Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0008791

Physical Location & Seq

Genomic Coordinates Forward (+)

5234759 .. 5240088

Gene Structure 5330 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g08810.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2319 bp

ATGACTTCTATCATTGGAGTTTCGTCTATATATTACCAGACTCCATGTGAGCTGTACAAAAGAGCTGCCGCTCCTCTAGCAGCCGCCACAACGGCGACGACGACGGCGACGAGCAGTAGGAGCGGCGGAGTTTCGTTGGCGTTTCCGGAGAGGCAGAGGGGGCACTTCAACACGGTGTTGAGAGCGAGGAGTGTTTTCGCGACTCAGGCGATTGCGACGCCCAACTCTGTGCTCAGTGAGGAGGCCTTCAAGGGGCTTGGTGGGTTTTCAAAGGAGGAGCTAGACTCGGAGTCGGAGTATGAGTCGGAGAGTGAAGTGGCGGAGCCGGCGGTGGGGAGTAAAGACGAGCTGGCTCTTTCGAAATTGGGCTTGCCTCAGCGGCTTGTGGATAGCCTTGAGAGACGTGGCATTTCTTCGCTTTTTCCCATTCAGAGAGCTGTGCTAGTGCCTGCACTGGAAGGTAGAGACATTATAGCTCGTGCAAAGACCGGGACTGGGAAGACATTGGCCTTCGGGATTCCCATACTTAAACGCCTAACAGAAGATGATGAACAGAGAGGTTCAAGGCGGTCTGGTTATCTTCCTCGAGTTCTGGTCCTTGCACCTACTCGGGAGTTGGCAAAGCAAGTGGAGAAGGAGATTAAAGAGTCTGCACCTTATCTGAACACTGTTTGTGTCTATGGAGGAGTCTCCTACGTAACACAACAGAGTGCTCTTTCGCGAGGGGTTGATGTAGTGGTTGGAACTCCTGGTCGGCTTATTGACCTGATAAACGGTGGCAGCCTCAAGTTGAGTGAAGTTCAGTATTTGGTACTTGATGAAGCTGATCAGATGCTTGCTGTTGGATTTGAGGATGATGTGGAAACAATTTTACAAAGACTTCCAGCTCAGAGGCAGAGCATGCTTTTTTCTGCAACTATGCCAACTTGGGTGAAAAAGCTAGCACGGAAATATTTAGACAATCCATTGACGATTGATTTGGTTGGTGATCAAGACGAGAAACTTGCAGAAGGGATCAAACTCTACGCTCTATCAACCACTAACTCTTCAAAGCGGACTATTCTTAGTGATCTTATAACAGTTTATGCCAAGGGTGGGAAGACCATTGTTTTTACCCAAACCAAACGTGATGCTGATGAAGTGTCAATGTCATTAACGACTAGCATAGCTTCTGAGGCATTGCATGGAGATATATCACAACATCAAAGGGAGAGAACCCTAAATGGTTTTCGGCAAGGAAAATTCACAGTGCTCGTTGCCACTGATGTTGCATCCCGTGGACTTGATATTCCCAACGTTGACTTGATTATCCACTATGAACTTCCCAATGATTCAGAGACATTTGTACATCGCTCTGGTCGTACTGGACGTGCAGGGAAACTCGGAAATGCCGTTCTGATGTTTACTAATAACCAGAGGAGAACAGTCAAAACTCTTGAGCGTGATGTGGGGTGCAGGTTTGAGTTTGTTACTCCGCCAACAGTTGAAGAGGTTTTGGAGTCATCTGCTTCACATGTGGTTGCTACTCTCAATGGGGTTCATCCTGAGTCTATTAAGTTCTTCACACCAACAGCACAGAAACTGATTGAAGAACAGGGAACAACTGCTCTTGCTGCTGCACTGGCACAGCTAAGTGGATTTTCCCGACCTCCGTCATCCCGGTCTCTAATCAGTCACGAGCCGGGGTGGACAACATTGCAAATAATTCGGGATCCAGAGTTTGCCAGAGGTTACCTGTCTGCTAGATCTGTCACTGGGTTTCTTTCAGATGTTTATTCTGCAGCTGCTGATGAAGTTGGAAAAATATATTTAATTGCAGATGAAAGGGTTCAAGGAGCAGTTTTTGATCTTCCAGAGGAGATTGCAAAAGAGTTGTTGAAGAGGCAAATGCCTCCTGGAAACAGTATTTCCAAGATAAGCAAGTTGCCTCCACTGCAAGATGACGGTCCAGTGAGTGACAACTATGGCAGGTTTTCAGCTAGGGACAGGAGTAGTCGACGGGGAGGTTTTAGTGGTGGACGGGGAGGTGGTGGTGGAAGGGGAGGTTTTAGAAGCAGCTCTAGGGGTGGATGGGGAGGTGGCCGAGATTTTAGTGATGGTGATGATGATTTTAGGAGTAGCCGAGGAAGTTTTAATAGAAGTAGCAGCAGCAGCAGCCGACCACGGATGACAAGAAGTATGGATGATGATTGGCTTATAGGAGGTACACGAACAGGCAGAATACCATCCCGAGACAGAAGCTTTGGAGGTTCCTGTTTCAACTGTGGGCAGTCTGGGCACAGAGCATCAGAGTGCCCTTCTAAGAAACAAGGATATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000373 GO:0000375 GO:0000377 GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003729 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006970 GO:0006996 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009651 GO:0009657 GO:0009658 GO:0009941 GO:0009987 GO:0010467 GO:0010501 GO:0016020 GO:0016043 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022613 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0034641 GO:0042254 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0050896 GO:0070013 GO:0070035 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko01110 map01110

