Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0008791

Physical Location & Seq

Genomic Coordinates Reverse (-)

27472879 .. 27477467

Gene Structure 4589 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1183400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2295 bp

ATGTCTTCTATCATCGGAGTTTCCTCTGTATATTCCCAGACTCCGTGCAGCGAGCTGTACAGAAGAGCTGCGCCGGCGGCGGCAACGACGAGTAGTGCTACTCTTTCGCTTGCGTTCCCGGAGAGGGCGCACTTCAACAGTGTTTTGAGAGGTAAGAGTGGGTTGGTCAGGCAGGGGTCCGGTGTCGTTTGTTCGGCAATTGCGACTCCGAACTCGGTGCTCAGTGAAGAGGCGTTTAAGGGACTCGGTGACTTTTCGAAGGACGGCCTCGACTCGGACTTGGAGTACGAGTCGGAAACCGAGCCGGGTTCTGCTGCCAGTGACGACGATGAGCTCGCCCTTTCCAAGCTGGGTTTGCCTCAGCGGCTTCTTGATAGCCTTGAGAGACGTGGCATATCGAACCTTTTCCCAATTCAGAGAGCTGTTCTAGTACCTGCACTTGAAGGTCGAGATATCATTGCGCGTGCCAAGACAGGGACTGGAAAGACATTAGCCTTCGGAATCCCAATACTCAAGCGTCTCACAGAAGATGATGAACAGAGAGGCTCTCATAGGCGGACTGGTTATCTTCCAAGAGTTTTGGTGCTTGCACCTACTCGGGAGTTGGCAAAGCAAGTCGAAAAGGAGATGAAAGAATCTGCACCTTATCTTAACACCGTTTGTGTCTATGGGGGAGTTTCCTACATAACTCAACAAAGTGCTCTCTCCCGTGGAGTTGATGTAGTTGTTGGAACTCCTGGTCGGATTATTGACCTGATAAACGGCAACACCCTCAAATTGGGCGAAGTACAATATTTGGTACTTGATGAAGCTGATTCAATGCTTGCTGTTGGATTTGAGGAGGATGTGGAAGTGATTCTGCAAAAGCTTCCAGCTCAGAGGCAGAGCATGCTTTTTTCTGCAACCATGCCTGGTTGGGTGAAAAAACTATCACGGAAATATTTGGACAATCCAATGACAATTGATTTGGTTGGCGACCAAGACGAGAAGCTTGCAGAAGGGATCAAACTTTATGCTTTATCAACCACTTCAGCTTCAAAGAAGACCATTCTTAGTGATCTTATAACGGTGTACGCAAAGGGTGGGAAGACCATTGTATTTACACAAACAAAACGAGATGCTGATGAAGTCTCAATGTCATTAACAAGTAGCATAGCTTCCGAGGCATTGCATGGAGATATATCACAGCATCAAAGAGAGAGAACATTAAATGGTTTTCGACAAGGGAAATTCACTGTGCTCGTTGCCACTGATGTTGCATCCCGTGGACTTGATATTCCCAATGTTGATTTGGTTATCCACTACGAGCTTCCCAATGATTCAGAGACATTTGTGCATCGCTCTGGGCGTACTGGACGCGCAGGGAAAGAAGGTACTGCCGTTCTGATGTTTACTAACAACCAGAGGAGAACAATTAAAACGCTTGAGCGTGATGTGGGGTGCAAGTTTGAGTTTGCTAGTCCACCAACTATTGAGGAGGTTTTGGAGTCATCTGCGCAGCATGTGGTTGCTACTCTTAGTGGAGTGCATCCTGAATCTGTACAGTTTTTCACACCAACAGCACAGAGATTGATTGATGAACAGGGAACAAGTGCTCTTGCTGCTGCACTAGCACAGCTCAGTGGATTTTCTCGACCTCCATCATCCAAGTCCCTTATCACCCATGAGGCGGGATGGACGACATTGCAACTTATCCGAGATCCATCTTTTGCCAGAGGGTTCCTGTCTGCTAGATCTGTCACTGGGTTTCTTTCAGATGTTTATTCTACGGCTGCTGATGAAGTTGGAAAAATACATATAATTGCAGATGAACGGATTCAAGGAGCAGTCTTTGATCTTCCAGAGGAGATTGCCAAAGAGTTGCTGACTAGGCAAATACCTCCTGGAAACACCGTTTCCAAGATATCCAAGTTGCCAGCCTTGCAAGATGATGGGCCGGTGAGTGACTACTACGGCAGGTTTTCAAGCAGAGATCGCCGAGGAGGAGGCCGTGGAGGAGGCCGGGGAGGAGGTTCTAGGGATCGTCAAGGATCATCAGGTGGTTTCAGAAGCTCCAGGGGTTGGGGTAGTGATGGTGGTGCCGACGATTCATTTAGGAGTAGTGGCCGAAGTTATGGATCTAGCAACAGCAGACCCCGGAGTCCAAGAAGCATCGATGACGATTGGCTTATTGGAGGCCAACGATCAAGCAGGTCACCATCCCGAGACAGTTCCAGAAGCTTTGGAGGTTCCTGCTTCAATTGTGGGCAGTCTGGCCACAGGGCATCGGATTGCCCTACCAAACAAGGATTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000373 GO:0000375 GO:0000377 GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003729 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006970 GO:0006996 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009628 GO:0009651 GO:0009657 GO:0009658 GO:0009941 GO:0009987 GO:0010467 GO:0010501 GO:0016020 GO:0016043 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022613 GO:0031967 GO:0031974 GO:0031975 GO:0031981 GO:0034641 GO:0042254 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0050896 GO:0070013 GO:0070035 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko01110 map01110

