Rw2G010250

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0008008
Physical Location & Seq
Reverse (-)
10355691 .. 10357520
1830 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G010250.1

Sequence Viewer

Length: 1830 bp
ATGCTAAAGCTCCTCTCCTCCTCCTCATCATGCCTCCACGCTCGAGCCCACATTCAGCAGCACCCCCAAATCAACAGAATCAAACTCTGTTCCACAATCTCCATCTCCGAAAATCCAAGCTCACAGTCCTTCAATCTTCCATTAGTTTCAGACCTCATAGCAAAGCAACACTGGTCAGAGCTCAAAACCCATCTCAAAGACTCAACCTTTATCACAGTTCTTCACCAACTGATCGATTCTGGAGCTGACCCAGAGCTCACACTCAGGTACTTCAATTGGGCCCAGAAGGGTCAAAATGTTCCACACAATATTGAAATCACTTGTAGACTATTGCAGTCACTTTCAAATGCAAAGAAGTACCCAAAGATGAGAGCTTTCTTAGATGAATTTGTTAGAAGGAATGAGAAGCATTCAGTTTCTTCAATTTTTCATATGCTTTCAATGTATAGCAATCACTTTTGTGCTAATTCCATTATAGTTGATATGCTGGTGTTGGCTTATGTTAAGAATTCGAAGACCAATTCGGGTTTTGAGGCGTTTCAGAGAGCTGGTGATTATGGGTTTAGGCTGTCAAAACTGTCTTGTAATGCTTTGTTGAGTGCTTTGGTGAAGGAAAATGAAATTGGGTATGTTGAGTTTGTGTACAAGGAGATGTTTAGGAGGAGGATAGAGGCGGATTTGTATAGTTTCAATATTGTGATTAATGGGTTGTGTAAAGTTGGGAAATTGAACAAGGCAAAGGATGTTATGGATGATATGAAGGCTTGGGGGGTTGCACCAAATGTGGTTACTTATAATACTGTCATTGGTGGGTATTGCAAATTGGGTGGGTTGGGGAAAATGCATAAGGCTGATGCCATATTGAAGGAGATGGTGGCGAACAATATTTGTCCGAGTGTGATCACGTTCAATATTTTGATTAATGGGTTCTGTAAAGATGAGAATGTAGCAGCTGCGTTAAAGGTGTTTGAGGAAATGAGGAGGCAGGGCTTGAAACCTAATTTGGTTACTTATAATTCACTGATTAATGGGTTATGCTGTGATGGGAAGCTTGAGGAGGCTTGTGGATTGCGGGACGAAATGCTTGGATTGGGTTTGAAGCCAGAGATAGCAACCTACAATGCACTTATAAATGGGTTTTGCAAGAAGAAAATGCTGAAGGAAGCTAGAGAGCTGTTTGATAGCATTTGTGAGCGAGGCTTGGTAACCAATGTTATAACATTTAATTCACTAATTGATGCTTATTGTAAGAATGAGATGAAGGAGGAAGCATATGAATTGCATAGCATGATGATAGAGAGAGGGGTTTTACCAAACACGTCAACATTTAACTGCTTGATTCATGGTTCTCTTAGACAGGGAAACATAGAAACAGCCAGGAAATTCTTACAACAAATGGAGGCTGAGGGCTTGAAAGCTGATTGCATAACATATGATATACTGATAAATGCATTATGTAAAGATGGGGAGCCAAGAAAGGCAGAAAGACTCCTGAATGAGATGTTCACTAAGGGTCTGAGCCCAAGGCACGTGACATACAATACTTTGATGGATGGCTACTGCAGGGAAGGAAACCTGAAGGCAGCTTTGAACTTGAGGACTCGGATGGAGAAAGAAGGGAAACGAGCAAATATAGCAACATACAATTTGCTGATCAAGGGATATTGTATGCAGGACAAGCTTGTAGATGCGAATGTACTTCTAAATGAAATGTTGGAGAAAGGTTTGATCCCAAATCGAACTACTTATGAAATAGTTAAAGTGGAAATGATGGACAAAGGTTTTGTTCCTGATATAGATGGACACCTGCATAATATCTCTAGCTCTTAA