Pfam Domains

Protein Families

DEAD GUCT Helicase_C zf-CCHC

Protein Analysis

773

Amino Acids

83.73

Weight (kDa)

8.76

Isoelectric Point (pI)

47.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	142 - 313	1.3e-46	DEAD/DEAH box helicase
ResIII	PF04851	155 - 307	2e-06	Type III restriction enzyme, res subunit
Helicase_C	PF00271	351 - 459	2.7e-31	Helicase conserved C-terminal domain
DD_DDX21-DDX50	PF26142	494 - 543	6.5e-17	DDX21/DDX50 dimerisation domain
GUCT	PF08152	546 - 646	2.8e-16	GUCT (NUC152) domain
zf-CCHC	PF00098	751 - 767	1.3e-06	Zinc knuckle

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0008791)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G26742 AT5G26742 AT5G26742
fragaria_vesca	FvH4_6g08810 FvH4_6g08810 FvH4_6g08810 FvH4_6g08810
malus_domestica	MD04G1183400.v1.1 MD12G1196800.v1.1
prunus_persica	Prupe.6G301900_v2.0.a1
pyrus_communis	pycom04g16190 pycom12g18300
rosa_chinensis	RchiOBHm_Chr3g0459081
rosa_laevigata	RLG00000025121
rosa_multiflora	Rmu_sc0000466.1_g000002 Rmu_sc0003515.1_g000001
rosa_roxburghii	Rroxscaffold_6G00420530
rosa_rugosa	Rorug03G0033400
rosa_samantha	Rh3AG091300 Rh3BG094400 Rh3CG094800 Rh3DG094800
rosa_wichuraiana	Rw3G007840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1076
Acc36I	ACCTGC	2 cut(s)	1446, 1959
AccBSI	CCGCTC	2 cut(s)	71, 123
AccI	GTMKAC	1 cut(s)	1996
AccII	CGCG	2 cut(s)	200, 721
AccIII	TCCGGA	1 cut(s)	145
AclI	AACGTT	1 cut(s)	1296
AclWI	GGATC	3 cut(s)	1022, 1706, 1719
AcoI	YGGCCR	1 cut(s)	2080
AcsI	RAATTY	1 cut(s)	1241
AcyI	GRCGYC	1 cut(s)	218
AfaI	GTAC	5 cut(s)	56, 813, 1347, 1363, 2206
AfiI	CCNNNNNNNGG	4 cut(s)	252, 332, 495, 1095
AflIII	ACRYGT	1 cut(s)	1513
AhdI	GACNNNNNGTC	1 cut(s)	289
AjiI	CACGTC	2 cut(s)	404, 1370
AjnI	CCWGG	2 cut(s)	748, 1894
AloI	GAACNNNNNNTCC	2 cut(s)	1377, 1409
Alw21I	GWGCWC	3 cut(s)	234, 715, 1254
Alw26I	GTCTC	6 cut(s)	394, 459, 694, 1331, 1668, 2226
AlwI	GGATC	3 cut(s)	1022, 1706, 1719
AlwNI	CAGNNNCTG	2 cut(s)	1582, 1787
Ama87I	CYCGRG	3 cut(s)	585, 609, 2229
Aor13HI	TCCGGA	1 cut(s)	145