Pfam Domains

Protein Families

DEAD GUCT Helicase_C zf-CCHC

Protein Analysis

765

Amino Acids

82.31

Weight (kDa)

7.18

Isoelectric Point (pI)

46.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	137 - 309	6.5e-45	DEAD/DEAH box helicase
ResIII	PF04851	150 - 303	1.5e-06	Type III restriction enzyme, res subunit
Helicase_C	PF00271	347 - 455	7.6e-32	Helicase conserved C-terminal domain
DD_DDX21-DDX50	PF26142	491 - 539	1.2e-15	DDX21/DDX50 dimerisation domain
GUCT	PF08152	542 - 642	1.8e-14	GUCT (NUC152) domain
zf-CCHC	PF00098	744 - 759	6e-07	Zinc knuckle

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008791)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G26742 AT5G26742 AT5G26742
fragaria_vesca	FvH4_6g08810 FvH4_6g08810 FvH4_6g08810 FvH4_6g08810
malus_domestica	MD04G1183400.v1.1 MD12G1196800.v1.1
prunus_persica	Prupe.6G301900_v2.0.a1
pyrus_communis	pycom04g16190 pycom12g18300
rosa_chinensis	RchiOBHm_Chr3g0459081
rosa_laevigata	RLG00000025121
rosa_multiflora	Rmu_sc0000466.1_g000002 Rmu_sc0003515.1_g000001
rosa_roxburghii	Rroxscaffold_6G00420530
rosa_rugosa	Rorug03G0033400
rosa_samantha	Rh3AG091300 Rh3BG094400 Rh3CG094800 Rh3DG094800
rosa_wichuraiana	Rw3G007840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1064
AasI	GACNNNNNNGTC	1 cut(s)	2139
Acc16I	TGCGCA	1 cut(s)	1497
Acc36I	ACCTGC	3 cut(s)	442, 1947, 2181
AccB1I	GGYRCC	1 cut(s)	2078
AccB7I	CCANNNNNTGG	1 cut(s)	2061
AccII	CGCG	2 cut(s)	463, 1359
AciI	CCGC	5 cut(s)	77, 80, 364, 556, 1670
AclWI	GGATC	5 cut(s)	1010, 1694, 2028, 2038, 2125
AcoI	YGGCCR	2 cut(s)	2104, 2254
AcsI	RAATTY	1 cut(s)	1229
AcuI	CTGAAG	1 cut(s)	1014
AfaI	GTAC	9 cut(s)	59, 287, 432, 789, 801, 1073, 1351, 1375, 1542
AfiI	CCNNNNNNNGG	6 cut(s)	124, 274, 474, 778, 1669, 2061
AgsI	TTSAA	7 cut(s)	136, 443, 819, 1038, 1820, 1965, 2239
AjiI	CACGTC	1 cut(s)	389
AjnI	CCWGG	4 cut(s)	736, 910, 1882, 2054
AjuI	GAANNNNNNNTTGG	1 cut(s)	2273
Alw21I	GWGCWC	5 cut(s)	222, 336, 703, 1242, 1597
Alw26I	GTCTC	5 cut(s)	379, 524, 1135, 1319, 2199
AlwI	GGATC	5 cut(s)	1010, 1694, 2028, 2038, 2125
