICRI GAGCTC 1 cut(s) 148
EcoRV GATATC 1 cut(s) 744
EcoT14I CCWWGG 1 cut(s) 1218
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 150
ErhI CCWWGG 1 cut(s) 1218
FaeI CATG 6 cut(s) 353, 730, 984, 1339, 1351, 1364
FaqI GGGAC 1 cut(s) 1685
FatI CATG 6 cut(s) 349, 726, 980, 1335, 1347, 1360
FauNDI CATATG 2 cut(s) 466, 1243
FbaI TGATCA 1 cut(s) 1623
Fnu4HI GCNGC 5 cut(s) 267, 429, 924, 1009, 1554
FokI GGATG 5 cut(s) 722, 1235, 1240, 1478, 1535
FriOI GRGCYC 1 cut(s) 150
Fsp4HI GCNGC 5 cut(s) 267, 429, 924, 1009, 1554
FspBI CTAG 2 cut(s) 278, 1791
GluI GCNGC 5 cut(s) 267, 429, 924, 1009, 1554
GsuI CTGGAG 1 cut(s) 228
HaeIII GGCC 3 cut(s) 640, 862, 1346
HapII CCGG 2 cut(s) 920, 1306
Hin1II CATG 6 cut(s) 353, 730, 984, 1339, 1351, 1364
HincII GTYRAC 1 cut(s) 448
HindII GTYRAC 1 cut(s) 448
HindIII AAGCTT 2 cut(s) 1352, 1649
HinfI GANTC 7 cut(s) 49, 100, 167, 203, 294, 1489, 1561
HpaI GTTAAC 1 cut(s) 448
HpaII CCGG 2 cut(s) 920, 1306
HphI GGTGA 6 cut(s) 530, 586, 596, 676, 738, 1028
Hpy166II GTNNAC 7 cut(s) 419, 448, 610, 651, 873, 978, 989
Hpy188I TCNGA 7 cut(s) 91, 118, 145, 152, 172, 510, 1566
Hpy188III TCNNGA 5 cut(s) 207, 278, 1443, 1477, 1760
Hpy8I GTNNAC 7 cut(s) 419, 448, 610, 651, 873, 978, 989
HpyCH4IV ACGT 4 cut(s) 653, 1085, 1190, 1409
HpyF10VI GCNNNNNNNGC 1 cut(s) 1167
HpyF3I CTNAG 6 cut(s) 149, 230, 1547, 1565, 1638, 1797
HpySE526I ACGT 4 cut(s) 653, 1085, 1190, 1409
Hsp92II CATG 6 cut(s) 353, 730, 984, 1339, 1351, 1364
Ksp22I TGATCA 1 cut(s) 1623
KspAI GTTAAC 1 cut(s) 448
Kzo9I GATC 3 cut(s) 774, 1390, 1623
LmnI GCTCC 2 cut(s) 209, 1174
Lsp1109I GCAGC 4 cut(s) 253, 415, 910, 1565
LweI GCATC 4 cut(s) 13, 814, 1198, 1648
MaeI CTAG 2 cut(s) 278, 1791
MaeII ACGT 4 cut(s) 653, 1085, 1190, 1409
MaeIII GTNAC 1 cut(s) 551
MalI GATC 3 cut(s) 776, 1392, 1625
MboI GATC 3 cut(s) 774, 1390, 1623
MboII GAAGA 8 cut(s) 95, 493, 820, 1129, 1132, 1141, 1667, 1756
MhlI GDGCHC 2 cut(s) 150, 1573
MlsI TGGCCA 1 cut(s) 1346
MluNI TGGCCA 1 cut(s) 1346
MlyI GAGTC 4 cut(s) 43, 161, 288, 1498
MmeI TCCRAC 1 cut(s) 1665
Mox20I TGGCCA 1 cut(s) 1346
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 2 cut(s) 257, 504
MscI TGGCCA 1 cut(s) 1346
MslI CAYNNNNRTG 2 cut(s) 492, 1340
Msp20I TGGCCA 1 cut(s) 1346
MspI CCGG 2 cut(s) 920, 1306
MwoI GCNNNNNNNGC 1 cut(s) 1167
NdeI CATATG 2 cut(s) 466, 1243
NdeII GATC 3 cut(s) 774, 1390, 1623
NlaIII CATG 6 cut(s) 353, 730, 984, 1339, 1351, 1364
NlaIV GGNNCC 3 cut(s) 1176, 1701, 1775
NmeAIII GCCGAG 1 cut(s) 1203
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 984
PciI ACATGT 1 cut(s) 980
PdmI GAANNNNTTC 2 cut(s) 257, 504
PfeI GAWTC 3 cut(s) 100, 203, 1561
PkrI GCNGC 5 cut(s) 268, 430, 925, 1010, 1555
PleI GAGTC 4 cut(s) 43, 161, 288, 1497
PpsI GAGTC 4 cut(s) 43, 161, 288, 1497
Ppu21I YACGTR 2 cut(s) 1086, 1191
PscI ACATGT 1 cut(s) 980
PshBI ATTAAT 3 cut(s) 720, 996, 1101
PsiI TTATAA 1 cut(s) 765
Psp124BI GAGCTC 1 cut(s) 150
PspN4I GGNNCC 3 cut(s) 1176, 1701, 1775
PspPI GGNCC 2 cut(s) 639, 1699
PstI CTGCAG 1 cut(s) 1535
RseI CAYNNNNRTG 2 cut(s) 492, 1340
SacI GAGCTC 1 cut(s) 150
SatI GCNGC 5 cut(s) 267, 429, 924, 1009, 1554
Sau3AI GATC 3 cut(s) 774, 1390, 1623
Sau96I GGNCC 2 cut(s) 639, 1699
ScaI AGTACT 1 cut(s) 222
SchI GAGTC 4 cut(s) 43, 161, 288, 1498
SduI GDGCHC 2 cut(s) 150, 1573
SfaNI GCATC 4 cut(s) 13, 814, 1198, 1648
SfcI CTRYAG 1 cut(s) 1531
SinI GGWCC 1 cut(s) 1699
SmiMI CAYNNNNRTG 2 cut(s) 492, 1340
SmlI CTYRAG 1 cut(s) 1381
SmoI CTYRAG 1 cut(s) 1381
SnaBI TACGTA 2 cut(s) 1086, 1191
SsiI CCGC 2 cut(s) 926, 1009
SspI AATATT 2 cut(s) 856, 883
SspMI CTAG 2 cut(s) 278, 1791
SstI GAGCTC 1 cut(s) 150
StyI CCWWGG 1 cut(s) 1218
TaiI ACGT 4 cut(s) 656, 1088, 1193, 1412
TaqI TCGA 3 cut(s) 777, 1149, 1292
TatI WGTACW 4 cut(s) 220, 648, 813, 872
TauI GCSGC 1 cut(s) 1011
TfiI GAWTC 3 cut(s) 100, 203, 1561
TscAI CASTG 4 cut(s) 114, 996, 1555, 1578
TseI GCWGC 4 cut(s) 266, 428, 923, 1553
TspRI CASTG 4 cut(s) 114, 996, 1555, 1578
VpaK11BI GGWCC 1 cut(s) 1699
VspI ATTAAT 3 cut(s) 720, 996, 1101
XapI RAATTY 5 cut(s) 18, 382, 433, 475, 602
XbaI TCTAGA 1 cut(s) 277
XceI RCATGY 1 cut(s) 984
XmnI GAANNNNTTC 2 cut(s) 257, 504
XspI CTAG 2 cut(s) 278, 1791
ZrmI AGTACT 1 cut(s) 222
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.