AoxI	GGCC	3 cut(s)	243, 507, 2080
ApoI	RAATTY	1 cut(s)	1241
ArsI	GACNNNNNNTTYG	4 cut(s)	961, 993, 2225, 2257
Asp700I	GAANNNNTTC	1 cut(s)	1827
AspS9I	GGNCC	2 cut(s)	595, 1946
AsuC2I	CCSGG	3 cut(s)	490, 1660, 1683
AsuHPI	GGTGA	3 cut(s)	943, 998, 2111
AsuII	TTCGAA	1 cut(s)	359
AsuNHI	GCTAGC	1 cut(s)	940
AvaI	CYCGRG	3 cut(s)	585, 609, 2229
AvaII	GGWCC	2 cut(s)	595, 1946
BaeGI	GKGCMC	3 cut(s)	165, 2280, 2297
BamHI	GGATCC	1 cut(s)	1711
BauI	CACGAG	2 cut(s)	477, 1676
BbsI	GAAGAC	2 cut(s)	506, 1106
Bbv12I	GWGCWC	3 cut(s)	234, 715, 1254
BbvCI	CCTCAGC	1 cut(s)	375
BccI	CCATC	3 cut(s)	2064, 2090, 2233
BceAI	ACGGC	3 cut(s)	108, 120, 1376
BcgI	CGANNNNNNTGC	4 cut(s)	986, 1020, 1688, 1722
BciT130I	CCWGG	2 cut(s)	750, 1896
BclI	TGATCA	2 cut(s)	826, 988
BcnI	CCSGG	3 cut(s)	490, 1660, 1683
BcoDI	GTCTC	6 cut(s)	394, 459, 694, 1331, 1668, 2226
BfaI	CTAG	8 cut(s)	77, 281, 443, 941, 1161, 1743, 1980, 2061
BfmI	CTRYAG	1 cut(s)	1779
BfuAI	ACCTGC	2 cut(s)	1446, 1959
BglI	GCCNNNNNGGC	2 cut(s)	92, 379
BglII	AGATCT	1 cut(s)	1745
Bme1390I	CCNGG	5 cut(s)	490, 750, 1660, 1683, 1896
Bme18I	GGWCC	2 cut(s)	595, 1946
BmeRI	GACNNNNNGTC	1 cut(s)	289
BmeT110I	CYCGRG	3 cut(s)	585, 609, 2229
BmgBI	CACGTC	2 cut(s)	404, 1370
BmgT120I	GGNCC	2 cut(s)	595, 1946
BmiI	GGNNCC	3 cut(s)	324, 1713, 2251
BmrFI	CCNGG	5 cut(s)	490, 750, 1660, 1683, 1896
BmrI	ACTGGG	2 cut(s)	504, 1764
BmsI	GCATC	4 cut(s)	822, 1120, 1280, 2294
BmtI	GCTAGC	1 cut(s)	944
BmuI	ACTGGG	2 cut(s)	504, 1764
BoxI	GACNNNNGTC	1 cut(s)	1651
BpiI	GAAGAC	2 cut(s)	506, 1106
BplI	GAGNNNNNCTC	2 cut(s)	269, 301
Bpu10I	CCTNAGC	1 cut(s)	375
Bpu14I	TTCGAA	1 cut(s)	359
BpuEI	CTTGAG	3 cut(s)	416, 770, 1457
BpuMI	CCSGG	3 cut(s)	490, 1660, 1683
BsaAI	YACGTR	1 cut(s)	697
BsaHI	GRCGYC	1 cut(s)	218
BsaI	GGTCTC	1 cut(s)	1668
BsaJI	CCNNGG	5 cut(s)	1089, 1276, 1682, 2122, 2162
BsaWI	WCCGGW	1 cut(s)	145
BsaXI	ACNNNNNCTCC	4 cut(s)	281, 287, 311, 317
Bsc4I	CCNNNNNNNGG	4 cut(s)	252, 332, 495, 1095
Bse118I	RCCGGY	1 cut(s)	325
Bse1I	ACTGG	6 cut(s)	459, 499, 1369, 1626, 1759, 1949
Bse3DI	GCAATG	2 cut(s)	1178, 1694
