Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0008791

Physical Location & Seq

Genomic Coordinates Forward (+)

7601825 .. 7607416

Gene Structure 5592 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ42569

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2280 bp

ATGGCTTCGATTATCGGAGTTTCCTCTATATACTACCAGACTCCATGTGAGTTGTATAAAAGAGCTGCCGCGCCTGTAGCTTCCACGACGGCCCCGGCGACGAGCAGTGGCGGCGGCGGCGGTGTTTCGTTGGCGTTTCCGGAGAGGCAGAGGGCGCACTTCAACAGTGTTTTGAGAGCGAGGAGTGTTTTCGCTACTCAGGCGATTGCGACGCCTAATTCTGTGCTCAGTGAAGAGGCCTTCAAGGGGCTAGGGGACTTTTCCAAGGGGGAGCTGGACTCGGAGTCGGAGTATGAGTCGGAGAGTGATGTGGTTGAGCCGGTTAGTAAAGACGAGCTGGCTCTTTCGAAATTGGGCCTGCCTCAGAGGCTTGCGGATAGTCTTGAGAGGCGTGGCATTTCTTCGCTTTTTCCCATTCAGAGAGCTGTGCTAGTGCCGGCACTGGAAGGTCGAGACATCATAGCTCGTGCAAAGACTGGGACTGGGAAGACATTGGCCTTTGGGATTCCCATACTTAAACGCCTTACAGAAGATGATGAACAGAGAGGTTCAAGGCGGTCTGGTTATCTTCCTAGAGTTCTGGTCCTTGCACCAACTCGGGAGTTAGCGAAGCAAGTGGACAAGGAGATTAAAGAATCTGCACCTTATCTGAACACTGTTTGCGTCTATGGAGGAGTTTCATACGTAACGCAACAGAGTGCTCTTTCTCGTGGGGTTGACGTAGTGGTTGGAACTCCTGGTCGGCTTATTGACCTGATAAACGGCGGCAGCCTCAAGTTGGGTGAAGTTCAATATTTGGTACTTGATGAAGCTGATCAGATGCTTGCTGTTGGATTTGAGGAAGATGTGGAAACAATTCTACAAAGGCTCCCAGCTCAGAGGCAGAGCATGCTTTTTTCTGCAACTATGCCCACTTGGGTGAAAAAACTAGCACGAAAATATTTGGACAATCCATTGACGATTGATTTGGTTGGTGATCAAGATGAGAAGCTTGCAGAAGGGATCAAACTCTATGCTCTATCAACCACAAACTCTTCAAAGCGGACTATTCTTAGTGATCTTATAACAGTTTATGCAAAGGGTGGGAAGACCATTGTTTTTACACAAACAAAACGTGATGCTGATGAAGTGTCAATGTCATTGACGACTAGCATAGCTTCTGAGGCATTGCATGGAGATATATCACAACATCAAAGGGAGAGAACCCTTAATGGTTTTCGGCAAGGAAAATTCACTGTGCTCGTTGCCACTGATGTTGCATCCCGTGGACTTGATATTCCCAACGTTGACTTGGTTATCCACTATGAACTTCCCAATGATTCAGAGACATTTGTACATCGCTCTGGTCGTACTGGACGTGCAGGGAAACTCGGTAATGCCGTTCTGATGTTTACTAATAACCAGAGGAGAACAGTCAAAACTCTTGAGCGTGATGTGGGATGCAGGTTTGAGTTTGTTACTCCGCCAACCGTTGAGGAGGTTTTGGAGTCATCTGCTTCACATGTGGTTGCTACTCTCAATGGGGTTCATCCTGAATCCATACAGTTTTTTACACCAACGGCACAGAGACTGATTGAAGAACAGGGAACAACTGCTCTTGCTGCTGCACTGGCACAGCTAAGTGGATTTTCCCGACCTCCGTCATCCCGGTCTCTAATCAGTCATGAGCCGGGGTGGACGACATTGCAAATAATTCGGGATCCAGCTTTTGCCAGAGGTTACCTGTCTGCTAGATCTGTCACTGGGTTTCTTTCAGATGTTTATTCTGCAGCTGCTGATGAACTTGGAAAAATATATTTAATTGCAGATGAAAGGGTTTTTCCAGAGGAGATTGCAAAAGAGTTGCTGAAGAGGCAAATACCTCCTGGAAACAGTATTTCCAAGATCAGCAAGTTGCCTTCTCTGCAAGATGACGGGCCAGTGAGTGACAACTATGGCAGGTTTTCAGCTAGGGATCGGAGTAGTCGACGGGGAAGTTTTAGTAGTGGACGGGGAGGTAGTGGTGGACGGGGAGGTTTTAGAAGTAGCTCTAGGGGTGGATGGGGAGGCGGCCGAGGTTCTAGTGATGGTGATGATGATTTTAGGAGTAGCAGAGGAAGTTTTAATAGATCTAGCAGCAGCCGACCTCGGATGACAAGAAGTATGGATGATGATTGGCTTATTGGAGGTACACGAACAGGCAGAATACCACCACGAGACAGAAGCTTTGGAGGTTCCTGTTTCAATTGTGGGCAGTCTGGGCACAGGGCATCAGAGTGTCCTCAGAAACAAGGATATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000373 GO:0000375 GO:0000377 GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003729 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006970 GO:0006996 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009651 GO:0009657 GO:0009658 GO:0009941 GO:0009987 GO:0010467 GO:0010501 GO:0016020 GO:0016043 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022613 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0034641 GO:0042254 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0050896 GO:0070013 GO:0070035 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko01110 map01110