Protein Analysis

609

Amino Acids

68.75

Weight (kDa)

8.58

Isoelectric Point (pI)

38.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 194 - 239 7e-09 PPR repeat family
PPR_3 PF13812 215 - 272 2.1e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 223 - 254 5.4e-09 PPR repeat
PPR_2 PF13041 226 - 274 4.3e-18 PPR repeat family
PPR PF01535 228 - 258 1.4e-06 PPR repeat
PPR_1 PF12854 257 - 291 2.8e-07 PPR repeat
PPR_2 PF13041 279 - 312 1.3e-08 PPR repeat family
PPR_long PF17177 284 - 378 3.4e-07 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 287 - 343 5.7e-15 Pentatricopeptide repeat domain
PPR_1 PF12854 295 - 327 6.8e-13 PPR repeat
PPR_2 PF13041 298 - 346 3.7e-20 PPR repeat family
TPR_24 PF23276 301 - 395 1.8e-06 Fungal tetratrico peptide repeats
PPR PF01535 301 - 331 1.2e-07 PPR repeat
PPR_1 PF12854 330 - 361 4.1e-14 PPR repeat
PPR_2 PF13041 336 - 382 2.5e-14 PPR repeat family
PPR PF01535 336 - 366 2.3e-08 PPR repeat
PPR_3 PF13812 359 - 418 4.2e-09 Pentatricopeptide repeat domain
PPR_1 PF12854 365 - 394 5.1e-10 PPR repeat
PPR_2 PF13041 368 - 409 2.1e-10 PPR repeat family
PPR PF01535 372 - 401 4.7e-08 PPR repeat
TPR_24 PF23276 391 - 494 2.1e-07 Fungal tetratrico peptide repeats
PPR_1 PF12854 399 - 431 6.7e-08 PPR repeat
PPR_2 PF13041 404 - 449 5.5e-14 PPR repeat family
PPR PF01535 406 - 436 1.7e-08 PPR repeat
PPR_3 PF13812 431 - 485 1.8e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 434 - 467 1.4e-06 PPR repeat
PPR_2 PF13041 438 - 487 5.8e-14 PPR repeat family
PPR_long PF17177 456 - 541 7.3e-07 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 470 - 501 1.5e-10 PPR repeat
PPR PF01535 476 - 506 6.7e-07 PPR repeat
PPR_2 PF13041 476 - 508 3.9e-07 PPR repeat family
PPR_3 PF13812 496 - 555 4.7e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 504 - 537 1.6e-10 PPR repeat
PPR_2 PF13041 510 - 556 8.5e-15 PPR repeat family
PPR PF01535 511 - 540 1.7e-07 PPR repeat
PPR_3 PF13812 536 - 584 6.2e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 543 - 571 7.4e-06 PPR repeat
PPR_2 PF13041 544 - 585 3.7e-11 PPR repeat family
PPR PF01535 547 - 576 7.9e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 795, 1014, 1130, 1217
AarI CACCTGC 1 cut(s) 1815
Acc36I ACCTGC 1 cut(s) 1815
AccI GTMKAC 1 cut(s) 325