BseAI	TCCGGA	1 cut(s)	145
BseBI	CCWGG	2 cut(s)	750, 1896
BseDI	CCNNGG	5 cut(s)	1089, 1276, 1682, 2122, 2162
BseGI	GGATG	8 cut(s)	859, 1271, 1540, 1655, 2075, 2172, 2188, 2225
BseLI	CCNNNNNNNGG	4 cut(s)	252, 332, 495, 1095
BseMI	GCAATG	2 cut(s)	1178, 1694
BseMII	CTCAG	6 cut(s)	218, 247, 389, 902, 1164, 1536
BseNI	ACTGG	6 cut(s)	459, 499, 1369, 1626, 1759, 1949
BseRI	GAGGAG	7 cut(s)	63, 202, 254, 290, 699, 1432, 1871
BseSI	GKGCMC	3 cut(s)	165, 2280, 2297
BsgI	GTGCAG	5 cut(s)	435, 636, 1392, 1474, 1602
Bsh1236I	CGCG	2 cut(s)	200, 721
BshFI	GGCC	3 cut(s)	245, 509, 2082
BsiHKAI	GWGCWC	3 cut(s)	234, 715, 1254
BsiHKCI	CYCGRG	3 cut(s)	585, 609, 2229
BsiSI	CCGG	5 cut(s)	146, 326, 489, 1660, 1682
BslFI	GGGAC	2 cut(s)	505, 1997
BslI	CCNNNNNNNGG	4 cut(s)	252, 332, 495, 1095
BsmAI	GTCTC	6 cut(s)	394, 459, 694, 1331, 1668, 2226
BsmBI	CGTCTC	1 cut(s)	394
BsmFI	GGGAC	2 cut(s)	505, 1997
BsnI	GGCC	3 cut(s)	245, 509, 2082
Bso31I	GGTCTC	1 cut(s)	1668
BsoBI	CYCGRG	3 cut(s)	585, 609, 2229
Bsp119I	TTCGAA	1 cut(s)	359
Bsp1286I	GDGCHC	6 cut(s)	165, 234, 715, 1254, 2280, 2297
Bsp13I	TCCGGA	1 cut(s)	145
Bsp1407I	TGTACA	2 cut(s)	54, 1345
Bsp143I	GATC	7 cut(s)	826, 988, 1014, 1069, 1711, 1745, 1846
Bsp68I	TCGCGA	2 cut(s)	200, 721
BspANI	GGCC	3 cut(s)	245, 509, 2082
BspCNI	CTCAG	6 cut(s)	217, 246, 388, 901, 1165, 1537
BspEI	TCCGGA	1 cut(s)	145
BspFNI	CGCG	2 cut(s)	200, 721
BspLI	GGNNCC	3 cut(s)	324, 1713, 2251
BspMAI	CTGCAG	1 cut(s)	1783
BspMI	ACCTGC	2 cut(s)	1446, 1959
BspOI	GCTAGC	1 cut(s)	944
BspPI	GGATC	3 cut(s)	1022, 1706, 1719
BspT104I	TTCGAA	1 cut(s)	359
BspTNI	GGTCTC	1 cut(s)	1668
BsrBI	CCGCTC	2 cut(s)	71, 123
BsrDI	GCAATG	2 cut(s)	1178, 1694
BsrFI	RCCGGY	1 cut(s)	325
BsrGI	TGTACA	2 cut(s)	54, 1345
BsrI	ACTGG	6 cut(s)	459, 499, 1369, 1626, 1759, 1949
BssAI	RCCGGY	1 cut(s)	325
BssECI	CCNNGG	5 cut(s)	1089, 1276, 1682, 2122, 2162
BssMI	GATC	7 cut(s)	826, 988, 1014, 1069, 1711, 1745, 1846
BssNI	GRCGYC	1 cut(s)	218
BssSI	CACGAG	2 cut(s)	477, 1676
BssT1I	CCWWGG	1 cut(s)	1089
Bst2BI	CACGAG	2 cut(s)	477, 1676