Pfam Domains

Protein Families

DEAD GUCT Helicase_C zf-CCHC

Protein Analysis

759

Amino Acids

82.24

Weight (kDa)

8.78

Isoelectric Point (pI)

45.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	138 - 309	1.6e-45	DEAD/DEAH box helicase
ResIII	PF04851	151 - 303	3.5e-06	Type III restriction enzyme, res subunit
Helicase_C	PF00271	347 - 455	8e-32	Helicase conserved C-terminal domain
DD_DDX21-DDX50	PF26142	490 - 539	1e-16	DDX21/DDX50 dimerisation domain
GUCT	PF08152	542 - 636	3.7e-07	GUCT (NUC152) domain
zf-CCHC	PF00098	739 - 755	1.1e-06	Zinc knuckle

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0008791)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G26742 AT5G26742 AT5G26742
fragaria_vesca	FvH4_6g08810 FvH4_6g08810 FvH4_6g08810 FvH4_6g08810
malus_domestica	MD04G1183400.v1.1 MD12G1196800.v1.1
prunus_persica	Prupe.6G301900_v2.0.a1
pyrus_communis	pycom04g16190 pycom12g18300
rosa_chinensis	RchiOBHm_Chr3g0459081
rosa_laevigata	RLG00000025121
rosa_multiflora	Rmu_sc0000466.1_g000002 Rmu_sc0003515.1_g000001
rosa_roxburghii	Rroxscaffold_6G00420530
rosa_rugosa	Rorug03G0033400
rosa_samantha	Rh3AG091300 Rh3BG094400 Rh3CG094800 Rh3DG094800
rosa_wichuraiana	Rw3G007840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1064
Acc36I	ACCTGC	2 cut(s)	1434, 1929
AccI	GTMKAC	1 cut(s)	1966
AccII	CGCG	1 cut(s)	71
AccIII	TCCGGA	1 cut(s)	139
AclI	AACGTT	1 cut(s)	1284
AclWI	GGATC	4 cut(s)	1010, 1694, 1707, 1962
AcoI	YGGCCR	1 cut(s)	2050
AcsI	RAATTY	1 cut(s)	1229
AcuI	CTGAAG	1 cut(s)	1868
AcyI	GRCGYC	1 cut(s)	212
AfaI	GTAC	4 cut(s)	801, 1335, 1351, 2170
AfiI	CCNNNNNNNGG	2 cut(s)	246, 778
AflIII	ACRYGT	1 cut(s)	1501
AgsI	TTSAA	7 cut(s)	163, 244, 552, 791, 1038, 1577, 2224
AhdI	GACNNNNNGTC	1 cut(s)	283
AjiI	CACGTC	1 cut(s)	1358
AjnI	CCWGG	2 cut(s)	736, 1864
AleI	CACNNNNGTG	1 cut(s)	917
Alw21I	GWGCWC	3 cut(s)	228, 703, 1242
Alw26I	GTCTC	5 cut(s)	447, 1319, 1561, 1656, 2190
AlwI	GGATC	4 cut(s)	1010, 1694, 1707, 1962
AlwNI	CAGNNNCTG	2 cut(s)	1570, 1775
Ama87I	CYCGRG	1 cut(s)	597
Aor13HI	TCCGGA	1 cut(s)	139
AoxI	GGCC	6 cut(s)	90, 237, 355, 495, 1916, 2050