AlwNI	CAGNNNCTG	1 cut(s)	479
Ama87I	CYCGRG	2 cut(s)	597, 2202
AoxI	GGCC	7 cut(s)	265, 1934, 1987, 1999, 2104, 2176, 2254
ApeKI	GCWGC	7 cut(s)	48, 68, 314, 1498, 1601, 1604, 1772
ApoI	RAATTY	1 cut(s)	1229
ArsI	GACNNNNNNTTYG	2 cut(s)	949, 981
Asp700I	GAANNNNTTC	3 cut(s)	404, 855, 1815
AspLEI	GCGC	5 cut(s)	73, 130, 463, 1361, 1498
AspS9I	GGNCC	2 cut(s)	177, 1934
AsuC2I	CCSGG	4 cut(s)	119, 306, 2003, 2137
AsuHPI	GGTGA	5 cut(s)	260, 931, 1651, 1951, 2187
AsuII	TTCGAA	1 cut(s)	257
AvaI	CYCGRG	2 cut(s)	597, 2202
AvaII	GGWCC	1 cut(s)	177
BaeI	ACNNNNGTAYC	2 cut(s)	1365, 1398
BalI	TGGCCA	1 cut(s)	2256
BanI	GGYRCC	1 cut(s)	2078
BanII	GRGCYC	1 cut(s)	336
BarI	GAAGNNNNNNTAC	2 cut(s)	1141, 1173
BbsI	GAAGAC	2 cut(s)	1049, 1094
Bbv12I	GWGCWC	5 cut(s)	222, 336, 703, 1242, 1597
BbvCI	CCTCAGC	1 cut(s)	360
BbvI	GCAGC	7 cut(s)	55, 60, 301, 1510, 1588, 1591, 1759
BccI	CCATC	6 cut(s)	1648, 1668, 1711, 1925, 2066, 2206
BceAI	ACGGC	6 cut(s)	280, 778, 1364, 1785, 1969, 1974
BcgI	CGANNNNNNTGC	4 cut(s)	199, 233, 974, 1008
BciT130I	CCWGG	4 cut(s)	738, 912, 1884, 2056
BcnI	CCSGG	4 cut(s)	119, 306, 2003, 2137
BcoDI	GTCTC	5 cut(s)	379, 524, 1135, 1319, 2199
BfaI	CTAG	8 cut(s)	428, 1458, 1610, 1731, 1869, 2016, 2121, 2293
BfuAI	ACCTGC	3 cut(s)	442, 1947, 2181
BglI	GCCNNNNNGGC	1 cut(s)	364
BglII	AGATCT	1 cut(s)	1733
Bme1390I	CCNGG	8 cut(s)	119, 306, 738, 912, 1884, 2003, 2056, 2137
Bme18I	GGWCC	1 cut(s)	177
BmeT110I	CYCGRG	2 cut(s)	597, 2202
BmgBI	CACGTC	1 cut(s)	389
BmgT120I	GGNCC	2 cut(s)	177, 1934
BmiI	GGNNCC	4 cut(s)	178, 1721, 2080, 2230
BmrFI	CCNGG	8 cut(s)	119, 306, 738, 912, 1884, 2003, 2056, 2137
BmrI	ACTGGG	1 cut(s)	1752
BmsI	GCATC	7 cut(s)	1108, 1198, 1268, 1345, 1537, 2160, 2273
BmuI	ACTGGG	1 cut(s)	1752
BpiI	GAAGAC	2 cut(s)	1049, 1094
BplI	GAGNNNNNCTC	2 cut(s)	532, 564
BpmI	CTGGAG	1 cut(s)	2038
Bpu10I	CCTNAGC	1 cut(s)	360
Bpu14I	TTCGAA	1 cut(s)	257
BpuEI	CTTGAG	3 cut(s)	401, 497, 1445
BpuMI	CCSGG	4 cut(s)	119, 306, 2003, 2137
Bsa29I	ATCGAT	1 cut(s)	2154
BsaJI	CCNNGG	8 cut(s)	709, 1161, 1264, 1978, 1990, 2002, 2055, 2135
BsaWI	WCCGGW	1 cut(s)	179