Bst2UI	CCWGG	2 cut(s)	750, 1896
Bst6I	CTCTTC	3 cut(s)	1051, 1482, 1874
BstACI	GRCGYC	1 cut(s)	218
BstAPI	GCANNNNNTGC	1 cut(s)	901
BstAUI	TGTACA	2 cut(s)	54, 1345
BstBAI	YACGTR	1 cut(s)	697
BstBI	TTCGAA	1 cut(s)	359
BstC8I	GCNNGC	7 cut(s)	327, 351, 371, 450, 837, 902, 942
BstDEI	CTNAG	9 cut(s)	204, 233, 375, 888, 1064, 1173, 1545, 1631, 2301
BstDSI	CCRYGG	2 cut(s)	1276, 2162
BstEII	GGTNACC	1 cut(s)	1730
BstF5I	GGATG	8 cut(s)	859, 1271, 1540, 1655, 2075, 2172, 2188, 2225
BstFNI	CGCG	2 cut(s)	200, 721
BstKTI	GATC	7 cut(s)	829, 991, 1017, 1072, 1714, 1748, 1849
BstMAI	GTCTC	6 cut(s)	394, 459, 694, 1331, 1668, 2226
BstMBI	GATC	7 cut(s)	826, 988, 1014, 1069, 1711, 1745, 1846
BstNI	CCWGG	2 cut(s)	750, 1896
BstNSI	RCATGY	2 cut(s)	904, 1517
BstPAI	GACNNNNGTC	1 cut(s)	1651
BstPI	GGTNACC	1 cut(s)	1730
BstSCI	CCNGG	5 cut(s)	488, 748, 1658, 1681, 1894
BstSFI	CTRYAG	1 cut(s)	1779
BstSLI	GKGCMC	3 cut(s)	165, 2280, 2297
BstSNI	TACGTA	1 cut(s)	697
BstUI	CGCG	2 cut(s)	200, 721
BstV2I	GAAGAC	2 cut(s)	506, 1106
BstX2I	RGATCY	2 cut(s)	1711, 1745
BstYI	RGATCY	2 cut(s)	1711, 1745
BsuRI	GGCC	3 cut(s)	245, 509, 2082
BtgI	CCRYGG	2 cut(s)	1276, 2162
BtgZI	GCGATG	1 cut(s)	1334
BtrI	CACGTC	2 cut(s)	404, 1370
BtsCI	GGATG	8 cut(s)	859, 1271, 1540, 1655, 2075, 2172, 2188, 2225
BtsI	GCAGTG	1 cut(s)	1931
BtsIMutI	CAGTG	9 cut(s)	241, 452, 666, 1254, 1260, 1619, 1752, 1931, 1956
BtuMI	TCGCGA	2 cut(s)	200, 721
BveI	ACCTGC	2 cut(s)	1446, 1959
Cac8I	GCNNGC	7 cut(s)	327, 351, 371, 450, 837, 902, 942
CaiI	CAGNNNCTG	2 cut(s)	1582, 1787
Cfr10I	RCCGGY	1 cut(s)	325
Cfr13I	GGNCC	2 cut(s)	595, 1946
CseI	GACGC	1 cut(s)	226
Csp6I	GTAC	5 cut(s)	55, 812, 1346, 1362, 2205
CspCI	CAANNNNNGTGG	4 cut(s)	609, 644, 1249, 1284
CviAII	CATG	4 cut(s)	45, 901, 1184, 1514
CviQI	GTAC	5 cut(s)	55, 812, 1346, 1362, 2205
DdeI	CTNAG	9 cut(s)	204, 233, 375, 888, 1064, 1173, 1545, 1631, 2301
DpnI	GATC	7 cut(s)	828, 990, 1016, 1071, 1713, 1747, 1848
DpnII	GATC	7 cut(s)	826, 988, 1014, 1069, 1711, 1745, 1846
DriI	GACNNNNNGTC	1 cut(s)	289
EaeI	YGGCCR	1 cut(s)	2080