ApeKI	GCWGC	8 cut(s)	65, 768, 1601, 1604, 1769, 1772, 2115, 2118
ApoI	RAATTY	1 cut(s)	1229
ArsI	GACNNNNNNTTYG	4 cut(s)	949, 981, 2189, 2221
Asp700I	GAANNNNTTC	1 cut(s)	855
AspLEI	GCGC	2 cut(s)	73, 157
AspS9I	GGNCC	4 cut(s)	91, 355, 583, 1916
AsuC2I	CCSGG	3 cut(s)	95, 1648, 1671
AsuHPI	GGTGA	4 cut(s)	794, 931, 986, 2081
AsuII	TTCGAA	1 cut(s)	347
AvaI	CYCGRG	1 cut(s)	597
AvaII	GGWCC	1 cut(s)	583
BaeGI	GKGCMC	1 cut(s)	2244
BamHI	GGATCC	1 cut(s)	1699
BauI	CACGAG	3 cut(s)	465, 708, 2193
BbsI	GAAGAC	2 cut(s)	494, 1094
Bbv12I	GWGCWC	3 cut(s)	228, 703, 1242
BbvI	GCAGC	8 cut(s)	52, 780, 1588, 1591, 1759, 1781, 2127, 2130
BccI	CCATC	2 cut(s)	2034, 2060
BceAI	ACGGC	4 cut(s)	105, 778, 1364, 1575
BcgI	CGANNNNNNTGC	2 cut(s)	1676, 1710
BciT130I	CCWGG	2 cut(s)	738, 1866
BclI	TGATCA	2 cut(s)	814, 976
BcnI	CCSGG	3 cut(s)	95, 1648, 1671
BcoDI	GTCTC	5 cut(s)	447, 1319, 1561, 1656, 2190
BfmI	CTRYAG	2 cut(s)	75, 1767
BfuAI	ACCTGC	2 cut(s)	1434, 1929
BglI	GCCNNNNNGGC	1 cut(s)	367
BglII	AGATCT	2 cut(s)	1733, 2108
Bme1390I	CCNGG	5 cut(s)	95, 738, 1648, 1671, 1866
Bme18I	GGWCC	1 cut(s)	583
BmeRI	GACNNNNNGTC	1 cut(s)	283
BmeT110I	CYCGRG	1 cut(s)	597
BmgBI	CACGTC	1 cut(s)	1358
BmgT120I	GGNCC	4 cut(s)	91, 355, 583, 1916
BmiI	GGNNCC	4 cut(s)	93, 869, 1701, 2215
BmrFI	CCNGG	5 cut(s)	95, 738, 1648, 1671, 1866
BmrI	ACTGGG	3 cut(s)	486, 492, 1752
BmsI	GCATC	5 cut(s)	810, 1108, 1268, 1430, 2258
BmuI	ACTGGG	3 cut(s)	486, 492, 1752
BoxI	GACNNNNGTC	1 cut(s)	1639
BpiI	GAAGAC	2 cut(s)	494, 1094
BplI	GAGNNNNNCTC	2 cut(s)	263, 295
Bpu14I	TTCGAA	1 cut(s)	347
BpuEI	CTTGAG	3 cut(s)	404, 758, 1445
BpuMI	CCSGG	3 cut(s)	95, 1648, 1671
BsaAI	YACGTR	1 cut(s)	685
BsaHI	GRCGYC	1 cut(s)	212
BsaI	GGTCTC	1 cut(s)	1656
BsaJI	CCNNGG	6 cut(s)	93, 264, 1264, 1670, 2053, 2126
BsaWI	WCCGGW	1 cut(s)	139
BsaXI	ACNNNNNCTCC	6 cut(s)	275, 281, 305, 311, 852, 882
Bsc4I	CCNNNNNNNGG	2 cut(s)	246, 778
Bse118I	RCCGGY	2 cut(s)	319, 436
Bse1I	ACTGG	7 cut(s)	447, 481, 487, 1357, 1614, 1747, 1919
Bse3DI	GCAATG	2 cut(s)	1166, 1682
BseAI	TCCGGA	1 cut(s)	139
BseBI	CCWGG	2 cut(s)	738, 1866