AciI CCGC 2 cut(s) 674, 1072
AclWI GGATC 1 cut(s) 1723
AcsI RAATTY 3 cut(s) 386, 508, 1382
AcuI CTGAAG 2 cut(s) 1178, 1598
AcvI CACGTG 1 cut(s) 1531
AfaI GTAC 4 cut(s) 269, 359, 644, 1698
AflIII ACRYGT 1 cut(s) 1317
AjiI CACGTC 1 cut(s) 1320
AjnI CCWGG 1 cut(s) 1376
AjuI GAANNNNNNNTTGG 4 cut(s) 606, 638, 986, 1018
AleI CACNNNNGTG 1 cut(s) 459
Alw21I GWGCWC 2 cut(s) 183, 258
AlwI GGATC 1 cut(s) 1723
Ama87I CYCGRG 1 cut(s) 42
AoxI GGCC 1 cut(s) 279
ApaI GGGCCC 1 cut(s) 283
ApeKI GCWGC 4 cut(s) 58, 950, 953, 1583
ApoI RAATTY 3 cut(s) 386, 508, 1382
ArsI GACNNNNNNTTYG 2 cut(s) 1766, 1798
AseI ATTAAT 3 cut(s) 702, 921, 1026
AspS9I GGNCC 2 cut(s) 279, 280
AsuHPI GGTGA 3 cut(s) 215, 563, 619
AsuII TTCGAA 1 cut(s) 512
AvaI CYCGRG 1 cut(s) 42
BaeGI GKGCMC 1 cut(s) 283
BanII GRGCYC 5 cut(s) 49, 183, 258, 283, 1523
BbrPI CACGTG 1 cut(s) 1531
BbsI GAAGAC 1 cut(s) 521
Bbv12I GWGCWC 2 cut(s) 183, 258
BbvCI CCTCAGC 1 cut(s) 1404
BbvI GCAGC 4 cut(s) 70, 940, 962, 1595
BciT130I CCWGG 1 cut(s) 1378
BclI TGATCA 2 cut(s) 900, 1653
BfaI CTAG 2 cut(s) 1167, 1821
BfmI CTRYAG 1 cut(s) 1561
BfuAI ACCTGC 1 cut(s) 1815
BisI GCNGC 4 cut(s) 59, 951, 954, 1584
BlsI GCNGC 4 cut(s) 60, 952, 955, 1585
Bme1390I CCNGG 1 cut(s) 1378
BmeT110I CYCGRG 1 cut(s) 42
BmgBI CACGTC 1 cut(s) 1320
BmgT120I GGNCC 2 cut(s) 279, 280
BmiI GGNNCC 2 cut(s) 281, 1470
BmrFI CCNGG 1 cut(s) 1378
BmsI GCATC 3 cut(s) 844, 1228, 1678
BpiI GAAGAC 1 cut(s) 521
BpmI CTGGAG 1 cut(s) 261
Bpu10I CCTNAGC 1 cut(s) 1404
Bpu14I TTCGAA 1 cut(s) 512
BpuEI CTTGAG 2 cut(s) 1073, 1615
Bsa29I ATCGAT 1 cut(s) 234
BsaAI YACGTR 1 cut(s) 1531
BsaJI CCNNGG 1 cut(s) 1523
Bse1I ACTGG 1 cut(s) 176
BseBI CCWGG 1 cut(s) 1378
BseCI ATCGAT 1 cut(s) 234
BseDI CCNNGG 1 cut(s) 1523
BseGI GGATG 4 cut(s) 748, 757, 1558, 1611
BseMII CTCAG 3 cut(s) 277, 1395, 1508
BseNI ACTGG 1 cut(s) 176
BseRI GAGGAG 6 cut(s) 7, 10, 13, 676, 994, 1070
BseSI GKGCMC 1 cut(s) 283
BseXI GCAGC 4 cut(s) 70, 940, 962, 1595
BshFI GGCC 1 cut(s) 281
BshVI ATCGAT 1 cut(s) 234
BsiHKAI GWGCWC 2 cut(s) 183, 258
BsiHKCI CYCGRG 1 cut(s) 42
BslFI GGGAC 1 cut(s) 1088
BsmFI GGGAC 1 cut(s) 1088
BsmI GAATGC 1 cut(s) 409
BsnI GGCC 1 cut(s) 281
BsoBI CYCGRG 1 cut(s) 42
Bsp119I TTCGAA 1 cut(s) 512
Bsp120I GGGCCC 1 cut(s) 279
Bsp1286I GDGCHC 5 cut(s) 49, 183, 258, 283, 1523