Eam1104I	CTCTTC	3 cut(s)	1051, 1482, 1874
Eam1105I	GACNNNNNGTC	1 cut(s)	289
EarI	CTCTTC	3 cut(s)	1051, 1482, 1874
EciI	GGCGGA	3 cut(s)	141, 335, 1464
Eco105I	TACGTA	1 cut(s)	697
Eco130I	CCWWGG	1 cut(s)	1089
Eco147I	AGGCCT	1 cut(s)	245
Eco31I	GGTCTC	1 cut(s)	1668
Eco47I	GGWCC	2 cut(s)	595, 1946
Eco88I	CYCGRG	3 cut(s)	585, 609, 2229
Eco91I	GGTNACC	1 cut(s)	1730
EcoO65I	GGTNACC	1 cut(s)	1730
EcoRII	CCWGG	2 cut(s)	748, 1894
EcoT14I	CCWWGG	1 cut(s)	1089
ErhI	CCWWGG	1 cut(s)	1089
Esp3I	CGTCTC	1 cut(s)	394
FaeI	CATG	4 cut(s)	48, 904, 1187, 1517
FaqI	GGGAC	2 cut(s)	505, 1997
FatI	CATG	4 cut(s)	44, 900, 1183, 1513
FbaI	TGATCA	2 cut(s)	826, 988
FblI	GTMKAC	1 cut(s)	1996
FokI	GGATG	8 cut(s)	866, 1258, 1527, 1642, 2082, 2179, 2195, 2212
FspBI	CTAG	8 cut(s)	77, 281, 443, 941, 1161, 1743, 1980, 2061
HaeIII	GGCC	3 cut(s)	245, 509, 2082
HapII	CCGG	5 cut(s)	146, 326, 489, 1660, 1682
HgaI	GACGC	1 cut(s)	226
Hin1I	GRCGYC	1 cut(s)	218
Hin1II	CATG	4 cut(s)	48, 904, 1187, 1517
HincII	GTYRAC	2 cut(s)	1300, 1997
HindII	GTYRAC	2 cut(s)	1300, 1997
HindIII	AAGCTT	1 cut(s)	2239
HpaII	CCGG	5 cut(s)	146, 326, 489, 1660, 1682
HphI	GGTGA	3 cut(s)	943, 998, 2111
Hpy166II	GTNNAC	7 cut(s)	1280, 1300, 1404, 1689, 1997, 2018, 2207
Hpy8I	GTNNAC	7 cut(s)	1280, 1300, 1404, 1689, 1997, 2018, 2207
Hpy99I	CGWCG	6 cut(s)	100, 103, 106, 112, 220, 2001
HpyAV	CCTTC	7 cut(s)	256, 452, 520, 628, 1004, 2031, 2307
HpyCH4IV	ACGT	5 cut(s)	403, 696, 1126, 1296, 1369
HpyF3I	CTNAG	9 cut(s)	204, 233, 375, 888, 1064, 1173, 1545, 1631, 2301
HpySE526I	ACGT	5 cut(s)	403, 696, 1126, 1296, 1369
Hsp92I	GRCGYC	1 cut(s)	218
Hsp92II	CATG	4 cut(s)	48, 904, 1187, 1517
Kpn2I	TCCGGA	1 cut(s)	145
KroI	GCCGGC	1 cut(s)	325
KroNI	GCCGGC	1 cut(s)	327
Ksp22I	TGATCA	2 cut(s)	826, 988
Kzo9I	GATC	7 cut(s)	826, 988, 1014, 1069, 1711, 1745, 1846
LmnI	GCTCC	5 cut(s)	76, 120, 277, 322, 1835
LweI	GCATC	4 cut(s)	822, 1120, 1280, 2294
MaeI	CTAG	8 cut(s)	77, 281, 443, 941, 1161, 1743, 1980, 2061
MaeII	ACGT	5 cut(s)	403, 696, 1126, 1296, 1369
MaeIII	GTNAC	6 cut(s)	697, 1468, 1673, 1730, 1750, 1955