BseDI	CCNNGG	6 cut(s)	93, 264, 1264, 1670, 2053, 2126
BseGI	GGATG	7 cut(s)	1259, 1445, 1528, 1643, 2045, 2136, 2152
BseLI	CCNNNNNNNGG	2 cut(s)	246, 778
BseMI	GCAATG	2 cut(s)	1166, 1682
BseMII	CTCAG	6 cut(s)	212, 241, 377, 890, 1152, 2276
BseNI	ACTGG	7 cut(s)	447, 481, 487, 1357, 1614, 1747, 1919
BseRI	GAGGAG	5 cut(s)	196, 687, 1420, 1490, 1841
BseSI	GKGCMC	1 cut(s)	2244
BseX3I	CGGCCG	1 cut(s)	2050
BseXI	GCAGC	8 cut(s)	52, 780, 1588, 1591, 1759, 1781, 2127, 2130
BseYI	CCCAGC	1 cut(s)	871
BsgI	GTGCAG	3 cut(s)	624, 1380, 1590
Bsh1236I	CGCG	1 cut(s)	71
Bsh1285I	CGRYCG	1 cut(s)	2053
BshFI	GGCC	6 cut(s)	92, 239, 357, 497, 1918, 2052
BsiEI	CGRYCG	1 cut(s)	2053
BsiHKAI	GWGCWC	3 cut(s)	228, 703, 1242
BsiHKCI	CYCGRG	1 cut(s)	597
BsiSI	CCGG	6 cut(s)	95, 140, 320, 437, 1648, 1670
BslFI	GGGAC	2 cut(s)	269, 493
BslI	CCNNNNNNNGG	2 cut(s)	246, 778
BsmAI	GTCTC	5 cut(s)	447, 1319, 1561, 1656, 2190
BsmFI	GGGAC	2 cut(s)	269, 493
BsnI	GGCC	6 cut(s)	92, 239, 357, 497, 1918, 2052
Bso31I	GGTCTC	1 cut(s)	1656
BsoBI	CYCGRG	1 cut(s)	597
Bsp119I	TTCGAA	1 cut(s)	347
Bsp1286I	GDGCHC	4 cut(s)	228, 703, 1242, 2244
Bsp13I	TCCGGA	1 cut(s)	139
Bsp1407I	TGTACA	1 cut(s)	1333
Bsp143I	GATC	9 cut(s)	814, 976, 1002, 1057, 1699, 1733, 1884, 1954, 2108
BspANI	GGCC	6 cut(s)	92, 239, 357, 497, 1918, 2052
BspCNI	CTCAG	6 cut(s)	211, 240, 376, 889, 1153, 2275
BspEI	TCCGGA	1 cut(s)	139
BspFNI	CGCG	1 cut(s)	71
BspHI	TCATGA	1 cut(s)	1663
BspLI	GGNNCC	4 cut(s)	93, 869, 1701, 2215
BspMAI	CTGCAG	1 cut(s)	1771
BspMI	ACCTGC	2 cut(s)	1434, 1929
BspPI	GGATC	4 cut(s)	1010, 1694, 1707, 1962
BspT104I	TTCGAA	1 cut(s)	347
BspTNI	GGTCTC	1 cut(s)	1656
BsrDI	GCAATG	2 cut(s)	1166, 1682
BsrFI	RCCGGY	2 cut(s)	319, 436
BsrGI	TGTACA	1 cut(s)	1333
BsrI	ACTGG	7 cut(s)	447, 481, 487, 1357, 1614, 1747, 1919
BssAI	RCCGGY	2 cut(s)	319, 436
BssECI	CCNNGG	6 cut(s)	93, 264, 1264, 1670, 2053, 2126
BssMI	GATC	9 cut(s)	814, 976, 1002, 1057, 1699, 1733, 1884, 1954, 2108
BssNI	GRCGYC	1 cut(s)	212
BssSI	CACGAG	3 cut(s)	465, 708, 2193
BssT1I	CCWWGG	1 cut(s)	264
Bst2BI	CACGAG	3 cut(s)	465, 708, 2193