BsaXI	ACNNNNNCTCC	4 cut(s)	275, 305, 705, 735
Bsc4I	CCNNNNNNNGG	6 cut(s)	124, 274, 474, 778, 1669, 2061
Bse118I	RCCGGY	2 cut(s)	73, 1936
Bse1I	ACTGG	5 cut(s)	318, 484, 565, 1357, 1747
Bse3DI	GCAATG	3 cut(s)	456, 1166, 1682
BseBI	CCWGG	4 cut(s)	738, 912, 1884, 2056
BseCI	ATCGAT	1 cut(s)	2154
BseDI	CCNNGG	8 cut(s)	709, 1161, 1264, 1978, 1990, 2002, 2055, 2135
BseGI	GGATG	6 cut(s)	850, 1259, 1528, 1643, 1679, 2198
BseLI	CCNNNNNNNGG	6 cut(s)	124, 274, 474, 778, 1669, 2061
BseMI	GCAATG	3 cut(s)	456, 1166, 1682
BseMII	CTCAG	4 cut(s)	235, 374, 890, 1633
BseNI	ACTGG	5 cut(s)	318, 484, 565, 1357, 1747
BseRI	GAGGAG	8 cut(s)	854, 1420, 1490, 1859, 1995, 1998, 2010, 2022
BseXI	GCAGC	7 cut(s)	55, 60, 301, 1510, 1588, 1591, 1759
BseYI	CCCAGC	1 cut(s)	349
BsgI	GTGCAG	4 cut(s)	67, 420, 624, 1590
Bsh1236I	CGCG	2 cut(s)	463, 1359
BshFI	GGCC	7 cut(s)	267, 1936, 1989, 2001, 2106, 2178, 2256
BshNI	GGYRCC	1 cut(s)	2078
BshVI	ATCGAT	1 cut(s)	2154
BsiHKAI	GWGCWC	5 cut(s)	222, 336, 703, 1242, 1597
BsiHKCI	CYCGRG	2 cut(s)	597, 2202
BsiSI	CCGG	7 cut(s)	74, 119, 180, 305, 1937, 2002, 2137
BslFI	GGGAC	3 cut(s)	255, 490, 1636
BslI	CCNNNNNNNGG	6 cut(s)	124, 274, 474, 778, 1669, 2061
BsmAI	GTCTC	5 cut(s)	379, 524, 1135, 1319, 2199
BsmBI	CGTCTC	2 cut(s)	379, 524
BsmFI	GGGAC	3 cut(s)	255, 490, 1636
BsnI	GGCC	7 cut(s)	267, 1936, 1989, 2001, 2106, 2178, 2256
BsoBI	CYCGRG	2 cut(s)	597, 2202
Bsp119I	TTCGAA	1 cut(s)	257
Bsp1286I	GDGCHC	5 cut(s)	222, 336, 703, 1242, 1597
Bsp1407I	TGTACA	2 cut(s)	57, 1540
BspACI	CCGC	5 cut(s)	77, 80, 364, 556, 1670
BspANI	GGCC	7 cut(s)	267, 1936, 1989, 2001, 2106, 2178, 2256
BspCNI	CTCAG	4 cut(s)	234, 373, 889, 1632
BspDI	ATCGAT	1 cut(s)	2154
BspFNI	CGCG	2 cut(s)	463, 1359
BspLI	GGNNCC	4 cut(s)	178, 1721, 2080, 2230
BspMI	ACCTGC	3 cut(s)	442, 1947, 2181
BspPI	GGATC	5 cut(s)	1010, 1694, 2028, 2038, 2125
BspQI	GCTCTTC	1 cut(s)	58
BspT104I	TTCGAA	1 cut(s)	257
BspT107I	GGYRCC	1 cut(s)	2078
BsrDI	GCAATG	3 cut(s)	456, 1166, 1682
BsrFI	RCCGGY	2 cut(s)	73, 1936
BsrGI	TGTACA	2 cut(s)	57, 1540
BsrI	ACTGG	5 cut(s)	318, 484, 565, 1357, 1747