Bsp1407I TGTACA 1 cut(s) 642
Bsp143I GATC 4 cut(s) 231, 900, 1653, 1728
BspACI CCGC 2 cut(s) 674, 1072
BspANI GGCC 1 cut(s) 281
BspCNI CTCAG 3 cut(s) 276, 1396, 1509
BspDI ATCGAT 1 cut(s) 234
BspLI GGNNCC 2 cut(s) 281, 1470
BspMAI CTGCAG 1 cut(s) 1565
BspMI ACCTGC 1 cut(s) 1815
BspPI GGATC 1 cut(s) 1723
BspT104I TTCGAA 1 cut(s) 512
BsrGI TGTACA 1 cut(s) 642
BsrI ACTGG 1 cut(s) 176
BssECI CCNNGG 1 cut(s) 1523
BssMI GATC 4 cut(s) 231, 900, 1653, 1728
BssT1I CCWWGG 1 cut(s) 1523
Bst2UI CCWGG 1 cut(s) 1378
Bst4CI ACNGT 4 cut(s) 126, 217, 579, 802
BstAUI TGTACA 1 cut(s) 642
BstBAI YACGTR 1 cut(s) 1531
BstBI TTCGAA 1 cut(s) 512
BstDEI CTNAG 6 cut(s) 263, 379, 1352, 1404, 1509, 1517
BstEII GGTNACC 1 cut(s) 1204
BstF5I GGATG 4 cut(s) 748, 757, 1558, 1611
BstKTI GATC 4 cut(s) 234, 903, 1656, 1731
BstMBI GATC 4 cut(s) 231, 900, 1653, 1728
BstMWI GCNNNNNNNGC 2 cut(s) 1634, 1678
BstNI CCWGG 1 cut(s) 1378
BstPI GGTNACC 1 cut(s) 1204
BstSCI CCNGG 1 cut(s) 1376
BstSFI CTRYAG 1 cut(s) 1561
BstSLI GKGCMC 1 cut(s) 283
BstV1I GCAGC 4 cut(s) 70, 940, 962, 1595
BstV2I GAAGAC 1 cut(s) 521
Bsu15I ATCGAT 1 cut(s) 234
BsuRI GGCC 1 cut(s) 281
BsuTUI ATCGAT 1 cut(s) 234
BtrI CACGTC 1 cut(s) 1320
BtsCI GGATG 4 cut(s) 748, 757, 1558, 1611
BtsIMutI CAGTG 2 cut(s) 169, 1019
BveI ACCTGC 1 cut(s) 1815
Cfr13I GGNCC 2 cut(s) 279, 280
ClaI ATCGAT 1 cut(s) 234
Csp6I GTAC 4 cut(s) 268, 358, 643, 1697
CspCI CAANNNNNGTGG 2 cut(s) 808, 843
CviAII CATG 3 cut(s) 30, 1288, 1343
CviQI GTAC 4 cut(s) 268, 358, 643, 1697
DdeI CTNAG 6 cut(s) 263, 379, 1352, 1404, 1509, 1517
DpnI GATC 4 cut(s) 233, 902, 1655, 1730
DpnII GATC 4 cut(s) 231, 900, 1653, 1728
EciI GGCGGA 1 cut(s) 689
Ecl136II GAGCTC 2 cut(s) 181, 256
Eco130I CCWWGG 1 cut(s) 1523
Eco24I GRGCYC 5 cut(s) 49, 183, 258, 283, 1523
Eco53kI GAGCTC 2 cut(s) 181, 256
Eco57I CTGAAG 2 cut(s) 1178, 1598
Eco72I CACGTG 1 cut(s) 1531
Eco88I CYCGRG 1 cut(s) 42
Eco91I GGTNACC 1 cut(s) 1204
EcoICRI GAGCTC 2 cut(s) 181, 256
EcoO65I GGTNACC 1 cut(s) 1204
EcoRI GAATTC 1 cut(s) 508
EcoRII CCWGG 1 cut(s) 1376
EcoT14I CCWWGG 1 cut(s) 1523
EcoT22I ATGCAT 2 cut(s) 846, 1453
EcoT38I GRGCYC 5 cut(s) 49, 183, 258, 283, 1523
ErhI CCWWGG 1 cut(s) 1523
FaeI CATG 3 cut(s) 33, 1291, 1346
FaqI GGGAC 1 cut(s) 1088
FatI CATG 3 cut(s) 29, 1287, 1342