Bst2UI	CCWGG	2 cut(s)	738, 1866
Bst4CI	ACNGT	8 cut(s)	167, 658, 1069, 1237, 1414, 1471, 1545, 1874
Bst6I	CTCTTC	3 cut(s)	228, 1039, 1844
BstACI	GRCGYC	1 cut(s)	212
BstAPI	GCANNNNNTGC	1 cut(s)	889
BstAUI	TGTACA	1 cut(s)	1333
BstBAI	YACGTR	1 cut(s)	685
BstBI	TTCGAA	1 cut(s)	347
BstC8I	GCNNGC	7 cut(s)	339, 359, 372, 438, 825, 890, 993
BstDEI	CTNAG	8 cut(s)	198, 227, 363, 876, 1052, 1161, 1619, 2262
BstDSI	CCRYGG	1 cut(s)	1264
BstEII	GGTNACC	1 cut(s)	1718
BstF5I	GGATG	7 cut(s)	1259, 1445, 1528, 1643, 2045, 2136, 2152
BstFNI	CGCG	1 cut(s)	71
BstHHI	GCGC	2 cut(s)	73, 157
BstKTI	GATC	9 cut(s)	817, 979, 1005, 1060, 1702, 1736, 1887, 1957, 2111
BstMAI	GTCTC	5 cut(s)	447, 1319, 1561, 1656, 2190
BstMBI	GATC	9 cut(s)	814, 976, 1002, 1057, 1699, 1733, 1884, 1954, 2108
BstMCI	CGRYCG	1 cut(s)	2053
BstNI	CCWGG	2 cut(s)	738, 1866
BstNSI	RCATGY	2 cut(s)	892, 1505
BstPAI	GACNNNNGTC	1 cut(s)	1639
BstPI	GGTNACC	1 cut(s)	1718
BstSCI	CCNGG	5 cut(s)	93, 736, 1646, 1669, 1864
BstSFI	CTRYAG	2 cut(s)	75, 1767
BstSLI	GKGCMC	1 cut(s)	2244
BstSNI	TACGTA	1 cut(s)	685
BstUI	CGCG	1 cut(s)	71
BstV1I	GCAGC	8 cut(s)	52, 780, 1588, 1591, 1759, 1781, 2127, 2130
BstV2I	GAAGAC	2 cut(s)	494, 1094
BstX2I	RGATCY	3 cut(s)	1699, 1733, 2108
BstYI	RGATCY	3 cut(s)	1699, 1733, 2108
BstZI	CGGCCG	1 cut(s)	2050
BsuRI	GGCC	6 cut(s)	92, 239, 357, 497, 1918, 2052
BtgI	CCRYGG	1 cut(s)	1264
BtgZI	GCGATG	1 cut(s)	1322
BtrI	CACGTC	1 cut(s)	1358
BtsCI	GGATG	7 cut(s)	1259, 1445, 1528, 1643, 2045, 2136, 2152
BtsI	GCAGTG	1 cut(s)	112
BveI	ACCTGC	2 cut(s)	1434, 1929
Cac8I	GCNNGC	7 cut(s)	339, 359, 372, 438, 825, 890, 993
CaiI	CAGNNNCTG	2 cut(s)	1570, 1775
CciI	TCATGA	1 cut(s)	1663
CfoI	GCGC	2 cut(s)	73, 157
Cfr10I	RCCGGY	2 cut(s)	319, 436
Cfr13I	GGNCC	4 cut(s)	91, 355, 583, 1916
CseI	GACGC	2 cut(s)	220, 652
Csp6I	GTAC	4 cut(s)	800, 1334, 1350, 2169
CspCI	CAANNNNNGTGG	2 cut(s)	1237, 1272
CviAII	CATG	5 cut(s)	45, 889, 1172, 1502, 1664
CviQI	GTAC	4 cut(s)	800, 1334, 1350, 2169
DdeI	CTNAG	8 cut(s)	198, 227, 363, 876, 1052, 1161, 1619, 2262
DpnI	GATC	9 cut(s)	816, 978, 1004, 1059, 1701, 1735, 1886, 1956, 2110