BssAI	RCCGGY	2 cut(s)	73, 1936
BssECI	CCNNGG	8 cut(s)	709, 1161, 1264, 1978, 1990, 2002, 2055, 2135
Bst2UI	CCWGG	4 cut(s)	738, 912, 1884, 2056
Bst4CI	ACNGT	7 cut(s)	140, 658, 1069, 1237, 1545, 1894, 2210
Bst6I	CTCTTC	2 cut(s)	58, 222
BstAPI	GCANNNNNTGC	1 cut(s)	889
BstAUI	TGTACA	2 cut(s)	57, 1540
BstBI	TTCGAA	1 cut(s)	257
BstDEI	CTNAG	6 cut(s)	221, 360, 876, 1052, 1517, 1619
BstDSI	CCRYGG	3 cut(s)	709, 1264, 1990
BstEII	GGTNACC	1 cut(s)	2193
BstF5I	GGATG	6 cut(s)	850, 1259, 1528, 1643, 1679, 2198
BstFNI	CGCG	2 cut(s)	463, 1359
BstHHI	GCGC	5 cut(s)	73, 130, 463, 1361, 1498
BstMAI	GTCTC	5 cut(s)	379, 524, 1135, 1319, 2199
BstNI	CCWGG	4 cut(s)	738, 912, 1884, 2056
BstNSI	RCATGY	2 cut(s)	892, 1505
BstPI	GGTNACC	1 cut(s)	2193
BstSCI	CCNGG	8 cut(s)	117, 304, 736, 910, 1882, 2001, 2054, 2135
BstUI	CGCG	2 cut(s)	463, 1359
BstV1I	GCAGC	7 cut(s)	55, 60, 301, 1510, 1588, 1591, 1759
BstV2I	GAAGAC	2 cut(s)	1049, 1094
BstX2I	RGATCY	3 cut(s)	1699, 1733, 2117
BstYI	RGATCY	3 cut(s)	1699, 1733, 2117
Bsu15I	ATCGAT	1 cut(s)	2154
BsuRI	GGCC	7 cut(s)	267, 1936, 1989, 2001, 2106, 2178, 2256
BsuTUI	ATCGAT	1 cut(s)	2154
BtgI	CCRYGG	3 cut(s)	709, 1264, 1990
BtgZI	GCGATG	1 cut(s)	1322
BtrI	CACGTC	1 cut(s)	389
BtsCI	GGATG	6 cut(s)	850, 1259, 1528, 1643, 1679, 2198
BtsIMutI	CAGTG	7 cut(s)	145, 229, 325, 1233, 1248, 1627, 1740
BveI	ACCTGC	3 cut(s)	442, 1947, 2181
CaiI	CAGNNNCTG	1 cut(s)	479
CfoI	GCGC	5 cut(s)	73, 130, 463, 1361, 1498
Cfr10I	RCCGGY	2 cut(s)	73, 1936
Cfr13I	GGNCC	2 cut(s)	177, 1934
ClaI	ATCGAT	1 cut(s)	2154
CseI	GACGC	2 cut(s)	506, 1365
Csp6I	GTAC	9 cut(s)	58, 286, 431, 788, 800, 1072, 1350, 1374, 1541
CspCI	CAANNNNNGTGG	2 cut(s)	1237, 1272
CviAII	CATG	5 cut(s)	889, 907, 1172, 1502, 1664
CviQI	GTAC	9 cut(s)	58, 286, 431, 788, 800, 1072, 1350, 1374, 1541
DdeI	CTNAG	6 cut(s)	221, 360, 876, 1052, 1517, 1619
DrdI	GACNNNNNNGTC	1 cut(s)	2139
DseDI	GACNNNNNNGTC	1 cut(s)	2139
EaeI	YGGCCR	2 cut(s)	2104, 2254
Eam1104I	CTCTTC	2 cut(s)	58, 222
EarI	CTCTTC	2 cut(s)	58, 222
EciI	GGCGGA	1 cut(s)	571
Ecl136II	GAGCTC	1 cut(s)	334
Eco24I	GRGCYC	1 cut(s)	336