FauI CCCGC 1 cut(s) 1065
FauNDI CATATG 3 cut(s) 432, 1273, 1432
FbaI TGATCA 2 cut(s) 900, 1653
FblI GTMKAC 1 cut(s) 325
Fnu4HI GCNGC 4 cut(s) 59, 951, 954, 1584
FokI GGATG 4 cut(s) 755, 764, 1565, 1618
FriOI GRGCYC 5 cut(s) 49, 183, 258, 283, 1523
Fsp4HI GCNGC 4 cut(s) 59, 951, 954, 1584
FspBI CTAG 2 cut(s) 1167, 1821
GluI GCNGC 4 cut(s) 59, 951, 954, 1584
GsuI CTGGAG 1 cut(s) 261
HaeIII GGCC 1 cut(s) 281
Hin1II CATG 3 cut(s) 33, 1291, 1346
HincII GTYRAC 1 cut(s) 1323
HindII GTYRAC 1 cut(s) 1323
HindIII AAGCTT 2 cut(s) 1049, 1679
HinfI GANTC 6 cut(s) 78, 200, 236, 1339, 1488, 1600
HphI GGTGA 3 cut(s) 215, 563, 619
Hpy166II GTNNAC 4 cut(s) 326, 643, 1323, 1506
Hpy188I TCNGA 7 cut(s) 109, 151, 178, 543, 894, 1518, 1605
Hpy188III TCNNGA 3 cut(s) 240, 1492, 1790
Hpy8I GTNNAC 4 cut(s) 326, 643, 1323, 1506
HpyCH4III ACNGT 4 cut(s) 126, 217, 579, 802
HpyCH4IV ACGT 3 cut(s) 905, 1319, 1530
HpyF10VI GCNNNNNNNGC 2 cut(s) 1634, 1678
HpyF3I CTNAG 6 cut(s) 263, 379, 1352, 1404, 1509, 1517
HpySE526I ACGT 3 cut(s) 905, 1319, 1530
Hsp92II CATG 3 cut(s) 33, 1291, 1346
Ksp22I TGATCA 2 cut(s) 900, 1653
Kzo9I GATC 4 cut(s) 231, 900, 1653, 1728
LmnI GCTCC 3 cut(s) 15, 242, 1468
Lsp1109I GCAGC 4 cut(s) 70, 940, 962, 1595
LweI GCATC 3 cut(s) 844, 1228, 1678
MaeI CTAG 2 cut(s) 1167, 1821
MaeII ACGT 3 cut(s) 905, 1319, 1530
MaeIII GTNAC 5 cut(s) 336, 787, 1006, 1204, 1531
MalI GATC 4 cut(s) 233, 902, 1655, 1730
MboI GATC 4 cut(s) 231, 900, 1653, 1728
MboII GAAGA 5 cut(s) 128, 212, 411, 526, 1159
MfeI CAATTG 1 cut(s) 274
MhlI GDGCHC 5 cut(s) 49, 183, 258, 283, 1523
MlyI GAGTC 3 cut(s) 194, 1482, 1594
MmeI TCCRAC 1 cut(s) 1695
Mph1103I ATGCAT 2 cut(s) 846, 1453
MseI TTAA 9 cut(s) 504, 702, 921, 959, 1026, 1224, 1329, 1758, 1828
MslI CAYNNNNRTG 1 cut(s) 459
MspA1I CMGCKG 1 cut(s) 953
MspR9I CCNGG 1 cut(s) 1378
MunI CAATTG 1 cut(s) 274
Mva1269I GAATGC 1 cut(s) 409
MvaI CCWGG 1 cut(s) 1378
MwoI GCNNNNNNNGC 2 cut(s) 1634, 1678
NdeI CATATG 3 cut(s) 432, 1273, 1432
NdeII GATC 4 cut(s) 231, 900, 1653, 1728
NlaIII CATG 3 cut(s) 33, 1291, 1346
NlaIV GGNNCC 2 cut(s) 281, 1470
NmuCI GTSAC 2 cut(s) 336, 1531
NsiI ATGCAT 2 cut(s) 846, 1453
NspV TTCGAA 1 cut(s) 512
OliI CACNNNNGTG 1 cut(s) 459
PaeR7I CTCGAG 1 cut(s) 42
PaqCI CACCTGC 1 cut(s) 1815