DpnII	GATC	9 cut(s)	814, 976, 1002, 1057, 1699, 1733, 1884, 1954, 2108
DriI	GACNNNNNGTC	1 cut(s)	283
EaeI	YGGCCR	1 cut(s)	2050
EagI	CGGCCG	1 cut(s)	2050
Eam1104I	CTCTTC	3 cut(s)	228, 1039, 1844
Eam1105I	GACNNNNNGTC	1 cut(s)	283
EarI	CTCTTC	3 cut(s)	228, 1039, 1844
EciI	GGCGGA	1 cut(s)	1452
EclXI	CGGCCG	1 cut(s)	2050
Eco105I	TACGTA	1 cut(s)	685
Eco130I	CCWWGG	1 cut(s)	264
Eco147I	AGGCCT	1 cut(s)	239
Eco31I	GGTCTC	1 cut(s)	1656
Eco47I	GGWCC	1 cut(s)	583
Eco52I	CGGCCG	1 cut(s)	2050
Eco57I	CTGAAG	1 cut(s)	1868
Eco88I	CYCGRG	1 cut(s)	597
Eco91I	GGTNACC	1 cut(s)	1718
EcoO65I	GGTNACC	1 cut(s)	1718
EcoRII	CCWGG	2 cut(s)	736, 1864
EcoT14I	CCWWGG	1 cut(s)	264
ErhI	CCWWGG	1 cut(s)	264
FaeI	CATG	5 cut(s)	48, 892, 1175, 1505, 1667
FaqI	GGGAC	2 cut(s)	269, 493
FatI	CATG	5 cut(s)	44, 888, 1171, 1501, 1663
FbaI	TGATCA	2 cut(s)	814, 976
FblI	GTMKAC	1 cut(s)	1966
FokI	GGATG	7 cut(s)	1246, 1452, 1515, 1630, 2052, 2143, 2159
GlaI	GCGC	2 cut(s)	72, 156
GsaI	CCCAGC	1 cut(s)	875
HaeIII	GGCC	6 cut(s)	92, 239, 357, 497, 1918, 2052
HapII	CCGG	6 cut(s)	95, 140, 320, 437, 1648, 1670
HgaI	GACGC	2 cut(s)	220, 652
HhaI	GCGC	2 cut(s)	73, 157
Hin1I	GRCGYC	1 cut(s)	212
Hin1II	CATG	5 cut(s)	48, 892, 1175, 1505, 1667
Hin6I	GCGC	2 cut(s)	71, 155
HinP1I	GCGC	2 cut(s)	71, 155
HincII	GTYRAC	3 cut(s)	718, 1288, 1967
HindII	GTYRAC	3 cut(s)	718, 1288, 1967
HindIII	AAGCTT	2 cut(s)	989, 2203
HinfI	GANTC	9 cut(s)	40, 278, 284, 296, 505, 635, 1319, 1487, 1535
HpaII	CCGG	6 cut(s)	95, 140, 320, 437, 1648, 1670
HphI	GGTGA	4 cut(s)	794, 931, 986, 2081
Hpy99I	CGWCG	4 cut(s)	91, 103, 214, 1971
HpyAV	CCTTC	4 cut(s)	250, 440, 992, 1908
HpyCH4III	ACNGT	8 cut(s)	167, 658, 1069, 1237, 1414, 1471, 1545, 1874
HpyCH4IV	ACGT	5 cut(s)	684, 720, 1114, 1284, 1357
HpyF3I	CTNAG	8 cut(s)	198, 227, 363, 876, 1052, 1161, 1619, 2262
HpySE526I	ACGT	5 cut(s)	684, 720, 1114, 1284, 1357
Hsp92I	GRCGYC	1 cut(s)	212
Hsp92II	CATG	5 cut(s)	48, 892, 1175, 1505, 1667
HspAI	GCGC	2 cut(s)	71, 155
Kpn2I	TCCGGA	1 cut(s)	139
KroI	GCCGGC	1 cut(s)	436
KroNI	GCCGGC	1 cut(s)	438