Eco32I	GATATC	2 cut(s)	454, 1905
Eco47I	GGWCC	1 cut(s)	177
Eco53kI	GAGCTC	1 cut(s)	334
Eco57I	CTGAAG	1 cut(s)	1014
Eco88I	CYCGRG	2 cut(s)	597, 2202
Eco91I	GGTNACC	1 cut(s)	2193
EcoICRI	GAGCTC	1 cut(s)	334
EcoO65I	GGTNACC	1 cut(s)	2193
EcoRII	CCWGG	4 cut(s)	736, 910, 1882, 2054
EcoRV	GATATC	2 cut(s)	454, 1905
EcoT38I	GRGCYC	1 cut(s)	336
Esp3I	CGTCTC	2 cut(s)	379, 524
FaeI	CATG	5 cut(s)	892, 910, 1175, 1505, 1667
FaqI	GGGAC	3 cut(s)	255, 490, 1636
FatI	CATG	5 cut(s)	888, 906, 1171, 1501, 1663
FauI	CCCGC	1 cut(s)	1663
FokI	GGATG	6 cut(s)	857, 1246, 1515, 1630, 1686, 2185
FriOI	GRGCYC	1 cut(s)	336
FspBI	CTAG	8 cut(s)	428, 1458, 1610, 1731, 1869, 2016, 2121, 2293
FspI	TGCGCA	1 cut(s)	1497
GlaI	GCGC	5 cut(s)	72, 129, 462, 1360, 1497
GsaI	CCCAGC	1 cut(s)	353
GsuI	CTGGAG	1 cut(s)	2038
HaeIII	GGCC	7 cut(s)	267, 1936, 1989, 2001, 2106, 2178, 2256
HapII	CCGG	7 cut(s)	74, 119, 180, 305, 1937, 2002, 2137
HgaI	GACGC	2 cut(s)	506, 1365
HhaI	GCGC	5 cut(s)	73, 130, 463, 1361, 1498
Hin1II	CATG	5 cut(s)	892, 910, 1175, 1505, 1667
Hin6I	GCGC	5 cut(s)	71, 128, 461, 1359, 1496
HinP1I	GCGC	5 cut(s)	71, 128, 461, 1359, 1496
HindIII	AAGCTT	3 cut(s)	866, 989, 2218
HpaII	CCGG	7 cut(s)	74, 119, 180, 305, 1937, 2002, 2137
HphI	GGTGA	5 cut(s)	260, 931, 1651, 1951, 2187
Hpy166II	GTNNAC	4 cut(s)	1072, 1268, 1392, 1463
Hpy188III	TCNNGA	8 cut(s)	371, 449, 599, 1532, 1632, 1841, 2202, 2214
Hpy8I	GTNNAC	4 cut(s)	1072, 1268, 1392, 1463
Hpy99I	CGWCG	3 cut(s)	91, 329, 2087
HpyAV	CCTTC	5 cut(s)	253, 437, 505, 992, 1364
HpyCH4III	ACNGT	7 cut(s)	140, 658, 1069, 1237, 1545, 1894, 2210
HpyCH4IV	ACGT	1 cut(s)	388
HpyF3I	CTNAG	6 cut(s)	221, 360, 876, 1052, 1517, 1619
HpySE526I	ACGT	1 cut(s)	388
Hsp92II	CATG	5 cut(s)	892, 910, 1175, 1505, 1667
HspAI	GCGC	5 cut(s)	71, 128, 461, 1359, 1496
KroI	GCCGGC	1 cut(s)	73
KroNI	GCCGGC	1 cut(s)	75
LguI	GCTCTTC	1 cut(s)	58
LmnI	GCTCC	2 cut(s)	1823, 2057
Lsp1109I	GCAGC	7 cut(s)	55, 60, 301, 1510, 1588, 1591, 1759
LweI	GCATC	7 cut(s)	1108, 1198, 1268, 1345, 1537, 2160, 2273
MaeI	CTAG	8 cut(s)	428, 1458, 1610, 1731, 1869, 2016, 2121, 2293