PctI GAATGC 1 cut(s) 409
PfeI GAWTC 3 cut(s) 78, 236, 1339
PkrI GCNGC 4 cut(s) 60, 952, 955, 1585
PleI GAGTC 3 cut(s) 194, 1482, 1594
PmaCI CACGTG 1 cut(s) 1531
PmlI CACGTG 1 cut(s) 1531
PpsI GAGTC 3 cut(s) 194, 1482, 1594
Ppu21I YACGTR 1 cut(s) 1531
PshBI ATTAAT 3 cut(s) 702, 921, 1026
PsiI TTATAA 4 cut(s) 795, 1014, 1130, 1217
Psp124BI GAGCTC 2 cut(s) 183, 258
Psp6I CCWGG 1 cut(s) 1376
PspCI CACGTG 1 cut(s) 1531
PspEI GGTNACC 1 cut(s) 1204
PspGI CCWGG 1 cut(s) 1376
PspN4I GGNNCC 2 cut(s) 281, 1470
PspOMI GGGCCC 1 cut(s) 279
PspPI GGNCC 2 cut(s) 279, 280
PspXI VCTCGAGB 1 cut(s) 42
PstI CTGCAG 1 cut(s) 1565
PvuII CAGCTG 1 cut(s) 953
RsaI GTAC 4 cut(s) 269, 359, 644, 1698
RsaNI GTAC 4 cut(s) 268, 358, 643, 1697
RseI CAYNNNNRTG 1 cut(s) 459
SacI GAGCTC 2 cut(s) 183, 258
SaqAI TTAA 9 cut(s) 504, 702, 921, 959, 1026, 1224, 1329, 1758, 1828
SatI GCNGC 4 cut(s) 59, 951, 954, 1584
Sau3AI GATC 4 cut(s) 231, 900, 1653, 1728
Sau96I GGNCC 2 cut(s) 279, 280
SchI GAGTC 3 cut(s) 194, 1482, 1594
ScrFI CCNGG 1 cut(s) 1378
SduI GDGCHC 5 cut(s) 49, 183, 258, 283, 1523
SfaNI GCATC 3 cut(s) 844, 1228, 1678
SfcI CTRYAG 1 cut(s) 1561
Sfr274I CTCGAG 1 cut(s) 42
SfuI TTCGAA 1 cut(s) 512
SlaI CTCGAG 1 cut(s) 42
SmiMI CAYNNNNRTG 1 cut(s) 459
SmlI CTYRAG 3 cut(s) 42, 1052, 1594
SmoI CTYRAG 3 cut(s) 42, 1052, 1594
SsiI CCGC 2 cut(s) 674, 1072
SspI AATATT 4 cut(s) 310, 694, 886, 913
SspMI CTAG 2 cut(s) 1167, 1821
SstI GAGCTC 2 cut(s) 183, 258
StyD4I CCNGG 1 cut(s) 1376
StyI CCWWGG 1 cut(s) 1523
TaaI ACNGT 4 cut(s) 126, 217, 579, 802
TaiI ACGT 3 cut(s) 908, 1322, 1533
TaqI TCGA 4 cut(s) 43, 234, 512, 1738
TatI WGTACW 2 cut(s) 642, 1696
TfiI GAWTC 3 cut(s) 78, 236, 1339
Tru1I TTAA 9 cut(s) 504, 702, 921, 959, 1026, 1224, 1329, 1758, 1828
Tru9I TTAA 9 cut(s) 504, 702, 921, 959, 1026, 1224, 1329, 1758, 1828
TscAI CASTG 2 cut(s) 176, 1026
TseFI GTSAC 2 cut(s) 336, 1531
TseI GCWGC 4 cut(s) 58, 950, 953, 1583
Tsp45I GTSAC 2 cut(s) 336, 1531
TspDTI ATGAA 9 cut(s) 399, 419, 633, 773, 1274, 1290, 1331, 1722, 1764
TspRI CASTG 2 cut(s) 176, 1026
VspI ATTAAT 3 cut(s) 702, 921, 1026
XapI RAATTY 3 cut(s) 386, 508, 1382
XhoI CTCGAG 1 cut(s) 42
XmiI GTMKAC 1 cut(s) 325
XspI CTAG 2 cut(s) 1167, 1821
Zsp2I ATGCAT 2 cut(s) 846, 1453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.