Ksp22I	TGATCA	2 cut(s)	814, 976
Kzo9I	GATC	9 cut(s)	814, 976, 1002, 1057, 1699, 1733, 1884, 1954, 2108
LmnI	GCTCC	2 cut(s)	271, 873
Lsp1109I	GCAGC	8 cut(s)	52, 780, 1588, 1591, 1759, 1781, 2127, 2130
LweI	GCATC	5 cut(s)	810, 1108, 1268, 1430, 2258
MaeII	ACGT	5 cut(s)	684, 720, 1114, 1284, 1357
MaeIII	GTNAC	5 cut(s)	685, 1456, 1718, 1738, 1925
MalI	GATC	9 cut(s)	816, 978, 1004, 1059, 1701, 1735, 1886, 1956, 2110
MboI	GATC	9 cut(s)	814, 976, 1002, 1057, 1699, 1733, 1884, 1954, 2108
MfeI	CAATTG	1 cut(s)	2224
MflI	RGATCY	3 cut(s)	1699, 1733, 2108
MhlI	GDGCHC	4 cut(s)	228, 703, 1242, 2244
MluCI	AATT	7 cut(s)	217, 350, 855, 1229, 1692, 1800, 2224
MlyI	GAGTC	5 cut(s)	34, 272, 293, 305, 1496
MmeI	TCCRAC	4 cut(s)	267, 279, 709, 811
MroI	TCCGGA	1 cut(s)	139
MroNI	GCCGGC	1 cut(s)	436
MroXI	GAANNNNTTC	1 cut(s)	855
MseI	TTAA	5 cut(s)	516, 630, 1209, 1799, 2103
MslI	CAYNNNNRTG	2 cut(s)	917, 2254
MspA1I	CMGCKG	1 cut(s)	1772
MspI	CCGG	6 cut(s)	95, 140, 320, 437, 1648, 1670
MspR9I	CCNGG	5 cut(s)	95, 738, 1648, 1671, 1866
MunI	CAATTG	1 cut(s)	2224
MvaI	CCWGG	2 cut(s)	738, 1866
MvnI	CGCG	1 cut(s)	71
NaeI	GCCGGC	1 cut(s)	438
NciI	CCSGG	3 cut(s)	95, 1648, 1671
NdeII	GATC	9 cut(s)	814, 976, 1002, 1057, 1699, 1733, 1884, 1954, 2108
NgoMIV	GCCGGC	1 cut(s)	436
NlaIII	CATG	5 cut(s)	48, 892, 1175, 1505, 1667
NlaIV	GGNNCC	4 cut(s)	93, 869, 1701, 2215
NmeAIII	GCCGAG	1 cut(s)	2078
NmuCI	GTSAC	2 cut(s)	1738, 1925
NspI	RCATGY	2 cut(s)	892, 1505
NspV	TTCGAA	1 cut(s)	347
OliI	CACNNNNGTG	1 cut(s)	917
PaeI	GCATGC	1 cut(s)	892
PagI	TCATGA	1 cut(s)	1663
PceI	AGGCCT	1 cut(s)	239
PciI	ACATGT	1 cut(s)	1501
PcsI	WCGNNNNNNNCGW	5 cut(s)	95, 1345, 1354, 1377, 1963
PdiI	GCCGGC	1 cut(s)	438
PdmI	GAANNNNTTC	1 cut(s)	855
PfeI	GAWTC	4 cut(s)	505, 635, 1319, 1535
PfoI	TCCNGGA	1 cut(s)	1864
PleI	GAGTC	5 cut(s)	34, 272, 292, 304, 1495
PpsI	GAGTC	5 cut(s)	34, 272, 292, 304, 1495
Ppu21I	YACGTR	1 cut(s)	685
PscI	ACATGT	1 cut(s)	1501
PshAI	GACNNNNGTC	1 cut(s)	1639
PsiI	TTATAA	1 cut(s)	1064
Psp1406I	AACGTT	1 cut(s)	1284
Psp6I	CCWGG	2 cut(s)	736, 1864
PspEI	GGTNACC	1 cut(s)	1718
PspFI	CCCAGC	1 cut(s)	871