MaeII	ACGT	1 cut(s)	388
MaeIII	GTNAC	5 cut(s)	248, 320, 1738, 1943, 2193
MboII	GAAGA	7 cut(s)	75, 239, 539, 560, 1054, 1099, 1829
MfeI	CAATTG	3 cut(s)	198, 960, 2239
MflI	RGATCY	3 cut(s)	1699, 1733, 2117
MhlI	GDGCHC	5 cut(s)	222, 336, 703, 1242, 1597
MlsI	TGGCCA	1 cut(s)	2256
MluCI	AATT	8 cut(s)	198, 411, 776, 960, 1229, 1413, 1800, 2239
MluNI	TGGCCA	1 cut(s)	2256
MlyI	GAGTC	7 cut(s)	34, 199, 237, 266, 299, 1496, 2149
MmeI	TCCRAC	5 cut(s)	273, 709, 722, 811, 1765
Mox20I	TGGCCA	1 cut(s)	2256
MreI	CGCCGGCG	1 cut(s)	73
MroNI	GCCGGC	1 cut(s)	73
MroXI	GAANNNNTTC	3 cut(s)	404, 855, 1815
MscI	TGGCCA	1 cut(s)	2256
MseI	TTAA	5 cut(s)	237, 651, 1142, 1208, 1416
Msp20I	TGGCCA	1 cut(s)	2256
MspA1I	CMGCKG	1 cut(s)	364
MspI	CCGG	7 cut(s)	74, 119, 180, 305, 1937, 2002, 2137
MspR9I	CCNGG	8 cut(s)	119, 306, 738, 912, 1884, 2003, 2056, 2137
MunI	CAATTG	3 cut(s)	198, 960, 2239
MvaI	CCWGG	4 cut(s)	738, 912, 1884, 2056
MvnI	CGCG	2 cut(s)	463, 1359
NaeI	GCCGGC	1 cut(s)	75
NciI	CCSGG	4 cut(s)	119, 306, 2003, 2137
NgoMIV	GCCGGC	1 cut(s)	73
NlaIII	CATG	5 cut(s)	892, 910, 1175, 1505, 1667
NlaIV	GGNNCC	4 cut(s)	178, 1721, 2080, 2230
NmeAIII	GCCGAG	1 cut(s)	2003
NmuCI	GTSAC	5 cut(s)	248, 320, 1738, 1943, 2193
NsbI	TGCGCA	1 cut(s)	1497
NspI	RCATGY	2 cut(s)	892, 1505
NspV	TTCGAA	1 cut(s)	257
PaeI	GCATGC	1 cut(s)	892
PciSI	GCTCTTC	1 cut(s)	58
PcsI	WCGNNNNNNNCGW	2 cut(s)	200, 1345
PdiI	GCCGGC	1 cut(s)	75
PdmI	GAANNNNTTC	3 cut(s)	404, 855, 1815
PfeI	GAWTC	9 cut(s)	501, 635, 815, 856, 1319, 1535, 1816, 2087, 2289
PflMI	CCANNNNNTGG	1 cut(s)	2061
PfoI	TCCNGGA	2 cut(s)	117, 1882
PleI	GAGTC	7 cut(s)	34, 199, 237, 266, 298, 1495, 2148
PpsI	GAGTC	7 cut(s)	34, 199, 237, 266, 298, 1495, 2148
PsiI	TTATAA	1 cut(s)	1064
Psp124BI	GAGCTC	1 cut(s)	336
Psp6I	CCWGG	4 cut(s)	736, 910, 1882, 2054
PspEI	GGTNACC	1 cut(s)	2193
PspFI	CCCAGC	1 cut(s)	349
PspGI	CCWGG	4 cut(s)	736, 910, 1882, 2054
PspN4I	GGNNCC	4 cut(s)	178, 1721, 2080, 2230
PspPI	GGNCC	2 cut(s)	177, 1934
PstNI	CAGNNNCTG	1 cut(s)	479
PsuI	RGATCY	3 cut(s)	1699, 1733, 2117
RsaI	GTAC	9 cut(s)	59, 287, 432, 789, 801, 1073, 1351, 1375, 1542