MalI	GATC	7 cut(s)	828, 990, 1016, 1071, 1713, 1747, 1848
MbiI	CCGCTC	2 cut(s)	71, 123
MboI	GATC	7 cut(s)	826, 988, 1014, 1069, 1711, 1745, 1846
MflI	RGATCY	2 cut(s)	1711, 1745
MhlI	GDGCHC	6 cut(s)	165, 234, 715, 1254, 2280, 2297
MluCI	AATT	5 cut(s)	362, 867, 1241, 1704, 1812
MlyI	GAGTC	9 cut(s)	34, 196, 278, 299, 311, 656, 696, 1508, 1556
MmeI	TCCRAC	5 cut(s)	273, 285, 721, 823, 1777
MroI	TCCGGA	1 cut(s)	145
MroNI	GCCGGC	1 cut(s)	325
MroXI	GAANNNNTTC	1 cut(s)	1827
MseI	TTAA	6 cut(s)	528, 642, 1154, 1554, 1811, 2133
MslI	CAYNNNNRTG	1 cut(s)	2290
MspA1I	CMGCKG	2 cut(s)	379, 1784
MspI	CCGG	5 cut(s)	146, 326, 489, 1660, 1682
MspR9I	CCNGG	5 cut(s)	490, 750, 1660, 1683, 1896
MvaI	CCWGG	2 cut(s)	750, 1896
MvnI	CGCG	2 cut(s)	200, 721
NaeI	GCCGGC	1 cut(s)	327
NciI	CCSGG	3 cut(s)	490, 1660, 1683
NdeII	GATC	7 cut(s)	826, 988, 1014, 1069, 1711, 1745, 1846
NgoMIV	GCCGGC	1 cut(s)	325
NheI	GCTAGC	1 cut(s)	940
NlaIII	CATG	4 cut(s)	48, 904, 1187, 1517
NlaIV	GGNNCC	3 cut(s)	324, 1713, 2251
NmeAIII	GCCGAG	2 cut(s)	2108, 2147
NmuCI	GTSAC	3 cut(s)	1673, 1750, 1955
NruI	TCGCGA	2 cut(s)	200, 721
NspI	RCATGY	2 cut(s)	904, 1517
NspV	TTCGAA	1 cut(s)	359
PaeI	GCATGC	1 cut(s)	904
PaeR7I	CTCGAG	1 cut(s)	585
PceI	AGGCCT	1 cut(s)	245
PciI	ACATGT	1 cut(s)	1513
PcsI	WCGNNNNNNNCGW	6 cut(s)	98, 104, 107, 1357, 1366, 1389
PdiI	GCCGGC	1 cut(s)	327
PdmI	GAANNNNTTC	1 cut(s)	1827
PfeI	GAWTC	2 cut(s)	517, 1331
PfoI	TCCNGGA	1 cut(s)	1894
PleI	GAGTC	9 cut(s)	34, 196, 278, 298, 310, 655, 695, 1507, 1555
PpsI	GAGTC	9 cut(s)	34, 196, 278, 298, 310, 655, 695, 1507, 1555
Ppu21I	YACGTR	1 cut(s)	697
PscI	ACATGT	1 cut(s)	1513
PshAI	GACNNNNGTC	1 cut(s)	1651
PsiI	TTATAA	1 cut(s)	1076
Psp1406I	AACGTT	1 cut(s)	1296
Psp6I	CCWGG	2 cut(s)	748, 1894
PspEI	GGTNACC	1 cut(s)	1730
PspGI	CCWGG	2 cut(s)	748, 1894
PspN4I	GGNNCC	3 cut(s)	324, 1713, 2251
PspPI	GGNCC	2 cut(s)	595, 1946
PspXI	VCTCGAGB	1 cut(s)	585
PstI	CTGCAG	1 cut(s)	1783
PstNI	CAGNNNCTG	2 cut(s)	1582, 1787
PsuI	RGATCY	2 cut(s)	1711, 1745
PvuII	CAGCTG	1 cut(s)	1784