PspGI	CCWGG	2 cut(s)	736, 1864
PspN4I	GGNNCC	4 cut(s)	93, 869, 1701, 2215
PspPI	GGNCC	4 cut(s)	91, 355, 583, 1916
PstI	CTGCAG	1 cut(s)	1771
PstNI	CAGNNNCTG	2 cut(s)	1570, 1775
PsuI	RGATCY	3 cut(s)	1699, 1733, 2108
PvuII	CAGCTG	1 cut(s)	1772
RsaI	GTAC	4 cut(s)	801, 1335, 1351, 2170
RsaNI	GTAC	4 cut(s)	800, 1334, 1350, 2169
RseI	CAYNNNNRTG	2 cut(s)	917, 2254
SalI	GTCGAC	1 cut(s)	1965
SaqAI	TTAA	5 cut(s)	516, 630, 1209, 1799, 2103
Sau3AI	GATC	9 cut(s)	814, 976, 1002, 1057, 1699, 1733, 1884, 1954, 2108
Sau96I	GGNCC	4 cut(s)	91, 355, 583, 1916
SchI	GAGTC	5 cut(s)	34, 272, 293, 305, 1496
ScrFI	CCNGG	5 cut(s)	95, 738, 1648, 1671, 1866
SduI	GDGCHC	4 cut(s)	228, 703, 1242, 2244
SfaNI	GCATC	5 cut(s)	810, 1108, 1268, 1430, 2258
SfcI	CTRYAG	2 cut(s)	75, 1767
SfuI	TTCGAA	1 cut(s)	347
SinI	GGWCC	1 cut(s)	583
SmiMI	CAYNNNNRTG	2 cut(s)	917, 2254
SmlI	CTYRAG	3 cut(s)	383, 773, 1424
SmoI	CTYRAG	3 cut(s)	383, 773, 1424
SnaBI	TACGTA	1 cut(s)	685
SphI	GCATGC	1 cut(s)	892
Sse9I	AATT	7 cut(s)	217, 350, 855, 1229, 1692, 1800, 2224
SseBI	AGGCCT	1 cut(s)	239
SspI	AATATT	2 cut(s)	794, 941
StuI	AGGCCT	1 cut(s)	239
StyD4I	CCNGG	5 cut(s)	93, 736, 1646, 1669, 1864
StyI	CCWWGG	1 cut(s)	264
TaaI	ACNGT	8 cut(s)	167, 658, 1069, 1237, 1414, 1471, 1545, 1874
TaiI	ACGT	5 cut(s)	687, 723, 1117, 1287, 1360
TaqI	TCGA	4 cut(s)	8, 347, 451, 1966
TasI	AATT	7 cut(s)	217, 350, 855, 1229, 1692, 1800, 2224
TatI	WGTACW	1 cut(s)	1333
TauI	GCSGC	6 cut(s)	71, 114, 117, 120, 768, 2052
TfiI	GAWTC	4 cut(s)	505, 635, 1319, 1535
Tru1I	TTAA	5 cut(s)	516, 630, 1209, 1799, 2103
Tru9I	TTAA	5 cut(s)	516, 630, 1209, 1799, 2103
TseFI	GTSAC	2 cut(s)	1738, 1925
TseI	GCWGC	8 cut(s)	65, 768, 1601, 1604, 1769, 1772, 2115, 2118
Tsp45I	GTSAC	2 cut(s)	1738, 1925
TspDTI	ATGAA	8 cut(s)	552, 669, 822, 1140, 1320, 1517, 1794, 1824
TspGWI	ACGGA	1 cut(s)	1629
VpaK11BI	GGWCC	1 cut(s)	583
XapI	RAATTY	1 cut(s)	1229
XceI	RCATGY	2 cut(s)	892, 1505
XcmI	CCANNNNNNNNNTGG	2 cut(s)	271, 1288
XmiI	GTMKAC	1 cut(s)	1966
XmnI	GAANNNNTTC	1 cut(s)	855

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.