RsaNI	GTAC	9 cut(s)	58, 286, 431, 788, 800, 1072, 1350, 1374, 1541
SacI	GAGCTC	1 cut(s)	336
SapI	GCTCTTC	1 cut(s)	58
SaqAI	TTAA	5 cut(s)	237, 651, 1142, 1208, 1416
Sau96I	GGNCC	2 cut(s)	177, 1934
SchI	GAGTC	7 cut(s)	34, 199, 237, 266, 299, 1496, 2149
ScrFI	CCNGG	8 cut(s)	119, 306, 738, 912, 1884, 2003, 2056, 2137
SduI	GDGCHC	5 cut(s)	222, 336, 703, 1242, 1597
SfaNI	GCATC	7 cut(s)	1108, 1198, 1268, 1345, 1537, 2160, 2273
SfuI	TTCGAA	1 cut(s)	257
SgrAI	CRCCGGYG	1 cut(s)	73
SinI	GGWCC	1 cut(s)	177
SmlI	CTYRAG	3 cut(s)	380, 512, 1424
SmoI	CTYRAG	3 cut(s)	380, 512, 1424
SphI	GCATGC	1 cut(s)	892
Sse9I	AATT	8 cut(s)	198, 411, 776, 960, 1229, 1413, 1800, 2239
SsiI	CCGC	5 cut(s)	77, 80, 364, 556, 1670
SspI	AATATT	2 cut(s)	794, 941
SspMI	CTAG	8 cut(s)	428, 1458, 1610, 1731, 1869, 2016, 2121, 2293
SstI	GAGCTC	1 cut(s)	336
StyD4I	CCNGG	8 cut(s)	117, 304, 736, 910, 1882, 2001, 2054, 2135
TaaI	ACNGT	7 cut(s)	140, 658, 1069, 1237, 1545, 1894, 2210
TaiI	ACGT	1 cut(s)	391
TaqI	TCGA	8 cut(s)	257, 270, 398, 448, 618, 1219, 1633, 2154
TasI	AATT	8 cut(s)	198, 411, 776, 960, 1229, 1413, 1800, 2239
TatI	WGTACW	3 cut(s)	57, 787, 1540
TauI	GCSGC	3 cut(s)	80, 83, 367
TfiI	GAWTC	9 cut(s)	501, 635, 815, 856, 1319, 1535, 1816, 2087, 2289
Tru1I	TTAA	5 cut(s)	237, 651, 1142, 1208, 1416
Tru9I	TTAA	5 cut(s)	237, 651, 1142, 1208, 1416
TscAI	CASTG	7 cut(s)	145, 229, 325, 1240, 1255, 1627, 1747
TseFI	GTSAC	5 cut(s)	248, 320, 1738, 1943, 2193
TseI	GCWGC	7 cut(s)	48, 68, 314, 1498, 1601, 1604, 1772
Tsp45I	GTSAC	5 cut(s)	248, 320, 1738, 1943, 2193
TspDTI	ATGAA	8 cut(s)	549, 644, 822, 1140, 1593, 1794, 1824, 2079
TspGWI	ACGGA	3 cut(s)	33, 949, 1828
TspRI	CASTG	7 cut(s)	145, 229, 325, 1240, 1255, 1627, 1747
Van91I	CCANNNNNTGG	1 cut(s)	2061
VpaK11BI	GGWCC	1 cut(s)	177
XapI	RAATTY	1 cut(s)	1229
XceI	RCATGY	2 cut(s)	892, 1505
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1288
XmnI	GAANNNNTTC	3 cut(s)	404, 855, 1815
XspI	CTAG	8 cut(s)	428, 1458, 1610, 1731, 1869, 2016, 2121, 2293

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.