RruI	TCGCGA	2 cut(s)	200, 721
RsaI	GTAC	5 cut(s)	56, 813, 1347, 1363, 2206
RsaNI	GTAC	5 cut(s)	55, 812, 1346, 1362, 2205
RseI	CAYNNNNRTG	1 cut(s)	2290
SalI	GTCGAC	1 cut(s)	1995
SaqAI	TTAA	6 cut(s)	528, 642, 1154, 1554, 1811, 2133
Sau3AI	GATC	7 cut(s)	826, 988, 1014, 1069, 1711, 1745, 1846
Sau96I	GGNCC	2 cut(s)	595, 1946
SchI	GAGTC	9 cut(s)	34, 196, 278, 299, 311, 656, 696, 1508, 1556
ScrFI	CCNGG	5 cut(s)	490, 750, 1660, 1683, 1896
SduI	GDGCHC	6 cut(s)	165, 234, 715, 1254, 2280, 2297
SfaNI	GCATC	4 cut(s)	822, 1120, 1280, 2294
SfcI	CTRYAG	1 cut(s)	1779
Sfr274I	CTCGAG	1 cut(s)	585
SfuI	TTCGAA	1 cut(s)	359
SinI	GGWCC	2 cut(s)	595, 1946
SlaI	CTCGAG	1 cut(s)	585
SmiMI	CAYNNNNRTG	1 cut(s)	2290
SmlI	CTYRAG	4 cut(s)	395, 585, 785, 1436
SmoI	CTYRAG	4 cut(s)	395, 585, 785, 1436
SnaBI	TACGTA	1 cut(s)	697
SphI	GCATGC	1 cut(s)	904
Sse9I	AATT	5 cut(s)	362, 867, 1241, 1704, 1812
SseBI	AGGCCT	1 cut(s)	245
SspI	AATATT	1 cut(s)	953
SspMI	CTAG	8 cut(s)	77, 281, 443, 941, 1161, 1743, 1980, 2061
StuI	AGGCCT	1 cut(s)	245
StyD4I	CCNGG	5 cut(s)	488, 748, 1658, 1681, 1894
StyI	CCWWGG	1 cut(s)	1089
TaiI	ACGT	5 cut(s)	406, 699, 1129, 1299, 1372
TaqI	TCGA	3 cut(s)	359, 586, 1996
TasI	AATT	5 cut(s)	362, 867, 1241, 1704, 1812
TatI	WGTACW	2 cut(s)	54, 1345
TauI	GCSGC	4 cut(s)	71, 86, 126, 382
TfiI	GAWTC	2 cut(s)	517, 1331
Tru1I	TTAA	6 cut(s)	528, 642, 1154, 1554, 1811, 2133
Tru9I	TTAA	6 cut(s)	528, 642, 1154, 1554, 1811, 2133
TscAI	CASTG	9 cut(s)	241, 459, 673, 1254, 1267, 1626, 1759, 1938, 1956
TseFI	GTSAC	3 cut(s)	1673, 1750, 1955
Tsp45I	GTSAC	3 cut(s)	1673, 1750, 1955
TspDTI	ATGAA	7 cut(s)	564, 834, 1152, 1332, 1529, 1806, 1836
TspGWI	ACGGA	3 cut(s)	961, 1641, 2179
TspRI	CASTG	9 cut(s)	241, 459, 673, 1254, 1267, 1626, 1759, 1938, 1956
VpaK11BI	GGWCC	2 cut(s)	595, 1946
XapI	RAATTY	1 cut(s)	1241
XceI	RCATGY	2 cut(s)	904, 1517
XhoI	CTCGAG	1 cut(s)	585
XmiI	GTMKAC	1 cut(s)	1996
XmnI	GAANNNNTTC	1 cut(s)	1827
XspI	CTAG	8 cut(s)	77, 281, 443, 941, 1161, 1743, 1980, 2061

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.